CN1062450C - Formula and preparation process of lactobacillaceau embolus - Google Patents
Formula and preparation process of lactobacillaceau embolus Download PDFInfo
- Publication number
- CN1062450C CN1062450C CN96117361A CN96117361A CN1062450C CN 1062450 C CN1062450 C CN 1062450C CN 96117361 A CN96117361 A CN 96117361A CN 96117361 A CN96117361 A CN 96117361A CN 1062450 C CN1062450 C CN 1062450C
- Authority
- CN
- China
- Prior art keywords
- lactobacillus
- suppository
- gelatin
- preparation
- glycerol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000186660 Lactobacillus Species 0.000 claims abstract description 35
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 35
- 239000006216 vaginal suppository Substances 0.000 claims abstract description 4
- 229940120293 vaginal suppository Drugs 0.000 claims abstract description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 27
- 238000002360 preparation method Methods 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 11
- 239000000829 suppository Substances 0.000 claims description 11
- 108010010803 Gelatin Proteins 0.000 claims description 9
- 229920000159 gelatin Polymers 0.000 claims description 9
- 239000008273 gelatin Substances 0.000 claims description 9
- 235000019322 gelatine Nutrition 0.000 claims description 9
- 235000011852 gelatine desserts Nutrition 0.000 claims description 9
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 7
- 239000000047 product Substances 0.000 claims description 7
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 6
- 239000012531 culture fluid Substances 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- 239000012467 final product Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 230000015271 coagulation Effects 0.000 claims description 3
- 238000005345 coagulation Methods 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 3
- 229910052697 platinum Inorganic materials 0.000 claims description 3
- 241000304886 Bacilli Species 0.000 claims description 2
- 235000020191 long-life milk Nutrition 0.000 claims description 2
- 238000005303 weighing Methods 0.000 claims description 2
- 239000004615 ingredient Substances 0.000 claims 1
- 210000001215 vagina Anatomy 0.000 abstract description 8
- 206010046914 Vaginal infection Diseases 0.000 abstract description 6
- 201000008100 Vaginitis Diseases 0.000 abstract description 6
- 230000003628 erosive effect Effects 0.000 abstract description 3
- 208000015181 infectious disease Diseases 0.000 abstract description 2
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 201000010099 disease Diseases 0.000 abstract 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 1
- 230000002349 favourable effect Effects 0.000 abstract 1
- 239000003814 drug Substances 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 208000004926 Bacterial Vaginosis Diseases 0.000 description 5
- 208000037009 Vaginitis bacterial Diseases 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 208000005189 Embolism Diseases 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 210000005000 reproductive tract Anatomy 0.000 description 3
- 241000405070 Percophidae Species 0.000 description 2
- 208000019802 Sexually transmitted disease Diseases 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 238000000151 deposition Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000003826 tablet Substances 0.000 description 2
- CPKVUHPKYQGHMW-UHFFFAOYSA-N 1-ethenylpyrrolidin-2-one;molecular iodine Chemical compound II.C=CN1CCCC1=O CPKVUHPKYQGHMW-UHFFFAOYSA-N 0.000 description 1
- 206010003693 Atrophic vulvovaginitis Diseases 0.000 description 1
- GHXZTYHSJHQHIJ-UHFFFAOYSA-N Chlorhexidine Chemical compound C=1C=C(Cl)C=CC=1NC(N)=NC(N)=NCCCCCCN=C(N)N=C(N)NC1=CC=C(Cl)C=C1 GHXZTYHSJHQHIJ-UHFFFAOYSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241001313288 Labia Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229920000153 Povidone-iodine Polymers 0.000 description 1
- 208000008350 Pruritus Vulvae Diseases 0.000 description 1
- 206010056530 Vulvovaginal pruritus Diseases 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 229960002227 clindamycin Drugs 0.000 description 1
- KDLRVYVGXIQJDK-AWPVFWJPSA-N clindamycin Chemical compound CN1C[C@H](CCC)C[C@H]1C(=O)N[C@H]([C@H](C)Cl)[C@@H]1[C@H](O)[C@H](O)[C@@H](O)[C@@H](SC)O1 KDLRVYVGXIQJDK-AWPVFWJPSA-N 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 210000003811 finger Anatomy 0.000 description 1
- 210000005224 forefinger Anatomy 0.000 description 1
- 230000002070 germicidal effect Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000010253 intravenous injection Methods 0.000 description 1
- 239000013010 irrigating solution Substances 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 229960000282 metronidazole Drugs 0.000 description 1
- VAOCPAMSLUNLGC-UHFFFAOYSA-N metronidazole Chemical compound CC1=NC=C([N+]([O-])=O)N1CCO VAOCPAMSLUNLGC-UHFFFAOYSA-N 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 201000010808 postmenopausal atrophic vaginitis Diseases 0.000 description 1
- 239000012286 potassium permanganate Substances 0.000 description 1
- 229960001621 povidone-iodine Drugs 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 210000003813 thumb Anatomy 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
Landscapes
- Medicinal Preparation (AREA)
Abstract
The present invention relates to a vaginal suppository prepared from live lactobacillus, which has favorable effect on treating gynecological diseases, such as bacterial vagina infection, various kinds of vaginitis, cervical erosion, etc.
Description
The present invention relates to contain the medicinal preparation of antibacterial, particularly a kind of suppository that contains lactobacillus and preparation method thereof.
Under normal circumstances, lactobacillus is keeping the balance of microecology with the anaerobe of other species, in case lactobacillus is lost ground, undue growth just appears in the latter, causes illness, causes the imbalance of vaginal flora, a kind of non-inflammation illness-bacterial vaginosis and then initiation vaginitis occur together.
Oral or intravenous injection antibiotic can make lactobacillus be impacted, and makes lactobacillus lose growth vigor, gives the bacterium of other species even the condition that pathogen has been created growth and breeding, and then reproductive tract, severe patient can cause upward reproductive tract illness under causing.
In recent years, popular along with sexually transmitted disease (STD) in the world wide, the people topical germicide that has to use in a large number, as potassium permanganate, hibitane, povidone iodine and some Chinese herbal and crude drugs preparations, its consequence is also to have killed and wounded the lactobacillus useful to body in the kill harmful bacterium, cause that lactobacillus reduces in a large number in the vagina, make other harmful bacterium be easy to breeding, cause the vagina repeated infection at vagina.
For a long time, department of obstetrics and gynecology have such creed: intravaginal disappearance lactobacillus is a synonym with suffering from bacterial vaginosis.Bacterial vaginosis is the modal gynaecopathia of women, and sickness rate is up to 33-64% among the out-patient, and treatment at present is a choice drug with metronidazole and clindamycin, and is responsive, fragile more as life-time service intravaginal environment though curative effect is good, is easy to recurrence.
Currently can see the active bacteria formulation that contains lactobacillus on market, product mainly is a yoghourt, and other some contain the product of lactobacillus such as powder, capsule, tablet, oral liquid etc., mainly are that oral formulations is that the human or animal uses.
The objective of the invention is for provide a kind of be used for bacterial vaginosis medicine sterilization after, that recovers vagina normal physiological environment contains lactobacillus suppository and preparation method thereof, therefore, the present invention has any different in prior art.
Below be the percentage by weight of each component of the present invention: gelatin 10-40% glycerol 40-75% distilled water 10-30% lactobacillus solution 5-30%
Below be processing technology of the present invention:
The preparation method of one lactobacillus liquid: the lyophilizing lactobacillus strain of going bail for and depositing with sterilization platinum earrings, change in the lactobacillus culture medium (MRS) of sterilization, cultivated 24 hours for 37 ℃, through inoculation for several times, domestication, after smear for microscopic examination is qualified, it is inoculated in the lactobacillus culture fluid of sterilization, and 37 ℃ of cultivations got final product in 24 hours.
Two suppositorys preparation: take by weighing the gelatin 10-40% in the percentage by weight, soak, make it the deliquescing of expanding with distilled water 10-30%, add glycerol 40-75% again, make gelatin, glycerol dissolving in the water-bath evenly, place in the 100-126 ℃ of pressure cooker, pressure sterilizing 10-30 minute, add lactobacillus liquid 5-30% when temperature is reduced to 50-55 ℃, make mixing, inject the vaginal suppository mould, in the refrigerator internal cooling, coagulation forming takes out packing, gets product.
The present invention is compared with the prior art: 1. lactobacillaceau embolus is specifically designed to vagina, and the domestic and international market is not seen this kind production as yet at present.2. the present invention is not the bactericidal action by medicine, but suppresses the growth of other harmful bacterium by the growth vigor of lactobacillus, thereby without any side effects.3. often kill simultaneously with germ killing drugs treatment vaginitis, cause intravaginal environment fragility, the easier growth of noxious bacteria, vaginitis recurrence together with probiotics.This product makes vagina recover physiological environment, plays a protective role, thereby cures vaginitis.
This suppository also can be used the treatment senile vaginitis with other medicines.Because of its normal probiotics that needs for additional Human Physiology, even life-time service also has no side effect.
Using method:
1. will hand-wash only, take out lactobacillaceau embolus, unpack, take out one piece.
2. get back floating position before sleeping every night, the fork both legs pinch bolt with thumb and forefinger, and separately the both sides labia is put into vaginal orifice with bolt.
3. up push away gently with one or two finger, push the vagina deep and get final product.
Consumption: one/time, once a day or follow the doctor's advice.
Below in conjunction with drawings and Examples preparation method of the present invention is illustrated.
Accompanying drawing is a process flow diagram of the present invention.
Prepare lactobacillus liquid earlier, the lyophilizing lactobacillus strain of going bail for and depositing with sterilization platinum earrings, change in the sterilized milk acidfast bacilli culture medium (MRS), cultivated 24 hours for 37 ℃, through inoculation for several times, domestication, after smear for microscopic examination is qualified, it is inoculated in the lactobacillus culture fluid of sterilization, 37 ℃ of cultivations got final product in 24 hours;
Gelatin in the percentage by weight 27 gram adding distil waters 18 grams are soaked, add 46 gram glycerol again, make the dissolving of gelatin and glycerol in the water-bath evenly, place in 115 ℃ of pressure cookers, pressure sterilizing 20 minutes becomes suppository matrix, adds lactobacillus liquid 9 grams when temperature is reduced to 50 ℃, make mixing, inject Duckbill type vaginal suppository mould, in refrigerator internal cooling, coagulation forming, take out packing, get product.
The quality standard of lactobacillaceau embolus:
Outward appearance: light brown Duckbill type; Flexible.
1.6,1.8, a 2.0g/ lactobacillus specification:: 〉=10
7Individual/g
Storage: sombre dry place gets final product, and can put for a long time in 4 ℃ of cold closetes.
In addition, also the lactobacillus of living can be prepared into irrigating solution, capsule, tablet, but all infra reproductive tract uses such as unguentum.
Select the patient of 10 routine vaginitiss and cervical erosion at random in Birth Control Institute, Tianjing City out-patient department.Everyone provides 10 pieces of lactobacillaceau embolus, and as a course of treatment, one piece of every day, everyone places continuously every night voluntarily.Clinical observation result shows: cure 1 example, produce effects 9 examples, total effective rate 100% among the 10 routine patients.The bacterial vaginosis microscopy is negative after the medication.Cervical erosion person's acceleration of healing, normal healing 7-10 days in advance.Generally improved the intravaginal environment, secretions reduces, and pruritus vulvae disappears.This product is without any side effects, and the patient takes like a shot.
Claims (4)
1 one kinds of suppositorys that contain lactobacillus is characterized in that the percentage by weight of forming this each component of suppository is: gelatin 10-40% glycerol 40-75% distilled water 10-30% lactobacillus culture fluid 5-30%.
2 suppositorys according to claim 1 is characterized in that the percentage by weight of forming this each component of suppository is: gelatin 27% glycerol 46% distilled water 18% lactobacillus culture fluid 9%.
3 according to claim 1 and 2 described suppositorys, it is characterized in that the lactobacillus of the contained effective ingredient of this suppository for living.
4 one kinds of preparation methoies that contain the suppository of lactobacillus is characterized in that the preparation method of this suppository is:
The preparation of lactobacillus liquid: the lyophilizing lactobacillus strain so that sterilization platinum earrings is gone bail for and deposited, change in the sterilized milk acidfast bacilli culture medium, cultivated 24 hours for 37 ℃, through a few hours inoculation, after smear for microscopic examination is qualified, it is inoculated in the lactobacillus culture fluid of sterilization, 37 ℃ of cultivations got final product in 24 hours;
Suppository preparation: take by weighing the gelatin 10-40% in the percentage by weight, soak, make it the deliquescing of expanding with distilled water 10-30%, add glycerol 40-75% again, make gelatin, glycerol dissolving in the water-bath evenly, place in the 100-126 ℃ of pressure cooker, pressure sterilizing 10-30 minute, add lactobacillus liquid 5-30% when temperature is reduced to 50-55 ℃, make mixing, inject the vaginal suppository mould, in the refrigerator internal cooling, coagulation forming takes out packing, gets product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96117361A CN1062450C (en) | 1996-12-05 | 1996-12-05 | Formula and preparation process of lactobacillaceau embolus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96117361A CN1062450C (en) | 1996-12-05 | 1996-12-05 | Formula and preparation process of lactobacillaceau embolus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1183965A CN1183965A (en) | 1998-06-10 |
| CN1062450C true CN1062450C (en) | 2001-02-28 |
Family
ID=5124240
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96117361A Expired - Fee Related CN1062450C (en) | 1996-12-05 | 1996-12-05 | Formula and preparation process of lactobacillaceau embolus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1062450C (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106389481A (en) * | 2016-08-31 | 2017-02-15 | 哈尔滨欧替药业有限公司 | Lactobacillus expansible vaginal suppository as well as preparation method and detection method thereof |
| CN111728937B (en) * | 2020-07-21 | 2022-09-06 | 华熙生物科技股份有限公司 | Private protective suppository filling containing zinc hyaluronate and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1057191A (en) * | 1991-06-29 | 1991-12-25 | 河南医科大学 | Nourishing skin cream |
| CN1105544A (en) * | 1994-09-09 | 1995-07-26 | 扬州大学农学院 | Method for preparing active lactic acid bacteria oral liquor |
| CN1130469A (en) * | 1995-11-27 | 1996-09-11 | 重庆医科大学贝尔科技实业总公司 | Double-fork milk beverage and preparation method thereof |
-
1996
- 1996-12-05 CN CN96117361A patent/CN1062450C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1057191A (en) * | 1991-06-29 | 1991-12-25 | 河南医科大学 | Nourishing skin cream |
| CN1105544A (en) * | 1994-09-09 | 1995-07-26 | 扬州大学农学院 | Method for preparing active lactic acid bacteria oral liquor |
| CN1130469A (en) * | 1995-11-27 | 1996-09-11 | 重庆医科大学贝尔科技实业总公司 | Double-fork milk beverage and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1183965A (en) | 1998-06-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |