CN106234366A - A kind of initiation reagent of tobacco seed - Google Patents
A kind of initiation reagent of tobacco seed Download PDFInfo
- Publication number
- CN106234366A CN106234366A CN201610600691.8A CN201610600691A CN106234366A CN 106234366 A CN106234366 A CN 106234366A CN 201610600691 A CN201610600691 A CN 201610600691A CN 106234366 A CN106234366 A CN 106234366A
- Authority
- CN
- China
- Prior art keywords
- seed
- iaa
- reagent
- tobacco seed
- tobacco
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000208125 Nicotiana Species 0.000 title claims abstract description 14
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 14
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 14
- 230000000977 initiatory effect Effects 0.000 title claims abstract description 14
- 229930191978 Gibberellin Natural products 0.000 claims abstract description 9
- 239000003448 gibberellin Substances 0.000 claims abstract description 9
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 claims abstract description 9
- 229930192334 Auxin Natural products 0.000 claims abstract description 3
- 239000002363 auxin Substances 0.000 claims abstract description 3
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 238000005213 imbibition Methods 0.000 claims 1
- 230000037452 priming Effects 0.000 abstract description 14
- 239000003999 initiator Substances 0.000 abstract description 6
- 238000005286 illumination Methods 0.000 description 5
- 230000009977 dual effect Effects 0.000 description 3
- 230000035784 germination Effects 0.000 description 3
- 230000007226 seed germination Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000007405 data analysis Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses the initiation reagent of a kind of tobacco seed, it is characterised in that: both gibberellins GA3 and auxin heteroauxing IAA, IAA and GA3 mass ratio is 1:1 ~ 1:10, and GA3 be 10 ~ 100ppm, IAA be 1 ~ 10ppm.After the initiator using the present invention causes, compared with non-Priming Seeds, regularity and concordance that seed base-root is prominent improve.Compared with gibberellins Priming Seeds, the radicle elongation growth ability after highlighting strengthens.
Description
Technical field
The present invention relates to the initiation reagent of a kind of tobacco seed, belong to promotion seed germination field.
Background technology
Traditional method: 1, water causes, and temperature is 25 ± 2 DEG C, full exposure, and intensity of illumination is 1000lx, air flux
0.5m3/ h, water causes 32 ~ 38h；2, gibberellins causes, and temperature is 25 ± 2 DEG C, full exposure, and intensity of illumination is 1000lx, air
Flux 0.5m3/ h, 50mg/L gibberellins causes 20 ~ 24h；Advantage: compared with non-Priming Seeds, the regularity that seed base-root is prominent
Improve with concordance；Shortcoming: compared with non-Priming Seeds, the radicle elongation growth reduced capability after highlighting.
Summary of the invention
The technical problem to be solved in the present invention is: provide the initiation reagent of a kind of grass seed, to solve prior art existence
Problem.
The technical scheme is that the initiation reagent of a kind of tobacco seed, gibberellins GA3 and auxin heteroauxing
Both IAA, IAA and GA3 mass ratio is 1:1 ~ 1:10, and GA3 be 10 ~ 100ppm, IAA be 1 ~ 10ppm.
Described ratio optimum is 1:1.
The initiating method causing reagent of a kind of tobacco seed, temperature is 26 ± 1 DEG C, and reagent will be caused the most molten
In Xie Shui, after being completely dissolved, being dipped in initiation reagent solution by weight 1:2.5 ~ 1:5 by tobacco seed, seed water suction is swollen
Swollen 24 ~ 48h, sucting expansion seed at 38 DEG C to do next time, Hui Gan to seed moisture content at 7-8%.
Beneficial effects of the present invention: after using the initiator of the present invention to cause, compared with non-Priming Seeds, seed base-root is dashed forward
Go out and just reached more than 90% at 108 hours, and non-Priming Seeds is less than 50%.Compared with gibberellins Priming Seeds, the present invention's
Radicle length after initiator is prominent after causing reaches 2.7cm, and gibberellins causes only 1.7cm, illustrates that this initiator causes
After process, seed base-root elongation growth ability strengthens.
Detailed description of the invention
Embodiment 1
1 seed priming processes
Tobacco seed is processed seed soaking 24h:CK1(water following respectively), CK2, GA3(100mg/L), GA3(100mg/L)+
IAA(10mg/L), totally 4 process, often process 100, repeat for 3 times.
Potentiality of seed and the mensuration of germination percentage
100, representational seed selected by each culture dish, and seed spacing is 5 ~ 8 times of its length.Use growth cabinet,
Temperature controls at 20 ~ 25 DEG C, and relative humidity controls 80% ~ 90%, cultivates, instill 3 ~ 4 dual distillations every day under illumination condition
Water, after 7d calculate germinating energy, after 14d calculate germination percentage, every 8h several once.
The mensuration of seed biological yield
Weigh after tobacco seed is positioned over papery culture bed germination 156h.
The mensuration of seed vitality
Each process selected seed 0.5g, cleans the surface of the seed with dual distilled water, and sterilizing absorbent filter blots surface and swims, puts
Enter in 100 mL beakers, add the 50 dual distilled water of mL, under 10 DEG C of constant temperature, soak 18 h, then with DDS307+ type conductance
Instrument measures soak electrical conductivity；Boiling water bath 30 min again；After cooling, measure absolute electrical conductivity, and calculate relative conductivity, relatively
Electrical conductivity=soak electrical conductivity/absolute electrical conductivity.Every kind repeats for 3 times, calculates meansigma methods.
Data analysis
Use WPS 2010 to carry out Data Analysis Services, then use SPSS16.0 to carry out elementary statistics calculating correction values and dependency
Analyze.
Table 1 is the prominent regularity of seed base-root and concordance
Table 2 is weight (repeating for the 4 times) example 1 sprouted and often process every 10 radicles after 156
Embodiment 2
Seed priming
The seed in river, flue-cured tobacco cultivars south 3 is provided by Guizhou Tobacco academy of science.Experiment set 3 process: CK1(does not causes), CK2
(water initiation) and GA3Cause.At 100mg*L after the surface of the seed sterilization-1 GA3Solution or ddH2O causes 24 hours.Seed priming
Condition is that 26 ° of C 12 h illumination/12 h are dark.Then ddH is used2O cleans seed 3 times, GA3And ddH2The seed that O caused exists
Being dried at 26 DEG C, seed moisture content reaches about 10% stopping and being dried (aborning, seed moisture content enough satisfied bag when 10%
Clothing).
Seed germination and vitality test
3 process, CK1 (non-Priming Seeds), CK2 (water Priming Seeds) and GA3Priming Seeds carries out sprouting experiment.Each process
If 4 times are repeated, 100 uniform program requests of seed of each repetition are in covering the plastic culture dish of 90 mm dias of double-layer filter paper.
Cultivate under the conditions of 26 DEG C of 12h illumination/12h dark photoperiod.The criterion of seed germination is visible radicle length and kind
Son is isometric.After 156h, each process randomly selects ten seeds sprouted, and excises radicle with blade, then by 10-4g
The weight of balance measurement radicle, uses WinRHIZO image scanning system to analyze radicle length and radicle surface area.
Table 3 is the character size example 2 sprouted and often process radicle after 156
Conclusion: understood by table 1,2 and 3, beneficial effects of the present invention: use the initiator GA of the present invention3(100mg/l)/IAA
(100mg/L) after causing, compared with non-Priming Seeds, regularity and concordance that seed base-root is prominent are aobvious when 108 hours
Write and improve.Compared with gibberellins Priming Seeds, the long 1cm of radicle length after highlighting, the initiator GA of the present invention is described3
(100mg/l) after/IAA (100mg/L) causes, seed base-root elongation growth ability strengthens.
Claims (3)
1. the initiation reagent of a tobacco seed, it is characterised in that: gibberellins GA3With auxin heteroauxing IAA, IAA and GA3
The two mass ratio is 1:1 ~ 1:10, and GA3Be 10 ~ 100ppm, IAA be 1 ~ 10ppm.
The initiation reagent of a kind of tobacco seed the most according to claim 1, it is characterised in that: described ratio optimum is 1:
1。
The initiating method causing reagent of a kind of tobacco seed the most as claimed in claim 1 or 2, it is characterised in that: temperature is
26 ± 1 DEG C, initiation reagent is dissolved in water according to the above ratio, after being completely dissolved, by tobacco seed by weight 1:2.5 ~ 1:5
Being dipped in initiation reagent solution, seed imbibition 24 ~ 48h, sucting expansion seed is at 38 DEG C to do next time, and Hui Gan is to seed moisture content
At 7-8%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610600691.8A CN106234366A (en) | 2016-07-28 | 2016-07-28 | A kind of initiation reagent of tobacco seed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610600691.8A CN106234366A (en) | 2016-07-28 | 2016-07-28 | A kind of initiation reagent of tobacco seed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106234366A true CN106234366A (en) | 2016-12-21 |
Family
ID=57603942
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610600691.8A Pending CN106234366A (en) | 2016-07-28 | 2016-07-28 | A kind of initiation reagent of tobacco seed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106234366A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109006822A (en) * | 2018-09-11 | 2018-12-18 | 河南农业大学 | A kind of azole pesticide seed coating that prevents is to the composition of wheat and corn side effect |
| CN115474457A (en) * | 2022-09-12 | 2022-12-16 | 贵州省烟草科学研究院 | Method for breaking dormancy of tobacco pelleted seeds |
| CN115735925A (en) * | 2022-11-28 | 2023-03-07 | 福建农林大学 | Fujian cypress seed initiator and using method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101411329A (en) * | 2008-12-04 | 2009-04-22 | 湖南省烟草公司永州市公司 | Initiating agent of tobacco seeds |
| CN102415236A (en) * | 2011-08-19 | 2012-04-18 | 青岛中烟种子有限责任公司 | Complete nutrition formula germination accelerating method for tobacco seed |
| CN105052300A (en) * | 2015-08-26 | 2015-11-18 | 许五妮 | Method for increasing germination rate of pumpkin seeds |
-
2016
- 2016-07-28 CN CN201610600691.8A patent/CN106234366A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101411329A (en) * | 2008-12-04 | 2009-04-22 | 湖南省烟草公司永州市公司 | Initiating agent of tobacco seeds |
| CN102415236A (en) * | 2011-08-19 | 2012-04-18 | 青岛中烟种子有限责任公司 | Complete nutrition formula germination accelerating method for tobacco seed |
| CN105052300A (en) * | 2015-08-26 | 2015-11-18 | 许五妮 | Method for increasing germination rate of pumpkin seeds |
Non-Patent Citations (2)
| Title |
|---|
| 招启柏等: "光、温、激素对烟草种子萌发和幼苗生长的影响", 《中国烟草学报》 * |
| 李振华: "烟草种子萌发的激素效应研究进展", 《云南农业大学学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109006822A (en) * | 2018-09-11 | 2018-12-18 | 河南农业大学 | A kind of azole pesticide seed coating that prevents is to the composition of wheat and corn side effect |
| CN115474457A (en) * | 2022-09-12 | 2022-12-16 | 贵州省烟草科学研究院 | Method for breaking dormancy of tobacco pelleted seeds |
| CN115474457B (en) * | 2022-09-12 | 2024-05-14 | 贵州省烟草科学研究院 | Method for breaking dormancy of tobacco pelleted seeds |
| CN115735925A (en) * | 2022-11-28 | 2023-03-07 | 福建农林大学 | Fujian cypress seed initiator and using method thereof |
| CN115735925B (en) * | 2022-11-28 | 2024-04-26 | 福建农林大学 | Fujian cypress seed initiator and application method thereof |
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| PB01 | Publication | ||
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Application publication date: 20161221 |