CN105935054B - Use of triterpenes for improving algal bloom - Google Patents

Abstract

Use of a triterpenoid for ameliorating algal blooms, the triterpenoid being administered to a body of fresh water to inhibit hyperproliferation of algal cells in the body of fresh water; wherein the triterpenoid is selected from dehydro-poric acid or dehydrothiochromic acid.

Description

Use of triterpenoids to improve algal blooms

technical field

The invention relates to the use of a triterpenoid compound, in particular to the use of a triterpenoid compound for improving algal blooms.

Background technique

Algal bloom, also known as water eutrophication, is a phenomenon in which the nitrogen and phosphorus content in fresh water is too high, exceeding the environmental capacity and self-purification capacity, resulting in the excessive proliferation of algae, which may cause pollution of drinking water sources. Hazard to human health.

Contents of the invention

The invention provides an application of a triterpenoid compound for improving algal bloom phenomenon, and the triterpenoid compound is used as an active ingredient for inhibiting excessive proliferation of algae cells.

The use of the triterpenoid compound described in the present invention to improve algal blooms, the triterpenoid compound is thrown into a fresh water body to inhibit the excessive proliferation of algae cells in the fresh water body; wherein, the triterpenoid compound is selected from Dehydroperoric acid or dehydrothiaroporic acid.

The use of the triterpenoid compound of the present invention for improving algal blooms, wherein the triterpenoid compound is dehydrodental acid, and the dosage is 30-500 μg/mL once a month Throw in the freshwater body.

The use of the triterpenoid compound described in the present invention to improve algal blooms, wherein the triterpenoid compound is deshydrothioxoporic acid, and the dosage is 30-500 μg/mL once a month Give the dose to the fresh water body.

The use of the triterpenoid compound of the present invention to improve the phenomenon of algal bloom, with the help of the triterpenoid compound as the active ingredient used to inhibit the excessive proliferation of algal cells, to prevent the pollution of drinking water sources caused by the phenomenon of algal bloom, to achieve The effect of maintaining human health.

Description of drawings

Figure 1a: is the chemical structural formula of the triterpenoid dehydrohaloforic acid.

Figure 1b: The chemical structural formula of the triterpenoid thiazochromic acid.

Figure 2a: Total ion chromatography results of triterpenoids.

Figure 2b: Selected ion chromatography results for triterpenoids.

Fig. 3a: the effect of the triterpenoid dehydrohaloforic acid on the survival rate of algae cells (trend line formula: y=84.041*e^(-0.0052947x), R ² =0.95112).

Figure 3b: the effect of triterpenoid thiazochromic acid on the survival rate of algal cells (trend line formula: y=74.777*e^(-0.0071371x), R ² =0.88586).

Detailed ways

In order to make the above and other purposes, features and advantages of the present invention more comprehensible, the preferred embodiments of the present invention are specifically cited below, together with the accompanying drawings, as follows:

The "fresh water body" in the present invention generally refers to various places where water accumulates, which can be natural water bodies such as rivers, lakes, ponds, streams, etc., and also includes artificial water bodies such as reservoirs and harbors.

The triterpenoid compound of the present invention can be thrown into a fresh water body to inhibit the excessive proliferation of algae cells in the fresh water body, wherein the triterpenoid compound can be dehydroeburicoic acid (dehydroeburicoic acid, whose chemical structural formula is as follows: As shown in Figure 1a), dehydrosulfurenic acid (dehydrosulfurenic acid, its chemical structural formula is shown in Figure 1b). The aforementioned triterpenoids may be artificially synthesized compounds, or natural compounds extracted from Chinese herbal medicines such as Antrodia camphorata, without limitation here.

It should be noted that the dosage and frequency of use of the triterpenoids may vary depending on whether the algal bloom is formed or not. For example: when the algal bloom has not yet formed in the fresh water body, the administration of the triterpenoid compound can be used to prevent the algal bloom phenomenon in the fresh water body. At this time, the frequency of use of the triterpenoid compound can be Once a month, and the dosage can be 30-200 μg/mL (dehydrodental acid, dehydrothiochromic acid); or, after algal blooms have formed in the fresh water body, you can use this The administration of triterpenoids promotes the death of excessively proliferating algae cells, so as to eliminate the algal blooms in the fresh water body. It is administered for at least 3 months, and the dose of administration may be 200 to 500 μg/mL (dehydroceriferic acid, thiazochromic acid).

In order to confirm that the triterpenoids of the present invention can indeed inhibit the growth of algae cells, and then can be used as active ingredients for improving algal blooms, the following tests were carried out:

(A) Extraction and purification of triterpenoids

In this test, a sample of Antrodia cinnamomea was taken, first crushed to a particle size of about 100nm, and then extracted with an aqueous ethanol solution (95% concentration by volume) for 2 hours (1 to 3 liters of Antrodia cinnamomea sample per kilogram) ethanol aqueous solution for extraction), followed by concentration under reduced pressure to obtain a primary extract.

Continue to carry out the liquid phase-liquid phase distribution extraction of the initial extract, dissolve the initial extract in pure water, add an equal volume of n-hexane, shake and mix evenly, and let it stand until it is completely separated to obtain a water layer solution, the aqueous layer solution was mixed with equal volumes of ethyl acetate, after uniform shaking and mixing, it was left to stand until it was completely separated to obtain an ethyl acetate layer solution, and the ethyl acetate layer solution was concentrated under reduced pressure, to obtain an ethyl acetate layer extract.

After back-dissolving the ethyl acetate layer extract with ethyl acetate, adsorb on silica gel particles, fill the silica gel particles into a column, use a mixture of water and methyl cyanide as the mobile phase, and use a flow rate of 2 mL/min Eluting is carried out to obtain several eluents containing dehydroceridic acid dehydrothiochromic acid respectively, and then the several eluents are washed and crystallized or recrystallized to obtain ultra-high purity dehydrogenated Dental acid and desulphuroporic acid.

Next, the results of total ion chromatography (TIC) and selective ion chromatography (selective ion monitoring, SIM) were carried out on the dehydroceratic acid and dehydrothioxoporic acid obtained through the aforementioned process, respectively. As shown in Figures 2a and 2b, the mass-to-charge ratio (m/z) and retention time (RT for short) of dehydrohaloforic acid and dehydrothioporoic acid are recorded in Table 1, respectively.

Table 1, the mass-to-charge ratio and residence time of each triterpenoid compound in this test.

Triterpenes m/z[M+H] ⁺ RT(min) dehydrohalogenate 469.55 19.89 Dehydrothiazotoporic acid 485.4 9.06

Accordingly, two kinds of triterpenoids, such as dehydrohaloforic acid and dehydrosulfanic poroporic acid, can be extracted and purified from the Antrodia camphorata sample.

(B) The effect of triterpenoids on the survival rate of algae cells

In this experiment, the algae cells of Microzystis aeruginosa were tested. It is an algae produced in freshwater and has toxicity. Therefore, after excessive proliferation and formation of algal blooms, it may endanger human health.

Add different concentrations of dehydrohaloforic acid and dehydrothiochromic acid to the algal cells that have been cultured to the stationary phase, and observe the survival rate of the algal cells after 72 hours by flow cytometry. The results are shown in Figure 3a- 3b.

According to the results shown in Figures 3a to 3b, it can be known that dehydroceridic acid and dehydrothioporic acid can indeed inhibit the growth of algae cells, and it can be known from calculations that dehydroceridic acid, dehydroporate The IC ₅₀ values of thioporoic acid were 98 μg/mL and 94 μg/mL, respectively.

Based on the above, the use of the triterpenoids of the present invention to improve algal blooms, by using the triterpenoids as an active ingredient for inhibiting the excessive proliferation of algal cells, prevents the pollution of drinking water sources caused by algal blooms problem, to achieve the effect of maintaining human health.

The above are only preferred embodiments of the present invention, and should not be used to limit the protection scope of the present invention. Therefore, all simple equivalent changes and modifications made with reference to the description of the present invention still belong to the protection scope of the present invention.

Claims (3)

1. a kind of triterpene compound is improving the purposes of algal tufa phenomenon, which is characterized in that the triterpene compound is thrown in one A poisons in freshwater, to inhibit the hyper-proliferative of frustule in the poisons in freshwater；

Wherein, which is selected from dehydroeburicoic acid or dehydrosulphurenic acid.

2. triterpene compound as described in claim 1 is improving the purposes of algal tufa phenomenon, which is characterized in that wherein, should Triterpene compound is dehydroeburicoic acid, and with monthly, the administering dosage of 30~500 μ g/mL administers the fresh water every time Water body.

3. triterpene compound as described in claim 1 is improving the purposes of algal tufa phenomenon, which is characterized in that wherein, should Triterpene compound is dehydrosulphurenic acid, and with monthly, the administering dosage of 30~500 μ g/mL administers every time The poisons in freshwater.