CN105935054A - Use of triterpenes for improving algal bloom - Google Patents

Abstract

The use of a triterpenoid compound to improve the algal bloom phenomenon. The triterpenoid compound is added to a fresh water body to inhibit the excessive proliferation of algal cells in the fresh water body; wherein the triterpenoid compound is selected from the group consisting of dehydrogenated teeth. poric acid, desulfochromic polypore acid or 1,4-dimethyl-2,3-methylenedioxy-5-ethylbenzene.

Description

Use of triterpenoids to improve algal blooms

technical field

The invention relates to the use of a triterpenoid compound, in particular to the use of a triterpenoid compound for improving algal blooms.

Background technique

Algal bloom, also known as water eutrophication, is a phenomenon in which the nitrogen and phosphorus content in fresh water is too high, exceeding the environmental capacity and self-purification capacity, resulting in the excessive proliferation of algae, which may cause pollution of drinking water sources. Hazard to human health.

Contents of the invention

The invention provides an application of a triterpenoid compound for improving algal bloom phenomenon, and the triterpenoid compound is used as an active ingredient for inhibiting excessive proliferation of algae cells.

The use of the triterpenoid compound described in the present invention to improve algal blooms, the triterpenoid compound is thrown into a fresh water body to inhibit the excessive proliferation of algae cells in the fresh water body; wherein, the triterpenoid compound is selected from Dehydrohaloforic acid, dehydrothoroporic acid or 1,4-dimethyl-2,3-methylenedioxy-5-ethylbenzene.

The use of the triterpenoid compound of the present invention for improving algal blooms, wherein the triterpenoid compound is dehydrohaloforic acid, and is administered once a month at 30-500 μg/mL each time The dose is administered to the freshwater body.

The use of the triterpenoids described in the present invention to improve algal blooms, wherein the triterpenoids are deshydrothioxoporic acid, and the dosage is 30-500 μg/mL once a month The dose is administered to the fresh water body.

The use of the triterpenoids described in the present invention to improve algal blooms, wherein the triterpenoids are 1,4-dimethyl-2,3-methylenedioxy-5-ethylbenzene, and Once a month, the dose of 10-500 μg/mL is administered to the fresh water body.

The use of the triterpenoid compound of the present invention to improve the phenomenon of algal bloom, with the help of the triterpenoid compound as the active ingredient used to inhibit the excessive proliferation of algal cells, to prevent the pollution of drinking water sources caused by the phenomenon of algal bloom, to achieve The effect of maintaining human health.

Description of drawings

Figure 1a: is the chemical structural formula of the triterpenoid dehydrohaloforic acid.

Figure 1b: The chemical structural formula of the triterpenoid thiazochromic acid.

Figure 1c: the chemical structural formula of the triterpenoid 1,4-dimethyl-2,3-methylenedioxy-5-ethylbenzene.

Figure 2a: Total ion chromatography results of triterpenoids.

Figure 2b: Selected ion chromatography results for triterpenoids.

Figure 3a: The effect of the triterpenoid dehydrohaloforic acid on the survival rate of algae cells (trend line formula: y=84.041*e^(-0.0052947x), R ² =0.95112).

Figure 3b: The effect of triterpenoid thiazochromic acid on the viability of algal cells (trend line formula: y=74.777*e^(-0.0071371x), R ² =0.88586).

Figure 3c: The effect of triterpenoid 1,4-dimethyl-2,3-methylenedioxy-5-ethylbenzene on the survival rate of algal cells (trend line formula: y=62.763*e^(- 0.0072728x), R ² =0.83052).

detailed description

In order to make the above and other purposes, features and advantages of the present invention more comprehensible, the preferred embodiments of the present invention are specifically cited below, together with the accompanying drawings, as follows:

The "fresh water body" in the present invention generally refers to various places where water accumulates, which can be natural water bodies such as rivers, lakes, ponds, streams, etc., and also includes artificial water bodies such as reservoirs and harbors.

The triterpenoid compound of the present invention can be thrown into a fresh water body to inhibit the excessive proliferation of algal cells in the fresh water body, wherein the triterpenoid compound can be dehydroeburicoic acid (dehydroeburicoic acid, whose chemical structural formula is as follows: As shown in Figure 1a), dehydrosulfurenic acid (dehydrosulfurenic acid, whose chemical structure is shown in Figure 1b), 1,4-dimethyl-2,3-methylenedioxy-5-ethylbenzene ( 4,7-dimethoxy-5-methyl-1,3-benzodioxole, whose chemical structure is shown in Figure 1c). The aforementioned triterpenoids may be artificially synthesized compounds, or natural compounds extracted from Chinese herbal medicines such as Antrodia camphorata, without limitation here.

It should be noted that the dosage and frequency of use of the triterpenoids may vary depending on whether the algal bloom is formed or not. For example: when the algal bloom has not yet formed in the fresh water body, the administration of the triterpenoid compound can be used to prevent the algal bloom phenomenon in the fresh water body. At this time, the frequency of use of the triterpenoid compound can be Once a month, and the dosage can be 30-200 μg/mL (dehydrodental acid, dehydrothiochromic acid) or 10-200 μg/mL (1,4-dimethyl-2,3 -methylenedioxy-5-ethylbenzene); or, after algal blooms have formed in the fresh water body, the administration of the triterpenoids can be used to promote the death of excessively proliferating algal cells to To remove the algal bloom phenomenon in the fresh water body, at this time, the frequency of use of the triterpenoid compound can be 1-2 times a month, and it can be administered continuously for at least 3 months, and the dosage can be 200-500 μg/ mL (dehydrohaloforic acid, dehydrothioxoporic acid, or 1,4-dimethyl-2,3-methylenedioxy-5-ethylbenzene).

In order to confirm that the triterpenoids of the present invention can indeed inhibit the growth of algae cells, and then can be used as active ingredients for improving algal blooms, the following tests were carried out:

( A ) Extraction and purification of triterpenoids

In this experiment, a sample of Antrodia cinnamomea was taken, first crushed to a particle size of about 100 nm, and then extracted with an ethanol aqueous solution (95% concentration by volume) for 2 hours (1 to 3 liters per kilogram of Antrodia cinnamomea sample ethanol aqueous solution for extraction), followed by concentration under reduced pressure to obtain a primary extract.

Continue to carry out the liquid phase-liquid phase distribution extraction of the initial extract, dissolve the initial extract in pure water, add an equal volume of n-hexane, shake and mix evenly, and let it stand until it is completely separated to obtain a water layer solution, the aqueous layer solution was mixed with equal volumes of ethyl acetate, after uniform shaking and mixing, it was left to stand until it was completely separated to obtain an ethyl acetate layer solution, and the ethyl acetate layer solution was concentrated under reduced pressure, to obtain an ethyl acetate layer extract.

After redissolving the ethyl acetate layer extract with ethyl acetate, adsorb on silica gel particles, fill the silica gel particles into a column, use a mixture of water and cyanogen as the mobile phase, and use 2 mL/min The flow rate was eluted to obtain several elutions containing dehydrohaloforic acid, dehydrothoroporic acid, and 1,4-dimethyl-2,3-methylenedioxy-5-ethylbenzene, respectively. liquid, and then carry out washing crystallization or recrystallization of several eluents to obtain ultra-high purity dehydrohaloforic acid, dehydrothioxoporic acid and 1,4-dimethyl-2,3-methanoic acid Methylenedioxy-5-ethylbenzene.

Continue to carry out total ion chromatography ( total ion The results of chromatogram (TIC for short) and selective ion monitoring (SIM for short) are shown in Fig. The mass-to-charge ratio (m/z) and retention time (RT for short) of 2,3-methylenedioxy-5-ethylbenzene are recorded in Table 1, respectively.

Table 1, the mass-to-charge ratio and residence time of each triterpenoid compound in this test.

Triterpenes m/z [M+H] ⁺ RT (minutes) dehydrohalogenate 469.55 19.89 Dehydrothiazotoporic acid 485.4 9.06 1,4-Dimethyl-2,3-methylenedioxy-5-ethylbenzene 261.1 7.23

According to this, it is possible to extract and purify the trihydroporectic acid, dehydrothoroporic acid and 1,4-dimethyl-2,3-methylenedioxy-5-ethylbenzene from the Antrodia camphorata sample. a triterpenoid compound.

( B ) Effects of triterpenoids on the survival rate of algae cells

In this experiment, the algae cells of Microzystis aeruginosa were tested. It is a freshwater algae that produces toxicity. Therefore, after excessive proliferation and formation of algal blooms, it may endanger human health.

Add different concentrations of dehydrohaloforic acid, dehydrothioxoporic acid, and 1,4-dimethyl-2,3-methylenedioxy-5-ethylbenzene to the algae cells that have been cultured to the stationary phase In , the survival rate of algae cells after 72 hours was observed by flow cytometry, and the results are shown in Figures 3a-3c.

According to the results shown in Figures 3a to 3c, it can be known that dehydrohaloforic acid, dehydrothioxoporic acid, and 1,4-dimethyl-2,3-methylenedioxy-5-ethylbenzene are indeed Both can inhibit the growth of algae cells, and it can be known by calculation that dehydrohaloforic acid, dehydrothiochromoporic acid, 1,4-dimethyl-2,3-methylenedioxy-5-ethane The IC ₅₀ values of benzene are 98 μg/mL, 94 μg/mL and 31 μg/mL, respectively.

Based on the above, the use of the triterpenoids of the present invention to improve algal blooms, by using the triterpenoids as an active ingredient for inhibiting the excessive proliferation of algal cells, prevents the pollution of drinking water sources caused by algal blooms problem, to achieve the effect of maintaining human health.

The above are only preferred embodiments of the present invention, and should not be used to limit the protection scope of the present invention. Therefore, all simple equivalent changes and modifications made with reference to the description of the present invention still belong to the protection scope of the present invention.

Claims (4)

1. a triterpenoid compound is in order to improve the purposes of algal tufa phenomenon, it is characterised in that this triterpenoid compound is thrown in a poisons in freshwater, to suppress the hyper-proliferative of frustule in this poisons in freshwater； Wherein, this triterpenoid compound is selected from dehydroeburicoic acid, dehydrosulphurenic acid or Isosorbide-5-Nitrae-dimethyl-2,3-methylene-dioxy-5-ethylbenzene.

2. triterpenoid compound as claimed in claim 1 is in order to improve the purposes of algal tufa phenomenon, it is characterised in that wherein, this triterpenoid compound is dehydroeburicoic acid, and with monthly, the dosage that casts of each 30～500 μ g/mL casts this poisons in freshwater.

3. triterpenoid compound as claimed in claim 1 is in order to improve the purposes of algal tufa phenomenon, it is characterized in that, wherein, this triterpenoid compound is dehydrosulphurenic acid, and with monthly, the dosage that casts of each 30～500 μ g/mL casts this poisons in freshwater.

4. triterpenoid compound as claimed in claim 1 is in order to improve the purposes of algal tufa phenomenon, it is characterised in that wherein, this triterpenoid compound is Isosorbide-5-Nitrae-dimethyl-2,3-methylene-dioxy-5-ethylbenzene, and with monthly, the dosage that casts of each 10～500 μ g/mL casts this poisons in freshwater.