CN105920067A - Sunflower calathide extract containing polysaccharide, flavonoid and alkaloid and preparation method of sunflower calathide extract - Google Patents
Sunflower calathide extract containing polysaccharide, flavonoid and alkaloid and preparation method of sunflower calathide extract Download PDFInfo
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- CN105920067A CN105920067A CN201610294519.4A CN201610294519A CN105920067A CN 105920067 A CN105920067 A CN 105920067A CN 201610294519 A CN201610294519 A CN 201610294519A CN 105920067 A CN105920067 A CN 105920067A
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- sunflower disk
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- ethanol
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- 229930013930 alkaloid Natural products 0.000 title abstract description 5
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Veterinary Medicine (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a sunflower calathide extract containing polysaccharide, flavonoid and alkaloid and a preparation method of the sunflower calathide extract. Through scientific extraction, sunflower calathide, as a pure natural traditional Chinese medicine or a medicinal and edible material, can be prepared into functional health care medicines or medicinal and edible health care products for resisting gout, treating hyperuricemia, boosting immunity, reducing blood glucose and blood lipid, protecting kidney and liver, relieving swelling, diminishing inflammation and relieving pain, and the functional health care medicines or the medicinal and edible health care products have functions of dissolving uric acid calculus, reducing blood uric acid concentration, relieving swelling and resisting inflammation, regulating purine and uric acid metabolism, balancing acid and base in human body and balancing body's hormone; and the finished product is safe, free from side effects, obvious in health efficacy and good in prospect of market development, and the product can be taken for a long time.
Description
Technical field
The invention belongs to the field of Chinese medicines, be specifically related to a kind of sunflower disk extract, it comprises polysaccharide, general flavone and always biological
Alkali, additionally provides its preparation method simultaneously.
Background technology
Sunflower, as a kind of important oilseed plant, is extensively planted in worldwide.Sunflower disk is except sunflower seed
After sunflower plate, traditional application process be using except the sunflower disk after sunflower seed as animal feeding-stuff pasture or as burn
Material warming & cooking, has not given play to its bigger economic benefit and medical function.China's theory of traditional Chinese medical science is thought: floral disc of sunflower,
The property of medicine is sweet, cold, returns Liver Channel, the flat liver of function heat-clearing, analgesic hemostatic；Cure mainly high blood pressure, headache and dizzy, tinnitus, dysmenorrhoea, uterus go out
Blood, sore rash." Hebei Chinese herbal medicine " is said: " sunflower disk heat-clearing and damp-drying drug, dissipating bind of having a respite.Control headache, dizziness, acute toothache, hot eyes cloud
Screen, suffer from abdominal pain in throat, outward for cellulitis ", there is the effect of obvious anti-inflammatory, diuresis, step-down.China is that the plantation of sunflower is big
State, the aboundresources of sunflower disk, extracts from sunflower disk and prepares and have the composition of pharmacologically active and can not only provide and more face
Bed medication, and can also twice laid, turn waste into wealth, play extraordinary environmental-protection function, and medicine be pure natural source, dirty
Dye is few, and toxic and side effect is little.
Accordingly, it would be desirable to study the active component of sunflower disk further, to develop more using value.
Summary of the invention
Applying single, the problem of the wasting of resources to improve sunflower disk in prior art, the present invention is to the work in sunflower disk
Property composition done concrete research, and be surprised to find that extracting, through sunflower disk, the extract obtained has and preferably treat the skill of gout
Art effect.The present invention is achieved by the following technical solutions.
An object of the present invention is to provide a kind of sunflower disk extract, it is characterised in that obtained by sunflower disk alcohol extract
Arrive.In obtained sunflower disk extract, containing sunflower disk polysaccharide, sunflower disk general flavone and sunflower disk TA.
It has been found that sunflower disk alkaloid can form sunflower alkalinuria acid compound, compound with specific binding uric acid
Can excrete through liver metabolism.Suppress the chemotactic of inflammatory cell simultaneously and reduce inflammatory factor release, for acute attack
Gouty arthritis have uniqueness anti-inflammation detumescence effectiveness.Sunflower disk general flavone is the strongest a kind of antioxidant, can effectively remove
Internal oxygen radical, stops the oxidation of nucleic acid, plays the effect reducing purine content；Flavones can also reduce blood sugar and glycerine
Three esters, reduce capillary fragility, increase permeability and can alleviate kidney cell pressure, through more uric acid.Simultaneously can be steady
Determine kidney protein tubule, beneficially renal metabolism uric acid；Flavones can suppress oozing out of inflammatory biology enzyme, can be with pain relieving.Sunflower disk
Polysaccharide is that polysaccharide is a kind of, and polysaccharide is to be formed by the condensation of multiple monosaccharide molecule, dehydration, is the complicated and huge sugar of molecule structure
Class material.There is enhancing immune function of human body, tamm-Horsfall protein can be played regulating and controlling effect, also there is oxidation resistant effect,
Polysaccharide can promote regeneration and the healing of bone tissue, has hypoglycemic and blood fat.
In order to reach preferably to treat the effect of gout, in the sunflower disk extract of the present invention, containing of sunflower disk polysaccharide
Amount is with glucose meter as 42.9%-56.4%, and the content of sunflower disk general flavone is calculated as 58.3%-74.0% with rutin, and sunflower disk is always given birth to
The content of alkaloids is calculated as 72.0%-81.5% with methyl sunflower alkali.
Further, described sunflower disk extract is prepared by a method comprising the following steps and obtains:
1) being pulverized by sunflower disk, add the 50%-95% ethanol of 4-10 mass times, heating and refluxing extraction 1-3 time, each 1-3 is little
Time, merge extract, filter, 0.5-2 times of filtrate reduced in volume to quality of medicinal material, obtain alcohol extract；
2) alcohol extract of step 1) gained is filtered, take filtrate；
3) by step 2) filtrate of gained is after storng-acid cation exchange resin fully adsorbs, first with distilled water with 4-6BV/h
Flow velocity wash away water-solubility impurity, then elute with the flow velocity of 1-3BV/h with the 50%-95% ethanol of 5-10 mass times, collect
Eluent, reduced pressure concentration, it is dried, obtains sunflower disk general flavone；
4) by molten with the 50%-95% ethanol containing ammoniacal liquor 1%-5% for the storng-acid cation exchange resin that carries out in step 3) eluting
Liquid elutes with the flow velocity of 1-3BV/h, collects eluent, reduced pressure concentration, is dried, obtains sunflower disk TA；
5) being dried by the dregs of a decoction after step 1) alcohol extracting, after waving most ethanol, then by distilled water refluxing extraction 1-3 time, each 1-3 is little
Time, 4-10 times that quality is dregs of a decoction quality of distilled water the most used, merge extract, filter, filtrate reduced in volume is to medicinal material
0.5-2 times of quality, obtains decocting liquid；
6) by clarified dose of the decocting liquid process of step 5) gained, centrifugal, supernatant adds 95% ethanol of 3-5 mass times, stirs
Even, centrifugal, gained sediment is vacuum dried, and obtains sunflower disk polysaccharide；
7) by the sunflower disk general flavone of step 3) gained, the sunflower disk TA of step 4) gained and the certain herbaceous plants with big flowers of step 6) gained
Floral disc polysaccharide mixes, and obtains sunflower disk extract.
Wherein, described storng-acid cation exchange resin is preferably 732 types or HZ002 type, and described fining agent is preferred
For shitosan.
The sunflower disk extract of the present invention can be widely used in medicine and/or health products as active component.Therefore,
It is a further object to provide a kind of pharmaceutical composition, it is characterised in that containing above-mentioned sunflower disk extract with optional
The pharmaceutically acceptable auxiliary material in ground.The formulation of described pharmaceutical composition can be tablet, capsule, pill, granule, dissipate
Agent, micropill, solution, syrup, emulsion or injection.
On the other hand, the invention still further relates to the preparation method of described sunflower disk extract, it is characterised in that include following step
Rapid:
1) being pulverized by sunflower disk, add the 50%-95% ethanol of 4-10 mass times, heating and refluxing extraction 1-3 time, each 1-3 is little
Time, merge extract, filter, 0.5-2 times of filtrate reduced in volume to quality of medicinal material, obtain alcohol extract；
2) alcohol extract of step 1) gained is filtered, take filtrate；
3) by step 2) filtrate of gained is after storng-acid cation exchange resin fully adsorbs, first with distilled water with 4-6BV/h
Flow velocity wash away water-solubility impurity, then elute with the flow velocity of 1-3BV/h with the 50%-95% ethanol of 5-10 mass times, collect
Eluent, reduced pressure concentration, it is dried, obtains sunflower disk general flavone；
4) by molten with the 50%-95% ethanol containing ammoniacal liquor 1%-5% for the storng-acid cation exchange resin that carries out in step 3) eluting
Liquid elutes with the flow velocity of 1-3BV/h, collects eluent, reduced pressure concentration, is dried, obtains sunflower disk TA；
5) being dried by the dregs of a decoction after step 1) alcohol extracting, after waving most ethanol, then by distilled water refluxing extraction 1-3 time, each 1-3 is little
Time, 4-10 times that quality is dregs of a decoction quality of distilled water the most used, merge extract, filter, filtrate reduced in volume is to medicinal material
0.5-2 times of quality, obtains decocting liquid；
6) by clarified dose of the decocting liquid process of step 5) gained, centrifugal, supernatant adds 95% ethanol of 3-5 mass times, stirs
Even, centrifugal, gained sediment is vacuum dried, and obtains sunflower disk polysaccharide；
7) by the sunflower disk general flavone of step 3) gained, the sunflower disk TA of step 4) gained and the certain herbaceous plants with big flowers of step 6) gained
Floral disc polysaccharide mixes, and obtains sunflower disk extract.
Wherein, described storng-acid cation exchange resin is preferably 732 types or HZ002 type, and described fining agent is preferred
For shitosan.
The invention still further relates to described sunflower disk extract or pharmaceutical composition purposes in preparing anti-gout drugs.
The positive effect of the present invention is: utilize concrete preparation method extract from sunflower disk obtain containing sunflower disk many
The extract of the effective active compositions such as sugar, general flavone and TA product, this extract is natural origin, and toxic and side effect is little,
Security is high, and tests prove that have good result for the treatment of to gout, and clinical treatment effect is notable.Divide it addition, extract
Separating process is simple, it is easy to operation, it is not necessary to special installation, reagent environmentally safe with low cost, used, is suitable for extensive
Industrialized production.
Detailed description of the invention
Illustrated further the description present invention by following example, and limits the present invention never in any form, without departing substantially from
On the premise of the technical solution of the present invention, what those of ordinary skill in the art made for the present invention easily realized any changes
Within dynamic or change falls within scope of the presently claimed invention.
Embodiment 1
The preparation process of sunflower disk extract:
1), taking the 50% ethanol heating and refluxing extraction 1 time adding 4 mass multiples after sunflower disk 1kg pulverizes, each 1 hour, merging carried
Take liquid, filter, 0.5 times of filtrate reduced in volume to quality of medicinal material, obtain alcohol extract；
2), the alcohol extract of step 1) gained filter, take filtrate；
3), by step 2) filtrate of gained is after 732 type storng-acid cation exchange resins fully adsorb, first with distilled water with 4
The flow velocity of BV/h washes away water-solubility impurity, then elutes with the flow velocity of 1BV/h with 50%% ethanol of 5 mass multiples, and collection is washed
De-liquid, reduced pressure concentration, it is dried, obtains sunflower disk general flavone；Its content is calculated as 61.4% with rutin；
4), by the 732 type storng-acid cation exchange resins that carry out in step 3) eluting again with 50% ethanol containing ammoniacal liquor 5%
Solution elutes with the flow velocity of 1BV/h, collects eluent, reduced pressure concentration, is dried, obtains sunflower disk TA.Its content
In terms of methyl sunflower alkali, content is for 75.8%；
5), the dregs of a decoction after step 1) alcohol extracting are dried, after waving most ethanol, then by distilled water refluxing extraction 1 time, each 1 hour,
Quality is dregs of a decoction quality 4 times of distilled water the most used, merge extract, filter, and filtrate reduced in volume is to quality of medicinal material
0.5 times, obtain decocting liquid；
6), being processed through shitosan by the decocting liquid of step 5) gained, centrifugal, supernatant adds 95% ethanol of 3 mass times amount, stirs
Even, centrifugal, gained sediment is vacuum dried, and obtains sunflower disk polysaccharide；Recording its content by Anthrone-sulfuricacid method with glucose meter is
45.2%；
7) by the sunflower disk general flavone of step 3) gained, the sunflower disk TA of step 4) gained and the certain herbaceous plants with big flowers of step 6) gained
Floral disc polysaccharide mixes, and obtains sunflower disk extract.
Embodiment 2
The preparation process of sunflower disk extract:
1), take the 75% ethanol heating and refluxing extraction 2 times adding 6 mass multiples after sunflower disk 1kg pulverizes, each 2 hours, merge
Extract, filters, 1 times of filtrate reduced in volume to quality of medicinal material, obtains alcohol extract；
2), the alcohol extract of step 1) gained filter, take filtrate；
3), by step 2) filtrate of gained after HZ002 type storng-acid cation exchange resin fully adsorbs, first with distilled water with
The flow velocity of 5BV/h washes away water-solubility impurity, then elutes with the flow velocity of 2BV/h with 75% ethanol of 8 mass multiples, and collection is washed
De-liquid, reduced pressure concentration, it is dried, obtains sunflower disk general flavone；Its content is calculated as 66.4% with rutin；
4), by the HZ002 type storng-acid cation exchange resin that carries out in step 3) eluting again with the volume hundred containing ammoniacal liquor 3%
Point concentration is that 75% ethanol solution elutes with the flow velocity of 2BV/h, collects eluent, reduced pressure concentration, is dried, obtains sunflower
Dish TA, its content in terms of methyl sunflower alkali content for 76.8%；
5), the dregs of a decoction after step 1) alcohol extracting are dried, after waving most ethanol, then by distilled water refluxing extraction 2 times, each 2 hours, often
Quality is dregs of a decoction quality 8 times of secondary distilled water used, merge extract, filter, the 1 of filtrate reduced in volume to quality of medicinal material
Times, obtain decocting liquid；
6), being processed through shitosan by the decocting liquid of step 5) gained, centrifugal, supernatant adds 95% ethanol of 4 mass times amount, stirs
Even, centrifugal, gained sediment is vacuum dried, and obtains sunflower disk polysaccharide；Recording its content by Anthrone-sulfuricacid method with glucose meter is
49.8%；
7), by the sunflower disk general flavone of step 3) gained, the sunflower disk TA of step 4) gained and the certain herbaceous plants with big flowers of step 6) gained
Floral disc polysaccharide mixes, and obtains sunflower disk extract.
Embodiment 3
The preparation process of sunflower disk extract:
1), by sunflower disk 1kg pulverize the 75% ethanol heating and refluxing extraction 3 times adding 10 mass multiples, each 3 hours, merge and extract
Liquid, filters, 2 times of filtrate reduced in volume to quality of medicinal material, obtains alcohol extract；
2), the alcohol extract of step 1) gained filter, take filtrate；
3), by step 2) filtrate of gained after HZ002 type storng-acid cation exchange resin fully adsorbs, first with distilled water with
The flow velocity of 6BV/h washes away water-solubility impurity, then is that 95% ethanol elutes with the flow velocity of 3BV/h by 10 mass multiples, concentration,
Collect eluent, reduced pressure concentration, be dried, obtain sunflower disk general flavone；Its content is calculated as 70.5% with rutin；
4), by the HZ002 type storng-acid cation exchange resin that carries out in step 3) eluting again by 95% second containing ammoniacal liquor 5%
Alcoholic solution elutes with the flow velocity of 3BV/h, collects eluent, reduced pressure concentration, is dried, obtains sunflower disk TA, and it contains
Amount in terms of methyl sunflower alkali content for 77.6%；
5), the dregs of a decoction after step 1) alcohol extracting are dried, after waving most ethanol, then by distilled water refluxing extraction 3 times, each 3 hours,
Quality is dregs of a decoction quality 10 times of distilled water the most used, merge extract, filter, and filtrate reduced in volume is to quality of medicinal material
2 times, obtain decocting liquid；
6), being processed through shitosan by the decocting liquid of step 5) gained, centrifugal, supernatant adds 5 times amount 95% ethanol, stirs evenly, from
The heart, gained sediment is vacuum dried, obtains sunflower disk polysaccharide；Recording its content by Anthrone-sulfuricacid method with glucose meter is
53.7%；
7), by the sunflower disk general flavone of step 3) gained, the sunflower disk TA of step 4) gained and the certain herbaceous plants with big flowers of step 6) gained
Floral disc polysaccharide mixes, and obtains sunflower disk extract.
Test example 1: prove the pharmacological action for the treatment of gout with animal model
One, test material
1. animal: Kunming mouse, body weight (20g ± 2g), Jilin University's clinic Animal Research Center provide.
2. it is administered sample: blank group (distilled water), of the present invention group (sunflower disk that embodiment 1,2,3 prepares carries
Take thing)
Two, test method
1. analgesic experiment
Take male mice 40, be randomly divided into blank group, sunflower disk extract group (embodiment 1, embodiment 2, embodiment
3), often 10 are organized.By 120mg kg-1Being administered, 1 time one day, blank group gave equivalent distilled water, successive administration 5 days, Yu Mo
After secondary administration 1 hour, often organize and take 10 mouse peritoneals and inject 0.7% acetic acid 0.1ml g-1, turn round in recording each group of mouse 20min
Body number of times.
The impact of table 1. Dichlorodiphenyl Acetate induced mice writhing response
Compare with blank group, * P < 0.05, * * P < 0.01
2. Mouse Blood uric acid (UA), xanthine oxidase (XOD) and urea nitrogen (BUN)
Take male mice 100, be randomly divided into normal group, model group, sunflower disk extract group (embodiment 1, embodiment of the present invention
2, embodiment 3), often group 20.In addition to normal group, often group is all with yeast 30g kg-1Gavage, continuous one week.From the beginning of the 3rd day
Of the present invention group of gavage, by 120mg kg-1It is administered, successive administration 5d.Mouse is extractd eyeball method and takes blood, separates serum, at wavelength
UA, XOD and BUN value is measured at 690nm, 530nm and 640nm.
Table 2. causes the impact of hyperuricemia mice serum UA, XOD and BUN to yeast extract
* P < 0.05, * * P < 0.01 is compared with normal group;* P < 0.05, * * P < 0.01 n=20 is compared with model group
Three, result of the test
The result of the test of above-mentioned Tables 1 and 2 shows, the sunflower disk extract of the present invention can significantly alleviate the pain that chemical substance produces
Bitterly, illustrate that the sunflower disk extract of the present invention has analgesic activity.It addition, the sunflower disk extract of the present invention can significantly inhibit Huang
Purine oxidase activity, reduces the generation of uric acid, shows substantially reduce blood urea nitrogen, improve renal function.
Test example 2: typical clinical case
Case 1:
Lee, man, 48 years old, after once drinking, there is suddenly left instep before 6 years in patient, thumb swells and ache, it is difficult to fall asleep, office
The scorching hot redness in portion.After anti-inflammatory throe medicine is taken in locality, pain relief after a week.After, the most easily send out after drinking whenever or catching a cold
Make, show effect whenever, certainly take the medicines such as metacortandracin.Take medicine-feeding poor effect in this year.Pain is fixed on left instep and left thumb.
Treatment: after taking sunflower disk extract 2 course for the treatment of of the embodiment of the present invention 1 preparation, transference cure, walking freely, nothing
Walk lamely.Advise continue to take 3 courses for the treatment of for consolidating curative effect.Follow up a case by regular visits to half a year, do not recur.
Case 2:
Certain female, 67 years old, once had joint bone aching history also not take notice of, treating, over the past two years due to diet seafood, symptom adds
Weight, each joint of whole body is substantially all gets involved. and hospital diagnosis is urarthritis.
Treatment: after taking sunflower disk extract 1 course for the treatment of of the embodiment of the present invention 1 preparation, transference cure, normal waking activity does not has
Impact, indices is normal, and eating habit is corrected one's mistakes the most substantially, advises continue to take 3 courses for the treatment of for consolidating curative effect.Follow up a case by regular visits to 2
Year, do not recur.
Case 3:
Zhao, man, 59 years old, vexed because of continuous foot sole of the foot toe ache influence sleep at night, dry and hard excrement full with gastral cavity abdomen ruffian, look into
Dimly red tongue, tongue is the thickest, wiry and rolling pulse.Blood test is pointed out, Hyperlipidemia, blood uric acid 554umol/L.Patient's body is plentiful,
Work dinner party is many, lives irregular, and partial eclipse plumpness weight taste, addicted to drinking beer and cola drink.
Taking the sunflower disk extract of the embodiment of the present invention 1 preparation, taking pain i.e. has and alleviates, swelling pain after 2 courses for the treatment of
Pain substantially disappears and follows up a case by regular visits to 1 year, does not recurs.
Case 4:
Wang, 45 years old present illness history of man: started from 2013, suffer from gout 3 years, annual outbreak 3-4 time.High lithemia value
680umol/L, right foot part articulationes metatarsophalangeae obvious pain during outbreak, with red and swollen phenomenon.During hurt like hell, applied Diclofenac
Sodium pain relieving.Personal history: eating seafood, meat, edible egg, bean product are in the majority.Take situation: on December 29th, 2015, hospital
Internal jugular vein blood records uric acid level 517.37umol/L, creatinine: 101.69umol/L triglycerides: 1.89mmol/L phenylalanine ammonia turns
Change enzyme: 59.46IU/L.On February 21st, 2016, hospital recorded uric acid level 497.50umol/L.
The sunflower disk extract of the embodiment of the present invention 1 is started to take from January 4th, 2016, instructions of taking: 10g/ times/day,
Play administered at empty stomach morning to use.January 15, foot pain, pain degree patient can stand, and does not takes any analgesic drug product, base after three days
This improvement；February 29, recording finger tip blood 460umol/L, gout outbreak, because of working principle, takes C14H10Cl2NNaO2 during this period
Pain relieving, pain relief in 2 days；March 5, patient rivers bend pin, does not induces gout；19-20 day in March, there is pain in patient's left foot instep,
Pain degree patient is difficult to stand, because patient's stomach is uncomfortable, it is proposed that take Etoricoxib, but patient does not has medication all the time, 2-3 days
Remission.At present patient perceptions is good, still takes continuing.
Claims (10)
1. a sunflower disk extract, it is characterised in that obtained by sunflower disk alcohol extract.
2. sunflower disk extract as claimed in claim 1, it is characterised in that containing sunflower disk polysaccharide, sunflower disk general flavone and certain herbaceous plants with big flowers
Floral disc TA.
3. sunflower disk extract as claimed in claim 2, it is characterised in that the content of sunflower disk polysaccharide with glucose meter is
42.9%-56.4%, the content of sunflower disk general flavone is calculated as 58.3%-74.0% with rutin, and the content of sunflower disk TA is with first
Base sunflower alkali is calculated as 72.0%-81.5%.
4. the sunflower disk extract as described in one of claim 1-3, it is characterised in that the preparation method of described extract includes
Following steps:
1) being pulverized by sunflower disk, add the 50%-95% ethanol of 4-10 mass times, heating and refluxing extraction 1-3 time, each 1-3 is little
Time, merge extract, filter, 0.5-2 times of filtrate reduced in volume to quality of medicinal material, obtain alcohol extract；
2) alcohol extract of step 1) gained is filtered, take filtrate；
3) by step 2) filtrate of gained is after storng-acid cation exchange resin fully adsorbs, first with distilled water with 4-6BV/h
Flow velocity wash away water-solubility impurity, then elute with the flow velocity of 1-3BV/h with the 50%-95% ethanol of 5-10 mass times, collect
Eluent, reduced pressure concentration, it is dried, obtains sunflower disk general flavone；
4) by molten with the 50%-95% ethanol containing ammoniacal liquor 1%-5% for the storng-acid cation exchange resin that carries out in step 3) eluting
Liquid elutes with the flow velocity of 1-3BV/h, collects eluent, reduced pressure concentration, is dried, obtains sunflower disk TA；
5) being dried by the dregs of a decoction after step 1) alcohol extracting, after waving most ethanol, then by distilled water refluxing extraction 1-3 time, each 1-3 is little
Time, 4-10 times that quality is dregs of a decoction quality of distilled water the most used, merge extract, filter, filtrate reduced in volume is to medicinal material
0.5-2 times of quality, obtains decocting liquid；
6) by clarified dose of the decocting liquid process of step 5) gained, centrifugal, supernatant adds 95% ethanol of 3-5 mass times, stirs
Even, centrifugal, gained sediment is vacuum dried, and obtains sunflower disk polysaccharide；
7) by the sunflower disk general flavone of step 3) gained, the sunflower disk TA of step 4) gained and the certain herbaceous plants with big flowers of step 6) gained
Floral disc polysaccharide mixes, and obtains sunflower disk extract.
5. sunflower disk extract as claimed in claim 4, it is characterised in that described storng-acid cation exchange resin is 732
Type or HZ002 type, described fining agent is shitosan.
6. a pharmaceutical composition, it is characterised in that containing the sunflower disk extract as described in one of claim 1-5 with optional
The pharmaceutically acceptable auxiliary material in ground.
7. pharmaceutical composition as claimed in claim 6, it is characterised in that for tablet, capsule, pill, granule, powder,
Micropill, solution, syrup, emulsion or injection.
8. the preparation method of the sunflower disk extract as described in one of claim 1-5, it is characterised in that comprise the following steps:
1) being pulverized by sunflower disk, add the 50%-95% ethanol of 4-10 mass times, heating and refluxing extraction 1-3 time, each 1-3 is little
Time, merge extract, filter, 0.5-2 times of filtrate reduced in volume to quality of medicinal material, obtain alcohol extract；
2) alcohol extract of step 1) gained is filtered, take filtrate；
3) by step 2) filtrate of gained is after storng-acid cation exchange resin fully adsorbs, first with distilled water with 4-6BV/h
Flow velocity wash away water-solubility impurity, then elute with the flow velocity of 1-3BV/h with the 50%-95% ethanol of 5-10 mass times, collect
Eluent, reduced pressure concentration, it is dried, obtains sunflower disk general flavone；
4) by molten with the 50%-95% ethanol containing ammoniacal liquor 1%-5% for the storng-acid cation exchange resin that carries out in step 3) eluting
Liquid elutes with the flow velocity of 1-3BV/h, collects eluent, reduced pressure concentration, is dried, obtains sunflower disk TA；
5) being dried by the dregs of a decoction after step 1) alcohol extracting, after waving most ethanol, then by distilled water refluxing extraction 1-3 time, each 1-3 is little
Time, 4-10 times that quality is dregs of a decoction quality of distilled water the most used, merge extract, filter, filtrate reduced in volume is to medicinal material
0.5-2 times of quality, obtains decocting liquid；
6) by clarified dose of the decocting liquid process of step 5) gained, centrifugal, supernatant adds 95% ethanol of 3-5 mass times, stirs
Even, centrifugal, gained sediment is vacuum dried, and obtains sunflower disk polysaccharide；
7) by the sunflower disk general flavone of step 3) gained, the sunflower disk TA of step 4) gained and the certain herbaceous plants with big flowers of step 6) gained
Floral disc polysaccharide mixes, and obtains sunflower disk extract.
9. the purposes in preparing anti-gout drugs of the sunflower disk extract as described in one of claim 1-5.
The purposes in preparing anti-gout drugs of the pharmaceutical composition the most as claimed in claims 6 or 7.
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| CN107198708A (en) * | 2017-05-18 | 2017-09-26 | 上海中医药大学 | A kind of sunflower disk extract and its production and use |
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| CN109288884B (en) * | 2018-12-17 | 2021-09-07 | 广州诗美化妆品有限公司 | Preparation method and application of sunflower extract with soothing and repairing effects |
| CN110251545A (en) * | 2019-06-20 | 2019-09-20 | 中国药科大学 | A kind of sunflower disc extract and its preparation method and application |
| CN110478470A (en) * | 2019-08-28 | 2019-11-22 | 长春中医药大学 | A kind of anti-gout composition of the small-molecular peptides containing sunflower disk and preparation method thereof |
| CN116808172A (en) * | 2023-01-07 | 2023-09-29 | 王晓娟 | Sunflower disc liposome and application thereof in preparation of products for reducing uric acid and dissolving tophus |
| CN116808172B (en) * | 2023-01-07 | 2024-02-13 | 龚曙初 | Sunflower disc peptide, composite liposome and application thereof in preparation of products for reducing uric acid and dissolving tophus |
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