CN105861304B - A kind of PCR thermocirculators - Google Patents
A kind of PCR thermocirculators Download PDFInfo
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- CN105861304B CN105861304B CN201610253029.XA CN201610253029A CN105861304B CN 105861304 B CN105861304 B CN 105861304B CN 201610253029 A CN201610253029 A CN 201610253029A CN 105861304 B CN105861304 B CN 105861304B
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- 238000005538 encapsulation Methods 0.000 claims abstract description 54
- 238000001816 cooling Methods 0.000 abstract description 7
- 239000000463 material Substances 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 abstract 1
- 239000010408 film Substances 0.000 description 48
- 238000002834 transmittance Methods 0.000 description 23
- 238000009413 insulation Methods 0.000 description 19
- 239000012774 insulation material Substances 0.000 description 16
- UEXCJVNBTNXOEH-UHFFFAOYSA-N Ethynylbenzene Chemical group C#CC1=CC=CC=C1 UEXCJVNBTNXOEH-UHFFFAOYSA-N 0.000 description 14
- -1 Oxygen alkane Chemical class 0.000 description 14
- 239000000203 mixture Substances 0.000 description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical class O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 10
- 238000010438 heat treatment Methods 0.000 description 10
- 229920001169 thermoplastic Polymers 0.000 description 10
- 239000004416 thermosoftening plastic Substances 0.000 description 10
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 10
- 229920002554 vinyl polymer Polymers 0.000 description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 9
- BOTDANWDWHJENH-UHFFFAOYSA-N Tetraethyl orthosilicate Chemical compound CCO[Si](OCC)(OCC)OCC BOTDANWDWHJENH-UHFFFAOYSA-N 0.000 description 8
- 229920003086 cellulose ether Polymers 0.000 description 8
- 239000000919 ceramic Substances 0.000 description 8
- 229910021389 graphene Inorganic materials 0.000 description 8
- 229910002804 graphite Inorganic materials 0.000 description 8
- 239000010439 graphite Substances 0.000 description 8
- 239000005543 nano-size silicon particle Substances 0.000 description 8
- 235000012239 silicon dioxide Nutrition 0.000 description 8
- NKSJNEHGWDZZQF-UHFFFAOYSA-N ethenyl(trimethoxy)silane Chemical class CO[Si](OC)(OC)C=C NKSJNEHGWDZZQF-UHFFFAOYSA-N 0.000 description 6
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 5
- 229910052802 copper Inorganic materials 0.000 description 5
- 239000010949 copper Substances 0.000 description 5
- 150000002148 esters Chemical class 0.000 description 5
- 239000011810 insulating material Substances 0.000 description 5
- 238000010030 laminating Methods 0.000 description 5
- 239000002184 metal Substances 0.000 description 5
- 229910052751 metal Inorganic materials 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 239000005439 thermosphere Substances 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 3
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910000077 silane Inorganic materials 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 150000001345 alkine derivatives Chemical group 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Laminated Bodies (AREA)
- Testing Resistance To Weather, Investigating Materials By Mechanical Methods (AREA)
Abstract
The invention discloses a kind of PCR thermocirculators, belong to experiment consumptive material field.A kind of PCR thermocirculators of the present invention, including sample encapsulation disk, the sample encapsulation disk includes upper thermal insulating layer connected in sequence from top to bottom, sample layer and lower heat-conducting layer, and the sample layer includes siding and sample, and the sample is placed in siding.A kind of PCR thermocirculators of the present invention have processing easy to produce, can ensure that all samples evenly and rapidly heat up cooling, improve the rate of temperature fall that heats up, small, the characteristics of convenient for practicality.
Description
Technical field
The present invention relates to a kind of thermocirculator, particularly a kind of PCR thermocirculators.
Background technology
PCR（Polymerase Chain Reaction）It is a kind of molecular biotechnology, specific DNA pieces can be expanded in vitro
Section.The technology has been widely used for molecule diagnosis at present, precisely medical treatment, modern agriculture, the fields such as food security.Absolutely mostly
In several PCR reactions, it is required for carrying out sample periodically heating cooling to realize the amplification of specific DNA fragmentation.
The water-bath of prior art generally use or common metal bathe to realize thermal cycle.Water-bath thermal cycle by by sample repeatedly
It is immersed in and realizes the heating of sample and temperature-fall period in two temperature baths of high temperature and low temperature.But since water-bath cycle needs to match
Temperature bath is put, to be also equipped with corresponding inflow and outflow device, causes equipment heavy, is not convenient to use.Simultaneously as sample needs
It impregnates in water, therefore has high requirement to the tightness of sample encapsulation.And when water-bath is used to recycle, it has not been convenient to
Carry out the PCR operations of real-time quantitative.Common metal is bathed by the way that sample is placed in Reagent Tube, and Reagent Tube is inserted into thick sheet metal
In, then pass through heating and temperature-fall period of the realization of being heated and radiated to thick sheet metal to sample.But due to common metal
The contact surface of metallic plate and Reagent Tube is not plane in bath, thus easily generate sample uneven heating it is even the phenomenon that, to Reagent Tube,
The processing technology requirement of metallic plate etc. is high, and production and processing is of high cost.
Invention content
The goal of the invention of the present invention is：In view of the above problems, a kind of processing easy to produce is provided, can be ensured
All samples evenly and rapidly heat up cooling, improve heating rate of temperature fall, small, convenient for practical PCR thermocirculators.
The technical solution adopted by the present invention is as follows：
A kind of PCR thermocirculators of the present invention, including sample encapsulation disk, the sample encapsulation disk include from up to
Under upper thermal insulating layer connected in sequence, sample layer and lower heat-conducting layer, the sample layer includes siding and sample, and the sample is placed in wall
In plate.
Due to employing lower heat-conducting layer design in the above-mentioned technical solutions, the thickness of the bottom wall of sample encapsulation disk is substantially reduced
Degree, the contact wall for making sample encapsulation disk and heat-conduction medium is thinning, improves sample encapsulation disk bottom thermal transfer efficiency, while can
The uniformity coefficient that sample encapsulation tray bottom is heated is improved, so as to which sample be enable evenly and rapidly to heat up and cool down.
A kind of PCR thermocirculators of the present invention are uniformly provided with through-hole on the siding, and the sample is placed in through-hole.
By adopting the above-described technical solution, being provided with through-hole on siding, siding is connected with lower heat-conducting layer, and lower heat-conducting layer is with leading to
The side wall in hole forms a sample container, then heat-conducting layer is descended directly as sample container bottom wall, so as to further reduce sample envelope
The thickness of the bottom wall of sabot further improves sample encapsulation disk bottom thermal transfer efficiency, ensures sample evenly and rapidly to greatest extent
Heating and cooling.
Through-hole on siding can arrange by hole battle array, and hole battle array can have different designs, can be 96 holes or 384 holes etc..
A kind of PCR thermocirculators of the present invention, the siding upper and lower surface are equipped with concave station, and it is logical that the concave station is set on two
Between hole.
By adopting the above-described technical solution, upper thermal insulating layer is overlying on upper surface and the following table of siding with lower heat-conducting layer respectively
The side wall of face, upper thermal insulating layer, lower heat-conducting layer and through-hole forms a complete sample container, upper thermal insulating layer and lower heat-conducting layer difference
As the upper cover and bottom wall of sample container, and the concave station between siding two through hole can be overlying on siding in lower heat conduction and upper thermal insulating layer
When, container only is formed with through-hole, so as to be only in direct contact with sample, without being in direct contact with siding other faces, and disperses heat
Amount reduces thermal losses, so as to which heat concentration is sent at sample.
A kind of PCR thermocirculators of the present invention, the upper thermal insulating layer are made of high thermal insulation film, the lower heat-conducting layer
It is made of high-termal conductivity film, the siding is made of thermal insulation material.
By adopting the above-described technical solution, thermal losses can be reduced, so as to which heat concentration is sent at sample.
A kind of PCR thermocirculators of the present invention, the sample encapsulation disk top are equipped with light-transmitting plate, the sample encapsulation disk
Lower surface is connected with heat transfer unit (HTU) and radiator.
By adopting the above-described technical solution, light-transmitting plate is made of the material to visible transparent, light-transmitting plate is to selected
Exciting light and fluorescent transparent, which is arranged on sample encapsulation disk and puts, can be formed between thermocirculator component
Air layer can be passed through the air of heating to prevent in thermal cycle water condensation in the upper wall of container.
A kind of PCR thermocirculators of the present invention, the heat transfer unit (HTU) include metallic plate, and the metallic plate lower surface is connected with
Calorifics module；The radiator includes radiator and fan, and the radiator is connected with calorifics module, and the fan, which is set on, to be dissipated
Below hot device.
By adopting the above-described technical solution, metallic plate can be further ensured that and allow sample thermally equivalent, radiator and heat
Learn the function that module then realizes heating and cooling.
A kind of PCR thermocirculators of the present invention, the thickness of the sample disc are 0.1 ~ 0.4cm, the thickness of the light-transmitting plate
It spends for 0.5 ~ 2.5cm, the thickness of the heat transfer unit (HTU) is 0.1 ~ 2.0cm.
By adopting the above-described technical solution, the thickness of each layer is optimum range, it can be while performance be ensured
Obtain best heating rate of temperature fall.
A kind of PCR thermocirculators of the present invention, the light-transmitting plate is by 20 ~ 50 parts of half silicon of vinyl trimethoxy of mass parts
Oxygen alkane, 10 ~ 20 parts of vinyltrimethoxysilanes and 15 ~ 30 parts of tetraethyl orthosilicate compositions.
By adopting the above-described technical solution, light-transmitting plate has good light transmittance.Wherein, half silicon of vinyl trimethoxy
Oxygen alkane can be the arbitrary value in 20 ~ 50 parts, such as 23 parts, 25 parts, 27 parts, 38 parts, 42 parts, 46 parts etc.；Vinyl trimethoxy
Base silane can be the arbitrary value in 10 ~ 20 parts, such as 14 parts, 16 parts, 17 parts etc.；Tetraethyl orthosilicate can be 15 ~ 30 parts
In arbitrary value, such as 18 parts, 21 parts, 24 parts, 28 parts etc..
Preferably, when light-transmitting plate is by 47 parts of half siloxanes of vinyl trimethoxy of mass parts, 18 parts of vinyl trimethoxies
It is best when silane and 18 parts of tetraethyl orthosilicate compositions.
A kind of PCR thermocirculators of the present invention, the lower heat-conducting layer is by 40 ~ 70 parts of poly-methyl methacrylates of mass parts
Ester, 20 ~ 28 parts of nano silicon dioxides, 0 ~ 10 part of expanded porous graphite, 5 ~ 8 parts of graphenes, 7 ~ 18 parts of cellulose ethers and 10 ~ 16 parts
Coagulator forms.
By adopting the above-described technical solution, heat-conducting layer have good thermal conductivity, while have good flexibility and
Ductility, can reach the thickness of micron level still has good toughness, and installing under conditions of sufficiently thin can after sample
Guarantee will not bring to light.
Wherein polymethyl methacrylate can be the arbitrary value in 40 ~ 70 parts, such as 43 parts, 48 parts, 52 parts, 55 parts,
60 parts, 68 parts etc.；Nano silicon dioxide can be the arbitrary value in 20 ~ 28 parts, such as 25 parts, 27 parts etc.；Expanded porous graphite
Can be the arbitrary value in 0 ~ 10 part, such as 4 parts, 6 parts, 7 parts, 8 parts etc.；Graphene can be the arbitrary value in 5 ~ 8 parts, such as
6 parts, 7 parts etc.；Cellulose ether can be the arbitrary value in 7 ~ 18 parts, such as 10 parts, 13 parts, 15 parts, 17 parts etc.；Coagulator can be with
For the arbitrary value in 10 ~ 16 parts, such as 12 parts, 13 parts, 15 parts etc..
Preferably, heat-conducting layer is by 68 parts of polymethyl methacrylates of mass parts instantly, and 28 parts of nano silicon dioxides, 4 parts swollen
It is best when swollen porous graphite, 8 parts of graphenes, 14 parts of cellulose ethers and 15 parts of coagulator compositions.
A kind of PCR thermocirculators of the present invention, the upper thermal insulating layer are made of high thermal insulation film, and the high thermal insulation is thin
Film is the aeroge with high-permeability multi-branched nanoporous three-dimensional net structure, and the upper thermal insulating layer is by mass parts 10 ~ 30
Part eakleite-aeroge, 10 ~ 15 parts of ceramic fibre-aeroges and 5 ~ 10 parts poly- (2- methoxyl group -5- octyloxies) are to benzene second
Alkynes forms.
By adopting the above-described technical solution, upper thermal insulating layer heat-proof quality is good, it can effectively completely cut off ambient temperature to sample
Sample in encapsulation disk is improved the accuracy of heating, cooling, improves the precision to sample detection by external influence；Simultaneously
It is also preferable to the light transmittance of 450 ~ 580nm wavelength with good light transmittance.
Wherein, eakleite-aeroge can be the arbitrary value in 10 ~ 30 parts, such as 14 parts, 19 parts, 21 parts, 23 parts,
27 parts etc., ceramic fibre-aeroge can be the arbitrary value in 10 ~ 15 parts, such as 12 parts, 13 parts, 14 parts etc., poly- (2- methoxies
Base -5- octyloxies) can be the arbitrary value in 5 ~ 10 parts to phenylacetylene, such as 7 parts, 9 parts etc..
Preferably, upper thermal insulating layer by mass parts by 21 parts of eakleite-aeroges of mass parts, 12 parts of ceramic fibre-airsettings
When glue and 10 parts poly- (2- methoxyl group -5- octyloxies) form phenylacetylene, heat insulation is best, while has good light transmission
Rate, and there is certain gain effect to the wavelength of 450 ~ 580nm.
A kind of PCR thermocirculators of the present invention, the thickness of the upper thermal insulating layer is 25 ~ 500 μm, the lower heat-conducting layer
Thickness is 10 ~ 50 μm.
In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
1st, a kind of simple and reasonable, processing easy to produce is provided, can ensure that sample is uniform, quickly heat up cooling,
Convenient for practical PCR thermocirculators.
The 2nd, a kind of thickness for the bottom wall for greatly reducing sample encapsulation disk is provided, make connecing for sample encapsulation disk and heat-conduction medium
It is thinning to touch wall, improves sample encapsulation disk bottom thermal transfer efficiency, improves the uniformity coefficient that sample encapsulation tray bottom is heated.
Description of the drawings
Fig. 1 is sample encapsulation disk structure diagram；
Fig. 2 is sample encapsulation disk vertical view；
Fig. 3 is a kind of structure diagram of PCR thermocirculators；
Fig. 4 is a kind of partial enlarged view in PCR thermocirculators A portions.
It is marked in figure：1 is light-transmitting plate, and 2 be upper thermal insulating layer, and 3 be sample, and 4 be siding, and 5 be lower heat-conducting layer, and 6 be metallic plate,
7 be calorifics module, and 8 be radiator, and 9 be fan.
Specific embodiment
Below in conjunction with the accompanying drawings, the present invention is described in detail.
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to the accompanying drawings and embodiments, it is right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
Embodiment 1
As shown in Figures 1 to 4, a kind of PCR thermocirculators, including sample encapsulation disk, sample encapsulation disk include from up to
Under upper thermal insulating layer 2 connected in sequence, sample layer and lower heat-conducting layer 5, sample layer includes siding 4 and sample 3, and sample 3 is placed in siding 4
It is interior, through-hole is uniformly provided on siding 4, Kong Zhen arranges by 384 holes, and siding 4 is 384 orifice plates, and sample 3 is placed in through-hole, on siding 4
Lower surface is equipped with concave station, and concave station is set between two through hole, and upper thermal insulating layer 2 is overlying on the upper surface of siding 4 with lower heat-conducting layer 5 respectively
And lower surface, upper thermal insulating layer 2, the side wall of lower heat-conducting layer 5 and through-hole form a complete sample container, upper thermal insulating layer 2 and under
Heat-conducting layer 5 respectively as sample container upper cover and bottom wall, and the concave station between 4 two through hole of siding and upper thermal insulating layer 2 and under lead
Thermosphere 5 does not contact.The thickness of upper thermal insulating layer 2 is 500 μm, and the thickness of lower heat-conducting layer 5 is 50 μm.
Light-transmitting plate 1 is equipped with above sample encapsulation disk, sample encapsulation disk lower surface is connected with heat transfer unit (HTU) and radiator.Heat transfer
Device includes metallic plate 6, and 6 lower surface of metallic plate is connected with calorifics module 7；Radiator includes radiator 8 and fan 9, radiator 8
It is connected with calorifics module 7, fan 9 is set on 8 lower section of radiator.Metallic plate is made of copper.The overall thickness of sample disc is 0.4cm, thoroughly
The thickness of tabula rasa 1 is 2.5cm, and the thickness of heat transfer unit (HTU) is 2.0cm.
Upper thermal insulating layer 2 is made of high thermal insulation film, which is more with high-permeability multi-branched nanometer
The aeroge of hole three-dimensional net structure, by 10 parts of eakleite-aeroges of mass parts, 10 parts of ceramic fibre-aeroges and 5 parts it is poly-
(2- methoxyl group -5- octyloxies) forms phenylacetylene, and the thermal conductivity of the high thermal insulation film is 0.043W/（m·K）, to visible
Light light transmittance is 83.7%, and the light transmittance to 450 ~ 580nm wavelength is 86.3%.
Lower heat-conducting layer 5 is made of high-termal conductivity film, and the high-termal conductivity film is by 40 parts of poly-methyl methacrylates of mass parts
Ester, 20 parts of nano silicon dioxides, 0 part of expanded porous graphite, 5 parts of graphenes, 7 parts of cellulose ethers and 10 parts of coagulator compositions, should
The thermal conductivity of high hot film is 1.315W/（m·K）, the load-bearing of the hot film of the height is 0.8g/cm2
Siding 4 is made of thermal insulation material, the thermal insulation material by 20 parts of half siloxanes of vinyl trimethoxy of mass parts,
10 parts of vinyltrimethoxysilanes and 15 parts of tetraethyl orthosilicate compositions, the heat-insulating material are 92.6% to visible light transmittance rate,
Light transmittance to 450 ~ 580nm wavelength is 94.7%.
When in use, step 1：Siding 4 is made with thermal insulation material, through-hole has been opened up on siding 4；Step 2：It will
The siding made in step 1 and high-conductivity it is film adhered together, laminating type can be that chemistry is pasted, thermoplastic
Encapsulation, high voltage pack etc., using the film of high-termal conductivity as the bottom for holding sample, and the hole wall of siding then conduct in step 1
The chamber wall of sample container；Step 3：Prepared sample is injected in sample container；Step 4：To container obtained above
Obtained sample encapsulation disk is packaged, the upper surface of one high thermal insulation film and said vesse is fit together, fitting
Mode can be that chemistry is pasted, thermoplastic package, and high voltage pack etc. uses chemistry stickup or high voltage pack as most preferably.
Embodiment 2
As shown in Figures 1 to 4, a kind of PCR thermocirculators, including sample encapsulation disk, sample encapsulation disk include from up to
Under upper thermal insulating layer 2 connected in sequence, sample layer and lower heat-conducting layer 5, sample layer includes siding 4 and sample 3, and sample 3 is placed in siding 4
It is interior, through-hole is uniformly provided on siding 4, Kong Zhen arranges by 384 holes, and siding 4 is 384 orifice plates, and sample 3 is placed in through-hole, on siding 4
Lower surface is equipped with concave station, and concave station is set between two through hole, and upper thermal insulating layer 2 is overlying on the upper surface of siding 4 with lower heat-conducting layer 5 respectively
And lower surface, upper thermal insulating layer 2, the side wall of lower heat-conducting layer 5 and through-hole form a complete sample container, upper thermal insulating layer 2 and under
Heat-conducting layer 5 respectively as sample container upper cover and bottom wall, and the concave station between 4 two through hole of siding and upper thermal insulating layer 2 and under lead
Thermosphere 5 does not contact.The thickness of upper thermal insulating layer 2 is 25 μm, and the thickness of lower heat-conducting layer 5 is 10 μm.
Light-transmitting plate 1 is equipped with above sample encapsulation disk, sample encapsulation disk lower surface is connected with heat transfer unit (HTU) and radiator.Heat transfer
Device includes metallic plate 6, and 6 lower surface of metallic plate is connected with calorifics module 7；Radiator includes radiator 8 and fan 9, radiator 8
It is connected with calorifics module 7, fan 9 is set on 8 lower section of radiator.Metallic plate is made of copper.The overall thickness of sample disc is 0.1cm, thoroughly
The thickness of tabula rasa 1 is 0.5cm, and the thickness of heat transfer unit (HTU) is 0.1cm.
Upper thermal insulating layer 2 is made of high thermal insulation film, which is more with high-permeability multi-branched nanometer
The aeroge of hole three-dimensional net structure, by 30 parts of eakleite-aeroges of mass parts, 15 parts of ceramic fibre-aeroges and 10 parts
Poly- (2- methoxyl group -5- octyloxies) forms phenylacetylene, and the thermal conductivity of the high thermal insulation film is 0.047W/（m·K）, pair can
It is 82.0% to see light light transmittance, and the light transmittance to 450 ~ 580nm wavelength is 82.9%.
Lower heat-conducting layer 5 is made of high-termal conductivity film, and the high-termal conductivity film is by 70 parts of poly-methyl methacrylates of mass parts
Ester, 28 parts of nano silicon dioxides, 10 parts of expanded porous graphite, 8 parts of graphenes, 18 parts of cellulose ethers and 16 parts of coagulator groups
Into the thermal conductivity of the hot film of the height is 1.420W/（m·K）, the load-bearing of the hot film of the height is 1.1 g/cm2
Siding 4 is made of thermal insulation material, the thermal insulation material by 50 parts of half siloxanes of vinyl trimethoxy of mass parts,
20 parts of vinyltrimethoxysilanes and 30 parts of tetraethyl orthosilicate compositions, the heat-insulating material are 93.1% to visible light transmittance rate,
Light transmittance to 450 ~ 580nm wavelength is 93.7%.
When in use, step 1：Siding 4 is made with thermal insulation material, through-hole has been opened up on siding 4；Step 2：It will
The siding made in step 1 and high-conductivity it is film adhered together, laminating type can be that chemistry is pasted, thermoplastic
Encapsulation, high voltage pack etc., using the film of high-termal conductivity as the bottom for holding sample, and the hole wall of siding then conduct in step 1
The chamber wall of sample container；Step 3：Prepared sample is injected in sample container；Step 4：To container obtained above
Obtained sample encapsulation disk is packaged, the upper surface of one high thermal insulation film and said vesse is fit together, fitting
Mode can be that chemistry is pasted, thermoplastic package, and high voltage pack etc. uses chemistry stickup or high voltage pack as most preferably.
Embodiment 3
As shown in Figures 1 to 4, a kind of PCR thermocirculators, including sample encapsulation disk, sample encapsulation disk include from up to
Under upper thermal insulating layer 2 connected in sequence, sample layer and lower heat-conducting layer 5, sample layer includes siding 4 and sample 3, and sample 3 is placed in siding 4
It is interior, through-hole is uniformly provided on siding 4, Kong Zhen arranges by 384 holes, and siding 4 is 384 orifice plates, and sample 3 is placed in through-hole, on siding 4
Lower surface is equipped with concave station, and concave station is set between two through hole, and upper thermal insulating layer 2 is overlying on the upper surface of siding 4 with lower heat-conducting layer 5 respectively
And lower surface, upper thermal insulating layer 2, the side wall of lower heat-conducting layer 5 and through-hole form a complete sample container, upper thermal insulating layer 2 and under
Heat-conducting layer 5 respectively as sample container upper cover and bottom wall, and the concave station between 4 two through hole of siding and upper thermal insulating layer 2 and under lead
Thermosphere 5 does not contact.The thickness of upper thermal insulating layer 2 is 60 μm, and the thickness of lower heat-conducting layer 5 is 40 μm.
Light-transmitting plate 1 is equipped with above sample encapsulation disk, sample encapsulation disk lower surface is connected with heat transfer unit (HTU) and radiator.Heat transfer
Device includes metallic plate 6, and 6 lower surface of metallic plate is connected with calorifics module 7；Radiator includes radiator 8 and fan 9, radiator 8
It is connected with calorifics module 7, fan 9 is set on 8 lower section of radiator.Metallic plate is made of copper.The overall thickness of sample disc is 0.3cm, thoroughly
The thickness of tabula rasa 1 is 1.0cm, and the thickness of heat transfer unit (HTU) is 0.8cm.
Upper thermal insulating layer 2 is made of high thermal insulation film, which is more with high-permeability multi-branched nanometer
The aeroge of hole three-dimensional net structure, by 20 parts of eakleite-aeroges of mass parts, 12 parts of ceramic fibre-aeroges and 8 parts it is poly-
(2- methoxyl group -5- octyloxies) forms phenylacetylene, and the thermal conductivity of the high thermal insulation film is 0.052W/（m·K）, to visible
Light light transmittance is 81.5%, and the light transmittance to 450 ~ 580nm wavelength is 83.0%.
Lower heat-conducting layer 5 is made of high-termal conductivity film, and the high-termal conductivity film is by 57 parts of poly-methyl methacrylates of mass parts
Ester, 25 parts of nano silicon dioxides, 4 parts of expanded porous graphite, 7 parts of graphenes, 16 parts of cellulose ethers and 14 parts of coagulator compositions, should
The thermal conductivity of high hot film is 1.270W/（m·K）, the load-bearing of the hot film of the height is 0.9g/cm2
Siding 4 is made of thermal insulation material, the thermal insulation material by 36 parts of half siloxanes of vinyl trimethoxy of mass parts,
18 parts of vinyltrimethoxysilanes and 23 parts of tetraethyl orthosilicate compositions, the heat-insulating material are 94.1% to visible light transmittance rate,
Light transmittance to 450 ~ 580nm wavelength is 92.4%.
When in use, step 1：Siding 4 is made with thermal insulation material, through-hole has been opened up on siding 4；Step 2：It will
The siding made in step 1 and high-conductivity it is film adhered together, laminating type can be that chemistry is pasted, thermoplastic
Encapsulation, high voltage pack etc., using the film of high-termal conductivity as the bottom for holding sample, and the hole wall of siding then conduct in step 1
The chamber wall of sample container；Step 3：Prepared sample is injected in sample container；Step 4：To container obtained above
Obtained sample encapsulation disk is packaged, the upper surface of one high thermal insulation film and said vesse is fit together, fitting
Mode can be that chemistry is pasted, thermoplastic package, and high voltage pack etc. uses chemistry stickup or high voltage pack as most preferably.
Embodiment 4
As shown in Fig. 1, Fig. 3 and Fig. 4, a kind of PCR thermocirculators, including sample encapsulation disk, sample encapsulation disk include from
The upper thermal insulating layer 2 connected in sequence under, sample layer and lower heat-conducting layer 5, sample layer include siding 4 and sample 3, and sample 3 is placed in
In siding 4, through-hole is uniformly provided on siding 4, Kong Zhen arranges by 96 holes, and siding 4 is 96 orifice plates, and sample 3 is placed in through-hole, siding
4 upper and lower surfaces are equipped with concave station, and concave station is set between two through hole, and upper thermal insulating layer 2 is overlying on the upper of siding 4 respectively with lower heat-conducting layer 5
The side wall of surface and lower surface, upper thermal insulating layer 2, lower heat-conducting layer 5 and through-hole forms a complete sample container, upper thermal insulating layer 2
With lower heat-conducting layer 5 respectively as the upper cover and bottom wall of sample container, and the concave station between 4 two through hole of siding and upper thermal insulating layer 2 and
Lower heat-conducting layer 5 does not contact.The thickness of upper thermal insulating layer 2 is 127 μm, and the thickness of lower heat-conducting layer 5 is 38 μm.
Light-transmitting plate 1 is equipped with above sample encapsulation disk, sample encapsulation disk lower surface is connected with heat transfer unit (HTU) and radiator.Heat transfer
Device includes metallic plate 6, and 6 lower surface of metallic plate is connected with calorifics module 7；Radiator includes radiator 8 and fan 9, radiator 8
It is connected with calorifics module 7, fan 9 is set on 8 lower section of radiator.Metallic plate is made of copper.The overall thickness of sample disc is 0.32cm, thoroughly
The thickness of tabula rasa 1 is 0.9cm, and the thickness of heat transfer unit (HTU) is 1.3cm.
Upper thermal insulating layer 2 is made of high thermal insulation film, which is more with high-permeability multi-branched nanometer
The aeroge of hole three-dimensional net structure, by 21 parts of eakleite-aeroges of mass parts, 10 parts of ceramic fibre-aeroges and 6 parts it is poly-
(2- methoxyl group -5- octyloxies) forms phenylacetylene, and the thermal conductivity of the high thermal insulation film is 0.038W/（m·K）, to visible
Light light transmittance is 84.4%, and the light transmittance to 450 ~ 580nm wavelength is 86.9%.
Lower heat-conducting layer 5 is made of high-termal conductivity film, and the high-termal conductivity film is by 62 parts of poly-methyl methacrylates of mass parts
Ester, 23 parts of nano silicon dioxides, 3 parts of expanded porous graphite, 8 parts of graphenes, 13 parts of cellulose ethers and 10 parts of coagulator compositions, should
The thermal conductivity of high hot film is 1.535W/（m·K）, the load-bearing of the hot film of the height is 0.6g/cm2
Siding 4 is made of thermal insulation material, the thermal insulation material by 37 parts of half siloxanes of vinyl trimethoxy of mass parts,
12 parts of vinyltrimethoxysilanes and 27 parts of tetraethyl orthosilicate compositions, the heat-insulating material are 93.8% to visible light transmittance rate,
Light transmittance to 450 ~ 580nm wavelength is 94.1%.
When in use, step 1：Siding 4 is made with thermal insulation material, through-hole has been opened up on siding 4；Step 2：It will
The siding made in step 1 and high-conductivity it is film adhered together, laminating type can be that chemistry is pasted, thermoplastic
Encapsulation, high voltage pack etc., using the film of high-termal conductivity as the bottom for holding sample, and the hole wall of siding then conduct in step 1
The chamber wall of sample container；Step 3：Prepared sample is injected in sample container；Step 4：To container obtained above
Obtained sample encapsulation disk is packaged, the upper surface of one high thermal insulation film and said vesse is fit together, fitting
Mode can be that chemistry is pasted, thermoplastic package, and high voltage pack etc. uses chemistry stickup or high voltage pack as most preferably.
Embodiment 5
As shown in Figures 1 to 4, a kind of PCR thermocirculators, including sample encapsulation disk, sample encapsulation disk include from up to
Under upper thermal insulating layer 2 connected in sequence, sample layer and lower heat-conducting layer 5, sample layer includes siding 4 and sample 3, and sample 3 is placed in siding 4
It is interior, through-hole is uniformly provided on siding 4, Kong Zhen arranges by 384 holes, and siding 4 is 384 orifice plates, and sample 3 is placed in through-hole, on siding 4
Lower surface is equipped with concave station, and concave station is set between two through hole, and upper thermal insulating layer 2 is overlying on the upper surface of siding 4 with lower heat-conducting layer 5 respectively
And lower surface, upper thermal insulating layer 2, the side wall of lower heat-conducting layer 5 and through-hole form a complete sample container, upper thermal insulating layer 2 and under
Heat-conducting layer 5 respectively as sample container upper cover and bottom wall, and the concave station between 4 two through hole of siding and upper thermal insulating layer 2 and under lead
Thermosphere 5 does not contact.The thickness of upper thermal insulating layer 2 is 375 μm, and the thickness of lower heat-conducting layer 5 is 32 μm.
Light-transmitting plate 1 is equipped with above sample encapsulation disk, sample encapsulation disk lower surface is connected with heat transfer unit (HTU) and radiator.Heat transfer
Device includes metallic plate 6, and 6 lower surface of metallic plate is connected with calorifics module 7；Radiator includes radiator 8 and fan 9, radiator 8
It is connected with calorifics module 7, fan 9 is set on 8 lower section of radiator.Metallic plate is made of copper.The overall thickness of sample disc is 0.2cm, thoroughly
The thickness of tabula rasa 1 is 1.8cm, and the thickness of heat transfer unit (HTU) is 1.5cm.
Upper thermal insulating layer 2 is made of high thermal insulation film, which is more with high-permeability multi-branched nanometer
The aeroge of hole three-dimensional net structure, by 21 parts of eakleite-aeroges of mass parts, 12 parts of ceramic fibre-aeroges and 10 parts
Poly- (2- methoxyl group -5- octyloxies) forms phenylacetylene, and the thermal conductivity of the high thermal insulation film is 0.033W/（m·K）, pair can
It is 83.4% to see light light transmittance, and the light transmittance to 450 ~ 580nm wavelength is 85.2%.
Lower heat-conducting layer 5 is made of high-termal conductivity film, and the high-termal conductivity film is by 68 parts of polymethyl methacrylates, 28 parts
Nano silicon dioxide, 4 parts of expanded porous graphite, 8 parts of graphenes, 14 parts of cellulose ethers and 15 parts of coagulator compositions, the height are hot
The thermal conductivity of film is 1.577W/（m·K）, the load-bearing of the hot film of the height is 0.8g/cm2。
Siding 4 is made of thermal insulation material, the thermal insulation material by 47 parts of half siloxanes of vinyl trimethoxy of mass parts,
18 parts of vinyltrimethoxysilanes and 18 parts of tetraethyl orthosilicate compositions, the heat-insulating material are 94.1% to visible light transmittance rate,
Light transmittance to 450 ~ 580nm wavelength is 94.9%.
When in use, step 1：Siding 4 is made with thermal insulation material, through-hole has been opened up on siding 4；Step 2：It will
The siding made in step 1 and high-conductivity it is film adhered together, laminating type can be that chemistry is pasted, thermoplastic
Encapsulation, high voltage pack etc., using the film of high-termal conductivity as the bottom for holding sample, and the hole wall of siding then conduct in step 1
The chamber wall of sample container；Step 3：Prepared sample is injected in sample container；Step 4：To container obtained above
Obtained sample encapsulation disk is packaged, the upper surface of one high thermal insulation film and said vesse is fit together, fitting
Mode can be that chemistry is pasted, thermoplastic package, and high voltage pack etc. uses chemistry stickup or high voltage pack as most preferably.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should all be included in the protection scope of the present invention.
Claims (5)
1. a kind of PCR thermocirculators, it is characterised in that：Including sample encapsulation disk, the sample encapsulation disk is included from top to bottom
Upper thermal insulating layer connected in sequence（2）, sample layer and lower heat-conducting layer（5）, the sample layer include siding（4）；The siding（4）On
It is uniformly provided with through-hole, sample（3）It is placed in through-hole；Light-transmitting plate is equipped with above the sample encapsulation disk（1）, the sample encapsulation disk
Lower surface is connected with heat transfer unit (HTU) and radiator.
2. a kind of PCR thermocirculators as described in claim 1, it is characterised in that：The siding（4）Upper and lower surface is equipped with
Concave station, the concave station are set between two through hole.
3. a kind of PCR thermocirculators as described in claim 1, it is characterised in that：The heat transfer unit (HTU) includes metallic plate
（6）, the metallic plate（6）Lower surface is connected with calorifics module（7）；The radiator includes radiator（8）And fan（9）, institute
State radiator（8）With calorifics module（7）It is connected, the fan（9）Set on radiator（8）Lower section.
4. a kind of PCR thermocirculators as described in claim 1, it is characterised in that：The thickness of the sample disc for 0.1 ~
0.4cm, the light-transmitting plate（1）Thickness for 0.5 ~ 2.5cm, the thickness of the heat transfer unit (HTU) is 0.1 ~ 2.0cm.
5. a kind of PCR thermocirculators as described in claim 1, it is characterised in that：The upper thermal insulating layer（2）Thickness be 25
~ 500 μm, the lower heat-conducting layer（5）Thickness be 10 ~ 50 μm.
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| CN113801785A (en) * | 2021-09-30 | 2021-12-17 | 深圳市瑞沃德生命科技有限公司 | PCR thermal cycling device |
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| CN101735949B (en) * | 2008-11-05 | 2012-10-10 | 广达电脑股份有限公司 | variable temperature device |
| KR102032523B1 (en) * | 2010-01-12 | 2019-10-15 | 아람 바이오시스템 주식회사 | Two-Stage Thermal Convection Apparatus And Uses Thereof |
| CN103695309A (en) * | 2013-12-19 | 2014-04-02 | 江苏金太生命科技有限公司 | Novel PCR (Polymerase Chain Reaction) instrument |
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