CN105807038A - Blood cell analyzer, body fluid analysis method and control system thereof - Google Patents
Blood cell analyzer, body fluid analysis method and control system thereof Download PDFInfo
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- CN105807038A CN105807038A CN201610209662.9A CN201610209662A CN105807038A CN 105807038 A CN105807038 A CN 105807038A CN 201610209662 A CN201610209662 A CN 201610209662A CN 105807038 A CN105807038 A CN 105807038A
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
- G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/012—Red blood cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/012—Red blood cells
- G01N2015/014—Reticulocytes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/016—White blood cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1486—Counting the particles
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- Life Sciences & Earth Sciences (AREA)
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- Analytical Chemistry (AREA)
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Abstract
The invention provides a blood cell analyzer comprising a fluid device equipped with a sample aspiration nozzle for aspirating samples and prepare a test sample by mixing the sample aspirated by the aspiration nozzle and a reagent; a detection portion capable of detecting scattered light and fluorescent light acquired from the blood cells in the test sample flowing through a sheath flow cell; a controller for processing a testing result output from the detection portion to obtain an analysis result; and a display, wherein the controller enables the display to display an input frame for accepting the selection of a blood test mode and a body fluid test mode, if the blood test mode is selected, the placing mode of the sample at least can be assigned the following modes including a first mode of manually aspirating the sample by an operator and a second mode of automatically delivering and aspirating the sample; if the body fluid test mode is selected, just the first mode can be assigned to be used as the placing mode of the sample.
Description
The application is application number is 200810005239.2, the applying date be January 31 in 2008 day, be called the divisional application of the Chinese invention patent application submitted to by same applicant of " cellanalyzer, method for analyzing body fluid and control system thereof ".
Technical field:
The present invention relates to and a kind of can not only measure blood as sample, also can measure the cellanalyzer of other body fluid, method for analyzing body fluid and control system thereof beyond the blood such as cerebrospinal fluid (marrow liquid), hydrothorax (Pleural fluid) and ascites.
Background technology:
One of in clinical examination field, conventional cellanalyzer is analyzed with the blood picking up from health for tested sample, and the reference that this analysis result is monitored as diagnosis and treatment.
Meanwhile, the serious hope of clinical examination field can measure the body fluid beyond the blood such as cerebrospinal fluid easily.Usual body fluid is practically free of cell, but when having tumor and damage when ill or relevant organ, it finds that hemorrhage (hemocyte) and the cell such as abnormal cell, antibacterial.
No. 2003/0215890 publication of U.S. Patent Application Publication No. describes the technology about the cell measured with cellanalyzer in body fluid.Accordingly No. 2003/0215890 publication of U.S. Patent Application Publication No. describes, reagent components containing aldehyde, surfactant and cyclodextrin is mixed with cerebrospinal fluid (CSF), formation determination sample, made mensuration sample is analyzed, the cell in cytological map classification also counting brain spinal fluid according to Figure 11 of this publication A ~ Figure 11 G with ADVIA120 cytoanalyze.
But, according to technology disclosed on No. 2003/0215890 publication of U.S. Patent Application Publication No., only analyze cerebrospinal fluid (CSF) effectively as body fluid, be not analyzed about the such as body fluid such as ascites and hydrothorax.Scarcely containing the particle composition beyond hemocyte in usual cerebrospinal fluid, and in the body fluid beyond the cerebrospinal fluid such as ascites and hydrothorax, contain middle chrotoplast, macrophage and tumor cell etc. sometimes because of patient disease.So, when technical Analysis disclosed in using on No. 2003/0215890 publication of U.S. Patent Application Publication No. contains the body fluid of the particle composition beyond hemocyte, it is possible to cause and such as at certain cell compartment of cytological map, the particle composition beyond hemocyte occurs, it is impossible to the problem drawing Correct Analysis result.
Summary of the invention:
The scope of the present invention is only by appended claims defined, and in any degree, statement by this joint summary of the invention is not limit.
The cellanalyzer measuring hemocyte that the present invention the first side provides includes: mode determination setup unit, is used for setting humoral determination pattern；Instruction to start the measurement unit, for accepting the instruction starting to measure；Optical information acquiring unit, illumination measures sample, obtains optical information from cell contained by this mensuration sample；Analytic unit, after above-mentioned humoral determination pattern sets, when said determination start indicating member receive start measure instruction time, according to the above-mentioned optical information obtained in the said determination sample prepared by humoral specimen and leukocyte mensuration reagent, cell contained by this mensuration sample is at least categorized as the nucleated cell beyond leukocyte and leukocyte, to the Other nucleated cells differential count beyond leukocyte and leukocyte.
Leukocyte is divided into multinuclear leucocyte and monocyte by described analytic unit, respectively multinuclear leucocyte and monocyte is counted.
Described analytic unit calculates multinuclear leucocyte shared ratio or monocyte shared ratio in leukocyte in leukocyte.
Described analytic unit obtains full number of nucleated cells from the number of nucleated cells beyond leukocyte count and leukocyte, then obtains the ratio of the nucleated cell beyond leukocyte and full nucleated cell.
Cell contained by described mensuration sample is separated erythrocyte ghost by described analytic unit further.
When not setting described humoral determination pattern, after instruction to start the measurement unit accepts the instruction starting to measure, leukocyte differential count contained by described mensuration sample is several subclass by the described optical information that analytic unit obtains in sample according to measuring of preparing from blood preparation and leukocyte mensuration reagent, and counts.
It is also equipped with output unit, the display picture of the analysis result of analytic unit described in output display.
Scattering optical information that described optical information sends selected from cell, the fluorescence information that cell sends, cell extinction light absorb the combination of information and these information.
Described humoral specimen is selected from the liquid including cerebrospinal fluid, hydrothorax, ascites, pericardium liquid, joint fluid, the dialysis solution of peritoneal dialysis and intraperitoneal cleanout fluid.
Described nucleated cell selects the colony that free macrophage, middle chrotoplast, tumor cell, erythrocyte ghost and combination thereof are constituted.
The cellanalyzer that the present invention the second side provides includes: mode determination setup unit, is used for setting humoral determination pattern；Instruction to start the measurement unit, for accepting the instruction starting to measure；Aspirating specimen unit, is used for aspirating specimen；Measure sample and prepare unit, the specimen aspirated with aspirating specimen unit and leukocyte mensuration reagent formation determination sample；Optical information acquiring unit, illumination measures sample, obtains optical information from cell contained by this mensuration sample；Analytic unit, according to the above-mentioned optical information obtained, classifies to cell contained by said determination sample, and the above-mentioned cell counting to classification.After above-mentioned humoral determination pattern is set by said determination mode setting unit, when said determination start indicating member receive start measure instruction time, humoral specimen that unit just aspirates prepared by above-mentioned aspirating specimen unit by said determination sample and said determination sample prepared by above-mentioned leukocyte mensuration reagent, above-mentioned analytic unit is according to the above-mentioned optical information obtained from said determination sample, it is the cell beyond leukocyte and leukocyte by measuring cell divide contained in sample, and to the Other nucleated cells differential count beyond leukocyte and leukocyte.
When not setting described humoral determination pattern, after instruction to start the measurement unit accepts the instruction starting to measure, blood preparation and the described leukocyte mensuration reagent formation determination sample that the described aspirating specimen unit of unit aspirates prepared by described mensuration sample, leukocyte differential count contained by described mensuration sample is several subclass according to the described optical information obtained from described mensuration sample by described analytic unit, and counts.
The method for analyzing body fluid that the present invention the 3rd side provides includes: (a) step: set mode determination, in order to set humoral determination pattern；B () step: after humoral determination pattern sets, accepts the instruction starting to measure；C () step: after accepting the above-mentioned instruction starting and measuring, use up and irradiate the mensuration sample prepared by humoral specimen and leukocyte mensuration reagent, obtains optical information from cell contained by this mensuration sample；D () step: according to the above-mentioned optical information obtained, is at least categorized as nucleated cell beyond leukocyte and leukocyte by cell contained by said determination sample, and to Other nucleated cells differential count beyond leukocyte and leukocyte.
Described method for analyzing body fluid also includes step e: after accepting the described instruction starting and measuring, aspirates described humoral specimen, and is prepared described mensuration sample by the described humoral specimen aspirated and described leukocyte mensuration reagent.
It is multinuclear leucocyte and monocyte the step that multinuclear leucocyte and monocyte are counted respectively that described (d) step includes leukocyte differential count.
Described (d) step includes asking the multinuclear leucocyte ratio in leukocyte or the step of monocyte ratio in leukocyte.
The present invention the 4th side provides the control system of a kind of body fluid analysis instrument, including: a kind of mode determination setting control system, set mode determination, in order to set humoral determination pattern；The instruction of a kind of mensuration accepts control system, after humoral determination pattern sets, accepts the instruction starting to measure；A kind of optical information obtains control system, after accepting the above-mentioned instruction starting and measuring, uses up and irradiates the mensuration sample prepared by humoral specimen and leukocyte mensuration reagent, obtains optical information from cell contained by this mensuration sample；And a kind of cell divide and counting control system, according to the above-mentioned optical information obtained, cell contained by said determination sample is at least categorized as the nucleated cell beyond leukocyte and leukocyte, and to the Other nucleated cells differential count beyond leukocyte and leukocyte.
Leukocyte differential count can be multinuclear leucocyte and monocyte and multinuclear leucocyte and monocyte are counted respectively by described cell divide and counting control system.
Described cell divide and counting control system can calculate the multinuclear leucocyte ratio in leukocyte or monocyte ratio in leukocyte.
Described cell divide and counting control system can separate erythrocyte ghost further from cell contained by described mensuration sample.
Accompanying drawing illustrates:
Fig. 1 is the outside drawing of the cellanalyzer of an embodiment of the present invention.
Fig. 2 is the block diagram of analysis-e/or determining device.
Fig. 3 is the block diagram of fluid device.
Fig. 4 is the optical system display figure of white blood cell detection device.
Fig. 5 is the display figure of RBC/PLT detector.
Fig. 6 is the display figure of HGB detector.
Fig. 7 is the flow chart that specimen mensuration processes.
Fig. 8 is the accompanying drawing of the display picture for setting mode determination.
Fig. 9 is the flow chart that presequence processes.
Figure 10 is the ideograph measuring the scatterplot that the DIFF prepared by body fluid measures sample.
Figure 11 is the cellanalyzer measurement result comparison diagram with antithetic measurement result of embodiment.
Figure 12 is the ideograph measuring the scatterplot that the DIFF prepared by blood measures sample.
Figure 13 is the display picture of the measurement result of blood measuring pattern.
Figure 14 is the display picture of the measurement result of humoral determination pattern.
Figure 15 is the display picture of the measurement result of humoral determination pattern.
Figure 16 is the display picture of the measurement result of humoral determination pattern.
Figure 17 is the confirmation screen starting blank detection shown under humoral determination pattern.
Detailed description of the invention:
Below according to accompanying drawing, the specific embodiment of the present invention is described.
Fig. 1 show cellanalyzer 1.This cellanalyzer 1 is as the multinomial Automatic Blood Cell Analyzer for blood test, the blood preparation that specimen container (blood taking tube) is inner can be measured, obtain and represent the characteristic information of contained hemocyte feature in specimen, and be analyzed this characteristic information processing.This cellanalyzer 1 can also analysing body fluid.At the cellanalyzer of present embodiment, analyze object body fluid and refer to be present in endoceliac coelomic fluid beyond blood.Specifically refer to cerebrospinal fluid (marrow liquid, CSF: the hydrops of the ventricles of the brain and subarachnoid space), hydrothorax (Pleural fluid, PE: hydrothorax), ascites (seroperitoneum), pericardium liquid (chambers of the heart hydrops), joint fluid (synovial fluid: the liquid in joint, synovial capsule and stndon sheath) etc..The dialysis solution of peritoneal dialysis (CAPD) and intraperitoneal cleanout fluid etc. also can be analyzed as the one of body fluid.Generally almost without cell in these liquid, but when ill or relevant organ has tumor and damaged, it is possible to containing cells such as hemocyte, abnormal cell and antibacterials.Such as cerebrospinal fluid, can make following clinical deduction from analyzing result.Such as, if erythrocyte increases, then it is probably subarachnoid hemorrhage, if neutrophil cell increases, then can suspected of meningitis, if oxyphil cell increases, then can suspect and suffer from infectious disease (parasite and fungus), if mononuclear cell increases, then can suspect for Tuberculous meningitis and viral meningitis, if other cells increase, then can suspect and shift to meninges for tumor.As for ascites and hydrothorax etc., if possibly together with nucleated cell such as middle chrotoplast, macrophage and tumor cells except hemocyte, it is possible to as the index suspecting the diseases such as cancer, by analyzing the nucleated cell beyond this hemocyte, these indexs can be obtained.
Cellanalyzer 1 is processed, obtains the data processing equipment 3 of analysis result and constitutes by the determinator 2 that can measure the blood as sample and body fluid and the measurement result that determinator 2 is exported.Data processing equipment 3 includes controller 301, display 302 and input equipment 303.In FIG, determinator 2 and data processing equipment 3 respectively exist as a device, it is possible to unite two into one as a device.
Fig. 2 is the block diagram of the determinator 2 of cellanalyzer 1.As in figure 2 it is shown, determinator 2 includes hemocyte detection part 4, the analogue signal processor 5 that the output signal (analogue signal) detecting part 4 is processed, microcomputer 6, display operation part 7 and measures the device for mechanical part 8 of blood and body fluid.Device for mechanical part 8 comprises following fluid device 81.
Fig. 3 is the structured flowchart of fluid device 81.As it is shown on figure 3, fluid device 81 comprises aspirating specimen mouth 18, several reagent container 11, sampling valve 12 and reaction warehouse 13 ~ 17.Aspirating specimen mouth 18 aspirates specimen from specimen container, and this specimen is sent to sampling valve 12.The specimen of importing is divided into a certain amount of some deciles by sampling valve 12.This segmentation number is different because of mode determination (each mode determination), and the CBC type specimen measuring RBC number, leukocyte count, platelet count and hemoglobin concentration is divided into trisection.The CBC+DIFF pattern of leukocyte five classification, then specimen quadrisection is added again on above-mentioned CBC mode determination.CBC+DIFF mensuration project adds CBC+DIFF+RET pattern again that measure reticulocyte, is divided into five deciles.Equally, the CBC+DIFF+NRBC pattern mensuration project of CBC+DIFF pattern adding again erythroblast mensuration project is also that specimen is divided into five deciles.CBC+DIFF pattern+RET mensuration project adds CBC+DIFF+RET+NRBC pattern again that measure erythroblast, is then divided into six deciles.Above mode determination is the blood measuring pattern measuring blood entirely.Finally, in the humoral determination pattern measuring body fluid, specimen is divided into bisection.
Reagent (diluent) imports this sampling valve 12 from reagent container 11, and the specimen decile split is transported to reaction warehouse 13 ~ 17 and aftermentioned HGB detector 43 together with reagent.Reaction warehouse 13 is provided the sampling valve 12 a certain amount of specimen (decile), a certain amount of diluent and a certain amount of dyeing liquor that extract by not shown dosing pump, and these specimen and reagent are blended, prepares leukocyte four and classifies the mensuration sample of (DIFF).
As diluent, can suitably use the reagent " leukocyte hemolysin STROMATOLYSER-4DL " that SYSMEX Co., Ltd provides.This reagent contains surfactant, it is possible to lysed erythrocyte.Dyeing liquor can suitably use reagent that SYSMEX Co., Ltd provides " leukocyte four classify liquid STROMATOLYSER-4DS " equally.This dyeing liquor contains ethylene glycol, low alcohol, polymethine, and after above-mentioned diluent haemolysis, hemocyte composition is colored, and finally produces 50 times of dilution samples.
If selecting body fluid mode determination, then the condition to measure the same specimen amount of sample, same reagent and same reagent amount with this leukocyte four classification prepares leukocyte differential count mensuration sample.But, as described later, the leukocyte differential count leukocyte of humoral determination pattern is not four classes, but two classes.
These specimen and reagent are mixed by a certain amount of specimen, a certain amount of dilution hemolytic agent and a certain amount of dyeing liquor that reaction warehouse 14 is gathered by not shown dosing pump offer sampling valve 12, make erythroblast (NRBC) and measure with mensuration sample.
These specimen and reagent are mixed by a certain amount of specimen, a certain amount of dilution hemolytic agent and a certain amount of dyeing liquor that reaction warehouse 15 is gathered by not shown dosing pump offer sampling valve 12, make reticulocyte (RET) and measure with mensuration sample.
Reaction warehouse 16 is provided a certain amount of specimen that gathers of sampling valve 12 and a certain amount of dilution hemolytic agent by not shown dosing pump, these specimen and reagent is mixed, and makes leukocyte and basophil (WBC/BASO) measures with measuring sample.
These specimen and reagent are mixed by a certain amount of specimen that reaction warehouse 17 is gathered by not shown dosing pump offer sampling valve 12, a certain amount of diluent, make erythrocyte/platelet (RET/PLT) and measure with mensuration sample.
It addition, a certain amount of specimen of sampling valve 12 collection, a certain amount of dilution hemolytic agent also supply aftermentioned HGB detector 43.
Detection part 4 has the white blood cell detection device 41 of detection leukocyte.This white blood cell detection device 41 is also used for the detection of erythroblast and reticulocyte.Detection part 4, except white blood cell detection device 41, also has the RBC/PLT detector 42 measuring RBC number and platelet count and measures the HGB detector 43 of hematochrome amount in blood.
Above-mentioned white blood cell detection device 41 is mainly made up of fluorescence detector, specifically, is made up of the detector using flow cytometry.At this, namely so-called cell art measures physical property and the chemical property of cell and other biological particle, and so-called flow cytometry refers to: allow the method that these particles pass through from thread, are measured.Fig. 4 show the optical system of white blood cell detection device 41.In the figure, it is irradiated to, by collimating mirror 402, the hemocyte flowed through in sheath flow pool 403 from the light beam of laser luminescence diode 401 injection.This white blood cell detection device 41 detects forward scattering light intensity, lateral scattering light intensity and the lateral fluorescence intensity that the hemocyte in sheath flow pool 403 sends under laser irradiates, in this, as hemocyte characteristic parameter.
At this, scattering of light is that therefore light change phenomenon produced by its direct of travel owing to this particle of hemocyte becomes barrier on the direct of travel of light.Detect this scattering light and can obtain the particle characteristics information about particle size and composition.The scattering light that the direct of travel with institute irradiation light that forward scattering light refers to that particle sends is essentially identical.The characteristic information about particle (hemocyte) size can be obtained from forward scattering light.Lateral scattering just particle send with institute irradiation light scattering light slightly in vertical direction.The characteristic information about inside particles can be obtained from side scattered light.When laser is irradiated on hemocyte particle, lateral scattering light intensity depends on the complexity (quantity of the shape of core, size, density and granule) of cell interior.Therefore, utilize this characteristic of lateral scattering light intensity can classify (discriminating) hemocyte, and measure hemocyte quantity.Additionally, already described present embodiment takes the structure using forward scattering light and side scattered light as scattering light, but being not limited to this, as long as be obtained in that the scattered light signal analyzing required reflection particle characteristics, the angle of the optical axis of the light that scattering light irradiates through sheath flow pool for light source is not limit.
That fluorescent material of hemocyte that illumination is such as dyed, then send the light more longer than shone optical wavelength.Fluorescence intensity is to dye more good more strong, measures this fluorescence intensity and is achieved with the characteristic information about blood cell staining degree.Therefore, the difference according to (laterally) fluorescence intensity, it is possible to leukocyte is carried out classification etc. and measures.
As shown in Figure 4, flow through the forward scattering light that the hemocyte (leukocyte and erythroblast) of sheath flow pool 403 sends to be accepted by light emitting diode (forward scattering light optical collector) 406 by condenser lens 404 and pin hole 405.Side scattered light is accepted by photomultiplier tube (side scattered light optical collector) 411 by condenser lens 407, dichroic mirror 408, blooming 409 and pin hole 410.Lateral fluorescence is accepted by photomultiplier tube (lateral fluorescence optical collector) 412 by condenser lens 407 and dichroic mirror 408.From the suffered optical signal of each optical collector 406,411 and 412 output respectively through the analogue signal processor 5 being made up of amplifier 51,52,53 etc. be amplified with the analog signal processing such as waveform processing after, be transported to microcomputer 6.
Below, the structure with regard to RBC/PLT detector 42 illustrates.Fig. 5 is the brief configuration ideograph of RBC/PLT detector 42.RBC/PLT detector 42 can use sheath stream DC detection method to measure RBC number and platelet count.RBC/PLT detector 42 has sheath flow pool 42a as shown in Figure 5.This sheath flow pool 42a is provided with the adding mouth 42b of upward opening, and sample can add this adding mouth 42b from reaction warehouse 17.Sheath flow pool 42a also has upwards tapered taper sample storehouse 42c, and above-mentioned adding mouth 42b is just arranged in the center of inside of this sample storehouse 42c.42c upper end, sample storehouse is provided with hole 42d, and this hole 42d is just relative with adding mouth 42b center.The sample that measures provided for sample device upwards transports from the front end of adding mouth 42b, and meanwhile, front sheath fluid is fed to sample storehouse 42c, and front sheath fluid flows up and flows to hole 42d.At this, measuring sample and flow under the encirclement of front sheath fluid, taper sample storehouse 42c makes mensuration sample stream attenuate, and measures the hemocyte in sample one by one by hole 42d.Hole 42d sets electrode, is provided with DC current between this electrode.This signal of telecommunication, when measuring the change that sample flows through the D.C. resistance of hole 42d hole when 42d, is exported controller 25 by detection.Above-mentioned D.C. resistance can increase when hemocyte is by hole 42d, and therefore, this signal of telecommunication reflection hemocyte information by hole 42d, by carrying out signal processing to this signal of telecommunication, to erythrocyte and platelet count.
Hole 42d is arranged over the upwards lower recovery tube 42e extended.This recovery tube 42e is configured at by hole 42d and the sample storehouse 42c sample storehouse 42f being connected.Recovery tube 42e lower end separates with sample storehouse 42f inwall.Sample storehouse 42f has rear sheath fluid to provide, and hereafter sheath fluid flows downward along the exterior lateral area of the recovery tube 42e of sample storehouse 42f.The rear sheath fluid flow through outside recovery tube 42e between the inwall of recovery tube 42e lower end and sample storehouse 42f, flows to inside recovery tube 42e after arriving 42f lower end, sample storehouse.Therefore it is possible to prevent to be refluxed by the hemocyte of hole 42d, thus preventing the flase drop of hemocyte.
Structure with regard to HGB detector 43 illustrates below.HGB detector 43 can pass through SLS hemoglobin method and measure hematochrome amount (HGB).Fig. 6 is the oblique view of HGB detector 43 structure.HGB detector 43 has the sample pond 43a of dress dilution sample, passes through the light collecting element 43c of the transmission light of sample pond 43a to light emitting diode 43b luminous for sample pond 43a and reception.The quantitative blood of sampling valve 12 is diluted liquid and the dilution of certain hemolytic agent, preparation dilution sample by certain dilution rate.This hemolytic agent has the character that the hemoglobin in blood is converted to SLS-hemoglobin.This dilution sample feeds to sample pond 43a, deposits in sample pond 43a.In this case, making light emitting diode 43b luminous, transmission light is received by the light collecting element 43c every sample pond 43a and light emitting diode 43b relative configuration.The sent out wavelength light of light emitting diode 43b is prone to by SLS-hemoglobin absorption, and sample pond 43a is made up of the plastic material that light transmission is high, and therefore, what light collecting element 43c received is that light emitting diode 43b launches the transmission light after light is only diluted sample absorption.The signal of telecommunication corresponding with light harvesting amount (absorbance) is transported to microcomputer 6 by light collecting element 43c, and microcomputer 6 by the dulling luminosity ratio of this absorbance and the only diluent measured in advance relatively, calculates Hemoglobin Value.
Microcomputer 6 has the A/D converter 61 that the analogue signal that analogue signal processor 5 provides is converted to digital signal.The output valve of A/D converter 61 is transported to the exerciser 62 of microcomputer 6, calculates at exerciser 62, and light harvesting signal is carried out certain process.Exerciser 62 makes distributed data (two dimension scatterplot (unfiled) and one dimensional histograms) according to the output valve of detection part 4.
Microcomputer 6 includes the controller 63 being made up of control processor and control processor operation memorizer and the data analysis unit 64 being made up of the memorizer of analysis processor and the operation of analysis processor.Controller 63 is for controlling the instrument mechanical part 8 and other parts that are made up of the fluid system etc. for sample device (diagram is omitted), sample preparation and mensuration automatically supplying blood taking tube.Data analysis unit 64 for carrying out the analyzing and processing such as examination to each distributed data.Analyze result and be transported to the data processing equipment 3 of outside by interface 65, display data display and storage data etc. and process.
Microcomputer 6 has the interface 66 being connected with display operation part 7 and the interface 67 being connected with device for mechanical part 8.Exerciser 62, controller 63 and interface 66,67 are connected by bus 68, and controller 63 and data analysis unit 64 are connected by bus 69.Display operation part 7 on include operator assign start measure instruction start switch and display instrument state, various setting value and analyze result, accept operator input touch-screen type liquid crystal display.
Operation with regard to the cellanalyzer 1 of present embodiment below illustrates.Fig. 7 is the flow chart of the sample analyzer operation of present embodiment.User (operator) connects the power supply (step S1) of cellanalyzer 1, starts cellanalyzer 1.Self-inspection (step S2) is first carried out when this cellanalyzer 1 starts.In self-inspection, not only each ruuning situation running mechanical part of test microcomputer 6, inspection cellanalyzer 1, also it is measured the blank detection of the blank sample without specimen.Then, mode determination is carried out initially setting (step S3) by microcomputer 6.This initial set value is CBC+DIFF pattern.Specifically, in the process of step S3, set the parameter (service condition) of mensuration blood, such as reaction warehouse used and minute setting etc..So, the sample analyzer of present embodiment is with blood measuring pattern for initial launch pattern.Accordingly, cellanalyzer 1 is in the acceptable holding state starting and measuring.Microcomputer 6 shows the picture (step S4) of notice holding state on a liquid crystal display.
Under this holding state, operator can be operated by display operation part 7 and switch mode determination.Fig. 8 is the input image mode figure setting mode determination.This picture there are specimen number 120, specimen put into each display picture of schema category 121, subitem detection (mode determination) kind 122 and species of samples 123.Specimen is put into pattern and is provided with Three models: specimen container is manually inserted aspirating specimen mouth 18 and carries out the manual mode of aspirating specimen by operator；Specimen and reagent are mixed with and measure sample, aspirate the Trace Blood pre-dilution pattern of this mensuration sample with aspirating specimen mouth 18 by operator in advance；The closed mode of specimen is provided by the vehicle of automatic conveying specimen container.As species of samples, it is provided with normal blood specimen conventional (Normal), HPC(hemopoietic progenitor cell) specimen (HPC) and body fluid (BodyFluid).Operator can respectively specify that specimen puts into pattern, mode determination and species of samples.If operator specify blood measuring pattern, then species of samples is appointed as routine (Normal), then specifies any specimen to put into pattern and mode determination.If specifying humoral determination pattern, then operator specify " manual mode " respectively in putting into pattern, one of subitem detection is specified in " CBC+DIFF ", " CBC+DIFF+RET ", " CBC+DIFF+NRBC " and " CBC+DIFF+NRBC+RET ", appointment body fluid (BodyFluid) in species of samples.In step s 4, operator so specify desired mode determination.If operator do not change the mode determination of initial setting and carry out blood measuring (selecting N in step S5), then assign and start to measure instruction by starting switch.Microcomputer 6 receives and starts to measure instruction (step S6), aspirates blood preparation (step S7) from aspirating specimen mouth.
After blood preparation aspirates, specimen described above is imported into sampling valve 12, measures required sample (step S14) according to the subitem detection kind modulation of mode determination.The mensuration being then carried out measuring sample runs (step S16).Such as, when subitem detection kind is set as " 7 ", the various mensuration samples of HGB, WBC/BASO, DIFF, RET, NRBC, RBC/PLT are prepared.Then by white blood cell detection device 41, WBC/BASO, DIFF, RET, NRBC mensuration sample is measured, RBC/PLT detector 42 RBC/PLT mensuration sample is measured, HGB detector 43 HGB mensuration sample is measured.Now, white blood cell detection device 41 is provided only with one, and therefore, NRBC, WBC/BASO, DIFF, RET respectively measure sample and import white blood cell detection device 41 successively by the order of NRBC, WBC/BASO, DIFF, RET, measure item by item.In this mensuration is run, particle scattergram (scatterplot, rectangular histogram) drawn by exerciser 62.At this, just the process according to DIFF mensuration gained optical information drafting scatterplot illustrates.Exerciser 62, with the side scattered light in the light harvesting signal that exported by white blood cell detection device 41 in measuring at DIFF and lateral fluorescence signal for characteristic parameter, draws two dimension scatterplot (particle scattergram).This scatterplot (hereinafter referred to as DIFF scatterplot) is X-axis with lateral scattering light intensity, draws for Y-axis with lateral fluorescence intensity, and general " erythrocyte ghost population ", " lymph population ", " monokaryon population ", " neutrophilia+basophilia population " and " acidophil granules subgroup " occur.These population are identified by processing DIFF scatterplot by data analysis unit 64.
Then, it is analyzed processing (step S18) according to mensuration gained particle scattergram.At this in analyzing and processing, the data analysis unit 64 dialogue cell detector 41 of microcomputer 6 measures the DIFF scatterplot that when DIFF measures sample, exerciser 62 is drawn and is categorized as: four leukocyte groups (lymphocyte populations, mononuclear cell group, neutrophilia+basophil group and oxyphil cell group) and erythrocyte ghost group shown in Figure 12.In the analyzing and processing of present embodiment, the distance between each particle and the position of centre of gravity of each group that divide from scatterplot can obtain each particle degree of membership to each group.It is divided into each group according to each particle of these degree of membership.This method for classifying particles is documented on patent disclosure Heisei 5-149863 publication.Measure on gained scatterplot at WBC/BASO, be categorized as leukocyte group and erythrocyte ghost group beyond basophil group, basophil.Process, further according to DIFF Discrete point analysis, the result that leukocyte two is classified and counted by the result (with reference to Figure 12) leukocyte four classified and count and the process of WBC/BASO Discrete point analysis, leukocyte contained by blood preparation is carried out five classification.Specifically, data analysis unit 64 processes gained " blood cell count of neutrophil(e) cell+basophil " from DIFF Discrete point analysis and deducts WBC/BASO Discrete point analysis process gained " blood cell count of basophil ", can draw the blood cell count of neutrophil(e) cell and the blood cell count of basophil respectively.Accordingly, leukocyte is classified (lymphocyte, mononuclear cell, neutrophil(e) cell, basophil and oxyphil cell) by five, it is thus achieved that every blood cell count.Additionally, in RBC/PLT measures, the curve valley of the one dimensional histograms that the characteristic information that detection records according to detector 42 is drawn, erythrocyte and platelet are classified.The analysis result so obtained exports (step S20) on the display 302 of data processing equipment 3.
On the other hand, if microcomputer 6 is when the input that step S5 appointment mode determination received as described above is humoral determination pattern, it is set for the parameter (service condition) such as reaction warehouse used, minute setting etc. (step S8) of humoral determination.In the present embodiment, minute is three times during blood measuring as described later.
When mode determination is switched to humoral determination pattern by other mode determinations (in this case blood measuring pattern) (step S9), determinator 2 starts presequence and processes (step S10).Series processing is to prepare for humoral determination before this.What measure under humoral determination pattern is the specimen that hemocyte components and concentration is low, therefore, presequence process is carried out when being set as humoral determination pattern, to guarantee that background does not interfere with humoral determination result from blood measuring pattern (being shown as " 1: conventional " in fig. 8) switching.
Presequence processes and includes blank detection.Blank detection criterion in series processing before this is more tightened up than the criterion of the blank detection (carrying out after such as switch power supply and after automatically cleaning) carried out in determination of blood cell pattern, the value set as several points less than one.Additionally, when setting is switched to blood measuring pattern by humoral determination pattern, because background impact (impact of residue) does not generally involve blood measuring result, do not carry out so item presequence processes.When humoral determination pattern is repeatedly measured humoral specimen, because the typically not impact being subject to background, so not carrying out presequence process yet.But, humoral specimen also have containing a large amount of particles, therefore interface, when humoral specimen analyzes result beyond certain value, there will be " measurement result is too high, it is possible to impact specimen next time measures, and will carry out blank detection.Please by " confirmation "." etc. information, notify that operator likely affect following analyzing specimen result.Operator press " confirmation " button, can carry out blank detection.Now, interface is provided with " termination " button, as long as operator press " termination " button, it is also possible to does not carry out blank detection, moves to standby interface.If not carrying out blank detection, then preferably that measurement result Label reliability is low symbol.Do so adds blank detection when can only be defined in necessity, to prevent time and the waste of reagent class.
The presequence that Fig. 9 is mode determination to be implemented when switching to humoral determination pattern from blood measuring pattern processes the flow chart of step.Cellanalyzer 1 implements blank detection (step S31) by measuring blank sample at determinator 2, and measurement result is compared by microcomputer 6 with certain feasible value, it is judged that whether measurement result is lower than feasible value (step S32).When measurement result is lower than feasible value, microcomputer 6 terminates presequence and runs, Recovery processing.When measured value is more than feasible value, microcomputer 6 has judged whether to the blank detection (step S33) of stipulated number (such as three times), if blank detection number of times is not up to stipulated number, then returns step S31, is again carried out blank detection in above-mentioned stipulated number.If blank detection assay result is still not below feasible value in stipulated number, then in display operation part 7, shows blank determination result and include the picture (step S34) of " confirmation " button, " blank detection " button, " automatically cleaning " button.If operator press " confirmation " button (step S35), then microcomputer 6 terminates presequence operation, Recovery processing.If pressing " blank detection " button (step S36), then microcomputer 6 returns process to step S31 and again carries out blank detection.If pressing " automatically clean " button (step S37), then microcomputer 6 enforcement special cleaning automatically clean after (step S38), return process to step S31, again carry out blank detection.
After above-mentioned presequence process terminates, cellanalyzer 1 returns holding state (step S11).When operator start humoral determination, the same during with the manually mensuration of blood preparation, the aspirating specimen mouth 18 of determinator 2 is inserted the humoral specimen in specimen container, by starting switch.Microcomputer 6 is received (step S12) after starting mensuration instruction and is started to aspirate humoral specimen (step S13).
After humoral specimen aspirates, humoral specimen the same as blood preparation is imported into sampling valve 91.Prepared RBC/PLT by reaction warehouse 13 and measure sample (step S15).Then, measure DIFF and measure sample by white blood cell detection device 41, RBC/PLT detector 42 measure RBC/PLT and measure sample (step S17).When humoral determination pattern, white blood cell detection device 41 measure be only DIFF measure sample, therefore, even if minute is more longer than the minute of blood measuring pattern, it is also possible to than blood measuring time shorter time in complete measure.So, by the minute of humoral determination is extended longer than the minute of blood measuring, it is possible to improve the analysis precision of the low humoral specimen of particle concentration.Minute is more long, and the population of counting will be more many, and measurement accuracy will improve.But, minute is long, and sample disposal ability can decline, and will measure sample and be transported to syringe pump limited in one's ability of white blood cell detection device 41, therefore with 2 ~ 6 times for appropriate simultaneously.In the present embodiment, by humoral determination pattern minute time is set as 3 times during blood measuring pattern.
On the other hand, RBC/PLT measures sample and is introduced into resistive detectors 41 under any mode determination, is measured when certain a fluid stream.Then it is analyzed processing (step S19) according to mensuration gained characteristic information, analyzes result and export the display 302(step S21 of data processing equipment 3).In analyzing and processing under blood measuring pattern, analyze DIFF scatterplot etc., calculate the information (quantity and ratio) of five kinds of leukocyte subsets (neutrophil cell: NEUT, lymphocyte: LYMPH, mononuclear cell: MONO, acidophil: EO, basophilic leukocyte: BASO), but in the analyzing and processing under humoral determination pattern, because blood cell count seldom or is subject to breakage sometimes, therefore, with the formal classification of part integration for two kinds of subclass (mononuclear cell: MN, apocyte: PMN).Lymphocyte and mononuclear cell belong to mononuclear cell, and neutrophil(e) cell, oxyphil cell and basophil belong to apocyte.This sorting algorithm is identical with algorithm illustrated in the analyzing and processing under blood measuring pattern, and description will be omitted.
Then, the analysis result obtained in step S19 and feasible value (determined threshold values) are compared (step S22).This feasible value is same value with the feasible value of use in the blank detection in the presequence process of step S10.When analyzing result more than feasible value (step S22 selects "Yes"), then in step S23, show the confirmation interface 151 starting blank detection shown in Figure 17.This confirms to show on interface 151: " measurement result is too high, it is possible to affects following specimen and measures in display.Blank detection will be carried out.Please by " confirmation "." information display place 152 of information, ACK button 153 and cancel button 154.Next, it is judged that user's input is ACK button 153 or cancel button 154(step S24), if input is ACK button (in step s 24 choosing " confirmation "), then implement blank detection (step S25).When the analysis result obtained in step S19 is less than feasible value (selecting "No" in step S22) or input be cancel button time (in step s 24 choosing " cancellations "), then do not carry out blank detection, the process of return step S5.
Body fluid samples exists abnormal particle (macrophage and middle chrotoplast, tumor cell etc.) beyond hemocyte sometimes.The situation that there are these abnormal particles in cerebrospinal fluid is rarely found, but relatively common in other body fluid hydrothorax and ascites.Therefore, no matter body fluid kind how, accurately the hemocyte in body fluid will be classified and count it is necessary to get rid of the impact of these exception particles.Therefore, the present invention occurs in this new knowledge on the upside of the DIFF scatterplot of this cellanalyzer based on abnormal particle, enables instrument to measure more accurately as the leukocyte in the body fluid samples of target.This point does not account in aforesaid conventional art.
Figure 10 is the ideograph that present embodiment cellanalyzer 1 measured, analyzed the scatterplot that the DIFF mensuration sample prepared by body fluid and leukocyte mensuration reagent obtains under humoral determination pattern.The longitudinal axis of scatterplot represents lateral fluorescence intensity (more top, fluorescence intensity is more strong), and transverse axis represents lateral scattering light intensity (more keeping right, scattered light intensity is more strong).The region LF that the fluorescence intensity of scatterplot is weak is distributed the erythrocyte ghost Gc having haemolysis to produce, and the region HF distribution that fluorescence intensity is strong has the abnormal particles such as middle chrotoplast, and zone line MF distribution has monocyte Mc, multinuclear leucocyte Pc.Therefore, in the analysis of scatterplot, it is analyzed for leukocyte with the particle composition being distributed in the region MF except LF and the HF of region, is categorized as above-mentioned two classes, and counts.It addition, monocyte Mc comprises lymphocyte and mononuclear cell, multinuclear leucocyte Pc comprises neutrophil cell, oxyphil cell and basophil.
During leukocyte in such analysing body fluid, also contained blood cell count is little or impaired in body fluid sometimes, therefore as significant information clinically, is monocyte by leukocyte differential count and multinuclear leucocyte counts.
It addition, body fluid occasionally there are the abnormal particle (macrophage and middle chrotoplast, tumor cell etc.) beyond hemocyte.The situation that there are these abnormal particles in cerebrospinal fluid is rarely found, but relatively common in other body fluid hydrothorax and ascites.In the scatterplot of Figure 10, the nucleated cell beyond this leukocyte is distributed in region HF.In the present embodiment, it is possible to by the nucleated cell beyond leukocyte and leukocyte differentiation, therefore, even if body fluid also is able to obtain correct leukocyte count containing the nucleated cell beyond this leukocyte.By the cell occurring in region HF is counted, it is provided that the degree that abnormal cell occurs.In the present embodiment, according to the threshold values distinguishing each region, each cell is divided into region LF, MF and HF, it is also possible to this threshold values of manual change.
Figure 11 is the appropriateness for showing above-mentioned Discrete point analysis method, and compares the cellanalyzer 1 income analysis result adopting present embodiment and the accompanying drawing adopting counter point gained count results.Tested sample is hydrothorax, " this law " in figure represents the leukocyte count (WBC) and other abnormal populations (Others) that the cellanalyzer 1 of present embodiment calculates, and " Ref " represents the result that counter point (cell counting pond direct counting method (Fuchs-Rosenthal plate) and sitespin method) calculates.Example 1,2,3 all be analyze have abnormal particle to occur in a large number hydrothorax results, it can be seen that there is dependency relation between cellanalyzer 1 income analysis result and the counter point of present embodiment.
Figure 13 is that the analysis result as the above-mentioned DIFF mensuration sample prepared by blood is shown in the picture 100 on data processing equipment 3 display 302.The specimen viewing area of display specimen number 101 is arranged at the top of picture 100, and its side is provided with the attribute display district of display patient attribute.Attribute display district specifically shows specimen number, patient ID, patient's name, birthdate, sex, ward, the doctor in charge, mensuration date, minute and remarks etc..Bottom, attribute display district is provided with the measurement result viewing area of display measurement result.Measurement result viewing area is constituted by several pages, and these pages can by selecting several label 102 to show picture.Label has several for homepage, chart picture and sundry item.Figure 12 is the display picture during selection of chart label.The left-half of measurement result viewing area is provided with the measured value viewing area 103 of the measured value of display measurement result and the chart viewing area 104 of display chart, and right half part is provided with the scattergram viewing area 105 of the scattergram of display measurement result.The display of measured value viewing area project, data and the unit such as WBC, RBC ..., NEUT# ... BASO#, NEUT% ..., BASO%, chart viewing area 104 shows the labelling result can suspected about WBC, PLT, RBC or RET as the specimen exception of useful information in clinical examination and disease.
Scattergram viewing area 105 shows six scattergrams.The scatterplot of upper left quarter is DIFF scatterplot.Upper right quarter is WBC/BASO with, left portion is juvenile cell (IMI) use, and right middle is each scatterplot of RET.Lower left quarter is RBC rectangular histogram, and right lower quadrant is PLT rectangular histogram.
Figure 14 is that the measurement result as the above-mentioned DIFF mensuration sample prepared by body fluid is shown in the picture 110 on data processing equipment 3 display 302.The specimen viewing area 111 of display specimen number is arranged at the top of picture 110, and its side is provided with patient attribute viewing area.The left side of specimen viewing area 111 shows and represents " F " being measured with humoral determination pattern.Can being expressly understood that accordingly, this analyzes result is humoral determination result.Selected by available label 112 several pages of measurement result viewing area are constituted.In this example, have selected the label of " humoral determination (body fluid) ".
On measured value viewing area 113, the body fluid different from the measurement result of blood measuring pattern measures entry name WBC BF(WBC number), RBC BF(RBC number), MN#(mononuclear cell number (lymphocyte+unicellular)), PMN#(multi-nucleus cell number (neutrophil cell+basophil+acidophil)), mononuclear cell ratio in MN%(leukocyte), apocyte ratio in PMN%(leukocyte) and measured value, unit distinguish corresponding display.Also chart viewing area 114 it is again provided with blood measuring at humoral determination.Scattergram viewing area shows two distribution Figure 115, and top dispersion point diagram is DIFF scatterplot.Bottom is divided into RBC rectangular histogram.
Figure 15 for have selected in label 112 in Figure 14 picture 110 " retrieval BF(Research(BF)) illustration of label.This picture, except display search argument viewing area 116, also shows the project same with picture 110.Search argument viewing area 116 shows the ratio " HF-BF% " of the population in the population " HF-BF# " being present in region HF as shown in Figure 10, region HF and the population being present in the region including region HF and region MF, is present in the population " TC-BF# " in the region including region HF and region MF.Separately, " HF-BF% " is the ratio of HF-BF and TC-BF.
Figure 16 is the storage specimen guide look display picture 140 being shown on data processing equipment 3 display 302.130 is patient attribute viewing area.Its measurement result viewing area being arranged over being selected display measurement result by label.Measurement result viewing area leftmost column 131 is for showing that the checking work of measurement result is not done or done.V represents and has verified that.Its right row 132 are used for showing mode determination." F " represents the measurement result of humoral determination pattern.Although if needing the high level specimen of blank detection under humoral determination pattern, but not carrying out blank detection, in order to be showed, it is possible to by F inversion marks.
Above with regard to the 26S Proteasome Structure and Function of the cellanalyzer of the present invention, it is illustrated being previously charged into cellanalyzer, but this function can also be realized by control system, this control system is loaded traditional cellanalyzer, allows traditional cellanalyzer play the function of the present invention.
In structure described in present embodiment, under blood measuring pattern, leukocyte differential count is all the same with specimen amount time under humoral determination pattern to leukocyte differential count each formation determination sample, reagent type and amount of reagent.Can be not limited to this, it is also possible to allow the specimen amount preparing classification leucocyte mensuration sample under humoral determination pattern and amount of reagent respectively more than specimen amount and the amount of reagent of preparing classification leucocyte mensuration sample under blood measuring pattern.Due to leukocyte differential count minute is longer than blood measuring pattern under humoral determination pattern, measure required mensuration sample size also many, therefore, do so the leukocyte differential count under blood measuring pattern and the leukocyte differential count under humoral determination pattern can prepare appropriate mensuration sample respectively.
In the present embodiment, just under humoral determination pattern, the structure of leukocyte differential count is set forth with scattering light and fluorescence, but is not limited to this, it is also possible to use such as scattering light and absorbing light to leukocyte differential count under humoral determination pattern.The stain of stain leukocytes can be mixed into specimen by light absorbing mensuration together with other reagent, formation determination sample, is supplied to flow cell by this mensuration sample, so as to form sample stream in flow cell, this sample stream of illumination, receives, by light collecting elements such as photodiodes, the light that sample stream sends.Leukocyte is by time in flow cell, and light is absorbed by leukocyte, and its degree of absorption can be caught as the light harvesting amount of light collecting element.About this light absorbing mensuration, U.S. Patent No. 5122453 and delivering on No. 5138181 publications of U.S. Patent No..Also can measure resistance and replace scattering light, by resistance value and absorbing light, leukocyte be classified.
Claims (9)
1. a cellanalyzer, including:
Fluid device, it has an aspirating specimen mouth aspirating specimen, and mixing aspirates described specimen that mouth aspirates and reagent carrys out formation determination sample by described；
Detection part, the scattering light of its cell acquisition being capable of detecting when from the described mensuration sample flowing through sheath flow pool and fluorescence；
Controller, it processes the measurement result from described detection part output and obtains analysis result；And
Display；
Wherein, described controller makes described display show for accepting the input picture to blood measuring pattern and the selection of humoral determination pattern；
If selected for described blood measuring pattern, then the pattern of putting into of specimen at least can specify following pattern: specimen container is manually inserted described aspirating specimen mouth and aspirates the 1st pattern of specimen and supply specimen by the vehicle of specimen container described in automatic conveying and aspirated the 2nd pattern of specimen by described aspirating specimen mouth by operator；
If selected for described humoral determination pattern, then described 1st pattern can only be specified to put into pattern as specimen.
2. cellanalyzer according to claim 1, it is characterised in that:
If selected for described blood measuring pattern, then the pattern of putting into of specimen can also specify following pattern: aspirate the prior compound sample of operator and reagent by aspirating specimen mouth and prepare measure sample the 3rd pattern.
3. cellanalyzer according to claim 1, it is characterised in that:
Described controller makes described display show the input picture that can select that the pattern of putting into of specimen, mode determination and species of samples；
In described input picture, it is possible to select blood and body fluid as species of samples.
4. cellanalyzer according to claim 3, it is characterised in that:
In described input picture, described blood measuring pattern is set to initial launch pattern.
5. cellanalyzer according to claim 1, it is characterised in that:
Aspirate humoral specimen if selected for described humoral determination pattern and by described 1st pattern, then described fluid device mixes described humoral specimen and described reagent carrys out formation determination sample automatically.
6. cellanalyzer according to claim 1, it is characterised in that:
After have selected described humoral determination pattern described fluid device prepare the reagent used when leukocyte differential count measures sample and after have selected described blood measuring pattern described fluid device prepare leukocyte differential count to measure the reagent of use during sample be same.
7. cellanalyzer according to claim 6, it is characterised in that:
Described leukocyte differential count reagent comprises the surfactant of lysed erythrocyte.
8. cellanalyzer according to claim 6, it is characterised in that:
Described leukocyte differential count reagent comprises polymethine class pigment.
9. cellanalyzer according to claim 1, it is characterised in that:
After switching to described humoral determination pattern from described blood measuring pattern, described controller is measured the blank detection of blank sample.
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| JP2007-119012 | 2007-04-27 | ||
| CN200810005239.2A CN101236195B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and control system thereof |
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| CN201610209662.9A Active CN105807038B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and its control system |
| CN201610208992.6A Active CN105807037B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and its control system |
| CN200810005239.2A Active CN101236195B (en) | 2007-02-01 | 2008-01-31 | Cellanalyzer, method for analyzing body fluid and control system thereof |
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Also Published As
| Publication number | Publication date |
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| CN105891090B (en) | 2020-01-17 |
| JP2008209386A (en) | 2008-09-11 |
| CN105866012A (en) | 2016-08-17 |
| CN105891090A (en) | 2016-08-24 |
| CN105807037B (en) | 2019-05-28 |
| CN101236195A (en) | 2008-08-06 |
| JP4926812B2 (en) | 2012-05-09 |
| CN105807037A (en) | 2016-07-27 |
| CN101236195B (en) | 2016-05-04 |
| CN105807038B (en) | 2019-06-18 |
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