CN105784443A - Sample detection device, and sample collection and detection device and method - Google Patents
Sample detection device, and sample collection and detection device and method Download PDFInfo
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- CN105784443A CN105784443A CN201610153236.8A CN201610153236A CN105784443A CN 105784443 A CN105784443 A CN 105784443A CN 201610153236 A CN201610153236 A CN 201610153236A CN 105784443 A CN105784443 A CN 105784443A
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Instruments for taking body samples for diagnostic purposes; Other methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determination; Throat striking implements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
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- Physics & Mathematics (AREA)
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- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
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- Microbiology (AREA)
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- Hydrology & Water Resources (AREA)
- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to a sample detection device, and a sample collection and detection device and method. The sample detection device comprises a detection cavity and also comprises a sample reaction cavity, a passage being provided between the detection cavity and the sample reaction cavity; the passage is closed in the initial state so that the detection cavity is not in liquid communication with the sample reaction cavity; the passage is opened in the detection state so that the detection cavity is in liquid communication with the sample reaction cavity. The device and method of the invention enable collection, buffered reaction and detection of a liquid sample to be completed in a one-station form, take a certain period of pretreatment detection particularly for the sample, and enable the detection to be done within a set time.
Description
Technical field
The present invention relates to a kind of sample testing apparatus, especially for collecting and detect the collection and detection device of sample and collection and detecting the method for analyte in liquid sample.
Background technology
Following background technology is adapted to assist in the reader understanding present invention, and is not construed as prior art.
Utilize immunity association reaction principle to detect this technology that whether there is analyte in sample and be widely used in every field.The analyte of various biological specimen (saliva, blood, urine, serum, perspiration etc.) can be detected with it and carry out the health status (early pregnancy, tumor, infectious disease, drugs etc.) of monitoring of diseases and the mankind.The cardinal principle of this detection technique is built upon between immune molecule having the performance of specific bond, for instance antibody and antigen, hapten/antibody, biotin and antibiotin etc..
At medical diagnostic field, utilize detecting device or detection cup not to collect detection liquid sample, and judge whether liquid sample contains analyte, be a kind of commonplace method.Such detecting device or detection cup generally require that sample is collected in sample container, and person skilled is drawn together into a detectable bar and makes a part of submergence of reagent strip in the sample, take out reagent strip and read testing result after the some time.
US Patent No. 2004/0184954 and US2004/0237674 disclose some and collect saliva and carry out whether containing in detection saliva the device of illicit drugs composition.In the two patent, both provide the apparatus and method collected and detect saliva, in these devices, after sample is sampled on catcher, by applying external force, the sample in absorption piece on catcher is expressed in collecting chamber, then detects again.But, applicator is coordinating in extrusion process with collecting chamber, it is possible to has sample contact and pollutes to operator or infect；Further, when running into sample and needing first and detect after buffer mixing again, such collection device just seems not very convenient.
It addition, in some detections, sample needs to carry out processing and can be detected.Such as, add buffer solution in the sample, make sample be diluted reducing viscosity, it is ensured that sample can dialysis etc. smoothly on detector bar.Buffer solution how can be made to mix smoothly with sample, and mixing fully and conveniently operates the problem being all to need to solve.
Summary of the invention
The invention provides the detecting device of a kind of novel sample and sample collection detects the device integrated, sample can be made to realize collection and pretreatment and realize the function of detection by this device.
Specifically, on the one hand, providing a kind of sample testing apparatus in the present invention, including test chamber, wherein, described sample testing apparatus also includes sample reaction chamber, is provided with a passage between test chamber and sample reaction chamber；Described passage is closed when original state and is made test chamber and sample reaction chamber liquid be not attached to lead to；Described passage is opened when the state of detection and is made test chamber and sample reaction chamber liquid communication.
Some preferred embodiment in, described sample reaction chamber is connected in test chamber, and the bottom of described sample reaction chamber is connected to the sample receiving terminal of test chamber；Described passage is be positioned at the through hole being connected bottom sample reaction chamber with test chamber；During original state, through hole is sealed；During detection state, the through hole of sealing is unlocked.
Some preferred embodiment in, described through hole be provided with seal through hole sealing-plug.
Some preferred embodiment in, described sample testing apparatus also includes lid, and described lid is used for covering and is positioned at sample reaction chamber；Lid end is connected to raised line.
Some preferred embodiment in, after lid covers sample reaction chamber, raised line is positioned at directly over reaction chamber inner via hole.That is, the position that raised line is on lid more corresponding with the lead to the hole site bottom reaction chamber preferred embodiment in, sample testing apparatus also includes ring-type buckle, and buckle is fixed on the sidewall of lid top；Buckle center is provided with projection, covered body wall has and holds protruding shrinkage pool, and after described buckle projection snap-on caps body sidewall shrinkage pool, lid and buckle interfix.More specifically in embodiment, projection is positioned at the center, medial wall of buckle.
Some preferred embodiment in, the accent of described sample reaction chamber is provided with groove, is used for receiving and fixing buckle.
Some preferred embodiment in, lid has primary importance and the second position on sample reaction chamber；During original state, when lid is positioned at primary importance, buckle is fastened between lid and sample reaction chamber and fixing lid, and lid top not contacting reaction cavity accent, the raised line on lid does not contact the through hole plug on sample reaction chamber bottom through-hole；During detection state, when lid is positioned at the second position, buckle is removed, lid directly covers after being pressed down on sample reaction chamber, the direct contacting reaction cavity accent in lid top, the raised line on lid contacts and pokes the through hole plug bottom sample reaction chamber, makes sample reaction chamber be connected with test chamber.
Some preferred embodiment in, lid end is additionally provided with draw-in groove；In draw-in groove, engaging has chip carrier.
Some preferred embodiment in, lid has pore.This pore can make sample reaction chamber keep gas to be connected with extraneous, so that the air pressure of sample reaction chamber is identical with atmospheric pressure.In one specific embodiment, the aperture of described pore is less, generally between 0.5-2mm.
Some preferred embodiment in, include one or more testing element in test chamber；The sample region of acceptance of described testing element is positioned at the lower section of sample reaction chamber bottom through-hole.
On the other hand, the present invention also provides for a kind of sample collection detecting device, including test chamber, it is characterised in that also include sample reaction chamber, collection well；The bottom of described sample reaction chamber is connected to the sample receiving terminal of test chamber, and described sample reaction chamber is connected with collection well and connects；Being provided with a passage between test chamber and sample reaction chamber, described passage is be positioned at the through hole being connected bottom sample reaction chamber with test chamber；During original state, through hole is sealed；During detection state, the through hole of sealing is unlocked.
Some preferred embodiment in, described collection well is positioned at one end of test chamber；The bottom of described collection well is connected with sample reaction chamber sidewall；There is bottom collecting chamber the through hole connected with sample chamber sidewall.
Some preferred embodiment in, also include the lid covering sample reaction chamber；The end of lid is connected to raised line.
Some preferred embodiment in, also include ring-type buckle, buckle is fixed on covered body wall；Buckle center is provided with projection, covered body wall has and holds protruding shrinkage pool, and after described buckle projection snap-on caps body sidewall shrinkage pool, lid and buckle interfix.
Some preferred embodiment in, lid has primary importance and the second position on sample reaction chamber；During original state, when lid is positioned at primary importance, buckle is fastened between lid and sample reaction chamber and fixing lid, and the raised line on lid does not contact the through hole plug of sample reaction chamber sealed bottom through hole；During detection state, when lid is positioned at the second position, buckle is removed, and lid directly covers after being pressed down on sample reaction chamber, and the raised line on lid contacts and poke the through hole plug bottom sample reaction chamber, makes sample reaction chamber be connected with test chamber.When described device is by original state to the process of detection state, namely lid is by primary importance to the process of the second position, raised line on lid moves to bottom in reaction chamber, by initial not contact through hole plug to contact through hole plug, then to pushing and poking through hole plug.
Some preferred embodiment in, also include sample collection rod, described sample collection rod includes the absorption piece and the handle that are connected；And the seal member between absorption piece and handle.
Some preferred embodiment in, collection well accent place be provided with symmetry two hole clippings；The one end being connected with absorption piece on sample collection bat handle is provided with two card articles of symmetry.
After sample collection rod insertion collecting chamber carries out sample collection, after absorption piece on sample collection rod is extruded completely, on handle, card article then can arrive the hole clipping place at collecting chamber accent place, and card article and hole clipping are fastened togather, and make collection rod be locked in collecting chamber, so that the absorption piece on collection rod is constantly in compressive state, what ensure sample fully extrudes outflow, and, sample collection rod is locked in collecting chamber, will not be randomly placed, cause environmental pollution.
In some embodiments, the sample collection detecting device of the present invention, including testing element, testing element includes sample region of acceptance, reagent area and detection zone, it is characterized in that, also including test chamber, described test chamber includes base plate and upper plate, base plate and upper plate engaging form the test chamber of hollow, and described testing element is positioned at test chamber；Described upper plate is connected to the sample reaction chamber of the one end open enclosed by sidewall and bottom, wherein, is a part for upper plate bottom sample reaction chamber；A through hole it is provided with bottom described sample reaction chamber；Described through hole is positioned at the top of the sample region of acceptance of testing element.
Some preferred embodiment in, described through hole be provided with seal through hole through hole plug；During original state, through hole is sealed by through hole plug；During detection state, through hole plug is opened and is made through hole be unlocked.During original state, through hole is sealed by through hole plug and makes test chamber and sample reaction chamber liquid be not attached to lead to；During detection state, through hole plug is opened and is made through hole be unlocked to make test chamber and sample reaction chamber liquid communication.
Some preferred embodiment in, the collection well of the described one end open also including being enclosed by sidewall and bottom, collection well is positioned at one end of test chamber upper plate adjacent sample reaction chamber.
Some preferred embodiment in, described reaction chamber, collecting chamber and test chamber upper plate are integral type structure.
Some preferred embodiment in, described collection well is connected with sample reaction chamber and liquid communication.
Some preferred embodiment in, described collection well sidewall is connected with test chamber upper plate.
One preferred embodiment in, reaction chamber is vertically positioned on upper plate；Described collection well is laterally positioned on upper plate.Some preferred embodiment in, the bottom of described collection well is connected with the sidewall of reaction chamber；There is bottom described collection well the through hole being connected with reaction chamber.
In the present invention, cleverly collecting chamber and reaction chamber are fully integrated on the upper plate of test chamber, and collecting chamber and reaction chamber also integrate, make whole sample collection detecting device moulding light and handy, and enabling sample to collect, pretreatment and detection integral type complete, convenient to operation.Concrete, in order to easy to operate, good looking appearance, collecting chamber and reaction chamber are mutually perpendicular to, namely reaction chamber is vertically positioned on test chamber upper plate, and collecting chamber laterally crouches at test chamber upper plate, namely, the bottom of collecting chamber and the sidewall of reaction chamber link together, and the bottom of reaction chamber is positioned at the top of sample application zone of testing element.
Some preferred embodiment in, also include lid, described lid is used for covering and is positioned at sample reaction chamber；Lid end is connected to raised line.
Some preferred embodiment in, also include ring-type buckle, buckle is fixed on the sidewall of lid top；Buckle center is provided with projection, covered body wall has and holds protruding shrinkage pool, and after described buckle projection snap-on caps body sidewall shrinkage pool, lid and buckle interfix.
Some preferred embodiment in, the accent of described sample reaction chamber is provided with groove, is used for receiving and fixing buckle.Buckle is fixed on sample reaction chamber, and the lid interfixed with buckle is also fixed in the position of sample reaction chamber, even if also the raised line of lid end is fixed on the surface of through hole plug.
Some preferred embodiment in, lid has primary importance and the second position on sample reaction chamber；During original state, when lid is positioned at primary importance, buckle is fastened between lid and sample reaction chamber and fixing lid, and lid top not contacting reaction cavity accent, the raised line on lid does not contact the through hole plug on sample reaction chamber bottom through-hole；During detection state, when lid is positioned at the second position, buckle is removed, lid directly covers after being pressed down on sample reaction chamber, the direct contacting reaction cavity accent in lid top, the raised line on lid contacts and pokes the through hole plug bottom sample reaction chamber, makes sample reaction chamber be connected with test chamber.
Some preferred embodiment in, lid end is additionally provided with draw-in groove；In draw-in groove, engaging has chip carrier.
Some preferred embodiment in, lid has pore.This pore can make sample reaction chamber keep gas to be connected with extraneous, so that the air pressure of sample reaction chamber is identical with atmospheric pressure.In one specific embodiment, the aperture of described pore is less, generally between 0.5-2mm.
Some preferred embodiment in, also include sample collection rod, described sample collection rod includes the absorption piece and the handle that are connected；And the seal member between absorption piece and handle.
Some preferred embodiment in, collection well accent place be provided with symmetry two hole clippings；The one end being connected with absorption piece on sample collection bat handle is provided with two card articles of symmetry.
In addition, the present invention also provides for a kind of method collecting and detect liquid sample, for analyzing the analyte in detection liquid sample, wherein, including a sample collection detecting device collecting and detecting analyte in liquid sample, this sample collection detecting device includes test chamber, sample reaction chamber, collection well；Described sample reaction chamber is connected with collection well and connects；Being provided with a passage between test chamber and sample reaction chamber, described passage is positioned at the bottom of the sample reaction chamber that the sample receiving terminal with test chamber is connected, and described passage is through hole；During original state, through hole is sealed；During detection state, the through hole of sealing is unlocked；Described sample collection detecting device also includes the lid covering sample reaction chamber, and be fixed the buckle on lid, and sample collection rod；Described specifically comprise the following steps that
(1) sample collection detecting device is original state, and namely lid is fixed on the primary importance of sample reaction chamber by buckle, the through hole plug of the raised line not contacting reaction cavity bottom through-hole that lid end connects；
(2) insert collecting the sample collection rod having liquid sample in collection well, and make collecting chamber be collected rod sealing；
(3) continue to advance sample collection rod bottom collecting chamber, make the liquid on sample collection rod be flowed in collecting chamber and be flowed in sample reaction chamber by the through hole connected with the sidewall of sample reaction chamber bottom collection well；
(4) making the card article on collection rod be engaged in the hole clipping at collection well accent place makes collection rod be locked in collecting chamber；
(5) stand the regular hour, make the liquid sample in sample reaction chamber react with the material in reaction chamber；
(6) buckle being fastened on lid is taken off, the pore at closure body center, press down on lid, lid is made to be covered to the accent of sample reaction chamber, namely lid is positioned at the second position of reaction chamber, now, sample collection detecting device is in detection state, and the end being positioned at the raised line of lid inner top contacts and poke the through hole sealed bottom sample reaction chamber；
(7) liquid sample enters test chamber, engaged test element by the through hole being punctured, and carries out the detection of analyte in liquid sample.
Some preferred embodiment in, the material in reaction chamber is the reagent being loaded on carrier；Carrier is fixed in reaction chamber by described one end by the draw-in groove engaging carrier on lid.
In the present invention, after sample enters reaction chamber, in order to allow sample have time enough to react with the material in reaction chamber or reagent, make sample can be carried out pretreatment fully, generally, device can be allowed to stand the regular hour, such as 1-5 minute, 1 minute, 2 minutes or 3 minutes etc..It is of course also possible to make sample enter after reaction chamber carries out pretreatment, not anxious detecting, therefore, this device can be longer in the regular hour that detection please stand, as long as generally can more than 1 minute, such as the time of 1 minute to 1 day can.
Beneficial effect
Apparatus and method of the present invention can make the one-stop of the collection of liquid sample, buffering reaction and detection complete, and needs to carry out the detection of certain time pretreatment especially for sample, and the time setting that is capable of is detected.
Accompanying drawing explanation
Fig. 1 is the decomposing schematic representation of the sample collection detecting device of the present invention；
Fig. 2 is the generalized section of the sample collection detecting device of the present invention；
Fig. 3 to Fig. 6 is the sample collection of the sample collection detecting device of the present invention the operating process schematic diagram detected；Wherein:
Fig. 3 is the sample collection detecting device schematic diagram with collecting part released state of the present invention；
The collecting part that Fig. 4 is the present invention compresses the schematic diagram being combined in sample collection detecting device (lid is in primary importance)；
Fig. 5 is the schematic diagram that the buckle of the present invention separates with sample collection detecting device；
Fig. 6 is the sample collection detecting device schematic diagram of (lid is in the second position) after the lid of the present invention is pressed down；
Fig. 7 to Figure 10 is the sample collection of the sample collection detecting device of the present invention the operating process generalized section detected: wherein:
Fig. 7 is the sample collection detecting device generalized section with collecting part released state of the present invention；
The collecting part that Fig. 8 is the present invention compresses the generalized section being combined in sample collection detecting device (lid is in primary importance)；
Fig. 9 is the generalized section that the buckle of the present invention separates with sample collection detecting device；
Figure 10 is the sample collection detecting device generalized section of (lid is in the second position) after the lid of the present invention is pressed down.
Description of reference numerals:
Sample collection detecting device 800；Test chamber 100；Base plate 102；Upper plate 101；Window 103；Testing element 200；Sample reception district 201；Sample reaction chamber 300；The accent 303 of sample reaction chamber；Bottom sample reaction chamber 304；Reaction chamber sidewall 305；Through hole 301 bottom reaction chamber；Reaction chamber accent groove 302；Through hole plug 310；Lid 400；Lid top 450；Lid end 460；Lid shrinkage pool 401；Carrier 410；Raised line 420；Pore 430；Collection well 500；The through hole 510 connected with reaction chamber bottom collecting chamber；Collecting chamber accent 520, collecting chamber sidewall 530；Bottom collecting chamber 540；Hole clipping 501；Buckle 600；Buckle projection 601；Sample collection rod 700；Collecting part 701；Handle 702；Sealing ring 703；Card article 704；Salient point 705 on card article；
Detailed description of the invention
Below the structure that the present invention relates to or these technical terms used are described further.
Detection
Detection represents whether chemical examination or a kind of material of test or material exist, such as, but it is not limited to this, chemical substance, organic compound, inorganic compound, metabolism product, medicine or the metabolite of drug metabolite, organic organization or organic organization, nucleic acid, protein or polymer.It addition, detection represents the quantity of test substances or material.Furtherly, chemical examination is also represented by immune detection, chemical detection, enzyme detection etc..
Sample
The sample of indication of the present invention refers to that those can be used to detect, chemically examine or diagnose the material that whether there is analyte interested.It may be that such as, liquid sample, liquid sample can include blood, blood plasma, serum, urine, saliva and various juice to sample, it is also possible to includes the liquid solution that solid sample and semi-solid samples are formed after anticipating.The sample that collection comes may be used for the methods such as immune detection, chemical detection, enzyme detection and detects whether there is analyte.
Analyte
Any analyte can be analyzed by apparatus and method of the present invention.Analyte can detect in any liquid or liquefied sample, for instance urine, saliva, saliva, blood, blood plasma, or serum.Analyte can also is that some hapten materials, and these hapten include drugs (such as Drug abuse)." Drug abuse " (DOA) refers to that non-medical destination uses medicine (generally playing the effect that paralysis is neural).This device is used to can be used for detecting and belong to medical usage but the detection of easy drug overdose, such as tricyclic antidepressant (imipramine or the like) and acetyl aminophenol etc..These medicines can resolve into different small-molecule substances after being absorbed by the body, and these small-molecule substances are present in the body fluid such as blood, urine, saliva, sweat or part body fluid exists above-mentioned small-molecule substance.
Testing element 200
Testing element 200 can select the test strip of horizontal mobility, and it can detect multiple analyte.Certainly, other suitable testing elements can also be used in the present invention.Various testing elements can be grouped together and apply in the present invention.A kind of form is Test paper.Can be various forms for analyzing the Test paper of the analyte (such as drugs or the metabolite showing health) in sample, such as immunoassay or chemico-analytic form.Test paper can adopt the analytical model of non-competing method or competition law.Test paper comprises an absorbent material with sample region of acceptance, reagent area and test section.Add sample to sample region of acceptance 201, by capillarity stream to reagent area.In reagent area, if there is analyte, sample is combined with reagent.Then sample continues to flow to detection zone.Other reagent, as being fixed on detection zone with the molecule of the Non-specific combination of analyte.These reagent react and are combined in by analyte this district with the analyte (if existence) in sample, or are combined with some reagent of reagent area.Label for showing detection signal exists and reagent area or the mark zone that separates.
Typical non-competing method analytical model is: if containing analyte in sample, signal will produce, if not comprising analyte, does not just produce signal.In competition law, if analyte is not present in sample, signal produces, if there is analyte, does not then produce signal.
Testing element 200 is Test paper, it is possible to select water suction or the material not absorbed water.Test paper can include multiple material and transmit for liquid sample.The material of one of which Test paper can cover on another material, as filter paper covers on nitrocellulose filter.One district of Test paper can select one or more materials, and another district selects one or more materials that other are different.Test paper can be attached on certain holder or hard surface for improving the intensity being affectedly bashful Test paper.Analyte is detected by signal generating system, as utilized one or more enzymes with this analyte generation specific reaction, utilize as aforementioned method specific combinating substance being fixed on Test paper, the compositions of one or more signal generating systems is fixed on the analyte detection zone of Test paper.The material producing signal can at sample region of acceptance 201, reagent area, or detection zone, or on whole Test paper, this material can be full of on one or more materials of Test paper.Solution containing signal thing is added to the surface of reagent paper or one or more materials of reagent paper are immersed in the solution containing signal thing.Addition is made to dry containing the reagent paper of signal thing solution.
Each district of Test paper can arrange in the following manner: sample region of acceptance 201, reagent area, detection zone, control zone, it is determined that the whether adulterated district of sample, liquid sample uptake zone.After control zone is positioned at detection zone.All of district can be arranged at only with on a reagent paper of a kind of material.May also be not same district and adopt different materials.Each district can directly contact with liquid sample, or different districts is according to the direction arrangement of liquid sample flowing, is connected with the front end in another district by the end in each district and overlaps.Material used can be water absorption good material such as filter paper, glass or nitrocellulose filter etc..Test paper can also adopt other forms.
Sample collection rod 700
Generally, sample collection rod includes absorption piece 701 and handle 702, and absorption piece 701 is positioned at one end of handle 702.Absorption piece is generally made up of the other sponge of medical grade commonly used in the art or plastic foam material.But many other materials may be made as absorption piece, for instance Cotton Gossypii or paper, or other any materials with water absorbing properties.Handle is usually rigidity, is conducive to the operation to absorption piece.Handle can be made up of material commonly used in the art, for instance plastics, timber, metal or cardboard.In certain embodiments, also having O-ring seals 703 in one end that handle is connected with absorption piece, this sealing ring is surrounded on the junction of handle and absorption piece.For seal accept collect rod collecting chamber, make the liquid sample being located therein will not reflux to handle.In some modes being more highly preferred to, more thorough in order to what make the liquid in absorption piece be extruded, simultaneously, also avoiding sample collection rod to be randomly placed, be provided with the card article 704 of symmetry on sample collection rod, these card articles have elasticity, compressed after entering collecting chamber, after arriving certain position, the salient point 705 on card article touches and is released after hole clipping on collecting chamber sidewall, and salient point enters in hole clipping engaged fixing.
Test chamber 100
Test chamber 100 is generally a cavity, is used for holding testing element 200, and enables liquid sample to enter engaged test element in this cavity to detect.Its various shapes, it is possible to shape and the quantity of the testing element held as required are designed.In the present invention, testing element is test strips, therefore, in an embodiment, test chamber is plank frame, having base plate 102 and upper plate 101, the two forms a cavity 100 after being combined by the mode engaged, more specifically, after test strips 200 is placed on base plate 102, cover upper plate 101, upper plate 101 is fixed on base plate 102, form test chamber 100.More specifically, base plate 102 having the strip-shaped clamp groove of one or more projection, these draw-in grooves are used for placing and fixing test strips 200.In other limited embodiments, having a window 103 on upper plate, window 103 position is corresponding with the detection zone of test strips, the convenient test result observing test strips.
Sample reaction chamber300
In some detection, sample needs to be processed in advance before detected, such as, it is diluted, or needing to react with a certain reagent in advance and make analyte therein be activated or be labeled, therefore sample reaction chamber 300 is while storing liquid sample, makes liquid sample realize mixing with buffer solution in this cavity, or make a certain reagent and liquid sample carry out pre-reaction so that liquid sample is pretreated in sample reaction chamber.In specific embodiment, sample reaction chamber 200 is enclosed the tubular structure of hollow by bottom and sidewall.
In the following detailed description, the subsidiary reference word of legend is a part here, and it is to illustrate that the present invention can illustrate in the way of actable specific concrete scheme.We are not precluded from the present invention and can also carry out other concrete scheme and change the structure of the present invention when without prejudice to the use scope of the present invention.
First, the sample testing apparatus of the present invention mainly includes sample reaction chamber 300 and test chamber 100.Wherein, test chamber 100 is used for disposing testing element 100, receives liquid sample simultaneously, makes the liquid sample of entrance test chamber 100 sequentially flow through testing element 200, it is achieved detection.And sample reaction chamber 300, it is used for storing liquid sample, and makes liquid sample realize mixing with buffer solution in this cavity 300, or make a certain reagent and liquid sample carry out pre-reaction so that liquid sample is pretreated in sample reaction chamber 300.In order to realize detection function, test chamber 100 and sample reaction chamber 300 also need to liquid phase circulation, i.e. making sample react, the liquid fluid in 300 is in test chamber 100 and arrives in test strips 200.Therefore, a passage it is additionally provided with between test chamber 100 and reaction chamber 300.Because liquid sample needs to process in advance, and not pretreated sample can not detect, so, this passage can not make reaction chamber 300 and test chamber 300 be constantly in connected state, test chamber 100 and sample reaction chamber 300 liquid is made to be not attached to lead to it is to say, this passage is closed when original state；Passage is opened when the state of detection and is made test chamber 100 and sample reaction chamber 300 liquid communication.Therefore, how to realize between test chamber 100 and reaction chamber 300 liquid when needs detect just to circulate and become crucial.
In the present invention, as shown in Figure 1, test chamber 100 is mutually clamped is formed by base plate 102 and upper plate 101, test strips 200 is fixed in test chamber 100 between base plate 102 and upper plate 101, corresponding position above test strips sample application zone 201, upper plate 101 is connected to a cavity 300, and this cavity 300 is enclosed formed by sidewall 305 and bottom 304, there is opening 303, be also a part for test chamber upper plate 101 bottom cavity 300；This cavity 300 is columnar structured, is vertically positioned on test chamber upper plate 101, as shown in Figure 2.Being provided with a through hole 301 bottom reaction chamber 300, this through hole 301 makes to connect between reaction chamber 300 with test chamber 100, and more specifically, through hole 301 is positioned at the top of the sample region of acceptance 201 of testing element；When original state, through hole 301 is sealed, and when needs detect, makes through hole 301 be unlocked and makes to connect between reaction chamber 300 with test chamber 100.The mode that through hole 301 is sealed is not limit, opening when needed, and chemically can not on the material of the detection generation impact of liquid sample, therefore, diaphragm seal can be used, it is possible to use various sealing-plugs etc., in a specific embodiment, utilizing through hole plug 310 to seal through hole, the material of through hole plug is silica gel.Before sample is detected, through hole plug 310 is positioned at through hole and seals through hole, and the liquid sample in reaction chamber 300 can not arrive test chamber 100, after band sample is pretreated or when needing sample is detected, through hole 301 is opened, namely takes off through hole plug 310 or make through hole plug 310 by perforate.In some specific embodiments, in order to easy to operate, through hole plug 310 makes through hole open by the mode being punctured.
More specifically in embodiment, this detecting device also includes a lid covered on sample reaction chamber 300 400, and is fixedly connected on the raised line 420 on lid, pokes or puncture through hole plug 310 by raised line 420.This raised line 420 is connected to the end 460 of lid 400, when raised line 420 is positioned at reaction chamber 300, raised line 420 is positioned at the surface of through hole plug 310, in order to make raised line 420 can poke through hole plug 310 when needed, raised line 420 length be a bit larger tham or equal to the height of reaction chamber 300.Simultaneously, just in order to make raised line 420 puncture through hole plug 310 when needed, need raised line 420 not contact through hole plug 310 when initial position, in one embodiment, lid 400 is also surrounded with a ring-type buckle 600, this ring-type buckle 600 is positioned at the top 450 of lid 400, after lid 400 covers reaction chamber 300, buckle 600 is between the accent 303 of lid top 450 and reaction chamber 300, lid top 450 is made not contact with reaction chamber accent 303, as shown in Figure 3 and Figure 4, also the end not contact through hole plug 310 of the raised line 420 being connected on lid is made, as shown in Figure 7.When initial position, namely when lid 400 is positioned at primary importance, buckle 600 is fastened between lid top 450 and reaction chamber accent 303, is positioned at the raised line 420 not contact through hole plug 310 of reaction chamber 300；When needs detect, take off buckle 600, as shown in Figure 5, lid 400 is made to be moved downward to lid top 450 contacting reaction cavity accent 303, as shown in Figure 6, namely lid 400 arrives the second position, in this process, along with the end of the projection 420 moved down together of lid 400 contacts and puncture through hole plug 310, as shown in Figure 10.In some embodiments, in order to make raised line 420 can be positioned at the surface of through hole plug 310 accurately, make raised line 420 can poke through hole plug 310 smoothly, need the fixing lid 400 position on reaction chamber 300 so that the position of raised line 420 is fixed, lid 600 is fixed by buckle 600, so that lid 400 can not on reaction chamber 300 random change of location, more specifically, the center position of ring-type buckle 600 has protruding 601, the sidewall corresponding position at lid top 450 has shrinkage pool 401, after buckle protruding 601 inserts covered body wall shrinkage pool 401, position between buckle 600 and lid 400 interfixes.One, more specifically in embodiment, on the accent 303 of reaction chamber, also has a groove 302, and this groove 302 is for placing a part for annular buckle 600, and more specifically, the part of buckle projection 601 junction is positioned at the groove 302 of reaction chamber accent.The position making buckle 600 is fixed, even if the position of also fixing with buckle lid 400 is fixed such that it is able to realize the surface that the raised line 420 on lid is positioned at the through hole 301 of reaction chamber accurately.
In order to make the liquid sample in reaction chamber 300 pretreated, buffer or other reagent can be added in reaction chamber 300, in an embodiment, by reagents loaded on carrier 410, this carrier 410 is positioned in reaction chamber 300 in advance, to facilitate operation.More specifically, having draw-in groove on lid 400, be fixed in draw-in groove by carrier 410 one end, after lid 400 covers reaction chamber, carrier 410 is positioned at reaction chamber 300.
In some embodiments, lid 400 is additionally provided with a pore 430, makes reaction chamber 300 be connected with ambient atmosphere.
Secondly, sample collection detecting device 800 provided by the invention, except including the test chamber 100 of detecting device, sample reaction chamber 300, also includes collection well 500.In one embodiment, test chamber 100 is integral type structure with reaction chamber 300 and collection well 500.That is, same, test chamber 100 is also formed by base plate 102 and upper plate 101 engaging, testing element 200 is positioned at the draw-in groove of base plate 102 to be fixed, and wherein testing element 200 is multiple, more specifically, testing element is test strips, and this test strips is provided with sample region of acceptance 201, reagent area and detection zone.Sample reaction chamber 300 is for being vertically positioned on upper plate 101, its position is in the surface of the sample region of acceptance 201 of test strips 200, reaction chamber 300 is as bottom 304 by one end (sample receiving terminal) of upper plate 101, adding the columnar structured of the hollow that sidewall 305 encloses, its opening is accent 303.The bottom (upper plate 101) of reaction chamber 300 is provided with through hole 301, as in figure 2 it is shown, this through hole 301 is positioned at the top of the sample region of acceptance 201 of testing element 200 (test strips).When liquid passes through to flow out to test chamber 100 through hole 301 from reaction chamber 300, the sample region of acceptance 201 of liquid first engaged test element.As shown in Figure 1, collection well 500 is the cavity of the one end open 520 enclosed by sidewall 530 and bottom 540 equally, this cavity 500 is positioned at one end of the upper plate 101 of test chamber 100, it is connected as a single entity with upper plate 101, meanwhile, collecting chamber 500 and reaction chamber 300 link together, concrete, the opening direction of collecting chamber and the opening direction of reaction chamber are mutually perpendicular to, namely collecting chamber 500 is laterally positioned on test chamber upper plate 101, namely collecting chamber sidewall 530 is connected with test chamber upper plate 101, namely the bottom of collecting chamber 500 is connected with the sidewall of reaction chamber 300, more specifically, the bottom of collecting chamber 500 is the sidewall of reaction chamber 300 simultaneously.In the bottom of collecting chamber 500, it is provided with through hole 510 and makes collecting chamber 500 connect with reaction chamber 300.In the present invention, cleverly collecting chamber 500 and reaction chamber 300 are fully integrated on the upper plate 101 of test chamber, and collecting chamber 500 and reaction chamber 300 also integrate, make whole sample collection detecting device 800 moulding light and handy, and enable sample to collect, pretreatment and detection integral type complete, convenient to operation.Concrete, in order to easy to operate, good looking appearance, collect/500 to be mutually perpendicular to reaction chamber 300, namely reaction chamber 300 is vertically positioned on test chamber upper plate 101, and collecting chamber 500 laterally crouches at test chamber upper plate 101, namely, the bottom 540 of collecting chamber links together with the sidewall 305 of reaction chamber, and the bottom 304 of reaction chamber is positioned at the top of sample application zone 201 of testing element 200.
This sample collection detecting device 800 also includes sample collection rod 700, collects rod 700 and mainly includes absorption piece 701 and handle 702, and the two is connected, sample is drawn by absorption piece 701, such as, put into absorption saliva sample in person under inspection oral cavity, be affectedly bashful collection rod by handle 702.
Collecting chamber 500 is combined with collection rod 700 and makes the liquid sample on absorption piece 701 be flowed out, specifically, absorption piece 701 is made to insert in collecting chamber 500, and move to collecting chamber 500 base portion, absorption piece 701 is made to contact bottom collecting chamber 500 and be compressed, it is arranged in the sample of absorption piece 701 to be extruded and pass out, flow in collecting chamber 500.Preferably, in order to make liquid will not be flowed out collecting chamber 500, there is the sealing ring 703 of annular in absorption piece 701 and handle 702 junction, as shown in Figures 2 and 3, this sealing ring 703 seals the sidewall of collecting chamber 500 collecting after rod 700 inserts collecting chamber 500, collecting chamber 500 is made to form a cavity sealed, avoid liquid outflow, simultaneously, along with the movement collecting rod 700, this sealing cavity volume is gradually reduced, and makes the pressure in annular seal space body increase, and is more beneficial for extruding absorption piece 701 and lets the liquid out.In some embodiments, after the absorption piece 701 collecting rod is all compressed in collecting chamber, collect rod 700 and be locked in collecting chamber 500, in specific embodiment, one end design that handle 702 is connected with absorption piece 701 has two symmetrical card articles 704, this card article 705 has elasticity, it is possible to by compression and collecting chamber 500.Having 2 symmetrical hole clippings 501 on the sidewall at the contiguous accent place of collecting chamber 500, after absorption piece 701 is all compressed, the salient point 705 on card article is placed exactly in hole clipping 501 and is fixed, and makes collection rod 700 be locked in collecting chamber 500.
First sample flows in collection well 500 after being extruded from absorption piece 701, is flowed in sample reaction chamber 300 again through through hole 510.On the sample collection detecting device 800 of the present invention, sample reaction chamber 300 is covered with lid 400, lid has pore 430, this pore 430 makes air inside and outside reaction chamber 300 identical, namely keeping the air pressure in reaction chamber 300 is atmospheric pressure, so, the liquid flowed out after being collected rod 700 in collecting chamber 500 and sealing and compress, because air pressure is more than the air pressure in reaction chamber, makes liquid sample pass through through hole 510 and flows into more smoothly.Having carrier 410 in the draw-in groove of lid 400, this carrier 410 is positioned at reaction chamber 300, and carrier 410 is loaded with the reagent of pretreatment sample, and after liquid sample flows into sample reaction chamber 300, contact carrier 410 reacts, it is achieved pretreatment.At lid 400 top, there is buckle 600, when the lid 400 with buckle covers after on reaction chamber 300, the raised line 420 being positioned at lid end is positioned at the surface of reaction chamber bottom through-hole 301, but the not through hole plug 310 on contact through hole, after taking off buckle 600, lower cover body 400, lid 400 top is made to contact with the accent of sample reaction chamber 300, in this process, raised line 420 contacts and pokes through hole plug 310, liquid sample pretreated in reaction chamber 300 is made to flow into test chamber 100 by the through hole plug 310 being punctured, contact with the sample region of acceptance 201 of test strips, complete detection.
Carry out the method for analyte in the collection of sample and detection sample below to be described with regard to the sample collection detecting device 800 of the present invention.
1. when initial position, as shown in Figure 3 and Figure 7, on the sample collection detecting device 800 of integral structure, annular buckle 600 connects covered body wall shrinkage pool 401 respectively by protruding 601 and protruding junction and reaction chamber groove 302 is fixed, lid 400 is positioned at primary importance, and now, the raised line 420 of lid end is positioned at reaction chamber 300, directly over through hole plug 310, do not contact with through hole plug 310；Now, collect rod 700 to separate with collecting chamber 500；
2. it is inserted into collecting absorption piece 701 one end collecting rod 700 having liquid sample towards horizontal collecting chamber 500, after absorption piece 701 fully enters collecting chamber 500, the sealing ring 703 being connected to absorption piece 701 end seals collecting chamber 500 accent place sidewall, makes collecting chamber 500 form the cavity sealed；
3. continuing to advance to collect rod 700, make absorption piece 701 be collected chamber 500 bottom squeeze compression, the liquid on absorption piece 701 flows out in collecting chamber 500；Meanwhile, flowed in reaction chamber 300 by the through hole 501 of collecting chamber 500 with reaction chamber 300；
4. after absorption piece 701 is all extruded, the salient point 705 of the card article 704 being positioned on handle is engaged to the bayonet socket 501 of collecting chamber sidewall, collects rod 700 and is locked in collecting chamber 500, as shown in Figure 4 and Figure 8；
5. making sample collection detecting device 800 stand 1 minute, now, the liquid sample being positioned at reaction chamber 300 contacts with the reagent on carrier 410 and reacts, and completes the pretreatment of sample；
6. take off the annular buckle 600 being fastened on lid 400, as shown in figs. 5 and 9；
7. press down on lid 400, in the process of pressing, the pore 430 of lid is closed；In the process, raised line 420 moves bottom reaction chamber 300 with lid 400, comes into contact with and puncture through hole plug 310；
8., when the accent place of the top contact of lid 400 to reaction chamber 300, stop pressing, now, lid 400 is positioned at the second position, and now, through hole plug 310 is punctured, liquid sample via through holes 301 in reaction chamber 300 enters the sample region of acceptance 201 of the test strips 200 in test chamber 100, detects.
Claims (18)
1. a sample testing apparatus, including test chamber, it is characterised in that described sample testing apparatus also includes sample reaction chamber, is provided with a passage between test chamber and sample reaction chamber；Described passage is closed when original state and is made test chamber and sample reaction chamber liquid be not attached to lead to；Described passage is opened when the state of detection and is made test chamber and sample reaction chamber liquid communication.
2. sample testing apparatus according to claim 1, it is characterised in that described sample reaction chamber is connected in test chamber, the bottom of described sample reaction chamber is connected to the sample receiving terminal of test chamber；Described passage is be positioned at the through hole being connected bottom sample reaction chamber with test chamber；During original state, through hole is sealed；During detection state, the through hole of sealing is unlocked.
3. sample testing apparatus according to claim 2, it is characterised in that described through hole is provided with the sealing-plug sealing through hole.
4. sample testing apparatus according to claim 1, it is characterised in that described sample testing apparatus also includes lid, described lid is used for covering and is positioned at sample reaction chamber；Lid end is connected to raised line.
5. sample testing apparatus according to claim 4, it is characterised in that after lid covers sample reaction chamber, raised line is positioned at directly over reaction chamber inner via hole.
6. sample testing apparatus according to claim 5, it is characterised in that sample testing apparatus also includes ring-type buckle, buckle is fixed on the sidewall of lid top；Buckle center is provided with projection, covered body wall has and holds protruding shrinkage pool, and after described buckle projection snap-on caps body sidewall shrinkage pool, lid and buckle interfix.
7. sample testing apparatus according to claim 6, it is characterised in that lid has primary importance and the second position on sample reaction chamber；During original state, when lid is positioned at primary importance, buckle is fastened between lid and sample reaction chamber and fixing lid, and lid top not contacting reaction cavity accent, the raised line on lid does not contact the through hole plug on sample reaction chamber bottom through-hole；During detection state, when lid is positioned at the second position, buckle is removed, lid directly covers after being pressed down on sample reaction chamber, the direct contacting reaction cavity accent in lid top, the raised line on lid contacts and pokes the through hole plug bottom sample reaction chamber, makes sample reaction chamber be connected with test chamber.
8. sample testing apparatus according to claim 4, it is characterised in that lid end is additionally provided with draw-in groove；In draw-in groove, engaging has chip carrier.
9. sample testing apparatus according to claim 2, it is characterised in that include one or more testing element in test chamber；The sample region of acceptance of described testing element is positioned at the lower section of sample reaction chamber bottom through-hole.
10. a sample collection detecting device, including test chamber, it is characterised in that also include sample reaction chamber, collection well；The bottom of described sample reaction chamber is connected to the sample receiving terminal of test chamber, and described sample reaction chamber is connected with collection well and connects；Being provided with a passage between test chamber and sample reaction chamber, described passage is be positioned at the through hole being connected bottom sample reaction chamber with test chamber；During original state, through hole is sealed；During detection state, the through hole of sealing is unlocked.
11. sample collection detecting device according to claim 10, it is characterised in that described collection well is positioned at one end of test chamber；The bottom of described collection well is connected with sample reaction chamber sidewall；There is bottom collecting chamber the through hole connected with sample chamber sidewall.
12. sample collection detecting device according to claim 11, it is characterised in that also include the lid covering sample reaction chamber；Lid end is connected to raised line.
13. sample collection detecting device according to claim 12, it is characterised in that also including ring-type buckle, buckle is fixed on covered body wall；Buckle center is provided with projection, covered body wall has and holds protruding shrinkage pool, and after described buckle projection snap-on caps body sidewall shrinkage pool, lid and buckle interfix.
14. sample collection detecting device according to claim 13, it is characterised in that lid has primary importance and the second position on sample reaction chamber；When lid is positioned at primary importance, buckle is fastened between lid and sample reaction chamber and fixing lid, and the raised line on lid does not contact the through hole plug of sample reaction chamber sealed bottom through hole；When lid is positioned at the second position, buckle is removed, and lid directly covers after being pressed down on sample reaction chamber, and the raised line on lid contacts and poke the through hole plug bottom sample reaction chamber, makes sample reaction chamber be connected with test chamber.
15. sample collection detecting device according to claim 11, it is characterised in that also including sample collection rod, described sample collection rod includes the absorption piece and the handle that are connected；And the seal member between absorption piece and handle.
16. sample collection detecting device according to claim 15, it is characterised in that collection well accent place is provided with two hole clippings of symmetry；The one end being connected with absorption piece on sample collection bat handle is provided with two card articles of symmetry.
17. the method collecting and detecting liquid sample, for analyzing the analyte in detection liquid sample, wherein, including a sample collection detecting device collecting and detecting analyte in liquid sample, this sample collection detecting device includes test chamber, sample reaction chamber, collection well；Described sample reaction chamber is connected with collection well and connects；Being provided with a passage between test chamber and sample reaction chamber, described passage is positioned at the bottom of the sample reaction chamber that the sample receiving terminal with test chamber is connected, and described passage is through hole；During original state, through hole is sealed；During detection state, the through hole of sealing is unlocked；Described sample collection detecting device also includes the lid covering sample reaction chamber and the raised line being connected to lid end, and be fixed the buckle on lid, and sample collection rod；Described specifically comprise the following steps that
(1) sample collection detecting device is when original state, and lid is fixed on the primary importance of sample reaction chamber by buckle, the through hole plug of the raised line not contacting reaction cavity bottom through-hole that lid end connects；
(2) insert collecting the sample collection rod having liquid sample in collection well, and make collecting chamber be collected rod sealing；
(3) continue to advance sample collection rod bottom collecting chamber, make the liquid on sample collection rod be flowed in collecting chamber and be flowed in sample reaction chamber by the through hole connected with the sidewall of sample reaction chamber bottom collection well；
(4) making the card article on collection rod be engaged in the hole clipping at collection well accent place makes collection rod be locked in collecting chamber；
(5) stand the regular hour, make the liquid sample in sample reaction chamber react with the material in reaction chamber；
(6) buckle being fastened on lid is taken off, press down on lid, lid is made to be covered to the accent of sample reaction chamber, namely lid is positioned at the second position of reaction chamber, now, sample collection detecting device is in detection state, and the end being positioned at the raised line of lid inner top contacts and poke the through hole sealed bottom sample reaction chamber；
(7) liquid sample enters test chamber, engaged test element by the through hole being punctured, and carries out the detection of analyte in liquid sample.
18. method according to claim 17, it is characterised in that the material in reaction chamber is the reagent being loaded on carrier；Carrier is fixed in reaction chamber by described one end by the draw-in groove engaging carrier on lid.
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| CN201610153236.8A CN105784443B (en) | 2016-03-16 | 2016-03-16 | A kind of sample testing apparatus and sample collection detection device and method |
| PCT/CN2016/083281 WO2017156869A1 (en) | 2016-03-16 | 2016-05-25 | Sample measurement device, and sample collection and measurement device and method |
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| CN201610153236.8A CN105784443B (en) | 2016-03-16 | 2016-03-16 | A kind of sample testing apparatus and sample collection detection device and method |
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| CN105784443B (en) | 2018-12-07 |
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