CN1057482A - The cell electricity merges, the electrotransfer device - Google Patents
The cell electricity merges, the electrotransfer device Download PDFInfo
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- CN1057482A CN1057482A CN 91104419 CN91104419A CN1057482A CN 1057482 A CN1057482 A CN 1057482A CN 91104419 CN91104419 CN 91104419 CN 91104419 A CN91104419 A CN 91104419A CN 1057482 A CN1057482 A CN 1057482A
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- cell electricity
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Abstract
The present invention relates to a kind of fusion of cell electricity, power conversion technology of being used for.This technology adopts and has the electrode of transmitter and have cell culture apparatus under the electromagnetic field effect of transmitter.But adopt the whole process that the present invention's pair cell electricity merges to carry out microscopic examination, micro tv videotapes, video, photomicrography.Also can carry out intelligent dynamic detection and control by microcomputer pair cell suspension properties (temperature, specific conductivity, pH value, solution ion concentration etc.).
Description
The present invention relates to a kind of device that is used for the fusion of cell electricity, electrotransfer, particularly relate to the device that is used in that the cell electricity merges, the electrotransfer process utilizes one chip microcomputer pair cell liquid characteristic to carry out intelligent dynamic detection and control; Also relate under electromagnetic field effect merging device that forward and backward cell cultivates and their using method.
Find out according to literature search, in European patent, United States Patent (USP), Japanese Patent, Deutsches Reichs-Patent, all do not find the electrode that in the fusion of cell electricity, electrotransfer technology, adopts belt sensor that this patent is applied for.
The pH value and the CO of disclosed pair cell liquid in European patent EP O333253
2Carry out detection of dynamic, just in cell cultivation process, carry out, be different from that this patent applies for merge at the cell electricity, pair cell liquid characteristic is carried out intelligent dynamic detection in the electrotransfer process; What also be different from present patent application is the intelligent dynamic detection that pair cell liquid characteristic and the electromagnetic intensity that applied are carried out in the cell cultivation process under the electromagnetic field effect.
Though in U.S. Pat .4578168 and US.4906576, used the modulation high-frequency electric field to make cell arrange bunchiness, but still carry out cytogamy with the video pulse electric field, the phase relationship of its high-frequency electric field and video pulse electric field is at random, and present patent application make cell arrange bunchiness with cytogamy the time electric field that put on the electrode be the relevant fully modulation high-frequency electric field of phase place.And the electrode that uses in the disclosed patented technology in U.S. Pat 4906576 and US4849089 is the parallel cylindrical disc electrode, and its electric field distribution is different fully with the Electrode Field distribution situation of present patent application.
The disclosed high frequency square wave of will modulating is used in cell and arranges bunchiness and cytogamy in the clear 63-49067 of Japanese Patent, and this signal is non-zero cross signal, and is different with the sine wave or the high frequency zero-crossing pulse electric field of the pulse amplitude modulation of present patent application.And its modulation amplitude of modulation high-frequency electric field of applying for of this patent, modulating pulse width, modulating pulse pitch time etc. all is subjected to intelligent control.
The clear 62-210978 of Japanese Patent and have the performance of high-frequency electric field amplitude, video pulse electric field amplitude being carried out intelligent control by Tianjin, SSH-1 type island cytogamy device that Japan produces according to this patented technology, but the width of its each video pulse that is applied in a test for fusion is all identical.And present patent application not only has the performance of high-frequency electric field amplitude, video pulse electric field amplitude being carried out intelligent control, and the plurality of video pulsed electrical field that in a test for fusion, is applied, its pulse width, pitch time can be identical, also can have nothing in common with each other, can also be train of impulses.
Though be cylindrical electrode at European patent EP 0225636, U.S. Pat 4804450 and the Japanese SSH-1 type island used electrode of Tianjin cytogamy device, but two electrodes all are fixed on the same substrate non-dismountable separation.When the spacing of electrode hour, clean, very inconvenience of sterilization.Built-up type cylindrical electrode in the present patent application and " outward " electrode are different fully with the electrode in the above-mentioned prior art.
The clear 62-265975 of Japanese Patent and Tianjin, Japanese SSH-1 type island cytogamy device have the equipment of (or forward and backward) controlled temperature before the fusion of cell electricity, electrotransfer.And present patent application is pair cell liquid temp and the Intelligent Measurement of other parameter and the equipment of control in its cell electricity fusion, electrotransfer process.
The clear 63-173573 of Japanese Patent disclosed two interelectrode insulants are in order to insulate between the spacing that guarantees two electrodes and electrode not in electric converged container.The electric insulation layer that " outward " electrode in the present patent application is had between two electrodes then is in order to guarantee that high-potential electrode does not contact with enchylema, can electrical isolation, and carry high frequency energy effectively again.
The disclosed polymeric membrane accompanying outside electrode of the clear 63-173574 of Japanese Patent is for the needed cell of penetrating screening selectively, does not all have electrical insulation characteristics between its two electrode and enchylema.Disclosedly among the Japanese Patent 63-49066 adhere to foraminate dielectric layer outside electrode, remove for the needed extracellular of penetrating screening selectively, also for aperture place electroline is concentrated, its electrode is directly to contact with enchylema.Though be parallel pole and the barrier film that has Mierocrystalline cellulose or synthon between electrode in the clear 63-84478 of Japanese Patent, its membranous effect is to build up cell.Though cell does not contact with electrode, electrode contacts with enchylema.Disclosed device all is different from " outward " electrode device in the present patent application in the above-mentioned prior art.The electric insulation layer of " outward " electrode of present patent application is for high-potential electrode does not contact and electrical isolation with enchylema, guarantees effective transmission of modulated high frequency signal energy again.
Disclosed electrode form is insulation layer " outward " electrode with the air among U.S. Pat 4663292 and Advanced Gene Transfer System BAEKON 2000 SERIES 1 that produced by U.S. BAEKON company according to this patented technology, the electric field that is applied is some groups of non-zero passage high-frequency electric fields, and each is organized, and phase place is at random between high-frequency electric field.Electrode shape in the above-mentioned prior art all is different from cylindrical " outward " electrode and parallel " outward " electrode in the present patent application, and the phase place of the employed zero passage modulation of present patent application high-frequency electric field is relevant fully.In addition, the electrode in the present patent application not only is convenient to clean, sterilization, and is also reusable.And BAEKON 2000 SERIES 1 instrument of being produced according to U.S. Pat 4663292 (domestic existing import), show in actual use between its ground-electrode and plastic containers and can infiltrate enchylema, be not easy to clean and sterilization, also can get rusty through repeatedly using ground-electrode.
Purpose of the present invention provides the fusion of a kind of cell electricity, electrotransfer device just for the deficiency that overcomes above-mentioned prior art.Adopt that this device and configuration one chip microcomputer and Circuits System are implemented in that the cell electricity merges, pair cell liquid characteristic is carried out Intelligent Measurement and control in the electrotransfer process, pair cell liquid characteristic and electromagnetic intensity are carried out Intelligent Measurement and control in cell cultivation process.
The present invention adopts the fusion of built-up type cell electricity, the electrode power transfer that has transmitter.This electrode constitutes by being installed on upper and lower two two concentric round metal cylindricality electrodes on glass respectively, and two electrodes are connected to contact conductor respectively.This electrode also can adopt " outward " electrode, and promptly ground-electrode contacts with enchylema, between high-potential electrode and enchylema electric insulation layer is arranged.The present invention has also adopted the electromagnetic field cell culture apparatus that has survey sensor.
Below in conjunction with accompanying drawing the present invention is elaborated:
Fig. 1 a shows the electrode structural chart of belt sensor
Fig. 1 b shows A-A ' sectional drawing of Fig. 1 a
Fig. 2 a shows cylindrical " outward " electrode structural chart
Fig. 2 b shows parallel " outward " electrode structural chart
Fig. 2 c shows the B-B ' sectional drawing of Fig. 2 b parallel " outward " electrode
Fig. 3 a, 3b are added in the voltage waveform view on " outward " electrode when showing work
Fig. 4 shows the cell culture apparatus structure iron under the electromagnetic field effect
The one chip microcomputer that Fig. 5 a demonstration Fig. 1 device is used and the skeleton diagram of Circuits System
The one chip microcomputer that Fig. 5 b demonstration Fig. 2 device is used and the skeleton diagram of Circuits System
The cell electricity that has transmitter merges, electrode power transfer is that cylindrical metal electrode 1,2 is installed respectively on two blocks of glass 3,4.The concentricity of electrode 1,2 and the sealing of entire electrode are guaranteed by annulus 7.Survey sensor group 8,9,10 is installed on electrode 2 and the glass 4.Be connected to contact conductor 5,6 on electrode 1 and the electrode 2.Constitute like this a sealing, combined electrode.Cylindrical " outward " electrode is that high-potential electrode 12 is installed on glass 14, and electrode 12 skins have insulation layer 11, and ground-electrode 13 is installed on the glass 21, on electrode 13 and the glass 21 sensor groups 18,19,20 is housed.Annulus 17 is used for guaranteeing the sealing of the concentricity and the electrode integral body of electrode 12,13.Be connected to contact conductor 15,16 on the electrode 12,13.
Parallel " outward " electrode is a parallel installation high-potential electrode 23 on glass 29, ground-electrode 24.The skin of electrode 23 has electric insulation layer 22.Insulating end plate 27,28 is equipped with at the two ends of electrode 23,24.One end of electrode 23,24 respectively is connected to contact conductor 25,26, and transmitter 30 is contained between two electrodes.
The cell culture apparatus that has under the electromagnetic field effect of transmitter is that light-permeable electrode 31, electromagnetic field electrode 32 and electromagnetic intensity survey sensor 33 are installed on glass 35.Can place by culturing cell 36 on the light-permeable electrode, and measure by sensor groups 34 pair cell liquid characteristics.
The advantages such as employing the present invention is beneficial to the aseptic test of cell, and is easy to use, has simultaneously cleaning, sterilization conveniently, and the cell suspending liquid capacity is big. Adopt electrode described in the present invention to carry out micro-observation, micro tv shooting, video recording, photomicrography to the overall process that the cell electricity merges, also can carry out intelligent dynamic detection and control to the characteristic (temperature, electrical conductivity, pH value, solution ion concentration etc.) of cell suspending liquid by microcomputer.
Claims (4)
1, a kind of device that is used for the fusion of cell electricity, electrotransfer is characterized in that this device adopts the fusion of cell electricity, the electrode power transfer that has transmitter.
2, device as claimed in claim 1 is characterized in that two concentric round metal cylindricality electrodes that the combined electrode of belt sensor is upper and lower by being separately fixed at, two on glass constitute, and two electrodes are connected to contact conductor respectively.
3, device as claimed in claim 1 is characterized in that adopting " outward " electrode, and promptly ground-electrode contacts with enchylema, between high-potential electrode and enchylema electric insulation layer is arranged.
4, a kind of cell electricity fusion, electrotransfer device, it is characterized in that adopting the electromagnetic field cell culture apparatus that has survey sensor, this device constitutes by light-permeable electrode, electromagnetic field electrode, electromagnetic intensity survey sensor and enchylema feature measurement transmitter are installed on the sheet glass, places by culturing cell on the light-permeable electrode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 91104419 CN1025920C (en) | 1991-07-04 | 1991-07-04 | Cell electrofusion electrotransfer device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 91104419 CN1025920C (en) | 1991-07-04 | 1991-07-04 | Cell electrofusion electrotransfer device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1057482A true CN1057482A (en) | 1992-01-01 |
| CN1025920C CN1025920C (en) | 1994-09-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 91104419 Expired - Fee Related CN1025920C (en) | 1991-07-04 | 1991-07-04 | Cell electrofusion electrotransfer device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1025920C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1058291C (en) * | 1993-03-26 | 2000-11-08 | 中国科学院等离子体物理研究所 | Cell modification technique with low energy ion beam |
| CN100355874C (en) * | 2006-03-09 | 2007-12-19 | 重庆大学 | Chip for realizing cell fusion |
-
1991
- 1991-07-04 CN CN 91104419 patent/CN1025920C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1058291C (en) * | 1993-03-26 | 2000-11-08 | 中国科学院等离子体物理研究所 | Cell modification technique with low energy ion beam |
| CN100355874C (en) * | 2006-03-09 | 2007-12-19 | 重庆大学 | Chip for realizing cell fusion |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1025920C (en) | 1994-09-14 |
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