CN105702146B - Bionical dynamic mouse stomach-duodenum digestive system simulator and analogue experiment method - Google Patents
Bionical dynamic mouse stomach-duodenum digestive system simulator and analogue experiment method Download PDFInfo
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- CN105702146B CN105702146B CN201511015935.8A CN201511015935A CN105702146B CN 105702146 B CN105702146 B CN 105702146B CN 201511015935 A CN201511015935 A CN 201511015935A CN 105702146 B CN105702146 B CN 105702146B
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Abstract
The present invention relates to bionical dynamic mouse gastroduodenal digestive system simulator and analogue experiment methods, bionical dynamic mouse gastroduodenal digestive system simulator, including peptic digest system, duodenum digestive system, stream plus emptier and incubation chamber, stream plus emptier convey gastric juice towards peptic digest system, bile and pancreatic juice are conveyed towards duodenum digestive system, peptic digest system and duodenum digestive system are interconnected.Bionical dynamic mouse gastroduodenal digestive system analogue experiment method, includes the following steps：（1）Adjust the room temperature in incubation chamber；（2）Filling gastric juice, pancreatic juice and bile；（3）Prepare foodstuff samples；（4）Gastric juice is injected to bionic-mouse stomach model；（5）Foodstuff samples are injected to bionic-mouse stomach model；（6）Simulate digestion process；（7）Time segment records digestion process.The present invention has more actually analog result, extremely similar to the digestion process of mouse living, has good application value.
Description
Technical field
The present invention relates to bionical dynamic mouse stomach-duodenum digestive system simulator and analogue experiment method, the digestion
System simulator can be used for the mixing in true mouse stomach of the food and drug of bionical a variety of different types and structure in vitro,
Grinding, digestion and evacuation procedure.
Background technology
The new functional food of any type and drug are putting goods on the market by consumer and patient's trust and are receiving it at present
Before, it is necessary to carry out the experiment and evaluation of stringent nutritive value, toxicity, pharmacology etc..And to assess its nutriment and
Release rate and utilization rate of the effective active composition in gastrointestinal tract, first it is to be understood that their digestion and evacuation procedure in stomach,
Recognize its nutrient delivery process in vivo, so as to improve the new functional food of the processing method or structure of food and drug,
Effectively improve nutritional ingredient digest and assimilate and the utilization rate of drug.In addition, as food-safety problem becomes increasingly conspicuous, this is to having
Effect, quickly, easily the residual technology of detection food additives and poisonous and harmful element in vivo proposes new challenge.
Study of functional food and drug digestion, emptying and effective active composition process in the gastrointestinal tract and toxic
The residual of harmful substance in vivo, conventional method mainly resort to living animal and human volunteer's clinical test.Due to work
The selection of body or clinical trial object is restricted, and because bion physiology（Including gender, age, medical history etc.）Difference
The influence of different, architectural difference and environmental factor to organism state, cause live body and clinical test it is time-consuming and laborious, it is of high cost,
Poor reproducibility, and also suffer from the limitation in terms of ethics.External Bionic digestion road, be digestive system to human body or animal and
Its digestive environments, computational fluid dynamics behavior in the digestive tract carry out the device of human simulation, not only without the limitation in terms of ethics, and
And live body and clinical test can be replaced completely or partially, or come test situation and optimization in predictor as " preceding test " and live
Body experimental condition, to achieve the purpose that reduce cost, save time, raising repeatability and accuracy.
In recent years, researchers develop a variety of external digestion system models, for studying the object during food digestion
Physicochemical variation, between food interaction, beneficial bacteria of intestinal tract survival rate, the development and application of functional food and drug and
Residual and metabolic process of the poisonous and harmful substance in gastrointestinal tract.Current external digestion system model can divide according to elastic size
For rigid system, semi-rigid system and flexiblesystem.The outer stomach of rigid body or gastrointestinal model are using simple receptacles such as beakers by stirring
Instrument or shaking table concussion equipment are mixed to drive gastric content to mix, simplicity is cheap, drives（Rotating speed）Controllably, but lack to stomach or intestines
Physiology and appearance, stomach or the enteral physicochemical environment in road, stomach wall or effective simulation of intestinal wall movement and fluid dynamics behavior.Thus,
Recent study persons are more likely to research with stomach and intestinal physiology morphological feature, stomach wall and intestinal wall is movable, gastric juice, intestinal juice
With chyme continuously secrete and empty etc. it is increasingly complex, closer to bionical peptic digest system outside the dynamic body of true stomach and enteron aisle.Mesh
The preceding more extensive external digestion system of application includes mainly the DGM developed by Leeds, England Hai De Food Research Inst.
The TIM (TNO Intestinal Model) of (Dynamic Gastric Model), Holland's TNO nutrition and Food Research Inst.
With the HGS (Human Gastric Simulator) of Univ California-Davis USA.These three classical external digestions
System respectively has quality, physical and chemical factor that can effectively in simulated gastrointestinal tract, and reconstructing portion food or drug are in gastrointestinal tract
In digestion, emptying and effective active composition release behavior.But they do not have the form and physiology of true stomach or enteron aisle
The details of structure（Size, stomach inner wall fold etc.）, distribution and digestion of the food in true stomach, purge sequence can not be reappeared, and
And effective simulation of the shortage to stomach wall or intestinal wall peristaltic contraction.In addition, these systems construction is complicated, cost is higher, dismounting
It is inconvenient with cleaning；The related physiologic parameters of human gastrointestinal tract are obtained since ethics limitation can not facilitate, cause there cannot be effect
Demonstrate,prove the validity and science of above system.
For this purpose, structure a kind of form and physiology details with true stomach and enteron aisle, can effectively simulate stomach wall and intestinal wall is compacted
It moves contraction and low cost, dynamic bionic easy to operate stomach-duodenum digestive system will be with a wide range of applications.
Invention content
Above-mentioned in order to solve the problems, such as, the invention discloses bionical dynamic mouse stomach-duodenum digestive system simulations
Device and analogue experiment method, specific technical solution are as follows：
Bionical dynamic mouse stomach-duodenum digestive system simulator, the simulator is for simulating foodstuff samples in mouse
Stomach back warp cross the digestion after Gastric juice digestion using pancreatic juice and bile in duodenum, for study digestion process neutralize most
Whole chyme variation, the simulator include peptic digest system, duodenum digestive system, stream plus-emptier and incubation chamber,
The peptic digest system, duodenum digestive system and stream plus-emptier are respectively positioned in incubation chamber, stream plus-emptier court
Gastric juice is conveyed to peptic digest system, bile and pancreatic juice, peptic digest system and duodenum are conveyed towards duodenum digestive system
Digestive system is interconnected,
The peptic digest system includes bionic-mouse stomach model and electric compressor-rolling extrusion instrument, the bionic-mouse stomach mould
Foodstuff samples are housed, the electric compressor-rolling extrusion instrument is used for rolling extrusion bionic-mouse stomach model, bionic-mouse stomach mould in type
Type generates simulation stomach contraction-rolling digestion action；
The duodenum digestive system includes bionical duodenum model pipe and electric peristaltic squeezes instrument, described bionical
Foodstuff samples in mouse stomach model are discharged into after the digestion of bionic-mouse stomach model in bionical duodenum model pipe, described electronic
It wriggles and squeezes instrument for the bionical duodenum model pipe of extruding of wriggling, bionical duodenum model pipe generates simulation duodenum
The action of wriggling digestion；
The stream plus-emptier include single channel syringe pump A, single channel syringe pump B and single channel syringe pump C, described
Single channel syringe pump A is connected to bionic-mouse stomach model by gastric juice pipe, and single channel syringe pump A is towards conveying stomach in bionic-mouse stomach model
Liquid, the single channel syringe pump B are connected to bionical duodenum model pipe by pancreatic juice pipe, and single channel syringe pump C passes through bile pipe
It is connected to bionical duodenum model pipe, single channel syringe pump B is towards conveying pancreatic juice, single channel note in bionical duodenum model pipe
Pump C is penetrated towards conveying bile in bionical duodenum model pipe；
The free end of the bionical duodenum model pipe is provided with chyme receiving flask, and the chyme receiving flask is for collecting
The chyme being discharged from bionical duodenum model pipe.
The bionic-mouse stomach model is to make to be molded by overmolded using the mouse stomach of true full abdomen size, is used
" Dragon Skin " addition-type silicon rubber is made, size, shape and the internal structure of the bionic-mouse stomach model with it is true
Real rat stomach is consistent, using boundary ridge as boundary, is divided into glandular stomach and glandular stomach, glandular stomach inner wall smooth, glandular stomach inner wall is full of fold, and glandular stomach connects
Lead to oesophagus, silica gel valve pipe is installed in oesophagus, glandular stomach is connected to pyloric canal and gastric secretion pipe, and the gastric secretion pipe connection is single
Channel injection pumps A, and the pyloric canal is connected to bionical duodenum model pipe, and foodstuff samples enter bionic-mouse stomach model from oesophagus,
Food becomes chyme after the digestion of bionic-mouse stomach model and is expelled to bionical duodenum model pipe from pyloric canal.
The electric compressor-rolling extrusion instrument includes stepper motor, holder, eccentric wheel, frequency controller, gusset, skin
Band, belt pulley, bevel gear, pressing plate, fixed plate, support plate and pedestal, the holder are fixed on the base, and are arranged on the holder
There are two fixed plate, support plate is fixed between described two fixed plates, the bionic-mouse stomach model is installed on the supporting plate, often
There are one stepper motors, an end of stepper motor to pass through bevel gear connecting eccentric wheel, bias rotation for installation in a fixed plate
Compression vertical up and down is carried out to glandular stomach with dynamic pressure plate during dynamic, the fixed plate is additionally provided with three of distribution triangular in shape
Belt pulley, belt is cased on the belt pulley, and one of belt pulley has stepper motor driving, stepper motor to drive belt in turn
Eccentric wheel is driven to carry out from antrum toward pylorus direction rolling extrusion glandular stomach, the frequency controller is connect with stepper motor, frequency
Rate controller is compressed adjusting bionic-mouse stomach model by controlling the rotating speed of stepper motor and rolling extrusion frequency and amplitude.
The length and internal diameter and the duodenal length of true mouse and internal diameter size phase of the bionical duodenum model pipe
Deng pyloric canal is connected with bionical duodenum model pipe by the four-way pipe of " K " word shape, the four-way pipe of " K " word shape
One end of the part " I " be connected with pyloric canal, the other end is connected with bionical duodenum model pipe, the four-way pipe of " K " word shape
One end of the part " ＜ " be connected with single channel syringe pump B by pancreatic juice pipe, the other end passes through bile pipe and single channel syringe pump C
It is connected.
The external electric wriggle squeeze instrument include stepper motor, eccentric wheel, frequency controller, bevel gear, fast pulley,
Support plate, fixed plate, belt, belt pulley and pedestal, the holder are fixed on the base, and fixed plate, institute are fixed on the holder
It states and several fast pulleys is set in fixed plate, the bionical duodenum model pipe is curved through each fast pulley successively, described
The side of fixed plate is provided with two stepper motors, and every stepper motor is described inclined by bevel gear connecting eccentric wheel
Heart wheel carries out subsection interleaving formula wriggling to bionical duodenum model pipe and squeezes, and the frequency controller connects stepper motor, frequency
Rate controller squeezes frequency to adjust eccentric wheel by controlling the rotating speed of stepper motor to the wriggling of bionical duodenum model pipe.
The chyme upstream end of the bionical duodenum model pipe is provided with peristaltic pump.
It is provided in the incubation chamber for the heating lamp of air in heating heat-preserving box and for being shone in incubation chamber
Bright headlamp is additionally provided with the digital display temperature controller for monitoring the real time temperature inside incubation chamber, the guarantor in the incubation chamber
The side of warm box is provided with sliding door.
Using the simulated experiment side of any of the above-described bionical dynamic mouse stomach-duodenum digestive system simulator
Method includes the following steps：
（1）Adjust the room temperature in incubation chamber：The heating lamp and digital display temperature controller in incubation chamber are opened, waits for the temperature in incubation chamber
When degree maintains 37 DEG C, heating lamp is closed；
（2）Filling gastric juice, pancreatic juice and bile：A gastric juice pipe is taken, wherein filling full gastric juice, then by the one of gastric juice pipe
End is connected with single channel syringe pump A, and the other end is connected with pyloric canal, a pancreatic juice pipe and a bile pipe is separately taken, in pancreatic juice pipe
In filling full pancreatic juice, one end and the single channel syringe pump B of pancreatic juice pipe connect, the filling full bile in bile pipe, the one of bile pipe
End is connected with single channel syringe pump C, and the other end of pancreatic juice pipe is connected with the four-way pipe of the other end " K " word shape of bile pipe；
（3）Prepare foodstuff samples：It takes food and is placed in the container equipped with deionized water, after being sufficiently stirred, mouse saliva is added,
Then magnetic agitation, to simulate food in the intraoral digestion process of mouse；
（4）Gastric juice is injected to bionic-mouse stomach model：Simulate the gastric juice is injected to bionic-mouse stomach model with syringe, it is big to simulate
Gastric juice remains when mouse stomach fasting state；
（5）Foodstuff samples are injected to bionic-mouse stomach model：By step（3）In the foodstuff samples that are prepared it is primary from esophagus
Property be injected into bionic-mouse stomach model, the outlet of silica gel valve pipe and oesophagus is connected, prevents food in stomach from overflowing, and keeps stomach
Interior voltage stabilizing；
（6）Simulate the digestion process of bionic-mouse stomach model and bionical duodenum model pipe：Initiation culture controller, it is electronic
Compression-rolling extrusion instrument starts vertically to compress the glandular stomach of bionical rat stomach model, while to glandular stomach from antrum
Toward the rolling extrusion in pylorus direction, gastric juice is pushed in bionic-mouse stomach model by single channel syringe pump A, is noted by single channel
It penetrates pump B pancreatic juice is pushed in bionical duodenum model pipe, bile, which is pushed to bionical 12, by single channel syringe pump C refers to
In intestines model pipe, pancreatic juice and bile and the chyme being discharged from bionic-mouse stomach model are transported to bionical duodenum by peristaltic pump
In model pipe, electric peristaltic squeezes instrument and carries out segmentation to bionical duodenum model pipe and wriggle to squeeze, finally by chyme from imitative
It is discharged in raw duodenum model pipe, is collected bottle collection；
（7）Time segment records digestion process：In step（6）In the digestion process, when digestion process is divided into several
Between section, stop a frequency controller, peristaltic pump, single channel syringe pump A, single channel syringe pump B and single-pass in each period
Road syringe pump C takes out bionic-mouse stomach model and bionical duodenum model pipe, collects bionic-mouse stomach model and bionical 12 respectively
Digest in duodenum 12 model pipe measures and records respectively its pH, maltose concentration, gastric emptying rate, storage modulus, loss mould
Chyme, is then put back to bionic-mouse stomach model and bionical ten by amount, dynamic viscosity and apparent viscosity, and observation microstructure again
In two duodenum 12 model pipes, frequency controller, compacted will be opened in bionic-mouse stomach model and bionical duodenum model pipe retrieval device
Dynamic pump, single channel syringe pump A, single channel syringe pump B and single channel syringe pump C, continue to test, until chyme is all from bionical ten
It is discharged in two duodenum 12 model pipes, and measures and record pH, maltose concentration, gastric emptying rate, the energy storage mould of final discharge chyme
Amount, loss modulus, dynamic viscosity and apparent viscosity, and observation microstructure；
（8）Clean bionic-mouse stomach model and bionical duodenum model pipe：After experiment, cleaned in time with deionized water
Bionic-mouse stomach model and bionical duodenum model pipe.
The step（7）Middle microstructure is observed by laser confocal microscope CSLM and scanning electron microscope sem.
The present invention operation principle be：
The present invention is made the paraffin mold of bionic-mouse stomach model using the specification of mouse stomach under true full abdomen state, made
Bionic-mouse stomach model out can more true simulation mouse stomach.The present invention, using electric compressor-rolling extrusion instrument to bionical
The glandular stomach that carries out of mouse stomach model carries out compression vertical up and down, carries out glandular stomach from antrum toward pylorus direction rolling extrusion, can be more
Really digestion acts true simulation mouse stomach.
The specification of the present invention, bionical duodenum model pipe are consistent with the duodenum specification of true mouse, and it is real to improve simulation
The authenticity tested.The present invention squeezes instrument using external electric wriggling and is squeezed into line crawl bionical duodenum model pipe, mould
Quasi- digestion change procedure of the chyme in mouse duodenum.
The present invention, by the foodstuff samples after mouse salivary digestion successively bionic-mouse stomach model and bionical duodenum model
Pipe, can simulate realistically foodstuff samples continuous digestion process in the stomach and duodenum of mouse.
The beneficial effects of the invention are as follows：
1. due to including mouse peptic digest system and duodenum digestive system two large divisions, thus the present invention can compare
Complete digestion process of the comprehensive simulation food in stomach and duodenum dynamic and can accurately reappear in true rat stomach
Digestive environments；
2. bionic-mouse stomach model is using high resiliency, the addition-type silicon rubber raw material of high stability, the high grade of transparency, by true
Mouse stomach 1:1 overmolded makes, and has the form of true rat stomach, the details of size and physiological structure（Inner wall fold etc.）；
3. bionical duodenum model pipe is using silica gel hose making, length and internal diameter size and true mouse duodenum
It is similar；
4. electric compressor-rolling extrusion instrument and external electric are wriggled, extruding instrument highly can approximately reappear true
Mouse stomach and duodenal peristaltic contraction frequency and amplitude, and the peristaltic contraction shape of true mouse stomach stomach wall and intestinal wall can be simulated
Formula；
5. using single channel syringe pump A, single channel syringe pump B, single channel syringe pump C and peristaltic pump, can accurately simulate
The rate of evacuation of gastric juice, the secreting rate of pancreatic juice and bile and chyme；
6. it is the small, low cost of the present invention, easy to operate, easy to clean, it can be used in the digestion to food and drug
Carry out in vitro study.
Description of the drawings
Fig. 1 is the bionic-mouse stomach model structural schematic diagram of the present invention,
Fig. 2 is the peptic digest system structure diagram of the present invention,
Fig. 3 is the E direction views of Fig. 2,
Fig. 4 is the F direction views of Fig. 3,
Fig. 5 is the duodenum digestive system structural schematic diagram of the present invention,
Fig. 6 is the G direction views of Fig. 5,
Fig. 7 is the H direction views of Fig. 5,
Fig. 8 is the structural schematic diagram of the present invention,
Reference numerals list：1-glandular stomach, 2-glandular stomaches, 3-pyloric canals, 4-oesophaguses, 5-gastric juice pipes, 6-four-way pipes,
7-pancreatic juice pipes, 8-bile pipes, 9-bionical duodenum model pipes, 10-pedestals, 11-holders, 12-fixed plates, 13-partially
Heart wheel, 14-bionic-mouse stomach models, 15-bevel gears, 16-belt pulleys, 17-belts, 18-stepper motors, 19-peristaltic pumps,
20-single channel syringe pump A, 21-single channel syringe pump B, 22-single channel syringe pump C, 23-frequency controllers, 24-chymes
Receiving flask, M-electric compressor-rolling extrusion instrument, N-electric peristaltic squeeze instrument.
Specific implementation mode
With reference to the accompanying drawings and detailed description, the present invention is furture elucidated.It should be understood that following specific implementation modes are only
For illustrating the present invention rather than limiting the scope of the invention.
Fig. 8 is the structural schematic diagram of the present invention, and reference numeral is in figure：19-peristaltic pumps, 20-single channel syringe pump A,
21-single channel syringe pump B, 22-single channel syringe pump C, 23-frequency controllers, 24-chyme receiving flasks, the electronic pressures of M-
Contracting-rolling extrusion instrument, N-electric peristaltic squeeze instrument.In conjunction with attached drawing as it can be seen that this bionical dynamic mouse stomach-duodenum digestion
System simulator, the simulator exist after the stomach back warp of mouse crosses Gastric juice digestion using pancreatic juice and bile for simulating food
Digestion in duodenum, for studying in digestion process and the variation of final chyme, the simulator include peptic digest system,
Duodenum digestive system, stream plus-emptier and incubation chamber, the peptic digest system, duodenum digestive system and stream plus-
Emptier is respectively positioned in incubation chamber, and stream plus-emptier convey gastric juice towards peptic digest system, towards duodenum Digestive
System conveying bile and pancreatic juice, peptic digest system and duodenum digestive system are interconnected, and the present invention, food disappears by stomach successively
Change system and duodenum digestive system imitate the digestion of this two big digestive system.
The peptic digest system includes bionic-mouse stomach model and electric compressor-rolling extrusion instrument, the bionic-mouse stomach mould
Food is housed, the electric compressor-rolling extrusion instrument is used for rolling extrusion bionic-mouse stomach model, the production of bionic-mouse stomach model in type
Raw simulation stomach contraction-rolling digestion action；Electric compressor-rolling extrusion instrument rolling extrusion bionic-mouse stomach model, simulation are bionical
Mouse stomach model is from line crawl digestion process.
The duodenum digestive system includes bionical duodenum model pipe and electric peristaltic squeezes instrument, described bionical
Food in mouse stomach model is discharged into after the digestion of bionic-mouse stomach model in bionical duodenum model pipe, the electric peristaltic
It squeezes instrument and squeezes bionical duodenum model pipe for wriggling, bionical duodenum model pipe generates simulation duodenum and wriggles
The action of digestion；Electric peristaltic squeezes instrument and squeezes bionical duodenum model pipe, simulates bionical duodenum model pipe
Voluntarily digestion process.
The stream plus-emptier include single channel syringe pump A, single channel syringe pump B and single channel syringe pump C, described
Single channel syringe pump A is connected to bionic-mouse stomach model by gastric juice pipe, and single channel syringe pump A is towards conveying stomach in bionic-mouse stomach model
Liquid, the single channel syringe pump B are connected to bionical duodenum model pipe by pancreatic juice pipe, and single channel syringe pump C passes through bile pipe
It is connected to bionical duodenum model pipe, single channel syringe pump B is towards conveying pancreatic juice, single channel note in bionical duodenum model pipe
Pump C is penetrated towards conveying bile in bionical duodenum model pipe；Simulation is more true to be digested in order to meet for gastric juice, pancreatic juice and bile
Process.
Single channel syringe pump A, single channel syringe pump B and single channel syringe pump C described in stream plus-emptier is by Baoding
Lange constant flow pump Co., Ltd produces, and model TJ-3A/W0109-1B can install the multiple standards syringe of mL specifications, line
Velocity interval is 7.94 μm of .min-1 - 79.4 mm.min-1。
The free end of the bionical duodenum model pipe is provided with chyme receiving flask, and the chyme receiving flask is for collecting
The chyme being discharged from bionical duodenum model pipe.Receiving flask collects chyme, not only prevents chyme from polluting experimental bench, also allows for
Further study chyme.
Fig. 1 is the bionic-mouse stomach model structural schematic diagram of the present invention, and reference numeral is：1-glandular stomach, 2-glandular stomaches, 3-is deep and remote
Men Guan, 4-oesophaguses, 5-gastric juice pipes, 6-four-way pipes, 7-pancreatic juice pipes, 8-bile pipes, 9-bionical duodenum model pipes.Knot
The attached drawing is closed as it can be seen that the bionic-mouse stomach model is that the mouse stomach of true full abdomen size is utilized to make molding, use by overmolded
" Dragon Skin " addition-type silicon rubber is made, size, shape and the internal structure of the bionic-mouse stomach model with it is true
Real rat stomach is consistent, using boundary ridge as boundary, is divided into glandular stomach and glandular stomach, glandular stomach inner wall smooth, glandular stomach inner wall is full of fold, and glandular stomach connects
Logical oesophagus, length of esophagus are 5 cm, silica gel valve pipe are equipped in oesophagus, glandular stomach is connected to pyloric canal and gastric secretion pipe, described
Gastric secretion pipe is connected to single channel syringe pump A, and the pyloric canal is connected to bionical duodenum model pipe, and food enters imitative from oesophagus
Raw mouse stomach model is expelled to bionical duodenum model pipe after bionic-mouse stomach model is digested to chyme from pyloric canal.
" Dragon Skin " high-performance A, B two-component addition-type silicon rubber, the bionical rat stomach made using the material
Silicone rubber model is translucent, do not dissolve in, with acid-base reaction, inviscid, tensile strength is not up to 475 psi, loses outer masterpiece
Restored as former state with that can spring back rapidly.
The length × width × height of bionic-mouse stomach model is respectively：40 (±3)×30（±2）×21（±2）Mm, maximum water storage
Volume is 9.0 ± 0.8 mL.
The wall of stomach thickness of glandular stomach and glandular stomach is respectively 0.65 ± 0.15 mm and 1.22 ± 0.25 mm.
The internal diameter and outer diameter of oesophagus are 3.0 ± 0.2 mm and 5.0 ± 0.2 mm respectively.
The internal diameter and outer diameter of silica gel valve pipe are 4.8 ± 0.2 mm and 7.0 ± 0.2 mm respectively, inside have two panels valve with
" V " type sticks at valve inside pipe wall, contacts with each other at two panels valve commissure but adhesion.
Fig. 2 is the peptic digest system structure diagram of the present invention, and Fig. 3 is the E direction views of Fig. 2, Fig. 4 be Fig. 3 F to regarding
Scheme, reference numeral is in figure：10-pedestals, 11-holders, 12-fixed plates, 13-eccentric wheels, 14-bionic-mouse stomach models,
15-bevel gears, 16-belt pulleys, 17-belts, 18-stepper motors.In conjunction with the three width attached drawing as it can be seen that the electric compressor-
Rolling extrusion instrument include stepper motor, holder, eccentric wheel, frequency controller, gusset, belt, belt pulley, bevel gear, pressing plate,
Fixed plate, support plate and pedestal, the holder are fixed on the base, and there are two fixed plates for setting on the holder, described two
Support plate is fixed between fixed plate, the bionic-mouse stomach model is installed on the supporting plate, in each fixed plate there are one installations
Stepper motor, an end of stepper motor is by bevel gear connecting eccentric wheel, and band dynamic pressure plate is to preceding in eccentric wheel rotation process
Stomach carries out vertical compression up and down, and the fixed plate is additionally provided with three belt pulleys of distribution triangular in shape, on the belt pulley
Be cased with belt, one of belt pulley has a stepper motor driving, stepper motor drive belt so that drive eccentric wheel to glandular stomach into
From antrum toward pylorus direction rolling extrusion, the frequency controller is connect row with stepper motor, and frequency controller is walked by controlling
The rotating speed of stepper motor is compressed adjusting bionic-mouse stomach model and rolling extrusion frequency and amplitude.
Gusset, pressing plate, support plate and the pedestal is all high-quality aluminium plate matter, and antirust treatment, fixed plate are passed through in surface
For acrylic material, thickness is 1.0 ± 0.2cm.
The rotating speed adjustable extent of the stepper motor rotating speed is 0-10 rpm.
The compression amplitude adjustable extent is 0-10 mm, rolling extrusion adjustable amplitude ranging from 0-5 mm.
The length and internal diameter and the duodenal length of true mouse and internal diameter size phase of the bionical duodenum model pipe
Deng pyloric canal is connected with bionical duodenum model pipe by the four-way pipe of " K " word shape, the four-way pipe of " K " word shape
One end of the part " I " be connected with pyloric canal, the other end is connected with bionical duodenum model pipe, the four-way pipe of " K " word shape
One end of the part " ＜ " be connected with single channel syringe pump B by pancreatic juice pipe, the other end passes through bile pipe and single channel syringe pump C
It is connected.
The internal diameter and outer diameter of the external bionical duodenum silica gel hose are respectively 3.0 ± 0.1 mm and 6.0 ± 0.2
mm。
The internal diameter and outer diameter of " I " part silicone gel pipe of K " the type four-way pipes be respectively 4.8 ± 0.2 mm and 7.0 ±
0.2 mm；The internal diameter and outer diameter of the part " ＜ " silicon rubber tubule are 1.0 ± 0.1 mm and 2.0 ± 0.1 mm respectively；
The internal diameter and outer diameter of pancreatic juice pipe and bile pipe are respectively 3.0 ± 0.2 mm and 5.0 ± 0.2 mm.
Fig. 5 is the duodenum digestive system structural schematic diagram of the present invention, and Fig. 6 is the G direction views of Fig. 5, and Fig. 7 is the H of Fig. 5
Direction view, in conjunction with the three width attached drawing, as it can be seen that the external electric is wriggled, extruding instrument includes stepper motor, eccentric wheel, frequency control
Device, bevel gear, fast pulley, support plate, fixed plate, belt, belt pulley and pedestal processed, the holder is fixed on the base, described
Fixed plate is fixed on holder, several fast pulleys are set in the fixed plate, and the bionical duodenum model pipe is bent successively
By each fast pulley, the side of the fixed plate is provided with two stepper motors, and every stepper motor is by boring tooth
Connecting eccentric wheel is taken turns, the eccentric wheel carries out subsection interleaving formula wriggling to bionical duodenum model pipe and squeezes, the frequency control
Device processed connects stepper motor, and frequency controller adjusts eccentric wheel to bionical duodenum mould by controlling the rotating speed of stepper motor
The wriggling of type pipe squeezes frequency.
The support plate and pedestal is all high-quality aluminium plate matter, and antirust treatment is passed through on surface, and fixed plate is acrylic material
Matter, thickness are 1.0 ± 0.2 cm.
The rotating speed adjustable extent of the stepper motor rotating speed is 0-20 rpm.
The eccentric wheel and fast pulley parallel arranged, four fast pulleys are vertically arranged, and four eccentric wheels are solid respectively two-by-two
It is scheduled on its left and right sides, the silica gel hose in duodenum model is staggeredly clipped between eccentric wheel and fast pulley.
The chyme upstream end of the bionical duodenum model pipe is provided with peristaltic pump.Peristaltic pump model 104K/BT
（stepper motor）, it is produced by Chongqing Jie Heng peristaltic pumps Co., Ltd, two kinds of wall thickness hoses of 1mm and 1.6 mm can be assembled,
1~4.8 mm of internal diameter, flow-control scope are 0-140 mL.min-1.
It is provided in the incubation chamber for the heating lamp of air in heating heat-preserving box and for being shone in incubation chamber
Bright headlamp is additionally provided with the digital display temperature controller for monitoring the real time temperature inside incubation chamber, the guarantor in the incubation chamber
The side of warm box is provided with sliding door.The shape of incubation chamber be cuboid, select PC plate be made, length × width × height be 85 ×
55×50 cm.The power of heating lamp is 100 W, from room temperature（25oC）It is heated to 37oC needs 15min.Sliding door is by sub- gram
Power plate, which is cut, to be made, long and wide respectively 42cm and 50 cm.
Using the simulated experiment side of any of the above-described bionical dynamic mouse stomach-duodenum digestive system simulator
Method includes the following steps：
（1）Adjust the room temperature in incubation chamber：The heating lamp and digital display temperature controller in incubation chamber are opened, waits for the temperature in incubation chamber
When degree maintains 37 DEG C, heating lamp is closed；
（2）Filling gastric juice, pancreatic juice and bile：A gastric juice pipe is taken, wherein filling full gastric juice, then by the one of gastric juice pipe
End is connected with single channel syringe pump A, and the other end is connected with pyloric canal, a pancreatic juice pipe and a bile pipe is separately taken, in pancreatic juice pipe
In filling full pancreatic juice, one end and the single channel syringe pump B of pancreatic juice pipe connect, the filling full bile in bile pipe, the one of bile pipe
End is connected with single channel syringe pump C, and the other end of pancreatic juice pipe is connected with the four-way pipe of the other end " K " word shape of bile pipe；
（3）Prepare foodstuff samples：It takes food and is placed in the container equipped with deionized water, after being sufficiently stirred, mouse saliva is added,
Then magnetic agitation, to simulate food in the intraoral digestion process of mouse；
（4）Gastric juice is injected to bionic-mouse stomach model：Simulate the gastric juice is injected to bionic-mouse stomach model with syringe, it is big to simulate
Gastric juice remains when mouse stomach fasting state；
（5）Foodstuff samples are injected to bionic-mouse stomach model：By step（3）In the foodstuff samples that are prepared it is primary from esophagus
Property be injected into bionic-mouse stomach model, the outlet of silica gel valve pipe and oesophagus is connected, prevents food in stomach from overflowing, and keeps stomach
Interior voltage stabilizing；
（6）Simulate the digestion process of bionic-mouse stomach model and bionical duodenum model pipe：Initiation culture controller, it is electronic
Compression-rolling extrusion instrument starts vertically to compress the glandular stomach of bionical rat stomach model, while to glandular stomach from antrum
Toward the rolling extrusion in pylorus direction, gastric juice is pushed in bionic-mouse stomach model by single channel syringe pump A, is noted by single channel
It penetrates pump B pancreatic juice is pushed in bionical duodenum model pipe, bile, which is pushed to bionical 12, by single channel syringe pump C refers to
In intestines model pipe, pancreatic juice and bile and the chyme being discharged from bionic-mouse stomach model are transported to bionical duodenum by peristaltic pump
In model pipe, electric peristaltic squeezes instrument and carries out segmentation to bionical duodenum model pipe and wriggle to squeeze, finally by chyme from imitative
It is discharged in raw duodenum model pipe, is collected bottle collection；
（7）Time segment records digestion process：In step（6）In the digestion process, when digestion process is divided into several
Between section, stop a frequency controller, peristaltic pump, single channel syringe pump A, single channel syringe pump B and single-pass in each period
Road syringe pump C takes out bionic-mouse stomach model and bionical duodenum model pipe, collects bionic-mouse stomach model and bionical 12 respectively
Digest in duodenum 12 model pipe measures and records respectively its pH, maltose concentration, gastric emptying rate, storage modulus, loss mould
Chyme, is then put back to bionic-mouse stomach model and bionical ten by amount, dynamic viscosity and apparent viscosity, and observation microstructure again
In two duodenum 12 model pipes, frequency controller, compacted will be opened in bionic-mouse stomach model and bionical duodenum model pipe retrieval device
Dynamic pump, single channel syringe pump A, single channel syringe pump B and single channel syringe pump C, continue to test, until chyme is all from bionical ten
It is discharged in two duodenum 12 model pipes, and measures and record pH, maltose concentration, gastric emptying rate, the energy storage mould of final discharge chyme
Amount, loss modulus, dynamic viscosity and apparent viscosity, and observation microstructure；
（8）Clean bionic-mouse stomach model and bionical duodenum model pipe：After experiment, cleaned in time with deionized water
Bionic-mouse stomach model and bionical duodenum model pipe.
The step（7）Middle microstructure is observed by laser confocal microscope CSLM and scanning electron microscope sem.
Specific embodiment is lifted below for the present invention：
Embodiment 1：
Rice grain is given birth in rat stomach using dynamic bionic mouse stomach as shown in the figure-duodenum digestive system unit simulation
Mixing, grinding and digestion process, by detect gastric content pH, particle diameter distribution and digestion product-maltose concentration, and
It is compared with internal mouse experimental result living, to verify the validity of the device.Its specific operation process is as follows：
（1）According to apparatus of the present invention, apparatus of the present invention are assembled complete；
（2）Adjust the room temperature in incubation chamber：The heating lamp and digital display temperature controller in incubation chamber are opened, waits for the temperature in incubation chamber
When degree maintains 37 DEG C, heating lamp is closed；
（3）Filling gastric juice：A gastric juice pipe is taken, wherein filling full gastric juice, then by one end of gastric juice pipe and single channel
Syringe pump A is connected, and the other end is connected with pyloric canal；
（4）Prepare foodstuff samples：Weigh the grain of rice of 3.39 g difference initial particle ranges（d<0.6 mm:2.03 g；0.60
<d<0.85 mm：0.66 g；d>0.85 mm：0.70 g）With 2.15 g deionized waters in one 15 mL small beakers, then it is added
1.15 mL simulate rat saliva amylase solution（37℃）, 1 min of magnetic agitation (60 rpm), to simulate the grain of rice in rat mouthful
The mastication processes of intracavitary；
（5）Gastric juice is injected to bionic-mouse stomach model：0.6 mL simulate the gastric juices are injected to bionic-mouse stomach model with syringe, with
Gastric juice remains when simulating rat stomach fasting state；
（6）Food is injected to bionic-mouse stomach model：By step（4）In the food that is prepared disposably be injected into from esophagus
In bionic-mouse stomach model, silica gel valve pipe is connected with oesophagus outlet, prevents food in stomach from overflowing, and block pyloric canal, with
Digestion process when simulation food does not empty in stomach；
（7）Simulate digestion process of the food in bionic-mouse stomach model：Initiation culture controller, electric compressor-rolling are squeezed
Depressor tool starts vertically to compress the glandular stomach of bionical rat stomach model, while to glandular stomach from antrum toward pylorus direction
Gastric juice is pushed in bionic-mouse stomach model by rolling extrusion by single channel syringe pump A；
（8）Time segment records digestion process：In step（6）In the digestion process, digestion process is divided into four times
Section, be followed successively by start digest 30min, 60min, 120min and 180min, after each period stop frequency controller and
Single channel syringe pump A takes out bionic-mouse stomach model, and collects the digest in bionic-mouse stomach model, measures respectively and records it
PH, particle diameter distribution and maltose concentration；
（9）Clean bionic-mouse stomach model：After experiment, bionic-mouse stomach model is cleaned with deionized water in time.
Above-mentioned experimental result shows that the raw grain of rice is after digesting different time, pH, particle diameter distribution and the malt of gastric digestion object
Sugared concentration is consistent with internal mouse experimental result living, without significant difference（P>0.05）.Mouse living experiment and experiment in vitro in vivo
As a result it compares, pH is gradually decrease to 2.85 or so afterwards as digestion time increase first rises.After digesting 180 min, larger grain
Diameter（d>0.85 mm）Grain of rice ratio continuously decreased with the increase of digestion time, be reduced to 5% by initial 18%；Granule
Diameter (d<0.60 mm) ratio gradually rises, rise to 80% by initial 62%；Medium grain size（0.60<d<0.85 mm）Ratio
There is no significant change.
No matter live in the dynamic bionic mouse peptic digest system and device or in vivo in mouse experiment, maltose concentration with
The increase of digestion time is cumulative in serpentine, and the maltose concentration at 180 min moment is respectively 102 mg.mL-1With 125 mg.mL-1, but otherness is not notable（P>0.05）.And the relative increase of maltose concentration is protected with both variation tendencies of digestion time
It holds highly consistent.In addition, the relative error for the experimental data that experiment in vitro measures is significantly lower than experiment in vivo data.
Embodiment 2：
Contain the wheat of 10% pectin using dynamic bionic mouse stomach-duodenum digestive system unit simulation as shown in the figure
Starch granules rat stomach and it is duodenal mixing, grinding, digestion and evacuation procedure, by detect gastric content pH,
Maltose concentration, Starch Hydrolysis rate, gastric emptying rate, rheological property and microstructure, and to internal mouse experiment living and related
Result by references is compared, and to further verify the validity of the device, and probes into soluble dietary fiber pectin to wheat
Starch is in the influence that gastrointestinal tract is digested and empties.Its specific operation process is as follows：
（1）According to apparatus of the present invention, apparatus of the present invention are assembled complete；
（2）Adjust the room temperature in incubation chamber：The heating lamp and digital display temperature controller in incubation chamber are opened, waits for the temperature in incubation chamber
When degree maintains 37 DEG C, heating lamp is closed；
（3）Filling gastric juice, pancreatic juice and bile：A gastric juice pipe is taken, wherein filling full gastric juice, then by the one of gastric juice pipe
End is connected with single channel syringe pump A, and the other end is connected with pyloric canal, a pancreatic juice pipe and a bile pipe is separately taken, in pancreatic juice pipe
In filling full pancreatic juice, one end and the single channel syringe pump B of pancreatic juice pipe connect, the filling full bile in bile pipe, the one of bile pipe
End is connected with single channel syringe pump C, and the other end of pancreatic juice pipe is connected with the four-way pipe of the other end " K " word shape of bile pipe；
（4）Prepare foodstuff samples：
Control group：Take 3.5 g（Dry weight）Wheaten starch is placed in the container equipped with 6.5 mL deionized waters, is sufficiently stirred
Afterwards, then the solution that configuration dry matter content is 35% is added rat and simulates saliva, then magnetic agitation 1 minute (60 rpm),
To simulate food in the intraoral digestion process of mouse；
Experimental group：Take 3.5 g（Dry weight）Wheaten starch containing 10% pectin powder is placed in equipped with 6.5 mL deionized waters
In container, after being sufficiently stirred, then the solution that configuration dry matter content is 35% is added rat and simulates saliva, then magnetic agitation
1 minute (60 rpm), to simulate food in the intraoral digestion process of mouse；
（5）Gastric juice is injected to bionic-mouse stomach model：Simulate the gastric juice is injected to bionic-mouse stomach model with syringe, it is big to simulate
Gastric juice remains when mouse stomach fasting state；
（6）Foodstuff samples are injected to bionic-mouse stomach model：By step（4）In the control group that is prepared or experimental group food
Object sample is disposably injected into from esophagus in bionic-mouse stomach model, and silica gel valve pipe is connected with oesophagus outlet, is prevented in stomach
Food overflows, and keeps voltage stabilizing in stomach；
（7）Simulate the digestion process of bionic-mouse stomach model and bionical duodenum model pipe：Initiation culture controller, it is electronic
Compression-rolling extrusion instrument starts vertically to compress the glandular stomach of bionical rat stomach model, while to glandular stomach from antrum
Toward the rolling extrusion in pylorus direction, gastric juice is pushed in bionic-mouse stomach model by single channel syringe pump A, is noted by single channel
It penetrates pump B pancreatic juice is pushed in bionical duodenum model pipe, bile, which is pushed to bionical 12, by single channel syringe pump C refers to
In intestines model pipe, pancreatic juice and bile and the chyme being discharged from bionic-mouse stomach model are transported to bionical duodenum by peristaltic pump
In model pipe, electric peristaltic squeezes instrument and carries out segmentation to bionical duodenum model pipe and wriggle to squeeze, finally by chyme from imitative
It is discharged in raw duodenum model pipe, is collected bottle collection；
（8）Time segment records digestion process：In step（7）In the digestion process, digestion process is divided into eight times
Section, is followed successively by and starts digestion 10,20,30,40,60,90,120 and 180 min, stops a FREQUENCY CONTROL in each period
Device, peristaltic pump, single channel syringe pump A, single channel syringe pump B and single channel syringe pump C take out bionic-mouse stomach model and bionical ten
Two duodenum 12 model pipes collect the digest in bionic-mouse stomach model and bionical duodenum model pipe respectively, measure respectively its pH,
Maltose concentration, Starch Hydrolysis rate, gastric emptying rate, rheological property（Storage modulus, loss modulus, dynamic viscosity and apparent
Viscosity）And microstructure, after experiment, close frequency controller, peristaltic pump, single channel syringe pump A, single channel syringe pump B
With single channel syringe pump C；
（9）Clean bionic-mouse stomach model and bionical duodenum model pipe：After experiment, cleaned in time with deionized water
Bionic-mouse stomach model and bionical duodenum model pipe.
Above-mentioned experiment shows, be added 10% mango powder experimental group and its gastric digestion object pH of control group foodstuff samples with
It the increase of digestion time and first increases and continuously decrease afterwards, and the digested object pH of duodenum remains at 7 or so.Experimental group
Maltose concentration, Starch Hydrolysis rate and the gastric emptying rate of digest are substantially less than control group, but storage modulus, loss mould
Amount, dynamic viscosity and apparent viscosity are significantly higher than control group（P<0.05）.Microstructure observing result shows that pectin passes through to be formed
Gel networks wrap up starch granules inside it, effective contact of digestive ferment and substrate are prevented, to reduce starch
Percent hydrolysis.Dietary fiber-pectin that above-mentioned experimental result is reported with internal mouse experiment living and pertinent literature result can pass through
Increase food viscosity and form gel networks and wraps up starch granules to reduce mixing, gastric emptying rate and the starch of food
Digestibility is consistent.
The technical means disclosed in the embodiments of the present invention is not limited to the technical means disclosed in the above technical means, and further includes
By the above technical characteristic arbitrarily the formed technical solution of combination.
It is enlightenment with above-mentioned desirable embodiment according to the present invention, through the above description, relevant staff is complete
Various changes and amendments can be carried out without departing from the scope of the technological thought of the present invention' entirely.The technology of this invention
Property range is not limited to the contents of the specification, it is necessary to determine its technical scope according to right.
Claims (4)
1. bionical dynamic mouse stomach-duodenum digestive system simulator, the simulator is for simulating food in the stomach of mouse
Digestion after Gastric juice digestion using pancreatic juice and bile in duodenum, for studying food in digestion process and final
Gruel variation, it is characterized in that disappearing including peptic digest system, duodenum digestive system, stream plus-emptier and incubation chamber, the stomach
Change system, duodenum digestive system and stream plus-emptier is respectively positioned in incubation chamber, stream plus-emptier are towards peptic digest
System conveys gastric juice, and bile and pancreatic juice, peptic digest system and duodenum digestive system are conveyed towards duodenum digestive system
It is interconnected；
The peptic digest system includes bionic-mouse stomach model and electric compressor-rolling extrusion instrument, in the bionic-mouse stomach model
Equipped with foodstuff samples, the electric compressor-rolling extrusion instrument is used for rolling extrusion bionic-mouse stomach model, the production of bionic-mouse stomach model
Raw simulation stomach contraction-rolling digestion action；
The duodenum digestive system includes that bionical duodenum model pipe and electric peristaltic squeeze instrument, the bionic-mouse stomach
Foodstuff samples in model are discharged into after the digestion of bionic-mouse stomach model in bionical duodenum model pipe, the electric peristaltic
It squeezes instrument and squeezes bionical duodenum model pipe for wriggling, bionical duodenum model pipe generates simulation duodenum and wriggles
The action of digestion；
The stream plus-emptier include single channel syringe pump A, single channel syringe pump B and single channel syringe pump C, the single-pass
Road syringe pump A is connected to bionic-mouse stomach model by gastric juice pipe, single channel syringe pump A towards conveying gastric juice in bionic-mouse stomach model,
The single channel syringe pump B is connected to bionical duodenum model pipe by pancreatic juice pipe, and single channel syringe pump C is connected to by bile pipe
Bionical duodenum model pipe, single channel syringe pump B is towards conveying pancreatic juice, single channel syringe pump in bionical duodenum model pipe
C is towards conveying bile in bionical duodenum model pipe；
The free end of the bionical duodenum model pipe is provided with chyme receiving flask, and the chyme receiving flask is for collecting from imitative
The chyme being discharged in raw duodenum model pipe；
The bionic-mouse stomach model is to make to be molded by overmolded using the mouse stomach of true full abdomen size, uses " Dragon
Skin " addition-type silicon rubbers are made, size, shape and internal structure and the true rat of the bionic-mouse stomach model
Stomach is consistent, using boundary ridge as boundary, is divided into glandular stomach and glandular stomach, glandular stomach inner wall smooth, glandular stomach inner wall is full of fold, glandular stomach connection food
It manages, silica gel valve pipe is installed in oesophagus, glandular stomach is connected to pyloric canal and gastric secretion pipe, and the gastric secretion pipe is connected to single channel
Syringe pump A, the pyloric canal are connected to bionical duodenum model pipe, and food enters bionic-mouse stomach model from oesophagus, through bionic-mouse
After stomach model digestion bionical duodenum model pipe is expelled to from pyloric canal；
The length and internal diameter of the bionical duodenum model pipe are equal with the duodenal length of true mouse and internal diameter size, imprison
Door pipe is connected with bionical duodenum model pipe by the four-way pipe of " K " word shape, " I " of the four-way pipe of " K " word shape
Partial one end is connected with pyloric canal, and the other end is connected with bionical duodenum model pipe, the four-way pipe of " K " word shape
One end of the part " ＜ " is connected by pancreatic juice pipe with single channel syringe pump B, and the other end passes through bile pipe and single channel syringe pump C phases
Even；
The electric compressor-rolling extrusion instrument includes stepper motor, holder, eccentric wheel, frequency controller, gusset, belt, skin
Belt wheel, bevel gear, pressing plate, fixed plate, support plate and pedestal, the holder are fixed on the base, and two are provided on the holder
A fixed plate, is fixed with support plate between described two fixed plates, the bionic-mouse stomach model installation on the supporting plate, Mei Gegu
There are one stepper motors for installation on fixed board, and by bevel gear connecting eccentric wheel, eccentric wheel rotated for an end of stepper motor
Compression vertical up and down is carried out to glandular stomach with dynamic pressure plate in journey, the fixed plate is additionally provided with three belts of distribution triangular in shape
It takes turns, belt is cased on the belt pulley, one of belt pulley has stepper motor driving, stepper motor to drive belt and then drive
Eccentric wheel carries out from antrum toward pylorus direction rolling extrusion glandular stomach, and the frequency controller is connect with stepper motor, frequency control
Device processed is compressed adjusting bionic-mouse stomach model by controlling the rotating speed of stepper motor and rolling extrusion frequency and amplitude；
The electric peristaltic squeeze instrument include stepper motor, eccentric wheel, frequency controller, bevel gear, fast pulley, support plate,
Fixed plate, belt, belt pulley and pedestal, the holder are fixed on the base, and fixed plate, the fixed plate are fixed on the holder
Upper several fast pulleys of setting, the bionical duodenum model pipe are curved through each fast pulley successively, the fixed plate
Side is provided with two stepper motors, and every stepper motor is by bevel gear connecting eccentric wheel, and the eccentric wheel is to imitative
Raw duodenum model pipe carries out subsection interleaving formula wriggling and squeezes, and the frequency controller connects stepper motor, frequency controller
Frequency is squeezed to the wriggling of bionical duodenum model pipe to adjust eccentric wheel by controlling the rotating speed of stepper motor.
2. bionical dynamic mouse stomach-duodenum digestive system simulator according to claim 1, it is characterized in that described imitative
The chyme upstream end of raw duodenum model pipe is provided with peristaltic pump.
3. bionical dynamic mouse stomach-duodenum digestive system simulator according to claim 1, it is characterized in that the guarantor
It is provided in warm box for the heating lamp of air in heating heat-preserving box and for carrying out illumination headlamp, the guarantor in incubation chamber
The digital display temperature controller for monitoring the real time temperature inside incubation chamber is additionally provided in warm box, the side of the incubation chamber is provided with
Sliding door.
4. the analogue experiment method of any of the above-described bionical dynamic mouse stomach-duodenum digestive system simulator of application,
It is characterized in that including the following steps：
（1）Adjust the room temperature in incubation chamber：The heating lamp and digital display temperature controller in incubation chamber are opened, the temperature in incubation chamber is always
37 DEG C are maintained, when temperature is higher than 37 DEG C, will turn off heating lamp, when being less than 37 DEG C, heating lamp then automatically turns on；
（2）Filling gastric juice, pancreatic juice and bile：Take a gastric juice pipe, wherein filling full gastric juice, then by one end of gastric juice pipe with
Single channel syringe pump A is connected, and the other end is connected with pyloric canal, is separately taken a pancreatic juice pipe and a bile pipe, is filled in pancreatic juice pipe
Fill pancreatic juice, one end and the single channel syringe pump B of pancreatic juice pipe are connected, the filling full bile in bile pipe, one end of bile pipe with
Single channel syringe pump C is connected, and the other end of pancreatic juice pipe is connected with the four-way pipe of the other end " K " word shape of bile pipe；
（3）Prepare foodstuff samples：It takes food and is placed in the container equipped with deionized water, after being sufficiently stirred, mouse saliva is added, then
Magnetic agitation, to simulate food in the intraoral digestion process of mouse；
（4）Gastric juice is injected to bionic-mouse stomach model：Simulate the gastric juice is injected to bionic-mouse stomach model with syringe, to simulate rat stomach
Gastric juice remains when fasting state；
（5）Foodstuff samples are injected to bionic-mouse stomach model：By step（3）In the foodstuff samples that are prepared disposably noted from esophagus
It is mapped in bionic-mouse stomach model, silica gel valve pipe is connected with oesophagus outlet, prevents food in stomach from overflowing, and keeps steady in stomach
Pressure；
（6）Simulate the digestion process of bionic-mouse stomach model and bionical duodenum model pipe：Initiation culture controller, electronic pressure
Contracting-rolling extrusion instrument starts vertically to compress the glandular stomach of bionical rat stomach model, while past from antrum to glandular stomach
Gastric juice is pushed in bionic-mouse stomach model by single channel syringe pump A, is injected by single channel by the rolling extrusion in pylorus direction
Pancreatic juice is pushed in bionical duodenum model pipe by pump B, and bile is pushed to bionical duodenum by single channel syringe pump C
In model pipe, pancreatic juice and bile and the chyme being discharged from bionic-mouse stomach model are transported to bionical duodenum mould by peristaltic pump
In type pipe, electric peristaltic squeezes instrument and carries out segmentation to bionical duodenum model pipe and wriggle to squeeze, finally by chyme from bionical
It is discharged in duodenum model pipe, is collected bottle collection；
（7）Time segment records digestion process：In step（6）In the digestion process, digestion process is divided into several periods,
Stop a frequency controller, peristaltic pump, single channel syringe pump A, single channel syringe pump B and single channel injection in each period
C is pumped, bionic-mouse stomach model and bionical duodenum model pipe are taken out, collects bionic-mouse stomach model and bionical duodenum mould respectively
Digest in type pipe measures and records respectively its pH, maltose concentration, gastric emptying rate, storage modulus, loss modulus, moves
Then chyme is put back to bionic-mouse stomach model and bionical 12 and referred to by state viscosity and apparent viscosity, and observation microstructure again
In intestines model pipe, frequency controller in bionic-mouse stomach model and bionical duodenum model pipe retrieval device, will be opened, wriggled
Pump, single channel syringe pump A, single channel syringe pump B and single channel syringe pump C, continue to test, until chyme is all from bionical 12
Be discharged in duodenum 12 model pipe, and measure and the pH of the final discharge chyme of record, maltose concentration, gastric emptying rate, storage modulus,
Loss modulus, dynamic viscosity and apparent viscosity, and observation microstructure, microstructure pass through laser confocal microscope CSLM
It is observed with scanning electron microscope sem；
（8）Clean bionic-mouse stomach model and bionical duodenum model pipe：After experiment, cleaned in time with deionized water bionical
Mouse stomach model and bionical duodenum model pipe.
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