CN105639204A - Biological color enhancing agent and application thereof to aquatic animal breeding - Google Patents
Biological color enhancing agent and application thereof to aquatic animal breeding Download PDFInfo
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- CN105639204A CN105639204A CN201610031287.3A CN201610031287A CN105639204A CN 105639204 A CN105639204 A CN 105639204A CN 201610031287 A CN201610031287 A CN 201610031287A CN 105639204 A CN105639204 A CN 105639204A
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- phaffia rhodozyma
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- red phaffia
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- Fodder In General (AREA)
Abstract
The invention discloses a biological color enhancing agent which is prepared from 60-80 parts of Phaffia rhodozyma spray dry powder, 8-12 parts of Phaffia rhodozyma freeze-dried powder, 8-12 parts of a lactic acid bacteria fermentation product, 4-8 parts of antimicrobial peptide, 2-4 parts of mountain flour and 0.5-1.5 parts of glutamine. The invention further discloses a preparation method of the biological color enhancing agent and application of the biological color enhancing agent to aquatic animal breeding. According to the biological color enhancing agent, Phaffia rhodozyma serves as the effective ingredient, the nutritious value is rich, the content of protein is high, and the biological color enhancing agent is rich in multiple types of microelements and coenzyme, further rich in astaxanthin and configured with multiple types of nutrient substances and has the advantage of being stable in color enhancing effect in serving as the color enhancing agent for aquatic animal breeding.
Description
Technical field
The present invention relates to a kind of biological toner and the application in aquaculture of aquatic animal thereof, belong to aquaculture of aquatic animal technical field.
Background technology
Along with the fast development of artificial cultivation industry, the transition of environment, the change of food chain, the body colour of aquatic animal is made to there occurs change in various degree. The fish and shrimp grown in its natural state are bright in luster, delicious meat, and under artificial intensive cultivating condition, owing to using artifical compound feed in a large number, fish, shrimp, Eriocheir sinensis culture-cycle shorten, a small amount of natural aquatic can only be obtained biological, and effective color source is few and composition is unstable in feedstuff, cause fish, shrimp, Eriocheir sinensis body colour thin out, channel catfish " bleaches ", " becoming flower " even " blackening "; Yellow wood fish " bleaches ", " blackening "; Monopterus albus (Zuiew), Misgurni anguillicaudati " rubescent "; Bream becomes " Carassius auratus "; Cyprinus carpio, crucian carp " blackening ", " reddening "; Mylopharyngodon piceus " turns white "; Ctenopharyngodon idellus " golden yellow " etc. These abnormal body colours reduce the commodity value of cultured fishes, bring economic loss to culturist. Therefore, how to improve the body colour of Aquatic farming animals, so as to be close with the body colour of wild fish and shrimp, be always up in aquaculture field important research and put into practice problem.
The speckle billows color that aquatic animal can present, is mainly determined by carotenoid, also relevant with the pigment group such as melanin, guanine, but aquatic animal itself can not synthesize carotenoid, thus must absorb carotenoid from food. Coloring agent is by sources generally divided into two kinds, i.e. natural colorant and synthetic coloring matter. Natural colorant refers to that some are yellow rich in carotenoid and phylloxanthin etc., red, the animal of purple and extract thereof, such as oppossum shrimp, squama shrimp etc.; Plant and extract thereof, such as Semen Maydis, Radix Dauci Sativae, lucerne potato starch, Pericarpium Citri tangerinae etc.; Microorganism and extract thereof, the rhodospirillum in yeast, photosynthetic bacteria, the Dunaliella salina etc. in miniature algae; Spirulina and extract thereof. Under modern intensive cultivating condition, the ingest chance of natural bait (rich in carotenoid) of aquatic animal is fewer and feweri, therefore, adds carotenoid as coloring agent in feedstuff, cultured fishes body colour is regulated and controled, has caused the extensive attention of research worker.
Summary of the invention
For above-mentioned prior art, it is an object of the invention to provide a kind of biological toner and the application in aquaculture of aquatic animal thereof. This biology toner with red phaffia rhodozyma for effective ingredient, rich in nutritive value, protein content is high and rich in various trace elements and coenzyme, also rich in astaxanthin, and it is equipped with multiple nutrients material, as aquaculture of aquatic animal toner, the advantage with hyperchromic effect stability.
For achieving the above object, the present invention adopts following technical proposals:
A kind of biological toner, its raw material consists of:
Red phaffia rhodozyma spray powder 60-80 part, red phaffia rhodozyma lyophilized powder 8-12 part, lactic acid bacteria fermentation product 8-12 part, antibacterial peptide 4-8 part, stone powder 2-4 part, glutamine 0.5-1.5 part.
Preferably, the raw material of this biology toner consists of:
Red phaffia rhodozyma spray powder 70 parts, red phaffia rhodozyma lyophilized powder 10 parts, lactic acid bacteria fermentation product 10 parts, antibacterial peptide 6 parts, stone powder 3 parts, glutamine 1 part.
Preferably, described red phaffia rhodozyma spray powder and red phaffia rhodozyma lyophilized powder, it is in yeast culture medium, carried out fermentation culture by red phaffia rhodozyma to obtain fermentation liquid, fermentation liquid is prepared from through spray drying and lyophilizing respectively.
Described red phaffia rhodozyma is that strain number is CICC33064, has the yeast strain of high-yield astaxanthin function purchased from Chinese industrial Microbiological Culture Collection administrative center.
The raw material of described yeast culture medium consists of: glucose 1.5%, peptone 1.0%, yeast extract 0.5%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.02%, sodium chloride 2.0%, calcium chloride 0.02%, glutamic acid 0.06%, is mass percent. The composition of yeast culture medium is optimized screening by the present invention, finds after great many of experiments contrasts, and adopting the yeast culture medium of above-mentioned composition, the amount of red Astaxanthin Production by Phaffia rhodozyma and Biomass are all higher, but also reduce production cost.
Preferably, described lactic acid bacteria fermentation product is the tunning that Pediococcus pentosaceus PP (PediococcuspentosaceusPP) fermented cultivation obtains; Pediococcus pentosaceus PP was preserved in China typical culture collection center on 02 22nd, 2012, and its deposit number is CCTCCNO:M2012029; It is documented in a patent of invention " a kind of lactic acid bacteria semisolid fermentation product improving growth of animal performance and technique " (CN102599348B) of applicant. Above-mentioned Pediococcus pentosaceus is a kind of important probiotics, and body has important physiological action. Its growth metabolism process producing lactic acid, bacteriocin etc., reducing intestinal pH, thus suppressing the growth and breeding of pathogenic bacterium; Intestinal epithelial cell can also be attached to simultaneously, fight for limited nutrient substance and ecological site with pathogenic microorganism, play occupancy protective effect etc. This bacterial strain overcomes the drawback of external environment tolerance difference, can pass through the low pH in digestive tract as effective microbial ecological agent and have stronger colonization ability and play its biological function. Above-mentioned Pediococcus pentosaceus is applied in biological toner by the present invention, it is possible to improve the internal microecological environment of aquatic animal, safeguards that digestive tract is healthy.
The preparation method that the present invention also provides for this biology toner, step is: by each component mix homogeneously, to obtain final product.
This biology toner is also protection scope of the present invention as the application of hyperchromic feed additive in aquaculture of aquatic animal.
In above-mentioned application, concrete application process is: added in aquatic animal feed in the ratio that mass fraction is 1-3 �� by this biology toner, within 30 days, is a cycle or throw something and feed for a long time of throwing something and feeding.
The present invention also provides for a kind of hyperchromic feedstuff of aquaculture of aquatic animal, is by being that 1-3 �� adds above-mentioned biology toner and is prepared from by mass fraction in aquatic animal normal feedstuff.
Beneficial effects of the present invention:
(1) the biological toner of the present invention is with red phaffia rhodozyma for effective ingredient, rich in nutritive value, protein content is high and rich in various trace elements and coenzyme, also rich in astaxanthin, and it is equipped with multiple nutrients material, can as aquaculture of aquatic animal toner, the advantage with hyperchromic effect stability.
(2), in the biological toner of the present invention, red phaffia rhodozyma adds with lyophilized powder and two kinds of forms of spray powder, and wherein, the viable count in lyophilized powder is high, but preparation cost is also high; In spray powder, viable count is relatively low, but preparation cost is low simultaneously to meet nutritional need. Considering the viable count in biological toner and production cost, the addition of red phaffia rhodozyma lyophilized powder and spray powder is optimized by the present invention, makes to obtain good balance between hyperchromic effect and the preparation cost of biological toner.
(3) in the biological toner of the present invention, the kind of red phaffia rhodozyma has been screened, preferably being preserved in Chinese industrial Microbiological Culture Collection administrative center, strain number is the red phaffia rhodozyma of CICC33064, and its ability producing astaxanthin is significantly better than other bacterial strains.
(4) in the biological toner of the present invention rich in astaxanthin directly can have been utilized pigment accumulation process by aquatic animal, directly play hyperchromic effect, on the other hand its rich in nutrient substance ensure that the nutritional need of aquatic animal improves the immunity of aquatic animal, the antibacterial peptide added also is able to play the effect improving body resistance against diseases, glutamine can play the effect safeguarding that digestive tract is healthy simultaneously, is that aquatic animal creates the optimum condition safeguarding body colour from the angle of nutrition and raising immunity. The biological toner of the present invention acts on body color of aquatic animal from directly or indirectly two aspects and safeguards, effect is significantly lasting.
Accompanying drawing explanation
Scale sector area under Fig. 1: 10x mirror;
Pigment cell under Fig. 2: 40x mirror;
Fig. 3: at albefaction coloured silk crucian carp fin ray base portion, pigment accumulation occurs after the 42d that throws something and feeds;
Detailed description of the invention
The present invention is further illustrated in conjunction with the embodiments, it should explanation, and its content, merely to explain the present invention, is not defined by the description below.
Embodiment 1: different yeast strains produce astaxanthin ability and compare the formulation optimization with yeast culture medium
1 materials and methods
1.1 test strains
Rhodotorula glutinis (N), deep rhodotorula glutinis (SN), ocean rhodotorula (H), Rhodothece glutinis (R8) are the existing yeast of biological study institute of Shandong Baolai-leelai Bio-engineering Co., Ltd..
Red phaffia rhodozyma (R5) is that strain number is CICC33064 purchased from Chinese industrial Microbiological Culture Collection administrative center, has the yeast strain producing astaxanthin function.
1.2 different yeast strains produce astaxanthin ability and compare
Using the basic components of yeast culture medium as fermentation medium, different yeast strains is carried out fermentation culture, adopt dimethyl sulfoxide method to measure its ability producing astaxanthin.
The basic components of yeast culture medium: glucose 1.5%, peptone 1.0%, yeast extract 0.5%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.01%, sodium chloride 1.0%; It is mass percent.
The formulation optimization of 1.3 yeast culture medium
With astaxanthin and Biomass for evaluation index, formed by the formula of optimization of orthogonal test yeast culture medium.
The assay method of astaxanthin: dimethyl sulfoxide method;
The assay method of Biomass: after fermentation liquid is washed three times with distilled water centrifugal (4000r/min, 15min), dries 24 hours, weighs for 105 DEG C, unit mg/mL.
Table 1 orthogonal design formula forms
The screening of 1.4 wall breaking enzymes
Adopt combined-enzyme method breaking cellular wall compared with the astaxanthin that dimethyl sulfoxide method measures, it is determined that sporoderm-broken rate.
Table 2 breaking cellular wall enzyme orthogonal design formula
2 result of the tests
2.1 different saccharomycetic product astaxanthin abilities
Table 3 bacterial strain produces the screening of astaxanthin ability
As can be seen from Table 3, strain number is that the product astaxanthin ability of the red phaffia rhodozyma (R5) of CICC33064 is substantially better than other yeast strains.
Table 4 Biomass and astaxanthin yield change over trend
As can be seen from Table 4, each yeast steps up along with the amount extending synthesizing astaxanthin of incubation time, but red phaffia rhodozyma (R5) aggregate velocity is significantly faster than that other bacterial strains, therefore R5 is ultimately determined to the aimed strain for preparing biological toner.
The formulation optimization result of 2.2 yeast culture medium
Table 5 formulation optimization result
As shown in Table 5, formula 5 and formula 7 effect are better, but formula 5 on cost lower than formula 7, therefore the formula finally determining yeast culture medium is formula 5, namely glucose 1.5%, peptone 1.0%, yeast extract 0.5%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.02%, sodium chloride 2.0%, calcium chloride 0.02%, glutamic acid 0.06%, be mass percent.
The selection result of 2.3 wall breaking enzymes
Table 6 compound enzyme breaking cellular wall result
As shown in Table 6, the sporoderm-broken rate of formula 5 is up to 85%, and effect is better, it is determined that it is the prescription of compound wall breaking enzyme.
Embodiment 2: the preparation of biological toner
1 raw material composition
It is made up of the raw material of following weight portion:
Red phaffia rhodozyma spray powder 70 parts, red phaffia rhodozyma lyophilized powder 10 parts, lactic acid bacteria fermentation product 10 parts, antibacterial peptide 6 parts, stone powder 3 parts, glutamine 1 part.
Wherein, red phaffia rhodozyma spray powder and red phaffia rhodozyma lyophilized powder, it is in yeast culture medium, carried out fermentation culture by red phaffia rhodozyma to obtain fermentation liquid, fermentation liquid is prepared from through spray drying and lyophilizing respectively. Fermentation culture and spray drying, lyophilizing operation are routine techniques means of the prior art.
Red phaffia rhodozyma is that strain number is CICC33064 purchased from Chinese industrial Microbiological Culture Collection administrative center, has the yeast strain producing astaxanthin function.
The raw material of yeast culture medium consists of: glucose 1.5%, peptone 1.0%, yeast extract 0.5%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.02%, sodium chloride 2.0%, calcium chloride 0.02%, glutamic acid 0.06%, is mass percent.
Lactic acid bacteria fermentation product is the tunning that Pediococcus pentosaceus PP (PediococcuspentosaceusPP) fermented cultivation obtains; Pediococcus pentosaceus PP was preserved in China typical culture collection center on 02 22nd, 2012, and its deposit number is CCTCCNO:M2012029.
Antibacterial peptide is the product of Shandong Baolai-leelai Bio-engineering Co., Ltd., trade name: antibacterial peptide CEC-38.
2 preparation methoies
Weigh raw material, mix homogeneously by above-mentioned weight portion, to obtain final product.
Embodiment 3: the preparation of biological toner
Raw material forms:
Red phaffia rhodozyma spray powder 60 parts, red phaffia rhodozyma lyophilized powder 12 parts, lactic acid bacteria fermentation product 8 parts, antibacterial peptide 8 parts, stone powder 2 parts, glutamine 1.5 parts.
Preparation method is with embodiment 2.
Embodiment 4: the preparation of biological toner
Raw material forms:
Red phaffia rhodozyma spray powder 80 parts, red phaffia rhodozyma lyophilized powder 8 parts, lactic acid bacteria fermentation product 12 parts, antibacterial peptide 4 parts, stone powder 4 parts, glutamine 0.5 part.
Preparation method is with embodiment 2.
Embodiment 5: biological toner application in aquaculture of aquatic animal
1 materials and methods
The raising of 1.1 experimental animals
Test color crucian carp, average weight (9 �� 0.5) g, support temporarily in indoor aquarium (0.9m �� 0.5m �� 0.5m), and effective water volume is 100L. The tap water dechlorinated using abundant aeration is as water source, and water temperature controls in (24 �� 1) DEG C, and 24h continues oxygenation; Day, feeding volume was the 1.5% of fish body weight, and early, middle and late timing is thrown something and fed. After the adaptive training of 14d, test fish is randomly divided into 4 groups by body weight, respectively matched group (CK), test group I (Y I), test group II (Y II), competing product group (L), often organize 25 tails, sample respectively at 14d, 28d, 42d, 56d, 60d.
The addition of 1.2 toners two dosage groups of biological toner of the embodiment of the present invention 2 preparation (Y I, the Y II are)
The biological toner addition (per kilogram basal diet) of table 7
The competing product group of note: L is the sterling of chemosynthesis astaxanthin.
The adding method of 1.3 toners
By the addition of table 7, will being uniformly sprayed onto in the middle of color crucian carp complete feed after toner water dissolution, ventilation preserves to 4 DEG C of refrigerators after half-dried.
1.4 indexs measured
Chromogenic indicator: skin, scale, fin ray total carotinoid mensuration, the mensuration of skin colourity, pigment cell count;
Growth indexes: body weight, liver body index;
Immune performance: index and spleen index, middle renal index;
1.5 index determining methods
Liver body index: liver pancreas weight/body weight;
Index and spleen index: spleen weight/body weight;
Pigment cell counts: finds pigment cell under microscope 40x, adds up pigment cell's number under a visual field and count.
Colourity: adopt colorimetric card colorimetric, records chromatic value;
The mensuration of astaxanthin: dimethyl sulfoxide method;
2 result of the tests
2.1 chromogenic indicator
2.1.1 total carotinoid
Scale, skin, fin ray total carotinoid assay result, in Table 8.
Table 8 scale, skin, fin ray total carotinoid content
Note: same column has same letter representation difference not notable (P > 0.05), has different letter representation significant difference (P < 0.05).
As can be seen from Table 8, the accumulation law of total carotinoid is first reduce then to rise by a small margin by scale again, and when 14d, test group I, test group II, competing product group and matched group, all without significant difference, are all remarkably higher than matched group when 60d; The significance relation of total carotinoid is occurred in 28d by skin, only adds biological toner group and be significantly higher than matched group during to 60d; The significance relation of fin ray total carotinoid also appears in 28d, only adds high dose biology toner group to 60d and is significantly higher than matched group. Analysis reason is probably early stage and renders in albefaction bucket, owing to Fish have automatic regulation of the body color mechanism, is similar to the sematic color of animal; the color of automatic closeness to life environment; cause pigment accumulation ability to decline, albefaction trend occurs, owing to taking a turn for the better occurs in the long-term interpolation situation of pigment; but due to when 42d; time enters mid-November, and temperature begins to decline, minimizing of ingesting; the biological toner etc. taken in all reduces, so pigment accumulation has on a declining curve. Totally, comparison between each group, biological toner interpolation group effect is better than competing product group, effect stability, the astaxanthin that competing product group is added belongs to chemosynthesis astaxanthin, though self has whitening power, but the astaxanthin composition of chemosynthesis is single, and building-up process has some harmful substances that cannot remove, life-time service can affect fish body function, causes pigment accumulation weak effect.
2.1.2 skin colourity and pigment cell's counting
Table 9 skin colourity and pigment cell count average
As can be seen from Table 9, throwing something and feeding through 60d, test group I (Y I), the skin colourity average of test group II (Y II), pigment cell's average are above matched group;From Fig. 1, Fig. 2 it is also seen that pigment cell is individual and pigment cell's density is above matched group, the sector area formed, also greater than matched group, illustrates that this product can promote that pigment cell grows, breeds.
2.2 growth indexes
2.2.1 body weight
Body weight change respectively organized by table 10
As can be seen from Table 10, test group I, test group II, competing product group and matched group there are no significant difference, but test group I and test group II body weight are the trend continuing to rise, only competing product group and matched group occur in that a flex point, illustrating to add no matter biological toner group is all can better improve body weight and effect stability at low dosage or high dose group, high dose biology toner group effect is better than low dose group.
2.2.2 liver body index
Liver body index variation respectively organized by table 11
As can be seen from Table 11, test group I, test group II, competing product group liver body index and matched group there are no significant difference, but all in continuing the trend that rises, and biological toner high dose group effect optimum.
2.3 immune indexes
Immune indexes respectively organized by table 12
As can be seen from Table 12, test group I, test group II, competing product group and matched group there are no significant difference, add biological toner group effect and be slightly better than competing product group and matched group, the possible cause that flex point occurs in competing product group is: synthetic astaxanthin contains by-product, it is impaired that the life-time service of by-product may result in fish body immune organ, limits Development of Immune Organs. And the natural astaxanthin that biology toner self contains and nutritional labeling can promote Development of Immune Organs, play the effect improving immunity of organisms.
2.4 digestion indexs
2.4.1 intestinal digestive enzyme
The change of intestinal digestive enzyme respectively organized by table 13
As can be seen from Table 13, add biological toner group when 60d, proteinase activity is significantly higher than matched group and competing product group, amylase only adds high dose biology toner group and is significantly higher than other each group, illustrates that adding biological toner can significantly improve digestive enzyme activity and notable with dosage increase effect.
Claims (10)
1. a biological toner, it is characterised in that its raw material consists of:
Red phaffia rhodozyma spray powder 60-80 part, red phaffia rhodozyma lyophilized powder 8-12 part, lactic acid bacteria fermentation product 8-12 part, antibacterial peptide 4-8 part, stone powder 2-4 part, glutamine 0.5-1.5 part.
2. biological toner as claimed in claim 1, it is characterised in that its raw material consists of:
Red phaffia rhodozyma spray powder 70 parts, red phaffia rhodozyma lyophilized powder 10 parts, lactic acid bacteria fermentation product 10 parts, antibacterial peptide 6 parts, stone powder 3 parts, glutamine 1 part.
3. biological toner as claimed in claim 1 or 2, it is characterized in that, described red phaffia rhodozyma spray powder and red phaffia rhodozyma lyophilized powder, be carried out fermentation culture in yeast culture medium by red phaffia rhodozyma to obtain fermentation liquid, be prepared from through spray drying and lyophilizing respectively by fermentation liquid.
4. biological toner as claimed in claim 3, it is characterised in that described red phaffia rhodozyma is the yeast strain of high-yield astaxanthin function, and strain number is CICC33064.
5. biological toner as claimed in claim 3, it is characterized in that, the raw material of described yeast culture medium consists of: glucose 1.5%, peptone 1.0%, yeast extract 0.5%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.02%, sodium chloride 2.0%, calcium chloride 0.02%, glutamic acid 0.06%; It is mass percent.
6. biological toner as claimed in claim 1 or 2, it is characterised in that described lactic acid bacteria fermentation product is the tunning that Pediococcus pentosaceus PP (PediococcuspentosaceusPP) fermented cultivation obtains;The deposit number of Pediococcus pentosaceus PP is CCTCCNO:M2012029.
7. the preparation method of the biological toner described in any one of claim 1-6, it is characterised in that by red phaffia rhodozyma spray powder, red phaffia rhodozyma lyophilized powder, lactic acid bacteria fermentation product, antibacterial peptide, stone powder and glutamine mix homogeneously, to obtain final product.
8. the biological toner described in any one of claim 1-6 is applied in aquaculture of aquatic animal.
9. apply as claimed in claim 8, it is characterised in that application process is: the biological toner described in any one of claim 1-6 is added in aquatic animal feed in the ratio that mass fraction is 1-3 ��, within 30 days, be a cycle or throw something and feed for a long time of throwing something and feeding.
10. the hyperchromic feedstuff of aquaculture of aquatic animal, is by being that the 1-3 �� biological toner added described in any one of claim 1-6 is prepared from by mass fraction in aquatic animal normal feedstuff.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107343538A (en) * | 2017-06-21 | 2017-11-14 | 青海省畜牧兽医科学院 | A kind of yak yoghourt powder and preparation method thereof |
| CN108094754A (en) * | 2017-11-24 | 2018-06-01 | 镇江华优生态农业科技有限公司 | A kind of young extremely detains the mixed feed in crab stage and preparation method thereof |
| CN112515053A (en) * | 2020-11-02 | 2021-03-19 | 无锡泓宝微生物科技有限公司 | A QINGSU bacterium for enhancing pigment and immunity of aquatic animal |
| CN112617027A (en) * | 2020-12-31 | 2021-04-09 | 上海泓宝绿色水产股份有限公司 | Bdelloecium bdellovii and preparation method and use method thereof |
| CN114431333A (en) * | 2021-12-24 | 2022-05-06 | 青岛尚德生物技术有限公司 | Phaffia rhodozyma liquid composite preparation and preparation method and application thereof |
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| CN103865798A (en) * | 2014-03-18 | 2014-06-18 | 厦门汇盛生物有限公司 | Method for wall breaking of phaffia rhodozyma through enzymolysis and application thereof |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103865798A (en) * | 2014-03-18 | 2014-06-18 | 厦门汇盛生物有限公司 | Method for wall breaking of phaffia rhodozyma through enzymolysis and application thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107343538A (en) * | 2017-06-21 | 2017-11-14 | 青海省畜牧兽医科学院 | A kind of yak yoghourt powder and preparation method thereof |
| CN108094754A (en) * | 2017-11-24 | 2018-06-01 | 镇江华优生态农业科技有限公司 | A kind of young extremely detains the mixed feed in crab stage and preparation method thereof |
| CN112515053A (en) * | 2020-11-02 | 2021-03-19 | 无锡泓宝微生物科技有限公司 | A QINGSU bacterium for enhancing pigment and immunity of aquatic animal |
| CN112617027A (en) * | 2020-12-31 | 2021-04-09 | 上海泓宝绿色水产股份有限公司 | Bdelloecium bdellovii and preparation method and use method thereof |
| CN114431333A (en) * | 2021-12-24 | 2022-05-06 | 青岛尚德生物技术有限公司 | Phaffia rhodozyma liquid composite preparation and preparation method and application thereof |
| CN114431333B (en) * | 2021-12-24 | 2024-04-02 | 青岛尚德生物技术有限公司 | Phaffia rhodozyma liquid compound preparation and preparation method and application thereof |
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