CN105445083A - Centrifugation-free whole-blood processing module - Google Patents
Centrifugation-free whole-blood processing module Download PDFInfo
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- CN105445083A CN105445083A CN201510966838.0A CN201510966838A CN105445083A CN 105445083 A CN105445083 A CN 105445083A CN 201510966838 A CN201510966838 A CN 201510966838A CN 105445083 A CN105445083 A CN 105445083A
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- 239000008280 blood Substances 0.000 title claims abstract description 31
- 239000012528 membrane Substances 0.000 claims abstract description 98
- 239000003365 glass fiber Substances 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims abstract description 8
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 5
- 230000002522 swelling effect Effects 0.000 claims abstract description 3
- 210000004369 blood Anatomy 0.000 claims description 29
- 230000004888 barrier function Effects 0.000 claims description 6
- 238000010276 construction Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 abstract description 10
- 238000001179 sorption measurement Methods 0.000 abstract description 6
- 239000011148 porous material Substances 0.000 abstract description 4
- 238000005119 centrifugation Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 12
- 230000008569 process Effects 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 239000000427 antigen Substances 0.000 description 5
- 102000036639 antigens Human genes 0.000 description 5
- 108091007433 antigens Proteins 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000000945 filler Substances 0.000 description 3
- 230000028993 immune response Effects 0.000 description 3
- 241000228212 Aspergillus Species 0.000 description 2
- 241000222122 Candida albicans Species 0.000 description 2
- 241001337994 Cryptococcus <scale insect> Species 0.000 description 2
- 206010017533 Fungal infection Diseases 0.000 description 2
- 208000031888 Mycoses Diseases 0.000 description 2
- 229940095731 candida albicans Drugs 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 210000002381 plasma Anatomy 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- OMDQUFIYNPYJFM-XKDAHURESA-N (2r,3r,4s,5r,6s)-2-(hydroxymethyl)-6-[[(2r,3s,4r,5s,6r)-4,5,6-trihydroxy-3-[(2s,3s,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]methoxy]oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)[C@H](O)[C@H](O)[C@H](O)O1 OMDQUFIYNPYJFM-XKDAHURESA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 229920000926 Galactomannan Polymers 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 206010021703 Indifference Diseases 0.000 description 1
- 229920000057 Mannan Polymers 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
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- 230000003247 decreasing effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000003018 immunosuppressive agent Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 238000004375 physisorption Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
A provided centrifugation-free whole-blood processing module successively comprises an upper-layer filter membrane, an adsorption layer and a lower-layer filter membrane from top to bottom; the upper-layer filter membrane is composed of glass fiber filter paper or a millipore membrane, the upper-layer filter membrane is formed by superposing multiple layers of the filter membranes, and the pore diameter of the upper-layer filter membrane is gradually reduced from top to bottom; the adsorption layer employs a hydrophobic material with no swelling effect; the lower-layer filter membrane is composed of glass fiber filter paper or a millipore membrane, and the pore diameter of the lower-layer filter membrane is smaller than the pore diameter of the upper-layer filter membrane; and the outer side of the adsorption layer is provided with an outer ring. Power is supplied for filtering by means of syringe pressure through manual pressure, high-cost centrifugation equipment and complex processing steps are not needed, and sample consumption also is reduced.
Description
Technical field
The invention belongs to whole blood process field, especially relate to one and exempt from centrifugal whole blood processing module.
Background technology
In recent years, along with antibiotic a large amount of abuse and immunodepressant widely use clinical, the incidence of disease and the case fatality rate of aggressive deep fungal infection are more and more higher.Wherein, aspergillus, cryptococcus and candida albicans have become several important pathomycete clinically gradually.The Exoantigen of Aspergillus, cryptococcus and candida albicans is respectively galactomannan antigen, capsular polysaccharide antigen and mannan antigen, can find in early days in infection from blood samples of patients.Therefore be purposes the most a kind of method during current fungal infection detects based on immune response detectable antigens, but the method has higher requirement to the removal of impurities of blood sample and purifying.
Need to carry out process in early stage to blood or body fluid based on immunoreactive vitro detection, remove unnecessary interfering material, just can make immune response more accurately and fast.Traditional whole blood process, need the blood sample that obtains after anticoagulant process, carry out centrifugal treating, other composition (blood platelet, plasma-albumin, globulin etc.) in haemocyte (red blood cell, leucocyte) and blood is precipitated, and obtains supernatant (blood plasma).When utilizing immune response to detect polysaccharide antigen, blood supernatant also needs further process, adds treating fluid, as EDTA etc., through heating the means such as centrifugal, removes the most of protein in blood plasma.Above blood processing means need to use the equipment such as hydro-extractor, are unfavorable for micro-fluidic chip integrated, considerably increase the time of detection and the consumption of sample.Needed for micro-fluidic chip, sample is less, can be carried out the process of blood, obtain the sample of required filtering protein by the method such as physical filtering and absorption.
The common method of physisorption protein has: molecular sieve, ion-exchange, drainage column and affinity chromatography etc.Can according to the difference of molecular weight of albumen size, the difference of the charging property of albumen, or with the specific binding of aglucon, reach separation and the absorption of protein.Because the design's fundamental purpose is filtering protein, therefore do not need specificity filtering, therefore adopt the method for hydrophobic material indifference opposite sex absorption.
Summary of the invention
In view of this, the present invention is intended to propose one and exempts from centrifugal whole blood processing module, and whole blood sample, without the need to carrying out centrifugal treating, decreases the time of detection, reduces the consumption of sample.
For achieving the above object, technical scheme of the present invention is achieved in that
One exempts from centrifugal whole blood processing module, comprise upper strata filter membrane successively from top to bottom, adsorbed layer and lower floor's filter membrane, described upper strata filter membrane is made up of glass fiber filter paper or microporous barrier, described upper strata filter membrane is formed by stacking by multilayer filter membrane, described upper strata filter sizes from top to bottom reduces gradually, the present invention adopts filter membrane sandwich to filter adsorption structure, the fundamental purpose of upper strata filter membrane is filtering haemocyte, described adsorbed layer is the hydrophobic material without swelling action, described lower floor filter membrane is made up of glass fiber filter paper or microporous barrier, the aperture of described lower floor filter membrane is less than the aperture of described upper strata filter membrane, preferably, described upper strata filter membrane and described lower floor filter membrane are circle, the outside of described adsorbed layer is provided with outer ring.
Further, described adsorbed layer is C18 dewatering filling, the aperture of described adsorbed layer is less than the aperture of described upper strata filter membrane, middle layer is hydrophobic adsorbent layer, filler is the dewatering filling of the unsoluble-unexpansive matter such as C18, carry out without adsorption selection protein, the material that loading can detect according to difference requires to prepare different size to protein abundance in serum after process.Because central filler material is in dry environment before use, be directly used in a large amount of wastes that filtration can cause sample, adsorption efficiency may be made to reduce simultaneously, therefore preferably soak with damping fluid or distilled water before the use.
Further, described outer ring is hollow cylinder, the external diameter of described outer ring is more than or equal to the diameter of described upper strata filter membrane and described lower floor filter membrane, the internal diameter of described outer ring is less than the diameter of described upper strata filter membrane and described lower floor filter membrane, the object of such setting is, the edge of described upper strata filter membrane and described lower floor filter membrane all can drop on described outer ring, and the effect of fixing described upper strata filter membrane, lower floor's filter membrane and described adsorbed layer can be played in described outer ring.
Further, the connection bonding with described outer ring of described upper strata filter membrane, the connection bonding with described outer ring of described lower floor filter membrane, the periphery of described upper strata filter membrane is bonding with the upper surface of described outer ring, the periphery connection bonding with the lower surface of described outer ring of described lower floor filter membrane, the center section of described upper strata filter membrane and described lower floor filter membrane can normally use.
Further, the number of plies of described upper strata filter membrane is 3-5 layer, and the aperture of described upper strata filter membrane is between 0.2 μm-10 μm, for ensureing filtering high-level efficiency and high flow rate, the filtering membrane gradient of decreasing pore size is adopted to stack, in order to avoid too much haemocyte filters simultaneously, blocking microporous, cause cutout phenomenon.
Further, be combined with each other in the mode that periphery is bonding between the filter membrane of described upper strata, time bonding, by described upper strata filter membrane periphery bonding, the center section of described upper strata filter membrane can normally use.
Further, the Main Function of described lower floor filter membrane is as carrying, the aperture of preferred described lower floor filter membrane is slightly less than middle layer filler diameter, preferred employing glass fiber material is to reduce the unnecessary waste of sample, the below of described lower floor filter membrane is provided with arc collection unit, described arc collection unit comprises the taper cambered surface that circumference is arranged and the collection mouth be positioned at below described cambered surface, described cambered surface raises up, described cambered surface efficiently can collect liquid, and instill desired zone, described collection mouth is positioned at the centre of described arc collection unit, the size of described collection mouth is mated with the exit size of commercially available syringe.
Further, the top of described upper strata filter membrane is provided with threaded portion, the maximum outside diameter of described threaded portion equals the external diameter of described outer ring, the centre of described threaded portion is hollow-core construction, described upper strata filter membrane is now positioned at the inside of described threaded portion, preferably, the diameter of described upper strata filter membrane equals the internal diameter of described threaded portion, now, gapless between described upper strata filter membrane and described threaded portion, under avoiding blood lateral flow, described threaded portion is set, on the one hand, the friction force with syringe inner chamber body can be increased, on the other hand, internal thread is provided with in syringe cavity commercially available at present, preferably, by the screw-internal thread fit of the external thread of described threaded portion and commercially available syringe, better strength of joint can be obtained, described threaded portion is fixedly connected with the top of described outer ring, connected mode is welding or bonding, also can be one-body molded.
During use, it should be noted that, the internal diameter specification of commercially available syringe cavity is different, pin for the purpose of the present invention, the external diameter of described outer ring is equal with the internal diameter of supporting syringe, the present invention is inserted in commercially available syringe, screw in one end of syringe outlet, pending blood is injected in syringe cavity, injection mode can be, but not limited to utilize dropper instill or use other syringes to inject, then, syringe piston is utilized to promote blood sample, blood sample is through described upper strata filter membrane, after adsorbed layer and lower floor's filter membrane, flow out from the outlet of syringe, enter micro-fluidic sample inlet district to detect.
Relative to prior art, the present invention has following advantage: manual pressure, providing power, neither needing the centrifugation apparatus of high cost and the treatment step of complexity, also can reduce the consumption of sample by syringe pressure for filtering.
Accompanying drawing explanation
The accompanying drawing forming a part of the present invention is used to provide a further understanding of the present invention, and schematic description and description of the present invention, for explaining the present invention, does not form inappropriate limitation of the present invention.In the accompanying drawings:
Fig. 1 is the main apparent direction structural representation of the embodiment of the present invention 1;
Fig. 2 is the main apparent direction structural representation of the embodiment of the present invention 2;
Fig. 3 is the embodiment of the present invention 3 stereo directional decomposition texture schematic diagram;
Fig. 4-5 is the main apparent direction structural representation of the embodiment of the present invention 3.
Description of reference numerals:
1-upper strata filter membrane; 2-adsorbed layer; 3-lower floor filter membrane; 4-outer ring; 5-arc collection unit; 51-cambered surface; 52-collects mouth; 6-threaded portion.
Embodiment
It should be noted that, when not conflicting, the embodiment in the present invention and the feature in embodiment can combine mutually.
In describing the invention, it will be appreciated that, term " " center ", " longitudinal direction ", " transverse direction ", " on ", D score, " front ", " afterwards ", " left side ", " right side ", " vertically ", " level ", " top ", " end ", " interior ", orientation or the position relationship of the instruction such as " outward " are based on orientation shown in the drawings or position relationship, only the present invention for convenience of description and simplified characterization, instead of indicate or imply that the device of indication or element must have specific orientation, with specific azimuth configuration and operation, therefore limitation of the present invention can not be interpreted as.In addition, term " first ", " second " etc. only for describing object, and can not be interpreted as instruction or hint relative importance or imply the quantity indicating indicated technical characteristic.Thus, one or more these features can be expressed or impliedly be comprised to the feature being limited with " first ", " second " etc.In describing the invention, except as otherwise noted, the implication of " multiple " is two or more.
In describing the invention, it should be noted that, unless otherwise clearly defined and limited, term " installation ", " being connected ", " connection " should be interpreted broadly, and such as, can be fixedly connected with, also can be removably connect, or connect integratedly; Can be mechanical connection, also can be electrical connection; Can be directly be connected, also indirectly can be connected by intermediary, can be the connection of two element internals.For the ordinary skill in the art, above-mentioned term concrete meaning in the present invention can be understood by concrete condition.
Below with reference to the accompanying drawings and describe the present invention in detail in conjunction with the embodiments.
Embodiment 1
Exempt from centrifugal whole blood processing module, comprise upper strata filter membrane 1 successively from top to bottom, adsorbed layer 2 and lower floor's filter membrane 3, described upper strata filter membrane 1 is made up of glass fiber filter paper or microporous barrier, the number of plies of described upper strata filter membrane 1 is 3-5 layer, be combined with each other in the mode that periphery is bonding between described upper strata filter membrane 1, the aperture of described upper strata filter membrane 1 is between 0.2 μm-10 μm, filter membrane 1 aperture, described upper strata from top to bottom reduces gradually, described adsorbed layer 2 is C18 dewatering filling, the aperture of described adsorbed layer 2 is less than the aperture of described upper strata filter membrane 1, described lower floor filter membrane 3 is made up of glass fiber filter paper or microporous barrier, the aperture of described lower floor filter membrane 3 is less than the aperture of described upper strata filter membrane 1, described upper strata filter membrane 1 and described lower floor filter membrane 3 are circle, the outside of described adsorbed layer 2 is provided with outer ring 4, described outer ring 4 is hollow cylinder, the external diameter of described outer ring 4 is more than or equal to the diameter of described upper strata filter membrane 1 and described lower floor filter membrane 3, the internal diameter of described outer ring 4 is less than the diameter of described upper strata filter membrane 1 and described lower floor filter membrane 3, described upper strata filter membrane 1 and the bonding connection in described outer ring 4, described lower floor filter membrane 3 and the bonding connection in described outer ring 4.
Embodiment 2
Embodiment 2 is substantially identical with the structure of embodiment 1, embodiment 2 is with the difference of embodiment 1: the below of described lower floor filter membrane 3 is provided with arc collection unit 5, described arc collection unit 5 comprises the taper cambered surface 51 that circumference is arranged and the collection mouth 52 be positioned at below described cambered surface 51, and described cambered surface 51 raises up.
Embodiment 3
Embodiment 3 is substantially identical with the structure of embodiment 1, embodiment 2 is with the difference of embodiment 1: the top of described upper strata filter membrane 1 is provided with threaded portion 6, the maximum outside diameter of described threaded portion 6 equals the external diameter of described outer ring 4, and the centre of described threaded portion 6 is hollow-core construction.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (9)
1. exempt from centrifugal whole blood processing module, it is characterized in that: comprise upper strata filter membrane, adsorbed layer and lower floor's filter membrane from top to bottom successively, described upper strata filter membrane is made up of glass fiber filter paper or microporous barrier, described upper strata filter membrane is formed by stacking by multilayer filter membrane, described upper strata filter sizes from top to bottom reduces gradually, described adsorbed layer is the hydrophobic material without swelling action, described lower floor filter membrane is made up of glass fiber filter paper or microporous barrier, the aperture of described lower floor filter membrane is less than the aperture of described upper strata filter membrane, and the outside of described adsorbed layer is provided with outer ring.
2. according to claim 1ly exempt from centrifugal whole blood processing module, it is characterized in that: described adsorbed layer is C18 dewatering filling, the aperture of described adsorbed layer is less than the aperture of described upper strata filter membrane.
3. according to claim 1ly exempt from centrifugal whole blood processing module, it is characterized in that: described upper strata filter membrane and described lower floor filter membrane are circle.
4. according to claim 3ly exempt from centrifugal whole blood processing module, it is characterized in that: described outer ring is hollow cylinder, the external diameter of described outer ring is more than or equal to the diameter of described upper strata filter membrane and described lower floor filter membrane, and the internal diameter of described outer ring is less than the diameter of described upper strata filter membrane and described lower floor filter membrane.
5. according to claim 1ly exempt from centrifugal whole blood processing module, it is characterized in that: the connection bonding with described outer ring of described upper strata filter membrane, the connection bonding with described outer ring of described lower floor filter membrane.
6. according to claim 1ly exempt from centrifugal whole blood processing module, it is characterized in that: the number of plies of described upper strata filter membrane is 3-5 layer, and the aperture of described upper strata filter membrane is between 0.2 μm-10 μm.
7. according to claim 1ly exempt from centrifugal whole blood processing module, it is characterized in that: be combined with each other in the mode that periphery is bonding between the filter membrane of described upper strata.
8. according to claim 1ly exempt from centrifugal whole blood processing module, it is characterized in that: the below of described lower floor filter membrane is provided with arc collection unit, described arc collection unit comprises the taper cambered surface that circumference is arranged and the collection mouth be positioned at below described cambered surface, and described cambered surface raises up.
9. according to claim 1ly exempt from centrifugal whole blood processing module, it is characterized in that: the top of described upper strata filter membrane is provided with threaded portion, the maximum outside diameter of described threaded portion equals the external diameter of described outer ring, and the centre of described threaded portion is hollow-core construction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510966838.0A CN105445083B (en) | 2015-12-21 | 2015-12-21 | Exempt to centrifuge whole blood processing module |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510966838.0A CN105445083B (en) | 2015-12-21 | 2015-12-21 | Exempt to centrifuge whole blood processing module |
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| Publication Number | Publication Date |
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| CN105445083A true CN105445083A (en) | 2016-03-30 |
| CN105445083B CN105445083B (en) | 2018-09-11 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106840828A (en) * | 2017-03-29 | 2017-06-13 | 天津中新科炬生物制药股份有限公司 | The method and separator of quick separating blood plasma in a kind of micro whole blood |
| CN107638810A (en) * | 2016-07-20 | 2018-01-30 | 中国石油天然气股份有限公司 | Filter membrane assembly and filter device with same |
| CN108362543A (en) * | 2018-02-08 | 2018-08-03 | 上海蓝怡科技股份有限公司 | A kind of serum separator and the method using its progress serum separation |
| CN109925884A (en) * | 2019-04-27 | 2019-06-25 | 南京岚煜生物科技有限公司 | A kind of method of Whole Blood Filtration and filter membrane structure for Whole Blood Filtration |
| CN115407078A (en) * | 2022-11-01 | 2022-11-29 | 丹娜(天津)生物科技股份有限公司 | Analyzer with sample preprocessing function and sample preprocessing method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1142346A (en) * | 1995-08-09 | 1997-02-12 | 中国科学院大连化学物理研究所 | Blood plasma separator for clinic laboratory analysis on blood constituents |
| CN1248171A (en) * | 1996-11-08 | 2000-03-22 | 帕尔公司 | Method for purifying blood plasma and apparatus suitable therefor |
| CN1684727A (en) * | 2002-09-12 | 2005-10-19 | 旭化成医疗有限公司 | Plasma purifying membrane and plasma purifying system |
| CN1714292A (en) * | 2002-11-19 | 2005-12-28 | 积水化学工业株式会社 | Plasma or serum separation membrane and filter including plasma or serum separation membrane |
| CN104519976A (en) * | 2012-08-09 | 2015-04-15 | 霍夫曼-拉罗奇有限公司 | Multi-part device for extracting plasma from blood |
| CN104548232A (en) * | 2013-10-15 | 2015-04-29 | 金鸿 | Device and method of extracting high-concentration plasma from whole blood |
| CN205301048U (en) * | 2015-12-21 | 2016-06-08 | 丹娜（天津）生物科技有限公司 | Centrifugal whole blood processing module exempts from |
-
2015
- 2015-12-21 CN CN201510966838.0A patent/CN105445083B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1142346A (en) * | 1995-08-09 | 1997-02-12 | 中国科学院大连化学物理研究所 | Blood plasma separator for clinic laboratory analysis on blood constituents |
| CN1248171A (en) * | 1996-11-08 | 2000-03-22 | 帕尔公司 | Method for purifying blood plasma and apparatus suitable therefor |
| CN1684727A (en) * | 2002-09-12 | 2005-10-19 | 旭化成医疗有限公司 | Plasma purifying membrane and plasma purifying system |
| CN1714292A (en) * | 2002-11-19 | 2005-12-28 | 积水化学工业株式会社 | Plasma or serum separation membrane and filter including plasma or serum separation membrane |
| CN104519976A (en) * | 2012-08-09 | 2015-04-15 | 霍夫曼-拉罗奇有限公司 | Multi-part device for extracting plasma from blood |
| CN104548232A (en) * | 2013-10-15 | 2015-04-29 | 金鸿 | Device and method of extracting high-concentration plasma from whole blood |
| CN205301048U (en) * | 2015-12-21 | 2016-06-08 | 丹娜（天津）生物科技有限公司 | Centrifugal whole blood processing module exempts from |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107638810A (en) * | 2016-07-20 | 2018-01-30 | 中国石油天然气股份有限公司 | Filter membrane assembly and filter device with same |
| CN106840828A (en) * | 2017-03-29 | 2017-06-13 | 天津中新科炬生物制药股份有限公司 | The method and separator of quick separating blood plasma in a kind of micro whole blood |
| CN106840828B (en) * | 2017-03-29 | 2020-02-14 | 天津中新科炬生物制药股份有限公司 | Method and device for quickly separating plasma from trace whole blood |
| CN108362543A (en) * | 2018-02-08 | 2018-08-03 | 上海蓝怡科技股份有限公司 | A kind of serum separator and the method using its progress serum separation |
| CN109925884A (en) * | 2019-04-27 | 2019-06-25 | 南京岚煜生物科技有限公司 | A kind of method of Whole Blood Filtration and filter membrane structure for Whole Blood Filtration |
| WO2020220157A1 (en) * | 2019-04-27 | 2020-11-05 | 南京岚煜生物科技有限公司 | Whole blood filtering method and filter membrane structure for whole blood filtering |
| US12128365B2 (en) | 2019-04-27 | 2024-10-29 | Lansion Biotechnology Co., Ltd | Method for whole blood filtration and filter membrane structure for whole blood filtration |
| CN115407078A (en) * | 2022-11-01 | 2022-11-29 | 丹娜(天津)生物科技股份有限公司 | Analyzer with sample preprocessing function and sample preprocessing method |
| CN115407078B (en) * | 2022-11-01 | 2023-01-31 | 丹娜(天津)生物科技股份有限公司 | Analyzer with sample preprocessing function and sample preprocessing method |
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