[go: up one dir, main page]

CN105445073B - A kind of fast protein dyeing liquor - Google Patents

A kind of fast protein dyeing liquor Download PDF

Info

Publication number
CN105445073B
CN105445073B CN201511006847.1A CN201511006847A CN105445073B CN 105445073 B CN105445073 B CN 105445073B CN 201511006847 A CN201511006847 A CN 201511006847A CN 105445073 B CN105445073 B CN 105445073B
Authority
CN
China
Prior art keywords
fast protein
dyeing
need
dyeing liquor
sodium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201511006847.1A
Other languages
Chinese (zh)
Other versions
CN105445073A (en
Inventor
孔宁
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qingdao Aidi Biotechnology Co Ltd
Original Assignee
Qingdao Aidi Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qingdao Aidi Biotechnology Co Ltd filed Critical Qingdao Aidi Biotechnology Co Ltd
Priority to CN201511006847.1A priority Critical patent/CN105445073B/en
Publication of CN105445073A publication Critical patent/CN105445073A/en
Application granted granted Critical
Publication of CN105445073B publication Critical patent/CN105445073B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention discloses a kind of fast protein dyeing liquors, it is improved in that including following ingredient：20 grams of solute, including Coomassie brilliant blue R 250 0.4 0.6%, sodium iodide 40 45%, sodium phosphate 15 20%, sodium acetate 15 20% and metaphosphoric acid 20 25%；Distilled water 95ml, absolute ethyl alcohol 5ml, dense HCl 350ul.Fast protein dyeing liquor disclosed in this invention need to only use water when decoloration, need not contact methanol and acetic acid again.Also, dyeing only needs half an hour, need not substantially decolourize, protein band can be clearly presented.The time is greatly saved.

Description

A kind of fast protein dyeing liquor
Technical field
The present invention relates to a kind of fast protein dyeing liquors.
Background technology
When laboratory experiment personnel carry out protein staining, traditional dyeing liquor is usually used.Main component is coomassie Brilliant blue R-250, methanol and acetic acid.First, methanol and all irritant smell of acetic acid, are easy to keep operator under the weather, Secondly, it after the completion of dyeing, needs to be decolourized with destainer, the ingredient of destainer is methanol and acetic acid, also irritant gas Taste.Finally, staining procedure generally requires 1 hour or more time, and decoloration is also required to 2 hours or more time.
Invention content
The technical problems to be solved by the invention are just to provide a kind of fast protein dyeing liquor.
In order to solve the above-mentioned technical problem, the present invention adopts the following technical scheme that：
A kind of fast protein dyeing liquor, it is improved in that including following ingredient：20 grams of solute, including coomassie are bright Blue R-250 0.4-0.6%, sodium iodide 40-45%, sodium phosphate 15-20%, sodium acetate 15-20% and metaphosphoric acid 20-25%；Distilled water 95ml, absolute ethyl alcohol 5ml, dense HCl 350ul.
The beneficial effects of the invention are as follows：
Fast protein dyeing liquor disclosed in this invention need to only use water when decoloration, need not contact methanol and acetic acid again.And And dyeing only needs half an hour, need not substantially decolourize, protein band can be clearly presented.The time is greatly saved.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to Limit the present invention.
Embodiment 1, present embodiment discloses a kind of fast protein dyeing liquors, including following ingredient：20 grams of solute, including examine Mas bright blue R-250 0.4-0.6%, sodium iodide 40-45%, sodium phosphate 15-20%, sodium acetate 15-20% and metaphosphoric acid 20-25%； Solvent includes distilled water 95ml, absolute ethyl alcohol 5ml, dense HCl 350ul.
Film to be contaminated is put into dyeing liquor, microwave heating 10-15 seconds to fluid temperature is 50-60 DEG C, in dyeing shaking table It is upper dyeing 30 minutes, after film is taken out, with clear water rinse 10 seconds or so, protein band can be presented.

Claims (1)

1. a kind of fast protein dyeing liquor, which is characterized in that including following ingredient：20 grams of solute, including coomassie brilliant blue R_250 0.4-0.6%, sodium iodide 40-45%, sodium phosphate 15-20%, sodium acetate 15-20% and metaphosphoric acid 20-25%；Distilled water 95ml, it is anhydrous 350 μ l of ethyl alcohol 5ml, dense HCl.
CN201511006847.1A 2015-09-14 2015-12-30 A kind of fast protein dyeing liquor Expired - Fee Related CN105445073B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201511006847.1A CN105445073B (en) 2015-09-14 2015-12-30 A kind of fast protein dyeing liquor

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201510578831 2015-09-14
CN2015105788311 2015-09-14
CN201511006847.1A CN105445073B (en) 2015-09-14 2015-12-30 A kind of fast protein dyeing liquor

Publications (2)

Publication Number Publication Date
CN105445073A CN105445073A (en) 2016-03-30
CN105445073B true CN105445073B (en) 2018-09-25

Family

ID=55555532

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201511006847.1A Expired - Fee Related CN105445073B (en) 2015-09-14 2015-12-30 A kind of fast protein dyeing liquor

Country Status (1)

Country Link
CN (1) CN105445073B (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1904578A (en) * 2006-08-02 2007-01-31 中国科学院植物研究所 Kaomas brilliant blue dyeing method and its special gel fixation liquid and dyeing agent

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6319720B1 (en) * 1998-10-13 2001-11-20 Ewald M. Wondrak Process for fast visualization of protein

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1904578A (en) * 2006-08-02 2007-01-31 中国科学院植物研究所 Kaomas brilliant blue dyeing method and its special gel fixation liquid and dyeing agent

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
A New Staining Technique for Proteins in Polyacrylamide Gels Using Coomassie Brilliant Blue G250;ROBERT W.BLAKESLEY等;《ANALYTICAL BIOCHEMISTRY》;19771231;第580页第1-4段 *
Exposure of Platelet Fibrinogen Receptors by ADP and Epinephrine;JOEL.S等;《J.Clin.Invest》;19791231;第1393页右栏最后1段-第1394页右栏第1段 *
Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant blue G-250 and R-250;Volker Neuhoff等;《Electrophoresis》;19881231;第9卷;第255-262页 *
血清蛋白醋纤维薄膜电泳易出现的问题及对策;孙设宗等;《郧阳医学院学报》;20050630;第24卷(第3期);第183-184页 *

Also Published As

Publication number Publication date
CN105445073A (en) 2016-03-30

Similar Documents

Publication Publication Date Title
MX2024010252A (en) Processes for reducing lactic acid in a biofuel fermentation system
MX357743B (en) Shaping keratin fibres using 2-hydroxypropane-1,2,3-tricarboxylic acid and/or 1,2,3,4-butanetetracarboxylic acid.
WO2014176487A3 (en) Chelated nanoceria for the treatment of oxidative stress
CN105445073B (en) A kind of fast protein dyeing liquor
MX2021006808A (en) Dephosphorylation enzyme of new psicose-6-phosphoric acid, composition for producing psicose comprising same, and method for preparing psicose using same.
WO2017093866A3 (en) An improved process for the preparation of indigo carmine
CN103834525A (en) Red yeast preparation method
CN104294610A (en) Waterproof finish method for clothes
GB604947A (en) Method of improving the adsorbent properties of alumina
MX2015015317A (en) Low 4-methylimidazole caramel color class iv production.
GB459820A (en) Process for stabilising cellulose esters
LT2013052A (en) Process for producing bog oak
Liu et al. Erratum: PKM2 methylation by CARM1 activates aerobic glycolysis to promote tumorigenesis
CN102150567B (en) flower dyeing process
MX2019010207A (en) Process for recovering an esterified cellulose ether from a reaction product mixture.
CN103696283A (en) Dyeing process of acid dye
PL409976A1 (en) Method for obtaining food additive in order to stabilize the texture or concentration, preferably of the apple pomace and the additive obtained by this method
CN110800910A (en) A kind of microwave drying method of honeysuckle
CN104626870A (en) Fresh flower bleaching technology
CN106581768A (en) Preparing method of scaffold for tissue engineering used for myocardium repair
CN204694047U (en) A kind of dried casing drying unit
GB466356A (en) Improvements relating to the production of pectin
EP4495169A3 (en) Cellulose molded body and hydrogel, and method for producing the same
CN108151446A (en) A kind of fence board rapid production process
GB482826A (en) Manufacture of extracts of spleen or liver

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20180925

Termination date: 20191230

CF01 Termination of patent right due to non-payment of annual fee