CN105352923B - A kind of quick wide visual field volume holographic fluorescence microimaging systems - Google Patents

Abstract

The invention relates to a fast wide-field volume holographic fluorescent microscopic imaging system, which is characterized in that it includes a laser light source, a dichroic beam splitter, a microscopic objective lens, a MEMS microreflector array device, a volume holographic grating device, and an imaging lens and the image detector array; the laser light source emits the illumination light wave to the dichroic beam splitter; the dichroic beam splitter irradiates the illumination light wave through the microscopic objective lens to the imaging target, and the fluorescence emitted by the imaging target returns to the dichroic beam splitter through the microscopic objective lens, The microscopic objective lens projects the fluorescence to the MEMS micro-mirror array device; the MEMS micro-mirror array device encodes the angle of the light waves at different positions according to the central wavelength of the imaging spectrum and the Bragg characteristic parameters of the volume holographic grating device, and then controls the angle of the imaging beam. The deflection direction, the deflected imaging beam is incident on the volume holographic grating device at a matching angle; the volume holographic grating device diffracts the incident light encoded by the spatial angle, and the diffracted light is decoded by the MEMS micro-mirror decoding array device and then passes through the imaging lens Imaging onto the image detector array.

Description

A fast wide-field volume holographic fluorescence microscopy imaging system

technical field

The invention relates to the technical field of spatial spectrum microscopic imaging, in particular to a fast wide-field volume holographic fluorescent microscopic imaging system.

Background technique

The spectral microscopic imaging system based on volume holographic grating is a new microscopic imaging technology that has attracted much attention in recent years. It has a very broad application in the fields of shallow tumor detection, disease diagnosis, food and drug supervision, biological inspection and quarantine, and material science Application prospect. The volume holographic microspectral imaging system uses the Bragg selection characteristics of the volume holographic grating device to realize microscopic imaging with high spectral resolution. The volume holographic grating device is made by using a photorefractive material with a certain thickness to record the coherent pattern of two beams of coherent light in the recording material. The recorded holographic pattern is a three-dimensional phase grating with a thickness. This three-dimensional phase grating It has special diffraction characteristics, fine spectral angle selection characteristics, volume multiplexing characteristics, and has a very wide spectral adjustment range, which can realize continuous adjustment in a wide spectral range. When a certain wavelength of light is used to irradiate biological tissue, the surface of the biological tissue will scatter and absorb the incident light. When the scattered light passes through the microscope objective lens and enters the surface of the volume holographic grating located in the focal plane of the image square, the volume holographic grating The Bragg selection characteristic makes only the incident light wave matching the incident angle pass through with high diffraction efficiency, so that the image of the narrow-band spectrum of the tissue can be obtained, and the interference of background light can be avoided.

The volume holographic optical imaging system can realize imaging of a narrow field of view under a narrow-band light source and obtain spectral information of the corresponding area, and use volume multiplexing volume holographic gratings to obtain three-dimensional spatial tomographic images of translucent tissues and perform contour imaging of non-transparent structures. Due to the degeneracy of the volume holographic grating, when the surface of the object reflects or emits light with a wide spectrum, the image appearing on the rear focal plane of the imaging lens is an image containing multiple colors of light. Due to the Bragg selection characteristics of the volume holographic grating, under the narrow-band light illumination condition, the pattern obtained on the image sensor is a narrow band. If you want to obtain a wide-field image of a single color light, you need to use a horizontal scanning method, but this solution increases The complexity of the system reduces the imaging efficiency, and it cannot realize the continuous and fast switching of multiple monochromatic spectra with a large field of view.

Contents of the invention

In view of the above problems, the object of the present invention is to provide a wide-field volume holographic fluorescence microscopic imaging system that can be used for imaging wide-spectrum imaging targets and can realize fast switching of single spectrum.

In order to achieve the above object, the present invention adopts the following technical solutions: a fast wide-field volume holographic fluorescence microscopic imaging system, characterized in that it includes a laser light source, a dichroic beam splitter, a microscopic objective lens, a MEMS A micromirror array device, an integrated holographic grating device, an imaging lens, and an image detector array, wherein the MEMS micromirror array device is located at the image plane of the microscopic objective lens and is arranged on the volume holographic grating device The incident end; the laser light source is used to provide an illumination condition with uniform intensity for an imaging target, and the laser light source emits an illumination light wave to the dichroic beam splitter; the dichroic beam splitter turns the illumination light wave to be perpendicular to Its incident direction and irradiates the imaging target through the microscopic objective lens, and the fluorescence emitted by the imaging target returns to the dichroic beam splitter through the microscopic objective lens, and the dichroic beam splitter filters out the imaging target The target emits the illumination light wave contained in the fluorescence, and the fluorescence emitted by the imaging target is projected to the MEMS micro-mirror array device through the microscope objective; the MEMS micro-mirror array device is based on the central wavelength of the imaging spectrum and the The Bragg characteristic parameters of the volume holographic grating device encode the angles of the light waves at different positions to control the deflection direction of the imaging beam, and the deflected imaging beam is incident on the volume holographic grating device at a matching angle; the volume holographic grating device is The incident light encoded by the spatial angle is diffracted, and the diffracted light is imaged to the image detector array through the imaging lens.

Further, the volume holographic fluorescence microscopic imaging system also includes a MEMS micromirror decoding array device for spatial angle decoding, and the MEMS micromirror decoding array device is located at the focal plane of the object space of the imaging lens And it is arranged at the diffraction output end of the volume holographic grating device; the volume holographic grating device diffracts the incident light after spatial angle encoding and transmits the diffracted light to the MEMS micromirror decoding array device, and the MEMS The micro-mirror decoding array device controls the driving force of each column of micro-mirrors for spatial decoding according to the deflection angle of each column of micro-mirrors on the MEMS micro-mirror array device for space angle encoding, and realizes a completely symmetrical angle deflection to decode the coded diffracted light, and the MEMS micro-mirror decoding array device images the decoded diffracted light to the image detector array through the imaging lens.

Further, the volume holographic grating device adopts a single-channel reflective volume holographic grating device, a single-channel transmissive volume holographic grating device, a multi-channel wavelength multiplexing reflective volume holographic grating device or a multiple wavelength multiplexing transmissive volume holographic grating device .

Further, the spatial angle encoding and decoding methods of the MEMS micromirror array device and the MEMS micromirror decoding array device specifically include the following two forms according to the characteristics of the imaging target and the imaging spectrum requirements: ① When the imaging target needs to be acquired According to the matching condition and imaging wavelength of the volume holographic grating device, the deflection angle of each row of micro-mirrors in the MEMS micro-mirror array device is controlled, so that the imaging light wave can be incident on the set at a Bragg-matched angle. The volume holographic grating device, by controlling the MEMS micro-mirror decoding array device to decode the selected diffracted single-spectrum light, directly obtains a wide-field single-spectrum image on the image detector; ② When it is necessary to continuously acquire the When imaging multiple images of different wavelengths that continuously change in a short period of time, according to the matching conditions of the volume holographic grating device and the selected wavelength parameters of different imaging spectra, the microreflection of each column of the MEMS micromirror array device is controlled. The deflection angle of the mirror is realized by encoding the deflection angle of the MEMS micro-mirror array device to quickly switch the imaging spectrum, and by controlling the MEMS micro-mirror decoding array device to decode the selected diffracted single-spectrum light, directly Sequentially obtain single-spectrum images corresponding to different spectral bands of the wide field of view on the image detector.

Due to the adoption of the above technical scheme, the present invention has the following advantages: 1. The present invention adopts MEMS micro-mirror array devices, MEMS micro-mirror decoding array devices and combines volume holographic grating devices to construct a spatial angle encoding and decoding module to spatially perform imaging regions. Angle encoding and decoding, according to the characteristics of the imaging target and the requirements of the imaging spectrum, the spatial angle encoding and decoding method is designed to realize single-spectrum imaging and multi-spectral imaging. The spectral switching speed of the spectral fluorescence microscope system expands the imaging field of view; compared with the existing spectral imaging system, it can not only easily realize wide-field single-spectrum imaging, but also realize fast switching of multiple monochromatic imaging spectra spectral imaging. 2. When the imaging target surface reflects or emits light with a wide spectrum, the image appearing on the rear focal plane of the imaging lens is an image containing multiple colors of light. The present invention quickly adjusts and switches MEMS micromirror array devices through spatial angle coding The inclination angle makes the corresponding wavelength in the field of view satisfy the Bragg condition of the incident volume holographic grating, and the image of a single color light in the wide imaging field of view can be directly obtained without using a filter device, which improves the imaging between different spectra Switch the speed to realize multiple continuous spectral imaging of wide field of view. 3. When the present invention is imaging an imaging target that emits fluorescence, multiple continuous spectral images of the imaging target can be obtained without the aid of an optical filter device, and it can quickly observe different spectral images of living tissues at different times in real time. Switch the inclination angle of each column of micro-mirrors in the MEMS micro-mirror array device, change the spatial angle encoding of the reflected light, and quickly realize multiple continuous spectral imaging, and the single-spectrum spectral width of the imaging can pass through the MEMS micro-mirror array according to actual needs The number of columns of coded micromirrors with the same deflection angle is adjusted. 4. The present invention uses a MEMS micro-mirror decoding array device to realize the decoding function. The MEMS micro-mirror decoding array device is located at the diffraction output end of the volume holographic grating device, and performs symmetrical spatial angle decoding on the image encoded by the spatial angle, which can directly restore Spatial information of incident diffracted light, and enables direct acquisition of wide-field single-spectrum images on image detector arrays. The invention has the advantages of simple structure, convenient use and high imaging efficiency, and can be widely used in fluorescence microscopic imaging of transmission and reflection imaging structures.

Description of drawings

Fig. 1 is a schematic structural diagram of the fast multi-spectral wide-field volume holographic fluorescence microscopic imaging system of the present invention, wherein, (a) the volume holographic grating device adopts a transmissive type, and (b) the volume holographic grating device adopts a reflective type;

Fig. 2 is a schematic diagram of the working principle of the spatial angle encoding and decoding module using a transmissive volume holographic grating in the present invention, wherein (a) is a schematic diagram of the single-wavelength working principle of a transmissive volume holographic grating, (b) a transmissive format recorded in a wavelength multiplexing manner Schematic diagram of multi-wavelength working principle of volume holographic grating, (c) schematic diagram of optical path principle of transmissive volume wavelength multiplexing holographic grating in spatial angle encoding and decoding;

Fig. 3 is a schematic diagram of the multi-wavelength working principle of the spatial angle encoding and decoding module using a reflective volume holographic grating in the present invention, wherein (a) is a schematic diagram of the single-wavelength working principle of a reflective volume holographic grating, and (b) is recorded in a wavelength multiplexing manner (c) is a schematic diagram of the optical path principle of the reflective multi-wavelength multiplexing volume holographic grating in the spatial angle encoding and decoding;

Fig. 4 is the structural representation of the MEMS micromirror array device that the present invention adopts, and wherein, (a) is the overall structural representation of MEMS microreflector array device, (b) is a single microreflector in the MEMS micromirror array device (c) is a schematic diagram of the working principle of the MEMS micromirror array device;

Fig. 5 is a schematic diagram of the principle of single-spectrum spatial angle encoding and decoding of the present invention;

Fig. 6 is a schematic diagram of the switching principle of spatial angle encoding when switching between spectra according to the present invention.

Detailed ways

The present invention will be described in detail below in conjunction with the accompanying drawings. However, it should be understood that the accompanying drawings are provided only for better understanding of the present invention, and they should not be construed as limiting the present invention.

As shown in Figure 1, the fast wide field of view volume holographic fluorescent microscopic imaging system of the present invention includes a laser light source 1, a dichroic beam splitter 2, a microscopic objective lens 4, an integrated holographic space angle encoding and decoding module 5, a Imaging lens 6 and an image detector array 7, wherein the volume holographic space angle codec module 5 includes a space angle encoder 51 composed of a MEMS micro-mirror array device, an integrated holographic grating device 52 and a MEMS micro-mirror decoding array The spatial angle decoder 53 (in the description of the present invention, it should be understood that the term "MEMS micro-mirror array device" and "MEMS micro-mirror array device" is only used for the purpose of description, and both The structures are identical), the MEMS micro-mirror array device 51 and the MEMS micro-mirror decoding array device 53 are respectively located at the incident end and the diffraction output end of the volume holographic grating device 52, and the MEMS micro-mirror array device 51 is located at the end of the microscopic objective lens 4 As for the image plane, the MEMS micromirror decoding array device 53 is located at the focal plane of the object space of the imaging lens 6 .

The laser light source 1 is used to provide uniform illumination conditions for the imaging target 3. The laser light source 1 emits the illumination light wave to the dichroic beam splitter 2; the dichroic beam splitter 2 turns the illumination light wave perpendicular to its incident direction and passes through the The objective lens 4 irradiates the imaging target 3, and the fluorescence emitted by the imaging target 3 returns to the dichroic beam splitter 2 through the microscopic objective lens 4, and the dichroic beam splitter 2 is used to filter out the illumination light wave contained in the fluorescence emitted by the imaging target 3, and the imaging target 3. The emitted fluorescence is projected onto the MEMS micro-mirror array device 51 through the microscopic objective lens 4, and the MEMS micro-mirror array device 51 calculates the reflection angle when the spectral width of the imaging spectrum and the central wavelength are matched with the bulk holographic grating device 52 according to the Bragg conditions, and obtains The spatial angle encoding of each column of micromirrors, and the driving force of each column of micromirrors is controlled according to the spatial angle encoding, and then the deflection direction of each column of micromirrors is controlled, so that the deflected imaging beam is incident on the The volume holographic grating device 52; the volume holographic grating device 52 diffracts the incident light encoded by the spatial angle and transmits the diffracted light to the MEMS micro-mirror decoding array device 53, and the MEMS micro-mirror decoding array device 53 is used according to the spatial angle The deflection angle of each column of micromirrors on the coded MEMS micromirror array device is used to control the driving force of each column of micromirrors for spatial decoding, to achieve completely symmetrical angle deflection, and then decode the encoded diffracted light The MEMS micromirror decoding array device 53 images the decoded diffracted light to the image detector array 7 through the imaging lens 6 .

In a preferred embodiment, as shown in FIG. 2 and FIG. 3 , the volume holographic grating device 52 may be a single-path or wavelength multiplexed reflective volume holographic grating device or a transmissive volume holographic grating device.

In a preferred embodiment, as shown in Figure 4 (a), MEMS micro-mirror array device 51 and MEMS micro-mirror decoding array device 53 are identical in structure, and the embodiment of the present invention only has MEMS micro-mirror array device 51 The structure is described, the MEMS micro-mirror array device 51 is an array formed by tens of thousands of micro-mirrors arranged neatly, and the shape of each micro-mirror can be a rectangle or a rhombus. The MEMS micro-mirror array device 51 includes a micro-mirror array, a driving circuit of the micro-mirror array and a corresponding microcontroller, the microcontroller is used to control the spatial angle encoding state of the micro-mirror array, and the driving circuit is used to control the micro-mirror array Array switch and micromirror array action. As shown in Figure 4 (b), each MEMS micro-mirror comprises a micro-mirror surface 513, a drive mechanism 512 and a support substrate 511, and the micro-mirror surface 513 is connected on the support substrate 511 by the drive mechanism 512, and the micro-mirror surface Driven by the bottom driving mechanism 512, the 513 can be tilted at any angle in a two-dimensional plane so as to control the reflection direction of light incident on the mirror surface. As shown in Figure 4(c), the basic principle of the MEMS micromirror array device to realize spatial angle encoding is: the microcontroller calculates the deflection angle parameters of each row of micromirrors according to the imaging spectrum parameters and volume holographic grating device parameters and Send it to the drive circuit, and the drive circuit controls the deflection angle of each micro-mirror to the drive mechanism according to the deflection angle parameters of each column of micro-mirrors, that is, by addressing the landing electrodes of the relevant i-th column of micro-mirrors , the free end of the driving mechanism will incline to one side of the landing electrodes on both sides, thereby adjusting the inclination angle of the micro-mirror surface. Wherein, the MEMS micro-mirror array device can adopt a deformable mirror array as required.

In a preferred embodiment, the imaging target 3 can be a transparent or opaque biological tissue set near the front focal plane of the microscope objective 4 .

In a preferred embodiment, the laser light source 1 can be a planar laser light source.

The fast wide-field-of-view volume holographic fluorescence microscopic imaging system of the present invention can perform single-spectrum imaging or fast multi-spectral imaging according to the characteristics of the imaging target and the requirements of the imaging spectrum. The above two situations will be described in detail through specific examples below:

Embodiment 1: The fast wide-field volume holographic fluorescence microscopic imaging system of the present invention is used to complete single-spectrum imaging, that is, to obtain single-spectrum image information of an imaging target.

When it is necessary to obtain the single-spectrum image information of the imaging target, the deflection angle of each row of micromirrors of the MEMS micromirror array device 51 used for encoding is set according to the matching conditions of the volume holographic grating device 52 and the imaging spectrum wavelength, so that after encoding The imaging light wave can be incident on the volume holographic grating device 52 at a Bragg matching angle, and the light diffracted by the volume holographic grating device 52 is incident on the MEMS micro-mirror decoding array device 53 for decoding, and the decoded single-spectrum light passes through the imaging lens 6 Imaging onto the image sensor array 7 .

As shown in Figure 1(a), the laser light source 1 emits illumination light waves with uniform intensity through the dichroic beamsplitter 2 and the microscope objective lens 4 to the imaging target 3 in turn, and the dichroic beamsplitter 2 filters out the illumination in the fluorescence signal The light, the fluorescence emitted by the imaging target 3 is projected to the MEMS micro-mirror array device 51 through the microscope objective lens 4, and the MEMS micro-mirror array device 51 designs the spatial encoding angle according to the center wavelength of the imaging band and the parameters of the volume holographic grating device 52, Control the deflection direction of the imaging beam. For light of the same wavelength, the inclination angles of the micromirror units in different coding regions on the MEMS micromirror array 51 used for coding are different, and the deflected imaging beam is incident on the transmissive volume holographic grating device 52 at a matching angle. The final light is diffracted by the volume holographic grating device 52 and then enters the MEMS micro-mirror decoding array device 53 to perform spatial angle decoding that is symmetrical to the encoding method, and the decoded monochromatic light is imaged to the image detector array 7 by the imaging lens 6 Obtain wide-field single-spectral images.

The above-mentioned single-spectrum spatial angle encoding principle is as follows: as shown in Figure 5, the light wave emitted by the imaging target 3 passes through the microscopic objective lens 4 and then enters the volume holographic spatial angle encoding and decoding module 5, and the inclined micro-mirror shown in Figure 5 represents the corresponding A column of micromirrors, according to the degeneracy characteristics of the volume holographic grating device 52 and the required spectral width to calculate the spatial angle code and the column number n of micromirrors with the same spatial angle code, n can be calculated by the following formula

In the formula, l is the coded width on the MEMS micro-mirror calculated according to the monochromatic spectral width, and d is the diameter of a single micro-mirror.

The object light is reflected to the corresponding area of the volume holographic grating device 52 through the spatial encoder, and the wavelength of the incident light is the same. For the light waves at different positions in the negative x direction, different spatial angle encoding methods are used, so that the light waves incident on the volume holographic grating device 52 are parallel Evenly arranged image stripes, the stripe width can be calculated by the following formula.

l _i ＝n _i d/cos2θ _i ≈n _i d/2θ _i

In the formula, l _i is the width of the i-th image stripe, d is the diameter of a single micromirror, _θi is the spatial encoding angle, and n _i is the number of columns of micromirrors with the same deflection angle on the micromirror array. By changing the number of coded micromirror columns with the same deflection angle, the spectral width of the imaging single-spectrum light can be adjusted. The monochromatic light diffracted by the volume holographic grating device 52 passes through the MEMS micro-mirror decoding array device 53 for spatial angle decoding, and recovers the spatial angle information before incident on the MEMS micro-mirror array device 51 for spatial encoding.

Embodiment 2: Using the fast wide-field volume holographic fluorescence microscopy imaging system of the present invention to complete fast multi-spectral imaging, that is, to continuously acquire multiple different monochromatic spectral images in which the imaging target changes continuously in a short period of time.

When it is necessary to continuously acquire multiple monochromatic spectral images of the imaging target in a short period of time, according to the matching conditions of the volume holographic grating device 52 and the wavelength parameters of the selected imaging spectrum, the MEMS micromirror array device for encoding is designed 51 The deflection angle and angle switching speed and range of each column of micromirrors are adjusted by adjusting the encoding parameters of the MEMS micromirror array device 51 and the corresponding decoding parameters of the MEMS micromirror decoding array device 53 to realize fast imaging spectrum switching.

As shown in Figure 1(b), the laser light source 1 emits illumination light waves with uniform intensity to the imaging target 3 through the dichroic beam splitter 2 and the microscope objective lens 4 in turn, and the dichroic beam splitter 2 filters out the illumination in the fluorescent signal Light, the fluorescence emitted by the imaging target 3 is projected onto the MEMS micro-mirror array device 51 through the microscope objective lens 4, and the MEMS micro-mirror array device 51 encodes the image space angle, according to different imaging spectrum wavelength parameters and the volume holographic grating device 52 The parameter design space encodes the angle, and controls the inclination angle and angle switching speed of the micromirror in the corresponding column on the MEMS micromirror array device 51. The deflected imaging beam is incident on the reflective volume holographic grating device 52 at a matching angle, and the incident light Diffraction by the reflective volume holographic grating device 52, the MEMS micro-mirror decoding array device 53 spatially decodes the diffracted light, and finally the imaging lens 6 images the decoded diffracted light to the image detector array 7 to sequentially obtain the corresponding wide field of view. Single-spectrum images of spectral bands.

As shown in FIG. 5 , the above-mentioned multi-spectral spatial angle encoding principle is as follows: the light wave emitted by the imaging target 3 enters the MEMS micro-mirror array device 51 through the micro-objective lens 4 . The tilted micromirrors shown in FIG. 6 represent micromirrors corresponding to the same column. The object light wave is projected to the corresponding area of the volume holographic grating device 52 through the MEMS micro-mirror array device 51 . Taking the switching between two different imaging wavelengths as an example, according to the change of the wavelength of the incident light, angle encodings with different inclination angles are used for light waves of different wavelengths, θ ₁ and θ ₂ are MEMS microreflection corresponding to wavelength 1 and wavelength 2 Different spatial encoding angles on the mirror array device 51 . For light of the same wavelength, the inclination angles of the micromirror units in different coded regions on the MEMS micromirror array device 51 are different. According to different positions, as shown in Figure 6, the incident position of light of different wavelengths on the volume holographic grating 52 also changes with the change of the deflection angle of the micro-mirror array, and the light incident on the MEMS micro-mirror array in parallel contains 2 For light of two wavelengths, the exit angle is different before and after the spatial angle encoding switch, and the spatial angle of the Bragg condition incident on the volume holographic grating device will be quickly switched from the matching state of wavelength 1 to the matching state of wavelength 2, and then incident on the volume holographic grating after encoding The stripe width on the device 52 surface can be calculated by the following formula:

l _i ＝n _i d/cos2θ _i ≈n _i d/2θ _i

where l _i is the corresponding image fringe width, d is the diameter of a single micromirror, _θi is the spatial encoding angle, and n _i is the column number of micromirrors with the same deflection angle on the micromirror array.

The above-mentioned embodiments are only used to illustrate the present invention, wherein the structure of each component, the setting position, the connection mode, the structural parameters of the device, the encoding mode and the data processing method all can be changed to some extent. The equivalent transformations and improvements made above shall not be excluded from the protection scope of the present invention.

Claims (3)

1. a kind of quick wide visual field volume holographic fluorescence microimaging systems, it is characterised in that：It include a laser light source, one or two to Color spectroscope, a microcobjective, a MEMS micromirror array devices, a volume holographic grating device, an imaging len and a figure As detector array, wherein the MEMS micromirror array devices are located at the image plane of the microcobjective and are arranged in institute State the incidence end of volume holographic grating device；

The laser light source is used to provide the illumination condition of even intensity for an imageable target, and the laser light source emits illumination light Wave is to the dichroic beamsplitter；The dichroic beamsplitter transfers lighting light wave perpendicular to its incident direction and to pass through institute It states microcobjective and irradiates the imageable target, the fluorescence that the imageable target is sent out returns to the dichroic through the microcobjective Spectroscope, the dichroic beamsplitter filter out the imageable target and send out the lighting light wave contained in fluorescence, the imageable target The fluorescence sent out projects the MEMS micromirror array devices through the microcobjective；The MEMS micro reflector arrays device Part is according to the centre wavelength of imaging spectral and Prague characterisitic parameter of the volume holographic grating device to being located at different location Light wave carry out angular coding and then control the deflection direction of imaging beam, imaging beam after deflection is to match angle incidence To the volume holographic grating device；The volume holographic grating device carries out diffraction to the incident light after space angle encodes, Diffraction light is imaged onto described image detector array through the imaging len；

The volume holographic fluorescence microimaging systems further include one for carrying out the decoded MEMS micro-reflectors decoding of space angle Array device, the MEMS micro-reflectors decoding arrays device are located at the focal plane of the object space of the imaging len and setting In the diffraction exit end of the volume holographic grating device；The volume holographic grating device is to the incidence after space angle encodes Light carries out diffraction and diffraction light is emitted to the MEMS micro-reflectors decoding arrays device, and the MEMS micro-reflectors decode battle array Row device is according to the deflection angle for being used for each row micro-reflector in the MEMS micromirror array devices of space angle coding It controls the driving force for the decoded each row micro-reflector in space, full symmetric angular deflection is realized, to spreading out after coding It penetrates light to be decoded, decoded diffraction light is imaged onto by the MEMS micro-reflectors decoding arrays device through the imaging len Described image detector array.

2. a kind of quick wide visual field volume holographic fluorescence microimaging systems as described in claim 1, it is characterised in that：The body Holographic grating device is multiple using the reflective volume holographic grating device of single channel, single channel transmission-type volume holographic grating device, multichannel wavelength With reflective volume holographic grating device or multichannel wavelength multiplexing transmission-type volume holographic grating device.

3. a kind of quick wide visual field volume holographic fluorescence microimaging systems as claimed in claim 1 or 2, it is characterised in that：Institute State the space angle code encoding/decoding mode of MEMS micromirror array devices and the MEMS micro-reflectors decoding arrays device according to Imageable target feature and imaging spectral demand, specifically include following two forms：

1. when needing to obtain the single-spectral images information of the imageable target, according to the matching item of the volume holographic grating device Part and imaging wavelength control the deflection angle of each row micro-reflector of the MEMS micromirror array devices, make imaging wave energy It is enough that the volume holographic grating device is incident on the matched angle in Prague, by controlling the MEMS micro-reflectors decoding arrays Device obtains the single-spectral images in the wide visual field for the simple spectrum photodissociation code after selection diffraction directly on described image detector；

2. when needing to continuously acquire multiple and different wavelength images of consecutive variations in a short time of the imageable target, according to The wavelength parameter of the matching condition of the volume holographic grating device and the different imaging spectrals of selection controls the micro- reflections of the MEMS The deflection angle of each row micro-reflector of array device, by the deflection angle to the MEMS micromirror array devices into Row coding realizes being switched fast for imaging spectral, and is spread out for selection by controlling the MEMS micro-reflectors decoding arrays device Simple spectrum photodissociation code after penetrating directly sequentially obtains the monochromatic light spectrogram of corresponding wide visual field different spectral coverage on described image detector Picture.