CN105309308A - Orchid culture medium and preparation method thereof - Google Patents
Orchid culture medium and preparation method thereof Download PDFInfo
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- CN105309308A CN105309308A CN201510663390.5A CN201510663390A CN105309308A CN 105309308 A CN105309308 A CN 105309308A CN 201510663390 A CN201510663390 A CN 201510663390A CN 105309308 A CN105309308 A CN 105309308A
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- orchid
- tea
- waste residue
- perlite
- plant growth
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- 241000233855 Orchidaceae Species 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims description 12
- 239000001963 growth medium Substances 0.000 title abstract description 7
- 244000269722 Thea sinensis Species 0.000 claims abstract description 28
- 239000002699 waste material Substances 0.000 claims abstract description 25
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000002351 wastewater Substances 0.000 claims abstract description 22
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 20
- 229930006000 Sucrose Natural products 0.000 claims abstract description 20
- 239000005648 plant growth regulator Substances 0.000 claims abstract description 20
- 229920001817 Agar Polymers 0.000 claims abstract description 19
- 239000008272 agar Substances 0.000 claims abstract description 19
- 235000019362 perlite Nutrition 0.000 claims abstract description 19
- 239000010451 perlite Substances 0.000 claims abstract description 19
- 239000000843 powder Substances 0.000 claims abstract description 19
- 235000015193 tomato juice Nutrition 0.000 claims abstract description 19
- 239000000203 mixture Substances 0.000 claims description 23
- 235000013616 tea Nutrition 0.000 claims description 23
- 239000002609 medium Substances 0.000 claims description 21
- 229910052799 carbon Inorganic materials 0.000 claims description 20
- 239000005720 sucrose Substances 0.000 claims description 19
- 239000002245 particle Substances 0.000 claims description 18
- 239000003245 coal Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 7
- -1 mixes Substances 0.000 claims description 7
- 239000012452 mother liquor Substances 0.000 claims description 7
- 235000006468 Thea sinensis Nutrition 0.000 claims description 4
- 235000020279 black tea Nutrition 0.000 claims description 2
- 235000009569 green tea Nutrition 0.000 claims description 2
- 239000006870 ms-medium Substances 0.000 claims description 2
- 235000020333 oolong tea Nutrition 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- 235000020334 white tea Nutrition 0.000 claims description 2
- 235000020338 yellow tea Nutrition 0.000 claims description 2
- 230000004083 survival effect Effects 0.000 abstract description 3
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 2
- 239000003899 bactericide agent Substances 0.000 abstract description 2
- 230000006378 damage Effects 0.000 abstract description 2
- 235000013681 dietary sucrose Nutrition 0.000 abstract 1
- 229960004793 sucrose Drugs 0.000 abstract 1
- 241000732800 Cymbidium Species 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 244000127818 Phalaenopsis amabilis Species 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 230000035784 germination Effects 0.000 description 2
- 239000004021 humic acid Substances 0.000 description 2
- 239000003864 humus Substances 0.000 description 2
- 239000002932 luster Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 1
- 241001292339 Adenandra uniflora Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000035126 Facies Diseases 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 235000020197 coconut milk Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 125000001477 organic nitrogen group Chemical group 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
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- Cultivation Of Plants (AREA)
Abstract
The invention discloses an orchid culture medium. An MS culture medium is used as a basic culture medium. The orchid culture medium per liter comprises 15-20 g of saccharose, 5-8 g of agar powder, 3-5 g of plant growth regulators, 50-80 ml of filature wastewater, 2-5 g of activated carbon, 20-30 ml of tomato juice, 5-10 g of tea waste residues and 5-10 g of perlite. The culture medium can promote sprouting and rapid propagation of orchid, effectively improve the survival rate of the orchid, reduce the damage of a bactericide on explants, promote axillary bud sprouting and shorten sprouting time.
Description
Technical field
The present invention relates to field of plant tissue culture technique, particularly relate to a kind of orchid medium and preparation method thereof.
Background technology
Orchid is Chinese tradition famous flower, owing to having unique leaf, flower, perfume (or spice), thus has high ornamental value.In recent years, the cymbidium variety such as Moth orchid, hybrid cymbidium also became high-grade indoor pot flowers popular on China flowers market.Moth orchid (PhalaenopsisB1.Spp.) is spent greatly because of it, and the florescence is long, and pattern is gorgeous, and color and luster enriches, and flower shape is beautiful unique, as butterfly dances lightly, thus gains the name, has the laudatory title of " orchid queen " in hot bandwidth, is one of most popular in recent years orchid.Hybrid cymbidium (Cymbidium) be to be grown nonparasitically upon another plant kind by the great Hua in Cymbidium, little Hua hangs down non-hibernating eggs and some Terrestrial orchid through many kinds be bred as artificial hybridization of more than 100 years, the tall and big grandness of its plant type, spends large and many, bright in luster.
Because Moth orchid is single stem aerial orchid, plant seldom grows side shoot, is difficult to adopt conventional plant division mode to carry out vegetative propagation, and its seed is not also containing endosperm or its hetero-organization, and germination rate is extremely low under field conditions (factors), and seeding method therefore cannot be utilized to carry out sexual propagation.And hybrid cymbidium is owing to mostly being crossbreed, seminal propagation cannot keep its quality characteristic, and ripening rate is quite low, plant division ability is weak, causes its reproduction speed slow equally.Therefore, people's majority adopts the method for tissue cultures to carry out Fast-propagation to orchid.But the defects such as existing medium often exists, and planting percent is low, seedling strain growing way is inconsistent, transplanting seedling time is long, and usually need in medium to add expensive coconut milk as nutrients, cost is higher.
Summary of the invention
The object of the present invention is to provide a kind of orchid medium, this medium has that planting percent is high, transplanting seedling time is short, and use silk reeling wastewater and tea waste residue etc. not only reduce production cost and recycle waste material, the medium environmental protection and energy saving again of environmentally friendly property.The technical scheme used for realizing the object of the invention is:
A kind of moth orchid culture medium, medium based on MS medium, also comprises following raw material: sucrose 15-20g/L, agar powder 5-8g/L, plant growth regulator 3-5g/L, silk reeling wastewater 50-80ml/L, active carbon 2-5g/L, Tomato juice 20-30ml/L, tea waste residue 5-10g/L, perlite 5-10g/L.
Be rich in a large amount of ammonia nitrogens, organic nitrogen and pupa albumen in silk reeling wastewater, directly discharge in silk processing factory, river course eutrophication not only can be caused to produce and pollute, and be the significant wastage to resource.
Preferably, described medium also comprises weathered coal 3-8g/L.Containing a large amount of humic acids in weathered coal, humus is the important organic moiety in most of soils, the element composition of humus and coal facies are seemingly, namely primarily of the element such as carbon, hydrogen, oxygen, nitrogen, sulphur composition, be conducive to water in soil, gas, hot situation adjustment and enter the activity of edaphon and enzyme.
Described sucrose 18-20g/L, agar powder 5-7g/L, plant growth regulator 4-5g/L, silk reeling wastewater 55-75ml/L, active carbon 4-5g/L, Tomato juice 25-30ml/L, tea waste residue 5-8g/L, perlite 5-8g/L.
Described sucrose 15-17g/L, agar powder 6-8g/L, plant growth regulator 3-4g/L, silk reeling wastewater 60-70ml/L, active carbon 3-5g/L, Tomato juice 20-25ml/L, tea waste residue 6-9g/L, perlite 6-9g/L.
Described tea waste residue be black tea, green tea, white tea, yellow tea or Oolong tea brewed after, residual tealeaves.
Described tea waste residue is pulverized in advance, is ground into the particle that particle diameter is 1-2mm.
A preparation method for orchid medium, concrete preparation method is:
(1) take agar powder 5-8g to add in the pot filling 1 liter of pure water and boil, add 15-20g sucrose and make it fully dissolve, then get 0.1 liter of MS mother liquor and add in pot;
(2) active carbon, tea waste residue and perlite are pulverized respectively, being ground into particle diameter is 600-800 object particle, mixes, mixture;
(3) mixture in step (2) is placed in the pot of step (1), after cooling, adds plant growth regulator, silk reeling wastewater and Tomato juice, mix, be i.e. finished product.
Useful object of the present invention for: make full use of the nitrogen, P elements and the albumen that are rich in silk reeling wastewater, use the character of weathered coal humic acid, improve soil structure, not only reduce production cost.Adopt sucrose as carbon source in medium, under the prerequisite that orchid survival rate and germination rate are not affected, can effectively reduce costs.In medium of the present invention, plant growth regulator can promote the rudiment of orchid, makes its Fast-propagation, effectively can improve the survival rate of orchid, reduces bactericide to the injury of explant, promotes axillary bud sprouting, shorten sprout time.
Embodiment
Embodiment 1
Sucrose 20g/L, agar powder 8g/L, plant growth regulator 3g/L, silk reeling wastewater 50ml/L, active carbon 5g/L, Tomato juice 30ml/L, tea waste residue 5g/L, perlite 5g/L, concrete preparation method is:
(1) take 8g agar powder to add in the pot filling 1 liter of pure water and boil, add 20g sucrose and make it fully dissolve, then get 0.1 liter of MS mother liquor and add in pot;
(2) 5g active carbon, 5g tea waste residue and 5g perlite are pulverized respectively, being ground into particle diameter is 600 object particles, mixes, mixture;
(3) mixture in step (2) is placed in the pot of step (1), after cooling, adds 3g plant growth regulator, 50ml silk reeling wastewater and 30ml Tomato juice, mix, i.e. finished product.
Embodiment 2
Sucrose 15g/L, agar powder 5g/L, plant growth regulator 5g/L, silk reeling wastewater 80ml/L, active carbon 2g/L, Tomato juice 20ml/L, tea waste residue 10g/L, perlite 10g/L, concrete preparation method is:
(1) take agar powder 5g to add in the pot filling 1 liter of pure water and boil, add 15g sucrose and make it fully dissolve, then get 0.1 liter of MS mother liquor and add in pot;
(2) 2g active carbon, 10g tea waste residue and 10g perlite are pulverized respectively, being ground into particle diameter is 600-800 object particle, mixes, mixture;
(3) mixture in step (2) is placed in the pot of step (1), after cooling, adds 5g plant growth regulator, 80ml silk reeling wastewater and 20ml Tomato juice, mix, i.e. finished product.
Embodiment 3
Sucrose 18g/L, agar powder 7g/L, plant growth regulator 4g/L, silk reeling wastewater 60ml/L, active carbon 4g/L, Tomato juice 25ml/L, tea waste residue 8g/L, perlite 7g/L, concrete preparation method is:
(1) take agar powder 7g to add in the pot filling 1 liter of pure water and boil, add 18g sucrose and make it fully dissolve, then get 0.1 liter of MS mother liquor and add in pot;
(2) 4g active carbon, 8g tea waste residue and 7g perlite are pulverized respectively, being ground into particle diameter is 600-800 object particle, mixes, mixture;
(3) mixture in step (2) is placed in the pot of step (1), after cooling, adds 4g plant growth regulator, 60ml silk reeling wastewater and 25ml Tomato juice, mix, i.e. finished product.
Embodiment 4
Sucrose 17g/L, agar powder 6g/L, plant growth regulator 3g/L, silk reeling wastewater 55ml/L, active carbon 4g/L, Tomato juice 28ml/L, tea waste residue 9g/L, perlite 8g/L, weathered coal 3g/L, concrete preparation method is:
(1) take agar powder 6g to add in the pot filling 1 liter of pure water and boil, add 17g sucrose and make it fully dissolve, then get 0.1 liter of MS mother liquor and add in pot;
(2) 4g active carbon, 3g weathered coal, 9g tea waste residue and 8g/ perlite are pulverized respectively, being ground into particle diameter is 600-800 object particle, mixes, mixture;
(3) mixture in step (2) is placed in the pot of step (1), after cooling, adds 3g plant growth regulator, 55ml silk reeling wastewater and 28ml Tomato juice, mix, i.e. finished product.
Embodiment 5
Sucrose 19g/L, agar powder 6g/L, plant growth regulator 4g/L, silk reeling wastewater 75ml/L, active carbon 3g/L, Tomato juice 27ml/L, tea waste residue 9g/L, perlite 6g/L, weathered coal 8g/L, concrete preparation method is:
(1) take agar powder 6g to add in the pot filling 1 liter of pure water and boil, add 19g sucrose and make it fully dissolve, then get 0.1 liter of MS mother liquor and add in pot;
(2) 3g active carbon, 8g weathered coal, 9g tea waste residue and 6g perlite are pulverized respectively, being ground into particle diameter is 600-800 object particle, mixes, mixture;
(3) mixture in step (2) is placed in the pot of step (1), after cooling, adds 4g plant growth regulator, 75ml silk reeling wastewater and 27ml Tomato juice, mix, i.e. finished product.
Claims (7)
1. an orchid medium, medium based on MS medium, it is characterized in that, also comprise following raw material: sucrose 15-20g/L, agar powder 5-8g/L, plant growth regulator 3-5g/L, silk reeling wastewater 50-80ml/L, active carbon 2-5g/L, Tomato juice 20-30ml/L, tea waste residue 5-10g/L, perlite 5-10g/L.
2. orchid medium according to claim 1, is characterized in that, described medium also comprises weathered coal 3-8g/L.
3. orchid medium according to claim 1, it is characterized in that, described sucrose 18-20g/L, agar powder 5-7g/L, plant growth regulator 4-5g/L, silk reeling wastewater 55-75ml/L, active carbon 4-5g/L, Tomato juice 25-30ml/L, tea waste residue 5-8g/L, perlite 5-8g/L.
4. orchid medium according to claim 1, it is characterized in that, described sucrose 15-17g/L, agar powder 6-8g/L, plant growth regulator 3-4g/L, silk reeling wastewater 60-70ml/L, active carbon 3-5g/L, Tomato juice 20-25ml/L, tea waste residue 6-9g/L, perlite 6-9g/L.
5. orchid medium according to claim 1, is characterized in that, described tea waste residue be black tea, green tea, white tea, yellow tea or Oolong tea brewed after, residual tealeaves.
6. orchid medium according to claim 1, is characterized in that, described tea waste residue is pulverized in advance, and being ground into particle diameter is 600-800 object particle.
7. the preparation method of orchid medium according to claim 1, is characterized in that, concrete preparation method is,
(1) take agar powder 5-8g to add in the pot filling 1 liter of pure water and boil, add 15-20g sucrose and make it fully dissolve, then get 0.1 liter of MS mother liquor and add in pot;
(2) active carbon, tea waste residue and perlite are pulverized respectively, being ground into particle diameter is 600-800 object particle, mixes, mixture;
(3) mixture in step (2) is placed in the pot of step (1), after cooling, adds plant growth regulator, silk reeling wastewater and Tomato juice, mix, be i.e. finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510663390.5A CN105309308A (en) | 2015-10-15 | 2015-10-15 | Orchid culture medium and preparation method thereof |
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| CN201510663390.5A CN105309308A (en) | 2015-10-15 | 2015-10-15 | Orchid culture medium and preparation method thereof |
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| CN105309308A true CN105309308A (en) | 2016-02-10 |
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Cited By (2)
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|---|---|---|---|---|
| CN106718260A (en) * | 2016-11-24 | 2017-05-31 | 广西炎瑞农业发展有限公司 | A kind of podocarpus culture medium and preparation method thereof |
| CN106718261A (en) * | 2016-11-24 | 2017-05-31 | 广西炎瑞农业发展有限公司 | A kind of Huanghua Pear culture medium and preparation method thereof |
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2015
- 2015-10-15 CN CN201510663390.5A patent/CN105309308A/en active Pending
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Application publication date: 20160210 |