CN103250638A - Chrysanthemum tissue culture method - Google Patents
Chrysanthemum tissue culture method Download PDFInfo
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- CN103250638A CN103250638A CN2013101334433A CN201310133443A CN103250638A CN 103250638 A CN103250638 A CN 103250638A CN 2013101334433 A CN2013101334433 A CN 2013101334433A CN 201310133443 A CN201310133443 A CN 201310133443A CN 103250638 A CN103250638 A CN 103250638A
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- chrysanthemum
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- tissue culture
- seedling
- raw material
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 241000723353 Chrysanthemum Species 0.000 title claims abstract description 34
- 235000007516 Chrysanthemum Nutrition 0.000 title claims abstract description 33
- 238000012136 culture method Methods 0.000 title abstract 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000003337 fertilizer Substances 0.000 claims abstract description 12
- 239000002994 raw material Substances 0.000 claims description 12
- 239000002689 soil Substances 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 9
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 238000012364 cultivation method Methods 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 235000015097 nutrients Nutrition 0.000 claims description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 6
- 238000001467 acupuncture Methods 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
- 241000609240 Ambelania acida Species 0.000 claims description 3
- 241000255789 Bombyx mori Species 0.000 claims description 3
- 240000002791 Brassica napus Species 0.000 claims description 3
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims description 3
- 241000233866 Fungi Species 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 3
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 229940037003 alum Drugs 0.000 claims description 3
- 239000010905 bagasse Substances 0.000 claims description 3
- 239000004202 carbamide Substances 0.000 claims description 3
- KLOIYEQEVSIOOO-UHFFFAOYSA-N carbocromen Chemical compound CC1=C(CCN(CC)CC)C(=O)OC2=CC(OCC(=O)OCC)=CC=C21 KLOIYEQEVSIOOO-UHFFFAOYSA-N 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 239000003245 coal Substances 0.000 claims description 3
- 238000009264 composting Methods 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 210000003608 fece Anatomy 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 claims description 3
- 230000004151 fermentation Effects 0.000 claims description 3
- 238000000227 grinding Methods 0.000 claims description 3
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000010871 livestock manure Substances 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- 229960002523 mercuric chloride Drugs 0.000 claims description 3
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 claims description 3
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000002417 nutraceutical Substances 0.000 claims description 3
- 235000021436 nutraceutical agent Nutrition 0.000 claims description 3
- 239000011368 organic material Substances 0.000 claims description 3
- 239000010451 perlite Substances 0.000 claims description 3
- 235000019362 perlite Nutrition 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 239000002893 slag Substances 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- 239000010455 vermiculite Substances 0.000 claims description 3
- 235000019354 vermiculite Nutrition 0.000 claims description 3
- 229910052902 vermiculite Inorganic materials 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 239000000811 xylitol Substances 0.000 claims description 3
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims description 3
- 229960002675 xylitol Drugs 0.000 claims description 3
- 235000010447 xylitol Nutrition 0.000 claims description 3
- 244000269722 Thea sinensis Species 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract description 3
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 241000700605 Viruses Species 0.000 abstract 1
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 230000004720 fertilization Effects 0.000 abstract 1
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 239000005974 Chlormequat Substances 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- JUZXDNPBRPUIOR-UHFFFAOYSA-N chlormequat Chemical compound C[N+](C)(C)CCCl JUZXDNPBRPUIOR-UHFFFAOYSA-N 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000007407 health benefit Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
Landscapes
- Fertilizers (AREA)
Abstract
The invention discloses a chrysanthemum tissue culture method, which comprises the following steps of: tissue culture and water control: during the excessive growth period of chrysanthemum, carrying out water control, so that the chrysanthemum is devitalized; needle piercing: piercing the stem of the lower end of a leaf at a growing point by using a sterilized needle; and fertilization. According to the chrysanthemum tissue culture method disclosed by the invention, the inherent properties and features of original varieties are kept, the reproduction speed is high, the land resource is saved, and an effect of no virus and no pollution can be achieved; through the methods such as needle piercing, dwarfing chrysanthemum can be obtained, thereby enhancing the ornamental value; and through the addition of various fertilizers, the self nutritional value of chrysanthemum is enhanced, and the chrysanthemum is beneficial to the physical health in case of being used for making tea.
Description
Technical field
The present invention relates generally to a kind of chrysanthemum tissue and cultivates cultivation method, belongs to field of planting.
Background technology
Cultivating chrysanthemum, often to control its height, the strain shape attractive in appearance to obtain compact, have only short just can the making of strain to spend greatly, in great numbers, suitable indoor ornaments, the method that traditional chrysanthemum is downgraded is to postpone cutting time mostly, pinching, regularly spray chlormequat etc., complicated operation, the family planting chrysanthemum is also more and more general now, and the chrysanthemum of kind not only can be used for viewing and admiring, can also be used to making tea etc., therefore the nutritive value that strengthens chrysanthemum also is necessary, cultivates by tissue and obtains the chrysanthemum seedling, has not only kept intrinsic properties and the characteristic of original kind, can also economize the land resource, and virus-free.
Summary of the invention
The object of the invention is exactly in order to provide a kind of chrysanthemum to downgrade processing method.
The present invention is achieved by the following technical solutions:
A kind of chrysanthemum tissue is cultivated cultivation method, and it may further comprise the steps:
(1) tissue is cultivated: get the chrysanthemum spray as explant, use aseptic water washing 4-5 time earlier, with the mercuric chloride solution sterilization of 0.1-0.2%, being inoculated into afterwards in the medium then, is to carry out aseptic culture under 20-22 ℃ in temperature, to taking root, seedling, seedling is taken out the back clean root with clear water, be transplanted in the matrix that sterilization vermiculite and perlite by 1:2-3 mix and cultivate, general incubation time is 5-15 days, can be transplanted in the soil after waiting seedling to become strong;
The weight portion (g) of contained each raw material is in every liter of described medium:
Agar 10-12, dusty yeast 4-5, sucrose 20-30, diammonium hydrogen citrate 1-3, potassium dihydrogen phosphate 2-3, ferrous sulfate heptahydrate 0.2-0.4, remaining be distilled water;
(2) transplant: in the time of transplanting, nutrients is admixed in the basin soil uniformly, nutraceutical consumption is the 2-3% of basin soil, and described nutrients is made up of the raw material mixing grinding of following weight parts:
Silkworm excrement 8-10, brewer's grains 6-8, xylitol slag 2-4, weathered coal 1-2;
(3) control water: chrysanthemum excessive growth period control water, here make it to beat;
(4) acupuncture: in the blade lower end of growing point, use the needle-penetration stem through sterilization;
(5) fertilising: every basin application of organic fertilizers 0.4-0.6kg, bloomed previous month, every 3-5 days, urea, the potassium dihydrogen phosphate of 0.04-0.06%, the mixed liquor that can impose 0.1-0.2% were made liquid manure;
Described fertilizer is made up of the raw material of following weight parts:
Edible fungi residues 30-40, poultry-dung 30-40, distiller's dried grain 8-10, system chaff 8-10, bagasse 4-6, alum powder 2-4, rapeseed dregs 4-5;
Above-mentioned raw materials is mixed the organic material composting agent that grinds back adding 1-3%, add water-water reactor and put fermentation, namely get described fertilizer.
Advantage of the present invention is:
Chrysanthemum tissue of the present invention is cultivated cultivation method, not only keeps intrinsic properties and the characteristic of original kind, and reproduction speed is fast, economize the land resource, can be virus-free, pollution-free, by methods such as acupunctures, the chrysanthemum that can obtain to downgrade, strengthen sight, by the various fertilizer that add, strengthened the nutritive value of chrysanthemum self again, be used for making tea body health benefits.
Embodiment
Embodiment 1
A kind of chrysanthemum tissue is cultivated cultivation method, and it may further comprise the steps:
(1) tissue is cultivated: get the chrysanthemum spray as explant, use aseptic water washing 5 times earlier, with the mercuric chloride solution sterilization of 0.1-0.2%, being inoculated into afterwards in the medium then, is to carry out aseptic culture under 22 ℃ in temperature, to taking root, seedling, seedling is taken out the back clean root with clear water, be transplanted in the matrix that sterilization vermiculite and perlite by 1:3 mix and cultivate, general incubation time is 15 days, can be transplanted in the soil after waiting seedling to become strong;
The weight portion (g) of contained each raw material is in every liter of described medium:
Agar 12, dusty yeast 5, sucrose 30, diammonium hydrogen citrate 3, potassium dihydrogen phosphate 3, ferrous sulfate heptahydrate 0.4, remaining be distilled water;
(2) transplant: in the time of transplanting, nutrients is admixed in the basin soil uniformly, nutraceutical consumption is 3% of basin soil, and described nutrients is made up of the raw material mixing grinding of following weight parts:
Silkworm excrement 10, brewer's grains 8, xylitol slag 4, weathered coal 2;
(3) control water: chrysanthemum excessive growth period control water, here make it to beat;
(4) acupuncture: in the blade lower end of growing point, use the needle-penetration stem through sterilization;
(5) fertilising: every basin application of organic fertilizers 0.6kg, it is previous month to bloom, and every 5 days, can impose 0.2% urea, 0.06% potassium dihydrogen phosphate, mixed liquor made liquid manure;
Described fertilizer is made up of the raw material of following weight parts:
Edible fungi residues 40, poultry-dung 40, distiller's dried grain 10, system chaff 10, bagasse 6, alum powder 4, rapeseed dregs 5;
Above-mentioned raw materials is mixed the organic material composting agent that grinds back adding 1-3%, add water-water reactor and put fermentation, namely get described fertilizer.
The chrysanthemum plant height that the present invention produces is no more than 35 centimetres.The chrysanthemum quality that the present invention grows is good, it is dense to grow, growing power is strong, reception luminosity height, the diseases prevention performance is good, belongs to 100% natural green chrysanthemum, effectively overcome because the deficiency of various nutrition fertilizer, easily cause chrysanthemum blade face Huang, the rareness of blooming, longer chrysanthemum stature little, premunition is poor, easily suffers from the defective of various damage by disease and insect.
Claims (1)
1. a chrysanthemum tissue is cultivated cultivation method, it is characterized in that it may further comprise the steps:
(1) tissue is cultivated: get the chrysanthemum spray as explant, use aseptic water washing 4-5 time earlier, with the mercuric chloride solution sterilization of 0.1-0.2%, being inoculated into afterwards in the medium then, is to carry out aseptic culture under 20-22 ℃ in temperature, to taking root, seedling, seedling is taken out the back clean root with clear water, be transplanted in the matrix that sterilization vermiculite and perlite by 1:2-3 mix and cultivate, general incubation time is 5-15 days, can be transplanted in the soil after waiting seedling to become strong;
The weight portion (g) of contained each raw material is in every liter of described medium:
Agar 10-12, dusty yeast 4-5, sucrose 20-30, diammonium hydrogen citrate 1-3, potassium dihydrogen phosphate 2-3, ferrous sulfate heptahydrate 0.2-0.4, remaining be distilled water;
(2) transplant: in the time of transplanting, nutrients is admixed in the basin soil uniformly, nutraceutical consumption is the 2-3% of basin soil, and described nutrients is made up of the raw material mixing grinding of following weight parts:
Silkworm excrement 8-10, brewer's grains 6-8, xylitol slag 2-4, weathered coal 1-2;
(3) control water: chrysanthemum excessive growth period control water, here make it to beat;
(4) acupuncture: in the blade lower end of growing point, use the needle-penetration stem through sterilization;
(5) fertilising: every basin application of organic fertilizers 0.4-0.6kg, bloomed previous month, every 3-5 days, urea, the potassium dihydrogen phosphate of 0.04-0.06%, the mixed liquor that can impose 0.1-0.2% were made liquid manure;
Described fertilizer is made up of the raw material of following weight parts:
Edible fungi residues 30-40, poultry-dung 30-40, distiller's dried grain 8-10, system chaff 8-10, bagasse 4-6, alum powder 2-4, rapeseed dregs 4-5;
Above-mentioned raw materials is mixed the organic material composting agent that grinds back adding 1-3%, add water-water reactor and put fermentation, namely get described fertilizer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310133443.3A CN103250638B (en) | 2013-04-17 | 2013-04-17 | A kind of chrysanthemum tissue culture method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310133443.3A CN103250638B (en) | 2013-04-17 | 2013-04-17 | A kind of chrysanthemum tissue culture method |
Publications (2)
| Publication Number | Publication Date |
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| CN103250638A true CN103250638A (en) | 2013-08-21 |
| CN103250638B CN103250638B (en) | 2016-08-31 |
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| CN201310133443.3A Expired - Fee Related CN103250638B (en) | 2013-04-17 | 2013-04-17 | A kind of chrysanthemum tissue culture method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105660401A (en) * | 2016-01-27 | 2016-06-15 | 漳州市萌得尔农业科技有限公司 | Culture medium for tissue-culture anoectochilus roxburghii (Wall.)Lindl as well as preparation method and application of culture medium |
| CN106688884A (en) * | 2016-11-18 | 2017-05-24 | 曹才基 | Chrysanthemum cultivation method |
| CN107295868A (en) * | 2017-05-27 | 2017-10-27 | 句容市农业技术推广中心 | A kind of cultural method of potted dwarf chrysanthemum |
| CN107568048A (en) * | 2017-09-26 | 2018-01-12 | 界首市方辉家庭农场 | A kind of Dwarfing of Chrysanthemum cottage method |
| CN115380817A (en) * | 2022-09-23 | 2022-11-25 | 南宁师范大学 | Pollination robot |
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| JP2003111515A (en) * | 2001-10-03 | 2003-04-15 | Nettai Shigen Shokubutsu Kenkyusho:Kk | Method for cultivating flowering plant into dwarf form |
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2013
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105660401A (en) * | 2016-01-27 | 2016-06-15 | 漳州市萌得尔农业科技有限公司 | Culture medium for tissue-culture anoectochilus roxburghii (Wall.)Lindl as well as preparation method and application of culture medium |
| CN105660401B (en) * | 2016-01-27 | 2018-02-13 | 漳州市萌得尔农业科技有限公司 | A kind of tissue culture roxburgh anoectochilus terminal bud culture medium and its preparation method and application |
| CN106688884A (en) * | 2016-11-18 | 2017-05-24 | 曹才基 | Chrysanthemum cultivation method |
| CN107295868A (en) * | 2017-05-27 | 2017-10-27 | 句容市农业技术推广中心 | A kind of cultural method of potted dwarf chrysanthemum |
| CN107568048A (en) * | 2017-09-26 | 2018-01-12 | 界首市方辉家庭农场 | A kind of Dwarfing of Chrysanthemum cottage method |
| CN115380817A (en) * | 2022-09-23 | 2022-11-25 | 南宁师范大学 | Pollination robot |
| CN115380817B (en) * | 2022-09-23 | 2024-05-14 | 南宁师范大学 | A pollination robot |
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| CN103250638B (en) | 2016-08-31 |
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Effective date of registration: 20190313 Address after: 418000 Bajia Village, Xinjian Town, Zhongfang County, Huaihua City, Hunan Province Patentee after: Dafengyuan Ecological Agriculture Development Co., Ltd. Address before: Room 166, building 4, agricultural products wholesale market, apricot village, Guichi District, Chizhou, Anhui Patentee before: Chizhou Tianjian Ecological Agriculture Development Co., Ltd. |
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Granted publication date: 20160831 Termination date: 20210417 |