CN105203532A - Detection method for urea and total ammonia nitrogen in bean sprouts - Google Patents
Detection method for urea and total ammonia nitrogen in bean sprouts Download PDFInfo
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- CN105203532A CN105203532A CN201510582321.1A CN201510582321A CN105203532A CN 105203532 A CN105203532 A CN 105203532A CN 201510582321 A CN201510582321 A CN 201510582321A CN 105203532 A CN105203532 A CN 105203532A
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- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 title claims abstract description 94
- 239000004202 carbamide Substances 0.000 title claims abstract description 57
- 244000046052 Phaseolus vulgaris Species 0.000 title claims abstract description 49
- 235000010627 Phaseolus vulgaris Nutrition 0.000 title claims abstract description 49
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 title claims abstract description 26
- 238000001514 detection method Methods 0.000 title claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000006228 supernatant Substances 0.000 claims abstract description 11
- 108010046334 Urease Proteins 0.000 claims abstract description 6
- 230000000694 effects Effects 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 24
- 239000007788 liquid Substances 0.000 claims description 19
- 244000068988 Glycine max Species 0.000 claims description 12
- 235000010469 Glycine max Nutrition 0.000 claims description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims description 12
- 238000006555 catalytic reaction Methods 0.000 claims description 5
- 239000000376 reactant Substances 0.000 claims description 4
- 241000532370 Atla Species 0.000 claims description 3
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000010009 beating Methods 0.000 claims description 2
- 239000000284 extract Substances 0.000 claims description 2
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 2
- 230000035945 sensitivity Effects 0.000 abstract description 6
- 239000003795 chemical substances by application Substances 0.000 abstract description 3
- 239000003086 colorant Substances 0.000 abstract description 2
- 238000011161 development Methods 0.000 abstract description 2
- 238000002791 soaking Methods 0.000 abstract description 2
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 235000013877 carbamide Nutrition 0.000 description 37
- ZWLUXSQADUDCSB-UHFFFAOYSA-N phthalaldehyde Chemical compound O=CC1=CC=CC=C1C=O ZWLUXSQADUDCSB-UHFFFAOYSA-N 0.000 description 4
- FZRCKLPSHGTOAU-UHFFFAOYSA-N 6-amino-1,4-dimethylcyclohexa-2,4-diene-1-carbaldehyde Chemical compound CC1=CC(N)C(C)(C=O)C=C1 FZRCKLPSHGTOAU-UHFFFAOYSA-N 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- -1 nitrogenous small-molecule Chemical class 0.000 description 3
- 229940054441 o-phthalaldehyde Drugs 0.000 description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000002696 acid base indicator Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- FSEUPUDHEBLWJY-HWKANZROSA-N diacetylmonoxime Chemical compound CC(=O)C(\C)=N\O FSEUPUDHEBLWJY-HWKANZROSA-N 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- XKLJHFLUAHKGGU-UHFFFAOYSA-N nitrous amide Chemical compound ON=N XKLJHFLUAHKGGU-UHFFFAOYSA-N 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N Aminoantipyrine Natural products CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 1
- 208000037157 Azotemia Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- QSJXEFYPDANLFS-UHFFFAOYSA-N Diacetyl Chemical group CC(=O)C(C)=O QSJXEFYPDANLFS-UHFFFAOYSA-N 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- LSQXNMXDFRRDSJ-UHFFFAOYSA-N Thymol methyl ether Chemical compound COC1=CC(C)=CC=C1C(C)C LSQXNMXDFRRDSJ-UHFFFAOYSA-N 0.000 description 1
- 239000003929 acidic solution Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 150000003868 ammonium compounds Chemical class 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- VEQOALNAAJBPNY-UHFFFAOYSA-N antipyrine Chemical compound CN1C(C)=CC(=O)N1C1=CC=CC=C1 VEQOALNAAJBPNY-UHFFFAOYSA-N 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000008139 complexing agent Substances 0.000 description 1
- LBJNMUFDOHXDFG-UHFFFAOYSA-N copper;hydrate Chemical compound O.[Cu].[Cu] LBJNMUFDOHXDFG-UHFFFAOYSA-N 0.000 description 1
- OBRMNDMBJQTZHV-UHFFFAOYSA-N cresol red Chemical compound C1=C(O)C(C)=CC(C2(C3=CC=CC=C3S(=O)(=O)O2)C=2C=C(C)C(O)=CC=2)=C1 OBRMNDMBJQTZHV-UHFFFAOYSA-N 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- XEYBHCRIKKKOSS-UHFFFAOYSA-N disodium;azanylidyneoxidanium;iron(2+);pentacyanide Chemical compound [Na+].[Na+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].[O+]#N XEYBHCRIKKKOSS-UHFFFAOYSA-N 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- FPVGTPBMTFTMRT-NSKUCRDLSA-L fast yellow Chemical class [Na+].[Na+].C1=C(S([O-])(=O)=O)C(N)=CC=C1\N=N\C1=CC=C(S([O-])(=O)=O)C=C1 FPVGTPBMTFTMRT-NSKUCRDLSA-L 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- XDBSEZHMWGHVIL-UHFFFAOYSA-M hydroxy(dioxo)vanadium Chemical class O[V](=O)=O XDBSEZHMWGHVIL-UHFFFAOYSA-M 0.000 description 1
- RSAZYXZUJROYKR-UHFFFAOYSA-N indophenol Chemical compound C1=CC(O)=CC=C1N=C1C=CC(=O)C=C1 RSAZYXZUJROYKR-UHFFFAOYSA-N 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229960005222 phenazone Drugs 0.000 description 1
- SCWKRWCUMCMVPW-UHFFFAOYSA-N phenyl n-methylcarbamate Chemical group CNC(=O)OC1=CC=CC=C1 SCWKRWCUMCMVPW-UHFFFAOYSA-N 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229940083618 sodium nitroprusside Drugs 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- SEEPANYCNGTZFQ-UHFFFAOYSA-N sulfadiazine Chemical compound C1=CC(N)=CC=C1S(=O)(=O)NC1=NC=CC=N1 SEEPANYCNGTZFQ-UHFFFAOYSA-N 0.000 description 1
- 208000009852 uremia Diseases 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to a detection method for urea and total ammonia nitrogen in bean sprouts. The detection method comprises the steps of chopping the bean sprouts, soaking the chopped bean sprouts with water, adding urease into a supernatant so as to catalyze the supernatant, adding a color developing agent to carry out color development, and comparing colors with a color gradation card, thereby obtaining urea content. The use amount ratio of a sample to the urease to the color developing agent is 0.5mL: (20-200)uL: (20-200)uL, and the enzyme activity of the urease is 30-180U/mL. The detection method provided by the invention is low in cost and high in sensitivity and can be used for rapidly, accurately and conveniently detecting urea content greater than or equal to 3mg/L and ammonia nitrogen content greater than or equal to 1.5mg/L.
Description
Technical field
Chemical field of the present invention, particularly relates to urea and total ammonia nitrogen detection method in a kind of bean sprouts.
Background technology
The normal production cycles of bean sprouts mostly is about 11 ~ 15 days, but some retailers are in order to improve bean sprouts output, shortens the production cycle, sprays the chemical fertilizer such as urea, ammonium nitrate, ammonium sulfate and root-free agent and hormone expedites the emergence of on bean sprouts.Urea is the metabolic product of human normal, it is a kind of nitrogenous small-molecule substance, can urinate along with human body and discharge, a small amount of urea can't work the mischief to human body, when urea is assembled in a large number and can not be discharged, damage can be caused to human body, mainly make the cylinder electrolyte balance of human body destroyed, thus generation disease, i.e. uremia.These ammonium compounds in addition, under the effect of bacterium, can be transformed into nitrosamine, and nitrosamine are a kind of carcinogens.Strengthen the monitoring work to bud dish, seek the market demand that quick screening method is fast and accurately the screening of a large amount of sample.
At present, urea context of detection mainly contains several method:
1) Diacetylmonoxime development process
Diacetyl monoxime method principle is: strong acid heating condition under, urea and diacetyl react generate redness former diazine, shade and urea content proportional.This method reagent used is easy to get, and cost is low, but needs heating when detecting operation, and the range of linearity is low, is not generally suitable for field quick detection.The more important thing is that this kind of method is in bean sprouts urea measures, in bean sprouts, composition interference is comparatively large, easily causes false positive.
2) o-phthalaldehyde method
O-phthalaldehyde method measures urea principle: in acid condition, and the bond of urea and o-phthalaldehyde(OPA), under antipyrine existent condition, generates yellow compound, and the compound of this yellow and metavanadic acid root knot are closed in blue.This method does not need to boil, and simple to operate, disturbing factor is few, but the method reagent is not easily preserved for a long time, is unsuitable for make and examines product soon.
3) paradime thylaminobenzaldehyde method
Paradime thylaminobenzaldehyde method principle is: in an acidic solution, and urea and paradime thylaminobenzaldehyde generate the diaminobenzene formaldehyde urea of lemon yellow.The method stability, linearly good, interference less, easy to operate, but its sensitivity is not high, and the urea being generally used for the aspect such as dairy products, feed detects.
4) nitrite-Griess reagent method
Urea and nitrite react generation nitrogen under strongly acidic conditions, and Griess reagent and nitrite react and generate red compound.Reactant liquor redness containing urea can be partially light, and the color according to solution colour and titer judges whether to there is urea.It is qualitative that the method can be used for urea, and the sensitivity of reaction is 0.2%, does not reach the mensuration requirement of urea in bean sprouts.
Consider the defect of the aspects such as above method reagent stability, material interference, sensitivity, convenience, find a kind of easy and simple to handle, highly sensitive, result is accurate, cost is low, practical bean sprouts carbamide detection method is fast a large amount of sample screening in food security aspect market demand.Urase is a kind of urea decomposition catalyzer efficiently, urea generates NH4+ through urase enzymolysis, and according to ammonium radical ion hydrolysis PH change, the acid base indicator that causes is as variable colors such as cresol red, adjacent phenyl methylcarbamate complexing agent, methyl thymol blues or to survey NH4+ to urea indirect quantification by methods such as salicylic acid-indophenol blue, phenol-indophenol blues be one of study hotspot.
Acid base indicator Indirect Determination and liquid PH to be measured, damping fluid surge capability etc. have very large relation, and its detection is limited to more than 20mg/L urea.Salicylic acid-indophenol blue, phenol-ammonia-nitrogen principle is that sodium nitroprusside catalyzing N H4+ and phenol and hypochlorite react and generate blue compound, according to shade to urea indirect quantification, this kind of method is highly sensitive, but the reaction time is longer, is unsuitable for do and sieves product soon.And Na's reagent and NH4+ are swift in response, get final product colorimetric after shaking up, urase is combined with nessler reagent indirectly to be surveyed urea content and substantially increases detection sensitivity and fast screen capacity.
Summary of the invention
The object of the invention is the defect overcoming the aspects such as above method reagent stability, material interference, sensitivity, convenience, provide a kind of easy and simple to handle, highly sensitive, result is accurate, cost is low, practicality urea and total ammonia nitrogen detection method thereof in bean sprouts fast.
Technical scheme of the present invention is:
A detection method for urea and total ammonia nitrogen in bean sprouts, it, by detecting the ammonia nitrogen produced through urase decomposing urea, carries out sxemiquantitative according to ammonia-nitrogen content to urea, and its method detecting urea and total ammonia nitrogen comprises the following steps:
1) detecting thing is bean sprouts, is shredded bean sprouts, adds water and mixes, and room temperature is placed, and centre is shaken frequently, and obtaining supernatant is liquid to be measured;
2) get two parts respectively and detect liquid, portion adds urase, and another part adds isopyknic water, shakes up, room temperature catalysis;
3) to step 2) described in two parts of reactant liquors in add nessler reagent respectively, shake up;
4) step 3) shake up, observing response liquid color, contrasts with color scale card, to urea content sxemiquantitative.
Urase is beans urase; Described beans urase extracts from soya bean, green soya bean, black soya bean.
The preparation method of described beans urase is: in beans, add water, soaked overnight, and add ethanol water making beating, centrifugal, supernatant is beans urase liquid, and supernatant vacuum freeze drying is obtained beans urase.
Step 1) ratio of bean sprouts and water is 1:1-1:10.
Step 1) room temperature standing time is 2-15min.
Step 2) sample and urase amount ratio be 0.5mL:20-200uL.
Step 2) urease activity is 30 ~ 180UmL
-1.
Step 2) urase catalysis time is 2-10min.
Step 3) consumption of developer is 20-200uL.
Step 4) shake up after, contrasts with color scale card in 0-10min.
Step 4) contrast with color scale card, in liquid to be measured urea content for add urase group and do not add urase group with the difference of colour atla respective value, NH in liquid to be measured
4the content of-N is do not add urase group and color scale card contrasts the numerical value * 0.5 obtained.
Embodiment
Process when the present embodiment is mainly enumerated using soya bean urase as detection reagent.
Bean sprouts urea and total ammonia nitrogen quick detection reagent comprise urase and developer.
Urase is soya bean urase.
The preparation method of urase: soybean soaking is spent the night, adds ethanol water in soya bean, and pulverize, centrifugal, supernatant is soya bean urase liquid, and supernatant freeze drying is obtained soya bean urase.
Developer is made up of following mass percent: NaOH 16%, mercuric iodixde 10%, potassium iodide 7%, and surplus is water.
The method for quick of bean sprouts urea and total ammonia nitrogen, its step is as follows:
1) detecting thing is bean sprouts, is shredded bean sprouts, adds water and mixes, and room temperature places 2-15min, and centre is shaken frequently, and obtaining supernatant is liquid to be measured;
2) get two parts respectively and detect liquid, portion adds urase, and another part adds isopyknic water, shakes up, room temperature catalysis 2-10min;
3) to step 2) add developer in reactant liquor, shake up;
4) step 3) shake up, in 0-10min, observing response liquid color, contrasts with color scale card, to urea content sxemiquantitative.
Step 1) ratio of bean sprouts and water is 1:1-1:10.
Step 2) sample and urase amount ratio be 0.5mL:20-200uL.
Step 2) urease activity is 30 ~ 180UmL-1.
Step 3) consumption of developer is 20-200uL.
Step 4) contrast with color scale card, in liquid to be measured urea content for add urase group and do not add urase group with the difference of colour atla respective value, do not add urase group and color scale card contrasts the content that the numerical value * 0.5 obtained is NH4-N in liquid to be measured.
Above to theme disposal route and the device of a kind of resource provided by the present invention, be described in detail, apply specific case herein to set forth principle of the present invention and embodiment, the explanation of above embodiment just understands method of the present invention and core concept thereof for helping; Meanwhile, for one of ordinary skill in the art, according to thought of the present invention, all will change in specific embodiments and applications, in sum, this description should not be construed as limitation of the present invention.
What finally illustrate is, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (11)
1. the detection method of urea and total ammonia nitrogen in bean sprouts, is characterized in that: it, by detecting the ammonia nitrogen produced through urase decomposing urea, carries out sxemiquantitative according to ammonia-nitrogen content to urea, and its method detecting urea and total ammonia nitrogen comprises the following steps:
1) detecting thing is bean sprouts, is shredded bean sprouts, adds water and mixes, and room temperature is placed, and centre is shaken frequently, and obtaining supernatant is liquid to be measured;
2) get two parts respectively and detect liquid, portion adds urase, and another part adds isopyknic water, shakes up, room temperature catalysis;
3) to step 2) described in two parts of reactant liquors in add nessler reagent respectively, shake up;
4) step 3) shake up, observing response liquid color, contrasts with color scale card, to urea content sxemiquantitative.
2. the detection method of urea and total ammonia nitrogen in a kind of bean sprouts according to claim 1, is characterized in that: urase is beans urase; Described beans urase extracts from soya bean, green soya bean, black soya bean.
3. the detection method of urea and total ammonia nitrogen in a kind of bean sprouts according to claim 2, it is characterized in that: the preparation method of described beans urase is: in beans, add water, soaked overnight, add ethanol water making beating, centrifugal, supernatant is beans urase liquid, and supernatant vacuum freeze drying is obtained beans urase.
4. the detection method of urea and total ammonia nitrogen in a kind of bean sprouts according to claim 1, is characterized in that: step 1) ratio of bean sprouts and water is 1:1-1:10.
5. the detection method of urea and total ammonia nitrogen in a kind of bean sprouts according to claim 1, is characterized in that: step 1) room temperature standing time is 2-15min.
6. the detection method of urea and total ammonia nitrogen in a kind of bean sprouts according to claim 1, is characterized in that: step 2) sample and urase amount ratio be 0.5mL:20-200uL.
7. the detection method of urea and total ammonia nitrogen in a kind of bean sprouts according to claim 1, is characterized in that: step 2) urease activity is 30 ~ 180UmL
-1.
8. the detection method of urea and total ammonia nitrogen in a kind of bean sprouts according to claim 1, is characterized in that: step 2) urase catalysis time is 2-10min.
9. the detection method of urea and total ammonia nitrogen in a kind of bean sprouts according to claim 1, is characterized in that: step 3) consumption of developer is 20-200uL.
10. the detection method of urea and total ammonia nitrogen in a kind of bean sprouts according to claim 1, is characterized in that: step 4) shake up after, contrasts with color scale card in 0-10min.
The detection method of urea and total ammonia nitrogen in 11. a kind of bean sprouts according to claim 1, it is characterized in that: step 4) contrast with color scale card, in liquid to be measured urea content for add urase group with do not add urase group with the difference of colour atla respective value, NH in liquid to be measured
4the content of-N is do not add urase group and color scale card contrasts the numerical value * 0.5 obtained.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106018360A (en) * | 2016-05-13 | 2016-10-12 | 深圳职业技术学院 | Method for detecting urea based on metal organic framework material fluorescent sensor |
| CN106501248A (en) * | 2016-11-06 | 2017-03-15 | 东北师范大学 | A kind of method of urea in high-throughput enzyme sensor and detection human urine |
| CN112858276A (en) * | 2021-01-25 | 2021-05-28 | 郑州欧柯奇仪器制造有限公司 | Soybean milk maturity rapid test card and detection method thereof |
| CN119286984A (en) * | 2024-12-10 | 2025-01-10 | 浙江陆恒环境科技有限公司 | Water sample pretreatment reagent for total nitrogen detection in water, total nitrogen detection kit and detection method |
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