CN105053064B - A kind of harmless boilogical composite pesticide and preparation method thereof - Google Patents

Abstract

The invention belongs to the field of biological pesticides, and mainly discloses a pollution-free biological compound pesticide and a preparation method thereof. Shell, Sophora flavescens, Bacillus subtilis, Bacillus licheniformis, Streptomyces chamotis and Streptomyces aureus. The present invention not only greatly improves the bacteriostasis effect of biological pesticides by adding Chinese herbal medicine extracts such as Hyacinth hyacinthus and Rhizoma Sinensis to the pesticide, but also the Chinese herbal medicine extract has no poisonous effect on humans and animals, so that there is no need to worry about causing harm to humans and animals when the present invention is used. adverse effects. The biopesticide of the present invention has the characteristics of high efficiency, environmental protection, no secondary toxicity, not easy to produce drug resistance, no residue, no pollution, maintenance of ecological balance, and no pollution. It is an important guarantee for realizing green ecological agriculture and green enterprises. Broad prospects.

Description

A kind of pollution-free biological compound pesticide and its preparation method

technical field

The invention belongs to the field of biological pesticides, and mainly relates to a pollution-free biological compound pesticide and a preparation method thereof.

Background technique

There are mainly agricultural control methods, chemical control methods and biological control methods in the prevention and control of common flower diseases and insect pests. At present, the more traditional agricultural control plus chemical control mode is mainly adopted, and chemical pesticides are generally used, and pests and diseases are cut off branches and leaves. However, the long-term and large-scale application of chemical pesticides can easily cause serious consequences, such as the development of drug resistance, the destruction of ecological balance, and environmental pollution. Especially for ornamental flowers, tourists are close to them, and their ornamental properties and pesticide hazards coexist, threatening the health of tourists. At the same time, the increase in drug-resistant populations of pests and diseases also disrupts the balance of the natural ecosystem. Therefore, along with the development of agricultural production, people yearn for green consumption, and urgently hope to have a kind of biological pesticide that is pollution-free, broad-spectrum, and wide-ranging comprehensive control of diseases and insect pests, so as to meet the needs of the market and people.

Contents of the invention

In order to solve the above-mentioned technical problems, the present invention provides a pollution-free biological compound pesticide and its preparation method. The pesticide has remarkable antibacterial effect, is not harmful to humans and animals, and has low production cost.

The technical solution adopted in the present invention is: a pollution-free biological compound pesticide, the biological compound pesticide is made of the following raw materials in parts by weight: 5-8 parts of water hyacinth, 2-4 parts of rhizome, geranium 1-3 parts, 8-12 parts of forsythia, 7-13 parts of skullcap, 10-15 parts of gallnut, 5-7 parts of betel nut, 15-23 parts of coconut shell, 4-9 parts of Sophora flavescens, 0.2-0.5 parts of Bacillus subtilis 0.3-0.5 parts of Bacillus licheniformis, 0.1-0.3 parts of Streptomyces chamotis and 0.2-0.4 parts of Streptomyces aureus.

The bacillus subtilis is bacillus subtilis HS5B5.

The preparation method of described pollution-free biological compound pesticide comprises the following steps:

Step 1, take each raw material according to the parts by weight of the above-mentioned components, and set aside;

Step 2, putting Streptomyces chachis and Streptomyces aureus into the culture medium for cultivation, adding Bacillus subtilis and Bacillus licheniformis after 6-7 days, cultivating for 3 days to obtain the mixed bacterial agent for subsequent use;

Step 3: first crush the water hyacinth to 100 mesh, then add water to soak for 10-30 hours, heat it in a rotary evaporator after soaking, collect the distillate to obtain extract I, and set aside; forsythia and flavescens After mixing, grind to 100 mesh, then add to boiling water, filter after boiling for 40 minutes, concentrate the filtrate under reduced pressure to obtain extract II, and set aside; crush Scutellaria baicalensis to 100 mesh, add to boiling water and boil for 30 minutes, filter after boiling, Obtain the filtrate, then add sodium hydroxide with a mass percentage of 40% to the filtrate, adjust the pH to 6.5-7, and obtain a mixed solution for subsequent use; add an equal volume of ethanol with a volume percentage of 95% to the mixed solution, and stir evenly Filtrate with suction, then heat the obtained filtrate to 50°C, and adjust the pH to 1-2 with hydrochloric acid with a concentration of 2mol/L, wait for the precipitation to separate out, filter, collect the solid, and obtain the extract III for later use; Grass, gallnuts, betel nuts and coconut shells are mixed and crushed to 300 mesh to obtain mixed powder, which is set aside;

Step 4: firstly mix the extract I, extract II, extract III obtained in step 3 with the mixed powder, then add the mixed bacterial agent obtained in step 2, and stir evenly to complete the preparation of the pollution-free biological compound pesticide.

The Bacillus subtilis ( Bacillus subtilis ) HS5B5 in the present invention is preserved in the China General Microorganism Culture Collection and Management Center, and the preservation number is: CGMCC No. 6088. Bacillus licheniformis ( Bacillus licheniformis ) is preserved in the China General Microorganism Culture Collection and Management Center, and the preservation number is CGMCC 1.521. Streptomyces rimosus is preserved in the China General Microorganism Culture Collection Management Center with the preservation number CGMCC4.748. Streptomyces aureus is preserved in the China General Microorganism Culture Collection Management Center with the preservation number CGMCC 4.6322. Except for the laboratory preservation of Bacillus subtilis HS5B5, the above strains were purchased from Shandong Shenhai Biotechnology Co., Ltd.

Compared with the prior art, the present invention has at least the following advantages and beneficial effects:

1. The present invention not only greatly improves the bacteriostasis effect of biopesticides by adding Chinese herbal extracts such as Hyacinth hyacinth and Rhizoma Sinensis to pesticides, but also Chinese herbal extracts have no toxic effect on humans and animals, so there is no need to worry about the use of the present invention Adverse effects on humans and animals. The biocomposite pesticide of the present invention is suitable for the prevention and control of diseases and insect pests of common flowers, such as rose powdery mildew, rose black spot, peony rust, rose scale insect disease, peony brown spot, peony gray mold, peony root-knot nematode, etc., and has high efficiency. Non-toxic, environmentally friendly, no secondary toxicity, not easy to produce drug resistance, no residue, no pollution, maintain ecological balance, and no pollution. It is an important guarantee for the realization of green ecological agriculture and green enterprises, and has broad prospects.

2. In the present invention, by first cultivating Streptomyces fissures and Streptomyces aureus for 6-7 days and then adding two kinds of bacillus, not only the number of microorganisms can be increased, but also the bacteriostatic substances in biological pesticides can be increased. The present invention adds Streptomyces fissures and Streptomyces aureus to the biopesticide, which can effectively enhance the effect of the present invention on preventing and controlling scab, powdery mildew, nematode and other diseases and insect pests.

3. The water hyacinth in the present invention is soaked, distilled, etc. to obtain the active ingredient 2,6-dimethoxybenzoquinone, which has significant antibacterial and antiviral effects. The forsythin substances in Forsythia and matrine in Sophora flavescens cooperate with each other, have bactericidal activity, can kill bacteria and insects in a broad spectrum, have contact and stomach poisoning effects, and have obvious control effects on various flower diseases and insect pests. Baicalin, a flavonoid compound in Scutellaria baicalensis, has strong bactericidal activity against Gram-positive bacteria and negative bacteria, and does not produce drug resistance. The tannins in Guanzhong, geranium, gallnut, betel nut and coconut shell can bind to the surface proteins of bacteria to inactivate them, and then kill the bacteria. The combination of five Chinese herbal medicines has higher tannin content and the effect is more significant.

detailed description

A pollution-free biological compound pesticide, the biological compound pesticide is made of the following raw materials in parts by weight: 5-8 parts of water hyacinth, 2-4 parts of rhizome, 1-3 parts of geranium, 8- 12 parts, 7-13 parts of Scutellaria baicalensis, 10-15 parts of gallnut, 5-7 parts of betel nut, 15-23 parts of coconut shell, 4-9 parts of Sophora flavescens, 0.2-0.5 parts of Bacillus subtilis, 0.3-0.5 parts of Bacillus licheniformis , 0.1-0.3 parts of Streptomyces chamatis and 0.2-0.4 parts of Streptomyces aureus; said Bacillus subtilis is Bacillus subtilis HS5B5.

The preparation method of described pollution-free biological compound pesticide comprises the following steps:

Step 1, take each raw material according to the parts by weight of the above-mentioned components, and set aside;

Step 2, putting the Streptomyces chamobilis powder and the Streptomyces aureus powder into the culture medium for cultivation, adding Bacillus subtilis powder and Bacillus licheniformis powder after 6-7 days, cultivating for 3 days to obtain a mixed bacterial agent for subsequent use;

Step 3: first crush the water hyacinth to 100 mesh, then add water to soak for 10-30 hours, heat it in a rotary evaporator after soaking, collect the distillate to obtain extract I, and set aside; forsythia and flavescens After mixing, grind to 100 mesh, then add to boiling water, filter after boiling for 40 minutes, concentrate the filtrate under reduced pressure to obtain extract II, and set aside; crush Scutellaria baicalensis to 100 mesh, add to boiling water and boil for 30 minutes, filter after boiling, Obtain the filtrate, then add sodium hydroxide with a mass percentage of 40% to the filtrate, adjust the pH to 6.5-7, and obtain a mixed solution for subsequent use; add an equal volume of ethanol with a volume percentage of 95% to the mixed solution, and stir evenly Filtrate with suction, then heat the obtained filtrate to 50°C, and adjust the pH to 1-2 with hydrochloric acid with a concentration of 2mol/L, wait for the precipitation to separate out, filter, collect the solid, and obtain the extract III for later use; Grass, gallnuts, betel nuts and coconut shells are mixed and crushed to 300 mesh to obtain mixed powder, which is set aside;

Step 4: firstly mix the extract I, extract II, extract III obtained in step 3 with the mixed powder, then add the mixed bacterial agent obtained in step 2, and stir evenly to complete the preparation of the pollution-free biological compound pesticide.

In order to make the present invention have a better implementation effect, the medium described in step 2 is composed of potatoes, bran, brown sugar and water, wherein the parts by weight of each component are respectively: 19-21 parts of potatoes, 15-21 parts of bran 20 parts, brown sugar 2-3 parts and water 100 parts.

In order to make the content of the present invention more obvious and understandable, the present invention will be described in detail below in conjunction with specific examples.

Example 1:

A pollution-free biological compound pesticide, the biological compound pesticide is made of the following raw materials in parts by weight: 5 parts of water hyacinth, 2 parts of rhizome, 1 part of geranium, 8 parts of forsythia, 7 parts of skullcap, gallnut 10 parts, 5 parts of betel nut, 15 parts of coconut shells, 4 parts of Sophora flavescens, 0.2 part of Bacillus subtilis, 0.3 part of Bacillus licheniformis, 0.1 part of Streptomyces fissures and 0.2 part of Streptomyces aureus; described Bacillus subtilis is Bacillus subtilis HS5B5.

The preparation method of described pollution-free biological compound pesticide comprises the following steps:

Step 1, take each raw material according to the parts by weight of the above-mentioned components, and set aside;

Step 2, put the Streptomyces chamobilis powder and the Streptomyces aureus powder into the culture medium for cultivation, add Bacillus subtilis powder and Bacillus licheniformis powder after 6 days, and obtain the mixed bacterial agent after cultivating for 3 days, and set aside;

Step 3: first crush the water hyacinth to 100 mesh, then add water to soak for 10 hours, heat it in a rotary evaporator after soaking, collect the distillate to obtain extract I, and set aside; mix forsythia and flavescens Crushed to 100 mesh, then added to boiling water, filtered after boiling for 40 minutes, the filtrate was concentrated under reduced pressure to obtain extract II, and set aside; Scutellaria baicalensis was crushed to 100 mesh, added to boiling water and boiled for 30 minutes, filtered after boiling to obtain the filtrate , then adding mass percent to the filtrate is 40% sodium hydroxide, adjusting the pH to 6.5 to obtain the mixed solution, for subsequent use; adding an equal volume of ethanol with a volume percentage of 95% to the mixed solution, stirring evenly, and then Heat the obtained filtrate to 50°C, adjust the pH to 1 with hydrochloric acid with a concentration of 2 mol/L, wait for precipitation, filter, collect the solid to obtain extract III, and set aside; Coconut shells are mixed and crushed to 300 mesh to obtain mixed powder, which is set aside;

Step 4: firstly mix the extract I, extract II, extract III obtained in step 3 with the mixed powder, then add the mixed bacterial agent obtained in step 2, and stir evenly to complete the preparation of the pollution-free biological compound pesticide.

Example 2:

A pollution-free biological compound pesticide, the biological compound pesticide is made of the following raw materials in parts by weight: 8 parts of water hyacinth, 4 parts of rhizome, 3 parts of geranium, 12 parts of forsythia, 13 parts of skullcap, gallnut 15 parts, 7 parts of betel nut, 23 parts of coconut shells, 9 parts of Sophora flavescens, 0.5 part of Bacillus subtilis, 0.5 part of Bacillus licheniformis, 0.3 part of Streptomyces fissures and 0.4 part of Streptomyces aureus; described Bacillus subtilis is Bacillus subtilis HS5B5.

The preparation method of described pollution-free biological compound pesticide comprises the following steps:

Step 1, take each raw material according to the parts by weight of the above-mentioned components, and set aside;

Step 2, putting the Streptomyces chamobilis powder and the Streptomyces aureus powder into the medium for cultivation, adding the Bacillus subtilis powder and the Bacillus licheniformis powder after 7 days, and cultivating for 3 days to obtain a mixed bacterial agent for subsequent use;

Step 3: first crush the water hyacinth to 100 mesh, then add water to soak for 30 hours, heat it in a rotary evaporator after soaking, collect the distillate to obtain extract I, and set aside; mix forsythia and flavescens Crushed to 100 mesh, then added to boiling water, filtered after boiling for 40 minutes, the filtrate was concentrated under reduced pressure to obtain extract II, and set aside; Scutellaria baicalensis was crushed to 100 mesh, added to boiling water and boiled for 30 minutes, filtered after boiling to obtain the filtrate , then adding mass percent to the filtrate is 40% sodium hydroxide, adjusting the pH to 7, to obtain the mixed solution, for subsequent use; adding an equal volume of ethanol with a volume percentage of 95% to the mixed solution, stirring evenly, then suction filtration, and then Heat the obtained filtrate to 50°C, and adjust the pH to 2 with hydrochloric acid with a concentration of 2 mol/L, wait for precipitation, filter, collect the solid, and obtain the extract III, which is set aside; Coconut shells are mixed and crushed to 300 mesh to obtain mixed powder, which is set aside;

Step 4: firstly mix the extract I, extract II, extract III obtained in step 3 with the mixed powder, then add the mixed bacterial agent obtained in step 2, and stir evenly to complete the preparation of the pollution-free biological compound pesticide.

Example 3:

A pollution-free biological compound pesticide, the biological compound pesticide is made of the following raw materials in parts by weight: 7 parts of water hyacinth, 3 parts of rhizome, 2 parts of geranium, 10 parts of forsythia, 10 parts of scutellaria, gallnut 13 parts, 6 parts of betel nut, 19 parts of coconut shells, 7 parts of Sophora flavescens, 0.3 part of Bacillus subtilis, 0.4 part of Bacillus licheniformis, 0.2 part of Streptomyces fissures and 0.3 part of Streptomyces aureus; described Bacillus subtilis is Bacillus subtilis HS5B5.

The preparation method of described pollution-free biological compound pesticide comprises the following steps:

Step 1, take each raw material according to the parts by weight of the above-mentioned components, and set aside;

Step 2, putting the Streptomyces chamobilis powder and the Streptomyces aureus powder into the medium for cultivation, adding the Bacillus subtilis powder and the Bacillus licheniformis powder after 7 days, and cultivating for 3 days to obtain a mixed bacterial agent for subsequent use;

Step 3: first crush the water hyacinth to 100 mesh, then soak in water for 20 hours, heat it in a rotary evaporator after soaking, collect the distillate to obtain extract I, and set aside; mix forsythia and flavescens Crushed to 100 mesh, then added to boiling water, filtered after boiling for 40 minutes, the filtrate was concentrated under reduced pressure to obtain extract II, and set aside; Scutellaria baicalensis was crushed to 100 mesh, added to boiling water and boiled for 30 minutes, filtered after boiling to obtain the filtrate , then adding mass percent to the filtrate is 40% sodium hydroxide, adjusting the pH to 6.5 to obtain the mixed solution, for subsequent use; adding an equal volume of ethanol with a volume percentage of 95% to the mixed solution, stirring evenly, and then Heat the obtained filtrate to 50°C, and adjust the pH to 1.5 with hydrochloric acid with a concentration of 2 mol/L, wait for precipitation, filter, collect the solid, and obtain the extract III, which is set aside; Coconut shells are mixed and crushed to 300 mesh to obtain mixed powder, which is set aside;

Step 4: firstly mix the extract I, extract II, extract III obtained in step 3 with the mixed powder, then add the mixed bacterial agent obtained in step 2, and stir evenly to complete the preparation of the pollution-free biological compound pesticide.

Experimental Research Data

test one

On May 13, 2015, in Luoyang International Peony Garden, a sample plot planted with peonies was selected as the test object, and the plot was divided into three groups, A, B, and C, and grown in isolation.

Group A uses the present invention, and every mu land sprays 40mL, mixes clear water 30kg. Group B used the components after removing the Chinese herbal medicine extract in the present invention, sprayed 40mL per mu of land, and mixed with 30kg of clear water. Group C did not use the present invention, and sprayed 30kg of clear water.

Test results: One month later, the peony plants in group A were tall and vigorous, and no pests and diseases occurred; the peony plants in group B were not as tall as group A, but some pests and diseases occurred; the peonies in group C grew weakly, and there were obvious pests and diseases.

Test two

On May 26, 2015, in Luolong District, Luoyang City, a sample plot planted with Chinese rose was selected as the test object, and the plot was divided into three groups, A, B, and C, and grown in isolation.

Group A uses the present invention, and every mu land sprays 40mL, mixes clear water 30kg. Group B used the components after removing the Chinese herbal medicine extract in the present invention, sprayed 40mL per mu of land, and mixed with 30kg of clear water. Group C did not use the present invention, and sprayed 30kg of clear water.

Test results: One month later, the rose plants in group A grew vigorously, and no diseases and insect pests occurred; the rose plants in group B were not as tall as group A, but some diseases and insect pests occurred; the rose plants in group C grew weakly, and obvious diseases and insect pests occurred.

Claims (1)

1. a preparation method of pollution-free biological compound pesticide, it is characterized in that: comprise the following steps: Step 1, get 5-8 parts of water hyacinth, 2-4 parts of Rhizome, 1-3 parts of geranium, 8-12 parts of forsythia, 7-13 parts of Scutellaria baicalensis, 10-15 parts of Galla chinensis, 5-7 parts of betel nut, 15-23 parts of coconut shell, 4-9 parts of Sophora flavescens, 0.2-0.5 parts of Bacillus subtilis, 0.3-0.5 parts of Bacillus licheniformis, 0.1-0.3 parts of Streptomyces chamomile and 0.2 parts of Streptomyces aureus -0.4 parts, standby, wherein, Bacillus subtilis is Bacillus subtilis HS5B5; Step 2, putting Streptomyces chachis and Streptomyces aureus into the culture medium for cultivation, adding Bacillus subtilis and Bacillus licheniformis after 6-7 days, cultivating for 3 days to obtain the mixed bacterial agent for subsequent use; Step 3: first crush the water hyacinth to 100 mesh, then add water to soak for 10-30 hours, heat it in a rotary evaporator after soaking, collect the distillate to obtain extract I, and set aside; forsythia and flavescens After mixing, grind to 100 mesh, then add to boiling water, filter after boiling for 40 minutes, concentrate the filtrate under reduced pressure to obtain extract II, and set aside; crush Scutellaria baicalensis to 100 mesh, add to boiling water and boil for 30 minutes, filter after boiling, Obtain the filtrate, then add sodium hydroxide with a mass percentage of 40% to the filtrate, adjust the pH to 6.5-7, and obtain a mixed solution for subsequent use; add an equal volume of ethanol with a volume percentage of 95% to the mixed solution, and stir evenly Filtrate with suction, then heat the obtained filtrate to 50°C, and adjust the pH to 1-2 with hydrochloric acid with a concentration of 2mol/L, wait for the precipitation to separate out, filter, collect the solid, and obtain the extract III for later use; Grass, gallnuts, betel nuts and coconut shells are mixed and crushed to 300 mesh to obtain mixed powder, which is set aside; Step 4: firstly mix the extract I, extract II, extract III obtained in step 3 with the mixed powder, then add the mixed bacterial agent obtained in step 2, and stir evenly to complete the preparation of the pollution-free biological compound pesticide.