CN105010380B - Bactericidal composition containing three octyl benzene sulfonate of biguanides and chitosan - Google Patents

Abstract

The invention discloses a bactericidal composition containing biguanide trioctylbenzene sulfonate and chitosan, the active ingredients are biguanide trioctylbenzene sulfonate and chitosan, and biguanide trioctylbenzene The mass ratio of sulfonate and chitosan is 40:1 to 1:10, and the weight percentage of the sum of biguanide trioctylbenzenesulfonate and chitosan in the bactericidal composition is 0.05%- 90%; the bactericidal composition of the present invention is suitable for preventing and treating wheat powdery mildew, strawberry powdery mildew, citrus blue mold, citrus acid rot, citrus anthracnose, cucumber downy mildew, rice sheath blight, rice blast, potato early blight , potato late blight, and watermelon anthracnose; the present invention reduces the dosage of pesticides, reduces the impact of pesticides on the environment, reduces the cost of medication, can delay or overcome the generation of disease resistance, prolongs the service life of medicaments, and is beneficial to diseases comprehensive management.

Description

Bactericidal composition containing biguanide trioctylbenzenesulfonate and chitosan

technical field

The fungicidal composition involved in the present invention uses biguanide trioctylbenzenesulfonate and chitosan as active ingredients, and is suitable for preventing and treating wheat powdery mildew, strawberry powdery mildew, citrus penicillium, citrus acid rot, citrus Anthracnose, cucumber downy mildew, rice sheath blight, rice blast, potato early blight, potato late blight, watermelon anthracnose.

Background technique

Biguanide trioctyl benzene sulfonate, English common name Iminoctadine tris (albesilate), also known as Baikede, mainly acts on the synthesis of acid-like compounds of pathogenic bacteria and cell membrane function, inhibits spore germination, germ tube elongation, and attachment and the formation of mycelia, strong local penetration, it is a contact and preventive fungicide; biguanide trioctylbenzene sulfonate has a wide bactericidal spectrum, and is used to prevent and treat diseases caused by Ascomycetes and Deuteromycetes. Diseases that occur during storage also have a good control effect, and can make the fruit surface bright and increase the value of commodities.

Chitosan, the common name in English Chitosan, is a water-soluble product formed after deacetylation of chitin by concentrated alkaline water. The role of crop disease resistance and immune function; chitosan has multiple uses such as health care, medicine, and agriculture, and can be used for cucumber downy mildew, powdery mildew, tomato late blight, viral diseases, apple spot leaf spot, citrus (fruit) anthracnose It has a broad bactericidal spectrum against crop diseases such as rice blast and rice blast.

Through experiments, it was found that biguanide trioctylbenzene sulfonate mixed with chitosan can be used in combination with Colletotrichum, Penicilium italicum, Penicilium digitatum, Geotrichum, Alternaria, Phyricularia, Phytophthora, Pseudoperonospora, Plasmopara, Rhizoctonia, Botrytis ( Fungal plant pathogens such as Botrytis, Fusarium, Fusicladium, Sphlocaea and Erysiphales have synergistic effects, especially It is effective against Colletotrichum, Penicilium italicum, Geotrichum, Phyricularia, Phytophthora, streptococci within the ratio of 20:1 to 1:20. Pathogens of Alternaria and Erysiphe had obvious synergistic effect.

The composition of biguanide trioctylbenzene sulfonate and chitosan can be shaped into a commonly used dosage form in agriculture through certain technical means.

Contents of the invention

The invention aims to provide a synergistic bactericidal composition whose active ingredients are biguanide trioctylbenzenesulfonate and chitosan, and provide an effective management tool for the prevention and control of crop fungal diseases.

The technical solution adopted in the present invention is: a bactericidal composition containing biguanide trioctylbenzenesulfonate and chitosan, the active ingredients are biguanide trioctylbenzenesulfonate and chitosan, biguanide The mass ratio of trioctylbenzenesulfonate and chitosan is 40:1 to 1:10, the weight of the sum of the mass of biguanide trioctylbenzenesulfonate and chitosan in the bactericidal composition The percentage is 0.05%-90%.

Further, the fungicidal composition can be formulated into application forms suitable for agricultural use such as suspension concentrate, oil suspension concentrate, water dispersible granule, wettable powder, etc. according to methods known to those skilled in the art.

The bactericidal composition also contains common auxiliary agents required for the preparation of pesticide preparations. Common auxiliary agents are solvents, emulsifiers, wetting agents, stabilizers, dispersants, thickeners, pH regulators, defoamers, antifreeze One or more mixtures of additives, fillers, etc. are known substances and are various auxiliary agents commonly used in pesticide preparations. They can vary according to different situations and are not particularly limited.

The fungicidal composition provided by the invention is suitable for preventing and treating wheat powdery mildew, strawberry powdery mildew, citrus blue mold, citrus acid rot, citrus anthracnose, cucumber downy mildew, rice sheath blight, rice blast, and potato early blight , potato late blight, watermelon anthracnose and other crop diseases.

The fungicidal composition provided by the present invention is usually used by spraying, and other agricultural application techniques can also be used as required.

The bactericidal composition provided by the invention has the following beneficial effects:

1. The bactericidal composition provided by the present invention shows obvious synergistic effect within a certain proportion range, and the control effect of the composition is significantly improved compared with a single agent, which reduces the dosage of pesticides and reduces the impact of pesticides on the environment. impact, reducing the cost of medication.

2. The active ingredients of the fungicidal composition provided by the present invention have different action mechanisms. The application of the composition can delay or overcome the occurrence of disease resistance, prolong the service life of the agent, and be beneficial to the comprehensive management of diseases.

Detailed ways

In order to better understand the essence of the present invention, the following examples are used for further illustration, but the present invention is not limited to these examples. In these examples, all percentages are by weight unless otherwise indicated.

Example 1: Indoor toxicity test of biguanide trioctylbenzenesulfonate and chitosan to Blumeria graminis f.sp.tritici.

The test object is the wheat powdery mildew pathogen Blumeria graminis f.sp. tritici collected from the field, and the pot test method is used. According to Sun Yunpei's method, the synergistic effect of drug mixture is evaluated according to the co-toxicity coefficient (CTC), that is, CTC≤80 is synergistic antagonism, 80<CTC<120 is synergistic antagonism, and CTC≥120 is synergistic effect. Actual toxicity index (ATI) = (EC ₅₀ of standard drug / EC ₅₀ of test drug) × 100; theoretical toxicity index TTI = toxicity index of drug A × percentage of drug A in the mixture + drug B The toxicity index of the mixture × the percentage content of agent B in the mixture; the co-toxicity coefficient (CTC) = [the measured toxicity index of the mixture (ATI) / the theoretical toxicity index of the mixture (TTI)] × 100.

Table 1. Indoor toxicity test results of biguanide trioctylbenzenesulfonate and chitosan on Blumeria graminis f.sp.tritici.

It can be seen from Table 1 that the mixed mass ratio of biguanide trioctylbenzenesulfonate to chitosan is between 40:1 and 1:10, which is effective against Blumeria graminis f.sp. .tritici) has obvious synergistic effect, and the co-toxicity coefficients are all above 150.

Example 2: Indoor toxicity test of biguanide trioctylbenzenesulfonate and chitosan to Penicillium italicum.

The test object is Penicillium italicum, the pathogen of Penicillium italicum collected from citrus fruit, and the mycelium growth method.

Table 2. Indoor toxicity test results of biguanide trioctylbenzenesulfonate and chitosan to Penicillium italicum.

It can be seen from Table 2 that the mixed mass ratio of biguanide trioctylbenzene sulfonate to chitosan is between 40:1 and 1:10, which has obvious synergistic effect on Penicillium italicum and co-toxicity The coefficients are all above 150.

Example 3: Indoor toxicity test of biguanide trioctylbenzenesulfonate·chitosan to Phytophthora infestans.

The test object is the potato late blight pathogen Phytophthora infestans (Phytophthora infestans) collected from the field, and mycelium growth method.

Table 3. Indoor toxicity test results of biguanide trioctylbenzenesulfonate and chitosan to Phytophthora infestans.

It can be seen from Table 3 that the mixed mass ratio of biguanide trioctylbenzenesulfonate to chitosan is between 40:1 and 1:10, which has obvious synergistic effect on Phytophthora infestans, The co-toxicity coefficients were all above 150.

Example 4: Indoor toxicity determination of biguanide trioctylbenzenesulfonate·chitosan against Rhizoctonia solani.

The test object is Rhizoctonia solani (Rhizoctonia solani), the pathogen of rice sheath blight collected from the field, using the mycelial growth method.

Table 4. Indoor toxicity test results of biguanide trioctylbenzenesulfonate and chitosan against Rhizoctonia solani.

It can be seen from Table 4 that the mixed mass ratio of biguanide trioctylbenzenesulfonate and chitosan is between 40:1 and 1:10, which has obvious synergistic effect on Rhizoctonia solani. The co-toxicity coefficients were all above 140.

Example 5: Indoor toxicity test of biguanide trioctylbenzenesulfonate and chitosan to Phyricularia grisea.

The test object is the rice blast pathogen Phyricularia grisea collected from the field, and the mycelium growth method is used.

Table 5. Laboratory toxicity test results of biguanide trioctylbenzenesulfonate and chitosan to Phyricularia grisea.

It can be seen from Table 5 that the mixed mass ratio of biguanide trioctylbenzenesulfonate to chitosan is between 40:1 and 1:10, and it has obvious synergistic effect on Phyricularia grisea. The toxicity coefficients are above 140.

Example 6: Indoor toxicity test of biguanide trioctylbenzenesulfonate and chitosan to Colletotrichum gloeosporioides.

The test object is the watermelon anthracnose pathogen colletotrichum gloeosporioides collected from the field, and the mycelium growth method is used.

Table 6. Indoor toxicity test results of biguanide trioctylbenzenesulfonate·chitosan against Colletotrichum gloeosporioides.

It can be seen from Table 6 that the mixture of biguanide trioctylbenzenesulfonate and chitosan between 40:1 and 1:10 has obvious synergistic effect on Colletotrichum gloeosporioides, and the co-toxicity coefficient Both are above 140.

Example 7: Indoor toxicity test of biguanide trioctylbenzenesulfonate·chitosan to Alternaria solani.

The test object was Alternaria solani, the causative agent of potato early blight collected from the field, using mycelium growth method.

Table 7. Indoor toxicity test results of biguanide trioctylbenzenesulfonate and chitosan to Alternaria solani (Alternaria solani).

It can be seen from Table 7 that the mixed mass ratio of biguanide trioctylbenzenesulfonate to chitosan is between 40:1 and 1:10, which has obvious synergistic effect on Alternaria solani, The co-toxicity coefficients were all above 150.

Example 8: Indoor toxicity test of biguanide trioctylbenzenesulfonate and chitosan to Geotrichum candidum.

The test object is Geotrichum candidum, which is the pathogen of citrus fruit sour rot, and the mycelium growth method

Table 8. Indoor toxicity test results of biguanide trioctylbenzenesulfonate·chitosan to Geotrichum candidum.

It can be seen from Table 8 that the mixed mass ratio of biguanide trioctylbenzenesulfonate to chitosan is between 40:1 and 1:10, which has obvious synergistic effect on Geotrichum candidum, and co-toxicity The coefficients are all above 140.

Below in conjunction with preparation embodiment the present invention is further described.

Example 9: (biguanide trioctylbenzenesulfonate chitosan suspension).

The above formula is pre-crushed according to the proportion, and then put into a sand mill for grinding, and after high-shear mixing, the biguanide trioctylbenzenesulfonate·chitosan suspension concentrate is prepared to obtain the corresponding weight percentage.

Example 10: 30% biguanide trioctylbenzenesulfonate·chitosan suspension concentrate.

Biguanide trioctylbenzenesulfonate 210g, chitosan 106g, sodium lignosulfonate 35g, fatty acid ester sodium sulfate 31g, p-methoxy fatty amidobenzenesulfonic acid 5.1g, fat 5.1 grams of alcohol polyoxyethylene ether, 5.2 grams of glycerin, 1.1 grams of xanthan gum, deionized water to make up to 1000 grams, the above formula is pre-crushed in proportion, then added to a sand mill for grinding, and mixed by high shear The biguanide trioctyl benzene sulfonate chitosan suspension was prepared after preparation. After testing, the biguanide trioctyl benzene sulfonate content was 20.2%, the chitosan content was 10.2%, and the total effective content was 40.4%.

Embodiment 11: Prevention and treatment of citrus fruit anthracnose.

40.4% biguanide trioctyl benzene sulfonate chitosan suspension concentrate is diluted with water at 100mg/kg and sprayed, the control effect is 97.2% and 92.3% 15 days and 30 days after the drug, 50% biguanide trioctyl Benzenesulfonate water dispersible granules were diluted with water at 100mg/kg and 10% chitosan suspension was sprayed at 100mg/kg, and the control effects were 75.1%, 60.2%, 87.4%, and 77.3% in 15 days and 30 days after the drug. %. The combination of biguanide trioctylbenzenesulfonate and chitosan has obvious synergistic effects, and the control effect on citrus fruit anthracnose is significantly better than that of a single agent, which reduces the cost of use and provides growers with a citrus A more effective management tool for fruit anthracnose.

Embodiment 12: For preventing and treating citrus fruit blue mold.

40.4% biguanide trioctyl benzene sulfonate chitosan suspension concentrate is diluted with water at 100 mg/kg and sprayed, and the control effect is 94.9% and 87.8% after 7 days and 14 days after the drug, and 50% biguanide trioctyl Besylate water dispersible granules were sprayed with 100mg/kg of 10% chitosan suspension and diluted with water at 100mg/kg, and the control effects were 81.0%, 70.2%, 80.3%, 71.5% in 15 days and 30 days after the drug. %. The combination of biguanide trioctylbenzenesulfonate and chitosan has obvious synergistic effect, and the control effect on citrus fruit blue mold is obviously better than that of a single agent, which reduces the cost of use and provides a kind of More effective management tools for Penicillium citrus fruit.

Example 13: (biguanide trioctylbenzenesulfonate chitosan oil suspension).

Put the above materials (except organic bentonite) into the reaction kettle mixer together, stir for 2 hours, open the feed valve at the bottom of the kettle, inject the materials into the multi-stage sand mill for grinding, and the fineness of the ground materials is 98% through 1- After 5um, the material is put into the high-shear emulsification kettle together with the pre-dissolved organic bentonite. After emulsification, the biguanide trioctylbenzenesulfonate with good flowability and corresponding weight percentage can be produced. Butan oil suspension.

Example 14: 30% biguanide trioctylbenzenesulfonate·chitosan oil suspension.

155 grams of biguanide trioctylbenzene sulfonate, 156 grams of chitosan, 45 grams of sodium lignosulfonate, 52 grams of fatty alcohol polyoxyethylene ether, 249 grams of methyl oleate, 83 grams of organic bentonite, ten Dialkyl sulfonate calcium salt 60 grams, turpentine to make up to 1000 grams, the above formula is pre-crushed in proportion, then added to a sand mill for grinding, and prepared after high-shear mixing to obtain biguanide trioctylbenzene sulfonic acid Salt Chitosan oil suspension concentrate, after testing, the effective content of biguanide trioctylbenzenesulfonate is 15.1%, the effective content of chitosan is 15.1%, and the total effective content is 30.2%.

Embodiment 15: For preventing and treating citrus fruit sour rot.

30.2% biguanide trioctyl benzene sulfonate chitosan oil suspension was diluted with water at 100 mg/kg and sprayed, the control effect was 97.4%, 92.1% and 50% biguanide trioctane 15 days and 30 days after the drug 100mg/kg of phenylsulfonate water dispersible granules, 10% chitosan suspension was diluted with water at 100mg/kg and sprayed, and the control effects were 86.6%, 77.2% and 83.6%, 15 days and 30 days after the drug, respectively. 72.8%. The combination of biguanide trioctylbenzenesulfonate and chitosan has obvious synergistic effects, and the control effect on citrus fruit acid rot is significantly better than that of a single agent, which reduces the cost of use and provides growers with a A more effective management tool for citrus fruit sour rot.

Embodiment 16: be used for preventing and treating oily cucumber downy mildew. 30.2% biguanide trioctyl benzene sulfonate Chitosan oil suspension was diluted with 50g a.i./ha and sprayed with water, and the control effect was 88.1%, 83.2% and 50% biguanide trioctane 7 days and 14 days after the drug Alkylbenzenesulfonate water-dispersible granules are diluted with water according to 50g a.i./ha and 10% chitosan suspension are sprayed with 50g a.i./ha, and the control effects are 65.2%, 52.4% and 74.3%, 65.6%. The combination of biguanide trioctylbenzene sulfonate and chitosan has obvious synergistic effect, and the control effect on cucumber downy mildew is significantly better than that of a single agent, which reduces the cost of use and provides growers with a cucumber A more effective management tool for downy mildew.

Example 17: (biguanide trioctylbenzenesulfonate·chitosan water dispersible granule).

Put the above materials together into the conical mixer and mix them evenly, and then crush them through the jet mill, and then mix the crushed materials through the conical mixer. Knead into a plastic material, and finally put the material into an extrusion granulator to extrude and granulate. After granulation, it is dried and sieved to obtain biguanide trioctylbenzenesulfonate chitin Polysaccharide water dispersible granules.

Example 18: 60% biguanide trioctylbenzenesulfonate·chitosan water dispersible granule.

455 grams of biguanide trioctylbenzene sulfonate, 155 grams of chitosan, 43 grams of sodium lignosulfonate, 61 grams of sodium polycarboxylate, 53 grams of glucose, 39 grams of tea dry powder, and kaolin to make up to 1000. The above formula was dry crushed, granulated, dried, and sieved to prepare biguanide trioctylbenzenesulfonate Chitosan water dispersible granules. After testing, biguanide trioctylbenzenesulfonate The effective content is 45.1%, the effective content of chitosan is 15.1%, and the total effective content is 60.2%.

Embodiment 19: For preventing and treating strawberry powdery mildew.

60.2% biguanide trioctyl benzene sulfonate Chitosan water dispersible granules are diluted with water at 50g a.i./ha and sprayed, and the control effect is 86.2%, 80.1% and 50% biguanide trioctane 7 days and 14 days after the drug Alkylbenzenesulfonate water dispersible granules are diluted with water at 50ga.i./ha and 10% chitosan suspensions are sprayed with water at 50g a.i./ha. % and 75.6%, 66.6%. The combination of biguanide trioctylbenzenesulfonate and chitosan has obvious synergistic effect, and the control effect on strawberry powdery mildew is significantly better than that of a single agent, which reduces the cost of use and provides a strawberry white powder for growers. more effective disease management tools.

Embodiment 20: For preventing and treating potato black mole disease.

60.2% biguanide trioctyl benzene sulfonate chitosan water dispersible granules are diluted with water at 50g a.i./ha and sprayed, and the control effect is 89.3%, 81.4% and 50% biguanide trioctane 7 days and 14 days after the drug Alkylbenzenesulfonate water dispersible granules are diluted with water at 50ga.i./ha and 10% chitosan suspensions are sprayed at 50g a.i./ha. % and 77.6%, 67.3%. The combination of biguanide trioctylbenzenesulfonate and chitosan has obvious synergistic effects, and the control effect on potato black mole is significantly better than that of a single agent, which reduces the cost of use and provides farmers with a potato A more effective management tool for mole disease.

Example 21: (biguanide trioctylbenzenesulfonate Chitosan wettable powder).

Put the above materials together into the conical mixer and mix them evenly, then pulverize them through the jet mill, and then mix the pulverized materials through the conical mixer. The fineness of the mixed materials is 98% and pass through a 600-mesh standard sieve to obtain the corresponding weight Percentage of biguanide trioctylbenzenesulfonate Chitosan wettable powder.

Example 22: 50% biguanide trioctylbenzenesulfonate·chitosan wettable powder.

Biguanide Trioctylbenzenesulfonate 413g, Chitosan 109g, Calcium Lignosulfonate 51g, Sodium Lauryl Sulfate 44g, Sodium Butyl Naphthalene Sulfonate 52g, White Carbon Black 55g gram, kaolin supplemented to 1000 grams. The above formula is coarsely pulverized in proportion, put into the mixer and mixed evenly, and then the wettable powder of biguanide trioctylbenzene sulfonate chitosan can be obtained after airflow pulverization. After testing, biguanide trioctylbenzene The effective content of sulfonate is 40.2%, the effective content of chitosan is 10.1%, and the total effective content is 50.3%.

Embodiment 23: It is used for preventing and treating early blight of ornamental potato.

50.3% biguanide trioctyl benzene sulfonate chitosan wettable powder is diluted with 50g a.i./ha and sprayed with water, and the control effect is 86.6% and 78.9% after 7 days and 14 days of medicine, and 50% biguanide trioctyl Alkylbenzenesulfonate water dispersible granules are diluted with water at 50ga.i./ha and 10% chitosan suspension is sprayed with water at 50g a.i./ha. % and 75.1%, 66.4%. The combination of biguanide trioctylbenzenesulfonate and chitosan has obvious synergistic effects, and the control effect on potato early blight is significantly better than that of a single agent, which reduces the cost of use and provides farmers with a potato early blight more effective disease management tools.

Embodiment 24: For preventing and treating late blight of potato.

50.3% biguanide trioctyl benzene sulfonate chitosan wettable powder is diluted with water at 50g a.i./ha and sprayed, and the control effect is 87.5%, 80.2% and 50% biguanide trioctane 7 days and 14 days after the drug Alkylbenzenesulfonate water dispersible granules are diluted with water at 50ga.i./ha and 10% chitosan suspensions are sprayed at 50g a.i./ha. % and 76.7%, 70.3%. The combination of biguanide trioctylbenzenesulfonate and chitosan has obvious synergistic effects, and the control effect on potato late blight is significantly better than that of a single agent, which reduces the cost of use and provides farmers with a potato late blight More effective management tools for disease.

Claims (3)

1. A bactericidal composition containing biguanide trioctylbenzenesulfonate and chitosan, characterized in that: the active ingredients are biguanide trioctylbenzenesulfonate and chitosan, biguanide trioctane The mass fraction ratio of phenylbenzenesulfonate and chitosan is 40:1 to 1:10, and the weight percentage of the sum of the mass of biguanide trioctylbenzenesulfonate and chitosan in the bactericidal composition is 0.05 %-90%. 2. a kind of bactericidal composition containing biguanide trioctylbenzene sulfonate and chitosan according to claim 1, is characterized in that: the dosage form of described bactericidal composition is suspension concentrate, oil suspension concentrate, One of water dispersible granules and wettable powders. 3. a kind of bactericidal composition containing biguanide trioctylbenzene sulfonate and chitosan described in claim 1 is preventing and treating wheat powdery mildew, strawberry powdery mildew, citrus penicillium, citrus acid rot, citrus Anthracnose, cucumber downy mildew, rice sheath blight, rice blast, potato early blight, potato late blight, and watermelon anthracnose.