CN104996436B - A kind of bactericidal composition of containing fluazinam and tiadinil - Google Patents

Abstract

The invention discloses a bactericidal composition containing fluazinam and tiiazil. The mass ratio of the active components fluazinam to tiiazil is 15:1-1:15. The present invention has remarkable control effects on rice sheath blight and other crop fungal diseases, and the combined use of the two agents can reduce the dosage, effectively reduce ecological damage and environmental pollution, and improve crop yield and quality; Amylbactam is of low toxicity, safe for humans, animals, and beneficial organisms; it can delay the resistance of bacteria to a single agent. Fluazinam and thiazinil have different structural types and different mechanisms of action. Combining the two in a certain proportion has high bactericidal activity in actual use, and can avoid fluazinam and thiazide to a greater extent. The selection pressure on pathogens caused by the single agent of methamidide alone delays the emergence and development of drug resistance of pathogens, and reduces the total dosage per unit area, which is economical, efficient and environmentally friendly.

Description

A bactericidal composition containing fluazinam and thiazilamide

technical field

The invention belongs to the field of agricultural fungicides, and in particular relates to a fungicidal composition containing fluazinam and thiazilamide.

Background technique

Fluazinam (English common name: Fluazinam), the molecular formula is C13H4Cl2F6N4O4, and the structural formula is as follows:

The common chemical name is 3-chloro-N-(3-chloro-5-trifluoromethyl-2-pyridyl)-a,a,a-trifluoro-2,6-dinitro-p-toluidine, Pyridinamine derivatives, dinitroaniline fungicides. It has no therapeutic effect and systemic activity, and is a broad-spectrum and highly effective protective fungicide. Very effective against Alternaria, Phytophthora, Plasmodium, Sclerotinia and Scrubella. It also has a good effect on Botrytis cinerea resistant to benzimidazole and dicarboximide fungicides, and also has a good effect on rice damping-off disease caused by Rhizopus. This product is extremely resistant to rain erosion and has a long residual effect. In addition, it also has the function of controlling herbivorous mites. Since the β-trifluoromethylampyridine moiety plays a unique role in transporting compounds to the active sites of pathogenic bacteria, it can inhibit the germination, penetration, growth of hyphae and formation of spores of pathogenic bacteria during infection.

Tiadinil (English common name: Tiadinil Standard), the molecular formula is C ₁₁ H ₁₀ ClN ₃ OS, and the structural formula is as follows:

The common chemical name is 3'-chloro-4,4'-dimethyl-1,2,3-thiadiazole-5-carboxanilide, a novel systemic fungicide, which can be quickly absorbed by the root and transmitted to other parts . The drug itself has poor inhibitory activity against pathogens, and can induce the production of disease-resistant genes by preventing the hyphae of pathogens from invading adjacent healthy cells. It is suitable for water treatment, has a long duration, and has a good control effect on leaf blast and ear blast, especially suitable for rice seedling boxes. The effect of medication is especially good in the early stage of disease onset.

Contents of the invention

The object of the present invention is to provide a novel fungicide composition containing fluazinam and thiazil, which is used for preventing and treating various crop diseases such as rice sheath blight. Fluazinam and thiazilamide have different structure types and different mechanisms of action. Combining the two in a certain proportion has high bactericidal activity in actual use, and can avoid fluazinam and thiazide to a greater extent. The selective pressure on pathogens caused by the single agent of methamidide alone delays the emergence and development of drug resistance of pathogens, and reduces the total dosage per unit area, which is economical, efficient and environmentally friendly.

The bactericidal composition containing fluazinam and tiiazil of the present invention comprises the active components fluazinam and tiiazil in a mass ratio of 15:1 to 1:15.

Preferably, the mass ratio of fluazinam to tiiazil is 10:1-1:10, more preferably 5:1-1:5, and most preferably 1:1.

The composition of the present invention is added with commonly used agricultural fungicide auxiliaries to prepare a commonly used agricultural fungicide composition dosage form.

The dosage form of the bactericidal composition can be water suspension concentrate, wettable powder, water dispersible granule. The agricultural fungicide additives used include various additives such as wetting agents, dispersants, thickeners, defoamers, fillers, etc. The wetting agents and dispersants used can be alkylnaphthalene sulfonate formaldehyde condensates, Sodium Dibutylnaphthalene Sulfonate Formaldehyde Condensate, Sodium Oleic Acid Methylaminoethyl Sulfonate, Sodium Lignosulfonate, Calcium Lignosulfonate, Alkylphenol Polyoxyethylene Ether, Alkylphenol Polyoxyethylene One or more of phosphate ester, castor oil polyoxyethylene ether, etc.; the thickener used can be xanthan gum, polyvinyl alcohol, etc.; the defoamer used can be phosphate defoamer and silicone One or more of defoamers; the fillers used can be one or more of white carbon black, kaolin, attapulgite, etc.;

Preferably, the active ingredients composed of fluazinam and tiiazil account for 10% to 60% of the total mass of the fungicidal composition.

The fungicidal composition of the invention can be used for preventing and treating fungal diseases of rice sheath blight and other crops.

Through detailed analysis of the mechanism of action and bactericidal properties of fluazinam and thiazilamide, the Wadley method was used for experimental design and result processing. Firstly, measure the EC ₅₀ of two single compounds (hereinafter referred to as “drugs”) of fluazinam and tiiazinamide, and set the combination ratio of the two drugs according to the Wadley method according to the EC ₅₀ of the two single compounds, and determine according to the SR value of the Wadley synergy index. The appropriate composition ratio is then verified by Sun Yunpei's method.

The target of the test is rice sheath blight. The test uses the colony diameter method. Take the mother solution of fluazinam and thiazilamide, and configure it into a flat plate of the required drug solution, about 14ml per dish, and set a non-drug plate control (CK) at the same time, repeat 3 dishes for each treatment, that is, repeat 3 times. At the edge of the pre-cultured colony, use a sterilized hole puncher to prepare mycelium pieces with a diameter of 5mm, move them to different medium plates, culture at 28°C for 4 days, measure the diameter of the colony (mm), and calculate the growth inhibition rate by the following formula :

Convert the mycelial growth inhibition rate into the inhibition probability value (Y), convert the drug concentration into the concentration logarithm (X), and draw the toxicity regression line according to the concentration logarithm as the abscissa and the probability value as the ordinate, to obtain fluazinam Toxicity regression equation of single agent and thiazinil and its mixture against rice sheath blight bacteria, and calculate EC ₅₀ , EC ₉₀ value and correlation coefficient r value.

According to the Wadley method to evaluate the interaction of the mixture, the calculation formula is as follows:

EC _{50 (theoretical value)} = (a+b)/(a/EC _50a + b/EC _50b ), SR = EC _{50 (theoretical value)} /EC _{50 (actual value)} .

Where a and b are the content ratios of each component in the mixture, and the effect of the mixture is analyzed by SR value. SR ≤ 0.5, the two agents have an antagonistic effect; SR = 0.5 ~ 1.5, the two agents have an additive effect; SR ≥ 1.5, the two agents have a synergistic effect.

Table 1 The results of determination of the potency of fluazinam and tiiazil alone and their mixtures against rice sheath blight virus

From the data in Table 1, it can be concluded that when fluazinam and thiazilamide are mixed at a ratio of 15:1 to 1:15, the growth of rice sheath blight mycelium can be inhibited, and the rice sheath blight mycelium can be strongly inhibited. growth, in which fluazinam and thiazinil 5:1～1:5 showed synergistic effect. The 1:1 mixture of fluazinam and thiazilamide has the best effect on inhibiting rice sheath blight mycelia.

The present invention has good control effect on rice sheath blight and other crop fungal diseases, and the combined use of the two agents can reduce the dosage, effectively reduce ecological damage and environmental pollution, and can improve crop yield and quality; in the present invention, fluridine Both amine and thiazilamide are of low toxicity, safe for humans, animals, and beneficial organisms; they can delay the resistance of bacteria to a single agent.

detailed description

The following examples are a further description of the content of the present invention as an explanation of the technical content of the present invention, but the essential content of the present invention is not limited to the following examples, those of ordinary skill in the art can and should know any Simple changes or replacements of the essential spirit of the invention shall fall within the scope of protection required by the present invention.

Example 1

The preparation method of 60% fluazinam·thiazilamide suspension concentrate: by mass percentage.

Fluazinam 30%;

Thianodil 30%;

Alkyl naphthalene sulfonate formaldehyde condensate 4%;

Alkylphenol polyoxyethylene phosphate 3%;

Xanthan gum 0.2%;

Silicone defoamer 0.4%;

water balance.

Preparation method: According to the formula, weigh the above components in proportion, add to a ball mill and mill for 30 minutes, filter, pump into the dispersion tank for 3 minutes at high speed, and then fully grind with a sand mill to control the particle diameter of the solid component within 2 microns , and stir evenly after grinding to obtain a suspension product. 60-80g per mu is used to control rice sheath blight and the effect can reach more than 88%.

Example 2

The preparation method of 30% fluazinam·thiazilamide suspension concentrate: by mass percentage.

Fluazinam 15%;

Thianodil 15%;

Sodium lignosulfonate 4%;

Alkylphenol polyoxyethylene phosphate 3%;

Xanthan gum 0.2%;

Phosphate ester defoamer 0.3%;

water balance.

Preparation method, with embodiment 1. 60-80g per mu is used to control rice sheath blight and the effect can reach 86%.

Example 3

The preparation method of 20% fluazinam·thiazilamide suspension concentrate: by mass percentage.

Fluazinam 10%;

Thianodil 10%;

Sodium Dibutyl Naphthalene Sulfonate 5%;

Castor Oil Ethoxylate 4%;

Polyvinyl alcohol 0.2%;

Phosphate ester defoamer 0.4%;

water balance.

Preparation method, with embodiment 1. 60-80g per mu is used to control rice sheath blight and the effect can reach 83%.

Example 4

Preparation method of 40% fluazinam·thiazilamide wettable powder: by mass percentage.

Fluazinam 20%;

Thiaprodil 20%;

Calcium dodecylbenzenesulfonate 3%

Sodium Dibutyl Naphthalene Sulfonate 4%;

Silica 10%;

Kaolin balance;

Preparation method: Put the above components into the mixer according to the proportion of the formula and mix them evenly, then carry out jet milling. 60-80g per mu is used to control rice sheath blight and the effect can reach 86%.

Example 5

Preparation method of 30% fluazinam·thiazilamide wettable powder: by mass percentage.

Fluazinam 15%;

Thianodil 15%;

Alkyl naphthalene sulfonate formaldehyde condensate 4%

Sodium lignosulfonate 4%;

Silica 10%;

Attapulgite balance

Preparation method, with embodiment 4. 60-80g per mu is used to control rice sheath blight and the effect is about 84%.

Example 6

The preparation method of 10% fluazinam·thiazilamide water-dispersible granules: by mass percentage.

Fluazinam 5%;

Thianodil 5%;

Sodium oleic acid methyl taurine 5%;

Calcium lignosulfonate 2%;

Polyvinyl alcohol 4%

Ammonium sulfate 8%

Silica 10%;

Kaolin balance;

Preparation method: add the above components into a mixer according to the formula ratio and mix them evenly, then carry out jet crushing, add a small amount of purified water after the crushing is complete, stir well, extrude and granulate, and dry to get wettable powder products. 60-80g per mu is used to control rice sheath blight and the effect can reach 82%.

Example 7

The preparation method of 55% fluazinam·thiazilamide water-dispersible granules: by mass percentage.

Fluazinam 5%;

Thianodil 50%;

Sodium oleic acid methyl taurine 5%;

Calcium lignosulfonate 2%;

Polyvinyl alcohol 4%

Ammonium sulfate 3%

Kaolin balance;

Preparation method: add the above components into a mixer according to the formula ratio, mix them uniformly, and then carry out airflow crushing. After the crushing is completed, add a small amount of purified water to fully stir, extrude and granulate, and dry to obtain the water-dispersible granule product. 60-80g per mu is used to control rice sheath blight and the effect can reach 75%.

Example 8

The preparation method of 48% fluazinam·thiazilamide wettable powder: by mass percentage.

Fluazinam 45%;

Thianodil 3%;

Calcium dodecylbenzenesulfonate 5%

Sodium Dibutyl Naphthalene Sulfonate 3%;

Silica 6%;

Kaolin balance;

Preparation method: Put the above components into the mixer according to the proportion of the formula and mix them evenly, then carry out jet milling. 100-120g per mu is used to control rice sheath blight and the effect can reach 73%.

Example 9

The preparation method of 22% fluazinam·thiazilamide suspension concentrate: by mass percentage.

Fluazinam 2%;

Thiaprodil 20%;

Sodium Dibutyl Naphthalene Sulfonate 2%;

Castor oil ethoxylate 3%;

Polyvinyl alcohol 0.5%;

Phosphate ester defoamer 0.5%;

water balance.

Preparation method: according to the formula, weigh the above components in proportion, put into a ball mill and mill for 30 minutes, filter, pump into the dispersion tank for 3 minutes at high speed and then fully grind with a sand mill to control the particle diameter of the solid component within 2 microns , and stir evenly after grinding to obtain a suspension product. 60-80g per mu is used to control rice sheath blight and the effect can reach 75%.

Example 10

Preparation method of 30% fluazinam·thiazilamide wettable powder: by mass percentage.

Fluazinam 25%;

Thianodil 5%;

Alkyl naphthalene sulfonate formaldehyde condensate 4%

Calcium lignosulfonate 4%;

Silica 10%;

Attapulgite balance

Preparation method: Put the above components into the mixer according to the proportion of the formula and mix them evenly, then carry out jet milling. 60-80g per mu is used to control rice sheath blight and the effect is about 76%.

Example 11

The preparation method of 32% fluazinam·thiazilamide suspension concentrate: by mass percentage.

Fluazinam 2%;

Thianodil 30%;

Sodium lignosulfonate 5%;

Alkylphenol polyoxyethylene phosphate 4%;

Xanthan gum 0.5%;

Phosphate ester defoamer 0.4%;

water balance.

Preparation method: According to the formula, weigh the above components in proportion, add to a ball mill and mill for 30 minutes, filter, pump into the dispersion tank for 3 minutes at high speed, and then fully grind with a sand mill to control the particle diameter of the solid component within 2 microns , and stir evenly after grinding to obtain a suspension product. 60-80g per mu is used to control rice sheath blight and the effect can reach 74%.

Example 12

Preparation method of 24% fluazinam·thiazilamide wettable powder: by mass percentage.

Fluazinam 4%;

Thiaprodil 20%;

Calcium dodecylbenzenesulfonate 2%

Sodium Dibutyl Naphthalene Sulfonate 2%;

Silica 4%;

Kaolin balance;

Preparation method: Put the above components into the mixer according to the proportion of the formula and mix them evenly, then carry out jet milling. 100-120g per mu is used to control rice sheath blight and the effect can reach 78%.

Claims (5)

1. A bactericidal composition containing fluazinam and tiiazil, characterized in that it contains the active components fluazinam and tiiazil in a mass ratio of 5:1 to 1:5. 2. The bactericidal composition according to claim 1, characterized in that: it is added with commonly used agricultural bactericide auxiliaries to make a commonly used agricultural bactericidal composition dosage form. 3. The bactericidal composition according to claim 2, characterized in that: the dosage form of the bactericidal composition is water suspension concentrate, wettable powder or water dispersible granule. 4. The bactericidal composition according to claim 2, characterized in that: the active ingredients composed of fluazinam and tiiazil account for 10%-60% of the total mass of the bactericidal composition. 5. The fungicidal composition according to any one of claims 1-4 is used to control crop fungal diseases of rice sheath blight.