CN104430361A - Fungicidal composition containing JS399-19 and fluazinam - Google Patents

Abstract

The invention discloses a novel fungicide composition containing cyclostrobin and fluazinam. The mass ratio of the active components cyclostrobin and fluazinam is 15:1-1:15. The present invention has better control effects on crop fungal diseases such as wheat head blight, and the combined use of the two agents can reduce the dosage, effectively reduce ecological damage and environmental pollution, and can improve crop yield and quality; Strostrobin and fluazinam are both of low toxicity, safe for humans, animals, and beneficial organisms; they can delay the resistance of bacteria to single agents. Cyclostrobin and fluazinam have different structural types and different mechanisms of action. Combining the two in a certain proportion has high bactericidal activity in actual use, and can avoid cyclostrobin and fluazinam to a greater extent. When fluazinam is used alone, the selection pressure on pathogens can delay the emergence and development of drug resistance of pathogens, and reduce the total dosage per unit area, which is economical, efficient and environmentally friendly.

Description

A bactericidal composition containing cyclostrobin and fluazinam

technical field

The invention belongs to the field of agricultural fungicides, and in particular relates to a fungicidal composition of cyclostrobin and fluazinam.

Background technique

Cyclostrobin, molecular formula C12H12N2O2, chemical name: ethyl 2-cyano-3-aminoacrylate, has a novel mechanism of action, and is compatible with benzimidazoles, dithiocarbamates, ergosterol biosynthesis inhibitors, Various fungicides, such as methoxyacrylate, have different mechanisms of action, and have the functions of protection, treatment, systemic absorption, transport, etc., and can effectively control plant diseases caused by the above-mentioned types of fungicide-resistant strains. It has high biological activity against Fusarium graminearum, Fusarium moniliforme, and Fusarium oxysporum of the Fusarium genus, and is suitable for wheat scab, sheath blight, pepper phytophthora, rice bakanae disease, and cucumber ash Effective control of mildew, watermelon wilt and other crop diseases, cucumber downy mildew caused by Cuban pseudo-downy mildew, rice sheath blight caused by Xanthomonas oryzae, pepper anthracnose caused by capsicum anthracnose, Berenger grape Sclerotinia sclerotiorum caused by the sclerotium also has a certain inhibitory effect.

Fluazinam (English common name: fluazinam), the molecular formula is C ₁₃ H ₄ C _l2 F ₆ N ₄ O ₄ ,

The common chemical name is 3-chloro-N-(3-chloro-5-trifluoromethyl-2-pyridyl)-α,α,α-trifluoro-2,6-dinitro-p-toluidine, It is a 2,6-dinitroaniline compound, which is a protective fungicide and can prevent and control diseases caused by Botrytis cinerea. It is very effective against Alternaria, Botrytis, Phytophthora, Plasmodium, Sclerotinia and Nigella sp., against benzimidazoles and dicarboximide fungicides of gray grapes Spores also have good effects, are resistant to rain erosion, have a long duration, and have excellent control effects on plant-eating mites. They also have excellent control effects on clubroot of cruciferous plants. It also has a good preventive effect on diseases.

Contents of the invention

The purpose of the present invention is to propose a novel fungicide composition containing cyprostrobin and fluazinam, which is used for preventing and treating various crop diseases such as wheat head blight. Cyclostrobin and fluazinam have different structural types and different mechanisms of action. Combining the two in a certain proportion has high bactericidal activity in actual use, and can avoid cyclostrobin and fluazinam to a greater extent. When fluazinam is used alone, the selection pressure on pathogens can delay the emergence and development of drug resistance of pathogens, and reduce the total dosage per unit area, which is economical, efficient and environmentally friendly.

The bactericidal composition containing cyclostrobin and fluazinam of the present invention comprises active components cyclostrobin and fluazinam in a mass ratio of 15:1 to 1:15.

Preferably, the mass ratio of cyprostrobin to fluazinam is 10:1-1:10, more preferably 5:1-1:5, most preferably 1:1-1:5.

The composition of the present invention is added with commonly used agricultural fungicide auxiliaries to prepare a commonly used agricultural fungicide composition dosage form.

The dosage form of the bactericidal composition can be water suspension, wettable powder, water emulsion or water dispersible granule. The agricultural fungicide additives used include various additives such as wetting agents, dispersants, thickeners, defoamers, fillers and solvents. The wetting agents and dispersants used can be calcium dodecylbenzenesulfonate , Sodium Butylene Naphthalene Sulfonate, Sodium Lignosulfonate, Calcium Lignosulfonate, Alkylphenol Polyoxyethylene Ether, Alkylphenol Polyoxyethylene Phosphate, Castor Oil Polyoxyethylene Ether, etc. One or more kinds; the thickener used can be xanthan gum, polyvinyl alcohol, etc.; the defoamer used can be one or more of phosphate defoamers and silicone defoamers; the used The filler can be one or more of white carbon black, kaolin, attapulgite, etc.; the solvent used is N,N dimethylformamide, methyl oleate, propylene glycol, etc.

The active component composed of cyclostrobin and fluazinam accounts for 10%-60% of the total mass of the bactericidal composition, preferably 10%-30%.

The fungicidal composition of the invention can be used for preventing and treating fungal diseases of crops such as wheat head blight.

Through the careful analysis of the action mechanism and bactericidal properties of cyclostrobin and fluazinam, the Wadley method was used to design the experiment and process the results. First, measure the EC ₅₀ of two single compounds (hereinafter referred to as drugs) of cyprostrobin and fluazinam, and set the combination ratio of the two drugs according to the Wadley method according to the EC ₅₀ of the two single drugs, and determine the SR value according to the Wadley synergy index. The appropriate composition ratio is then verified by Sun Yunpei's method.

The target object of the test is Fusarium rubella. The test uses the colony diameter method. Take the mother solution of cyclostrobin and fluazinam, and configure it into a plate of the required drug solution, about 14ml per plate, and set a plate without drug control (CK), and repeat 3 plates for each treatment, that is, repeat 3 times. At the edge of the pre-cultured colony, use a sterilized hole puncher to prepare mycelium pieces with a diameter of 5mm, move them to different medium plates, culture at 28°C for 4 days, measure the diameter of the colony (mm), and calculate the growth inhibition rate by the following formula :

Convert the mycelial growth inhibition rate into the inhibition probability value (Y), convert the drug concentration into the concentration logarithm (X), and draw the virulence regression line according to the concentration logarithm as the abscissa and the probability value as the ordinate, to obtain the Toxicity regression equation of ester and fluazinam single agent and its mixture against wheat head blight, and calculate EC ₅₀ , EC ₉₀ value and correlation coefficient r value.

According to the Wadley method to evaluate the interaction of the mixture, the calculation formula is as follows:

EC _{50 (theoretical value)} = (a+b)/(a/EC _50a + b/EC _50b ), SR = EC _{50 (theoretical value)} /EC _{50 (actual value)} .

Where a and b are the content ratios of each component in the mixture, and the effect of the mixture is analyzed by SR value. If SR≤0.5, the mixture of the two agents has an antagonistic effect; if SR=0.5-1.5, the mixture of the two agents has an additive effect; if SR≥1.5, the mixture of the two agents has a synergistic effect.

Table 1 The results of determination of the potency of cyprostrobin and fluazinam single agent and their mixture against wheat gibberellic virus

From the data in Table 1, it can be concluded that when cyprostrobin and fluazinam are mixed at a ratio of 15:1 to 1:15, the growth of wheat scab mycelium can be inhibited, and when mixed at a ratio of 1:5, it can be compared Strongly inhibit the growth of wheat scab mycelium, showing synergistic effect.

The present invention has better control effects on crop fungal diseases such as wheat head blight, and the combined use of the two agents can reduce the dosage, effectively reduce ecological damage and environmental pollution, and can improve crop yield and quality; Strostrobin and fluazinam are both of low toxicity, safe for humans, animals, and beneficial organisms; they can delay the resistance of bacteria to single agents.

Detailed ways

The following examples are a further description of the content of the present invention as an explanation of the technical content of the present invention, but the essential content of the present invention is not limited to the following examples, those of ordinary skill in the art can and should know any Simple changes or replacements of the essential spirit of the invention shall fall within the scope of protection required by the present invention.

Example 1

The preparation method of 30% cyclostrobin·fluazinam aqueous suspension concentrate: by mass percentage.

Cyclostrobin 5%;

Fluazinam 25%;

Sodium lignosulfonate 6%;

Alkylphenol polyoxyethylene phosphate 3%;

Xanthan Gum 0.2%;

Silicone defoamer 0.4%;

Water surplus.

Preparation method: According to the formula, weigh the above components in proportion, add to a ball mill and mill for 30 minutes, filter, pump into the dispersion tank for 3 minutes at high speed, and then fully grind with a sand mill to control the particle diameter of the solid component within 2 microns , and stir evenly after grinding to obtain a suspension product. 60-80g per mu is used to prevent and control wheat scab, and the effect can reach more than 88%.

Example 2

The preparation method of 24% cyclostrobin·fluazinam aqueous suspension concentrate: by mass percentage.

Cyclostrobin 12%;

Fluazinam 12%;

Sodium lignosulfonate 3%;

Castor oil polyoxyethylene ether 3%;

Polyvinyl alcohol 0.2%;

Phosphate ester defoamer 0.3%;

Water balance.

Preparation method, with embodiment 1. 60-80g per mu is used to control wheat scab and the effect can reach 76%.

Example 3

The preparation method of 30% cyclostrobin·fluazinam aqueous suspension concentrate: by mass percentage.

Cyclostrobin 25%;

Fluazinam 5%;

Sodium Butylene Naphthalene Sulfonate 4%;

Castor oil polyoxyethylene ether 3%;

Polyvinyl alcohol 0.2%;

Phosphate ester defoamer 0.4%;

Water balance.

Preparation method, with embodiment 1. 60-80g per mu is used to prevent and control wheat scab, and the effect can reach 80%.

Example 4

The preparation method of 40% cyclostrobin·fluazinam wettable powder: by mass percentage.

Cyclostrobin 20%;

Fluazinam 20%;

Calcium Lignosulfonate 2%

Sodium Butylene Naphthalene Sulfonate 3%;

Silica 10%;

Kaolin balance;

Preparation method: Add the above components into the mixer according to the formula ratio, mix them evenly, and then carry out jet milling. After the milling is completed, stir well and evenly to get wettable powder products. 60-80g per mu is used to control wheat scab and the effect can reach 86%.

Example 5

The preparation method of 12% cyclostrobin·fluazinam aqueous emulsion: by mass percentage.

Cyclostrobin 2%;

Fluazinam 10%;

Calcium dodecylbenzenesulfonate 4%

Castor oil polyoxyethylene ether 6%;

N,N dimethylformamide 20%;

Methyl Oleate 20%

Propylene Glycol 5%

Water balance

The preparation method is to dissolve cyclostrobin, fluazinam, and N,N dimethylformamide, then mix with methyl oleate, calcium dodecylbenzenesulfonate, and castor oil polyoxyethylene ether, and introduce The aqueous phase was sheared at high speed for 30 minutes, and filtered to obtain the finished product. 60-80g per mu is used to control wheat head blight and the effect is about 80%.

Example 6

The preparation method of 10% cyclostrobin·fluazinam water-dispersible granule: by mass percentage.

Cyclostrobin 5%;

Fluazinam 5%;

Calcium lignosulfonate 6%;

Polyvinyl alcohol 4%

Ammonium sulfate 8%

Silica 10%;

Kaolin balance;

Preparation method: add the above components into a mixer according to the formula ratio, mix them evenly, and then carry out jet milling. After the milling is completed, add a small amount of purified water to fully stir evenly, extrude and granulate, and dry to obtain the water-dispersible granule product. 60-80g per mu is used to control wheat scab and the effect can reach 82%.

Claims (7)

1. a bactericidal composition for 2-cyano-3-amino-3-phenylancryic acetate-containing and fluazinam, is characterized in that: include active component 2-cyano-3-amino-3-phenylancryic acetate and fluazinam that mass ratio is 15:1 ~ 1:15.

2. bactericidal composition according to claim 1, is characterized in that: the mass ratio of described 2-cyano-3-amino-3-phenylancryic acetate and fluazinam is 10:1 ~ 1:10.

3. bactericidal composition according to claim 2, is characterized in that: the mass ratio of described 2-cyano-3-amino-3-phenylancryic acetate and fluazinam is 5:1 ~ 1:5.

4. the bactericidal composition according to any one of claim 1-3, is characterized in that: be added with conventional agricultural bactericidal agent aid and make agriculturally conventional bactericidal composition formulation.

5. bactericidal composition according to claim 4, is characterized in that: described bactericidal composition formulation is aqueous suspension agent, wetting powder, aqueous emulsion or water-dispersible granules.

6. bactericidal composition according to claim 4, is characterized in that: the active component of described 2-cyano-3-amino-3-phenylancryic acetate and fluazinam composition accounts for 10% ~ 60% of bactericidal composition gross mass.

7. the bactericidal composition described in any one of claim 1-6 is for preventing and treating the crop fungal disease of wheat scab.