CN104961244A - Treatment method for L-arginine fermentation waste liquid - Google Patents
Treatment method for L-arginine fermentation waste liquid Download PDFInfo
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- CN104961244A CN104961244A CN201510380652.7A CN201510380652A CN104961244A CN 104961244 A CN104961244 A CN 104961244A CN 201510380652 A CN201510380652 A CN 201510380652A CN 104961244 A CN104961244 A CN 104961244A
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- 238000000034 method Methods 0.000 title claims abstract description 34
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- 229930064664 L-arginine Natural products 0.000 title claims abstract description 19
- 235000014852 L-arginine Nutrition 0.000 title claims abstract description 18
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- 229910021536 Zeolite Inorganic materials 0.000 claims description 12
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 12
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- 239000005995 Aluminium silicate Substances 0.000 claims description 8
- 235000012211 aluminium silicate Nutrition 0.000 claims description 8
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- 239000002994 raw material Substances 0.000 claims description 5
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 4
- 239000012467 final product Substances 0.000 claims description 4
- 238000013467 fragmentation Methods 0.000 claims description 4
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- 230000001105 regulatory effect Effects 0.000 claims description 4
- 241000588626 Acinetobacter baumannii Species 0.000 claims description 2
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- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 2
- 241001135759 Sphingomonas sp. Species 0.000 claims description 2
- 235000001014 amino acid Nutrition 0.000 abstract description 11
- 150000001413 amino acids Chemical class 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 5
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- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The invention belongs to the technical field of amino acid fermentation wastewater treatment and discloses a treatment method for L-arginine fermentation waste liquid. The treatment method comprises the following steps: step 1) preparing a microbial carrier; step 2) preparing a microbial preparation; and step 3) carrying out microbial treatment. The method for treating waste liquid disclosed by the invention is simple and feasible, good in effect, low in cost and easy to be accepted by enterprises.
Description
Technical field
The invention belongs to Amino Acid Fermentation Wastewater processing technology field, be specifically related to a kind for the treatment of process of L-arginine fermented waste fluid.
Background technology
The chemical formal name used at school of arginine (arginine, Arg): (S)-2-Amino-5-guanidinopentanoic acid is a kind of polarity alpha amino acid containing guanidine radicals of aliphatic alkalescence, positively charged in physiological conditions; L-arginine is the coded amino acid in protein synthesis.For Mammals, arginine is classified as half necessary or amino acid that conditionality is necessary, and depend on biological etap and healthy state and determine, natural product are present in protamine in a large number, are also the essentially consist of various protein.
L-arginine is described as " magical molecule " by scientist, and it is one of basic aminoacids, for the adult of health, is nonessential amino acid, but arginine maintains the requisite amino acid of infant growth; Be the intermediate metabolites of urea cycle, ammonia can be impelled to be transformed into urea, thus reduce Blood Ammonia Concentration, when body development is not yet ripe or under health stressed condition, as lacked arginine, body just can not complete physiological function; Patient lacks arginine can cause that blood ammonia is too high even goes into a coma, and baby lacks arginine then can not normal development and growth.After Nobel Prize in medicine in 1998 has admitted the medical efficacy of L-arginine, L-arginine has caused the interest of medical drug field madness, and Columbia Univ USA was once likened to " the magical bullet " of cardiovascular systems; The discovery of L-arginine makes grow up shortage, hypertension, sexuality of heart trouble, immunologic function, obesity, gene go down and the treatment of the symptom such as the mankind are aging obtains very large improvement.
Along with the fast development of amino acid industry in recent years, surge to amino acid whose demand, China has become the maximum amino acids production state in the whole world.In amino acids production, because flushing membrane is considered containing higher ammonia nitrogen in the high-concentration waste water of equipment, how denitrogenation is its key problem, does not have good way at present to the process of this waste water.Chinese patent CN104222704A and CN104261947A has prepared feed and fertilizer etc. with fermentation waste water respectively, and aforesaid method design is unique, is applicable to the enterprise possessing fertilizer or fodder production qualification and ability.
Traditional denitrification process is usually by oxygen-starved area with aerobicly to distinguish, as Prepositive denitrification, rear-mounted denitrification, A/O method, A
2o method, SBR etc., but due to nitrobacteria growth speed comparatively slow, limit the raising of nitric efficiency, the shortcomings such as when bacteria adhension poor reliability and denitrification carbon source is not enough fail to promote.Meanwhile, the process also more difficult control of nitrification and denitrification, and also traditional technique also also exists, and initial cost is large, working cost is high, a series of drawback such as energy wastage and emission greenhouse gas.But the progress of the discovery of aerobic denitrifying bacteria, nitrification bacteria and aerobic denitrification, heterotrophic nitrification and autotrophic denitrification is Simultaneous Nitrification and denitrification has established theoretical basis.Directed structure can carry out the high effective microbial community of biochemistry/nitrated/denitrification in same reaction unit simultaneously, adapt to fermentation high density factory effluent comprising screening and enrichment, have the special efficacy aerobic denitrifying bacteria of degraded advantage, reach the microcirculation forming material between whole microbial strains and mutually utilize, allow COD as must being utilized of carbon source in biochemistry/nitrated/anti-nitration reaction, improve the biological efficiency of sewage disposal comprehensively.The technology of therefore carrying out simultaneous nitrification-denitrification (SND) under aerobic condition becomes current biological denitrificaion and opens new technology.
Biotechnology process fermentation waste water, it possesses with low cost, can not cause the advantages such as secondary pollution, but it is unreasonable to there is microorganism compatibility, processing efficiency is low, and microbe carrier exists internal mass transfer difference, not easily printing opacity, the defects such as specific surface area is little, and work-ing life is short; Biotechnological formulation needs to throw in often, adds cost, and injected volume is large, and the sludge quantity of generation is large, is not easy process.
Summary of the invention
The object of the invention is the deficiency for traditional technology, provide a kind for the treatment of process of L-arginine fermented waste fluid, the method treatment effect is good, and long service life is with low cost, alleviates business burden, brings huge economic benefit and environmental benefit.In order to realize the object of the invention, adopt following technical scheme:
In order to realize the object of the invention, the present invention is achieved by the following technical solutions:
A treatment process for L-arginine fermented waste fluid, it comprises the steps:
Step 1) prepares microbe carrier, step 2) prepare microbial preparation, step 3) microbiological treatment.
Particularly, comprise the steps:
Step 1) prepares microbe carrier:
(1) following each raw material for standby is taken according to weight part, wherein, water 20-30 part, brown coal 10-12 part, kaolin 8-10 part, zeolite powder 3-5 part, chitin 2-3 part, silica-gel powder 1-2 part; Preferably, the particle diameter of zeolite powder and silica-gel powder is 200 orders, and the particle diameter of chitin is 50 orders;
(2) brown coal crusher is carried out fragmentation, then add kaolin, mix, be ground to 100 object powder, be material 1;
(3) in zeolite powder, add the 1M calcium chloride solution of identical weight, stir, then 350 DEG C of roastings 3 hours, obtain material 2;
(4) by water, material 1, material 2, chitin and silica-gel powder add in stirred reactor successively, and 500 turns/min stirs 10min, leave standstill 30min, then 200 turns/min stirs 3min, and being finally placed in 60 DEG C of oven dry to moisture contents is 5%(parts by weight), obtain microbe carrier;
Step 2) prepare microbial preparation:
By subtilis, it is 1 × 10 that Acinetobacter bauamnnii and Sphingomonas are cultured to concentration respectively
7the bacterium liquid of individual/ml, scenedesmus obliquus being cultured to concentration is 1 × 10
5the algae liquid of individual/ml, mixes the subtilis liquid of above-mentioned cultivation, Acinetobacter bauamnnii liquid, Sphingomonas liquid and the scenedesmus obliquus liquid volume ratio according to 5:3:2:1, leaves standstill 6 hours, obtains mixing liquid; Microbe carrier mixing liquid and step 1) prepared is according to the quality of 1:2 than mixing and stirring, and room temperature fermentation 24-36h, being dried to water content is 10%(parts by weight), to obtain final product.
Step 3) microbiological treatment:
The pH regulating L-arginine fermented waste fluid is 7-7.5, then according to 5g microbial preparation: the addition of 1 cubic metre of waste liquid adds microbial preparation, then leaves standstill six days, discharges.
Bacterial classification of the present invention and algae all commercial sources can be bought and obtain from preservation center etc.
Bacterial classification of the present invention and algae all obtain bacterium liquid or the algae liquid of desired concn by the cultural method of routine, this is innovative point of the present invention not, as space is limited, does not repeat one by one.
The beneficial effect that the present invention obtains mainly comprises:
The method simple possible of process fermentation waste water of the present invention, with low cost, treatment effect is good;
Carrier provided by the invention, adopts the raw material of different-grain diameter, can not only expand the specific surface area of carrier, and have tensile strength large, be evenly distributed, specific surface area is large, the features such as long service life;
Carrier provided by the invention can improve the adhesion amount of microorganism greatly, and increase the biofilm biomass of overall attachment, the microorganism concn in reactive tank is improved, and can reduce mud generation;
The existence of the anaerobism that carrier provided by the invention provides, anoxic and aerobic various environment can promote nitration denitrification effect, promotes the minimizing of mud simultaneously, is conducive to the removal of the pollutent such as ammonia nitrogen in waste liquid;
Biotechnological formulation of the present invention, by various bacterial classification and the algae that can form dominant microflora, is mixed with high-performance bio preparation, reasonable compatibility between each microorganism, and symbiosis is coordinated, mutually not antagonism, and biomass is large, and breeding is fast.
Biotechnological formulation of the present invention is suitable for Wastewater treating, and be convenient to transport and preserve, more easily activate during use, running cost is cheap.
Embodiment
Technical scheme in the application is understood better in order to make those skilled in the art person, below in conjunction with the application's specific embodiment, the technical scheme of the application is clearly and completely described, obviously, described embodiment is only some embodiments of the present application, instead of whole embodiments.Based on the embodiment in the application, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, should belong to the scope of protection of the invention.
Embodiment 1
A treatment process for L-arginine fermented waste fluid, it comprises the steps:
Step 1) prepares microbe carrier, step 2) prepare microbial preparation, step 3) microbiological treatment.
Particularly, comprise the steps:
Step 1) prepares microbe carrier:
(1) following each raw material for standby is taken according to weight part, wherein, 20 parts, water, 10 parts, brown coal, kaolin 8 parts, zeolite powder 3 parts, chitin 2 parts, silica-gel powder 1 part; Wherein, the particle diameter of zeolite powder and silica-gel powder is 200 orders, and the particle diameter of chitin is 50 orders;
(2) brown coal crusher is carried out fragmentation, then add kaolin, mix, be ground to 100 object powder, be material 1;
(3) in zeolite powder, add the 1M calcium chloride solution of identical weight, stir, then 350 DEG C of roastings 3 hours, obtain material 2;
(4) by water, material 1, material 2, chitin and silica-gel powder add in stirred reactor successively, and 500 turns/min stirs 10min, leave standstill 30min, then 200 turns/min stirs 3min, and being finally placed in 60 DEG C of oven dry to moisture contents is 5%(parts by weight), obtain microbe carrier;
Step 2) prepare microbial preparation:
By subtilis, it is 1 × 10 that Acinetobacter bauamnnii and Sphingomonas are cultured to concentration respectively
7the bacterium liquid of individual/ml, scenedesmus obliquus being cultured to concentration is 1 × 10
5the algae liquid of individual/ml, mixes the subtilis liquid of above-mentioned cultivation, Acinetobacter bauamnnii liquid, Sphingomonas liquid and the scenedesmus obliquus liquid volume ratio according to 5:3:2:1, leaves standstill 6 hours, obtains mixing liquid; Microbe carrier mixing liquid and step 1) prepared is according to the quality of 1:2 than mixing and stirring, and room temperature fermentation 36h, being dried to water content is 10%(parts by weight), to obtain final product.
Step 3) microbiological treatment:
The pH regulating L-arginine fermented waste fluid is 7.5, then according to 5g microbial preparation: the addition of 1 cubic metre of waste liquid adds microbial preparation, then leaves standstill six days, discharges.
Embodiment 2
A treatment process for L-arginine fermented waste fluid, it comprises the steps:
Step 1) prepares microbe carrier, step 2) prepare microbial preparation, step 3) microbiological treatment.
Particularly, comprise the steps:
Step 1) prepares microbe carrier:
(1) following each raw material for standby is taken according to weight part, wherein, 30 parts, water, 12 parts, brown coal, kaolin 10 parts, zeolite powder 5 parts, chitin 3 parts, silica-gel powder 2 parts; Wherein, the particle diameter of zeolite powder and silica-gel powder is 200 orders, and the particle diameter of chitin is 50 orders;
(2) brown coal crusher is carried out fragmentation, then add kaolin, mix, be ground to 100 object powder, be material 1;
(3) in zeolite powder, add the 1M calcium chloride solution of identical weight, stir, then 350 DEG C of roastings 3 hours, obtain material 2;
(4) by water, material 1, material 2, chitin and silica-gel powder add in stirred reactor successively, and 500 turns/min stirs 10min, leave standstill 30min, then 200 turns/min stirs 3min, and being finally placed in 60 DEG C of oven dry to moisture contents is 5%(parts by weight), obtain microbe carrier;
Step 2) prepare microbial preparation:
By subtilis, it is 1 × 10 that Acinetobacter bauamnnii and Sphingomonas are cultured to concentration respectively
7the bacterium liquid of individual/ml, scenedesmus obliquus being cultured to concentration is 1 × 10
5the algae liquid of individual/ml, by the subtilis liquid of above-mentioned cultivation, Acinetobacter bauamnnii liquid, Sphingomonas liquid and scenedesmus obliquus liquid mix according to the volume ratio of 5:3:2:1, leave standstill 6 hours, obtain mixing liquid; Microbe carrier mixing liquid and step 1) prepared is according to the quality of 1:2 than mixing and stirring, and room temperature fermentation 24h, being dried to water content is 10%(parts by weight), to obtain final product.
Step 3) microbiological treatment:
The pH regulating L-arginine fermented waste fluid is 7, then according to 5g microbial preparation: the addition of 1 cubic metre of waste liquid adds microbial preparation, then leaves standstill six days, discharges.
Wherein, subtilis is (Bacillus subtilis) CGMCC NO:2947 (can see CN101838621A), and Acinetobacter bauamnnii is (Acinetobacter baumanii) for ATCC 19606(can see Infect Immun. 2012 Mar; 80 (3): 1015-24), Sphingomonas is that (Sphingomonas sp.) CGMCC NO.4589(can see CN102168054A), scenedesmus obliquus is that (Scenedesmus obliquus) CGMCC No.8015(can see CN103484374A).
Embodiment 3
Effect test of the present invention:
With the fermented waste fluid removing the materials such as fermentation thalli, albumen and polysaccharide for processing sample is tested.
Control group 1: use diatomite support; Microorganism type is identical with test group; Processing mode: after adding three days continuously, every day 5g/ cubic meter, then leave standstill six days, check processing the results are shown in Table 1.
Control group 2: carrier is identical with embodiment 2, difference is, only adds three kinds of bacterium, does not add scenedesmus obliquus; Processing mode: first day adds 5g/ cubic meter, then leave standstill six days, check processing the results are shown in Table 1.
Test group: microbial preparation prepared by the embodiment of the present invention 2; Processing mode: after adding one day, 5g/ cubic meter, then leave standstill six days, check processing the results are shown in Table 1.
Table 1
| Before process (mg/L) | After process (control group 1) (mg/L) | After process (control group 2) (mg/L) | (test group) (mg/L) after process | |
| COD | 1517 | 17.9 | 52.2 | 5.9 |
| NH3-N | 223 | 15.4 | 17.3 | 2.1 |
| Sulfide | 27.4 | 5.9 | 3.1 | 1.3 |
| P 2O 5 | 12.8 | 2.3 | 3.7 | 0.6 |
| Chlorion | 26.9 | 4.3 | 5.6 | 0.4 |
Conclusion: microbe carrier surface irregularity prepared by the present invention, specific surface area is large, and microorganic adhesion is effective, and drops into number of times and weight all reduces, and long service life, without the need to repeatedly throwing in, having saved cost, having reduced the generation of mud.Mushroom and algae reasonable compatibility in microbial preparation of the present invention, effectively can remove each pollutant in fermented waste fluid, reach emission standard completely.The method simple possible that the present invention disposes waste liquid, effective, with low cost, easily accept by enterprise.
Although above done detailed explanation with general explanation and embodiment to this case, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, amendment done without departing from theon the basis of the spirit of the present invention or improvement, all belong to the scope of protection of present invention.
Claims (4)
1. a treatment process for L-arginine fermented waste fluid, is characterized in that, described method comprises the steps:
Step 1) prepares microbe carrier, step 2) prepare microbial preparation, step 3) microbiological treatment.
2. method according to claim 1, is characterized in that, described method comprises the steps:
Step 1) prepares microbe carrier:
(1) following each raw material for standby is taken according to weight part, wherein, water 20-30 part, brown coal 10-12 part, kaolin 8-10 part, zeolite powder 3-5 part, chitin 2-3 part, silica-gel powder 1-2 part;
(2) brown coal crusher is carried out fragmentation, then add kaolin, mix, be ground to 100 object powder, be material 1;
(3) in zeolite powder, add the 1M calcium chloride solution of identical weight, stir, then 350 DEG C of roastings 3 hours, obtain material 2;
(4) by water, material 1, material 2, chitin and silica-gel powder add in stirred reactor successively, and 500 turns/min stirs 10min, leave standstill 30min, then 200 turns/min stirs 3min, and being finally placed in 60 DEG C of oven dry is 5% to moisture content, obtains microbe carrier;
Step 2) prepare microbial preparation:
By subtilis, it is 1 × 10 that Acinetobacter bauamnnii and Sphingomonas are cultured to concentration respectively
7the bacterium liquid of individual/ml, scenedesmus obliquus being cultured to concentration is 1 × 10
5the algae liquid of individual/ml, mixes the subtilis liquid of above-mentioned cultivation, Acinetobacter bauamnnii liquid, Sphingomonas liquid and the scenedesmus obliquus liquid volume ratio according to 5:3:2:1, leaves standstill 6 hours, obtains mixing liquid; Microbe carrier prepared by mixing liquid and step 1) according to the quality of 1:2 than mixing and stirring, room temperature fermentation 24-36h, being dried to water content is 10%, to obtain final product;
Step 3) microbiological treatment:
The pH regulating L-arginine fermented waste fluid is 7-7.5, then according to 5g microbial preparation: the addition of 1 cubic metre of waste liquid adds microbial preparation, then leaves standstill six days, discharges.
3. method according to claim 2, is characterized in that, preferably, the particle diameter of described zeolite powder and silica-gel powder is 200 orders, and the particle diameter of described chitin is 50 orders.
4. according to the method in claim 2 or 3, it is characterized in that, described subtilis (Bacillus subtilis) is CGMCC NO:2947, described Acinetobacter bauamnnii (Acinetobacter baumanii) is ATCC 19606, described Sphingomonas (Sphingomonas sp.) is CGMCC NO.4589, and described scenedesmus obliquus (Scenedesmus obliquus) is CGMCC No.8015.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510380652.7A CN104961244B (en) | 2015-07-02 | 2015-07-02 | A kind of processing method of L arginine fermented waste fluid |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1305787C (en) * | 2002-09-27 | 2007-03-21 | 上海金迪生物技术工程有限公司 | A method for comprehensive treatment of wastewater |
| CN101428930A (en) * | 2008-12-09 | 2009-05-13 | 中南大学 | Treatment process for hydrargyrum-containing flue gas washing wastewater from nonferrous metal smelting with biological agent |
| JP4516025B2 (en) * | 2003-11-21 | 2010-08-04 | 荏原エンジニアリングサービス株式会社 | Method and apparatus for producing / recovering magnesium ammonium phosphate |
| CN102703420A (en) * | 2010-11-29 | 2012-10-03 | 王祥红 | Microbial degradation preparation for municipal garbage and sewage and preparation method thereof |
| CN104230004A (en) * | 2014-10-16 | 2014-12-24 | 内蒙古阜丰生物科技有限公司 | Biological agent for processing glutamic acid fermentation waste water |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1305787C (en) * | 2002-09-27 | 2007-03-21 | 上海金迪生物技术工程有限公司 | A method for comprehensive treatment of wastewater |
| JP4516025B2 (en) * | 2003-11-21 | 2010-08-04 | 荏原エンジニアリングサービス株式会社 | Method and apparatus for producing / recovering magnesium ammonium phosphate |
| CN101428930A (en) * | 2008-12-09 | 2009-05-13 | 中南大学 | Treatment process for hydrargyrum-containing flue gas washing wastewater from nonferrous metal smelting with biological agent |
| CN102703420A (en) * | 2010-11-29 | 2012-10-03 | 王祥红 | Microbial degradation preparation for municipal garbage and sewage and preparation method thereof |
| CN104230004A (en) * | 2014-10-16 | 2014-12-24 | 内蒙古阜丰生物科技有限公司 | Biological agent for processing glutamic acid fermentation waste water |
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Denomination of invention: A treatment method of L-arginine fermentation waste liquid Effective date of registration: 20211018 Granted publication date: 20170301 Pledgee: China Construction Bank Corporation Junan sub branch Pledgor: SHANDONG FUFENG FERMENTATION Co.,Ltd. Registration number: Y2021370000120 |
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Date of cancellation: 20230414 Granted publication date: 20170301 Pledgee: China Construction Bank Corporation Junan sub branch Pledgor: SHANDONG FUFENG FERMENTATION CO.,LTD. Registration number: Y2021370000120 |