CN104950107B - A kind of test strips that can detect urea and melamine simultaneously - Google Patents
A kind of test strips that can detect urea and melamine simultaneously Download PDFInfo
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- CN104950107B CN104950107B CN201410115323.5A CN201410115323A CN104950107B CN 104950107 B CN104950107 B CN 104950107B CN 201410115323 A CN201410115323 A CN 201410115323A CN 104950107 B CN104950107 B CN 104950107B
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- melamine
- urea
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
The invention discloses the test strips that one kind can detect urea and melamine simultaneously, the test strips are divided into urea detection part and melamine detection part.The test strips can detect urea and melamine in milk powder or milk simultaneously, to improve the detection efficiency of harmful substance in milk powder or milk, reduce sample dosage, shorten detection time.The test strips can also filter the impurity particle not readily dissolved in milk powder, improve the accuracy of detection, easy to operate, be suitable for the large-scale promotion in average family.
Description
Technical field
The invention belongs to field of food detection, and in particular to one kind can detect the test strips of urea and melamine simultaneously.
Background technique
Currently, China's food-safety problem becomes increasingly conspicuous, wherein the adulterated problem of dairy products receives much attention, and milk powder is adulterated to be exactly
One common problem.One major criterion of quality of milk powder detection is protein content, and common can be improved detection
Harmful additive of data includes urea and melamine.
Studies have shown that taken long-term melamine may cause fecundity damage, bladder or kidney stone, bladder cancer.
In September, 2008, China's outburst Sanlu baby milk powder contamination accident, causes the infant for having eaten contaminated milk powder to generate kidney knot
Stone illness, main cause are exactly in milk powder containing the melamine severely exceeded.And the urea of human body excess intake will lead to
Liver, kidney, the organ injuries such as alveolar.
Dry milk component detection at present is still based on chemical analysis method, although its inspection result is accurate, process time-consuming is taken
Power destroys sample.Urea detection in, widely used is mid-infrared light spectral technology, with easy logistics progress,
The cost performances advantages such as the ornamental that batch samples processing capacity and analysis are possessed itself, but performance is analyzed less than it
Its conventional method of analysis, and need more complicated instrument.It is existing detection melamine method mainly with chromatography such as
GC-MS, HPLC, LC-MS etc., but these methods are unsuitable for scene and carry out large batch of quick detection, are less suitable for
Common family's self-test uses.Therefore, become research weight currently based on the test paper of the detection melamine of immunology principle research and development
Point.
But this kind of test paper are only capable of detection melamine this kind harmful substance, to being added simultaneously in milk powder or milk
Urea have no idea to detect.This results in such test paper low to the detection efficiency of harmful substance in milk powder or milk, sample
Dosage increases, so as to cause testing cost increase.Simultaneously as there may be the impurity particles not readily dissolved in milk powder, can block up
The sense channel for filling in test paper, causes testing result error.
Against the above technical problems, the present invention provides a kind of test strips that can detect urea and melamine simultaneously, described
Test strips are divided into urea detection part and melamine detection part.The test strips can be detected simultaneously and be added in milk powder or milk
Urea and melamine reduce sample dosage to improve the detection efficiency of harmful substance in milk powder or milk, shorten detection
Time, while the impurity particle not readily dissolved in milk powder is filtered, the accuracy of detection is improved, it is easy to operate, it is suitable in common family
Large-scale promotion in front yard.
Summary of the invention
The object of the present invention is to provide the test strips that one kind can detect urea and melamine simultaneously, which can be mentioned
The detection efficiency of harmful substance in high milk powder or milk reduces sample dosage, shortens detection time, while filtering in milk powder and being not easy
Dissolved impurity particle improves the accuracy of detection.
The present invention specifically provides a kind of test strips that can detect urea and melamine simultaneously, which is characterized in that the examination
Paper slip is divided into urea detection part and melamine detection part；Wherein, urea detection part be from top to bottom followed successively by baffle 6,
Filter layer 7 and reagent layer 8, set that there are two circular holes mutually indepedent, arranged in parallel, respectively sample holes 61 and peep hole on baffle
62,62 face filter layer 7 of peep hole has an onesize circular hole 71, and peep hole 62 is made to lead directly to reagent layer 8；Melamine test section
It is divided into lateral chromatographic test paper, is made of bottom plate 1, sample pad 2, bonding pad 3, reacting pad 4 and 5 five part of water absorption pad, wherein instead
Detection band 41 and control band 42 should be coated on pad 4；Urea detection part is tightly fastened by reagent layer 8 and is connected to melamine
In the sample pad 2 of detection part.
Wherein, the baffle 6 is made of opaque plastic material；Filter layer 7 is made of poly (ether sulfone) film material.
Filter layer 7 can filter the impurity particle not readily dissolved in milk powder.
Detection project of the invention includes two detection parts of urea and melamine.
The detection process of urea: when test sample reaches reagent layer 8 by filter layer 7, urea contained therein can be with examination
Be coated in oxidant layer 8 dimethylaminobenzaldehyde reaction generate lemon yellow to dimethylaminoformaldehyde urea.
The detection process of melamine: melamine detection part can be entered by the test sample of urea detection part
Sample pad 2, then carry out the detection process of melamine.Melamine detection part is by bottom plate 1, sample pad 2, bonding pad 3, anti-
Should pad 4 and 5 five part of water absorption pad composition, which be designed and assemble using competition law principle,
Detection band 41 and control band 42 are wherein coated on reacting pad 4, it is anti-to be coated with selenium mark anti-melamine monoclonal on bonding pad 3
Body, detection band 41 are coated with melamine coating comlete antigen melamine-bovine serum albumin(BSA) conjugate (MEL-BSA).Inspection
During survey, the melamine in test sample is with the MEL-BSA competition on detection band 41, with selenium mark anti-melamine monoclonal
Antibody combines colour developing.
The beneficial effects of the present invention are:
1, the present invention can detect the urea and melamine added in milk powder or milk simultaneously, effectively improve milk powder or milk
The detection efficiency of harmful substance in liquid reduces sample and detection reagent dosage, shortens detection time, while reducing cost, is suitable for
The large-scale promotion in average family.
2, in the present invention, the use of filter layer 7 can filter the impurity particle not readily dissolved in milk powder, prevent its obstruction
Channel, to improve the accuracy in detection of urea and melamine.
3, mutually independent circular hole, respectively sample holes there are two in the present invention, being set on the baffle of urea detection part
61 and peep hole 62,62 face filter layer 7 of peep hole have an onesize circular hole 71, peep hole 62 made to lead directly to reagent layer 8, can be with
It avoids filter layer 7 is possible from blocking, more easily observes the detection effect of urea in sample liquid.
Detailed description of the invention
Fig. 1 is main view
1 bottom plate, 2 sample pads, 3 bonding pads, 4 reacting pads and 5 water absorption pads, wherein be coated on 4 reacting pads 41 detection bands and
42 control bands, 6 baffles, 61 sample holes and 62 peep holes, 7 filter layers, 62 peep hole face, 7 filter layer have an onesize circular hole
71,8 reagent layers.
Fig. 2 is top view
The present invention is illustrated by the following examples, but is not limited only to the content in embodiment.
Specific embodiment
The assembling and use of 1 test strips of embodiment
A kind of test strips that can detect urea and melamine simultaneously, the test strips are divided into urea detection part and trimerization
Cyanamide detection part, urea detection part are from top to bottom followed successively by baffle 6, filter layer 7 and reagent layer 8, set on baffle there are two
Independently of each other, circular hole arranged in parallel, respectively sample holes 61 and peep hole 62,62 face filter layer 7 of peep hole have one same
Big small sircle hole 71, makes peep hole lead directly to reagent layer 8；Melamine detection part is lateral chromatographic test paper, is by bottom plate 1, sample
Pad 2, bonding pad 3, reacting pad 4 and 5 five part of water absorption pad composition, are wherein coated with detection band 41 and control band 42 on reacting pad 4；
Urea detection part is tightly fastened in the sample pad 2 for being connected to melamine detection part by reagent layer 8.The baffle 6 is adopted
It is made of opaque plastic material；Filter layer 7 is made of poly (ether sulfone) film material.
In use, milk powder to be measured is sufficiently dissolved with distilled water, appropriate sample liquid is taken, is added dropwise to test paper from sample holes 61.It is quiet
It sets 2 minutes, is observed.
(1) reagent layer 8 is checked from peep hole 62, show lemon yellow when not observing reagent layer 8, be then free of in test sample
Or contain the urea within sensitivity；When observing that reagent layer 8 shows lemon yellow, then containing more than sensitivity in test sample
Urea.
(2) the lateral chromatograph test strip of melamine detection part is observed:
When the control band 42 of test strips and detection band 41 develop the colors, then be free of in test sample or contain sensitivity with
Interior melamine；When the control band 42 of test strips develops the color, and detects band 41 and do not develop the color, then contain sensitivity in test sample
Above melamine；Whether no matter detecting the colour developing of band 41, as long as control band 42 does not develop the color, test strips are no longer valid.
Verification test: the milk powder or milk for being added to urea and melamine are detected, and simultaneously using the test strips with nothing
The milk powder or milk of urea and melamine as negative control, what which can accurately detect to add in milk powder or milk
Urea and melamine, negative control is without detection.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410115323.5A CN104950107B (en) | 2014-03-25 | 2014-03-25 | A kind of test strips that can detect urea and melamine simultaneously |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410115323.5A CN104950107B (en) | 2014-03-25 | 2014-03-25 | A kind of test strips that can detect urea and melamine simultaneously |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104950107A CN104950107A (en) | 2015-09-30 |
| CN104950107B true CN104950107B (en) | 2019-05-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410115323.5A Expired - Fee Related CN104950107B (en) | 2014-03-25 | 2014-03-25 | A kind of test strips that can detect urea and melamine simultaneously |
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| CN (1) | CN104950107B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112540169A (en) * | 2019-09-20 | 2021-03-23 | 艾康生物技术(杭州)有限公司 | Test paper, test card and test method for fecal occult blood |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203053976U (en) * | 2012-12-28 | 2013-07-10 | 闫志文 | Dry chemical multi-item detection device |
| CN103575913A (en) * | 2013-11-18 | 2014-02-12 | 无锡博慧斯生物医药科技有限公司 | Urine analysis and test card for urine microalbumin/urine creatinine |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201522494U (en) * | 2009-04-03 | 2010-07-07 | 天津中新科炬生物制药有限公司 | Duplex occult blood fast diagnositic and chromatographic test paper |
| CN101598727B (en) * | 2009-07-09 | 2012-10-10 | 上海科华生物工程股份有限公司 | Dry chemistry test paper for quantitative determination of urea content in human blood |
| CN201540288U (en) * | 2009-07-09 | 2010-08-04 | 上海科华生物工程股份有限公司 | Dry chemical test paper for quantitatively measuring content of urea of body blood |
| CN201607444U (en) * | 2010-02-08 | 2010-10-13 | 天津中新科炬生物制药有限公司 | Chromatograph test paper for fast diagnosing occult blood |
| CN201845016U (en) * | 2010-10-27 | 2011-05-25 | 江苏出入境检验检疫局动植物与食品检测中心 | Melamine rapid detection test strip |
| CN202141724U (en) * | 2011-07-12 | 2012-02-08 | 英科新创(厦门)科技有限公司 | Joint detection card for blood screening |
| CN202486141U (en) * | 2011-11-11 | 2012-10-10 | 北京勤邦生物技术有限公司 | Reagent kit for detecting beta-lactam antibiotic and melamine |
| CN202383144U (en) * | 2011-12-29 | 2012-08-15 | 北京沙屏研科技有限公司 | Rapid melamine testing box |
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2014
- 2014-03-25 CN CN201410115323.5A patent/CN104950107B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203053976U (en) * | 2012-12-28 | 2013-07-10 | 闫志文 | Dry chemical multi-item detection device |
| CN103575913A (en) * | 2013-11-18 | 2014-02-12 | 无锡博慧斯生物医药科技有限公司 | Urine analysis and test card for urine microalbumin/urine creatinine |
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| CN104950107A (en) | 2015-09-30 |
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Effective date of registration: 20170724 Address after: 730050, No. 247, Jia Zhuang, Qilihe District, Gansu, Lanzhou Applicant after: Yang Tongyue Address before: 730050 science and Technology Park, Lanzhou science and Engineering University, Gansu Applicant before: LANZHOU HONGCHONG BIOLOGICAL ENGINEERING CO.,LTD. |
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