CN104905321A - Wall-breaking Haematoccoccus Pluvialis powder microcapsules and preparation process thereof - Google Patents

Abstract

The invention discloses a process for preparing microcapsules of Haematococcus pluvialis powder, which comprises: step 1, breaking the wall of Haematococcus pluvialls powder; step 2, dissolving sugar and protein in water, and mixing the water with the The ratio of the total amount of sugar and protein is 1:0.2~0.4, then adjust the pH of the aqueous solution of sugar and protein to 6-9, and then react at a temperature of 95-135°C for 0.5-6h to cause Maillard reaction of sugar and protein ; wherein, the sugar is any one of glucose, lactose or maltose, and the protein is casein or soybean protein isolate; and, step 3, using the Maillard reaction product obtained in step 2 as the wall material, the Broken Haematococcus pluvialis powder was used as the core material to prepare microcapsules of broken Haematococcus pluvialls powder. The invention also discloses a broken wall Haematococcus pluvialis powder microcapsule. The microcapsules are high in astaxanthin. It has good stability and water solubility, increasing its application range in liquid food.

Description

A kind of broken-wall Haematococcus pluvialis powder microcapsule and its preparation process

technical field

The invention relates to the technical field of microcapsules, in particular to a preparation process of broken-wall Haematococcus pluvialis powder microcapsules, and also to a broken-wall Haematococcus pluvialls powder microcapsule.

Background technique

Haematoccoccus pluvialis is a widely distributed single-celled green algae, which can form thick-walled spores and accumulate a large number of carotenoids with physiological functions under adverse conditions, mainly including astaxanthin, astaxanthin, and astaxanthin. Astaxanthin monoester, astaxanthin diester, lutein, β-carotene, echinenone, canthaxanthin, etc., 80% of which are astaxanthin and its esters. Haematococcus pluvialis is currently known to exist in nature with the highest content of astaxanthin, up to 1.5-4% dry weight.

Astaxanthin has the highest antioxidant activity due to the carbonyl and hydroxyl groups on its terminal ring, and its antioxidant capacity is 10 times that of β-carotene, 60 times that of proanthocyanidins, and 550 times that of vitamin E (MikiW., Biological functions and activities of animal carotenoids. Pure Appl. Chem., 1991, Vol.63, No.1, pp.141-146). In addition, astaxanthin has a variety of physiological effects such as inhibiting tumor formation, enhancing immune function, and resisting ultraviolet rays (Pashkow FJ, Watumull DG, Campbell CL. Astaxanthin: a novel potential treatment for oxidative stress and inflammation in cardiovascular disease.Am J Cardiol.2008May22; 101 (10A): 58D-68D.), thus widely used in food, medicine, cosmetics, health products, aquaculture and other fields. In the United States, it has been approved by the FDA, allowing it to enter the health care product market as a new dietary ingredient. Super strong antioxidant capacity, various biological activities, and bright red color endow astaxanthin with broad application prospects and market potential. It has been widely used in aquatic products, poultry farming, food, health care products, cosmetics and pharmaceutical industries and other fields.

For the breaking of the wall of Haematococcus pluvialis, or the extraction method of astaxanthin in Haematococcus pluvialls, or the microencapsulation and embedding treatment of astaxanthin oil, there are separate reports in the literature at present, and astaxanthin oil is microencapsulated Chemical embedding requires the extraction of astaxanthin, the process is complicated and the yield and effect will be lost, and the efficacy of Haematococcus pluvialis cannot be fully exerted. At present, there is no report of direct microencapsulation of Haematococcus pluvialis after breaking the wall. Mainly due to the following problems: the cell wall fragments of broken Haematococcus pluvialis increase the difficulty of microencapsulation; conventional modified starch, gum arabic and other wall materials have poor embedding effects, resulting in poor product stability, and directly for the broken wall The spraying of Haematococcus pluvialis liquid will cause the oxidation loss of astaxanthin oil.

Contents of the invention

An object of the present invention is to solve at least the above problems and/or disadvantages and to provide at least the advantages as will be described hereinafter.

Another object of the present invention is to provide a preparation process of broken-walled Haematococcus pluvialis powder microcapsules. The present invention effectively overcomes the problem of broken-walled Pluvialls by using the Maillard reaction product as a wall material and using a spray method in combination with the spraying method. To solve the problem that the cell wall fragments of Haematococcus are difficult to microencapsulate, directly microencapsulate the broken algal powder, combine the microencapsulated astaxanthin with the drying of the algal powder, and produce the microencapsulated astaxanthin containing the algal powder product, the product extraction process is reduced, and the Maillard reaction product is used as the wall material to avoid the reduction of astaxanthin content and the oxidation loss of astaxanthin oil.

Another object of the present invention is to provide a microcapsule of broken-walled Haematococcus pluvialls powder. The broken-walled Haematococcus pluvialls powder microcapsule of the present invention effectively reduces the surface astaxanthin content of the microcapsules and covers the astaxanthin The fishy smell of vegetable oil has good stability and water solubility, the content of astaxanthin reaches 0.5-1%, and the product quality is high.

For this reason, the technical scheme provided by the invention is:

A preparation process of broken-wall Haematococcus pluvialis powder microcapsules, comprising the steps of:

Step 1, the Haematococcus pluvialis powder to be treated is subjected to wall-breaking treatment;

Step 2, dissolving sugar and protein in water at a weight ratio of 1: 0.5 to 3, wherein the ratio of water to the total amount of sugar and protein is 1: 0.2 to 0.4, and then adjusting the aqueous solution of sugar and protein to a pH of 6 -9, and then react at a temperature of 95-135°C for 0.5-6h to cause Maillard reaction of sugar and protein;

Wherein, the sugar is any one of glucose, lactose, or maltose, and the protein is casein or soybean protein isolate; and,

Step 3, using the Maillard reaction product obtained in step 2 as the wall material and the broken-wall Haematococcus pluvialis powder obtained in step 1 as the core material to prepare microcapsules of broken-wall Haematococcus pluvialls powder.

Preferably, in the preparation process of the broken-walled Haematococcus pluvialis powder microcapsules, in the second step, the specific conditions for the Maillard reaction to occur are: the weight ratio of the sugar to the protein is 1 : 1, adjust the aqueous solution of sugar and protein to pH 8.5, and then react at a reaction temperature of 105°C for 2.5h.

Preferably, in the preparation process of the broken-walled Haematococcus pluvialis powder microcapsules, in the third step, the core material and the wall material are prepared into broken-walled Haematococcus pluvialls powder by using a spray drying method The microcapsules, wherein the air inlet temperature is 150-200°C and the air outlet temperature is 60-100°C during spray drying.

Preferably, in the preparation process of the broken-walled Haematococcus pluvialis powder microcapsules, in the third step, the core material and the wall material are prepared into broken-walled Haematococcus pluvialls powder by using a spray drying method Before the microcapsules, the following steps are also included:

(3.1) Mixing of core material and wall material: take the core material obtained in step 1 and the wall material obtained in step 2 in a weight ratio of 1: 2.5-4 and mix them uniformly; and,

(3.2) Homogenization: Under the high-pressure homogenization pressure of 30-50MP, the mixed solution of the core material and the wall material obtained in the step (3.1) is homogenized for 2-3 times.

Preferably, in the preparation process of the described broken-walled Haematococcus pluvialls powder microcapsules, in the step 1, any one of the following methods is adopted to carry out the wall-breaking treatment of the Haematococcus pluvialls powder to be treated :

(1) Mechanical wall breaking of high-pressure homogenizer: homogenize at a temperature of 4°C and a pressure of 40-150MP;

(2) Mechanical wall breaking of ball mill: treatment at 400-600rpm for 1-2h; or,

(3) First, pretreat Haematococcus pluvialis powder at a temperature of 30-60° C. by enzymatic method for 1 hour, and the enzymes used are pectinase and fiber whose total amount accounts for 0.5-2‰ of the weight of said Haematococcus pluvialls powder Sulfase and protease, then add HCl or citric acid for heat treatment for 30-60min, or ball mill for 60-120min.

More preferably, in the preparation process of the broken-walled Haematococcus pluvialis powder microcapsules, in the step 1, in the method (3), the volume percentage of the HCl is 1% to 5%, The mass percentage of the citric acid is 1%-5%.

More preferably, in the preparation process of the described broken-walled Haematococcus pluvialls powder microcapsules, in the step 1, before performing the wall-breaking treatment, add the described hematococcus pluvialls to the Haematococcus pluvialls powder 0.1-2% of the weight of algae powder is an antioxidant, wherein the antioxidant is selected from one or more of VC, VE or BHT.

More preferably, in the preparation process of the broken-walled Haematococcus pluvialis powder microcapsules, the air inlet temperature during the spray drying is 180°C, and the air outlet temperature is 80°C.

More preferably, in the preparation process of the described broken-walled Haematococcus pluvialis powder microcapsules, in the step (3.1), the weight ratio of the core material and the wall material is 1: 2.5; the step ( In 3.2), the pressure of the high-pressure homogenization is 40MP, and the number of homogenization is 2 times.

A broken-wall Haematococcus pluvialis powder microcapsule, which is prepared by any one of the methods described.

The present invention at least includes the following beneficial effects:

The present invention utilizes the Maillard reactant as the main wall material to prepare broken-wall Haematococcus pluvialls microcapsules, and the prepared broken-walled Haematococcus pluvialls microcapsules are red powder. According to the optimized spray drying process of the present invention, the microcapsules Both the embedding rate and yield rate reach over 90%, the astaxanthin content reaches 0.5-1%, has good stability and water solubility, and increases its application range in liquid food.

In the present invention, an antioxidant is added in the wall-breaking process, and the anti-oxidation wall material of the Maillard reactant is used in conjunction with a double-layer protection effect on the astaxanthin.

Adopting the wall material of the present invention and optimized process parameters, such as mixing and homogenization, can effectively reduce the astaxanthin content on the surface of the microcapsules, cover up the fishy smell of astaxanthin oil, and improve product quality.

The invention has the advantages of simple process, high product yield and low production cost.

Other advantages, objectives and features of the present invention will partly be embodied through the following descriptions, and partly will be understood by those skilled in the art through the study and practice of the present invention.

Description of drawings

Fig. 1 is the electron micrograph of the unbroken Haematococcus pluvialis cell observed by electron microscope in one of the embodiments of the present invention;

Fig. 2 is the electron micrograph of the Haematococcus pluvialis cell after the wall breaking observed by the electron microscope in one of the embodiments of the present invention;

Fig. 3 is a scanning electron micrograph of microencapsulated embedded Haematococcus pluvialis powder in one embodiment of the present invention.

Detailed ways

The present invention will be further described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.

It should be understood that terms such as "having", "comprising" and "including" as used herein do not entail the presence or addition of one or more other elements or combinations thereof.

The invention provides a preparation process of broken-wall Haematococcus pluvialis powder microcapsules, comprising the following steps:

Step 1, the Haematococcus pluvialis powder to be treated is subjected to wall-breaking treatment;

Step 2, dissolving sugar and protein in water at a weight ratio of 1: 0.5 to 3, wherein the ratio of water to the total amount of sugar and protein is 1: 0.2 to 0.4, and then adjusting the aqueous solution of sugar and protein to a pH of 6 -9, and then react at a temperature of 95-135°C for 0.5-6h to cause Maillard reaction of sugar and protein;

Wherein, the sugar is any one of glucose, lactose, or maltose, and the protein is casein or soybean protein isolate; and,

Step 3, using the Maillard reaction product obtained in step 2 as the wall material and the broken-wall Haematococcus pluvialis powder obtained in step 1 as the core material to prepare microcapsules of broken-wall Haematococcus pluvialls powder.

Maillard reaction products (Maillard Reaction Products, MRPs) have certain antioxidant properties, and the antioxidant strength of some of them can be compared with the antioxidants commonly used in food; the reaction of different substrates can form different flavor substances , giving the product new flavor characteristics. At the same time, the Maillard reactant has excellent emulsifying and film-forming properties as a wall material. Microencapsulation can effectively reduce the influence of astaxanthin by light, oxygen, and water, and reduce oxidation; prevent volatilization of the core material during storage; protect and control the release of the core material; change the color, shape, density, and dispersion properties of the core material and other physical and chemical properties; reduce or cover up the special fishy smell in astaxanthin oil; improve the mixing and fluidity with other materials, etc. Therefore, the water-soluble Maillard reaction product is used as the wall material to microencapsulate astaxanthin, which can effectively improve the stability and expand its application range. The broken Haematococcus pluvialis microcapsules prepared by the invention are red powder. According to the method of the invention, the microcapsule has good stability and water solubility, which increases its application range in liquid food. The invention has the advantages of simple process, high product yield and low production cost.

As a preference, in the step 2, the specific conditions for the Maillard reaction to occur are: the weight ratio of the sugar and protein is 1:1, the aqueous solution of sugar and protein is adjusted to pH 8.5, and then the reaction temperature Reaction at 105°C for 2.5h.

As a preference, in the third step, the core material and the wall material are prepared into microcapsules of Haematococcus pluvialis powder by spray drying, wherein the temperature of the air inlet during spray drying is 150-200°C, and the temperature of the air outlet is The temperature is 60-100°C. The preparation of microcapsules by spray drying method is mainly to spray and dry the emulsion formed by the core material and the wall material in the drying tower. During the spraying process, although the temperature of the hot air is high, the temperature of the core material is guaranteed to be lower than 100°C due to the rapid evaporation of water from the wall material, so it is especially suitable for drying materials with strong heat sensitivity. In the present invention, the Maillard reaction product is used as the wall material in combination with the spray drying method, so that the embedding rate and yield of the microcapsules prepared according to the present invention can reach more than 90%, and the content of astaxanthin can reach 0.5-1%. 1 g of the broken-wall Haematococcus pluvialis powder microcapsule prepared by the invention is placed in 10-20 mL of water, stirred for 2-5 minutes, and can be quickly dissolved uniformly to obtain a broken-wall Haematococcus pluvialls powder microcapsule solution. That is, the broken-wall Haematococcus pluvialis powder microcapsule has good stability and water solubility, which increases its application range in liquid food.

Preferably, the air inlet temperature during the spray drying is 180°C, and the outlet air temperature is 80°C.

As a preference, in the step three, before the core material and the wall material are prepared into broken-wall Haematococcus pluvialis powder microcapsules by spray drying method, the following steps are also included:

(3.1) Mixing of core material and wall material: take the core material obtained in step 1 and the wall material obtained in step 2 in a weight ratio of 1: 2.5 to 4, the total solids concentration is 15-25%, and fully mix and stir for 5 -30min, make it mix; the total solids include mixed protein, polysaccharide and its reactants, and Haematococcus pluvialis, etc. And, (3.2) homogenization: under the high-pressure homogenization pressure of 30-50MP, the mixed solution of the core material and the wall material obtained in the step (3.1) is homogenized for 2-3 times. Then enter the spray drying step. Through the above steps, the astaxanthin content on the surface of the microcapsules can be effectively reduced, the fishy smell of the astaxanthin oil is covered, and the product quality is improved.

As a preference, in the step (3.1), the weight ratio of the core material and the wall material is 1:2.5, the total solids concentration is 20%, fully stirred for 10 minutes to make it evenly mixed; in the step (3.2), the The pressure of the high-pressure homogenization is 40MP, and the number of homogenization is 2 times.

As preferably, in said step 1, any one of the following methods is adopted to carry out wall-breaking treatment of Haematococcus pluvialis powder to be treated:

(1) Mechanical wall breaking of high-pressure homogenizer: homogenize at a temperature of 4°C and a pressure of 40-150MP;

(2) Mechanical wall breaking of ball mill: treatment at 400-600rpm for 1-2h; or,

(3) First, pretreat Haematococcus pluvialis powder at a temperature of 30-60° C. by enzymatic method for 1 hour, and the enzymes used are pectinase and fiber whose total amount accounts for 0.5-2‰ of the weight of said Haematococcus pluvialls powder Sulfase and protease, then add HCl or citric acid for heat treatment for 30-60min, or ball mill for 60-120min. As shown in Figure 1, Figure 2 and Figure 3, after the above method of breaking the wall, the breaking rate of Haematococcus pluvialis powder can reach more than 90%. Generally speaking, as long as the breaking rate reaches 80-100% It can be used to make microcapsules. Evaluation of wall breaking rate: counting under a plankton counting plate under a microscope,

Preferably, in the step 1, in the method (3), the volume percentage of the HCl is 1%-5%, and the mass percentage of the citric acid is 1%-5%.

As a preference, in the step 1, before the wall breaking treatment, 0.1-2% of the weight of the Haematococcus pluvialis powder is added to the Haematococcus pluvialis powder, wherein the antioxidant is selected from VC, One or more of VE or BHT. Adding antioxidants in the wall-breaking process, combined with Maillard reactant antioxidant wall materials, has a double-layer protection effect on astaxanthin.

A broken-wall Haematococcus pluvialis powder microcapsule, which is prepared by any one of the methods described.

Example 1

Preparation process of broken-wall Haematococcus pluvialis powder microcapsules:

Prepare an 8% aqueous solution of Haematococcus pluvialis powder, add 0.1% of the dry weight of the algae powder as an antioxidant such as vitamin C-sodium, and use a high-pressure homogenizer to mechanically break the wall to obtain a wall-breaking algae with a breaking rate of more than 90%. powder solution as core material;

Prepare the wall material of the Maillard reaction product, the ratio of sugar and protein is 1:1, the pH is 8.5, the reaction temperature is 110°C, and the reaction time is 2.5h;

Mix the core material and wall material thoroughly for 5-10 minutes; high pressure homogenization, homogenization pressure 30-50MP, homogenization 2 times; finally spray drying.

Before the wall material is mixed with the core material, pass through a 100-mesh sieve; the mass ratio of the core material and the wall material is 1:3, and the total solids concentration is 20%; the air inlet temperature of the spray drying is 180°C , the outlet air temperature is 80°C.

Example 2

Preparation process of broken-wall Haematococcus pluvialis powder microcapsules:

Prepare a 10% aqueous solution of Haematococcus pluvialis powder, add an antioxidant such as vitamin E of the dry weight of the algal powder, and adopt a ball mill mechanical wall breaking method to obtain a broken wall algae powder solution with a wall breaking rate of more than 90%; prepare Maillard The wall material of the reaction product, the ratio of sugar and protein is 1:0.8, pH8.0, the reaction temperature is 110°C, and the reaction time is 3.5h; the core material and wall material are mixed and stirred thoroughly for 5-10min; high pressure homogenization, homogenization pressure 30-50MP, homogenize twice; finally spray dry.

Before the wall material is mixed with the core material, pass through a 100-mesh sieve; the mass ratio of the core material and the wall material is 1:4, and the total solids concentration is 18%; the air inlet temperature of the spray drying is 180°C , the outlet air temperature is 80°C.

Example 3

The preparation process of broken-wall Haematococcus pluvialis powder microcapsules comprises the following steps:

Step 1: dissolving Haematococcus pluvialis powder in water to form an aqueous solution, and then adding 0.1% of the weight of the Haematococcus pluvialls powder to antioxidants VC and VE. Then use a high-pressure homogenizer to homogenize at a temperature of 4°C and a pressure of 40MP, and mechanically break the wall to obtain broken-wall Haematococcus pluvialis powder as a core material.

Step 2, dissolve sugar and protein in water at a weight ratio of 1:0.5, wherein the ratio of water to the total amount of sugar and protein is 1:0.2, then adjust the aqueous solution of sugar and protein to a pH of 6, and then React at 95°C for 6 hours to cause Maillard reaction between sugar and protein, and use the reaction product as wall material;

Wherein, the sugar is glucose, and the protein is casein; and,

Step 3, using the Maillard reaction product obtained in step 2 as the wall material, and the broken-wall Haematococcus pluvialis powder obtained in step 1 as the core material, and prepare broken-wall Haematococcus pluvialls powder microcapsules according to the following steps :

(3.1) Mixing of core material and wall material: take the core material obtained in step 1 and the wall material obtained in step 2 in a weight ratio of 1:3 and mix them; and,

(3.2) Homogenization: Under the high-pressure homogenization pressure of 30 MP, the mixed solution of the core material and the wall material obtained in the step (3.1) was homogenized for 3 times.

(3.3) Prepare the mixed solution of the homogenized core material and wall material into broken-wall Haematococcus pluvialis powder microcapsules by spray drying method, wherein the air inlet temperature is 150°C during spray drying, and the air outlet temperature is 150°C. is 60°C.

Example 4

The preparation process of broken-wall Haematococcus pluvialis powder microcapsules comprises the following steps:

Step 1: dissolving Haematococcus pluvialis powder in water to form an aqueous solution, and then adding VC, an antioxidant accounting for 1% by weight of the Haematococcus pluvialls powder, thereinto. After that, firstly, the enzyme method is used to pretreat the Haematococcus pluvialis powder at a temperature of 30-60°C for 1 hour. and protease, and then add HCl or citric acid for heat treatment for 30-60min, or perform ball milling treatment for 60-120min. The volume percentage of the HCl is 1%-5%, and the mass percentage of the citric acid is 1%-5%. The broken wall is used to obtain the broken Haematococcus pluvialis powder as the core material.

Step 2, dissolve sugar and protein in water at a weight ratio of 1:1, wherein the ratio of water to the total amount of sugar and protein is 1:0.3, then adjust the aqueous solution of sugar and protein to pH 8.5, and then React at 105°C for 2.5 hours to cause Maillard reaction between sugar and protein, and use the reaction product as wall material;

Wherein, the sugar is maltose, and the protein is casein; and,

Step 3, using the Maillard reaction product obtained in step 2 as the wall material, and the broken-wall Haematococcus pluvialis powder obtained in step 1 as the core material, and prepare broken-wall Haematococcus pluvialls powder microcapsules according to the following steps :

(3.1) Mixing of core material and wall material: take the core material obtained in step 1 and the wall material obtained in step 2 in a weight ratio of 1:2.5 and mix them; and,

(3.2) Homogenization: Under the high-pressure homogenization pressure of 40MP, the mixed solution of the core material and the wall material obtained in step (3.1) was homogenized twice.

(3.3) Prepare the mixed solution of the homogenized core material and wall material into broken-wall Haematococcus pluvialis powder microcapsules by spray drying method, wherein the air inlet temperature is 180°C during spray drying, and the air outlet temperature is 180°C. is 80°C.

Example 5

The preparation process of broken-wall Haematococcus pluvialis powder microcapsules comprises the following steps:

Step 1, dissolving the Haematococcus pluvialis powder in water to form an aqueous solution, and then adding 2% of the weight of the Haematococcus pluvialls powder to the antioxidant BHT, wherein the antioxidant is selected from VC, VE or BHT one or more of. After that, use a ball mill to mechanically break the wall: process at a rotational speed of 400-600rpm for 1-2 hours to mechanically break the wall, and obtain the broken Haematococcus pluvialis powder as the core material.

Step 2, dissolving sugar and protein in water with a weight ratio of 1: 3, wherein the ratio of water to the total amount of sugar and protein is 1: 0.4, then adjusting the aqueous solution of sugar and protein to pH to be 9, and then React at 135°C for 0.5h to cause Maillard reaction between sugar and protein, and use the reaction product as wall material;

Wherein, the sugar is lactose, and the protein is soybean protein isolate; and,

Step 3, using the Maillard reaction product obtained in step 2 as the wall material, and the broken-wall Haematococcus pluvialis powder obtained in step 1 as the core material, and prepare broken-wall Haematococcus pluvialls powder microcapsules according to the following steps :

(3.1) Mixing of core material and wall material: take the core material obtained in step 1 and the wall material obtained in step 2 in a weight ratio of 1:4 and mix them; and,

(3.2) Homogenization: Under the high-pressure homogenization pressure of 50MP, the mixed solution of the core material and the wall material obtained in the step (3.1) was homogenized twice.

(3.3) Prepare the mixed solution of the homogenized core material and wall material into broken-wall Haematococcus pluvialis powder microcapsules by spray drying method, wherein the air inlet temperature is 200°C during spray drying, and the air outlet temperature is 200°C. is 100°C.

The present invention effectively overcomes the problem that the cell wall fragments of broken Haematococcus pluvialis are difficult to microencapsulate by using the Maillard reaction product as the wall material in combination with the spraying method, and directly microencapsulates the broken wall algae powder, Combine microencapsulated astaxanthin with algal powder drying to produce microencapsulated astaxanthin products containing algal powder, which reduces the extraction process of the product, and uses Maillard reaction products as wall materials to avoid the content of astaxanthin Reduce and avoid the oxidation loss of astaxanthin oil. This process combines the sealing effect of microencapsulation and the antioxidant effect of the Maillard reaction product itself, and has a strong effect of protecting astaxanthin. The microcapsules prepared according to the present invention have an embedding rate and yield of more than 90%, an astaxanthin content of 0.5-1%, good stability and water solubility, and increase its application range in liquid food .

Although the embodiment of the present invention has been disclosed as above, it is not limited to the use listed in the specification and implementation, it can be applied to various fields suitable for the present invention, and it can be easily understood by those skilled in the art Therefore, the invention is not limited to the specific details and examples shown and described herein without departing from the general concept defined by the claims and their equivalents.

Claims (10)

1. a preparation technology for broken wall haematococcus pluvialis powder capsule, is characterized in that, comprises the steps:

Step one, broken wall treatment is carried out to pending haematococcus pluvialis powder;

Step 2, with part by weight 1: 0.5 ~ 3 get sugar and protein soluble in water, wherein the ratio of the total amount of water and described sugar and protein is 1: 0.2 ~ 0.4, the aqueous solution regulating sugar and protein is afterwards 6-9 to pH, at temperature 95-135 DEG C, react 0.5-6h again, make sugar and protein generation Maillard reaction;

Wherein, described sugar is any one in glucose, lactose or maltose, and described protein is casein or soybean protein isolate; And,

The broken wall haematococcus pluvialis powder that step 3, the maillard reaction product obtained in step 2 obtain in wall material, step one prepares broken wall haematococcus pluvialis powder capsule as core.

2. broken wall haematococcus pluvialis powder microcapsule preparation process as claimed in claim 1, it is characterized in that, in described step 2, the actual conditions that described Maillard reaction occurs is: the part by weight of described sugar and protein is 1: 1, the aqueous solution regulating sugar and protein is 8.5 to pH, then at reaction temperature 105 DEG C, reacts 2.5h.

3. the preparation technology of broken wall haematococcus pluvialis powder capsule as claimed in claim 1, it is characterized in that, in described step 3, utilize spray drying process that described core and wall material are prepared into broken wall haematococcus pluvialis powder capsule, wherein, during spraying dry, EAT is 150-200 DEG C, and leaving air temp is 60-100 DEG C.

4. the preparation technology of broken wall haematococcus pluvialis powder capsule as claimed in claim 3, is characterized in that, in described step 3, utilizes before described core and wall material be prepared into broken wall haematococcus pluvialis powder capsule by spray drying process, also comprises the steps:

(3.1) core wall material mixing: be 1: 2.5 ~ 4 get the wall material that obtains in the core and step 2 obtained in step one and mix by weight proportion; And,

(3.2) homogeneous: under high-pressure homogeneous pressure 30-50MP, carries out homogeneous 2 ~ 3 times to the mixed solution of the core obtained in step (3.1) and wall material.

5. the preparation technology of the broken wall haematococcus pluvialis powder capsule as described in as arbitrary in Claims 1-4, is characterized in that, in described step one, adopts any one method in following methods to carry out broken wall treatment to pending haematococcus pluvialis powder:

(1) high pressure homogenizer mechanical breaking-wall method: in temperature 4 DEG C, carry out homogeneous under pressure 40-150MP;

(2) ball mill mechanical breaking-wall method: process 1-2h under rotating speed 400-600rpm; Or,

(3) first enzyme process haematococcus pluvialis powder 1h under temperature 30 ~ 60 DEG C of pretreatment is adopted, the enzyme adopted is that total amount accounts for the pectase of described haematococcus pluvialis powder weight 0.5 ~ 2 ‰, cellulase and protease, add HCl more afterwards or citric acid carries out heating 30-60min, or carry out ball-milling treatment 60-120min.

6. the preparation technology of broken wall haematococcus pluvialis powder capsule as claimed in claim 5, it is characterized in that, in described step one, in described method (3), the percent by volume of described HCl is 1% ~ 5%, and the mass percent of described citric acid is 1% ~ 5%.

7. broken wall haematococcus pluvialis powder microcapsule preparation process as claimed in claim 5, it is characterized in that, in described step one, carry out adding the antioxidant accounting for described haematococcus pluvialis powder weight 0.1-2% in the forward direction haematococcus pluvialis powder of broken wall treatment, wherein said antioxidant is selected from one or more in VC, VE or BHT.

8. the preparation technology of broken wall haematococcus pluvialis powder capsule as claimed in claim 3, it is characterized in that, EAT during described spraying dry is 180 DEG C, and leaving air temp is 80 DEG C.

9. the preparation technology of broken wall haematococcus pluvialis powder capsule as claimed in claim 4, it is characterized in that, in described step (3.1), the part by weight of described core and wall material is 1: 2.5; In described step (3.2), described high-pressure homogeneous pressure is under 40MP, and the number of times of homogeneous is 2 times.

10. a broken wall haematococcus pluvialis powder capsule, is characterized in that, described broken wall haematococcus pluvialis powder capsule is obtained by the method as described in as arbitrary in claim 1 to 9.