CN104880553A - Kit for detecting residual ivermectin in beef - Google Patents
Kit for detecting residual ivermectin in beef Download PDFInfo
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- 229960002418 ivermectin Drugs 0.000 title claims abstract description 38
- AZSNMRSAGSSBNP-UHFFFAOYSA-N 22,23-dihydroavermectin B1a Natural products C1CC(C)C(C(C)CC)OC21OC(CC=C(C)C(OC1OC(C)C(OC3OC(C)C(O)C(OC)C3)C(OC)C1)C(C)C=CC=C1C3(C(C(=O)O4)C=C(C)C(O)C3OC1)O)CC4C2 AZSNMRSAGSSBNP-UHFFFAOYSA-N 0.000 title claims abstract description 26
- SPBDXSGPUHCETR-JFUDTMANSA-N 8883yp2r6d Chemical compound O1[C@@H](C)[C@H](O)[C@@H](OC)C[C@@H]1O[C@@H]1[C@@H](OC)C[C@H](O[C@@H]2C(=C/C[C@@H]3C[C@@H](C[C@@]4(O[C@@H]([C@@H](C)CC4)C(C)C)O3)OC(=O)[C@@H]3C=C(C)[C@@H](O)[C@H]4OC\C([C@@]34O)=C/C=C/[C@@H]2C)/C)O[C@H]1C.C1C[C@H](C)[C@@H]([C@@H](C)CC)O[C@@]21O[C@H](C\C=C(C)\[C@@H](O[C@@H]1O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](OC)C3)[C@@H](OC)C1)[C@@H](C)\C=C\C=C/1[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\1)O)C[C@H]4C2 SPBDXSGPUHCETR-JFUDTMANSA-N 0.000 title claims abstract description 24
- 235000015278 beef Nutrition 0.000 title claims abstract description 12
- 239000012528 membrane Substances 0.000 claims abstract description 29
- 238000004458 analytical method Methods 0.000 claims abstract description 22
- 238000012360 testing method Methods 0.000 claims abstract description 20
- 238000001514 detection method Methods 0.000 claims abstract description 16
- 239000000853 adhesive Substances 0.000 claims abstract description 11
- 239000002245 particle Substances 0.000 claims abstract description 11
- 230000001070 adhesive effect Effects 0.000 claims abstract description 10
- 238000012546 transfer Methods 0.000 claims abstract description 9
- 238000003908 quality control method Methods 0.000 claims abstract description 6
- 239000000463 material Substances 0.000 claims abstract description 5
- 241000283707 Capra Species 0.000 claims description 5
- 239000000020 Nitrocellulose Substances 0.000 claims description 3
- 229920001220 nitrocellulos Polymers 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 3
- -1 water-absorbing pad Substances 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 9
- 238000004020 luminiscence type Methods 0.000 abstract description 5
- 239000002250 absorbent Substances 0.000 abstract description 4
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 238000012216 screening Methods 0.000 description 4
- 238000002965 ELISA Methods 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- IBSREHMXUMOFBB-JFUDTMANSA-N 5u8924t11h Chemical compound O1[C@@H](C)[C@H](O)[C@@H](OC)C[C@@H]1O[C@@H]1[C@@H](OC)C[C@H](O[C@@H]2C(=C/C[C@@H]3C[C@@H](C[C@@]4(O3)C=C[C@H](C)[C@@H](C(C)C)O4)OC(=O)[C@@H]3C=C(C)[C@@H](O)[C@H]4OC\C([C@@]34O)=C/C=C/[C@@H]2C)/C)O[C@H]1C.C1=C[C@H](C)[C@@H]([C@@H](C)CC)O[C@]11O[C@H](C\C=C(C)\[C@@H](O[C@@H]2O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](OC)C3)[C@@H](OC)C2)[C@@H](C)\C=C\C=C/2[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\2)O)C[C@H]4C1 IBSREHMXUMOFBB-JFUDTMANSA-N 0.000 description 1
- 239000005660 Abamectin Substances 0.000 description 1
- 241000238421 Arthropoda Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 229950008167 abamectin Drugs 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000003096 antiparasitic agent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000003120 macrolide antibiotic agent Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000001846 repelling effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
本发明公开了一种检测牛肉中伊维菌素残留的试剂盒，包括盒式外壳及位于盒体外壳内部的免疫层析试纸条，免疫层析试纸条包括样品垫、分析膜、吸水垫、粘性底衬及包被有上转发光材料颗粒标记的抗伊维菌素单抗的结合垫，粘性底衬胶合固定在盒式外壳的底部，分析膜胶合在粘性底衬上，结合垫及吸水垫分别胶合在分析膜的两侧，样品垫胶合在结合垫远离分析膜的一侧，在分析膜上设置有包被抗伊维菌素单抗的检测带及包被羊抗鼠lgG的质控带，盒式外壳上设置有分别与样品垫、分析膜及吸水垫对应的样品添加孔、结果读取窗口及终点指示窗口。本发明采用上转发光技术与免疫技术结合，适宜在现场的快速检测，成本低，具有准确、快速、定量检测的特点。
The invention discloses a test kit for detecting ivermectin residues in beef, which comprises a box-type casing and an immunochromatographic test strip located inside the casing of the box body. The immunochromatographic test strip includes a sample pad, an analysis membrane, a water-absorbent Pad, adhesive backing and binding pad coated with anti-ivermectin monoclonal antibody labeled with up-transfer luminescent material particles, the adhesive backing is glued and fixed on the bottom of the cassette shell, the analysis membrane is glued on the adhesive backing, the binding pad and water-absorbing pads are glued on both sides of the analysis membrane respectively, and the sample pad is glued on the side of the binding pad away from the analysis membrane. The quality control belt is equipped with a sample addition hole, a result reading window and an end point indication window corresponding to the sample pad, analysis membrane and water-absorbing pad respectively on the cassette shell. The present invention adopts the combination of up-conversion luminescence technology and immune technology, is suitable for rapid detection on site, has low cost, and has the characteristics of accurate, rapid and quantitative detection.
Description
技术领域 technical field
本发明涉及医疗技术领域，具体涉及一种检测牛肉中伊维菌素残留的试剂盒。 The invention relates to the field of medical technology, in particular to a kit for detecting ivermectin residues in beef.
背景技术 Background technique
伊维菌素是一种半合成大环内酯类抗生素，其毒性大大低于阿维菌素，为目前兽医临床上使用最多的抗寄生虫药，对体内外寄生虫特别是线虫和节肢动物均有良好的驱杀作用，广泛地使用于猪、牛、羊等家畜中。伊维菌素具有生殖毒性，且在动物体内残留时间长，长期暴露对人体具有严重健康损害危险，且对水生环境有剧毒性。 Ivermectin is a semi-synthetic macrolide antibiotic, its toxicity is much lower than that of abamectin, and it is currently the most widely used antiparasitic drug in veterinary medicine, especially for internal and external parasites, especially nematodes and arthropods All have good repelling and killing effects, and are widely used in pigs, cattle, sheep and other livestock. Ivermectin is toxic to reproduction and remains in animals for a long time. Long-term exposure has the risk of serious health damage to humans and is highly toxic to the aquatic environment.
目前对伊维菌素的检测方法主要有高效液相色谱法（HPLC）、液质联用法(LC-MS)、酶联免疫法等（ELISA）。但HPLC和LC-MS法的样品前处理复杂，操作繁琐，而且这两种方法所需的仪器昂贵，成本高，不适于对大量样本的筛查。而ELISA法易出现假阳性，造成结果的偏差。 The current detection methods for ivermectin mainly include high performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS), and enzyme-linked immunoassay (ELISA). However, the sample pretreatment of HPLC and LC-MS methods is complicated and the operation is cumbersome, and the instruments required by these two methods are expensive and costly, so they are not suitable for screening a large number of samples. The ELISA method is prone to false positives, resulting in biased results.
发明内容 Contents of the invention
为了解决上述技术问题，本发明提供一种检测牛肉中伊维菌素残留的试剂盒，该试剂盒采用上转发光技术与免疫技术结合，适宜在现场的快速检测，成本低，适于对大量样本的筛查，具有准确、快速、定量检测的特点。 In order to solve the above technical problems, the present invention provides a kit for detecting ivermectin residues in beef. The kit adopts the combination of up-conversion luminescence technology and immune technology, and is suitable for rapid detection on the spot, with low cost, and is suitable for a large number of The screening of samples has the characteristics of accurate, rapid and quantitative detection.
本发明的目的通过以下技术方案来具体实现： The purpose of the present invention is specifically achieved through the following technical solutions:
一种检测牛肉中伊维菌素残留的试剂盒，包括盒式外壳及位于盒体外壳内部的免疫层析试纸条，所述免疫层析试纸条包括样品垫、分析膜、吸水垫、粘性底衬及包被有上转发光材料颗粒标记的抗伊维菌素单抗的结合垫，所述粘性底衬胶合固定在盒式外壳的底部，分析膜胶合在粘性底衬上，结合垫及吸水垫分别胶合在分析膜的两侧，所述样品垫胶合在结合垫远离分析膜的一侧，在分析膜上设置有包被抗伊维菌素单抗的检测带及包被羊抗鼠lgG的质控带，所述盒式外壳上设置有分别与样品垫、分析膜及吸水垫对应的样品添加孔、结果读取窗口及终点指示窗口。 A test kit for detecting ivermectin residues in beef, comprising a box-type casing and an immunochromatographic test strip located inside the casing of the box body, the immunochromatographic test strip including a sample pad, an analysis membrane, an absorbent pad, An adhesive backing and a binding pad coated with an anti-ivermectin monoclonal antibody labeled with up-transfer luminescent material particles, the adhesive backing is glued and fixed on the bottom of the cassette housing, the analysis membrane is glued on the adhesive backing, and the binding pad and water-absorbing pads are glued on both sides of the analysis membrane respectively, and the sample pad is glued on the side away from the analysis membrane of the binding pad. A quality control belt for mouse IgG, the cassette housing is provided with sample addition holes corresponding to the sample pad, analysis membrane and water-absorbent pad, a result reading window and an end point indicating window.
进一步的，所述分析膜为硝酸纤维素膜。 Further, the analysis membrane is a nitrocellulose membrane.
进一步的，所述盒体外壳由透明材料制成。 Further, the box shell is made of transparent material.
本发明一种检测牛肉中伊维菌素残留的试剂盒，该试剂盒的结合垫上包被有上转发光材料颗粒标记的抗伊维菌素单抗，同时在分析膜上设置有包被抗伊维菌素单抗的检测带及包被羊抗鼠lgG的质控带，反应时样品中伊维菌素与两株抗体反应结合，在检测带形成伊维菌素蛋白抗体-伊维菌素蛋白抗原-上转发光颗粒标记的伊维菌素蛋白抗体复合物，上转发光颗粒在红外光激发下发射可见光，发射光的强度与样品中伊维菌素蛋白的浓度成正比。无论样品中是否存在伊维菌素，在质控带都会形成羊抗鼠-上转发光颗粒标记的抗伊维菌素抗体复合物，利用上转发光免疫分析仪对试纸条进行扫描分析，显示测量结果。本试剂盒采用上转发光技术与免疫技术结合，适宜在现场的快速检测，成本低，适于对大量样本的筛查，具有准确、快速、定量检测的特点。 The invention is a kit for detecting ivermectin residues in beef. The binding pad of the kit is coated with an anti-ivermectin monoclonal antibody labeled with up-transfer luminescent material particles, and at the same time, an anti-ivermectin monoclonal antibody coated with The detection zone of ivermectin monoclonal antibody and the quality control zone coated with goat anti-mouse IgG, the ivermectin in the sample reacts with the two antibodies during the reaction, and forms an antibody to ivermectin protein in the detection zone-Ivermectin Protein antigen - ivermectin protein antibody complex labeled with up-transfer luminescent particles, the up-transfer luminescent particles emit visible light under the excitation of infrared light, and the intensity of the emitted light is proportional to the concentration of ivermectin protein in the sample. Regardless of whether there is ivermectin in the sample, a goat anti-mouse-anti-ivermectin antibody complex labeled with upturned luminescent particles will be formed in the quality control zone, and the test strip will be scanned and analyzed by an upturned luminescent immunoassay analyzer. Displays the measurement results. This kit adopts the combination of up-transfer luminescence technology and immunological technology, which is suitable for rapid detection on the spot, low cost, suitable for screening a large number of samples, and has the characteristics of accurate, rapid and quantitative detection.
附图说明 Description of drawings
下面根据附图和实施例对本发明作进一步详细说明。 The present invention will be described in further detail below according to the drawings and embodiments.
图1是本发明一种检测牛肉中伊维菌素残留的试剂盒的立体图； Fig. 1 is a perspective view of a test kit for detecting ivermectin residues in beef of the present invention;
图2是免疫层析试纸条的结构示意图。 Fig. 2 is a structural schematic diagram of an immunochromatographic test strip.
图中：1-盒式外壳；2-样品垫；3-分析膜；4-吸水垫；5-粘性底衬；6-结合垫；7-检测带；8-质控带；9-样品添加孔；10-结果读取窗口；11-终点指示窗口。 In the figure: 1-cassette shell; 2-sample pad; 3-analytical membrane; 4-absorbent pad; 5-adhesive backing; 6-binding pad; 7-detection strip; Well; 10-result reading window; 11-end point indication window.
具体实施方式 Detailed ways
如图1-2所示，本发明实施例所述的一种检测牛肉中伊维菌素残留的试剂盒，包括盒式外壳1及位于盒体外壳1内部的免疫层析试纸条，免疫层析试纸条包括样品垫2、分析膜3、吸水垫4、粘性底衬5及包被有上转发光材料颗粒标记的抗伊维菌素单抗的结合垫6，粘性底衬5胶合固定在盒式外壳1的底部，分析膜3胶合在粘性底衬5上，结合垫6及吸水垫4分别胶合在分析膜3的两侧，样品垫2胶合在结合垫6远离分析膜3的一侧，在分析膜3上设置有包被抗伊维菌素单抗的检测带7及包被羊抗鼠lgG的质控带8，盒式外壳1上设置有分别与样品垫2、分析膜3及吸水垫4对应的样品添加孔9、结果读取窗口10及终点指示窗口11。 As shown in Figures 1-2, a test kit for detecting ivermectin residues in beef described in the embodiment of the present invention includes a box-type shell 1 and an immunochromatographic test strip positioned inside the box shell 1, and the immunochromatography test strip is located inside the box shell 1. The chromatography test strip includes a sample pad 2, an analysis membrane 3, a water-absorbing pad 4, an adhesive backing 5 and a binding pad 6 coated with anti-ivermectin monoclonal antibody marked with up-transfer luminescent material particles, and the adhesive backing 5 is glued together It is fixed on the bottom of the box-type housing 1, the analysis membrane 3 is glued on the adhesive backing 5, the binding pad 6 and the water-absorbing pad 4 are glued on both sides of the analysis membrane 3 respectively, and the sample pad 2 is glued on the side of the binding pad 6 away from the analysis membrane 3. On one side, a detection strip 7 coated with anti-ivermectin monoclonal antibody and a quality control strip 8 coated with goat anti-mouse IgG are arranged on the analysis membrane 3, and the cassette shell 1 is provided with the sample pad 2 and the analysis strip respectively. The membrane 3 and the water-absorbing pad 4 correspond to the sample addition hole 9 , the result reading window 10 and the end point indication window 11 .
一种优选的实施方式，分析膜3为硝酸纤维素膜。 In a preferred embodiment, the analytical membrane 3 is a nitrocellulose membrane.
优选的，盒体外壳1由透明材料制成，便于观测。 Preferably, the box shell 1 is made of transparent material for easy observation.
本发明一种检测牛肉中伊维菌素残留的试剂盒，在使用时，添加样品中的伊维菌素与两株抗体反应结合，在检测带形成伊维菌素蛋白抗体-伊维菌素蛋白抗原-上转发光颗粒标记的伊维菌素蛋白抗体复合物，上转发光颗粒在红外光激发下发射可见光，发射光的强度与样品中伊维菌素蛋白的浓度成正比。无论样品中是否存在伊维菌素，在质控带都会形成羊抗鼠-上转发光颗粒标记的抗伊维菌素抗体复合物。利用上转发光免疫分析仪对试纸条进行扫描分析，显示测量结果，在整个检测过程中，通过虹吸作用提供液体流过整个试纸条的动力。 The present invention is a kit for detecting ivermectin residues in beef. When in use, the ivermectin in the sample is added to react with two strains of antibodies, and the ivermectin protein antibody-ivermectin is formed in the detection zone Protein antigen - ivermectin protein antibody complex labeled with uptransfer luminescent particles. The uptransfer luminescent particles emit visible light under the excitation of infrared light, and the intensity of the emitted light is proportional to the concentration of ivermectin protein in the sample. Regardless of the presence or absence of ivermectin in the sample, a goat anti-mouse-anti-ivermectin antibody complex labeled with up-conversion luminescent particles will be formed in the control zone. The test strip is scanned and analyzed by an upturning luminescence immunoassay analyzer, and the measurement result is displayed. During the whole detection process, the power of the liquid flowing through the entire test strip is provided by the siphon effect.
本试剂盒采用上转发光技术与免疫技术结合，适宜在现场的快速检测，成本低，适于对大量样本的筛查，具有准确、快速、定量检测的特点。 This kit adopts the combination of up-transfer luminescence technology and immunological technology, which is suitable for rapid detection on the spot, low cost, suitable for screening a large number of samples, and has the characteristics of accurate, rapid and quantitative detection.
最后应说明的是：以上所述仅为本发明的优选实施例而已，并不用于限制本发明，对于本领域的技术人员来说，其依然可以对前述各实施例所记载的技术方案进行调节，或者对其中部分技术特征进行等同替换，凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。 Finally, it should be noted that the above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. For those skilled in the art, they can still adjust the technical solutions described in the foregoing embodiments. , or equivalent replacement of some of the technical features, any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.
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| CN1690711A (en) * | 2004-04-23 | 2005-11-02 | 中国人民解放军军事医学科学院微生物流行病研究所 | Immunochromatographic test strip based on up-conversion luminescence technology |
| CN1811440A (en) * | 2006-02-17 | 2006-08-02 | 中国农业大学 | Method for detecting Ivermectin and special enzyme-linked immune reagent kit thereof |
| CN202330436U (en) * | 2011-11-08 | 2012-07-11 | 黑龙江八一农垦大学 | Selenium marker test paper for detecting Avermectin residues |
| CN103675287A (en) * | 2013-12-12 | 2014-03-26 | 洛阳莱普生信息科技有限公司 | Ivermectin chemiluminescence enzyme-linked immunoassay rapid detection kit |
| CN204631044U (en) * | 2015-06-02 | 2015-09-09 | 中国农业科学院兰州畜牧与兽药研究所 | A kind ofly detect the residual kit of ivermectin in beef |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1690711A (en) * | 2004-04-23 | 2005-11-02 | 中国人民解放军军事医学科学院微生物流行病研究所 | Immunochromatographic test strip based on up-conversion luminescence technology |
| CN1811440A (en) * | 2006-02-17 | 2006-08-02 | 中国农业大学 | Method for detecting Ivermectin and special enzyme-linked immune reagent kit thereof |
| CN202330436U (en) * | 2011-11-08 | 2012-07-11 | 黑龙江八一农垦大学 | Selenium marker test paper for detecting Avermectin residues |
| CN103675287A (en) * | 2013-12-12 | 2014-03-26 | 洛阳莱普生信息科技有限公司 | Ivermectin chemiluminescence enzyme-linked immunoassay rapid detection kit |
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Application publication date: 20150902 |