CN104873479A - Collagen polypeptide skin membrane capable of reducing swelling and relieving itching and preparation method of collagen polypeptide skin membrane - Google Patents

Abstract

The invention relates to a preparation method of a collagen polypeptide skin detumescence and antipruritic film, belonging to the field of biomedical materials. The collagen polypeptide skin detumescence and antipruritic film of the present invention is based on collagen polypeptide, sodium carboxymethyl cellulose, dialdehyde starch, starch, forms the basic skeleton structure of film, adds glycerol as humectant, controls the humidity of film, and then Active ingredients such as Scutellaria baicalensis extract, Coptis chinensis extract, Phellodendron phellodendron extract, and rhubarb extract are added. Finally, the uniformly mixed paste mixture is poured into a mold for molding, and dried in an oven at a constant temperature. The collagen polypeptide skin detumescence and antipruritic film with good biocompatibility can be obtained by peeling off the film.

Description

Collagen polypeptide skin detumescence and antipruritic film and preparation method thereof

technical field

The invention relates to a collagen polypeptide skin detumescence and antipruritic film and a preparation method thereof, belonging to the field of biomedical materials.

Background technique

When a mosquito bites, it will release its saliva and toxins into the human blood. Because of its certain antigenicity, it will cause local redness, swelling and itching at the bite site. and relieve itching. Some people are sensitive to mosquito bites and have stronger reactions. Essential oils can relieve itching and reduce swelling against mosquito bites. It is mainly composed of menthol, eucalyptus oil, clove powder, camphor, essential oils, etc. It has the effect of refreshing, repelling mosquitoes and relieving itching. But if it is aimed at viruses or fungi carried by mosquitoes, the above ingredients will not have any effect. Refreshing oil, also known as panacea oil, has similar effects and ingredients to essential oils. Its main components are menthol, peppermint oil, camphor oil, camphor, eucalyptus oil, clove oil, etc., and it also contains a very small amount of ammonia water. It can be seen that, for mosquito bites, wind balm and cooling oil mainly play a role of cooling and cooling, refreshing the brain, relieving itching and reducing swelling, but cannot play the therapeutic role of antibacterial and anti-inflammatory. Soapy water is slightly alkaline, which can neutralize the acidic toxins of mosquitoes, and has a certain effect on reducing swelling and itching of the skin.

Traditional Chinese medicine often adopts the method of topical smearing and sticking in the treatment of skin diseases. This method is accepted by patients because of its ready-to-use, definite curative effect, and easy portability. This method has become an important means of skin disease treatment. A very important idea in the treatment principles of traditional Chinese medicine is to reconcile yin and yang, and acid-base neutralization is also a kind of yin-yang reconciliation. Scutellaria baicalensis, Coptis chinensis, Phellodendron Phellodendron, these traditional Chinese medicines for clearing heat and detoxifying, can reduce swelling and relieve itching of the skin. The main component of Scutellaria baicalensis, baicalin, has antibacterial, antiviral, and anti-inflammatory effects; coupled with rhubarb, it is even better in reducing swelling, stopping bleeding and detoxifying. Of course, what is more important is the synergistic effect of the active ingredients of the above four traditional Chinese medicines, making it achieve the therapeutic effects of relieving itching, reducing swelling, antibacterial and anti-inflammatory.

Chinese medicine film preparation is an emerging field in Chinese medicine preparations, including active ingredients of Chinese medicine and film-forming materials as carriers of Chinese medicine. There are two types of film-forming materials: natural and synthetic polymers. The film-forming materials are required to be non-toxic, non-irritating, stable in nature, ineffective with drugs, not affecting drug efficacy, good film-forming performance, rich in sources, The price is cheap and so on. With the development of science and technology, especially the development of biotechnology in recent years, the research, development and use of biological products are increasing day by day. The main component of the dermis is collagen, and collagen contains a lot of amino acids, so it has good hydrophilicity. Collagen film is the hotspot of current research. The reason is that type Ⅰ collagen is the main structural protein that constitutes the skin. Collagen rebuilt through self-polymerization and cross-linking can form a film-forming material with certain strength and stability. This material has high affinity. Water-based, non-toxic, good biocompatibility, degradability and other biological characteristics. Since the bio-collagen film is not an organic compound, it is completely composed of collagen and has no toxic side effects. In addition, the biological collagen film also has the quality requirements and drug loading function of the film-forming material.

Contents of the invention

The invention uses the dialdehyde starch to cross-link the collagen polypeptide and the like into a network macromolecular material. The more the cross-linking dose is added, the denser the network structure will be, and the drug in it will diffuse out into the solution or the small molecules in the solution will experience greater resistance to permeate from the surface of the membrane to the inside of the membrane, so the release behavior of diffusion control It will become weaker with the increase of the degree of cross-linking, and the release rate will be more gentle. The release behavior of Scutellaria baicalensis extract, Coptis chinensis extract, Phellodendron phellodendron extract, and rhubarb extract in the drug film is in a diffusion-controlled manner. Therefore, the release rate of the drug in the drug film can be reasonably controlled by the degree of cross-linking, so that the drug can be better released on the skin and provide good conditions for skin recovery. This material will play an important role in the treatment of skin detumescence and antipruritic, and this product increases the diversity of skin detumescence and antipruritic materials.

The purpose of the present invention is to provide a new collagen polypeptide skin swelling and antipruritic film, through the release of Scutellaria baicalensis extract, Coptis chinensis extract, Phellodendron phellodendron extract, and rhubarb extract to achieve the effect of promoting skin detumescence and antipruritic, to solve mosquito bite problem,

The collagen polypeptide skin detumescence and antipruritic film of the invention has good biocompatibility and air permeability. After repeated moistening with sodium bicarbonate solution before external application, the effect of reducing swelling and relieving itching is better. the

The collagen polypeptide skin detumescence and antipruritic film of the present invention is a film made of raw materials with the following weight ratio:

Collagen peptide 0.1~1.5 parts

Glycerol 0.6~5.0 parts

Dialdehyde starch 0.1-2.0 parts

Starch 0.1~1.0 parts

Sodium carboxymethylcellulose 0.2~3.0 parts

Deionized water 5.0~40.0 parts

Scutellaria baicalensis extract 0.1~1.8 parts

Coptis chinensis extract 0.1~1.5 parts

Phellodendron Phellodendri Extract 0.1~2.0 parts

Rhubarb extract 0.1~2.2 parts

In the collagen polypeptide skin detumescence and antipruritic film mentioned above, the collagen polypeptide powder is self-made. Collagen peptides can promote cell regeneration and reduce scars. The active ingredients of traditional Chinese medicine, Scutellaria baicalensis extract, Coptis chinensis extract, Cortex Phellodendri extract, and rhubarb extract have the functions of reducing swelling, relieving itching, and neutralizing venom; the cross-linking agent dialdehyde starch makes The film-forming material is cross-linked to form a network macromolecular structure, which improves the mechanical strength of the film; the plasticizer glycerin endows the film with a certain degree of humidity and toughness. Starch is added as a setting agent for the film. Scutellaria baicalensis extract, Coptis chinensis extract, Phellodendron phellodendron extract, and rhubarb extract are fixed in the skeleton structure, and the drug is released slowly to achieve the purpose of long-lasting drug effect.

The preparation method of the collagen polypeptide skin detumescence and antipruritic film comprises the following steps:

(1) Select fresh pigskin, remove the excess fat on the pigskin by physical methods, wash and cut the pigskin into 3mm ³ ~5mm ³ particles and place them in the reaction bottle. Use the newly prepared Na ₂ CO ₃ solution with a concentration of 10% as a degreasing agent, and add it into the reaction flask until the pigskin particles are submerged. Degrease with constant stirring in a water bath at 45°C for 70 minutes, and filter with suction. Put the filtered pigskin particles into another reaction flask, add distilled water to adjust the pH to 8, and add 6% to 12% of pigskin weight trypsin. Enzyme hydrolyze in a water bath at 40°C for 2-6 hours. Finally, the centrifuge centrifuges to separate the above mixed solution, takes the middle layer solution containing collagen polypeptide, and removes the undegreased fat in the upper layer and the insoluble root hairs and elastic fibers in the lower layer. Then dry in an oven at 40° C. to 60° C., and grind to obtain collagen polypeptide powder.

( 2 ) Put 0.2~3.0 parts by weight of sodium carboxymethyl cellulose and 5.0~39.0 parts by weight of deionized water into a beaker, and stir to form a transparent jelly;

(3) Add 1.0~2.0 parts by weight of dialdehyde starch and 0.6~5.0 parts by weight of glycerol into (2), mix and stir evenly;

(4) 0.1-1.0 parts by weight of starch, 0.1-1.5 parts by weight of collagen polypeptide, 0.1-1.8 parts by weight of Scutellaria baicalensis extract, 0.1-1.5 parts by weight of Coptidis Rhizoma extract, 0.1-2.0 parts by weight of Phellodendron Phellodendri extract, 0.1 ~2.2 parts by weight of rhubarb extract, put into a beaker, add 1.0~9.0 parts by weight of deionized water, stir to make it evenly mixed;

(5) Mix the mixture obtained in (3) and (4) with mechanical stirring to obtain a sticky substance;

(6) Spread the viscous obtained in (5) evenly on a dry and clean template to form a liquid layer with uniform thickness and smooth surface, and dry it in an oven at 55°C to 65°C for a drying time of 0.6 After ~3 hours, remove the film to get the collagen polypeptide skin detumescence and antipruritic film.

Detailed ways

The following examples further illustrate the present invention, and the scope of the present invention is not limited to the following examples, but is limited by the description and claims of the present invention. the

Example 1

In a clean and dry container, add 0.3 parts of collagen peptides, 0.1 parts of starch, 0.3 parts of Scutellaria baicalensis extract, 0.4 parts of Phellodendron phellodendri extract, 0.3 parts of rhubarb extract, 0.4 parts of Coptis chinensis extract, 16.0 parts of deionized water, 0.5 parts Dialdehyde starch, 2.0 parts of glycerol, 0.5 parts of sodium carboxymethylcellulose.

Stir to combine evenly and get a sticky mass.

Spread the viscous evenly on a dry and clean template to form a gel-like layer with uniform thickness and smooth surface, dry it in a constant temperature oven at 60°C, and peel off the film to obtain the collagen polypeptide skin detumescence and antipruritic film.

Example 2

In a clean and dry container, add 0.2 parts of collagen peptides, 0.1 parts of starch, 0.3 parts of Scutellaria baicalensis extract, 0.5 parts of Phellodendron Phellodendri extract, 0.4 parts of rhubarb extract, 0.4 parts of Coptidis Rhizoma extract, 20.5 parts of deionized water, 1.0 parts Dialdehyde starch, 2.2 parts of glycerol, 0.5 parts of sodium carboxymethylcellulose.

Stir to combine evenly and get a sticky mass.

Spread the viscous evenly on a dry and clean template to form a gel-like layer with uniform thickness and smooth surface, dry it in a constant temperature oven at 60°C, and peel off the film to obtain the collagen polypeptide skin detumescence and antipruritic film.

Example 3

In a clean and dry container, add 0.4 parts of collagen peptides, 0.1 parts of starch, 0.5 parts of Scutellaria baicalensis extract, 0.4 parts of Phellodendron Phellodendron extract, 1.8 parts of rhubarb extract, 0.3 part of Coptis chinensis extract, 28.5 parts of deionized water, 1.0 parts of Dialdehyde starch, 2.5 parts of glycerol, 0.5 parts of sodium carboxymethylcellulose.

Stir to combine evenly and get a sticky mass.

Spread the viscous evenly on a dry and clean template to form a gel-like layer with uniform thickness and smooth surface, dry it in a constant temperature oven at 60°C, and peel off the film to obtain the collagen polypeptide skin detumescence and antipruritic film.

Example 4

In a clean and dry container, add 0.3 parts of collagen peptides, 0.1 parts of starch, 0.4 parts of Scutellaria baicalensis extract, 1.5 parts of Phellodendron Phellodendri extract, 0.6 parts of rhubarb extract, 0.4 parts of Coptis chinensis extract, 26.5 parts of deionized water, 1.0 parts Dialdehyde starch, 2.5 parts of glycerol, 0.5 parts of sodium carboxymethylcellulose.

Stir to combine evenly and get a sticky mass.

Spread the viscous evenly on a dry and clean template to form a gel-like layer with uniform thickness and smooth surface, dry it in a constant temperature oven at 60°C, and peel off the film to obtain the collagen polypeptide skin detumescence and antipruritic film.

Example 5

In a clean and dry container, add 0.4 parts of collagen peptides, 0.1 parts of starch, 0.4 parts of Scutellaria baicalensis extract, 0.7 parts of Phellodendron Phellodendri extract, 0.5 parts of rhubarb extract, 1.5 parts of Coptidis Rhizoma extract, 26.5 parts of deionized water, 1.0 parts Dialdehyde starch, 2.5 parts of glycerol, 0.5 parts of sodium carboxymethylcellulose.

Stir to combine evenly and get a sticky mass.

Spread the viscous evenly on a dry and clean template to form a gel-like layer with uniform thickness and smooth surface, dry it in a constant temperature oven at 60°C, and peel off the film to obtain the collagen polypeptide skin detumescence and antipruritic film.

Example 6

In a clean and dry container, add 0.3 parts of collagen peptides, 0.1 parts of starch, 0.5 parts of Scutellaria baicalensis extract, 0.5 parts of Phellodendron Phellodendri extract, 0.4 parts of rhubarb extract, 0.5 parts of Coptis chinensis extract, 20.5 parts of deionized water, 1.0 parts Dialdehyde starch, 2.6 parts of glycerol, 0.5 parts of sodium carboxymethylcellulose.

Stir to combine evenly and get a sticky mass.

Spread the viscous evenly on a dry and clean template to form a gel-like layer with uniform thickness and smooth surface, dry it in a constant temperature oven at 60°C, and peel off the film to obtain the collagen polypeptide skin detumescence and antipruritic film.

Claims (2)

1. a collagen polypeptide skin detumescence and antipruritic film is characterized in that it is the film that is made of the raw material of following weight ratio: Collagen peptide 0.1~1.5 parts Glycerol 0.6~5.0 parts Dialdehyde starch 0.1-2.0 parts Starch 0.1~1.0 parts Sodium carboxymethylcellulose 0.2~3.0 parts Deionized water 5.0~40.0 parts Scutellaria baicalensis extract 0.1~1.8 parts Coptis chinensis extract 0.1~1.5 parts Phellodendron Phellodendri Extract 0.1~2.0 parts Rhubarb extract 0.1~2.2 parts. 2. prepare the method for collagen polypeptide skin detumescence antipruritic film described in claim 1, it is characterized in that the method is made up of the following steps: ( 1 ) Put 0.2~3.0 parts by weight of sodium carboxymethylcellulose and 5.0~39.0 parts by weight of deionized water into a beaker, and stir evenly in a constant temperature water bath at 30°C~50°C to form a transparent jelly; (2) Add 1.0~2.0 parts by weight of dialdehyde starch and 0.6~5.0 parts by weight of glycerol into (1), mix and stir evenly; (3) 0.1-1.0 parts by weight of starch, 0.1-1.5 parts by weight of collagen polypeptide, 0.1-1.8 parts by weight of Scutellaria baicalensis extract, 0.1-1.5 parts by weight of Coptidis Rhizoma extract, 0.1-2.0 parts by weight of Phellodendron Phellodendri extract, 0.1 ~2.2 parts by weight of rhubarb extract, put into a beaker, add 1.0~9.0 parts by weight of deionized water, stir to make it evenly mixed; (4) The mixture obtained in (2) and (3) is mechanically stirred to obtain a viscous substance; (5) Spread the viscous obtained in (4) evenly on a dry and clean template to form a liquid layer with uniform thickness and smooth surface, and dry it in an oven at 55°C to 65°C for a drying time of 0.6 After ~3 hours, remove the film to get the collagen polypeptide skin detumescence and antipruritic film.