CN104849114A - Special slide for comet experiment - Google Patents
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- CN104849114A CN104849114A CN201510243811.9A CN201510243811A CN104849114A CN 104849114 A CN104849114 A CN 104849114A CN 201510243811 A CN201510243811 A CN 201510243811A CN 104849114 A CN104849114 A CN 104849114A
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- 238000002474 experimental method Methods 0.000 title claims abstract description 15
- 239000004793 Polystyrene Substances 0.000 claims abstract description 7
- 229920002223 polystyrene Polymers 0.000 claims abstract description 7
- 239000004033 plastic Substances 0.000 claims abstract description 3
- 239000000758 substrate Substances 0.000 claims description 3
- 239000011521 glass Substances 0.000 abstract description 6
- 239000000463 material Substances 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 3
- 230000004888 barrier function Effects 0.000 description 2
- 230000005778 DNA damage Effects 0.000 description 1
- 231100000277 DNA damage Toxicity 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000003927 comet assay Methods 0.000 description 1
- 231100000170 comet assay Toxicity 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
本发明公开了一种彗星实验专用载片，包括基板和边板，基板为条形平板，基板上面设有2个以上底部下凹且平坦的圆形槽孔；基板的两端设有竖向的边板；基板与边板为由透明聚苯乙烯塑料制成的一体结构。本发明结构简单，使用方便，材质由透明聚苯乙烯代替玻璃，不易与碱液反应，可以反复利用，节约资源。
The invention discloses a special carrier for comet experiments, which comprises a base plate and a side plate. The base plate is a strip-shaped flat plate, and the base plate is provided with more than two circular slot holes with concave and flat bottoms; the two ends of the base plate are provided with vertical The side plate; the base plate and the side plate are an integral structure made of transparent polystyrene plastic. The invention has the advantages of simple structure and convenient use, and the material is made of transparent polystyrene instead of glass, which is not easy to react with lye, can be used repeatedly, and saves resources.
Description
技术领域 technical field
本发明涉及一种彗星实验专用载片。 The invention relates to a slide specially used for comet experiments.
背景技术 Background technique
彗星实验(comet assay)是在单细胞水平上灵敏检测DNA损伤和修复的常用方法。 The comet assay is a common method for sensitive detection of DNA damage and repair at the single-cell level.
当前常用的彗星实验用载片存在以下不足： The currently commonly used slides for comet experiments have the following deficiencies:
1.目前常用载片为玻璃材质，在实验过程中易与碱液反应而给实验结果带来一定影响； 1. At present, the commonly used slides are made of glass, which is easy to react with lye during the experiment and will have a certain impact on the experimental results;
2.目前市场上的玻璃载片仅为2孔、3孔及96孔，槽孔是由疏水屏障中间直接挖孔并贴附在该载玻片上制作而成。铺胶至其未凝固之前，液体凝胶易从上层的输水屏障和下层载玻片之间的空隙溢出所围成的圆形孔，使最终所铺凝胶厚度不够且不均匀，同时可致胶面不平整； 2. The glass slides currently on the market are only 2-well, 3-well and 96-well. The slots are made by directly digging holes in the middle of the hydrophobic barrier and attaching them to the slide. Before the gel is laid until it is not solidified, the liquid gel is easy to overflow from the gap between the upper water barrier and the lower glass slide to form a circular hole, so that the thickness of the final gel is not enough and uneven. Cause the glue surface to be uneven;
3.普通载玻片为片状，不方便取放； 3. Ordinary glass slides are sheet-shaped, which is inconvenient to take and place;
4.目前市场上彗星实验用载片价格相对昂贵，且为一次性使用，普适性不强，而且浪费资源，造成环境污染，不适应当前节约型社会的发展。 4. At present, slides for comet experiments are relatively expensive in the market, and they are only for one-time use. They are not universal, waste resources, cause environmental pollution, and are not suitable for the development of the current conservation-oriented society.
发明内容 Contents of the invention
本发明要解决的技术问题是提供一种彗星实验专用载片。 The technical problem to be solved by the present invention is to provide a special slide for comet experiments.
为了解决上述技术问题，本发明采用的技术方案是：彗星实验专用载片，包括基板和边板，基板为条形平板，基板上面设有2个以上底部下凹且平坦的圆形槽孔；基板的两端设有竖向的边板；基板与边板为由透明聚苯乙烯塑料制成的一体结构。 In order to solve the above-mentioned technical problems, the technical solution adopted in the present invention is: a special carrier for comet experiments, including a base plate and side plates, the base plate is a strip-shaped flat plate, and the base plate is provided with more than two concave and flat circular slots at the bottom; Both ends of the base plate are provided with vertical side plates; the base plate and the side plates are an integral structure made of transparent polystyrene plastic.
作为优选，圆形槽孔为4个。 Preferably, there are four circular slots.
作为优选，基板厚度为0.9 mm。 Preferably, the thickness of the substrate is 0.9 mm.
作为优选，圆形槽孔的半径为10 mm，圆形槽孔下凹的深度为0.6 mm。 Preferably, the radius of the circular slot is 10 mm, and the depth of the circular slot is 0.6 mm.
作为优选，边板的高度为10 mm。 Preferably, the height of the side plate is 10 mm.
本发明的有益效果是： The beneficial effects of the present invention are:
结构简单，使用方便，材质由透明聚苯乙烯代替玻璃，不易与碱液反应，可以反复利用，节约资源。 The structure is simple, easy to use, and the material is made of transparent polystyrene instead of glass, which is not easy to react with lye, can be used repeatedly, and saves resources.
附图说明 Description of drawings
下面结合附图和具体实施方式对本发明作进一步详细的说明。 The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.
图1是本发明彗星实验专用载片实施例的结构示意图。 Fig. 1 is a schematic structural view of an embodiment of a special slide for comet experiments of the present invention.
图中，1-基板，2-边板，3-圆形槽孔。 In the figure, 1-base plate, 2-side plate, 3-circular slot.
具体实施方式 Detailed ways
图1是一种用于彗星实验的专用4孔载片，由基板1和边板2构成。 FIG. 1 is a special 4-hole slide used in comet experiments, which consists of a base plate 1 and a side plate 2 .
基板1为长条形的平板，在平板的两端设有竖向设置的边板2。 The base plate 1 is a strip-shaped flat plate, and vertical side plates 2 are arranged at both ends of the flat plate.
基板的上面挖有4个底部向下内凹的圆形槽孔3，槽孔的底部平坦，圆形槽孔的半径为10 mm，下凹深度为0.6 mm。槽孔的间距2 mm。 Four circular slots 3 with bottoms concave downwards are dug on the substrate. The bottoms of the slots are flat, the radius of the circular slots is 10 mm, and the depth of the depression is 0.6 mm. The spacing of the slots is 2 mm.
平板的长度为8.40cm，宽度为3.28cm，平板厚度为0.9 mm，槽孔底部的厚度只有0.3 mm。两端的边板高度为10 mm。 The length of the plate is 8.40 cm, the width is 3.28 cm, the thickness of the plate is 0.9 mm, and the thickness of the bottom of the slot is only 0.3 mm. The height of the side plates at both ends is 10 mm.
基板和边板采用透明聚苯乙烯制成一体结构。 The base and side panels are of one-piece construction in clear polystyrene.
在使用时，将槽孔内加入凝胶，并使得凝胶底部铺设平整，厚度适中，即可使用。 When in use, add gel into the slot, make the bottom of the gel lay flat, and the thickness is moderate, then it can be used.
与市场上产品相比，本实施例具有如下特点： Compared with products on the market, this embodiment has the following characteristics:
1.材质采用聚苯乙烯，不易与碱液反应； 1. The material is polystyrene, which is not easy to react with lye;
2.在基板上一体挖出的圆形槽孔，使凝胶被封锁在该孔槽内，不易溢漏，且底部平坦，且槽孔深度适中，使得凝胶胶面铺设平整，厚度合适。 2. The circular slot hole dug integrally on the base plate makes the gel be blocked in the slot, which is not easy to spill, and the bottom is flat, and the slot depth is moderate, so that the gel surface is laid flat and the thickness is appropriate.
3.该板具有4个孔槽，可同时进行4个样品的处理，解决由于处理时间不同而导致的试验结果差异，并填补市场上2孔、3孔及96孔载片的空缺； 3. The plate has 4 wells, which can process 4 samples at the same time, solve the difference in test results caused by different processing time, and fill the vacancy of 2-well, 3-well and 96-well slides in the market;
4.两端设有竖直的边板，方便取放； 4. There are vertical side panels at both ends for easy access;
5. 该种载片价格低廉，极易推广；且可以反复利用，节约资源。 5. This type of slide is cheap and easy to promote; it can be used repeatedly to save resources.
以上所述的本发明实施方式，并不构成对本发明保护范围的限定。任何在本发明的精神和原则之内所作的修改、等同替换和改进等，均应包含在本发明的权利要求保护范围之内。 The embodiments of the present invention described above are not intended to limit the protection scope of the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the claims of the present invention.
Claims (5)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510243811.9A CN104849114A (en) | 2015-05-13 | 2015-05-13 | Special slide for comet experiment |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510243811.9A CN104849114A (en) | 2015-05-13 | 2015-05-13 | Special slide for comet experiment |
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| CN104849114A true CN104849114A (en) | 2015-08-19 |
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| CN201510243811.9A Pending CN104849114A (en) | 2015-05-13 | 2015-05-13 | Special slide for comet experiment |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017214822A1 (en) * | 2016-06-13 | 2017-12-21 | 石庆学 | Sample adding plate for single cell gel electrophoresis |
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| CN1435488A (en) * | 2002-02-01 | 2003-08-13 | 先进基因股份有限公司 | Plastic substrate useful for making bio-chip |
| CN1519329A (en) * | 2003-01-24 | 2004-08-11 | 财团法人工业技术研究院 | microarray biochip reactor |
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| CN1818648A (en) * | 2006-03-15 | 2006-08-16 | 北京博奥生物芯片有限责任公司 | Agarose gel plastic substrate, its production and use |
| CN102297858A (en) * | 2010-06-24 | 2011-12-28 | 中国科学院生态环境研究中心 | Test tube method and apparatus for testing SOS / umu genotoxic effect |
| CN102719352A (en) * | 2012-06-06 | 2012-10-10 | 西安交通大学 | Cell chip slide for preparing microarray cell chips and preparation method |
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2015
- 2015-05-13 CN CN201510243811.9A patent/CN104849114A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6174683B1 (en) * | 1999-04-26 | 2001-01-16 | Biocept, Inc. | Method of making biochips and the biochips resulting therefrom |
| FR2793560A1 (en) * | 1999-09-07 | 2000-11-17 | Commissariat Energie Atomique | Analytical support, useful e.g. for immunological or hybridization assays, comprises analytical sites, each associated with a resonant microcavity for emitting light |
| CN1335501A (en) * | 2001-04-27 | 2002-02-13 | 上海晶泰生物技术有限公司 | Multiple-sample microarray biochip |
| CN1343887A (en) * | 2001-08-07 | 2002-04-10 | 东南大学 | Process for preparing antigen microarray based on self antibody repertoire |
| CN1435488A (en) * | 2002-02-01 | 2003-08-13 | 先进基因股份有限公司 | Plastic substrate useful for making bio-chip |
| CN1547668A (en) * | 2002-03-15 | 2004-11-17 | 索尼公司 | Substrate for bioassay, bioassay apparatus, and reading apparatus |
| CN1519329A (en) * | 2003-01-24 | 2004-08-11 | 财团法人工业技术研究院 | microarray biochip reactor |
| CN1771438A (en) * | 2003-04-10 | 2006-05-10 | 库诺公司 | Improved composite microarry slides |
| CN1818648A (en) * | 2006-03-15 | 2006-08-16 | 北京博奥生物芯片有限责任公司 | Agarose gel plastic substrate, its production and use |
| CN102297858A (en) * | 2010-06-24 | 2011-12-28 | 中国科学院生态环境研究中心 | Test tube method and apparatus for testing SOS / umu genotoxic effect |
| CN102719352A (en) * | 2012-06-06 | 2012-10-10 | 西安交通大学 | Cell chip slide for preparing microarray cell chips and preparation method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017214822A1 (en) * | 2016-06-13 | 2017-12-21 | 石庆学 | Sample adding plate for single cell gel electrophoresis |
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Application publication date: 20150819 |
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