CN104770366A - Sterilization composition containing JS399-19 and benzamide - Google Patents

Abstract

The invention discloses a bactericidal composition containing cyclostrobin and cyprostrobin. The mass ratio of the active components cyclostrobin and cyclostrobin is 15:1-1:15. The combined use of cyprostrobin and methazamine in the present invention can reduce dosage, effectively reduce ecological damage and environmental pollution, and improve crop yield and quality; cyprostrobin and methapam in the present invention are both low Equal toxicity, safe for humans, animals, and beneficial organisms; it can delay the resistance of bacteria to single agents. Cyclostrobin and rustamine have different mechanisms of action. Combining the two in a certain proportion has high bactericidal activity and can delay the emergence and development of pathogen resistance. It has high bactericidal activity in actual use and can be used in larger To a certain extent, it avoids the selection pressure on pathogens caused by single agents of cyprostrobin and methazamine alone, delays the emergence and development of drug resistance of pathogens, and reduces the total dosage per unit area, which is economical, efficient and environmentally friendly.

Description

A kind of bactericidal composition containing cyprostrobin and rustamine

technical field

The invention belongs to the field of agricultural fungicides, and in particular relates to a fungicidal composition of cyclostrobin and fenoxam.

Background technique

Cyclostrobin (molecular formula is C ₁₂ H ₁₂ N ₂ O ₂ ), the structural formula is as follows:

Chemical name: ethyl 2-cyano-3-amino-3-phenylphenylacrylate; belongs to the original innovative invention. The compound belongs to cyanoacrylates, has high efficiency, slight toxicity, low residue, and is environmentally friendly. It has protective and therapeutic effects on various plant diseases caused by Fusarium, and can be used to prevent and control wheat head blight caused by Fusarium, Cotton Fusarium Wilt, Banana Panama Disease, Rice Bakanae Disease, Watermelon Fusarium Wilt, etc.

Promethamine (English common name: Benzamide), the molecular formula is C ₁₇ H ₁₉ NO ₂ , and the structural formula is as follows:

The general chemical name is 3'-isopropoxy-2-methylbenzoanilide, a systemic fungicide with special biological activity on basidiomycete fungal diseases, and has special effects on basidiomycete fungi. It can effectively control rice sheath blight, cotton blight, wheat stripe rust, and can also be used to control wheat snow rot, pear red spot, potato and tomato rhizocytosis, pear and chrysanthemum rust, cucumber seedling blight.

Contents of the invention

The invention proposes a novel fungicide composition containing cyprostrobin and rustamine, which is used for preventing and treating various crop diseases such as wheat head blight. Cyclostrobin and cyprostrobin have different structural types and different mechanisms of action. Combining the two in a certain proportion has high bactericidal activity in actual use, and can avoid cyclostrobin and cyclostrobin to a greater extent. The selective pressure on pathogens caused by the single agent of flurustamine used alone can delay the occurrence and development of drug resistance of pathogens.

A bactericidal composition containing cyclostrobin and cyprostrobin according to the present invention comprises active components cyclostrobin and cyclostrobin in a mass ratio of 15:1 to 1:15, preferably with a mass ratio of 10 :1-1:5, more preferably the mass ratio is 5:1-1:1.

Commonly used agricultural fungicide adjuvants are added to the composition to prepare a commonly used agricultural fungicide composition dosage form.

The dosage forms of the bactericidal composition are water suspension concentrate, wettable powder and water dispersible granule. The agricultural fungicide additives used include wetting agents, dispersants, surfactants, thickeners, defoamers, fillers and other additives. The wetting agents and dispersants used can be alkyl naphthalene sulfonate Formaldehyde condensate, sodium dibutylnaphthalene sulfonate formaldehyde condensate, sodium oleic acid methyl amino ethyl sulfonate, sodium lignosulfonate, calcium lignosulfonate, alkylphenol polyoxyethylene ether, alkylphenol One or more of polyoxyethylene phosphate, castor oil polyoxyethylene ether, etc.; the thickener used can be xanthan gum, polyvinyl alcohol, etc.; the defoamer used can be phosphate defoaming One or more of agent and silicone defoamer; the filler used can be one or more of white carbon black, kaolin, attapulgite, etc.;

The active ingredient composed of cyprostrobin and rustamine in the composition accounts for 10%-60% of the total mass of the bactericidal composition.

The fungicidal composition can be used for preventing and controlling crop fungal diseases of wheat head blight.

Through the detailed analysis of the action mechanism and bactericidal properties of cyclostrobin and rustamine, the Wadley method was used to design the experiment and process the results. First measure the EC ₅₀ of two single compounds (hereinafter referred to as medicaments) of cyprostrobin and methazamine, set the combination ratio of the two medicaments according to the Wadley method according to the EC ₅₀ of the two single medicaments, and determine according to the SR value of the Wadley synergy index. The appropriate composition ratio is then verified by Sun Yunpei's method.

The target object of the test is wheat head blight. The test uses the colony diameter method. Take the mother solution of cyprostrobin and methamine, and configure it into a flat plate of the required drug solution, about 14ml per dish, and set a non-drug plate control (CK) at the same time, repeat 3 dishes for each treatment, that is, repeat 3 times. At the edge of the pre-cultured colony, use a sterilized hole puncher to prepare mycelial blocks with a diameter of 5 mm, move them to different medium plates, culture at 28 ° C for 4 days, measure the diameter of the colony (mm), and calculate the growth inhibition rate by the following formula :

The mycelia growth inhibition rate is converted into the inhibition probability value (Y), the concentration of the agent is converted into the concentration logarithm (X), and the virulence regression line is made according to the concentration logarithm as the abscissa and the probability value as the ordinate, to obtain the Toxicity regression equation of ester and rustamine single agent and its mixture against wheat head blight, and calculate EC ₅₀ , EC ₉₀ value and correlation coefficient r value.

According to the Wadley method to evaluate the interaction of the mixture, the calculation formula is as follows:

EC _{50 (theoretical value)} = (a+b)/(a/EC _50a +b/EC _50b ), SR=EC _{50 (theoretical value)} /EC _{50 (actual value)} .

Where a and b are the content ratios of each component in the mixture, and the effect of the mixture is analyzed by SR value. If SR≤0.5, the mixture of the two agents has an antagonistic effect; if SR=0.5～1.5, the mixture of the two agents has an additive effect; if SR≥1.5, the mixture of the two agents has a synergistic effect.

Table 1 The test results of cyprostrobin and rustamine single agent and their mixture against wheat gibberellic virus

From the analysis of the data in Table 1, it can be concluded that when cyprostrobin and proprustamine are mixed at a ratio of 15:1 to 1:15, they can inhibit the growth of wheat head blight mycelium, and they all show an additive effect. Mixed at a ratio of 5:1, it can strongly inhibit the growth of wheat scab mycelium.

The present invention has better control effects on crop fungal diseases such as wheat head blight, and the combined use of the two agents can reduce the dosage, effectively reduce ecological damage and environmental pollution, and can improve crop yield and quality; Both ester and fluramine are of low toxicity, which are safe for humans, animals, and organisms; they can delay the resistance of bacteria to a single agent.

Detailed ways

The following examples are a further description of the content of the present invention as an explanation of the technical content of the present invention, but the essential content of the present invention is not limited to the following examples, those of ordinary skill in the art can and should know any Simple changes or replacements of the essential spirit of the invention shall fall within the scope of protection required by the present invention.

Example 1

The preparation method of 36% cyclostrobin · Rustamine suspension concentrate: by mass percentage.

Preparation method: According to the formula, weigh the above components in proportion, add to a ball mill and mill for 30 minutes, filter, pump into the dispersion tank for 3 minutes at high speed, and then fully grind with a sand mill to control the particle diameter of the solid component within 2 microns , and stir evenly after grinding to obtain a suspension product. 60-80g per mu is used to prevent and control wheat scab, and the effect can reach more than 90%.

Example 2

The preparation method of 32% cyclostrobin · Rustamine suspension concentrate: by mass percentage.

Preparation method, with embodiment 1. 60-80g per mu is used to prevent and control wheat scab, and the effect can reach 75%.

Example 3

The preparation method of 30% cyclostrobin · Rustamine suspension concentrate: by mass percentage.

Preparation method, with embodiment 1. 60-80g per mu is used to prevent and control wheat scab, and the effect can reach 80%.

Example 4

The preparation method of the 42% cyclostrobin · Rustamine wettable powder: by mass percentage.

Preparation method: Add the above components into a mixer according to the formula ratio, mix them evenly, and then carry out airflow crushing. After the crushing is completed, stir well and evenly to get a wettable powder product. 60-80g per mu is used to prevent and control wheat scab, and the effect can reach 86%.

Example 5

The preparation method of 40% cyclostrobin · Rustamine wettable powder: by mass percentage.

Preparation method, with embodiment 4. 60-80g per mu is used to control wheat scab and the effect is about 80%.

Example 6

The preparation method of 10% cyanoxystrobin·methanoxamine water-dispersible granule: by mass percentage.

Preparation method: add the above components into a mixer according to the formula ratio, mix them evenly, and then carry out jet milling. After the milling is completed, add a small amount of purified water to fully stir evenly, extrude and granulate, and dry to obtain the water-dispersible granule product. 60-80g per mu is used to prevent and control wheat scab, and the effect can reach 82%.

Claims (7)

1. a bactericidal composition for 2-cyano-3-amino-3-phenylancryic acetate-containing and mebenil, is characterized in that: include active component 2-cyano-3-amino-3-phenylancryic acetate and mebenil that mass ratio is 15:1 ~ 1:15.

2. bactericidal composition according to claim 1, is characterized in that: the mass ratio of described 2-cyano-3-amino-3-phenylancryic acetate and mebenil is 10:1 ~ 1:5.

3. bactericidal composition according to claim 2, is characterized in that: the mass ratio of described 2-cyano-3-amino-3-phenylancryic acetate and mebenil is 5:1 ~ 1:1.

4. the bactericidal composition according to any one of claim 1-3, is characterized in that: be added with conventional agricultural bactericidal agent aid and make agriculturally conventional bactericidal composition formulation.

5. bactericidal composition according to claim 4, is characterized in that: described bactericidal composition formulation is aqueous suspension agent, wetting powder, water-dispersible granules.

6. bactericidal composition according to claim 4, is characterized in that: the active ingredient of described 2-cyano-3-amino-3-phenylancryic acetate and mebenil composition accounts for 10% ~ 60% of bactericidal composition gross mass.

7. the bactericidal composition described in any one of claim 1-6 is for preventing and treating the crop fungal disease of wheat scab.