CN1047203C - Enzymolytic egg albumen solution as health-care liquid - Google Patents

Abstract

The present invention relates to a method for enzymolysis of egg albumen, and products produced by the method. The egg albumen is treated by enzymolysis by using papain under certain reaction conditions, and then, an egg albumen solution which is treated by enzymolysis is generated after a treating process is finished. The egg albumen solution which is treated by enzymolysis contains amino acids and polypeptides which are needed for human bodies are easy to absorb, and can be used as the raw material of nutrient health care solutions and cosmetics for human bodies.

Description

Enzymically hydrolyse Protalbinic acid solution, preparation method and purposes

The present invention relates to product and method with zymyhydrolyzed protein matter.

Egg is one of daily required food of human body, Protalbinic acid is a kind of zona pellucida viscosity and the fluxion in the egg, every egg on average contains protein 10 grams, fat 0.1 gram, candy 1.2 grams, calcium 0.059 gram, phosphorus 0.016 gram, trace iron and vitamin A, micro-riboflavin, the peptide that the physiology using value is very high, amino acid are formed.It has functions such as the growth of promotion, repairing cell.Protalbinic acid is albuminous a kind of, and albumin is a kind of crucial protein, and its major function is to keep plasma colloid osmotic pressure and increase Q volume of blood.Albumin extensively is present in the serum of animal; for example: bovine serum albumin has been widely used in cell cultures; and human serum albumin is the maximum protein of content in the blood plasma; its content is 3.8-4.8%; be used for the treatment of low albumin disease clinically; the oedema and the ascites that are used for serious hepatic diseases and cause by low albumin; it can also activating enzyme and protective enzyme vigor; about 5 gram albumin liquid just are equivalent to 100ml blood plasma or 250ml whole blood; so it is a kind of more valuable blood product, according to measuring the amino acid composition very similar (table 1) of finding Protalbinic acid and human serum albumin.The Protalbinic acid molecular weight is about 37000-49000, because molecular weight can not must be hydrolyzed into it amino acid and polypeptide earlier and just be absorbed by body directly by body and skin absorption greatly.Amino acid and polypeptide have various physiological functions to human body, available it treat various diseases, for example: aspartic acid can be treated hepatic diseases, and its sylvite and magnesium salts have step-down and diuretic properties; The function temper official that L-glutamic acid is used for gynaecology is hemorrhage; Arginine can be treated male infertility; Methionin, vitamins C salt are a kind of appetite promotors, and Histidine has antiulcer agent and promotes the cell regeneration effect, and Gelucystine can be treated alopecia and can be prevented early ageing, and halfcystine has detoxification, can prevent hepatic necrosis and anti-radiotherapy damage; Leucine can promote growth, promote gastric secretion; Tyrosine can be participated in intravital thyroxine, adrenergic synthetic.

Peptide with physiological action has the enkephalin of analgesic activity as the thymosin of: the tethelin of treatment nanism, anti-ageing and compensation immunodeficiency.The normal method of acid or alkali that adopts is come the hydrolysis albumin in existing technology, amino acid is by all or part of destruction after tending to take place hydrolysis, for example cause the partial destruction of Serine, tyrosine, Threonine often, and tryptophane destroys all with the acid hydrolysis albumin; When with basic hydrolysis, can cause the part and whole destruction of arginine and Gelucystine, influence amino acid whose utilization.All can increase the difficulty of separation and purification work and cause environmental pollution with acid hydrolysis or basic hydrolysis in addition.

The amino acid whose percentage composition amino acid of table 1 ovalbumin and seralbumin ovalbumin seralbumin arginine 5.9 6.0 histidines 2.6 4.8 lysines 6.5 12.7 tyrosine 3.7 5.5 tryptophans 1.2 1.0 phenylalanines 7.7 7.0 cystines 2.8 6.5 methionine 5.3 1.3 threonines 4.0 7.1 serines 8.2 7.0 leucines 9.9 12.0 isoleucines 7.0 3.0 valines 8.8 6.0 glutamic acid 16.5 17.0 asparatates 9.3 10.3 glycine 3.1 2.0 alanine 6.7 6.2 proline 3 .8 5.1 hydroxyprolines 0.0 0.0 nitrogen 15.76 16.0 sulphur 1.83 1.8

The present invention seeks to seek a kind of natural active matter, make original molecular weight bigger, be difficult for the Protalbinic acid that directly absorbed by human body, be hydrolyzed into a kind of peptide and amino acid whose Protalbinic acid solution that easily is absorbed by the body that contains, and production cost is low, have no side effect, and extensively is used as the raw material of nutritive medium, makeup.

The present invention is raw material with the Protalbinic acid, use papoid, the mixing that stirs of distilled water and cysteine hydrochloride, hydrolysising reacting temperature is 30-40 ℃ of PH3-8, reaction times 4-8 hour, after enzymolysis is finished, the Protalbinic acid residue that adds the heat extraction hydrolysis, the disinfection of gained solution, this its amino acid of enzyme-hydrolysis albumen solution is: (mg/ml) Methionin 0.43-6.03mg glycine 0.29-3.98mg Histidine 0.36-1.45mg L-Ala 0.86-3.18mg arginine 4.80-5.89mg Gelucystine 0.17-3.17mg ornithine 0.00-0.53mg Xie Ansuan 1.23-8.03mg aspartic acid 1.03-1.14mg methionine(Met) 0.00-8.24mg Threonine 0.68-2.93mg Isoleucine 1.07-2.68mg Serine 1.17-6.00mg leucine 0.34-8.08mg L-glutamic acid 1.41-4.53mg tyrosine 1.44-3.06mg proline(Pro) 0.00-2.58mg phenylalanine 2.65-9.22mg amino acid total content is 65.90 to 766mg/ml, and molecular weight is about 6 * 10 ⁴Polypeptide, little peptide and aminoacid mixture form, solution is white in color or is faint yellow: PH4.5-5.5, nitrogen content 〉=0.48%, mercury≤0.05mg/kg, arsenic≤0.5mg/kg, lead≤1.0mg/kg.

The present invention finishes through the following steps:

For achieving the above object, in one embodiment we with Protalbinic acid as raw material (food grade), with papoid (pharmaceutical grade, Hainan seven refers to mountain range papain factory) as lytic enzyme, add distilled water, cysteine hydrochloride, 30-45 ℃ of in temperature of finishing the back abundant mixing of stirring is, PH3-8 carries out enzymolysis, enzymolysis time 4-8 hour, the stopped reaction hydrolysis finished, and heated to boiling again, the centrifugal not Protalbinic acid dregs slag of enzymolysis of removing, get supernatant liquor, measure nitrogen content, add 7/1000ths sodium bisulfite (A.R. Shanghai reagent four XKB-001-0804-148 of factory II) packing again, amino acid content asparatate 1.14mg threonine 0.68mg serine 1.17mg glutamic acid 1.14mg glycine 0.29mg alanine 0.86mg cystine 3.17mg valine 1.23mg isoleucine 2.68mg leucine 0.34mg tyrosine 1.44mg phenylalanine 2.65mg histidine 0.36mg lysine 0.43mg arginine 4.80mg total amino acid content 65.90mg/ml protein content 7.94mg/ml in the moist heat sterilization sterilization Protalbinic acid enzymolysis solution. table 2 ovalbumin enzyme Jie solution

The material that adopts papain enzymically hydrolyse Protalbinic acid to produce is stable, and contained aminase, peptide are not destroyed.Albumin hydrolyzate has the octapeptide material, also has a big peptide in addition, and its molecular weight is about 6 * 10 ⁴Peptide and aminoacid mixture are all good than simple amino acid in intravital specific absorption of machine and speed, under normal circumstances, after simple amino acid is absorbed, in body, also to be reassembled into peptide and protein as required, and can saving a part, peptide and aminoacid mixture reconfigure process, therefore their biological value is higher than pure amino acid, and its aminoacids content (mg/ml) is as shown in table 2 after measured.

The not only stable but also natural balance of Protalbinic acid enzymolysis solution composition composition.Its physical and chemical standards is as follows:

Index request

Appearance white or yellow solution

The little acid of the sense of taste, bitter taste slightly

Nitrogen content % nitrogen content 〉=0.48

PH 5.0±0.5

Total plate count (individual/ml)≤100

Coliform (individual/ml)≤3

Pathogenic bacterium (meaning Salmonellas) do not have

Mercury (mg/kg)≤0.05

Arsenic (mg/kg)≤0.5

Plumbous (mg/kg)≤1.0

Protalbinic acid is behind the papoid enzymically hydrolyse, generate amino acid and peptide material and show that through the mouse feeding experiment mouse hair color is smooth, weight increase is very fast, according to the institute for drug control, Shanghai the medicine toxicity test is required Protalbinic acid enzymolysis solution is carried out acute toxicity and the LD50 test is as follows:

With Kunming hybrid small white mouse body weight 20g male and female half and half, every group 10, being divided into is two groups of experimental group, is same dosage group, this experimental observation seven days, with maximum tolerated dose gastric infusion 1ml/ only, be equivalent to 50ml/kg, irritate the stomach secondary every day, continuous three days, irritate back projects such as hair color, active state when observing small white mouse, find that its active state is fabulous, hair color is smooth, shinny, honey stomach.

The present invention adopts papoid to carry out the enzymolysis of Protalbinic acid.Its enzymatic hydrolysis condition gentleness is convenient to behind the enzymolysis handle, and the component of enzymolysis is stable, is convenient to control; Papoid is inexpensive, and production cost is lower.

Papoid itself is to be extensive use of as foodstuff additive, as: " tenderizer " that U.S. ADOPH produces is exactly to use papoid as foodstuff additive, and papoid can also be as medicinal, and papoid is fool proof, its ADI does not need special stipulation, the LD of papoid ₅₀Be 12.5g/kg, belong to nontoxic level.

Illustrate in greater detail the present invention with embodiment below, being interpreted as embodiment, in office where face does not limit the scope of the invention.

Embodiment:

Take by weighing Protalbinic acid 10kg (Protalbinic acid is by taking out in new freshly-slaughtered poultry, the duck's egg), 10 gram papoid (pharmaceutical grade, Hainan seven finger mountain range papain factories) add people 12kg distilled water, add cysteine hydrochloride 1 gram again, after stirring fully mixes evenly, in temperature is 40 ℃ ± 1 ℃, PH7 ± 1 reaction 8 hours, stopped reaction, hydrolysis finishes, heat to boiling, the centrifugal not Protalbinic acid dregs slag of enzymolysis of removing, supernatant liquor is surveyed nitrogen content 1.27mg/ml, adds sodium bisulfite thousand/0. 7 back packing again, the autoclaving detoxicating gets enzyme-hydrolysis albumen solution.

Aminoacids content is as follows after measured:

It is centrifugal to analyze pre-treatment: 0.4ml+0.4ml 6% sulphosalicylic acid

Sample concentration: 100 times of supernatant liquor dilutions

Consumption during sample analysis: 0.05 milliliter

The amino acid total content is 76mg/ml in measurement result (milligram quantities/every milliliter) (the amino acid whose content of each when following data are sample analysis in the consumption) amino acid content (mg/ml) amino acid content (mg/ml) lysine 6.03 glycine 3.98 histidines 1.45 alanine 3.18 arginine 5.89 cystines 0.17 ornithine 0.53 valine 8.03 aspartic acids 1.03 methionines 8.24 threonines 2.93 isoleucines 1.07 serines 6.00 leucines 8.08 glutamic acid 4.53 tyrosine 3.06 proline 2.58 phenylalanines 9.22 tryptophans 0.17 this hydrolyzate.

Claims (4)

1, a kind of enzyme-hydrolysis albumen solution, it is characterized in that with the Protalbinic acid being raw material, with the mixing that stirs of papoid, distilled water and cysteine hydrochloride, carry out enzymically hydrolyse, this enzyme-hydrolysis albumen solution obtains by following steps: hydrolysising reacting temperature is 30-40 ℃ of PH3-8, after a reaction times 4-8 hour enzymolysis is finished, the Protalbinic acid residue that adds the heat extraction hydrolysis, the disinfection of gained solution, this enzyme-hydrolysis albumen solution is amino acid contained to be: (mg/ml)

Methionin 0.43-6.03mg glycine 0.29-3.98mg

Histidine 0.36-1.45mg L-Ala 0.86-3.18mg

Arginine 4.80-5.89mg Gelucystine 0.17-3.17mg

Ornithine 0.00-0.53mg Xie Ansuan 1.23-8.03mg

Aspartic acid 1.03-1.14mg methionine(Met) 0.00-8.24mg

Threonine 0.68-2.93mg Isoleucine 1.07-2.68mg

Serine 1.17-6.00mg leucine 0.34-8.08mg

L-glutamic acid 1.41-4.53mg tyrosine 1.44-3.06mg

Proline(Pro) 0.00-2.58mg phenylalanine 2.65-9.22mg

The amino acid total content is 65.90 to 76mg/ml, and molecular weight is about 6 * 10 ⁴Polypeptide, little peptide, aminoacid mixture form, solution is white in color or is faint yellow, PH4.5-5.5, nitrogen content 〉=0.48%, mercury≤0.05mg/kg, arsenic≤0.5mg/kg, lead≤1.0mg/kg.

2, according to right 1 described enzyme-hydrolysis albumen solution, it is characterized in that carrying out the enzymically hydrolyse Protalbinic acid with papoid.

3, a kind of production method of enzyme-hydrolysis albumen solution is characterized in that it comprises:

(1) with the Protalbinic acid be raw material, adding papoid, distilled water and cysteine hydrochloride also mixes;

(2) under temperature was 30-45 ℃, the condition of PH3-8, hydrolysis reaction was 4-8 hour;

(3) after enzymolysis is finished, add the Protalbinic acid residue of heat extraction hydrolysis;

(4) to the Protalbinic acid solution behind institute's enzymically hydrolyse, disinfection.

4, a kind of purposes of enzyme-hydrolysis albumen solution is characterized in that as nourishing health care liquid or cosmetic material.