CN104672000A - Manufacturing method of ganoderma lucidum cultivation material - Google Patents
Manufacturing method of ganoderma lucidum cultivation material Download PDFInfo
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- CN104672000A CN104672000A CN201510051098.8A CN201510051098A CN104672000A CN 104672000 A CN104672000 A CN 104672000A CN 201510051098 A CN201510051098 A CN 201510051098A CN 104672000 A CN104672000 A CN 104672000A
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- turning
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- ganoderma lucidum
- fermentation
- lentinus edodes
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- 239000000463 material Substances 0.000 title claims abstract description 39
- 240000008397 Ganoderma lucidum Species 0.000 title claims abstract description 13
- 235000001637 Ganoderma lucidum Nutrition 0.000 title claims abstract description 13
- 238000004519 manufacturing process Methods 0.000 title abstract description 5
- 241000894006 Bacteria Species 0.000 claims abstract description 27
- 238000000855 fermentation Methods 0.000 claims abstract description 15
- 230000004151 fermentation Effects 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 12
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 31
- 239000002023 wood Substances 0.000 claims description 12
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 10
- 241000196324 Embryophyta Species 0.000 claims description 10
- 244000068988 Glycine max Species 0.000 claims description 10
- 235000010469 Glycine max Nutrition 0.000 claims description 10
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 10
- 229910052602 gypsum Inorganic materials 0.000 claims description 10
- 239000010440 gypsum Substances 0.000 claims description 10
- 239000004571 lime Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 239000002245 particle Substances 0.000 claims description 8
- 244000251953 Agaricus brunnescens Species 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 6
- 239000002893 slag Substances 0.000 claims description 6
- 239000004743 Polypropylene Substances 0.000 claims description 5
- 235000003434 Sesamum indicum Nutrition 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 239000004033 plastic Substances 0.000 claims description 5
- 229920003023 plastic Polymers 0.000 claims description 5
- -1 polypropylene Polymers 0.000 claims description 5
- 229920001155 polypropylene Polymers 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 4
- 230000003203 everyday effect Effects 0.000 claims description 4
- 239000011148 porous material Substances 0.000 claims description 4
- 230000005855 radiation Effects 0.000 claims description 4
- 230000002269 spontaneous effect Effects 0.000 claims description 4
- 239000010902 straw Substances 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 2
- 244000000231 Sesamum indicum Species 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 150000001413 amino acids Chemical class 0.000 abstract description 2
- 229910052799 carbon Inorganic materials 0.000 abstract description 2
- 238000003421 catalytic decomposition reaction Methods 0.000 abstract description 2
- 239000003245 coal Substances 0.000 abstract description 2
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 239000005556 hormone Substances 0.000 abstract description 2
- 229940088597 hormone Drugs 0.000 abstract description 2
- 239000002207 metabolite Substances 0.000 abstract description 2
- 102000039446 nucleic acids Human genes 0.000 abstract description 2
- 108020004707 nucleic acids Proteins 0.000 abstract description 2
- 150000007523 nucleic acids Chemical class 0.000 abstract description 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 240000000599 Lentinula edodes Species 0.000 abstract 4
- 235000001715 Lentinula edodes Nutrition 0.000 abstract 4
- 230000000975 bioactive effect Effects 0.000 abstract 1
- 235000014633 carbohydrates Nutrition 0.000 abstract 1
- 150000001720 carbohydrates Chemical class 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 abstract 1
- 208000015181 infectious disease Diseases 0.000 abstract 1
- 230000002458 infectious effect Effects 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 241000222336 Ganoderma Species 0.000 description 8
- 241000207961 Sesamum Species 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 239000000356 contaminant Substances 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 229940088623 biologically active substance Drugs 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention relates to a manufacturing method of a ganoderma lucidum cultivation material, which is a technique applying lentinus edodes stem bases to ganoderma lucidum cultivation. Compared with the prior art, the manufacturing method has the advantages as follows: 1, lentinus edodes stem base leachate is a pure natural nutrient solution and contains carbohydrates, amino acid, peptides, nucleic acid, microelements and a rich variety of biological hormones, lentinus edodes stem residues also contain a great amount of bioactive substances which are not leached out, and a lentinus edodes stem base soaking material and a cultivation raw material are mixed and piled for fermentation so as to promote the reproduction of beneficial microbes, improve microbial communities in the materials and quicken the catalytic decomposition of the cultivation material; 2, the fermented cultivation material contains high-activity beneficial bacteria and active metabolites thereof, and has a promoting effect on the growth of ganoderma lucidum mycelia, which is embodied in vigorous growth of mycelia and high infectious microbe resistance; and 3, resources such as labor and coal are saved, environmental pollution is reduced, and the method conforms to a low-carbon environmental protection concept.
Description
Technical field
The making method of a kind of ganoderma lucidum cultivation material of the present invention, is the technology be applied to by mushroom tang in cultivation of glossy ganoderma, belongs to fungus growing technique field.
Background technology
Glossy ganoderma, also known as Ganoderma Lucidum, Ganoderma, Ganoderma lucidum seu Japonicum, is a kind of rare Chinese medicine in China's medical treasure-house, is collection health care and the medicinal edible mushrooms for the whole body.
The traditional cultivating method of glossy ganoderma is grog cultivation, and planting material carries out inoculation culture after high pressure or normal-pressure sterilization, and now in material, the macromolecule organic such as Mierocrystalline cellulose has been degraded, miscellaneous bacteria in material is also killed, therefore, when planting material sends out bacterium, mycelial growth is fast, and living contaminants is few.But along with the significantly rise of the price of labour power and fuel price, grog cultivation cost increases severely, and high-temperature sterilization also causes the loss of planting material nutritive substance, causes biological transformation ratio to reduce simultaneously.
Mushroom is famous and precious edible one of the medicinal fungus of holding concurrently in the world, and current China mushroom production accounts for 70% of world's mushroom ultimate production, has the equal mushroom culture in the province of more than 70% in China.Because champignon stems stiffness of foot in children, content of cellulose are higher, palatability is poor, and therefore, mushroom is being eaten raw or in the course of processing, the tang of mushroom can remove as tankage by the producer, causes the mushroom tang accounting for mushroom weight about 30% to become waste.
Summary of the invention
For the deficiencies in the prior art, the present invention adds the immersion of mushroom tang and expects to mix and stir to carry out banking up with planting material to ferment, and do not add sterilant, sterilant during windrow, the planting material after fermentation directly packs inoculation, bacterium bag living contaminants is few, and the glossy ganoderma quality of producing is good, output is high.
The technical solution adopted in the present invention is:
A making method for ganoderma lucidum cultivation material, comprises batching, fermentation, turning, pack, inoculation, sends out bacterium, concrete operation step:
(1) prepare burden: wood chip 72-80%, soya-bean cake 16-20%, mushroom tang 3-4%, lime 1.5%, gypsum 1%, be all the quality of dry-matter, proportioning sum is 100%; Described wood chip is the deciduous tree weed tree sawdust of particle diameter 3mm-5mm; Described mushroom tang is the tankage in the Lentnus edodes course of processing, is ground into the particle of 2mm-3mm after drying;
(2) ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leach liquor is admixed in wood chip, soya-bean cake compound uniformly together with champignon stems slag, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m
3, finally cover straw mat by its spontaneous fermentation;
(3) turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3-4 time, time remaining 5d-6d, when stopping fermentation, the water content of planting material is 63%-65%;
(4) pack, inoculate, send out bacterium: the planting material fermented is after heat radiation, load the polypropylene plastics pocket of 17cm × 35cm × 0.004cm, stamp and plant bacterium hole, during pack, bag two ends and plant in bacterium hole and put into bacterial classification, then cultivating bag is emitted in dark bacteria room, temperature controls at 20 DEG C-22 DEG C, humid control at 60%-75%, through the cultivation of 26-28d, mycelia covers with pocket, enters out sesame management routinely.
Mushroom tang is applied to the method for cultivation of glossy ganoderma by the present invention, and apart from outside explanation, other operation steps is all undertaken by this area routine techniques.
The present invention's advantage is compared with prior art:
1. mushroom tang leach liquor is full-natural nutritive liquid, containing glucide, amino acid, peptide class, nucleic acid and trace element, bio-hormone also containing abundant species, also the biologically active substance do not leached in a large number is had in champignon stems slag, add and expect to mix and stir to carry out banking up with culturing raw material to ferment with the immersion of mushroom tang, promote beneficial microorganism breeding, improve the microorganism species in material, accelerate the catalytic decomposition of planting material;
2. the planting material after fermentation has high reactivity probiotics and active metabolite thereof, has accelerating effect, show as prosperous, the anti-miscellaneous bacteria ability of mycelium growth vigor strong to the growth of Ganoderma lucidum mycelium;
3. save the resource such as manpower, coal, decrease environmental pollution, meet low-carbon environment-friendly theory.
Embodiment
Below in conjunction with embodiment, the invention will be further described, and what do not illustrate in detail in embodiment is all state of the art.
Embodiment 1: a kind of making method of ganoderma lucidum cultivation material, comprises batching, fermentation, turning, pack, inoculation, sends out bacterium, concrete operation step:
1. prepare burden: wood chip 76%, soya-bean cake 18%, mushroom tang 3.5%, lime 1.5%, gypsum 1%, be all the quality of dry-matter, proportioning sum is 100%;
2. ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leach liquor is admixed in wood chip, soya-bean cake compound uniformly together with champignon stems slag, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m
3, finally cover straw mat by its spontaneous fermentation;
3. turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3 times, time remaining 5d, when stopping fermentation, the water content of planting material is 63%-65%;
4. pack, inoculate, send out bacterium: the planting material fermented is after heat radiation, load the polypropylene plastics pocket of 17cm × 35cm × 0.004cm, stamp and plant bacterium hole, during pack, bag two ends and plant in bacterium hole and put into bacterial classification, then cultivating bag is emitted in dark bacteria room, temperature controls at 20 DEG C-22 DEG C, humid control at 60%-75%, through the cultivation of 27d, mycelia covers with pocket, enters out sesame management routinely.
Embodiment 2: a kind of making method of ganoderma lucidum cultivation material, comprises batching, fermentation, turning, pack, inoculation, sends out bacterium, concrete operation step:
1. prepare burden: wood chip 76%, soya-bean cake 18%, mushroom tang 3.5%, lime 1.5%, gypsum 1%, be all the quality of dry-matter, proportioning sum is 100%;
2. ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then the leach liquor after removing slag is admixed in wood chip, soya-bean cake compound uniformly, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m
3, finally cover straw mat by its spontaneous fermentation;
3. turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3 times, time remaining 5d, when stopping fermentation, the water content of planting material is 63%-65%;
4. pack, inoculate, send out bacterium: the planting material fermented is after heat radiation, load the polypropylene plastics pocket of 17cm × 35cm × 0.004cm, stamp and plant bacterium hole, during pack, bag two ends and plant in bacterium hole and put into bacterial classification, then cultivating bag is emitted in dark bacteria room, temperature controls at 20 DEG C-22 DEG C, humid control at 60%-75%, through the cultivation of 28d, mycelia covers with pocket, enters out sesame management routinely.
Control group: glossy ganoderma grog is cultivated, concrete operation step:
1. prepare burden: wood chip 80%, soya-bean cake 18%, lime 1%, gypsum 1%, be all the quality of dry-matter, proportioning sum is 100%;
2. spice: first wood chip, soya-bean cake, lime, gypsum siccative are mixed thoroughly, then adding water stirs, makes the water content of planting material remain on 63%-65%;
3. pack, sterilizing: employing specification is the high-pressure polypropylene plastics bag charging of 17cm × 35cm × 0.005cm, carries out normal-pressure sterilization after pack, when kettle temperature reaches 100 DEG C, keeps 10h-12h;
4. cool, inoculate: naturally cool to less than 25 DEG C and access bacterial classification, then cultivating bag is emitted in dark bacteria room, temperature controls at 20 DEG C-22 DEG C, and humid control is at 60%-75%, and through the cultivation of 30d, mycelia covers with pocket, enters out sesame management routinely.
Table one glossy ganoderma biological efficiency compares
As can be seen from Table I: the ferment effect utilizing mushroom tang leach liquor to add than single leach liquor together with the ferment effect that champignon stems slag adds is good, mycelial growth rate is fast, and bacterium bag pollution rate is low, and biological efficiency is high; Compared with control group, embodiment 1 all has obvious advantage in mycelial growth rate, anti-living contaminants and output.
Claims (1)
1. a making method for ganoderma lucidum cultivation material, comprises batching, fermentation, turning, pack, inoculation, sends out bacterium, concrete operation step:
(1) prepare burden: wood chip 72-80%, soya-bean cake 16-20%, mushroom tang 3-4%, lime 1.5%, gypsum 1%, be all the quality of dry-matter, proportioning sum is 100%; Described wood chip is the deciduous tree weed tree sawdust of particle diameter 3mm-5mm; Described mushroom tang is the tankage in the Lentnus edodes course of processing, is ground into the particle of 2mm-3mm after drying;
(2) ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leach liquor is admixed in wood chip, soya-bean cake compound uniformly together with champignon stems slag, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m
3, finally cover straw mat by its spontaneous fermentation;
(3) turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3-4 time, time remaining 5d-6d, when stopping fermentation, the water content of planting material is 63%-65%;
(4) pack, inoculate, send out bacterium: the planting material fermented is after heat radiation, load the polypropylene plastics pocket of 17cm × 35cm × 0.004cm, stamp and plant bacterium hole, during pack, bag two ends and plant in bacterium hole and put into bacterial classification, then cultivating bag is emitted in dark bacteria room, temperature controls at 20 DEG C-22 DEG C, humid control at 60%-75%, through the cultivation of 26-28d, mycelia covers with pocket, enters out sesame management routinely.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510051098.8A CN104672000A (en) | 2015-02-02 | 2015-02-02 | Manufacturing method of ganoderma lucidum cultivation material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510051098.8A CN104672000A (en) | 2015-02-02 | 2015-02-02 | Manufacturing method of ganoderma lucidum cultivation material |
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| Publication Number | Publication Date |
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| CN104672000A true CN104672000A (en) | 2015-06-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510051098.8A Pending CN104672000A (en) | 2015-02-02 | 2015-02-02 | Manufacturing method of ganoderma lucidum cultivation material |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105272625A (en) * | 2015-10-31 | 2016-01-27 | 芜湖野树林生物科技有限公司 | Preparation method of culture medium capable of improving content of amino acids of lucid ganoderma |
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| KR100808942B1 (en) * | 2006-10-18 | 2008-03-04 | 최경환 | Medium composition for cultivation of oyster mushroom |
| CN103420731A (en) * | 2013-08-15 | 2013-12-04 | 邬金飞 | Compatibility of ganoderma lucidum cultivation material and preparation method of ganoderma lucidum cultivation material |
| CN103524180A (en) * | 2013-10-22 | 2014-01-22 | 邬方成 | Method for preparing ganoderma lucidum cultivation material by using production waste of pecan |
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| CN103694041A (en) * | 2013-12-05 | 2014-04-02 | 何庚挺 | Ganoderma lucidum culture medium and culture method thereof |
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2015
- 2015-02-02 CN CN201510051098.8A patent/CN104672000A/en active Pending
Patent Citations (5)
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| KR100808942B1 (en) * | 2006-10-18 | 2008-03-04 | 최경환 | Medium composition for cultivation of oyster mushroom |
| CN103420731A (en) * | 2013-08-15 | 2013-12-04 | 邬金飞 | Compatibility of ganoderma lucidum cultivation material and preparation method of ganoderma lucidum cultivation material |
| CN103524180A (en) * | 2013-10-22 | 2014-01-22 | 邬方成 | Method for preparing ganoderma lucidum cultivation material by using production waste of pecan |
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