CN104620996A - Probiotic fermentation bed for breeding livestock and poultry - Google Patents

Abstract

The invention provides a probiotic fermentation bed for livestock and poultry breeding, which is made into a fermentation bed for breeding after microbial fermentation of organic bedding materials such as Chinese herbal medicines, and livestock and poultry are placed on it for breeding, which can solve the problem of Problems such as mold, pathogenic bacteria, and parasites; can quickly and effectively degrade and digest feces and urine excreta of livestock and poultry, and do not need to clean and rinse the breeding site every day, so that no waste is discharged from the breeding site, achieving the goal of zero discharge .

Description

A probiotic fermentation bed for livestock and poultry breeding

technical field

The invention relates to the technical field of livestock and poultry breeding, in particular to a probiotic fermentation bed for livestock and poultry breeding.

Background technique

In recent years, with the development of scale and commercialization of the breeding industry, producers have been committed to improving the performance and quantity of animal production, but they have not attracted enough attention to the issues of animal food safety and environmental pollution. As a result, food safety and environmental pollution incidents continue to occur. Although it has been paid much attention by the state, it has not been effectively improved under the drive of interest factors. Therefore, more and more people are eager for the country to strengthen the monitoring of food safety and environmental pollution, so as to ensure that people can eat safe and healthy food. At present, the country has formulated a series of laws and regulations, but this cannot be effective. deterrent effect.

At present, the problems of environmental pollution and residues of livestock products caused by intensive farming in our country are relatively serious. The main reason is that most of the trace elements supplemented in the feed are in the form of inorganic salts. Because the animal body has poor digestion and absorption capacity for inorganic mineral elements and low utilization rate, it must be added in excess to play its due role. This is one, and the second is that high copper (copper sulfate), high zinc (zinc oxide) and high antibiotic diets are commonly used in pig production to improve animal performance. While causing data waste and environmental pollution, it also affects the quality of animal products and human health.

Barber et al. (1995) found for the first time that adding 10 times the normal amount of copper to animal feed can significantly improve its growth rate and feed conversion. It has also been found that high doses of zinc oxide can increase the daily feed intake and daily weight gain of piglets, reduce feed-to-meat ratio and piglet diarrhea rate. Feed manufacturers and farmers generally add 2000-3000mg/kg or even higher to suckling pig feed Zinc oxide high-dose trace elements (high copper and high zinc) as an effective growth-promoting additive has become a relatively extensive means of nutritional regulation in the pig industry.

In pig production, high copper is widely used as a growth-promoting additive. Due to the antagonism between trace elements, it also increases the amount of trace elements such as zinc and iron. Pig feed uses a large amount of copper (Cu), zinc (Zn), iron (Fe) and other inorganic trace elements due to the low absorption and utilization rate, and the trace elements are excreted in large quantities through feces, causing serious pollution to the environment (soil, water system, etc.). In view of the harm caused by the addition of high-dose trace elements, the European Community has proposed a hygienic standard for the allowable amount of copper in feed. It is stipulated that 175mg/kg for piglets within 16 weeks, and 35mg/kg for more than 17 weeks. The Canadian and American Feed Associations have set the maximum limits of copper and zinc in the diet as 125mg/kg and 500mg/kg respectively. my country has also adjusted the amount of trace elements used in feed, 200mg/kg for piglets and 35mg/kg for fattening pigs. High copper can not only cause copper poisoning in pigs, but also cause the lack of certain nutrients (such as VA, VD, VE, VB2) in pigs. When the copper content in the feed is as high as 250ppm, it will have a bad impact on the quality of pork, which will lead to food safety. question. In consideration of environmental protection, the Netherlands no longer allows the use of high copper and high zinc as growth promoters in diets: Japan also imposes strict restrictions on the amount of copper and zinc used.

Trace elements such as copper and zinc account for the vast majority of environmental pollution caused by modern animal husbandry production. This not only leads to excessive nutrition and economic waste of animal trace elements, but also causes serious pollution to the ecological environment. According to reports, the annual use of trace element additives in my country is 150,000 to 180,000 tons, but due to its low utilization rate, about 100,000 tons of minerals that are not used by animals are excreted with feces and urine to pollute the environment and become a major public hazard. The addition of inorganic trace elements such as copper in general feed is 20 to 40 times the normal requirement of the body. 90% of copper in feed is excreted in feces after metabolism. Copper is a non-degradable substance, which will inevitably cause serious pollution of soil, water and vegetation. hinder the development of environmentally friendly agriculture. Most of the zinc comes from unabsorbed exogenous zinc in the diet. Studies have shown that the daily zinc excretion of pigs fed the high-dose zinc diet group is 33 times that of the basal diet group. Such a high dose of zinc passes through the feces Excreted into the environment, the accumulation of soil zinc and pollution of the environment. According to relevant experts in Japan, if all the zinc in pig manure stays in the soil, the zinc content will exceed the standard of 120mg/kg within 17 years. The polluted land will no longer be arable, and zinc is more toxic to fish and aquatic organisms Fishery water quality requires zinc to be within 0.1mg/L. Excessive zinc will pollute groundwater after being excreted by livestock and hinder the sustainable development of animal husbandry.

Such a high dose of trace elements will inevitably affect the quality of livestock products. my country's health standard for copper limits in meat products (GB 15199-94) stipulates that the copper content in pork should not exceed 10ppm. A high-copper diet can increase the copper content of pig liver by 10-15 times. Human consumption of high-copper-containing livestock products can cause copper to accumulate in tissues such as liver, brain, and kidney, increase the level of free radicals in the body, and change lipids. Class metabolism, leading to atherosclerosis and accelerating cell aging and death.

Since the 1950s, animal scientists discovered that low-concentration antibiotics in feed can significantly promote the growth of livestock and poultry, and feed antibiotics have been widely used. Almost all feed-producing countries in the world add various antibiotics to feed. It should be said that for nearly half a century, feed antibiotics have indeed played a positive role in preventing animal diseases, promoting animal growth, and improving feed remuneration. According to statistics, there are more than 20 kinds of antibiotics and more than 10 kinds of synthetic antibacterial drugs used in feed in the world. The period from the 1960s to the early 1980s was the period when antibiotics were most widely used in the feed industry.

As we all know, antibiotics have made great contributions to the development of animal husbandry as production accelerators. However, problems such as drug residues and drug resistance caused by the non-standard use of antibiotics have attracted great attention. Over the years, due to the long-term and extensive use of antibiotics by Chinese farmers, the beneficial bacteria of livestock and poultry have been killed, resulting in flora imbalance, double infection, drug resistance, etc., and the immune function, disease resistance and production performance of livestock and poultry have declined. The cost of farming has increased. Not only that, in the past many years, experts in Europe and the United States have conducted investigations on this, and concluded that the resistance of bacteria to drugs in animals may be transferred to humans. Drug resistance can even be transmitted to the human body through livestock and poultry products, posing a threat to human health and treatment. According to a report in the Chinese Veterinary Journal (1992), the frightening "mad cow disease" in recent years is also caused by a three-in-one injection made from long-term injections of antibiotics, hormones, and sheep and horse offal. The occurrence of "antibiotic enteritis" is also closely related to the abuse of antibiotics.

The use of antibiotics not only inhibits or kills pathogenic microorganisms, but also inhibits or kills beneficial bacteria in animals, breaks the micro-ecological balance, causes animal vitamin deficiency, reduces immunity, and even makes non-sensitive flora and The fungus multiplies in large numbers, causing digestion and absorption disorders and secondary infections, resulting in disease or death of animals. For example, long-term use of antibiotics will make the livestock and poultry organisms dependent, limit the function of immune cells in the body, reduce the immune function of the organism, and reduce the ability to resist diseases. Antibiotics enter the animal's blood circulation after they play a role in the animal's digestive tract along with the feed, and most of them are excreted with urine after being filtered by the kidneys, but some remain in the animal's body. These residual antibiotics accumulate in animal products such as meat, eggs, milk, and hair, directly threatening human health. The use of antibiotics may cause genetic mutations, deformities, and carcinogenicity in animals; antibiotics are excreted in large quantities from animals, and may also cause environmental pollution. According to relevant reports, long-term use of low-dose antibiotics in feed will increase the chance of drug resistance.

In view of the serious side effects of antibiotics, the European Commission legislated to prohibit the addition of bacitracin, spiramycin, virginiamycin, and tylosin to feed 4 antibiotics from July 1, 1999. It also stipulates that drug residues must not be detected in animal tissues, which puts forward higher requirements for my country's export of livestock and poultry products. Currently, the US FDA has removed penicillin, tetracycline, erythromycin, lincomycin, tylosin, and virginiamycin from agricultural use.

In order to solve the hidden dangers of animal products, scholars from various countries have carried out research on green feed additives. There are microecological preparations and enzyme preparations. Microecological preparations can prevent diseases, promote growth, and improve feed utilization by increasing beneficial bacteria in the digestive tract, inhibiting harmful bacteria, adjusting or maintaining the balance of intestinal flora; enzymes The preparation contains a variety of enzymes that can decompose nutrients in feed, help animals digest and absorb, promote animal growth, and improve feed utilization. But these products can not fully achieve the application effect of feed antibiotics. Chinese herbal medicine is a traditional medicine for disease prevention and treatment in our country, and now people place their hopes on Chinese herbal medicine for animal health care. Most studies have shown that Chinese herbal medicine feed additives can promote the growth and health of livestock and poultry, and have unique advantages such as small toxic and side effects, no residue, and no drug resistance. , is restricted in application.

In the field of human medicine, great progress has been made in the research on the coordination between the active ingredients of traditional Chinese medicine and trace elements. Components) are fully recognized as important active ingredients in traditional Chinese medicine and their significance to life, health and curative effect. The research on trace elements has also gradually deepened from simple determination. According to the research on the coordination theory of active ingredients of traditional Chinese medicine and trace elements, it is shown that the coordination of trace elements and organic ingredients in Chinese herbal medicines has strong pharmacological activity, and some of its activities are higher than those of organic compounds. Most of the pharmacological effects are combined forms, which are easier to penetrate into tissues than inorganic salts alone. There is no domestic research report on the application of plant extracts and trace element complexes in the feed industry.

With the enhancement of global food safety awareness, under the current breeding conditions, how to maximize the production performance of animals while ensuring the health of animals, improving the body's immunity and resistance to external factors such as diseases has become more and more important. The core content of livestock and poultry production. Therefore, the company researches and applies a new type of compound livestock and poultry micro-ecological preparation fermented with probiotics combined with trace elements and Chinese herbal medicines to replace antibiotics and reduce the amount of trace elements. This product can overcome the adverse consequences of antibiotics and chemical drugs by changing the intestinal microflora, and has the functions of promoting growth, preventing and treating diseases, and health care. It can significantly improve the production performance of livestock and poultry, and improve the utilization rate of feed. This can not only strengthen the production of safe animal products and environmental protection, and protect the health of the people, but also promote the sustainable development of animal husbandry, meet the needs of my country's foreign trade, and also meet the needs of the country's political and economic stability.

In the prior art, livestock and poultry breeding produces a large amount of feces, which contain a large amount of nitrogen, phosphorus and organic pollutants, etc. After fermentation, a large amount of harmful gases such as ammonia nitrogen, hydrogen sulfide, skatole, and methane will be produced. The gas not only destroys ecology, but also directly affects human health. In addition, livestock and poultry farms (households) have poor environmental awareness, randomly pile up livestock and poultry manure and discharge sewage, and the sewage enters the water system with rainwater and sewage, seriously polluting groundwater, resulting in continuous deterioration of soil and water quality. Since the reform and opening up, my country's animal husbandry has made great progress. At present, my country's meat production ranks first in the world, and it has become a veritable animal husbandry country. However, animal husbandry is still a low-yield industry in my country. In order to improve the production performance of animals, the multi-use and abuse of feed additives (copper, zinc, antibiotics and production-promoting additives) have become a common problem in the feed industry. The country has also issued some corresponding laws and regulations to limit, but the effect is not satisfactory. Some of these additives remain in animal products and are eaten and directly affect people's health; some of them enter the environment directly with animal excrement, causing certain pollution to soil, air, and water sources, and then enter the human body through the food chain, which also potentially threatens human health. Therefore, how to solve the safe production of animal husbandry and the impact on the environment has become an urgent problem to be solved in the sustainable development of animal husbandry. With the rapid development of my country's economy and the promotion of ecological agriculture, the demand for environmentally friendly products in the feed industry is increasing.

Contents of the invention

The technical problem solved by the present invention is to provide a probiotic fermentation bed for livestock and poultry breeding, which is made into a fermentation bed for breeding after microbial fermentation of organic bedding materials such as Chinese herbal medicines, and placing livestock and poultry in Breeding on it can solve the problems of mold, pathogenic bacteria, and parasites; it can quickly and effectively degrade and digest the excrement of livestock and poultry, and there is no need to clean and rinse the breeding site every day, so that no waste is discharged from the breeding site , to achieve the goal of zero emissions.

The technical scheme adopted in the present invention is:

A kind of probiotic fermentation bed for livestock and poultry breeding, its preparation method comprises:

① Fermentation of beneficial strains: anaerobic fermentation including anaerobic microorganisms and aerobic fermentation of aerobic microorganisms; nematodes and Bacillus subtilis; the anaerobic fermentation is to inoculate the anaerobic microorganisms into the liquid medium for anaerobic constant temperature culture, and obtain the anaerobic fermentation liquid through the first-level expansion culture and the second-level expansion culture; Oxygen fermentation is to inoculate aerobic microorganisms into liquid medium for aerobic constant temperature culture, and obtain aerobic fermentation liquid through primary expansion culture and secondary expansion culture;

②Preparation of probiotic fermentation bed: Mix all kinds of raw materials for the preparation of fermentation bed materials evenly to obtain organic bedding, then inoculate the anaerobic fermentation liquid and aerobic fermentation liquid into the organic bedding for solid-state fermentation. After 3-5 days, when the temperature of the solid material reaches 50°C, evenly spread the organic bedding material into the fermentation bed, the thickness of which is not less than 60cm, and then breed the livestock and poultry; the organic bedding material includes sawdust, rice husk, Bran and the first compound Chinese herbal medicine, the first compound Chinese herbal medicine includes dandelion, Houttuynia cordata, capillary chen, qinpi, Folium chinensis, epimedium, Artemisia annua, Andrographis paniculata, Solanum solanum, Boluohui, Dijincao, Guanzhong and Tujingpi.

Preferably, the specific process of the anaerobic fermentation described in step ① is to inoculate the preserved plant lactic acid bacteria and Enterococcus faecalis into the liquid medium, and culture it at a constant temperature of 37°C for 24 hours under anaerobic conditions. When the pH of the fermentation broth is <4.5, Transplant it into a 50L fermenter for primary expansion culture, and culture it at a constant temperature of 37°C for 24 hours under anaerobic conditions. When the pH of the fermentation broth in the fermenter is <4.5, transplant it into a 1000L fermenter for Secondary expansion cultivation, under anaerobic conditions, 37°C constant temperature cultivation for 30 hours, when the pH of the fermentation liquid in the fermenter is <4.5, the fermentation is terminated to obtain the anaerobic fermentation liquid.

Preferably, the specific process of the aerobic fermentation described in step ① is to inoculate the aerobic microorganisms into the liquid medium, and cultivate them at a constant temperature of 30°C for 24 hours under aerobic conditions. Planted in a 50L fermenter for primary expansion cultivation, cultured at a constant temperature of 30°C for 24 hours under aerobic conditions, when the pH of the fermentation broth in the tank was <5.5, transplanted to a 1000L fermenter for secondary expansion cultivation, Under aerobic conditions, culture at a constant temperature of 30° C. for 30 hours, and when the pH of the fermentation liquid in the fermenter is less than 5.5, the fermentation is terminated to obtain the aerobic fermentation liquid.

Preferably, the components of the liquid medium used in the anaerobic fermentation and aerobic fermentation include: glucose, ammonium sulfate, manganese sulfate, magnesium sulfate, potassium dihydrogen phosphate, corn, soybean meal and deionized water.

Preferably, the ingredients and parts by weight of the organic litter described in step ② include 40% sawdust, 40% rice husk, 5% bran and 15% of the first compound Chinese herbal medicine, the first compound Chinese herbal medicine The parts by weight of various ingredients are: 1-2 parts of Dandelion, 1-2 parts of Houttuynia cordata, 1-2 parts of capillary, 1-2 parts of Qinpi, 1-2 parts of Daqingye, 1-2 parts of Epimedium 2 parts, 1-2 parts of Artemisia annua, 1-2 parts of Andrographis paniculata, 1-2 parts of Solanum nigrum, 2-4 parts of Boluohui, 1-2 parts of Dijincao, 1-3 parts of Guanzhong and 1 part of Solanum bark -4 parts.

Preferably, the anaerobic fermentation liquid and the aerobic fermentation liquid described in step ② are mixed evenly at a volume ratio of 1:1, and then inoculated into the organic litter at an inoculation amount of 10% (mass percentage) .

Preferably, the water content of the organic litter after inoculation does not exceed 60%.

Preferably, the size of the fermentation bed in step ② is not less than 20m ² , and the livestock and poultry are pigs, and the average area occupied by each pig is 1.0-1.5m ² .

The key technical point of the present invention is to ferment organic bedding such as Chinese herbal medicine and make it into a fermentation bed to breed livestock and poultry. The probiotic fermentation bed of the present invention makes up for the shortcomings of the existing ecological bed through the combination of Chinese herbal medicine and ecological bed. The disadvantage is to solve the problems of mold, pathogenic bacteria, and parasites; it is especially suitable for pig breeding. The present invention uses the ecological breeding method of the probiotic fermentation bed to completely solve the problem of environmental pollution caused by pig raising. Since the organic litter covered on the fermentation bed contains active beneficial microorganisms (i.e. the above-mentioned several bacterial species), it can quickly and effectively degrade and digest the excrement of pigs, and there is no need to clean the pigsty every day , Flush the pig house, so that no waste is discharged from the pig farm, and there is no sewage for flushing the pig house, which can save more than 70% of water. Really achieve the goal of zero emissions from pig farming. At the same time, the environment of the pig house is improved. The pig house with the fermentation bed is designed with large windows, fully open in summer and closed with roller shutters in winter for heat preservation. The roof of the pig house is equipped with an electric exhaust skylight, which is well ventilated and the temperature and humidity are suitable for the growth of pigs. Pig manure and urine are quickly decomposed by beneficial microorganisms, and there is no odor and no breeding of flies in the pig house. According to the ambient air test, the content of harmful gases such as carbon dioxide, hydrogen sulfide, and ammonia in the micro-ecological fermentation bed pig house is lower than that of traditional greenhouse pigs housing, which is much lower than the Ministry of Agriculture's "Environmental Quality Standards for Livestock and Poultry Farms". In addition, the first compound Chinese herbal medicine powder is used in the micro-ecological fermentation bed to further improve the fermentation bed to prevent the growth of mold, pathogenic bacteria and parasites, which greatly reduces the incidence of pigs and effectively solves the problem of pig farms. When respiratory diseases occur in pig herds, if no medicine is used, the growth rate of pigs will be slow, the survival rate and growth rate will be low, the feed remuneration will be reduced, and the benefits will outweigh the benefits.

Detailed ways

Embodiments of the present invention will be described in detail below. It should be noted that, in the case of no conflict, the embodiments in the present application and the features in the embodiments can be combined arbitrarily with each other.

Embodiment 1: the kind of beneficial bacteria and fermentation method thereof

The strains used in the fermentation bed include plant lactic acid bacteria, Enterococcus faecalis, Geotrichum candidum, Saccharomyces cerevisiae, actinomycetes and Bacillus subtilis. Plant lactic acid bacteria and Enterococcus faecalis are subjected to anaerobic fermentation, and other strains are subjected to aerobic fermentation.

Anaerobic fermentation: Inoculate well-preserved plant lactic acid bacteria and Enterococcus faecalis into liquid medium (1000mL Erlenmeyer flask volume is generally 500mL) in an anaerobic environment, that is, under anaerobic conditions at 37°C for 24 hours at a constant temperature, when fermented When the pH of the solution is less than 4.5, transplant it into a 50L fermenter for primary expansion culture, and then culture it under anaerobic (ie anaerobic) conditions at 37°C for 24 hours at a constant temperature. , and then transplanted into a 1000L fermenter for secondary expansion cultivation, and then cultured at a constant temperature of 37°C for 30 hours under anaerobic conditions. When the pH of the fermentation liquid in the fermenter was <4.5, the liquid fermentation ended and anaerobic fermented bacteria.

Aerobic fermentation: Inoculate the preserved Geotrichum candidum, Saccharomyces cerevisiae, actinomycetes and Bacillus subtilis into liquid medium (1000mL Erlenmeyer flask capacity is 500mL), in an aerobic environment, 30 ℃ constant temperature culture for 24h, when fermentation When the pH of the fermented liquid is less than 5.5, transplant it into a 50L fermenter for primary expansion cultivation, and then culture it at a constant temperature at 30°C for 24 hours under aerobic conditions. When the pH of the fermented liquid in the fermenter is less than 5.5, transplant it Planted in a 1000L fermenter for secondary expansion cultivation, under aerobic conditions, cultured at a constant temperature of 30°C for 30 hours, when the pH of the fermentation liquid in the fermenter was <5.5, the liquid fermentation ended, and the aerobic fermented bacterial liquid was obtained.

Preferably, the components of the liquid medium used in the above-mentioned anaerobic fermentation and aerobic fermentation include: glucose, ammonium sulfate, manganese sulfate, magnesium sulfate, potassium dihydrogen phosphate, corn, soybean meal and deionized water.

Embodiment 2: preparation fermentation bed

Firstly mix 40% sawdust, 40% rice husk, 5% bran, and 15% of the first compound Chinese herbal medicine by weight, which is the organic bedding, and then mix the anaerobic fermentation bacteria liquid with aerobic The fermented bacterial liquid is mixed at a volume ratio of 1:1, and the mixed bacterial liquid is inoculated into the organic litter according to the inoculation amount of 10%, and the water content is controlled at no more than 60%, and then solidified in a stacking manner. Fermentation. After 3-5 days, when the temperature of the solid material reaches 50°C, spread the fermented organic bedding with a thickness of not less than 60cm on a fermentation bed of not less than 20m2, and carry out ecological breeding of pigs, with an average density of 1.0 per pig m2～1.5m2. The first compound Chinese herbal medicine is 80-mesh powder.

The invention adopts the ecological breeding method of the fermented bed of probiotics to completely solve the problem of environmental pollution caused by raising pigs. Because the organic bedding material laid on the above-mentioned fermentation bed contains active beneficial microorganisms (that is, the above-mentioned several bacterial species), it can quickly and effectively degrade and digest the excrement of pigs, and it is not necessary to clean the pigsty every day. Flush the pig house, so that no waste is discharged from the pig farm, and there is no sewage for flushing the house, which can save more than 70% of water. Really achieve the goal of zero emissions from pig farming. At the same time, the environment of the pig house is improved. Because the pig house with the fermentation bed is designed as a large window, it is fully open in summer and closed with roller shutters in winter for heat preservation. The roof of the pig house is equipped with an electric exhaust skylight, which is well ventilated and the temperature and humidity are suitable for the growth of pigs. . Pig manure and urine are quickly decomposed by beneficial microorganisms, and there is no odor and no breeding of flies in the pig house. According to the ambient air test, the content of harmful gases such as carbon dioxide, hydrogen sulfide, and ammonia in the micro-ecological fermentation bed pig house is lower than that of traditional greenhouse pigs housing, which is much lower than the Ministry of Agriculture's "Environmental Quality Standards for Livestock and Poultry Farms". In addition, the first compound Chinese herbal medicine powder is used in the micro-ecological fermentation bed to further improve the fermentation bed to prevent the growth of mold, pathogenic bacteria and parasites, which greatly reduces the incidence of pigs and effectively solves the problem of pig farms. When respiratory diseases occur in pig herds, if no medicine is used, the growth rate of pigs will be slow, the survival rate and growth rate will be low, the feed remuneration will be reduced, and the benefits will outweigh the benefits.

In addition, it should be noted that chemical disinfection drugs and antibiotics cannot be used in the pig house with a fermentation bed. If used, it will kill and inhibit microorganisms, or inhibit their reproduction, which will reduce the activity of microorganisms; It is beneficial to prevent people from using substances such as antibiotics to raise pigs.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and changes. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims (8)

1., for a probiotics fermention bed for livestock and poultry cultivation, it is characterized in that: preparation method comprises:

(1) probiotics fermentation: comprise the anaerobic fermentation of anaerobe and the aerobic fermentation of aerobic microorganisms; Described anaerobe comprises plant lactobacillus and enterococcus faecalis, and described aerobic microorganisms comprises Geotrichum, S. cervisiae, actinomycetes and bacillus subtilis; Described anaerobic fermentation is inoculated in liquid nutrient medium by anaerobe to carry out anaerobic constant temperature culture, and obtain anaerobic fermentation bacterium liquid by one-level expansion cultivation and secondary expansion cultivation; Described aerobic fermentation is inoculated in liquid nutrient medium by aerobic microorganisms to carry out aerobic constant temperature cultivation, and obtain aerobic fermentation bacterium liquid by one-level expansion cultivation and secondary expansion cultivation;

(2) probiotics fermention bed is prepared: mixed by the various raw materials of preparation fermentation bed material, obtain organic bedding and padding, again the anaerobic fermentation bacterium liquid in step (1) and aerobic fermentation bacterium liquid are seeded in organic bedding and padding, carry out accumulation solid state fermentation, after 3-5 days when solid material temperature reaches 50 DEG C, by organic bedding and padding uniform spreading on livestock and poultry cultivation bed, spread thickness and be not less than 60cm, make described probiotics fermention bed, then cultivate placed on it for livestock and poultry; Described organic bedding and padding comprise sawdust, rice husk, wheat bran and the first compound Chinese herb, and described first compound Chinese herb comprises dandelion, cordate houttuynia, oriental wormwood, the bark of ash, folium isatidis, barrenwort, sweet wormwood, Herba Andrographitis, black nightshade, macleaya cordata, wolf's milk, rhizome of cyrtomium and Golden Larch Bark.

2. probiotics fermention bed according to claim 1, it is characterized in that: the detailed process of anaerobic fermentation described in step (1) is the plant lactobacillus that will preserve, enterococcus faecalis is inoculated in liquid nutrient medium, 37 DEG C of constant temperature culture 24h under anaerobic, as zymotic fluid pH < 4.5, cultivation is expanded by carrying out one-level in its culture transferring to the fermentation tank of 50L, 37 DEG C of constant temperature culture 24h under anaerobic, as zymotic fluid pH < 4.5 in fermentation tank, cultivation is expanded by carrying out secondary in its culture transferring to the fermentation tank of 1000L, the lower 37 DEG C of constant temperature culture 30h of oxygen free condition, as zymotic fluid pH < 4.5 in fermentation tank, terminate fermentation, obtain described anaerobic fermentation bacterium liquid.

3. probiotics fermention bed according to claim 1, it is characterized in that: the detailed process of the aerobic fermentation described in step (1) is inoculated in liquid nutrient medium by described aerobic microorganisms, the lower 30 DEG C of constant temperature culture 24h of aerobic conditions, as zymotic fluid pH < 5.5, cultivation is expanded by carrying out one-level in its culture transferring to the fermentation tank of 50L, 30 DEG C of constant temperature culture 24h under aerobic conditions, when in tank during zymotic fluid pH < 5.5, cultivation is expanded by carrying out secondary in its culture transferring to the fermentation tank of 1000L, 30 DEG C of constant temperature culture 30h under aerobic conditions, when in fermentation tank during zymotic fluid pH < 5.5, terminate fermentation, obtain described aerobic fermentation bacterium liquid.

4. probiotics fermention bed according to claim 1, is characterized in that: the composition of liquid nutrient medium used in described anaerobic fermentation and aerobic fermentation comprises: glucose, sulfate of ammoniac, manganese sulphate, magnesium sulfate, potassium dihydrogen phosphate, corn, dregs of beans and deionized water.

5. probiotics fermention bed according to claim 1, it is characterized in that: the composition of organic bedding and padding described in step (2) and parts by weight thereof: comprise the sawdust of 40%, the rice husk of 40%, the wheat bran of 5% and first compound Chinese herb of 15%, in described first compound Chinese herb, the parts by weight of various composition are: dandelion 1-2 part, cordate houttuynia 1-2 part, oriental wormwood 1-2 part, bark of ash 1-2 part, folium isatidis 1-2 part, barrenwort 1-2 part, sweet wormwood 1-2 part, Herba Andrographitis 1-2 part, black nightshade 1-2 part, macleaya cordata 2-4 part, wolf's milk 1-2 part, rhizome of cyrtomium 1-3 part and Golden Larch Bark 1-4 part.

6. probiotics fermention bed according to claim 1, it is characterized in that: the anaerobic fermentation bacterium liquid described in step (2) and aerobic fermentation bacterium liquid mix with the volume ratio of 1:1, then be seeded in organic bedding and padding by the inoculum concentration of mass percent 10%.

7. probiotics fermention bed according to claim 1, is characterized in that: in step (2), the water content of postvaccinal described organic bedding and padding is no more than 60%.

8. probiotics fermention bed according to claim 1, is characterized in that: the size of the probiotics fermention bed described in step (2) is for being not less than 20m ², described livestock and poultry are pig, and on average every pig area occupied is 1.0 ~ 1.5m ².