CN104430568B - A kind of botanical pesticide for preventing and treating matrimony vine thrips - Google Patents

Abstract

The invention relates to a botanical pesticide, in particular to a botanical pesticide for preventing and treating Lycium barbarum thrips. For the pharmaceutical industry, the compound pharmaceutical industry is added with an emulsifier and stirred to become a mother liquor. Finally, the mother liquor is dripped with water, stirred with high shear, and filtered to obtain a preparation. Any mixture of azadirachtin, crotonine, veratrine, pyrethrins, azadirachtin or Cnidium; the present invention provides a compounding range that can improve the botanical pesticide, which is beneficial to further improve the potency of the botanical pesticide. High efficiency, in order to solve the serious harm of Lycium barbarum thrips and excessive pesticide residues of Lycium barbarum.

Description

A kind of botanical pesticide for preventing and treating Lycium barbarum thrips

technical field

The invention relates to a botanical pesticide, in particular to a botanical pesticide for preventing and treating wolfberry thrips.

Background technique

At present, there are more than 7,400 species of thrips recorded in the world, divided into 2 suborders and 8 families, and more than 400 species of thrips have been recorded in China. Thrips is an important global omnivorous insect, mainly herbivorous, with a wide range of host plants, and mainly damages a variety of grains, fruits, vegetables, flowers, and horticultural plants. This kind of pests directly damage the young epidermis of plants by feeding or laying eggs with file-suction mouthparts, causing damage to plant organs and affecting photosynthesis, thereby affecting the appearance quality and yield of products, and reducing economic value; some thrips It can also spread a variety of plant viruses and cause indirect damage, making plant virus diseases popular among crops. The direct damage of thrips to the host plant is less than the indirect damage caused by the viruses it transmits. Thrips pests are small in size and often hide in the flower buds of plants. Their damage is difficult to find, and their fecundity is extremely strong. They can break out in large numbers in a short period of time. Chemical control is mainly used in agricultural production. For a long time, due to the unscientific and rational use of chemical pesticides, the pesticide residues in agricultural economic crops have exceeded the standard, which has brought great challenges to safe production.

However, botanical pesticides generally have the advantages of less impact on human and animal safety and environmental quality, which just make up for the shortcomings of chemical pesticides. However, the cost of using botanical pesticides is high, the action is slow, the duration of effect is relatively short, and the insecticidal spectrum is narrow, so there are still certain difficulties in its promotion and popularization.

In recent years, Lycium barbarum thrips has become one of the most serious pests and the most difficult to control. In the production of Lycium barbarum, the main population is Indian thrips. Simple management of thrips seriously affects and restricts the healthy development of wolfberry industry. Therefore, the blind and passive use of chemical agents by Ci Nong not only failed to achieve good control effects, but also caused the serious problem of pesticide residues exceeding the standard, which made the safety of wolfberry products decrease and the export of a large number of products was restricted.

There is currently no method for overcoming the aforementioned drawbacks.

Contents of the invention

The purpose of the present invention is to overcome the defects of the prior art, provide a kind of compounding range that can improve the botanical pesticides, help to further improve the high efficiency of the botanical pesticides, to solve the serious harm of wolfberry thrips and excessive residues of wolfberry pesticides, etc. Problem of a botanical pesticide for the control of Lycium barbarum thrips.

The present invention is realized in the following manner:

A botanical pesticide preparation for preventing and treating Lycium barbarum thrips, characterized in that: the preparation method of the pesticide preparation is firstly dissolving the original drug with a solvent to form a solution, then mixing the solution into a compound drug solution, and then adding the compound drug solution to After the emulsifier is stirred, it becomes the mother liquor, and finally the mother liquor is dripped with water, stirred by high shear, and filtered to obtain the preparation. Any mixture of pyrethrins, azadirachtin or cnidium;

The mass concentration of the former medicine is successively 15% of matrine, 6% of azalea, 1% of azadirachtin, 2% of crotonine, 5% of veratrine, 25% of pyrethrins, 5% of azadirachtin, Nicotine 95%, Fructus Cnidii 30%, the concentration of the solution to be mixed obtained after dilution of the former medicine is sequentially neem 0.01g/L, matrine 0.01g/L, nicotine 0.03g/L, pyrethrum 0.08g/L L, Fructus Cnidii Concentration 0.05g/L, Phyllostachys chinensis 3g/L, Neem 0.05g/L, Veratrine 0.35g/L, Montroitine 20g/L;

The mixed volume ratio of the solution is: nicotine:neem=1:5～5:1, nicotine:matrine=1:5～5:1, nicotine:cnidium=1:5～5: 1. Nicotine: Neem = 1:5 ~ 5:1, Nicotine: Paleopsis = 1:5 ~ 5:1;

Described formulation formula is following any one:

The volume ratio is: nicotine: neem = 1:5 ~ 5:1 + 20% solvent + 8% emulsifier, pure water to make up to 100%;

The volume ratio is: nicotine: matrine = 1:5 ~ 5:1 + 20% solvent + 8% emulsifier, pure water to make up to 100%;

The volume ratio is: nicotine: cnidium = 1:5 ~ 5:1 + 25% solvent + 8% emulsifier, pure water to make up to 100%;

The volume ratio is: nicotine: neem = 1:5 ~ 5:1 + 18% solvent + 8% emulsifier, pure water to make up to 100%;

The volume ratio is: nicotine: bitter bark = 1:5 ~ 5:1 + 35% solvent + 8% emulsifier, pure water to make up to 100%;

The above content is volume percentage;

The solvent is acetone, alcohol or water, and the emulsifier is concentrated milk 500, OP-10 or Tween 80.

The present invention has following effect:

The experimental methods used in the following examples are conventional methods unless otherwise specified.

The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

Indoor toxicity determination of embodiment 1, 9 kinds of botanical insecticide single agents to thrips

Test insects: Lycium barbarum thrips was collected from the garden farm of Yinchuan City, Ningxia, and was identified as a composite population. Continue to breed indoors, and take the second instar nymphs of Lycium barbarum Thrips for testing.

Test medicaments: 15% matrine, 6% phyllotine, 1% azadirachtin, 2% crotonine, 5% veratrine, 25% pyrethrins, 5% azadirachtin, 95% nicotine, 30% Fructus Cnidii, the source of this raw material is Baoji Fangsheng Biological Development Co., Ltd.;

Acetone is used as the solvent, and Tween-80 is used as the emulsifier, and it is made by fully stirring evenly.

Determination method: the method of soaking leaves with medicinal film is adopted, and the specific steps are as follows:

(1) Dissolve the above-mentioned 9 kinds of botanical medicines with a solvent first, and then dilute to several gradients with water-emulsifier to ensure that the prepared medicine solution is transparent and uniformly dispersed at the test concentration. After preparing the medicinal liquid, first make the insecticide film: dissolve the botanical agent with acetone, dilute it with water-emulsifier to a certain concentration, spray it evenly into the insecticulture box, and let it dry naturally indoors to form a medicinal film.

(2) Dipping leaves of Lycium barbarum with the same concentration of medicinal solution, and after the medicinal film and soaked leaves are dried, put the medicinal leaves into the insect culture box with medicinal film to form the method of soaking leaves with medicinal film. Finally, about 30 test insects were connected, cultured and observed, and the mortality rate was calculated.

The water film box and the leaves of Lycium barbarum soaked in water were used as the control group, and each concentration was treated with 4 replicates. Control group mortality rate <10% is an effective test.

Drug toxicity measurement: DPS analysis software is used for data processing, and the toxicity regression equation, LC50 value and its 95% confidence limit of each drug are obtained.

A co-toxicity factor >20 indicates synergistic effect of two single doses; ≤-20 indicates antagonistic effect, and -20~20 indicates additive effect.

Table 19 Indoor toxicity determination of botanical agents

From the data in Table 1, it can be known that the LC50 of neem, matrine and nicotine on thrips is low, and the lower the LC50, the stronger the contact toxicity, so these three agents were selected for compounding with the other six plant-derived agents. Match, and investigate the contact toxicity to thrips.

Embodiment 2, Neem and other 8 kinds of botanical agents are tested for the indoor toxicity of thrips

The botanical pesticide neem screened out in Example 1 is mixed with other 8 kinds of original drugs according to the volume ratio of 5:1, 2:1, 1:1, 1:2, 1:5 to obtain the mixed compound Medicament (concentration of each agent is LC50 value, Neem: 0.01g/L; Matrine: 0.01g/L; Nicotine: 0.03g/L; Pyrethrum: 0.08g/L; Cnidium: 0.05g/L; Pittonia: 3g/L; Neem: 0.05g/L; Veratrine: 0.35g/L; Monarchetine: 20g/L). Investigate the indoor toxicity determination of 8 kinds of mixed medicines to thrips, and use the method of soaking leaves with medicine film for determination, and the specific steps are the same as in Example 1.

Table 2 shows the combined toxicity test results of the above eight mixed agents.

Table 2 Toxicity determination of binary mixture of neem and other 8 kinds of botanical agents

It can be seen from the data in Table 2 that neem: matrine = 1:5 mixed, the co-toxicity factor is 31.08>20, which has a synergistic effect, the greater the co-toxicity factor, the more obvious the synergistic effect, indicating that its The synergistic effect is the most obvious, so the mixture of neem and matrine has a higher insecticidal activity against thrips than a single agent; neem:nicotine=1:5～2:1 mixed, the co-toxicity factors are greater than 20, showing that their mixing has synergistic effect, the insecticidal activity of neem and nicotine is higher than that of single agent against thrips; among them, neem:nicotine=1:2, the co-toxicity factor is the largest, reaching 54.77, indicating that the contact synergistic effect of this mixed agent on thrips is the most obvious indoors. The mixture of neem and nicotine with a ratio of 1:2 has the highest contact activity on thrips.

Example 3. Binary mixing of botanical pesticide matrine and other 7 kinds of original drugs according to the volume ratio of 5:1, 2:1, 1:1, 1:2, 1:5 to obtain a mixed drug. Investigate the indoor toxicity determination of 7 kinds of mixed medicines to thrips, and use the method of soaking leaves with medicine film for determination, and the specific steps are the same as in Example 1.

Table 3 shows the combined toxicity test results of the above seven mixed agents.

Table 3 Toxicity determination of binary mixture of matrine and other 7 kinds of botanical agents

It can be seen from the data in Table 3 that matrine:nicotine=1:5～5:1 is mixed, and the co-toxicity factors are all greater than 20, indicating that the mixture has a synergistic effect, so when matrine and nicotine After the base is compounded according to this ratio, the effect is significantly higher than that of each single agent. Especially when matrine: nicotine = 5:1, the maximum co-toxicity factor is 48.03, which shows that its mixed synergistic effect is the most significant, and its activity on thrips is the most significant.

Combining Table 2 and Table 3, after a large number of compound screening, it can be found that nicotine has a significant synergistic effect in the control of Lycium barbarum thrips, so nicotine and other 8 kinds of botanical agents were compounded.

Example 4, Indoor toxicity determination of nicotine and other 8 kinds of botanical agents

Table 4 Toxicity determination of binary mixtures of nicotine and other 6 kinds of botanical agents

It can be concluded from the data in Table 4 that nicotine: Fructus Cnidii=1:5～5:1, and the co-toxicity factors are all greater than 20, indicating that the mixture has a synergistic effect; nicotine: Neem=1:5～5 : 1, the co-toxicity factors are all greater than 20, showing that its mixing has a synergistic effect; : Veratrine = 1:5～5:1, the co-toxicity factors are all greater than 20, indicating that its mixing has a synergistic effect; , indicating that its compounding has a synergistic effect; nicotine: crotonine = 1:5 to 5:1, and the co-toxicity factors are all greater than 20, indicating that its compounding has a synergistic effect, so during the entire compounding process, nicotine Alkali plays a synergistic effect, and the effect of compounding with other 8 kinds of agents to prevent and treat thrips is higher than that of each single agent.

Combining Tables 2, 3, 4 and comprehensive factors such as drug costs and plant resources, the following conclusions can be drawn:

The volume percentage of the ingredients: nicotine:neem=2:1, the rest is solvent-water-emulsifier

The volume percentage of the ingredients: nicotine: matrine = 1:5, the rest is water-emulsifier

The volume percentage of the ingredients: nicotine: cnidium = 5:1, the rest is solvent-water-emulsifier

The volume percentage of the ingredients: nicotine: neem=5:1, the rest is water-emulsifier

The volume percentage of the ingredients: nicotine: Echinocarpus = 5:1, the rest is solvent-water-emulsifier

From the above experiments, it can be seen that in view of the serious harm of Lycium barbarum thrips and excessive pesticide residues in Lycium barbarum, a method with significant contact synergistic effect on Lycium barbarum thrips is provided, which provides new technical support for the prevention and control of Lycium barbarum thrips in production. The compounding of nicotine with neem, matrine, cnidium, neem, and bitter bark vine respectively has significant synergistic effect. These 5 kinds of compound preparations: 10 kinds of ratios have higher mortality than wolfberry thrips 95%, when nicotine: neem = 2:1; nicotine and cnidium = 5:1; nicotine: neem = 5:1, 2:1, 1:5; nicotine: neem = 1: At 5 o'clock, the mortality rate reached 100%. In summary, the effects of these 10 compound preparations on preventing and treating thrips were higher than those of each single agent. Combining the indoor toxicity determination and field test of the ratio of 10 kinds of botanical raw materials, the results are as follows: under the condition of 200 times dilution, the indoor toxicity to Lycium barbarum thrips has reached more than 95%; The field control effect reached 85%, 90% after 3 days, 90% after 5 days, and still 90% after 7 days. The prevention effect is remarkable.

detailed description

Embodiment 1: a kind of botanical pesticide preparation of preventing and treating Lycium barbarum thrips, the preparation method of this pesticide preparation is to dissolve the original medicine and solvent into a solution first, then mix the solution into a compound medicinal liquid, and then add the compound medicinal liquid to After the emulsifier is stirred, it becomes the mother liquor, and finally the mother liquor is dripped with water, stirred by high shear, and filtered to obtain the preparation. Any mixture of pyrethrins, azadirachtin or Cnidium; the mass concentration of the original drug is successively 15% of matrine, 6% of picroderm, 1% of azadirachtin, 2% of cnidine, and veratrum Alkali 5%, pyrethrin 25%, azadirachtin 5%, nicotine 95%, cnidium 30%, the concentration of the solution to be mixed obtained after the dilution of the former medicine is sequentially neem 0.01g/L, matrine 0.01 g/L, nicotine 0.03g/L, pyrethrum 0.08g/L, cnidium concentration 0.05g/L, bitter bark 3g/L, neem 0.05g/L, veratrine 0.35g/L, crotonine 20g /L.

The mixed volume ratio of the solution is nicotine: neem=1:5, and the preparation is to make up the mixed solution+20% solvent+8% emulsifier and pure water to 100%;

Embodiment 2: all the other are identical with embodiment 1

The mixed volume ratio of the solution is nicotine:neem=5:1, and the preparation is to make up the mixed solution+20% solvent+8% emulsifier and pure water to 100%;

Embodiment 3: all the other are identical with embodiment 1

The mixed volume ratio of the solution is nicotine:matrine=1:5, and the preparation is to make up the mixed solution+20% solvent+8% emulsifier and pure water to 100%;

Embodiment 4: all the other are identical with embodiment 1

The mixed volume ratio of the solution is nicotine:matrine=5:1, and the preparation is to make up the mixed solution+20% solvent+8% emulsifier and pure water to 100%;

Embodiment 5: all the other are identical with embodiment 1

The mixed volume ratio of the solution is nicotine: Fructus Cnidii=1:5, and the preparation is to make up the mixed solution+25% solvent+8% emulsifier and pure water to 100%;

Embodiment 6: all the other are identical with embodiment 1

The mixed volume ratio of the solution is nicotine: Fructus Cnidii=5:1, and the preparation is to make up the mixed solution+25% solvent+8% emulsifier and pure water to 100%;

Embodiment 7: all the other are identical with embodiment 1

The mixed volume ratio of the solution is nicotine: neem=1:5, and the preparation is made up to 100% of the mixed solution+18% solvent+8% emulsifier and pure water;

Embodiment 8: all the other are identical with embodiment 1

The mixed volume ratio of the solution is nicotine: neem=5:1, and the preparation is mixed solution + 18% solvent + 8% emulsifier, and pure water to make up to 100%;

Embodiment 9: all the other are identical with embodiment 1

The mixed volume ratio of the solution is nicotine: Echinocarpus = 1:5, and the preparation is made up to 100% by adding the mixed solution + 35% solvent + 8% emulsifier, and pure water;

Embodiment 10: all the other are identical with embodiment 1

The mixed volume ratio of the solution is nicotine: Echinocarpus = 5:1, and the preparation is the mixed solution + 35% solvent + 8% emulsifier, and pure water to make up to 100%.

The above-mentioned solvent is acetone, alcohol or water, and the emulsifier is concentrated milk 500, OP-10 or Tween 80.

Claims (2)

1. A botanical pesticide preparation for preventing and treating Lycium barbarum thrips is characterized in that: the preparation method of the pesticide preparation is that the former drug plus solvent is dissolved into a solution, then the solution is mixed into a compound liquid, and then the compound drug The liquid is added with an emulsifier and stirred to become a mother liquor. Finally, the mother liquor is dripped with water, stirred by high shear, and filtered to obtain a preparation. A mixture, the mass concentration of the former medicine is successively 15% of matrine, 6% of picroderm, 1% of azadirachtin, 5% of azadirachtin, 95% of nicotine, and 30% of Fructus Cnidii. After dilution, the concentration of the solution to be mixed is obtained successively as neem 0.01g/L, matrine 0.01g/L, nicotine 0.03g/L, cnidium concentration 0.05g/L, bitter bark 3g/L, neem 0.05g /L, the mixed volume ratio of the solution is: nicotine: Neem=2:1, nicotine: Matrine=1:5, nicotine: Fructus Cnidii=5:1, nicotine: Neem=1: 5. Nicotine: Phyllostachys chinensis=5:1, and the formula of the preparation is any one of the following: The volume ratio is: nicotine:neem=2:1+20% solvent+8% emulsifier, pure water to make up to 100%; The volume ratio is: nicotine: matrine = 1:5 + 20% solvent + 8% emulsifier, pure water to make up to 100%; The volume ratio is: nicotine: cnidium = 5:1 + 25% solvent + 8% emulsifier, pure water to make up to 100%; The volume ratio is: nicotine: neem = 1:5 + 18% solvent + 8% emulsifier, pure water to make up to 100%; The volume ratio is: nicotine: bitter bark vine = 5:1 + 35% solvent + 8% emulsifier, pure water to make up to 100% The above-mentioned content is volume percentage content. 2. A kind of botanical pesticide for preventing and treating wolfberry thrips as claimed in claim 1, characterized in that: the solvent is acetone, alcohol or water, and the emulsifier is concentrated milk 500, OP-10 or Tween 80 .