CN104430517B - A kind of sweet basil salt resistance seed soaking agent and preparation method thereof - Google Patents
A kind of sweet basil salt resistance seed soaking agent and preparation method thereof Download PDFInfo
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- CN104430517B CN104430517B CN201410687253.0A CN201410687253A CN104430517B CN 104430517 B CN104430517 B CN 104430517B CN 201410687253 A CN201410687253 A CN 201410687253A CN 104430517 B CN104430517 B CN 104430517B
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- 235000010676 Ocimum basilicum Nutrition 0.000 title claims abstract description 58
- 238000002791 soaking Methods 0.000 title claims abstract description 45
- 150000003839 salts Chemical class 0.000 title claims abstract description 42
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 240000007926 Ocimum gratissimum Species 0.000 title claims abstract 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 52
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 41
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 36
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 31
- 229930006000 Sucrose Natural products 0.000 claims abstract description 20
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 20
- 239000005720 sucrose Substances 0.000 claims abstract description 20
- IXVMHGVQKLDRKH-YEJCTVDLSA-N (22s,23s)-epibrassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@H](O)[C@@H](O)[C@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-YEJCTVDLSA-N 0.000 claims abstract description 19
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 claims abstract description 19
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 18
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 18
- 239000011718 vitamin C Substances 0.000 claims abstract description 18
- JLKDVMWYMMLWTI-UHFFFAOYSA-M potassium iodate Chemical compound [K+].[O-]I(=O)=O JLKDVMWYMMLWTI-UHFFFAOYSA-M 0.000 claims abstract description 13
- 239000001230 potassium iodate Substances 0.000 claims abstract description 13
- 235000006666 potassium iodate Nutrition 0.000 claims abstract description 13
- 229940093930 potassium iodate Drugs 0.000 claims abstract description 13
- 239000012452 mother liquor Substances 0.000 claims description 7
- 239000002904 solvent Substances 0.000 claims description 6
- -1 10ml/L Substances 0.000 claims description 2
- 150000002148 esters Chemical class 0.000 claims 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 10
- 231100000331 toxic Toxicity 0.000 abstract description 6
- 230000002588 toxic effect Effects 0.000 abstract description 6
- 230000007226 seed germination Effects 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 abstract description 2
- 244000180308 Ocimum basilicum Species 0.000 description 50
- 230000035882 stress Effects 0.000 description 17
- 239000000243 solution Substances 0.000 description 16
- 241000196324 Embryophyta Species 0.000 description 15
- 235000019441 ethanol Nutrition 0.000 description 12
- 230000035784 germination Effects 0.000 description 11
- 239000013535 sea water Substances 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 4
- 239000011575 calcium Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000008635 plant growth Effects 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 229910002651 NO3 Inorganic materials 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000015784 hyperosmotic salinity response Effects 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 1
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 208000006877 Insect Bites and Stings Diseases 0.000 description 1
- 241000207923 Lamiaceae Species 0.000 description 1
- 208000037093 Menstruation Disturbances Diseases 0.000 description 1
- 206010027339 Menstruation irregular Diseases 0.000 description 1
- 235000004072 Ocimum sanctum Nutrition 0.000 description 1
- 240000002837 Ocimum tenuiflorum Species 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 208000004078 Snake Bites Diseases 0.000 description 1
- 208000024780 Urticaria Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 241001062995 Ziziphora Species 0.000 description 1
- 230000036579 abiotic stress Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
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- 206010016766 flatulence Diseases 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
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- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 238000005325 percolation Methods 0.000 description 1
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- 230000001105 regulatory effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 229960004889 salicylic acid Drugs 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 230000008736 traumatic injury Effects 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
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- 229940088594 vitamin Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of sweet basil salt resistance seed soaking agent and preparation method thereof, it is made up of vitamin C, calcium nitrate, sucrose, Potassiumiodate, brassinosteroid and second alcohol and water, and wherein each component mass concentration in the solution is respectively：Vitamin C：0.2 1.5g/L, calcium nitrate：3 15g/L, sucrose：0.5 5g/L, Potassiumiodate：5 22g/L, brassinosteroid：0.02 0.5g/L, ethanol：5 10ml/L, remaining be water.Sweet basil salt resistance seed soaking agent reasonable mixture ratio of the present invention, safe without toxic side effect, ocimi seeds are sprouted, improve percentage of seedgermination under effectively facilitating salt stress.
Description
Technical field
The present invention relates to field of plant growing technology, and in particular to a kind of sweet basil salt resistance seed soaking agent and preparation method thereof.
Background technology
Salinity is one of abiotic stress factor of limiting plant growth and output increased.According to statistics, there are about in the world
23% anthropogenic soil it is different degrees of by saliferous harm, the current saline alkali land area of China is about 99,130,000 hm2, and by
Being presented in the unreasonable development soil salinization increases situation.Wang Yuping etc. sprouts in salicylic acid to cauliflower seed under salt stress
The influence one of hair and Physiological Characteristics the article pointed out：Response of the plant in different growth phases to salt stress has different
Property, and Their Seed Germinating Period is salt resistance ability relatively weak period.For the plantation of crop, germination quality is straight
Connect influence plant growth and economic benefit.Salt marsh environment suppresses seed moisture content and absorbs, and reduces cell permeability, so as to all to seed
Many physiology, biochemical metabolism effect produce toxic effect.
Sweet basil (Ocimum Basilicum L.), is annual Labiatae also known as south Sinkiang ziziphora herb, FRUCTUS OCIMI, holy basil etc.
Herbaceous plant, be distributed widely in Asia, Africa and Central and South America tropical and subtropical region, the Guangdong of China, Guangxi,
There is cultivation on the ground such as Henan, Sichuan, Fujian.Sweet basil is a kind of important fragrant plant, there is a dispelling wind promoting the circulation of qi, dampness elimination helps digestion, invigorates blood circulation,
The function of removing toxic substances.It is wet for exogenous headache, stagnant food flatulence, gastral cavity pain, diarrhea, irregular menstruation, traumatic injury, snake bite and insect sting, skin
The treatment of the diseases such as sore, urticaria sufferings, great edible and medical value；Its blade and the Basil Essential of stem's glandular secretion are used extensively
Make the fields such as flavor enhancement and medicine and the industry of food service industry.Research shows that salt stress significantly inhibits ocimi seeds sprouting.Cause
This, alleviates injury effect of the salt stress to sweet basil sprouting stage, percentage of seedgermination is improved, for improving sweet basil yield and its economic benefit
Play an important roll.Current existing ocimi seeds seed soaking agent is single compound, and only for sweet basil under normal condition
The sprouting of seed has certain promotion, and rarely has for the multiple compounds that ocimi seeds germination percentage is improved under salt marsh environment
The seed soaking agent of combination.
The content of the invention
In order to overcome the deficiencies in the prior art, it is an object of the invention to provide a kind of sweet basil salt resistance seed soaking agent, proportioning is closed
Reason, safe without toxic side effect, ocimi seeds are sprouted, improve percentage of seedgermination under being used to promote salt stress.
Promote to sprout the preparation method of seed soaking agent another object of the present invention is to provide a kind of sweet basil salt resistance, by control group
The order of addition divided, promotees to sprout seed soaking agent to obtain a kind of reasonable mixture ratio, the salt resistance of safe without toxic side effect sweet basil.
To solve the above problems, the technical solution adopted in the present invention is as follows：
A kind of sweet basil salt resistance seed soaking agent, it is by vitamin C, calcium nitrate, sucrose, Potassiumiodate, brassinosteroid and second
Alcohol and water is constituted, and wherein each component mass concentration in the solution is respectively：
Vitamin C：0.2-1.5g/L
Calcium nitrate：3-15g/L
Sucrose：0.5-5g/L
Potassiumiodate：5-22g/L
Brassinosteroid：0.02-0.5g/L
Ethanol：5-10ml/L,
Solvent is water.
Used as preferred scheme, in above-mentioned sweet basil salt resistance seed soaking agent, each component mass concentration in the solution is respectively：
Vitamin C：0.26-1.23g/L
Calcium nitrate：3.28-13.12g/L
Sucrose：0.51-3.09g/L
Potassiumiodate：5.35-21.4g/L
Brassinosteroid：0.024-0.48g/L
Ethanol：5-10ml/L,
Solvent is water.
Used as preferred scheme, in above-mentioned sweet basil salt resistance seed soaking agent, each component mass concentration in the solution is respectively：Dimension
Raw element C0.88g/L；Calcium nitrate 13.12g/L；Sucrose 0.51g/L；Potassiumiodate 21.4g/L；Brassinosteroid 0.48g/L；Ethanol
10ml/L, solvent is water.
In such scheme, specifically, the ethanol or absolute ethyl alcohol of the alcohol 95 %.
In the present invention, calcium nitrate Ca (NO3)2Middle Ca2+MDA and the electrolysis in salt-stressed plants body can effectively be reduced
Matter percolation ratio, suppresses Na+Absorption, maintain plant cell film integrality and stability, reduce environment salt stress toxic effect.
Ca(NO3)2Middle NO3 -With Cl-There is antagonism, i.e. NO during plant absorption3 -Ion can effectively reduce Cl-Uptake, enters
And reduce Cl-Toxic action.Under salt stress, a large amount of Na+Plant is significantly inhibited to K+Absorption, the present invention in Potassiumiodate (KIO3)
K is provided+, can effectively alleviate Na+With K+Competitive absorption, improve plant in K/Na ratios, maintain ionic equilibrium, enter
And improve plant salt tolerance；IO3 -Containing for antioxidase and non-enzyme polyphenoils in Plants under Salt Stress body can be effectively improved
Amount, removes the active oxygen produced in Plants under Salt Stress body, improves plant salt tolerance ability.Vitamin C is antioxidant, and sucrose is
The signaling molecule of regulating growth of plants, brassinosteroid is a kind of chemistry and life.Under salt stress, by vitamin C, sucrose
Or brassinosteroid is used in combination, activities of antioxidant enzymes in plant is remarkably improved, reduces content of oxygen free radical, promote plant
Growth, increases resistance of the plant to salinity.
A kind of preparation method of sweet basil salt resistance seed soaking agent, its step is as follows：
1) vitamin C is weighed soluble in water；
2) weigh that sucrose is soluble in water to be dissolved in step 1) solution in；
3) in step 2) solution in add calcium nitrate, Potassiumiodate to be made seed soaking agent mother liquor；
4) brassinosteroid is weighed to be dissolved in ethanol, and with step 3) mother liquor mixed；
5) solution is finally settled to target volume with water.
Compared to existing technology, the beneficial effects of the present invention are：
1. sweet basil salt resistance seed soaking agent of the present invention is applied to the sweet basil production cultivation of saline soil area, reduces salt stress
Harm, efficiently solve under salt marsh environment that ocimi seeds germination is slow, germination percentage is low, even ablastous problem；
2. soaked seed before being broadcast to seed using sweet basil salt resistance seed soaking agent of the present invention, accelerate ocimi seeds speed of germination, carried
Bud rate occurred frequently；
3. sweet basil salt resistance of the present invention seed soaking agent prescription rationally, have no toxic side effect, preparation method and use operation letter
It is single, easy.
The present invention is described in further detail with specific embodiment below in conjunction with the accompanying drawings.
Brief description of the drawings
Fig. 1 is the using effect comparison diagram of the embodiment of the present invention 1；
Fig. 2 is the using effect comparison diagram of the embodiment of the present invention 2；
Fig. 3 is the using effect comparison diagram of the embodiment of the present invention 3.
Specific embodiment
Embodiment 1
A kind of sweet basil salt resistance seed soaking agent, it is by vitamin C, 0.264g/L；Ca(NO3)2, 8.2g/L；Sucrose, 3.09g/
L；KIO3, 8.56g/L；Brassinosteroid, 0.024g/L；95% ethanol, 5ml；Remaining is constituted for water.
Its preparation method is：1. vitamin C is weighed soluble in water；2. sucrose is weighed soluble in water；3. Ca (NO are added3)2、
KIO3It is made seed soaking agent mother liquor；4. weigh brassinosteroid and be dissolved in 95% ethanol solution, and mixed with above solution；5. most
Solution is settled to target volume with water afterwards.
Application method is as follows：It is diluted with water when in use, extension rate is 50 times of sweet basil salt resistance seed soaking agent.
Comparative example 1
Under 30% seawater stress, the sweet basil to clore basil, three kinds of different cultivars of purple sweet basil and great Ye sweet basils uses implementation
The seed soaking agent seed soaking of example 1, and the every kind of sweet basil of correspondence is cooked a blank for not using the seed soaking agent of above-described embodiment 1；Experiment
Result is referring to Fig. 1.
The result of Fig. 1 shows, after being soaked seed using above-mentioned seed soaking agent, under 30% seawater stress, clore basil, purple sweet basil and
Great Ye sweet basil germination percentages are respectively 58%, 59% and 50%, and 41%, 47% and 55% is respectively increased compared to control group germination percentage,
Seed germinating time shifts to an earlier date 2 days.
Embodiment 2
A kind of sweet basil salt resistance seed soaking agent, it is by vitamin, 1.23g/L；Ca(NO3)23.28g/L；Sucrose 2.0g/L；
KIO3, 5.35g/L；Brassinosteroid, 0.24g/L；95% ethanol, 8ml；Remaining is constituted for water.
Its preparation method is：1. vitamin C is weighed soluble in water；2. sucrose is weighed soluble in water；3. Ca (NO are added3)2、
KIO3It is made seed soaking agent mother liquor；4. weigh brassinosteroid and be dissolved in ethanol solution, and mixed with above solution；5. most
Solution is settled to target volume with water afterwards.
Its application method is as follows：It is diluted with water when in use, extension rate is 80 times of sweet basil salt resistance seed soaking agent.
Comparative example 2
Under 30% seawater stress, the sweet basil to clore basil, three kinds of different cultivars of purple sweet basil and great Ye sweet basils uses implementation
The seed soaking agent seed soaking of example 2, and the every kind of sweet basil of correspondence is cooked a blank for not using the seed soaking agent of above-described embodiment 2；Experiment
Result is referring to Fig. 2.
Fig. 2 results show：After being soaked seed using the seed soaking agent of embodiment 2, under 30% seawater stress, clore basil, purple sweet basil
58%, 59% and 50% is respectively with great Ye sweet basil germination percentages, 35%, 50% and is respectively increased compared to control group germination percentage
60%, seed germinating time shifts to an earlier date 2 days.
Embodiment 3
A kind of sweet basil salt resistance seed soaking agent, it is by vitamin C, 0.88g/L；Ca(NO3)2, 13.12g/L；Sucrose, 0.51g/
L；KIO3, 21.4g/L；Brassinosteroid, 0.48g/L；95% ethanol, 10ml；Remaining is constituted for water.
Its preparation method is：1. vitamin C is weighed soluble in water；2. sucrose is weighed soluble in water；3. Ca (NO are added3)2、
KIO3It is made seed soaking agent mother liquor；4. weigh brassinosteroid and be dissolved in 95% ethanol solution, and mixed with above solution；5. most
Solution is settled to target volume with water afterwards.
Application method is as follows：When in use plus running water dilution, extension rate is 100 times of sweet basil salt resistance seed soaking agent.
Comparative example 3
Under 30% seawater stress, the sweet basil to clore basil, three kinds of different cultivars of purple sweet basil and great Ye sweet basils uses implementation
The seed soaking agent seed soaking of example 3, and the every kind of sweet basil of correspondence is cooked a blank for not using the seed soaking agent of above-described embodiment 3；Experiment
Result is referring to Fig. 3.
Fig. 3 results show：After being soaked seed using the seed soaking agent of embodiment 3, under 30% seawater stress, clore basil, purple sweet basil
58%, 59% and 50% is respectively with great Ye sweet basil germination percentages, 45%, 51% and is respectively increased compared to control group germination percentage
54%, seed germinating time shifts to an earlier date 3 days.
Above-mentioned implementation method is only the preferred embodiment of the present invention, it is impossible to limit the scope of protection of the invention with this,
The change and replacement of any unsubstantiality that those skilled in the art is done on the basis of of the invention belong to institute of the present invention
Claimed scope.
Claims (5)
1. a kind of sweet basil salt resistance seed soaking agent, it is characterised in that it is by vitamin C, calcium nitrate, sucrose, Potassiumiodate, rape element
Ester and second alcohol and water are constituted, and wherein each component mass concentration in the solution is respectively：
Vitamin C：0.2-1.5g/L
Calcium nitrate：3-15g/L
Sucrose：0.5-5g/L
Potassiumiodate：5-22g/L
Brassinosteroid：0.02-0.5g/L
Ethanol：5-10ml/L,
Solvent is water.
2. sweet basil salt resistance seed soaking agent according to claim 1, it is characterised in that wherein each component quality in the solution is dense
Degree is respectively：
Vitamin C：0.26-1.23g/L
Calcium nitrate：3.28-13.12g/L
Sucrose：0.51-3.09g/L
Potassiumiodate：5.35-21.4g/L
Brassinosteroid：0.024-0.48g/L
Ethanol：5-10ml/L,
Solvent is water.
3. sweet basil salt resistance seed soaking agent according to claim 1, it is characterised in that wherein each component quality in the solution is dense
Degree is respectively：
Vitamin C 0.88g/L；Calcium nitrate 13.12g/L；Sucrose 0.51g/L；Potassiumiodate 21.4g/L；Brassinosteroid 0.48g/
L；Ethanol, 10ml/L, solvent is water.
4. sweet basil salt resistance seed soaking agent according to claim 1, it is characterised in that the ethanol is 95% ethanol or nothing
Water-ethanol.
5. a kind of preparation method of sweet basil salt resistance seed soaking agent as claimed in claim 1, it is characterised in that its step is as follows：
1) vitamin C is weighed soluble in water；
2) weigh that sucrose is soluble in water to be dissolved in step 1) solution in；
3) in step 2) solution in add calcium nitrate, Potassiumiodate to be made seed soaking agent mother liquor；
4) brassinosteroid is weighed to be dissolved in ethanol, and with step 3) mother liquor mixed；
5) solution is finally settled to target volume with water.
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| WO2018123651A1 (en) * | 2016-12-27 | 2018-07-05 | 国立研究開発法人理化学研究所 | Agent for improving environmental stress tolerance of plant |
| CN110074114A (en) * | 2019-04-29 | 2019-08-02 | 天津市汉邦植物保护剂有限责任公司 | A kind of brassinolide-containing, aqua of sugar alcohol calcium and preparation method and application |
| CN115245175B (en) * | 2022-08-30 | 2023-10-10 | 淮阴师范学院 | A kind of seed treatment agent and preparation method and application thereof |
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| CN103798285A (en) * | 2014-01-24 | 2014-05-21 | 中国农业科学院草原研究所 | Alfalfa seed soaking liquid and related method |
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| CN101375688A (en) * | 2008-09-01 | 2009-03-04 | 山东省农业科学院作物研究所 | Salt-resistance formulation for improving salt resistance of wheat and method of use thereof |
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| 维生素C浸种对盐胁迫下紫花苜蓿种子发芽特性的影响;申玉华 等;《种子》;20090731;第28卷(第7期);第42-44页 * |
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