CN104351258A - Compound biopesticide for preventing and curing rice sheath blight disease and preparation method thereof - Google Patents
Compound biopesticide for preventing and curing rice sheath blight disease and preparation method thereof Download PDFInfo
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- CN104351258A CN104351258A CN201410557569.8A CN201410557569A CN104351258A CN 104351258 A CN104351258 A CN 104351258A CN 201410557569 A CN201410557569 A CN 201410557569A CN 104351258 A CN104351258 A CN 104351258A
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- sheath blight
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 55
- 235000009566 rice Nutrition 0.000 title claims abstract description 55
- 238000002360 preparation method Methods 0.000 title claims abstract description 35
- 150000001875 compounds Chemical class 0.000 title abstract description 25
- 230000000853 biopesticidal effect Effects 0.000 title abstract description 11
- 201000010099 disease Diseases 0.000 title abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title abstract description 7
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 54
- 241001465752 Purpureocillium lilacinum Species 0.000 claims abstract description 41
- 239000000575 pesticide Substances 0.000 claims abstract description 41
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 39
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 39
- 230000001580 bacterial effect Effects 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 9
- 238000009472 formulation Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 claims description 2
- 230000004151 fermentation Effects 0.000 claims description 2
- 238000012216 screening Methods 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 239000003905 agrochemical Substances 0.000 claims 11
- 230000002265 prevention Effects 0.000 claims 10
- 239000000463 material Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 11
- 239000003337 fertilizer Substances 0.000 abstract description 6
- 239000000843 powder Substances 0.000 abstract description 4
- 239000002689 soil Substances 0.000 abstract description 3
- 239000002881 soil fertilizer Substances 0.000 abstract description 3
- 239000002207 metabolite Substances 0.000 abstract description 2
- 230000002195 synergetic effect Effects 0.000 abstract description 2
- 239000007864 aqueous solution Substances 0.000 abstract 2
- 241000108664 Nitrobacteria Species 0.000 abstract 1
- 239000004480 active ingredient Substances 0.000 abstract 1
- 239000002994 raw material Substances 0.000 description 9
- 239000000126 substance Substances 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- 239000003814 drug Substances 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- WUOACPNHFRMFPN-VIFPVBQESA-N (R)-(+)-alpha-terpineol Chemical compound CC1=CC[C@H](C(C)(C)O)CC1 WUOACPNHFRMFPN-VIFPVBQESA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 244000178870 Lavandula angustifolia Species 0.000 description 1
- 235000010663 Lavandula angustifolia Nutrition 0.000 description 1
- 241000270708 Testudinidae Species 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000001102 lavandula vera Substances 0.000 description 1
- 235000018219 lavender Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000001546 nitrifying effect Effects 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract
Description
技术领域technical field
本发明涉及生物农药及其制备方法，特别是一种防治水稻纹枯病的复配生物农药及其制备方法。The invention relates to a biological pesticide and a preparation method thereof, in particular to a compound biological pesticide for preventing and treating rice sheath blight and a preparation method thereof.
背景技术Background technique
水稻纹枯病是水稻的三大病害之一，其在世界上分布广，危害大，该病不但影响水稻的产量，而且还严重影响水稻的稻米质量。至今该病害已在亚洲、美洲、非洲等种植水稻的国家普遍发生，针对水稻纹枯病已有一些防治措施。查阅相关文献，中国专利公开(公告)号：CN103396954A，公开了一种防控水稻纹枯病的生物防治菌株、生物有机肥及其制备方法，利用棘孢木霉菌株12发酵液制备一种生物肥料，发现其对水稻纹枯病的防治率为70％。中国专利公开(公告)号：CN103719162A，公开了一种防治水稻纹枯病的药物及其生产方法，利用金线吊乌龟块根提取物与乙蒜素和左旋α-松油醇常温下合并，发现药物一次喷洒水稻纹枯病防治率达到70％。以上公开的是对水稻文枯病的防治率仅达到70％，为功效单一的生物农药。Rice sheath blight is one of the three major diseases of rice. It is widely distributed in the world and is very harmful. This disease not only affects the yield of rice, but also seriously affects the quality of rice. So far, the disease has generally occurred in rice-growing countries in Asia, America, and Africa, and some control measures have been taken for rice sheath blight. Consult related documents, Chinese Patent Publication (Announcement) No.: CN103396954A, discloses a kind of biological control bacterial strain for preventing and controlling rice sheath blight, biological organic fertilizer and preparation method thereof, utilizes Trichoderma aculeatus strain 12 fermented liquid to prepare a biological fertilizer, it was found that its control rate against rice sheath blight was 70%. Chinese Patent Publication (Announcement) No.: CN103719162A discloses a medicine for preventing and treating rice sheath blight and a production method thereof. The root tuber extract of the golden-haired tortoise is combined with acetoallin and L-α-terpineol at room temperature, and it is found that The control rate of rice sheath blight can reach 70% by spraying medicine once. What is disclosed above is that the control rate of rice bacterial blight only reaches 70%, and it is a biological pesticide with a single effect.
发明内容Contents of the invention
本发明提供了一种防治水稻纹枯病的复配生物农药及其制备方法，该农药同时可以提高土壤肥效，是肥药双效的生物农药。The invention provides a compound biological pesticide for preventing and treating rice sheath blight and a preparation method thereof. The pesticide can improve soil fertilizer efficiency at the same time, and is a biological pesticide with double effects of fertilizer and medicine.
一种防治水稻纹枯病的复配生物农药，其特征是该农药的剂型为水剂或粉剂，水剂或粉剂中活性组分为：枯草芽孢杆菌B-2103和淡紫拟青霉BS-003，枯草芽孢杆菌B-2103含量为：500-8000CFU/μL；淡紫拟青霉BS-003含量为：1000-10000CFU/μL。A compound biopesticide for preventing and treating rice sheath blight is characterized in that the formulation of the pesticide is water or powder, and the active components in the water or powder are: Bacillus subtilis B-2103 and Paecilomyces lilacinus BS- 003, the content of Bacillus subtilis B-2103: 500-8000 CFU/μL; the content of Paecilomyces lilacinus BS-003: 1000-10000 CFU/μL.
所述的一种防治水稻纹枯病的复配生物农药，其特征是农药剂型为水剂，水剂中活性组分为：枯草芽孢杆菌B-2103和淡紫拟青霉BS-003，枯草芽孢杆菌B-2103含量为：1000-6000CFU/μL；淡紫拟青霉BS-003含量为：500-6000CFU/μL。The compound biopesticide for preventing and treating rice sheath blight is characterized in that the pesticide dosage form is water preparation, and the active components in the water preparation are: Bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003, subtilis The content of Bacillus B-2103: 1000-6000 CFU/μL; the content of Paecilomyces lilacinus BS-003: 500-6000 CFU/μL.
所述的淡紫拟青霉BS-003是经发酵、分离筛选得到的活菌制剂。The Paecilomyces lilacinus BS-003 is a live fungal preparation obtained through fermentation, separation and screening.
一种防治水稻纹枯病的复配生物农药的制备方法，其特征是该方法采用的农药原料是枯草芽孢杆菌B-2103和淡紫拟青霉BS-003菌株，枯草芽孢杆菌B-2103与淡紫拟青霉BS-003菌株的重量配比为1∶0.0005-1000。A method for preparing compound biological pesticides for preventing and treating rice sheath blight, characterized in that the pesticide raw materials used in the method are Bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 strains, and Bacillus subtilis B-2103 and The weight ratio of Paecilomyces lilacinus BS-003 strain is 1:0.0005-1000.
所述一种防治水稻纹枯病的复配生物农药的制备方法，其特征是枯草芽孢杆菌B-2103及淡紫拟青霉BS-003的重量配比为1∶0.005-0.01。The preparation method of the compound biological pesticide for preventing and treating rice sheath blight is characterized in that the weight ratio of Bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 is 1:0.005-0.01.
所述一种防治水稻纹枯病的复配生物农药的制备方法，其特征是枯草芽孢杆菌B-2103及淡紫拟青霉BS-003的重量配比为1∶0.05-0.1。The preparation method of the compound biological pesticide for preventing and controlling rice sheath blight is characterized in that the weight ratio of Bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 is 1:0.05-0.1.
所述一种防治水稻纹枯病的复配生物农药的制备方法，其特征是枯草芽孢杆菌B-2103及淡紫拟青霉BS-003的重量配比为1∶0.2-1。The preparation method of the compound biological pesticide for preventing and controlling rice sheath blight is characterized in that the weight ratio of Bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 is 1:0.2-1.
所述一种防治水稻纹枯病的复配生物农药的制备方法，其特征是枯草芽孢杆菌B-2103及淡紫拟青霉BS-003的重量配比为1∶5-50。The preparation method of the compound biological pesticide for preventing and controlling rice sheath blight is characterized in that the weight ratio of Bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 is 1:5-50.
所述一种防治水稻纹枯病的复配生物农药的制备方法，其特征是枯草芽孢杆菌B-2103及淡紫拟青霉BS-003的重量配比为1∶60-100。The preparation method of the compound biological pesticide for controlling rice sheath blight is characterized in that the weight ratio of Bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 is 1:60-100.
所述一种防治水稻纹枯病的复配生物农药的制备方法，其特征是枯草芽孢杆菌B-2103及淡紫拟青霉BS-003的重量配比为1∶100-300。The preparation method of the compound biological pesticide for preventing and controlling rice sheath blight is characterized in that the weight ratio of Bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 is 1:100-300.
本发明与现有同类技术相比，其显著的有益效果体现在：Compared with existing similar technologies, the present invention has remarkable beneficial effects embodied in:
本发明采用淡紫拟青霉BS-003菌株及枯草芽孢杆菌B-2103为农药原料，由于这两种生物原料的作用机理不同，通过二者复配，胞外代谢产物对水稻纹枯病菌及硝化细菌等防效良好，可以发挥协同效应，降低用药量，减少抗药性发生。对水稻纹枯病的防治率达82％以上，同时可以提高土壤中肥效，增强土壤N肥利用效率。The present invention adopts Paecilomyces lilacinus BS-003 bacterial strain and Bacillus subtilis B-2103 as pesticide raw materials, because the mechanism of action of these two kinds of biological raw materials is different, through the compounding of the two, extracellular metabolites are effective against rice sheath blight bacteria. And nitrifying bacteria, etc. have good control effects, can exert a synergistic effect, reduce the dosage of drugs, and reduce the occurrence of drug resistance. The control rate of rice sheath blight is more than 82%, and at the same time, it can improve the fertilizer efficiency in the soil and enhance the utilization efficiency of soil N fertilizer.
本发明有利于保护农业生态系统；有利于农业可持续发展，农药持效期长，对环境无污染、无残留。The invention is beneficial to the protection of the agricultural ecological system, and is beneficial to the sustainable development of agriculture, the pesticide has a long lasting period, and has no pollution and no residue to the environment.
具体实施方式Detailed ways
以下结合具体实施例来进一步描述本发明，描述涉及的重量百分比均为纯物质的重量百分比。The present invention is further described below in conjunction with specific examples, and the weight percentages involved in the description are all weight percentages of pure substances.
实施例1Example 1
一种防治水稻纹枯病的复配生物农药，该农药的剂型为水剂，水剂中活性组分及含量为：A compound biopesticide for preventing and treating rice sheath blight, the pesticide is in the form of water preparation, and the active components and content in the water preparation are:
枯草芽孢杆菌B-2103：10000CFU/μL；淡紫拟青BS-003：10000CFU/μL。Bacillus subtilis B-2103: 10,000 CFU/μL; Piedaceae lavender BS-003: 10,000 CFU/μL.
一种防治水稻纹枯病的复配生物农药的制备方法，采用的农药原料是枯草芽孢杆菌B-2103和淡紫拟青霉BS-003菌株，其重量配比为1∶0.005-0.01。A method for preparing compound biological pesticides for preventing and controlling rice sheath blight. The pesticide raw materials used are Bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 strains, and the weight ratio is 1:0.005-0.01.
防治水稻纹枯病药剂效果比较表Comparative Table of Effects of Chemicals for Controlling Rice Sheath Blight
实施例2Example 2
一种防治水稻纹枯病的复配生物农药，该农药的剂型为水剂，水剂中活性组分及含量为：A compound biopesticide for preventing and treating rice sheath blight, the pesticide is in the form of water preparation, and the active components and content in the water preparation are:
枯草芽孢杆菌B-2103：10000CFU/μL；淡紫拟青霉BS-003：10000CFU/μL。Bacillus subtilis B-2103: 10000 CFU/μL; Paecilomyces lilacinus BS-003: 10000 CFU/μL.
一种防治水稻纹枯病的复配生物农药的制备方法，采用的农药原料是枯草芽孢杆菌B-2103和淡紫拟青霉BS-003菌株，其重量配比为为1∶0.05-0.1。A method for preparing compound biological pesticides for preventing and controlling rice sheath blight. The pesticide raw materials used are Bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 strains, and the weight ratio is 1:0.05-0.1.
防治水稻纹枯病药剂效果比较表Comparative Table of Effects of Chemicals for Controlling Rice Sheath Blight
实施例3Example 3
一种防治水稻纹枯病的复配生物农药，该农药的剂型为水剂，水剂中活性组分及含量为：A compound biopesticide for preventing and treating rice sheath blight, the pesticide is in the form of water preparation, and the active components and content in the water preparation are:
枯草芽孢杆菌B-2103：10000CFU/μL；淡紫拟青霉BS-003：10000CFU/μL。Bacillus subtilis B-2103: 10000 CFU/μL; Paecilomyces lilacinus BS-003: 10000 CFU/μL.
一种防治水稻纹枯病的复配生物农药的制备方法，采用的农药原料是枯草芽孢杆菌B-2103和淡紫拟青霉BS-003菌株，其重量配比为为1∶0.2-1。A method for preparing compound biological pesticides for preventing and controlling rice sheath blight. The pesticide raw materials used are Bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 strains, and the weight ratio is 1:0.2-1.
防治水稻纹枯病药剂效果比较表Comparative Table of Effects of Chemicals for Controlling Rice Sheath Blight
实施例4Example 4
一种防治水稻纹枯病的复配生物农药，该农药的剂型为水剂，水剂中活性组分及含量为：A compound biopesticide for preventing and treating rice sheath blight, the pesticide is in the form of water preparation, and the active components and content in the water preparation are:
枯草芽孢杆菌B-2103：10000CFU/μL；淡紫拟青霉BS-003：10000CFU/μL。Bacillus subtilis B-2103: 10000 CFU/μL; Paecilomyces lilacinus BS-003: 10000 CFU/μL.
一种防治水稻纹枯病的复配生物农药的制备方法，采用的农药原料是枯草芽孢杆菌B-2103和淡紫拟青霉BS-003菌株，其重量配比为为1∶5-50。The invention discloses a preparation method of a compound biological pesticide for preventing and controlling rice sheath blight. The pesticide raw materials used are Bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 strains, and the weight ratio is 1:5-50.
防治水稻纹枯病药剂效果比较表Comparative Table of Effects of Chemicals for Controlling Rice Sheath Blight
实施例5Example 5
一种防治水稻纹枯病的复配生物农药，该农药的剂型为水剂，水剂中活性组分及含量为：A compound biopesticide for preventing and treating rice sheath blight, the pesticide is in the form of water preparation, and the active components and content in the water preparation are:
枯草芽孢杆菌B-2103：10000CFU/μL；淡紫拟青霉BS-003：10000CFU/μL。Bacillus subtilis B-2103: 10000 CFU/μL; Paecilomyces lilacinus BS-003: 10000 CFU/μL.
一种防治水稻纹枯病的复配生物农药的制备方法，采用的农药原料是枯草芽孢杆菌B-2103和淡紫拟青霉BS-003菌株，其重量配比为为1∶60-100。A method for preparing compound biological pesticides for preventing and controlling rice sheath blight, the pesticide raw materials used are Bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 strains, and the weight ratio is 1:60-100.
防治水稻纹枯病药剂效果比较表Comparative Table of Effects of Chemicals for Controlling Rice Sheath Blight
实施例6Example 6
一种防治水稻纹枯病的复配生物农药，该农药的剂型为水剂，水剂中活性组分及含量为：A compound biopesticide for preventing and treating rice sheath blight, the pesticide is in the form of water preparation, and the active components and content in the water preparation are:
枯草芽孢杆菌B-2103：10000CFU/μL；淡紫拟青霉BS-003：10000CFU/μL。Bacillus subtilis B-2103: 10000 CFU/μL; Paecilomyces lilacinus BS-003: 10000 CFU/μL.
一种防治水稻纹枯病的复配生物农药的制备方法，采用的农药原料是枯草芽孢杆菌B-2103和淡紫拟青霉BS-003菌株，其重量配比为为1∶100-300。A method for preparing compound biological pesticides for preventing and controlling rice sheath blight. The pesticide raw materials used are Bacillus subtilis B-2103 and Paecilomyces lilacinus BS-003 strains, and the weight ratio is 1:100-300.
防治水稻纹枯病药剂效果比较表Comparative Table of Effects of Chemicals for Controlling Rice Sheath Blight
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| CN101671210A (en) * | 2009-10-29 | 2010-03-17 | 福建省农业科学院农业生物资源研究所 | Preparation method of paecilomyces lilacinus biological fertilizer |
| CN101857848A (en) * | 2010-05-10 | 2010-10-13 | 华中农业大学 | Biocontrol Bacillus subtilis WJ-1 of rice sheath blight and its agent and application |
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| WO2014086752A1 (en) * | 2012-12-03 | 2014-06-12 | Bayer Cropscience Ag | Composition comprising biological control agents |
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2014
- 2014-10-20 CN CN201410557569.8A patent/CN104351258A/en active Pending
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| CN101671210A (en) * | 2009-10-29 | 2010-03-17 | 福建省农业科学院农业生物资源研究所 | Preparation method of paecilomyces lilacinus biological fertilizer |
| CN101857848A (en) * | 2010-05-10 | 2010-10-13 | 华中农业大学 | Biocontrol Bacillus subtilis WJ-1 of rice sheath blight and its agent and application |
| CN101935628A (en) * | 2010-06-13 | 2011-01-05 | 浙江大学 | A kind of bacillus subtilis and use thereof |
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Application publication date: 20150218 |