CN104257786B - A kind of coptis external preparation, preparation method and applications - Google Patents

Abstract

The invention provides an external preparation of Coptidis rhizome, which is composed of Coptidis rhizome extract, astringent and surfactant; the invention solves the problem that the water extract of Coptidis rhizome has poor viscosity, is difficult to attach to the skin surface and tends to flow, and increases the number of patients. Comfortable to use; and compared with the unilateral water extract of Coptis chinensis, the anti-inflammatory effect of Coptis chinensis external preparation is better, and it has a good therapeutic effect on eczema and acne; effect.

Description

Coptis rhizome external preparation, preparation method and application thereof

technical field

The invention relates to an external preparation of traditional Chinese medicine, and more specifically relates to an external preparation of Coptis rhizome, a preparation method and application thereof.

technical background

Coptis chinensis clears heat and dries dampness, purifies fire and detoxifies. For damp and hot fullness, vomiting and acid regurgitation, diarrhea, jaundice, high fever and coma, hyperactivity of heart fire, upset and insomnia, vomiting of blood heat, red eyes, toothache, thirst, carbuncle and furuncle; external treatment of eczema, Eczema, ear canal discharge pus. The wine Coptis chinensis is scorched hot. For red eyes, aphtha. Li Hui et al. reported the effect of sitz bath of Huanglian Jiedu Decoction on anal eczema, showing that the shortest 6 days of treatment in this group, the longest 27 days, with an average of 9 to 18 days, resulted in 112 cases of recovery, 44 cases of improvement, and 12 cases of ineffectiveness. Coptidis Jiedu Decoction has satisfactory effect in treating anal eczema with sitz bath. Wang Shuzhong et al. reported the clinical efficacy and safety of berberine wet compress combined with hydrocortisone butyrate ointment in the treatment of acute exudative eczema in infants. Methods: 78 outpatients with acute exudative eczema in infants were randomly divided into an observation group of 42 cases and a control group of 36 cases. The observation group was treated with berberine solution cold wet compress, bid; the control group was treated with 2% boric acid cold wet compress, bid. 5 days each is a course of treatment. After the skin lesions were dry, both groups of children were externally applied hydrocortisone butyrate ointment, bid, 5 days as a course of treatment. The total course of treatment is 10d. Results: The total effective rate of the observation group was 90.47%, and that of the control group was 69.44%. The difference between the two groups was statistically significant (P<0.01); the recovery rate of the observation group was 50.00%, and that of the control group was 38.89%. Statistically significant (P<0.05). Conclusion: Berberine solution combined with hydrocortisone butyrate ointment is effective and safe in the treatment of acute exudative eczema in infants, and it is worthy of clinical application.

Traditional Chinese medicine believes that acne is caused by endogenous damp heat and fire poison, internal stagnation of damp fire, or internal stagnation of seven emotions, stagnation of qi turning into fire, water deficiency of fire, and flaming fire evil, which accumulate in the skin. We use berberine because it has the functions of clearing heat and dampness, detoxifying, cooling blood, purging fire, and reducing inflammation. Berberine can inhibit the reproduction of acne and reduce the free fatty acids in sebum. Li Chunsheng et al. reported the clinical efficacy of Huanglianjiedu Decoction combined with Loquat Leaf Pills in the treatment of acne vulgaris. Method: Treat 60 patients with acne vulgaris with Huanglian Jiedu Decoction and Pipaye Pills, record the changes of local and systemic symptoms before and after taking the medicine, and evaluate its curative effect, emphasizing dietary taboos. Results: Of the 60 patients, 9 were cured (15%), markedly effective in 39 (65%), improved in 7 (11.67%), and unhealed in 5 (8.33%). The effective rate is 80%, the total effective rate is 91.67%, and the recurrence rate is 11.1%. Conclusion: Huanglian Jiedu Decoction combined with Pipaye Pills is one of the effective prescriptions for acne vulgaris. Fasting of dairy products and reducing sugar intake during treatment are beneficial to the cure of this disease.

Contents of the invention

The invention provides a Coptis rhizome external preparation for treating eczema and acne, which is mainly composed of Coptis chinensis extract.

The external preparation of Coptidis rhizome is composed of Coptidis rhizome extract, astringent and surfactant.

The external preparation of Coptis rhizome is characterized in that: the astringent is tannic acid, and the surfactant is composed of soybean lecithin.

The external preparation of Coptis chinensis is characterized in that the weight ratio of the original medicinal material of the Coptis chinensis extract, tannic acid and soybean lecithin is 15-30:0.3-0.6:0.7-1.2.

The external preparation of Coptis chinensis is characterized in that: the weight ratio of the original medicinal material of Coptis chinensis, tannic acid and soybean lecithin in the external preparation is 20:0.5:0.9;

The external preparation of Coptis rhizome is characterized in that: every 100ml of the external preparation contains Coptis chinensis extract weighing 20g of the original medicinal material, 0.5g of tannic acid and 0.9g of soybean lecithin;

The solvent of the external preparation is 2-5% ethanol; it can be made into one of liniment, spray and aerosol.

The external preparation of Coptis rhizome is characterized in that:

The external preparation can support cream, wherein the base of the oil phase is a mixture of stearic acid, glyceryl monostearate, white petrolatum, lanolin and cetyl alcohol; the base of the water phase is a mixture of glycerin, lauryl A mixed solution of sodium alkanoate, ethylparaben and azone.

The above-mentioned preparation method of Coptis rhizome external preparations in the form of liniment, spray and aerosol is characterized in that it comprises the following steps:

1) Pulverize Coptidis medicinal material, sieve;

2) extracting Coptis chinensis medicinal material with water reflux, cooling, removing impurities, concentrating to 30g of the original medicinal material/100ml, standing for cooling, filtering the precipitate, and obtaining Coptis chinensis water extract;

5) Coptis chinensis extract is clarified twice with UTA-specific chitosan;

3) adding ethanol to the water extract of Coptidis rhizome, adjusting the alcohol concentration to 50-60%, standing and filtering the precipitate to obtain the clarified solution of Coptidis rhizome;

4) recover ethanol and add water and ethanol solution to adjust solvent ethanol concentration to 2-5%, and each 100ml contains 15-20g Coptidis rhizome extract, to obtain Coptidis rhizome extract;

5) adding tannic acid and soybean lecithin to prepare a Coptis rhizome external preparation.

The above-mentioned formulation is the preparation method of the Coptis rhizome external preparation of emulsifiable paste, comprises the following steps:

1) Pulverize Coptidis medicinal material, sieve;

2) extracting Coptis chinensis medicinal material with water reflux, cooling, removing impurities, concentrating to 30g of the original medicinal material/100ml, standing for cooling, filtering the precipitate, and obtaining Coptis chinensis water extract;

3) Concentrating the water extract of Coptidis rhizome, adding the aqueous matrix;

4) adding tannic acid and soybean lecithin to the oil phase matrix;

5) Mixing the water phase base and the oil phase base to obtain the Danshen combination medicine cream for treating acne.

Application of the external preparation of Coptis rhizome in preparation of medicine for treating eczema.

Application of the external preparation of Coptidis rhizoma in preparation of medicine for treating acne.

The beneficial technical effect of the present invention is:

First, the aqueous formulation of Coptidis rhizome for external use solves the problem that the water extract of Coptis chinensis has poor viscosity and is difficult to adhere to the skin surface and is prone to flow, which increases the comfort of patients;

Second, compared with the unilateral water extract of Coptis chinensis, the anti-inflammatory effect of Coptis chinensis topical preparation is better, and it has a good therapeutic effect on eczema and acne;

Thirdly, the preparation has the effect of eliminating scars, and has a good reducing effect on the scars produced by acne.

detailed description

Example 1 Effects of Different Clarification Treatments on Coptis Rhizoma External Preparations

(1) Preparation of Coptis Rhizoma Extract

Crush Coptidis medicinal material, sieve, divide into 3 groups, each group has 3 parts, each part is 100g; extract each part of Coptidis medicinal material with 5 times, 3 times and 3 times of water reflux, each time for 30min, cool, remove impurities, concentrate to 30g of raw medicinal materials/100ml, let it stand for cooling, filter the precipitate to obtain the water extract of Coptis chinensis; add ethanol to the water extract of Coptis chinensis, adjust the alcohol concentration to be greater than 60%, leave it to stand and filter the precipitate to obtain the clarified solution of Coptis chinensis; recover the ethanol and add water and ethanol The solution adjusts the solvent ethanol concentration to 2-5%, and every 100ml contains 30g of Coptis chinensis original medicinal material extract to obtain Coptis chinensis extract.

(2) Investigation of clarification methods

1. Second clarification of chitosan

Weigh chitosan 1g, add 1% acetic acid 100mL, stir magnetically and let stand for 24h, set aside;

Take 1 group (3 parts) of the Coptidis Rhizoma extract obtained in the above steps, heat it to 50°C, add chitosan clarifying agent to each part according to the amount of 5%, 10%, and 20% of the volume of the medicinal solution, and add While stirring at 100r·min ^-1 for 10min, the solution was allowed to cool and then allowed to stand for 12h, centrifuged at 10000r·min ^-1 , filtered, and the acetic acid was evaporated to obtain the chitosan treatment group.

2. ZTC 1+1-Ⅲ clarifier secondary clarification

A component (white to light yellow powder) 1g, add a small amount of distilled water, stir to form a paste, then add distilled water to a sufficient amount, swell for 24 hours, stir to make a 1% solution. Component B (light yellow to light yellow powder) 1g, add a small amount of 1% acetic acid solution, stir to form a paste, add 1% acetic acid solution to a sufficient amount, swell for 24 hours, make a 1% solution, and set aside;

Take 1 group (3 parts) of Coptidis Rhizoma extract obtained in the above steps, heat it to 70°C, add ZTC1+1-Ⅲ clarifying agent according to the amount of 5%, 10%, and 20% of the volume of the medicinal solution, and the sample solution is first After adding component B, add component A, stir at 100r·min ^-1 for 10min while adding, let the solution cool down and let it stand for 12h, centrifuge at 10000r·min ^-1 , filter, and evaporate the acetic acid to obtain the ZTC treatment group.

(3) Effects of different clarification methods on Coptis rhizome external preparations

Add water and ethanol to the untreated Coptidis Rhizoma extract, chitosan treatment group and ZTC treatment group, and every 100ml contains Coptidis Rhizoma extract weighing 20g of the original medicinal material, 0.5g tannic acid and 0.9g soybean lecithin, and the ethanol concentration is 3 -4%. Store at room temperature for 10 days; investigate the clarification effect; details are shown in Table 1.

Table 1 Effects of different secondary clarification methods on Coptis rhizome topical preparations

Coptis chinensis extract contains a large number of complex components such as fine particles, mucus, protein, pectin, starch, etc. These substances together form a colloidal dispersion system of 1-100nm, and it is difficult to remove the above-mentioned substances by using a water extraction and alcohol precipitation system. Adding tannic acid to the extract of Coptidis rhizome will lead to a large amount of precipitation, which will seriously affect the use of external preparations of Coptis chinensis. Therefore, it is very necessary to carry out secondary clarification treatment on the extract of Coptis chinensis.

The above liquid is bottled to make one of spray, liniment or aerosol.

Preparation method of coptis external preparation cream

Weigh 200g of Coptis chinensis, crush the Coptis chinensis, and sieve; extract each portion of Coptis chinensis with 5 times, 3 times, and 3 times of water reflux, each time for 30min, cool, remove impurities, concentrate to 30g of the original drug/100ml, and let it stand Cool and filter the precipitate to obtain the water extract of Coptis chinensis; concentrate to a thick paste, add water phase matrix (117g of glycerin, 18g of sodium laurylate, 1.8g of ethylparaben, and 20g of azone are added to an appropriate amount of water) to dissolve, and dissolve at 80 ℃ heat preservation, spare;

Add 5g of tannic acid and 9g of soybean lecithin into the oil phase base (9g of stearic acid, 36g of glyceryl monostearate, 90g of white petrolatum, 72g of lanolin, and 72g of cetyl alcohol), stir well, and keep warm at 80°C ,spare;

Slowly add the above-mentioned oil phase base into the water phase base, stir 1000r/min while adding, keep at 80°C for 15min, make a cream, cool down to room temperature, and prepare a cream.

Embodiment 3 Different emulsifiers have anti-keratinization effect on rabbit ear acne experimental model

Preparation of experimental samples

Crush Coptis chinensis, sieve, divide into 5 groups, 3 parts in each group, 100g each; extract each part of Coptis chinensis with 5 times, 3 times, 3 times of water reflux, each time for 30min, cool, remove impurities, concentrate to 30g of raw medicinal materials/100ml, let it stand for cooling, filter the precipitate to obtain the water extract of Coptis chinensis; add ethanol to the water extract of Coptis chinensis, adjust the alcohol concentration to 50-60%, let it stand and filter the precipitate to obtain the clarified solution of Coptis chinensis; recover the ethanol and add water and the ethanol solution to adjust the ethanol concentration of the solvent to 2-5%, and each 100ml contains 30g of Coptis chinensis original medicinal material extract to obtain Coptis chinensis extract. Heat each portion of Coptis chinensis extract to 50°C, add chitosan clarifier to each portion according to 5% of the volume of the liquid medicine, stir at 100r·min ^-1 for 10min while adding, let the solution cool down and let it stand for 12h ^, 10000r·min -1 ^1. Centrifuge, filter, and wave off acetic acid to obtain chitosan treatment liquid.

Water was added to the chitosan-treated liquid so that 20 g of the extract of the original medicinal material of Coptidis Rhizoma was contained per 100 ml, and the extract of Coptidis Rhizoma was obtained.

Get wherein one group of chitosan treatment liquids add water, ethanol tannic acid and soybean lecithin, make every 100ml contain the extract of 20g Coptidis rhizome former medicinal material, 0.5g tannic acid and 0.9g soybean lecithin, the concentration of solvent ethanol is 2- 5%; experimental group I was obtained.

Get wherein one group of chitosan treatment liquids add water, ethanol tannic acid and Tween-80, make every 100ml contain the extract of 20g Coptidis rhizome former medicinal material, 0.5g tannic acid and 0.9g Tween-80, the concentration of solvent ethanol is 2-5%; experimental group II was obtained.

Get wherein one group of chitosan treatment liquids add water, ethanol tannic acid and lauric monoglyceride, make every 100ml contain the extract of 20g Coptidis rhizome original medicinal material, 0.5g tannic acid and 0.9g Tween-80, the concentration of solvent ethanol 2-5%; the experimental group III was obtained.

Get wherein one group of chitosan treatment liquids add water and ethanol tannic acid, make every 100ml contain the extract of 20g Coptidis Rhizoma former medicinal material and 0.5g tannic acid, do not contain Coptidis Rhizoma extract, the concentration of solvent ethanol is 2-5%; Obtain Experiment IV group.

Healthy white male rabbits, body weight 2000～2500g, 0.1% retinoic acid cream (, "Chinese Pharmacopoeia" 2010 edition part two); coal tar, black, paste.

Grouping 24 healthy male white rabbits were randomly divided into 8 groups: normal control group, model control group, positive drug control group, coptis extract group, experiment Ⅰ group, experiment Ⅱ group, experiment Ⅲ group and experiment Ⅳ group.

Except for the white rabbits in the normal control group without any treatment for modeling, the white rabbits in the other 7 groups were coated with coal tar once a day in the range of 2 cm × 2 cm at the opening of the inner ear canal of the left and right ears of each white rabbit. Apply 0.25ml. 2 weeks in a row. at 14d. Two model-making white rabbits were randomly selected for skin biopsy, which confirmed that the model was successfully established.

Medication adopts external administration method. Apply normal saline 0.1ml, 0.1% vitamin C to the model-making parts of the left and right ears of white rabbits in the model control group, positive drug control group, Coptidis extract group, experimental group I, experimental group II, experimental group III and experimental group IV respectively. A acid cream 0.1g, experimental group I preparation 0.1ml, experimental group II 0.1ml, experimental group III 0.1ml and experimental group IV 0.1ml, 2 times a day for 2 consecutive weeks. The normal control group still did not receive any treatment.

24 hours after the last drug administration, a 3mm hole punch was used to punch a hole in the drug-applied rabbit ear to make a specimen of the ear piece (full-thickness skin). After being fixed with 10% formalin and sent to pathology, after HE staining, the histopathological changes were observed under light microscope. According to the thickening of the epidermis, the expansion degree of the hair follicle opening and the amount of keratin, the pathological changes of the specimen were divided into 4 grades: "0" means no acne; "+" means a small amount of dense keratin in the infundibulum of the hair follicle; A moderate amount of keratinization can be seen in the infundibulum, extending toward the sebaceous gland; "+++" means extensive keratinization in the expanded hair follicle.

Statistical method In this experiment, after grading the pathological and histological changes of the experimental specimens in each group, the rank sum test was used to compare the differences among the groups.

After administration, the rabbit ears of each group were observed with naked eyes

Normal control group: the rabbit ears are thin and soft. Clear capillaries can be seen. The hair follicles can be seen in neat arrangement at the opening of the ear canal of the rabbit ears. Uniform size. Model control group: 1 week after applying coal tar. It can be seen that the thickness of the ears in the modeling area increases and becomes hard, and there are black horn plugs at the hair follicle mouth. After 2 weeks of coal tar application, it can be seen that the hair follicle mouth of the modeling site has obvious horn plugs, and the hair follicle mouth is raised in the form of blackheads and papules, and the surface of the entire rabbit ear is rough. After another 2 weeks of external application of physiological saline, obvious hair follicle angle plugs can still be seen in the ears of the modeling site.

Positive drug control group and experimental group Ⅰ after 2 weeks of treatment. The thickening of the ears is alleviated to varying degrees, and the local hair follicle horn plugs are reduced; after some horn plugs fall off, there are only a few punctate depressed hair follicles, and the papules become flat and reduced. Most of the horn plugs in experimental group III and experimental group IV fell off, but there were many point-shaped depressed hair follicles left at the position of the falling off. Obvious hair follicle angle plugs can still be seen in the Coptis chinensis extract group and the experimental group Ⅱ.

Histological observation of rabbits in each group after administration

Normal control group: the epidermis is thinner, the junction between epidermis and dermis is clear, and hair follicles can be seen. Model control group: irregular hyperplasia and hyperkeratosis of epidermis and hair follicle epithelium can be seen. Pores are dilated and filled with keratinized substances. Adjacent hair follicles fuse with each other, and the infundibulum of the hair follicle expands like a pot.

In the experimental group Ⅰ, after 2 weeks of treatment, the thickening of the epidermis was eliminated, and there was no expansion of the hair follicle orifice. Positive drug control group, experimental group Ⅲ and experimental group Ⅳ: epidermis thickening alleviated to varying degrees, no hair follicle orifice dilated or some hair follicle orifices slightly dilated; a very small amount to a small amount of loose keratinized material filled, no hair follicle infundibulum enlarged such as Pot-shaped. In the Coptidis extract group, the expansion of hair follicles and the filling degree of keratinized substances in the experimental group II were slightly reduced, but not obvious.

The grading results of histopathological examination are shown in Table 2.

Table 2 Effects of different emulsifiers of Coptis rhizome external preparations on rabbit ear experimental keratosis model

Note: Compared with the model group, ^* P﹤0.05, ^** P﹤0.01; ^ΔP ﹥0.05.

From the above data, it can be known that the combination of Coptidis Rhizome extract, lecithin and tannic acid can effectively eliminate the problem of hyperkeratosis of skin pilosebaceous gland ducts. However, the combination of Coptidis rhizome extract, lauric monoglyceride and tannic acid and the combination of Coptidis rhizome extract and tannic acid did not significantly improve the problem of keratosis. The combination of Coptis chinensis extract, Tween-80 and tannic acid does not have the effect of eliminating keratinization. The extract of Coptidis Rhizoma also does not have the effect of eliminating keratinization.

The effect of embodiment 4 coptis external preparations on eczema

sample preparation

Crush Coptidis medicinal material, sieve, divide into 3 groups, each group has 3 parts, each part is 100g; extract each part of Coptidis medicinal material with 5 times, 3 times and 3 times of water reflux, each time for 30min, cool, remove impurities, concentrate to 30g of raw medicinal materials/100ml, let it stand for cooling, filter the precipitate to obtain the water extract of Coptis chinensis; add ethanol to the water extract of Coptis chinensis, adjust the alcohol concentration to 50-60%, let it stand and filter the precipitate to obtain the clarified solution of Coptis chinensis; recover the ethanol and add water and the ethanol solution to adjust the ethanol concentration of the solvent to 2-5%, and each 100ml contains 30g of Coptis chinensis original medicinal material extract to obtain Coptis chinensis extract. Heat each portion of Coptis chinensis extract to 50°C, add chitosan clarifier to each portion according to 5% of the volume of the liquid medicine, stir at 100r·min ^-1 for 10min while adding, let the solution cool down and let it stand for 12h ^, 10000r·min -1 ^1. Centrifuge, filter, and wave off acetic acid to obtain chitosan treatment liquid.

Get wherein one group of chitosan treatment liquids add water, ethanol tannic acid and soybean lecithin, make every 100ml contain the extract of 15g Coptidis rhizome former medicinal material, 0.6g tannic acid and 1.2g soybean lecithin, the concentration of solvent ethanol is 2- 5%; when the time comes for the experimental group V.

Get wherein one group of chitosan treatment liquids add water, ethanol tannic acid and soybean lecithin, make every 100ml contain the extract of 30g Coptidis rhizome former medicinal material, 0.3g tannic acid and 0.7g soybean lecithin, the concentration of solvent ethanol is 2- 5%; when the experimental group VI.

Prepare a group containing 0.5g tannic acid and 0.9g soybean lecithin per 100ml, not containing Coptidis rhizome extract, and the concentration of solvent ethanol is 2-5%; obtain matrix control group.

Effect on eczema

Effects of p-xylene on mouse auricle swelling

Seventy male mice were randomly divided into model control group, matrix control group, dexamethasone acetate cream group, Coptis chinensis extract group, experimental group I, experimental group V and experimental group VI, with 10 mice in each group. In addition to the model control group, each group used petroleum jelly, dexamethasone acetate cream, Coptis chinensis extract group, experimental group I, experimental group V and experimental group VI to prepare the liquid, and evenly applied to the left and right auricles of the mice, 2.5 g/kg, once a day for 4 consecutive days. One hour after the last application, wash off the drug with distilled water and wipe it off with a dry cotton ball. Drop 0.04ml of xylene on the auricle of the right ear of the mouse, dislocate and kill the mouse after 1.5h, use a 9mm puncher to remove the two ear slices from the same part of each mouse, weigh them, and calculate the difference between the two ear slices The value is the swelling degree of the auricle.

Effects on Dextran 40-induced Scratch Response in Mice

70 mice, half male and half male, were first depilated on the back of the neck at 2cm×2cm, and randomly divided into model control group, matrix control group, dexamethasone acetate cream group, Coptidis extract group, experimental group I, and experimental group as above. In group V and group VI, each group used vaseline, dexamethasone acetate cream, Coptis chinensis extract group, group I, group V and group VI, and applied them evenly to the left and right auricles of the mice. 2.5g/kg, once a day, for 4 consecutive days. One hour after the last administration, each mouse was injected with 0.025% low molecular weight dextran 5ml/kg (1.25mg/kg) through the tail vein. After injection, the scratching latency of each mouse and the number of scratching times of mice in each group within 30 min were observed and recorded.

Experimental Study on Treatment of Chronic Eczema with Coptidis External Preparation

Seventy guinea pigs, half male and half male, were randomly divided into model control group, matrix control group, dexamethasone acetate cream group, Coptis chinensis extract group, experimental group I, experimental group V and experimental group VI. Each guinea pig’s nape was cut to a size of 2cm×2cm, and sensitized with 25l of 5% DNCB acetone solution. After 2 weeks, 100l of 0.1% DNCB acetone solution was applied to the inner side of the right ear of the guinea pig for stimulation once a week for 4 consecutive weeks. At 72 hours after the fourth challenge, except for the model control group, each group applied vaseline, dexamethasone acetate cream, high-dose, medium-dose, and low-dose ointment of glycyrrhizinic acid, all of which were applied to the two auricles of the mice, 2.5g/ kg, once a day for 10 consecutive days. After 10 days of administration, stop the drug for 1 day, measure the thickness of the middle part of the left and right ears of each guinea pig with a vernier caliper, and calculate the difference, which is the degree of swelling. The guinea pigs were sacrificed, and the right ear skin tissue was taken 10mm×2mm, fixed in 4% formaldehyde, embedded in paraffin, sectioned, stained with hematoxylin-eosin (HE), and the pathological changes of the ear skin were observed under a light microscope.

Statistical method for the above-mentioned experimental group: using SPSS15.0 statistical software package, using one-way analysis of variance for statistical analysis, P<0.05 means the difference is statistically significant. See Table 3 for details.

Table 3 Effects of external preparations of Coptidis rhizome on mouse auricle swelling and scratching response and guinea pig auricle swelling

Note: Compared with the model group, ^** P﹤0.05; ^* P﹤0.01.

The above experimental data showed that in the investigation of the effect of the external preparation of Coptidis rhizome on the swelling of the auricles of mice induced by xylene, the swelling of the auricles of the mice in the model control group was obvious 1.5 hours after the model was established. The dexamethasone acetate cream group, experimental group Ⅰ, experimental group Ⅴ and experimental group Ⅵ significantly reduced the ear swelling, compared with the model control group, the difference was statistically significant (P<0.05 or <0.01). Coptis chinensis extract group and matrix control group can also reduce auricle swelling (P<0.05).

In the investigation of the effect of Coptis rhizome external preparations on the scratching response of mice induced by dextran 40, the mice in the model control group had pruritus symptoms after modeling, which manifested as frequent scratching. The dexamethasone acetate cream group, experimental group Ⅰ, experimental group Ⅴ and experimental group Ⅵ significantly prolonged the scratching latency, and the dexamethasone acetate cream group, matrix control, experimental group Ⅰ, and experimental group Ⅴ had significant scratching times within 30 minutes. Compared with the model control group, the difference was statistically significant (P<0.05). Coptidis extract group and experimental group Ⅵ can also reduce the number of scratching within 30 minutes.

In the experimental study on the treatment of chronic eczema with external preparations of Coptis chinensis, the dexamethasone acetate cream group, experimental group Ⅰ, experimental group Ⅴ and experimental group Ⅵ could significantly inhibit the ear swelling of guinea pigs with chronic eczema. Compared with the model control group, the difference Both were statistically significant (P<0.01). Coptidis extract group and matrix control group also can (P<0.05).

Claims (5)

1. A coptis external preparation, characterized in that: said external preparation is composed of Coptidis extract, astringent and surfactant; said astringent is tannic acid, and surfactant is composed of soybean lecithin; said Coptidis extract The weight ratio of the original medicinal material, tannic acid and soybean lecithin of the product is 15-30:0.3-0.6:0.7-1.2; The preparation method of described coptis external preparation, comprises the following steps: 1) Pulverize Coptidis Rhizoma medicinal material, sieve; 2) extracting Coptis chinensis medicinal material with water reflux, cooling, removing impurities, concentrating to 30g of the original medicinal material/100ml, standing for cooling, filtering the precipitate, and obtaining Coptis chinensis water extract; 3) Coptis chinensis extract is clarified twice with UTA-specific chitosan; 4) adding ethanol to the water extract of Coptidis rhizome, adjusting the alcohol concentration to 50-60%, standing and filtering the precipitate to obtain the clarified solution of Coptidis rhizome; 5) Recover ethanol and add water and ethanol solution to adjust the solvent ethanol concentration to 2-5%, and each 100ml contains 15-20g of Coptis chinensis original medicinal material extract to obtain Coptis chinensis extract; 6) adding tannic acid and soybean lecithin to prepare a Coptis rhizome external preparation. 2. The external preparation of Coptis chinensis according to claim 1, characterized in that: the weight ratio of the original medicinal material of Coptis chinensis, tannic acid and soybean lecithin in the external preparation is 20:0.5:0.9. 3. The external preparation of Coptis chinensis according to claim 2, characterized in that: every 100ml of said external preparation contains Coptis chinensis extract weighing 20g of the original medicinal material, 0.5g of tannic acid and 0.9g of soybean lecithin; The solvent of the external preparation is 2-5% ethanol; it is made into one of liniment, spray or aerosol. 4. The application of the Coptis rhizome external preparation described in any one of claims 1-3 in the preparation of medicine for treating eczema. 5. The application of the Coptis rhizome external preparation described in any one of claims 1-3 in the preparation of a medicament for treating acne.