CN104257707A - Traditional Chinese medicine extract for treating tympanitis and preparation method of traditional Chinese medicine extract - Google Patents
Traditional Chinese medicine extract for treating tympanitis and preparation method of traditional Chinese medicine extract Download PDFInfo
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- 239000000284 extract Substances 0.000 title claims abstract description 110
- 239000003814 drug Substances 0.000 title claims abstract description 79
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 208000025301 tympanitis Diseases 0.000 title abstract 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 33
- 239000002024 ethyl acetate extract Substances 0.000 claims abstract description 27
- 238000003815 supercritical carbon dioxide extraction Methods 0.000 claims abstract description 14
- 238000002481 ethanol extraction Methods 0.000 claims description 53
- 206010033078 Otitis media Diseases 0.000 claims description 51
- 241001619461 Poria <basidiomycete fungus> Species 0.000 claims description 45
- 238000000605 extraction Methods 0.000 claims description 38
- 241001643642 Viticis Species 0.000 claims description 29
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 27
- 239000007788 liquid Substances 0.000 claims description 21
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 18
- 229940079593 drug Drugs 0.000 claims description 18
- 239000000706 filtrate Substances 0.000 claims description 18
- 238000000227 grinding Methods 0.000 claims description 18
- 238000010298 pulverizing process Methods 0.000 claims description 18
- 235000015221 Bupleurum rotundifolium Nutrition 0.000 claims description 9
- 244000236521 Bupleurum rotundifolium Species 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 9
- 238000004108 freeze drying Methods 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 9
- 238000000926 separation method Methods 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 9
- 229960004756 ethanol Drugs 0.000 claims description 7
- 238000001291 vacuum drying Methods 0.000 claims description 7
- 239000012141 concentrate Substances 0.000 claims description 2
- 208000024891 symptom Diseases 0.000 abstract description 18
- 208000009205 Tinnitus Diseases 0.000 abstract description 9
- 230000000903 blocking effect Effects 0.000 abstract description 9
- 231100000886 tinnitus Toxicity 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 2
- 238000000638 solvent extraction Methods 0.000 abstract description 2
- 229940126680 traditional chinese medicines Drugs 0.000 abstract 2
- UFLHIIWVXFIJGU-ARJAWSKDSA-N (Z)-hex-3-en-1-ol Chemical compound CC\C=C/CCO UFLHIIWVXFIJGU-ARJAWSKDSA-N 0.000 abstract 1
- 206010011878 Deafness Diseases 0.000 abstract 1
- 244000235756 Microcos paniculata Species 0.000 abstract 1
- 244000197580 Poria cocos Species 0.000 abstract 1
- 235000008599 Poria cocos Nutrition 0.000 abstract 1
- 244000248021 Vitex negundo Species 0.000 abstract 1
- 235000013427 Vitex negundo var incisa Nutrition 0.000 abstract 1
- 235000014961 Vitex negundo var negundo Nutrition 0.000 abstract 1
- 235000013399 edible fruits Nutrition 0.000 abstract 1
- 230000010370 hearing loss Effects 0.000 abstract 1
- 231100000888 hearing loss Toxicity 0.000 abstract 1
- 208000016354 hearing loss disease Diseases 0.000 abstract 1
- 235000014956 negundo chastetree Nutrition 0.000 abstract 1
- 208000035824 paresthesia Diseases 0.000 description 10
- 206010013886 Dysaesthesia Diseases 0.000 description 9
- 230000003203 everyday effect Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 229940126678 chinese medicines Drugs 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 206010014020 Ear pain Diseases 0.000 description 1
- 208000005141 Otitis Diseases 0.000 description 1
- 241000239292 Theraphosidae Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000009670 cang er zi wan Substances 0.000 description 1
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 1
- 229960003957 dexamethasone Drugs 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 208000019258 ear infection Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- UAJTZZNRJCKXJN-UHFFFAOYSA-M sodium;2-dodecoxy-2-oxoethanesulfonate Chemical compound [Na+].CCCCCCCCCCCCOC(=O)CS([O-])(=O)=O UAJTZZNRJCKXJN-UHFFFAOYSA-M 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/24—Apocynaceae (Dogbane family), e.g. plumeria or periwinkle
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/233—Bupleurum
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- A61K2236/30—Extraction of the material
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- A61K2236/30—Extraction of the material
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- A61K2236/50—Methods involving additional extraction steps
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Abstract
The invention belongs to the field of traditional Chinese medicines and discloses a traditional Chinese medicine extract for treating tympanitis and a preparation method of the traditional Chinese medicine extract. The traditional Chinese medicine extract is prepared from the following components in parts by weight: 8-15 parts of paniculate microcos leaf alcohol extract, 6-10 parts of radix bupleuri alcohol extract, 3-8 parts of poria cocos ethyl acetate extract and 3-5 parts of negundo chastetree fruit supercritical extract. The preparation method of the traditional Chinese medicine extract comprises the following steps: extracting different traditional Chinese medicines by virtue of different methods such solvent extraction, and supercritical carbon dioxide extraction; mixing the extracts to obtain the traditional Chinese medicine extract for treating tympanitis. The traditional Chinese medicine extract obtained by the preparation method can be used for effectively eliminating or relieving symptoms such as tinnitus, aural fullness, blocking and hearing loss.
Description
Technical field
The invention belongs to tcm field, relate to a kind of Chinese medicine and preparation method thereof, particularly relate to a kind of Chinese medicine extract for the treatment of otitis media and preparation method thereof.
Background technology
Otitis media mainly with hydrotympanum and auditory dysesthesia for principal character, the initial stage is main mainly with bulge of the ear, and the later stage has the symptoms such as ear pain.In addition because otitis media morbidity is very fast, more than with symptoms such as auditory dysesthesias.TCM investigation is thought, the cause of disease of otitis media mostly is the functional disorders such as liver, spleen, lung, caused by rheumatism cold and heat etc. are piled up in vivo.
Otitis media can be divided into affection due to external wind and heat, affection of exogenous wind-cold etc. according to symptom.The Chinese medicine for the treatment of otitis media is more, the Chinese medicines such as such as Herba Plantaginis, Rhizoma Alismatis, Radix Saposhnikoviae, Fructus Xanthii, Radix Puerariae, Radix Salviae Miltiorrhizae.The patent No. be the disclosure of the invention of 101978968 treatment otitis media Chinese medicine be tarantula and Alumen.What it was too much takes the certain side effect of rear generation.The Western medicine aspect of otitis in the treatment in addition, dexamethasone has good curative effect.Though the Chinese medicine for the treatment of otitis media is many, but its ratio of curing otitis media is completely not high, the clinical research about otitis media still has a lot of problem to need to solve.Need us to probe into mechanism and the pathology of healing otitis media further, filter out more effective Chinese medicine formula.
Summary of the invention
The technical problem solved: use Chinese medicine all poor to the method effect for the treatment of otitis media at present, tinnitus, the ear that can not slow down patient are preferably vexed, blocking, auditory dysesthesia symptom, the problem that the otitis media that result in patient can not effectively be treated, therefore needs a kind of Chinese medicine extract for the treatment of otitis media and preparation method thereof.
Technical scheme: for the problems referred to above, the invention discloses a kind of Chinese medicine extract for the treatment of otitis media and preparation method thereof.The Chinese medicine extract of described treatment otitis media comprises the extract of following weight parts: Folium Microcoris paniculatae ethanol extraction is 8 ~ 15 parts, Radix Bupleuri ethanol extraction is 6 ~ 10 parts, Poria ethyl acetate extract is 3 ~ 8 parts.
Preferably, described a kind of Chinese medicine extract for the treatment of otitis media, comprises the extract of following weight parts: Folium Microcoris paniculatae ethanol extraction is 8 ~ 15 parts, Radix Bupleuri ethanol extraction is 6 ~ 10 parts, Poria ethyl acetate extract is 3 ~ 8 parts, Fructus Viticis Negundo supercritical extract is 3 ~ 5 parts.
Preferred further, described a kind of Chinese medicine extract for the treatment of otitis media, Chinese medicine extract comprises the extract of following weight parts: Folium Microcoris paniculatae ethanol extraction is 12 parts, Radix Bupleuri ethanol extraction is 9 parts, Poria ethyl acetate extract is 6 parts, Fructus Viticis Negundo supercritical extract is 4 parts.
Described a kind of preparation method for the treatment of the Chinese medicine extract of otitis media, preparation method comprises the following steps:
(1) pulverize after Folium Microcoris paniculatae lyophilization, extract with dehydrated alcohol after pulverizing, filter after extracting, filtrate is carried out after vacuum film concentrates, obtain Folium Microcoris paniculatae ethanol extraction; (2) Radix Bupleuri is carried out baking the affected part after applying some drugs to do, grinding after baking the affected part after applying some drugs is dry, extracts Chinese Thorowax Root dehydrated alcohol after grinding becomes powder, filters after extracting, and prepares Radix Bupleuri ethanol extraction after filtrate is concentrated; (3) Poria is dried, after drying, Poria is pulverized, extract by ethyl acetate after pulverizing, be separated after extracting, after separation, supernatant concentration obtained Poria ethyl acetate extract; (4) carry out supercritical carbon dioxide extraction by after Fructus Viticis Negundo vacuum drying, collect separating still after extraction, gleanings is Fructus Viticis Negundo supercritical extract; (5) get that Folium Microcoris paniculatae ethanol extraction is 8 ~ 15 parts, Radix Bupleuri ethanol extraction is 6 ~ 10 parts, Poria ethyl acetate extract is 3 ~ 8 parts, Fructus Viticis Negundo supercritical extract is 3 ~ 5 parts by weight, said extracted thing is mixed, for treating the Chinese medicine extract of otitis media.
Preferably, described a kind of preparation method for the treatment of the Chinese medicine extract of otitis media, comprise the following steps: (1) pulverizes after Folium Microcoris paniculatae lyophilization, extract with dehydrated alcohol after pulverizing, Extracting temperature is 50 DEG C, and extraction time is 2h, and solid-liquid ratio is 1:6, extract after filter, filtrate is carried out vacuum film be concentrated into without ethanol outflow after obtain Folium Microcoris paniculatae ethanol extraction; (2) Radix Bupleuri is carried out baking the affected part after applying some drugs to do, hot bake out temperature is 85 DEG C, grinding after baking the affected part after applying some drugs is dry, it is extracted by Chinese Thorowax Root dehydrated alcohol after 300 object powder that grinding becomes order number, Extracting temperature is 60 DEG C, and extraction time is 1h, and solid-liquid ratio is 1:5, filter after extracting, after filtrate is concentrated, prepare Radix Bupleuri ethanol extraction; (3) Poria is dried, after drying, Poria is pulverized, extract by ethyl acetate after pulverizing, Extracting temperature is 40 DEG C, and extraction time is 4h, and solid-liquid ratio is 1:4, centrifugalize after extracting, centrifugal rotational speed is 5000rpm, after separation, supernatant concentration is obtained Poria ethyl acetate extract; (4) carry out supercritical carbon dioxide extraction by after Fructus Viticis Negundo vacuum drying, supercritical carbon dioxide extraction pressure is 20MPa, and separating still pressure is 6MPa, extraction temperature is 50 DEG C, extraction time is 2h, collects separating still after extraction, and gleanings is Fructus Viticis Negundo supercritical extract; (5) get that Folium Microcoris paniculatae ethanol extraction is 8 ~ 15 parts, Radix Bupleuri ethanol extraction is 6 ~ 10 parts, Poria ethyl acetate extract is 3 ~ 8 parts, Fructus Viticis Negundo supercritical extract is 3 ~ 5 parts by weight, said extracted thing is mixed, for treating the Chinese medicine extract of otitis media.
In the Chinese medicine extract that above-mentioned treatment otitis media is large, independent Chinese medicine such as Folium Microcoris paniculatae, Radix Bupleuri, Poria, Fructus Viticis Negundo may not possess the effect for the treatment of otitis media, but after composition effective in above-mentioned four kinds of Chinese medicines is carried out solvent extraction or supercritical carbon dioxide extraction, mixed Chinese medicine extract achieves the good result for the treatment of otitis media.
Beneficial effect: of the present inventionly get that Folium Microcoris paniculatae ethanol extraction is 8 ~ 15 parts, Radix Bupleuri ethanol extraction is 6 ~ 10 parts, Poria ethyl acetate extract is 3 ~ 8 parts, Fructus Viticis Negundo supercritical extract is 3 ~ 5 parts by weight, especially when Folium Microcoris paniculatae ethanol extraction be 12 parts, Radix Bupleuri ethanol extraction is 9 parts, Poria ethyl acetate extract is 6 parts, Fructus Viticis Negundo supercritical extract is 4 parts time, the tinnitus that the treatment otitis media of Chinese medicine extract causes, ear are vexed, blocking, auditory dysesthesia symptom obtain and remove completely or alleviate, effectively can cure otitis media.
Detailed description of the invention
Embodiment 1
(1) pulverize after Folium Microcoris paniculatae lyophilization, extract after pulverizing with dehydrated alcohol, Extracting temperature is 50 DEG C, extraction time is 2h, solid-liquid ratio is 1:6, extract after filter, filtrate is carried out vacuum film be concentrated into without ethanol outflow after obtain Folium Microcoris paniculatae ethanol extraction; (2) Radix Bupleuri is carried out baking the affected part after applying some drugs to do, hot bake out temperature is 85 DEG C, grinding after baking the affected part after applying some drugs is dry, it is extracted by Chinese Thorowax Root dehydrated alcohol after 300 object powder that grinding becomes order number, Extracting temperature is 60 DEG C, and extraction time is 1h, and solid-liquid ratio is 1:5, filter after extracting, after filtrate is concentrated, prepare Radix Bupleuri ethanol extraction; (3) Poria is dried, after drying, Poria is pulverized, extract by ethyl acetate after pulverizing, Extracting temperature is 40 DEG C, and extraction time is 4h, and solid-liquid ratio is 1:4, centrifugalize after extracting, centrifugal rotational speed is 5000rpm, after separation, supernatant concentration is obtained Poria ethyl acetate extract; (4) carry out supercritical carbon dioxide extraction by after Fructus Viticis Negundo vacuum drying, supercritical carbon dioxide extraction pressure is 20MPa, and separating still pressure is 6MPa, extraction temperature is 50 DEG C, extraction time is 2h, collects separating still after extraction, and gleanings is Fructus Viticis Negundo supercritical extract; (5) getting that Folium Microcoris paniculatae ethanol extraction is 12 parts, Radix Bupleuri ethanol extraction is 9 parts, Poria ethyl acetate extract is 6 parts, Fructus Viticis Negundo supercritical extract is 4 parts by weight, mixed by said extracted thing, is the Chinese medicine extract for the treatment of otitis media.
10 patients take the Chinese medicine extract of embodiment 1, every day, taking dose was 20g, and once a day, the cycle of taking is 10 days, the tinnitus of 9 patients in 10 patients after 10 days, ear are vexed, blocking, auditory dysesthesia symptom eliminated completely, the symptom of 1 patient is effectively alleviated.
Embodiment 2
(1) pulverize after Folium Microcoris paniculatae lyophilization, extract after pulverizing with dehydrated alcohol, Extracting temperature is 50 DEG C, extraction time is 2h, solid-liquid ratio is 1:6, extract after filter, filtrate is carried out vacuum film be concentrated into without ethanol outflow after obtain Folium Microcoris paniculatae ethanol extraction; (2) Radix Bupleuri is carried out baking the affected part after applying some drugs to do, hot bake out temperature is 85 DEG C, grinding after baking the affected part after applying some drugs is dry, it is extracted by Chinese Thorowax Root dehydrated alcohol after 300 object powder that grinding becomes order number, Extracting temperature is 60 DEG C, and extraction time is 1h, and solid-liquid ratio is 1:5, filter after extracting, after filtrate is concentrated, prepare Radix Bupleuri ethanol extraction; (3) Poria is dried, after drying, Poria is pulverized, extract by ethyl acetate after pulverizing, Extracting temperature is 40 DEG C, and extraction time is 4h, and solid-liquid ratio is 1:4, centrifugalize after extracting, centrifugal rotational speed is 5000rpm, after separation, supernatant concentration is obtained Poria ethyl acetate extract; (4) carry out supercritical carbon dioxide extraction by after Fructus Viticis Negundo vacuum drying, supercritical carbon dioxide extraction pressure is 20MPa, and separating still pressure is 6MPa, extraction temperature is 50 DEG C, extraction time is 2h, collects separating still after extraction, and gleanings is Fructus Viticis Negundo supercritical extract; (5) getting that Folium Microcoris paniculatae ethanol extraction is 8 parts, Radix Bupleuri ethanol extraction is 10 parts, Poria ethyl acetate extract is 8 parts, Fructus Viticis Negundo supercritical extract is 5 parts by weight, mixed by said extracted thing, is the Chinese medicine extract for the treatment of otitis media.
10 patients take the Chinese medicine extract of embodiment 2, every day, taking dose was 20g, and once a day, the cycle of taking is 10 days, the tinnitus of 6 patients in 10 patients after 10 days, ear are vexed, blocking, auditory dysesthesia symptom eliminated completely, the symptom of 4 patients is effectively alleviated.
Embodiment 3
(1) pulverize after Folium Microcoris paniculatae lyophilization, extract after pulverizing with dehydrated alcohol, Extracting temperature is 50 DEG C, extraction time is 2h, solid-liquid ratio is 1:6, extract after filter, filtrate is carried out vacuum film be concentrated into without ethanol outflow after obtain Folium Microcoris paniculatae ethanol extraction; (2) Radix Bupleuri is carried out baking the affected part after applying some drugs to do, hot bake out temperature is 85 DEG C, grinding after baking the affected part after applying some drugs is dry, it is extracted by Chinese Thorowax Root dehydrated alcohol after 300 object powder that grinding becomes order number, Extracting temperature is 60 DEG C, and extraction time is 1h, and solid-liquid ratio is 1:5, filter after extracting, after filtrate is concentrated, prepare Radix Bupleuri ethanol extraction; (3) Poria is dried, after drying, Poria is pulverized, extract by ethyl acetate after pulverizing, Extracting temperature is 40 DEG C, and extraction time is 4h, and solid-liquid ratio is 1:4, centrifugalize after extracting, centrifugal rotational speed is 5000rpm, after separation, supernatant concentration is obtained Poria ethyl acetate extract; (4) carry out supercritical carbon dioxide extraction by after Fructus Viticis Negundo vacuum drying, supercritical carbon dioxide extraction pressure is 20MPa, and separating still pressure is 6MPa, extraction temperature is 50 DEG C, extraction time is 2h, collects separating still after extraction, and gleanings is Fructus Viticis Negundo supercritical extract; (5) getting that Folium Microcoris paniculatae ethanol extraction is 15 parts, Radix Bupleuri ethanol extraction is 6 parts, Poria ethyl acetate extract is 3 parts, Fructus Viticis Negundo supercritical extract is 3 parts by weight, mixed by said extracted thing, is the Chinese medicine extract for the treatment of otitis media.
10 patients take the Chinese medicine extract of embodiment 3, every day, taking dose was 20g, and once a day, the cycle of taking is 10 days, the tinnitus of 7 patients in 10 patients after 10 days, ear are vexed, blocking, auditory dysesthesia symptom eliminated completely, the symptom of 3 patients is effectively alleviated.
Embodiment 4
(1) pulverize after Folium Microcoris paniculatae lyophilization, extract after pulverizing with dehydrated alcohol, Extracting temperature is 50 DEG C, extraction time is 2h, solid-liquid ratio is 1:6, extract after filter, filtrate is carried out vacuum film be concentrated into without ethanol outflow after obtain Folium Microcoris paniculatae ethanol extraction; (2) Radix Bupleuri is carried out baking the affected part after applying some drugs to do, hot bake out temperature is 85 DEG C, grinding after baking the affected part after applying some drugs is dry, it is extracted by Chinese Thorowax Root dehydrated alcohol after 300 object powder that grinding becomes order number, Extracting temperature is 60 DEG C, and extraction time is 1h, and solid-liquid ratio is 1:5, filter after extracting, after filtrate is concentrated, prepare Radix Bupleuri ethanol extraction; (3) Poria is dried, after drying, Poria is pulverized, extract by ethyl acetate after pulverizing, Extracting temperature is 40 DEG C, and extraction time is 4h, and solid-liquid ratio is 1:4, centrifugalize after extracting, centrifugal rotational speed is 5000rpm, after separation, supernatant concentration is obtained Poria ethyl acetate extract; (4) getting that Folium Microcoris paniculatae ethanol extraction is 15 parts, Radix Bupleuri ethanol extraction is 6 parts, Poria ethyl acetate extract is 3 parts by weight, mixed by said extracted thing, is the Chinese medicine extract for the treatment of otitis media.
10 patients take the Chinese medicine extract of embodiment 4, every day, taking dose was 20g, and once a day, the cycle of taking is 10 days, the tinnitus of 3 patients in 10 patients after 10 days, ear are vexed, blocking, auditory dysesthesia symptom eliminated completely, the symptom of 7 patients is effectively alleviated.
Embodiment 5
(1) pulverize after Folium Microcoris paniculatae lyophilization, extract after pulverizing with dehydrated alcohol, Extracting temperature is 50 DEG C, extraction time is 2h, solid-liquid ratio is 1:6, extract after filter, filtrate is carried out vacuum film be concentrated into without ethanol outflow after obtain Folium Microcoris paniculatae ethanol extraction; (2) Radix Bupleuri is carried out baking the affected part after applying some drugs to do, hot bake out temperature is 85 DEG C, grinding after baking the affected part after applying some drugs is dry, it is extracted by Chinese Thorowax Root dehydrated alcohol after 300 object powder that grinding becomes order number, Extracting temperature is 60 DEG C, and extraction time is 1h, and solid-liquid ratio is 1:5, filter after extracting, after filtrate is concentrated, prepare Radix Bupleuri ethanol extraction; (3) Poria is dried, after drying, Poria is pulverized, extract by ethyl acetate after pulverizing, Extracting temperature is 40 DEG C, and extraction time is 4h, and solid-liquid ratio is 1:4, centrifugalize after extracting, centrifugal rotational speed is 5000rpm, after separation, supernatant concentration is obtained Poria ethyl acetate extract; (4) getting that Folium Microcoris paniculatae ethanol extraction is 8 parts, Radix Bupleuri ethanol extraction is 10 parts, Poria ethyl acetate extract is 8 parts by weight, mixed by said extracted thing, is the Chinese medicine extract for the treatment of otitis media.
10 patients take the Chinese medicine extract of embodiment 5, every day, taking dose was 20g, and once a day, the cycle of taking is 10 days, the tinnitus of 4 patients in 10 patients after 10 days, ear are vexed, blocking, auditory dysesthesia symptom eliminated completely, the symptom of 6 patients is effectively alleviated.
Contrast experiment
Get that Fructus Forsythiae is 3 parts, Radix Scutellariae is 6 parts, Herba Houttuyniae is 7 parts, Herba Violae is 2 parts, Radix Bupleuri is 8 parts, Radix Glycyrrhizae is 4 parts, decoct relief patient and take.Every day, taking dose was 20g, and once a day, the cycle of taking is 10 days, the tinnitus of 2 patients in 10 patients after 10 days, ear are vexed, blocking, auditory dysesthesia symptom eliminated completely, the symptom of 8 patients is effectively alleviated.
Claims (10)
1. treat a Chinese medicine extract for otitis media, it is characterized in that the Chinese medicine extract of described treatment otitis media comprises the extract of following weight parts: Folium Microcoris paniculatae ethanol extraction is 8 ~ 15 parts, Radix Bupleuri ethanol extraction is 6 ~ 10 parts, Poria ethyl acetate extract is 3 ~ 8 parts.
2. a kind of Chinese medicine extract for the treatment of otitis media according to claim 1, is characterized in that the Chinese medicine extract of described treatment otitis media comprises the extract of following weight parts: Folium Microcoris paniculatae ethanol extraction is 8 ~ 15 parts, Radix Bupleuri ethanol extraction is 6 ~ 10 parts, Poria ethyl acetate extract is 3 ~ 8 parts, Fructus Viticis Negundo supercritical extract is 3 ~ 5 parts.
3. a kind of Chinese medicine extract for the treatment of otitis media according to claim 2, is characterized in that in the Chinese medicine extract of described treatment otitis media, Folium Microcoris paniculatae ethanol extraction is 12 parts.
4. a kind of Chinese medicine extract for the treatment of otitis media according to claim 2, is characterized in that in the Chinese medicine extract of described treatment otitis media, Radix Bupleuri ethanol extraction is 9 parts.
5. a kind of Chinese medicine extract for the treatment of otitis media according to claim 2, is characterized in that in the Chinese medicine extract of described treatment otitis media, Poria ethyl acetate extract is 6 parts.
6. a kind of Chinese medicine extract for the treatment of otitis media according to claim 2, is characterized in that in the Chinese medicine extract of described treatment otitis media, Fructus Viticis Negundo supercritical extract is 4 parts.
7. a kind of preparation method for the treatment of the Chinese medicine extract of otitis media according to claim 2, it is characterized in that the preparation method of described Chinese medicine extract comprises the following steps: (1) pulverizes after Folium Microcoris paniculatae lyophilization, extract with dehydrated alcohol after pulverizing, filter after extracting, filtrate is carried out after vacuum film concentrates, obtain Folium Microcoris paniculatae ethanol extraction; (2) Radix Bupleuri is carried out baking the affected part after applying some drugs to do, grinding after baking the affected part after applying some drugs is dry, extracts Chinese Thorowax Root dehydrated alcohol after grinding becomes powder, filters after extracting, and prepares Radix Bupleuri ethanol extraction after filtrate is concentrated; (3) Poria is dried, after drying, Poria is pulverized, extract by ethyl acetate after pulverizing, be separated after extracting, after separation, supernatant concentration obtained Poria ethyl acetate extract; (4) carry out supercritical carbon dioxide extraction by after Fructus Viticis Negundo vacuum drying, collect separating still after extraction, gleanings is Fructus Viticis Negundo supercritical extract; (5) get that Folium Microcoris paniculatae ethanol extraction is 8 ~ 15 parts, Radix Bupleuri ethanol extraction is 6 ~ 10 parts, Poria ethyl acetate extract is 3 ~ 8 parts, Fructus Viticis Negundo supercritical extract is 3 ~ 5 parts by weight, said extracted thing is mixed, for treating the Chinese medicine extract of otitis media.
8. a kind of preparation method for the treatment of the Chinese medicine extract of otitis media according to claim 7, it is characterized in that the preparation method of described Chinese medicine extract comprises the following steps: (1) pulverizes after Folium Microcoris paniculatae lyophilization, extract with dehydrated alcohol after pulverizing, Extracting temperature is 50 DEG C, extraction time is 2h, solid-liquid ratio is 1:6, extract after filter, filtrate is carried out vacuum film be concentrated into without ethanol outflow after obtain Folium Microcoris paniculatae ethanol extraction; (2) Radix Bupleuri is carried out baking the affected part after applying some drugs to do, hot bake out temperature is 85 DEG C, grinding after baking the affected part after applying some drugs is dry, it is extracted by Chinese Thorowax Root dehydrated alcohol after 300 object powder that grinding becomes order number, Extracting temperature is 60 DEG C, and extraction time is 1h, and solid-liquid ratio is 1:5, filter after extracting, after filtrate is concentrated, prepare Radix Bupleuri ethanol extraction; (3) Poria is dried, after drying, Poria is pulverized, extract by ethyl acetate after pulverizing, Extracting temperature is 40 DEG C, and extraction time is 4h, and solid-liquid ratio is 1:4, centrifugalize after extracting, centrifugal rotational speed is 5000rpm, after separation, supernatant concentration is obtained Poria ethyl acetate extract; (4) carry out supercritical carbon dioxide extraction by after Fructus Viticis Negundo vacuum drying, supercritical carbon dioxide extraction pressure is 20MPa, and separating still pressure is 6MPa, extraction temperature is 50 DEG C, extraction time is 2h, collects separating still after extraction, and gleanings is Fructus Viticis Negundo supercritical extract; (5) get that Folium Microcoris paniculatae ethanol extraction is 8 ~ 15 parts, Radix Bupleuri ethanol extraction is 6 ~ 10 parts, Poria ethyl acetate extract is 3 ~ 8 parts, Fructus Viticis Negundo supercritical extract is 3 ~ 5 parts by weight, said extracted thing is mixed, for treating the Chinese medicine extract of otitis media.
9. a kind of preparation method for the treatment of the Chinese medicine extract of otitis media according to claim 8, is characterized in that described Folium Microcoris paniculatae ethanol extraction is 12 parts.
10. a kind of preparation method for the treatment of the Chinese medicine extract of otitis media according to claim 8, is characterized in that described Fructus Viticis Negundo supercritical extract is 4 parts.
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Application publication date: 20150107 |