CN104171410B - A kind of manufacture method of cow feed additive - Google Patents

Abstract

What the manufacture method of a kind of cow feed additive described is the manufacture method of a kind of feed additive, the manufacture method of a kind of cow feed additive.First preparation fermented feed solid medium, then sterilizing, cooling, Rhizopus oryzae seed liquor is inoculated in above-mentioned solid medium, 28～30 DEG C of calorstats ferment 10～12 hours, then proceed to inoculate rhodotorula mucilaginosa seed liquor, relay supervention ferment 50～60 hours at 30～32 DEG C of calorstats, get product through pulverizing, pelletize, packaging after then drying in the sun.The cow feed additive of the present invention contains substantial amounts of Rhizopus oryzae and the thalline of rhodotorula mucilaginosa, containing substantial amounts of ergosterol in the cell membrane of both microorganisms, can be converted into vitamin D under ultraviolet irradiates₂, vitamin D₂The absorption of calcium can be promoted, can effectively prevent and treat milch cow calcium deficiency.

Description

A kind of preparation method of dairy cattle feed additive

technical field

What the present invention describes is a kind of preparation method of feed additive, specifically a kind of preparation method of dairy cattle feed additive.

Background technique

Calcium deficiency is a common nutritional and metabolic disease in dairy cows. Extensive management, poor management, lack of attention to the rational mix of feed, severe lack of calcium, phosphorus and other minerals in the feed, or an imbalance in the ratio of calcium and phosphorus will cause insufficient intake of calcium-containing substances by dairy cows. , resulting in calcium deficiency. Calcium deficiency in dairy cows is very harmful to dairy cows. The mild ones like to lie down, and the severe ones are paralyzed, causing huge losses to the dairy farming industry. It is a problem worthy of attention in dairy farming.

Contents of the invention

The purpose of the invention is to provide a method for preparing a feed additive for preventing calcium deficiency in dairy cows.

The purpose of the present invention is achieved like this:

1. Preparation of Rhodotorula gum seed solution

Weigh a certain amount of cornmeal, place it in a triangular flask, add 6 times the mass of water, then add 0.2% of the mass of cornmeal calcium chloride, stir evenly, add α-amylase at a rate of 10-15 units per gram of cornmeal, Keep warm at 90-95°C for 35-45 minutes, cool to 60°C, adjust the pH to 4.0-4.5, add glucoamylase at a rate of 80-120 units per gram of cornmeal, and maintain saccharification at 58°C-64°C for 4-6 The maize saccharification solution was obtained in 1 hour. Add 0.1% urea, 0.2% ammonium sulfate, 0.1% potassium dihydrogen phosphate, and 0.05% magnesium sulfate to the saccharification solution, sterilize at 121°C for 20 minutes, cool to 30°C, and Inoculate a ring of Rhodotorula japonicus on the slant, and culture it for 16-18 hours under the conditions of 28-30° C. and 150 rpm on a rotary shaker to obtain Rhodotorula japonicus seed solution.

2. Preparation of Rhizopus oryzae seed solution

Weigh a certain mass of barley malt, crush it, add 6 times the mass of water, saccharify at 58-62°C for 3-4 hours, filter and sterilize the filtrate at 121°C for 20 minutes, cool to 30°C, and inoculate from the slope Rhizopus oryzae is cultivated for 24-30 hours under the conditions of 28-30 DEG C and 150 rpm on a rotary shaker to obtain Rhizopus oryzae seed liquid.

3. Production of dairy cattle feed additives

First prepare the fermented feed solid medium, mix bran, rice husk, urea, and ammonium sulfate according to the mass ratio of 80:14:4:2, add water, control the water content of the medium at 55-60%, mix evenly, Steam under 0.1MPa pressure for 30-40 minutes, cool to 30°C, inoculate the Rhizopus oryzae seed liquid into the above solid medium at an inoculum amount of 5%, mix evenly, and spread it on a shallow plate, at 28-30°C Ferment in an incubator for 10-12 hours, then continue to inoculate the Rhodotorula gum seed liquid, the inoculum amount is 10%, spread it on a shallow plate after mixing evenly, and continue to ferment in a 30-32°C incubator for 50-60 hours. Then it is dried in the sun and then crushed, granulated and packaged to get the finished product.

The dairy cow feed additive of the present invention contains a large amount of rhizopus oryzae and Rhodotorula japonicus thalline, and the cell membranes of these _two microorganisms contain a large amount of ergosterol, which can be converted into vitamin D2 under ultraviolet irradiation, and vitamin _D2 can Promote the absorption of calcium, which can effectively prevent calcium deficiency in dairy cows. In addition, the dairy cow feed additive of the present invention also contains a large amount of enzymes, including cellulase, amylase, phytate plum, glucoamylase, xylanase, pectinase, lipase, etc., these enzymes with different functions It can decompose cellulose, hemicellulose, carbohydrates, protein and fat in raw materials into easily absorbed monosaccharides, small peptides, amino acids, etc., which can effectively improve the feed utilization rate of dairy cows. The feed additives for dairy cows enter the rumen of dairy cows, and then With the further reproduction of microorganisms, various enzymes will continue to be produced, which will participate in the metabolism together with the enzymes in the dairy cow feed additive itself, effectively improving the digestion ability and production performance of dairy cows. When feeding dairy cows, it is only necessary to add the dairy cow feed additive of the present invention to the concentrated feed of dairy cows in a proportion of 15-20%.

specific implementation plan

The strains used in the present invention are purchased from the China Industrial Microorganism Strain Collection and Management Center, and the numbers are respectively Rhodotorula japonicus CICC31192 and Rhizopus oryzae CICC3005.

1. Preparation of Rhodotorula gum seed solution

Weigh a certain amount of cornmeal, place it in a triangular flask, add 6 times the mass of water, then add 0.2% calcium chloride of cornmeal quality, stir evenly, add α-amylase at a rate of 10 units per gram of cornmeal, and add α-amylase at 90 Keep warm at ℃ for 45 minutes, cool to 60℃, adjust the pH to 4.5, add glucoamylase at a rate of 120 units per gram of cornmeal, maintain 60℃ for 6 hours to obtain corn saccharification solution, add 0.1% corn flour to the saccharification solution Urea, 0.2% ammonium sulfate, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, sterilize at 121°C for 20 minutes, cool to 30°C, inoculate a ring of Rhodotorula japonicus from the slope, shake at 30°C Cultivate under the condition of bed 150rpm for 18 hours to obtain Rhodotorula gluei seed liquid.

2. Preparation of Rhizopus oryzae seed solution

Weigh a certain mass of barley malt, crush it, add 6 times the mass of water, saccharify at 60°C for 4 hours, filter and sterilize the filtrate at 121°C for 20 minutes, cool to 30°C, inoculate Rhizopus oryzae from the slope, Cultivate for 28 hours at 30° C. under the condition of a rotary shaker at 150 rpm to obtain the Rhizopus oryzae seed solution.

3. Production of dairy cattle feed additives

Prepare fermented feed solid medium first, mix bran, rice husk, urea, and ammonium sulfate in a ratio of 80:14:4:2, add water, control the water content of the medium at 60%, mix well, and press 0.1MPa Steam for 40 minutes, cool to 30°C, inoculate the Rhizopus oryzae seed liquid into the above solid medium at an inoculation amount of 5%, spread it on a shallow plate after mixing evenly, and ferment in a 30°C thermostat for 12 hours, then Continue to inoculate the Rhodotorula japonicus seed liquid, the inoculum amount is 10%, spread it on a shallow plate after mixing evenly, continue to ferment in a 30°C incubator for 55 hours, then dry it in the sun, crush it, granulate it, and pack it That is the finished product.

Claims (1)

1. A method for preparing a feed additive for dairy cows, characterized in that it is prepared through the following processes: ⑴Weigh a certain amount of cornmeal, put it in a triangular flask, add 6 times the mass of water, then add 0.2% calcium chloride of the mass of cornmeal, stir evenly, add α-amylase at a ratio of 10 to 15 units per gram of cornmeal , keep warm at 90-95°C for 35-45 minutes, cool to 60°C, adjust the pH to 4.0-4.5, add glucoamylase at a rate of 80-120 units per gram of cornmeal, and maintain 58°C-64°C to saccharify 4- The corn saccharification solution was obtained in 6 hours. Add 0.1% urea, 0.2% ammonium sulfate, 0.1% potassium dihydrogen phosphate, and 0.05% magnesium sulfate to the saccharification solution, sterilize at 121°C for 20 minutes, and cool to 30°C. Inoculate a ring of Rhodotorula japonicus from the slant, and cultivate it for 16-18 hours under the conditions of 28-30°C and 150 rpm on a rotary shaker to obtain the Rhodotorula japonicus seed solution; (2) Weigh a certain quality of barley malt, crush it, add 6 times the quality of water, saccharify at 58-62°C for 3-4 hours, filter and sterilize the filtrate at 121°C for 20 minutes, cool to 30°C, Inoculate Rhizopus oryzae, and cultivate for 24-30 hours under the conditions of 28-30° C. and 150 rpm on a rotary shaker to obtain Rhizopus oryzae seed liquid; (3) Prepare the fermented feed solid medium first, mix bran, rice husk, urea, and ammonium sulfate according to the mass ratio of 80:14:4:2, add water, control the water content of the medium at 55-60%, and mix well , Steam under 0.1MPa pressure for 30-40 minutes, cool to 30°C, inoculate the Rhizopus oryzae seed liquid into the above-mentioned solid medium at an inoculation amount of 5%, mix it evenly, and spread it on a shallow plate. Ferment in an incubator at ℃ for 10-12 hours, then continue to inoculate Rhodotorula gum seed solution with an inoculation amount of 10%, mix well and spread it on a shallow plate again, and continue to ferment in an incubator at 30-32°C for 50-60 hours , and then dried in the sun and then crushed, granulated, and packaged to obtain the finished product.