CN104160994A - Litopenaeus vannamei larvae rearing and breeding method - Google Patents

Abstract

The invention discloses a litopenaeus vannamei larvae rearing and breeding method. The method comprises the steps of larvae rearing and breeding device assembly and sterilization, larvae rearing and breeding water sterilization, larvae putting and feeding, and larvae rearing water quality control. A breeding device adopted in the method is simple in structure, reasonable in arrangement and convenient and easy to operate and use. Automatic control can be achieved in multiple steps, live algae, probiotics and periodic blow down and water change are adopted for water quality control, ecological safety is guaranteed, the design is reasonable, the water environment for larvae rearing is easier to stabilize, and various diseases are prevented. The litopenaeus vannamei larvae rearing and breeding method is simple and efficient.

Description

A kind of method of Litopenaeus vannamei seed cultivation and cultivation

technical field

The invention belongs to the technical field of aquaculture, and relates to a method for cultivating and cultivating Litopenaeus vannamei fry.

Background technique

The prawn farming industry is the pillar industry of my country's aquaculture industry. Litopenaeus vannamei is the leading species of prawn cultured in my country. The annual farming area and output of Litopenaeus vannamei in my country have been stable at 250,000 hectares and 1.2 million tons for a long time. The annual demand for healthy and high-quality Litopenaeus seedlings exceeds 500 billion fish. Sustained and stable supply of healthy and high-quality seed is very important to ensure the sustainable development of my country's Litopenaeus vannamei aquaculture industry.

At present, the traditional seedling cultivation methods rely on manual control for water addition, temperature control, feeding, water change, etc., and depend on workers' sense of responsibility, hard work, and nursery technicians' experience in seedling feeding and water quality control. High, the demand for labor is large in the process of raising seedlings, the management process is relatively redundant, and the bait for seedling cultivation is mainly compound feed, the amount of residual bait is large, it is easy to corrupt and pollute the water quality, and the method of water quality control mainly depends on adding probiotics Bacteria, poor water quality control stability, therefore, the success rate of seedlings, the survival rate of seedlings and the stability of seedling quality are not high.

The seedling raising device designed by the present invention can realize automatic control of links such as water addition, temperature control, water change, and sewage discharge, which can save labor and facilitate fine management; While providing bait, it can also consume harmful substances such as ammonia nitrogen and nitrite nitrogen in the water body to purify the water quality. At the same time, the regular quantitative addition of probiotics, regular quantitative water changes and sewage discharge have formed a comprehensive water quality control method with 3 layers of treatment. Guaranteed to provide a stable growth and development environment for the cultivation of Litopenaeus vannamei seed, the success rate and stability of seed cultivation are high, and the cultivated seed is healthy and high-quality. The seedling cultivating device provided by the invention is simple in structure, reasonable in design, and can realize automatic operation in multiple links. Large-scale breeding of Litopenaeus seedlings, establishment of selective breeding families, and expansion of parental broodstock.

Contents of the invention

In order to overcome the problems of over-reliance on labor force, experience of technicians and instability of seedling water quality control in the existing Litopenaeus vannamei seed cultivation and breeding methods, the present invention provides a multi-link automatic operation, labor-saving, The operation is standardized, the water quality is controlled stably, and the device and method can be widely suitable for the large-scale breeding of Litopenaeus vannamei seedlings, the establishment of families, the expansion of parental broodstock, and the like.

In order to achieve the above object, the present invention adopts the following technical solutions:

A method for cultivating and cultivating Litopenaeus vannamei fry, characterized in that it comprises the following steps:

(1) Assembling and disinfection of seedling cultivation and breeding equipment: Assemble the breeding tank, connect the breeding tank and the reservoir, clean the breeding tank and the reservoir, disinfect with potassium permanganate, and use sterilized Wash the potassium permanganate with clean water;

(2) Seed cultivation and aquaculture water treatment: the water body is treated by sand filtration, fine filtration, organic matter removal, ultraviolet light, ozone and sodium hypochlorite disinfection, and heavy metal removal;

(3) Putting and feeding larvae: Add the treated culture water into the breeding tank, and put the larvae into the larvae at an appropriate density for cultivation and breeding;

(4) Water quality control for seed cultivation: regularly add probiotics to the breeding water, and regularly discharge sewage and change water.

The above step (1) described Litopenaeus vannamei seed cultivation and culture device includes a water storage tank, a culture tank, an aerator, an automatic temperature control heater, and the culture tank is equipped with water inlet, sewage discharge and water exchange devices , the angle between the breeding tank and the horizontal is 45~90 degrees, the bottom of the breeding tank is high and the middle is low, and the horizontal plane is 10~20 degrees inclined to the center of the bottom of the tank. The water changing and sewage discharge device includes a water pipe, a water changing pipe, and a sewage pipe. The lower end of the water pipe is provided with a sewage hole with an aperture range of 0.1 to 1.5 cm. The pipe body can wrap a plastic net with an aperture of 50 to 200 meshes. There are sewage holes with a pore size range of 0.1~1.5cm. Said assembling, at first connect the timing water valve with the water inlet pipe, and connect the reservoir and the culture tank with the water inlet pipe provided with the timing water valve, and connect the water inlet in the culture tank with a nozzle that can rotate freely 360 degrees; The water pipe is inserted into the water pipe installation circular groove at the bottom of the breeding tank, and then the water change pipe is placed in the water pipe, the upper end passes through the tank wall, and the lower end is connected to the sewage outlet. Sewage valve; finally, install the automatic temperature control heater and aerator in the cultivation tank, and connect the heater and aerator to the power supply respectively; when the cultivation device is assembled and cleaned, use potassium permanganate Disinfect, and rinse residual potassium permanganate with sterile clean water.

The steps and methods of seed cultivation and aquaculture water treatment described in the above step (2) are quartz sand filtration-foam separation method to remove organic matter and simultaneously carry out ozone disinfection-ultraviolet disinfection-oxidative chlorine disinfection for 6~24 hours-residual oxidative chlorine Removal - activated carbon adsorption of toxic and harmful substances - 0.5um precision filter filtration - oxygenation.

The larva delivery and feeding described in the above step (3) include the following steps:

① Adding water: according to the seedling cultivation and breeding water treatment steps and methods described in claim 3, seawater with a salinity range of 28-30% is processed and added to the breeding tank, and water is added for the first time to 50-100% of the water used for breeding seedlings in the tank ;

② Light source setting: set the light source on the breeding water surface, and keep the light intensity on the water surface at 1500~15000 lux;

③ Oxygenation: Turn on the oxygenation switch to keep the dissolved oxygen in the water above 5mg/L;

④ Water temperature control: Turn on the switch of the automatic temperature controller and set the automatic temperature control program: the temperature is set at 29~30°C for the larvae after 24 hours, and the temperature control accuracy is 0.5°C; the temperature is set at 31~31°C for the 25th hour~10 32°C, the temperature control accuracy is 0.5°C; after the 10th day, the temperature is set to 25~32°C, and the temperature control accuracy is 0.5°C;

⑤ Putting larvae: Put the larvae into the breeding tank in the package, and put the larvae in the package into the breeding water when the temperature difference between the water temperature in the package and the water in the breeding tank is lower than 0.5°C;

⑥ Feeding: The bait used for the cultivation of shrimp seedlings is single-celled diatoms and Artemia nauplii hatched after 16-20 hours. During the 5th stage of Litopenaeus vannamei nauplii to the 1st stage of psyllid larvae and the stage of mysis larvae, maintain the concentration of unicellular algae in the water body at 100-120/ml; The concentration of algae is 150~200/ml; Artemia larvae are fed from the late stage of the second stage of the zoa larvae, 6 times a day, once every 4 hours, and 1~2 for each stage 2 zoa. Artemia larvae/time, each zoan-like larvae of the third stage were fed 2~3/time, each mysis larvae of the first stage were fed 3~4 artemia larvae/time, each mysis larvae of the second stage were fed 4~5 Artemia larvae/time, each Mysis 3rd stage larva was fed 5~6 Artemia larvae/time, and each 1st stage postlarva was fed with more than 6 Artemia nauplii larvae each time.

The water quality control described in the above step (5) includes the following steps:

① According to the steps and methods for seed cultivation and aquaculture water treatment according to claim 3, seawater with a salinity range of 28-30% is processed and added to the reservoir, an automatic heating device is installed in the reservoir, and the automatic temperature controller is turned on Switch, press the right 4 step ⑤ to set the automatic temperature control program;

② Set the timing water valve switch program of the water inlet pipe. The amount of water added every day from the 2nd stage of the flea to the 1st stage of mysis larvae is 10-50% of the total seedling water body, and the daily water amount of the 2nd stage of mysis larvae to the larvae stage is the total seedling growth. 20~100% of the water body; the angle between the water inlet nozzle and the culture tank wall can be rotated when entering the water, and the water in the culture tank is driven to rotate through the water inlet, so that the dirt is concentrated to the center of the bottom of the tank;

③ Set the automatic blowdown valve switch, blowdown 1~15 minutes before each feeding;

④ Add probiotics such as Bacillus subtilis, lactic acid bacteria and EM bacteria to the water body from the zodiac stage 2 stage, add 0.5~2PPM/kind of probiotics every 2~4 days, and the content of probiotics exceeds ^1.0x108 /g ( ml).

Beneficial effects of the present invention:

1. The breeding device designed and adopted by the present invention has simple structure, reasonable layout, convenient and simple operation, and realizes automatic operation in multiple links such as water addition, temperature control, oxygenation, water exchange and sewage discharge, saving labor and cost, and is easier to realize Standardized operation.

2. The water quality control method designed and used in the present invention is ecologically stable, easier for seedling cultivation and aquaculture water environment stability, less disease occurrence, high seedling survival rate and success rate, and the cultivated seedlings are healthy and high-quality.

3. This seed breeding and breeding method is simple, efficient, and highly applicable, and is widely applicable to the large-scale cultivation of various Litopenaeus vannamei seedlings, the establishment of selected breeding families, medium cultivation, and the expansion of parents and parents. .

Detailed ways

The following specific examples are further detailed descriptions of the present invention.

Example 1: According to 1 cubic meter of seedling water body, 150,000 tails of Litopenaeus vannamei nauplius are put into each cubic water body to raise seedlings at a density

Seed cultivation and cultivation device and assembly:

Litopenaeus Benvana seedling cultivation and cultivating devices include a water storage tank, a culture tank, an aerator, an automatic temperature control heater, and the culture tank is provided with water inlet, sewage discharge and water changing devices, and the angle between the culture tank body and the horizontal is 45~90 degrees, the bottom of the breeding tank is high and the middle is low, and the horizontal plane is 10-20 degrees inclined to the center of the bottom of the tank. The water change and sewage discharge device includes a water pipe, a water change pipe, and a sewage pipe. The lower end of the water pipe is provided with a sewage hole with an aperture range of 0.1~1.5cm. The pipe body can be wrapped with a plastic net with an aperture of 50~200 mesh. Sewage holes with a range of 0.1~1.5cm.

First, connect the timing water valve with the water inlet pipe, and connect the reservoir and the breeding tank with the water inlet pipe provided with the timing water valve, and connect the water inlet in the breeding tank with a nozzle that can rotate freely in 360 degrees;

Secondly, insert the water pipe into the water pipe installation circular groove at the bottom of the breeding tank, and then put the water change pipe into the water pipe from the upper end through the tank wall, the lower end is connected to the sewage outlet, and the sewage pipe connected to the sewage outlet outside the tank Install timing blowdown valve on top;

Finally, install the automatic temperature control heater and aerator into the breeding tank, and connect the heater and aerator to the power supply respectively; when the breeding device is assembled and cleaned, it is disinfected with potassium permanganate and disinfected with sterilized Rinse residual potassium permanganate with clean water.

Seed cultivation and aquaculture water treatment:

The steps and methods are as follows: quartz sand filtration → foam separation method to remove organic matter and ozone disinfection → ultraviolet disinfection - 5PPM sodium hypochlorite disinfection for 12 hours → neutralize residual oxidative chlorine with sodium thiosulfate → activated carbon adsorption of toxic and harmful substances - 0.5um precision filtration filter → oxygenation.

Larva placement and feeding :

① Adding water: According to the above-mentioned seed cultivation and breeding water treatment method, seawater with a salinity range of 28~30% is treated and added to the breeding tank, and the water is added for the first time to 60% of the water used for breeding seedlings in the tank;

② Light source setting: set the light source on the breeding water surface, and keep the light intensity on the water surface at 3500 lux;

③ Oxygenation: Turn on the oxygenation switch to keep the dissolved oxygen in the water at 5mg/L;

④ Water temperature control: Turn on the automatic temperature controller switch and set the automatic temperature control program: the temperature is set at 29~30°C for the larvae for 24 hours, and the temperature control accuracy is 0.5°C; the temperature is set at 31~31°C for the 25th hour~10 32°C, the temperature control accuracy is 0.5°C; after the 10th day, the temperature is set to 25~32°C, and the temperature control accuracy is 0.5°C;

⑤ Putting larvae: Put the larvae into the breeding tank in the package, and put the larvae in the package into the breeding water when the temperature difference between the water temperature in the package and the water in the breeding tank is lower than 0.5°C;

⑥ Feeding: The bait used for the cultivation of shrimp seedlings is single-celled diatoms and artemia larvae hatched after 16-20 hours. During the 5th stage of Litopenaeus vannamei nauplii to the 1st stage of zoloid larvae and the stage of mysis larvae, maintain the concentration of unicellular algae in the water body at 100/ml; 180/ml; Feed Artemia larvae from the late stage 2 stage of zoa larvae, feed 6 times a day, feed once every 4 hours, and feed 1 Artemia larvae on average for each stage 2 stage zoa larvae , each stage 3 larvae of mysis was fed 2/time, each stage 1 mysis larva was fed 3 Artemia larvae/time, and each stage 2 larvae of mysis was fed 4 Artemia larvae/time, each Each mysis 3rd stage larva was fed with 5 Artemia larvae/time, and each 1st stage postlarva was fed with more than 6 Artemia nauplii larvae each time.

Water quality control :

① Treat seawater with a salinity range of 28-30% according to the above-mentioned steps and methods for seed cultivation and aquaculture water treatment and add it to the reservoir, install an automatic heating device in the reservoir, turn on the switch of the automatic temperature controller, and set The same automatic temperature control program for seedling cultivation;

② Set the timing water valve switch program of the water inlet pipe. The amount of water added every day from the 2nd stage of the flea to the 1st stage of mysis larvae is 20% of the total seedling water body, and the daily water amount of the 2nd stage of mysis larvae to the larval stage is 20% of the total seedling water body. 50%; the angle between the water inlet nozzle and the culture tank wall can be rotated when entering water, and the water in the culture tank is driven to rotate through the water inlet, so that the dirt is concentrated to the center of the tank bottom;

③ Set the automatic blowdown valve switch, blowdown for 3 minutes before each feeding.

④ Add probiotics such as Bacillus subtilis, lactic acid bacteria, and EM bacteria to the water body from the zodiac stage 2, and add 0.5PPM/type of probiotics every 4 days, and the content of probiotics exceeds ^1.0x108 /g (ml).

Example 2: According to 2 cubic meters of seedling water body, 350,000 tails of Litopenaeus vannamei nauplius are put into each cubic water body to raise seedlings at a density

Seed cultivation and cultivation device and assembly:

Litopenaeus Benvana seedling cultivation and cultivating devices include a water storage tank, a culture tank, an aerator, an automatic temperature control heater, and the culture tank is provided with water inlet, sewage discharge and water changing devices, and the angle between the culture tank body and the horizontal is 45~90 degrees, the bottom of the breeding tank is high and the middle is low, and the horizontal plane is 10-20 degrees inclined to the center of the bottom of the tank. The water change and sewage discharge device includes a water pipe, a water change pipe, and a sewage pipe. The lower end of the water pipe is provided with a sewage hole with an aperture range of 0.1~1.5cm. The pipe body can be wrapped with a plastic net with an aperture of 50~200 mesh. Sewage holes with a range of 0.1~1.5cm.

First, connect the timing water valve with the water inlet pipe, and connect the reservoir and the breeding tank with the water inlet pipe provided with the timing water valve, and connect the water inlet in the breeding tank with a nozzle that can rotate freely in 360 degrees;

Secondly, insert the water pipe into the water pipe installation circular groove at the bottom of the breeding tank, and then put the water change pipe into the water pipe from the upper end through the tank wall, the lower end is connected to the sewage outlet, and the sewage pipe connected to the sewage outlet outside the tank Install timing blowdown valve on top;

Finally, install the automatic temperature control heater and aerator into the breeding tank, and connect the heater and aerator to the power supply respectively; when the breeding device is assembled and cleaned, it is disinfected with potassium permanganate and disinfected with sterilized Rinse residual potassium permanganate with clean water.

Seed cultivation and aquaculture water treatment:

The steps and methods are as follows: quartz sand filtration→foam separation method to remove organic matter while ozone disinfection-ultraviolet disinfection-5PPM sodium hypochlorite disinfection for 12 hours→use sodium thiosulfate to neutralize residual oxidative chlorine→activated carbon adsorption of toxic and harmful substances-0.5um precision filtration filter → oxygenation.

Larva placement and feeding :

① Adding water: According to the above-mentioned seed cultivation and breeding water treatment method, the seawater with a salinity range of 28~30% is processed and added to the breeding tank, and the water is added for the first time to 80% of the water used for seedling cultivation in the tank;

② Light source setting: set the light source on the breeding water surface, and keep the light intensity on the water surface at 5000 lux;

③ Oxygenation: Turn on the oxygenation switch to keep the dissolved oxygen in the water at 6mg/L;

④ Water temperature control: Turn on the automatic temperature controller switch and set the automatic temperature control program: the temperature is set at 29~30°C for the larvae for 24 hours, and the temperature control accuracy is 0.5°C; the temperature is set at 31~31°C for the 25th hour~10 32°C, the temperature control accuracy is 0.5°C; after the 10th day, the temperature is set to 25~32°C, and the temperature control accuracy is 0.5°C;

⑤ Putting larvae: Put the larvae into the breeding tank in the package, and put the larvae in the package into the breeding water when the temperature difference between the water temperature in the package and the water in the breeding tank is lower than 0.5°C;

⑥ Feeding: The bait used for the cultivation of shrimp seedlings is single-celled diatoms and artemia larvae hatched after 16-20 hours. During the 5th stage of Litopenaeus vannamei nauplii to the 1st stage of zoloid larvae and the stage of mysis larvae, the concentration of unicellular algae in the water body is maintained at 120/ml; 200/ml; Feed Artemia larvae from the late stage 2 stage of zoa larvae, feed 6 times a day, feed once every 4 hours, and feed each stage 2 zoa larvae on average 2 Artemia larvae/time , each stage 3 larvae of mysis was fed 3/time, each stage 1 mysis larva was fed 4 Artemia larvae/time, and each stage 2 larvae of mysis was fed 6 Artemia larvae/time, each Each mysis 3rd-stage larva was fed with 6 Artemia larvae/time, and each 1st-stage postlarva was fed with more than 6 Artemia nauplii larvae each time.

Water quality control :

① Treat seawater with a salinity range of 28-30% according to the above-mentioned steps and methods for seed cultivation and aquaculture water treatment and add it to the reservoir, install an automatic heating device in the reservoir, turn on the switch of the automatic temperature controller, and set The same automatic temperature control program for seedling cultivation;

② Set the timing water valve switch program of the water inlet pipe. The amount of water added every day from the 2nd stage of the flea to the 1st stage of mysis larvae is 40% of the total seedling water body, and the daily water amount of the 2nd stage of mysis larvae to the larval stage is 40% of the total seedling water body. 80%; the angle between the water inlet nozzle and the culture tank wall can be rotated when entering water, and the water in the culture tank is driven to rotate through the water inlet, so that the dirt is concentrated to the center of the bottom of the tank;

③ Set the automatic blowdown valve switch, blowdown for 5 minutes before each feeding.

④ Add probiotics such as Bacillus subtilis, lactic acid bacteria, and EM bacteria to the water body from the zodiac stage 2, and add 1PPM/type of probiotics every 2 days, and the content of probiotics exceeds ^1.0x108 /g (ml).

Example 3: According to 3 cubic meters of seedling water body, 500,000 tails of Litopenaeus vannamei nauplius are put into each cubic water body to raise seedlings at a density

Seed cultivation and cultivation device and assembly:

Litopenaeus Benvana seedling cultivation and cultivating devices include a water storage tank, a culture tank, an aerator, an automatic temperature control heater, and the culture tank is provided with water inlet, sewage discharge and water changing devices, and the angle between the culture tank body and the horizontal is 45~90 degrees, the bottom of the breeding tank is high and the middle is low, and the horizontal plane is 10~20 degrees inclined to the center of the bottom of the tank. The water change and sewage discharge device includes a water pipe, a water change pipe, and a sewage pipe. The lower end of the water pipe is provided with a sewage hole with an aperture range of 0.1~1.5cm. The pipe body can be wrapped with a plastic net with an aperture of 50~200 mesh. Sewage holes with a range of 0.1~1.5cm.

First, connect the timing water valve with the water inlet pipe, and connect the reservoir and the breeding tank with the water inlet pipe provided with the timing water valve, and connect the water inlet in the breeding tank with a nozzle that can rotate freely in 360 degrees;

Secondly, insert the water pipe into the water pipe installation circular groove at the bottom of the breeding tank, and then put the water change pipe into the water pipe from the upper end through the tank wall, the lower end is connected to the sewage outlet, and the sewage pipe connected to the sewage outlet outside the tank Install timing blowdown valve on top;

Finally, install the automatic temperature control heater and aerator into the breeding tank, and connect the heater and aerator to the power supply respectively; when the breeding device is assembled and cleaned, it is disinfected with potassium permanganate and disinfected with sterilized Rinse residual potassium permanganate with clean water.

Seed cultivation and aquaculture water treatment:

The steps and methods are as follows: quartz sand filtration→foam separation method to remove organic matter while ozone disinfection-ultraviolet disinfection-5PPM sodium hypochlorite disinfection for 12 hours→use sodium thiosulfate to neutralize residual oxidative chlorine→activated carbon adsorption of toxic and harmful substances-0.5um precision filtration filter → oxygenation.

Larva placement and feeding :

① Adding water: According to the above-mentioned seed cultivation and breeding water treatment method, the seawater with a salinity range of 28~30% is treated and added to the breeding tank, and the water is added for the first time to 100% of the tank water for seedlings;

② Light source setting: set the light source on the breeding water surface, and keep the light intensity on the water surface at 7000 lux;

③ Oxygenation: Turn on the oxygenation switch to keep the dissolved oxygen in the water at 7mg/L;

④ Water temperature control: Turn on the automatic temperature controller switch and set the automatic temperature control program: the temperature of the larvae is set at 29~30°C for 24 hours, and the temperature control accuracy is 0.5°C; the temperature is set at 31~32°C for the 25th hour~10 days °C, the temperature control accuracy is 0.5 °C; after the 10th day, the temperature is set to 25~32 °C, and the temperature control accuracy is 0.5 °C;

⑤ Putting larvae: Put the larvae into the breeding tank in the package, and put the larvae in the package into the breeding water when the temperature difference between the water temperature in the package and the water in the breeding tank is lower than 0.5°C;

⑥ Feeding: The bait used for the cultivation of shrimp seedlings is single-celled diatoms and artemia larvae hatched after 16-20 hours. During the 5th stage of Litopenaeus vannamei nauplii to the 1st stage of zoloid larvae and the stage of mysis larvae, the concentration of unicellular algae in the water body is maintained at 120/ml; 200/ml; Feed Artemia larvae from the late stage 2 of Zleuform larvae, feed 6 times a day, feed once every 4 hours, and feed each Artemia 2nd stage larvae on average 1 Artemia larvae/time , each stage 3 larvae of mysis was fed 2/time, each stage 1 mysis larva was fed 3 Artemia larvae/time, and each stage 2 larvae of mysis was fed 4 Artemia larvae/time, each Each mysis 3rd stage larva was fed with 5 Artemia larvae/time, and each 1st stage postlarva was fed with more than 6 Artemia nauplii larvae each time.

Water quality control :

① Treat seawater with a salinity range of 28-30% according to the above-mentioned steps and methods for seed cultivation and aquaculture water treatment and add it to the reservoir, install an automatic heating device in the reservoir, turn on the switch of the automatic temperature controller, and set The same automatic temperature control program for seedling cultivation;

② Set the timing water valve switch program of the water inlet pipe. The amount of water added every day from the 2nd stage of the flea to the 1st stage of mysis larvae is 20% of the total seedling water body, and the daily water amount of the 2nd stage of mysis larvae to the larval stage is 20% of the total seedling water body. 50%; the angle between the water inlet nozzle and the culture tank wall can be rotated when entering water, and the water in the culture tank is driven to rotate through the water inlet, so that the dirt is concentrated to the center of the tank bottom;

③ Set the automatic blowdown valve switch, blowdown for 8 minutes before each feeding.

④ Add probiotics such as Bacillus subtilis, lactic acid bacteria, and EM bacteria to the water body from the zodiac stage 2, and add 1.5PPM/type of probiotics every 2 days, and the content of probiotics exceeds ^1.0x108 /g (ml).

The above-mentioned embodiments are only one of the more preferred specific modes of the present invention, and ordinary changes and replacements performed by those skilled in the art within the scope of the technical solution of the present invention shall be included in the protection scope of the present invention.

Claims (5)

1. A method for cultivating and cultivating Litopenaeus vannamei seed, characterized in that: comprising seed cultivating and cultivating device assembly and disinfection, seed cultivation and cultivating water treatment, larvae throwing in and feeding, and seed cultivation water quality control ,Proceed as follows: (1) Assembling and disinfection of seedling cultivation and breeding equipment: Assemble the breeding tank, connect the breeding tank and the reservoir, clean the breeding tank and the reservoir, disinfect with potassium permanganate, and use sterilized Rinse with clean water to remove residual potassium permanganate; (2) Seed cultivation and aquaculture water treatment: the water body is treated by sand filtration, fine filtration, organic matter removal, ultraviolet light, ozone and sodium hypochlorite disinfection, and heavy metal removal; (3) Putting and feeding larvae: Add the treated culture water into the breeding tank, and put the larvae into the larvae at an appropriate density for cultivation and breeding; (4) Water quality control for seed cultivation: regularly add probiotics to the breeding water, and regularly discharge sewage and change water. 2. The method for cultivating and cultivating Litopenaeus vannamei seed according to claim 1, characterized in that: the cultivation and cultivating device for Litopenaeus vannamei seed described in step (1) includes a water storage tank, a culture tank, an increasing Oxygenator, automatic temperature control heater, described breeding tank is provided with water inlet, blowdown and water changing device, and the included angle of breeding tank body and horizontal is 45～90 degree, and the four sides of breeding tank bottom are high in the middle and low, and the horizontal plane is 10-20 degrees inclined to the center of the bottom of the tank, the center of the bottom of the breeding tank is provided with a sewage hole and a circular groove for installing a water pipe; the water change and sewage discharge device includes a water pipe, a water change pipe, and a sewage pipe, and the lower end of the water pipe is provided with an aperture range 0.1-1.5cm sewage hole, the pipe body can be wrapped with a plastic mesh with an aperture of 50-200 mesh, and the lower end of the water change pipe is provided with a sewage discharge hole with an aperture range of 0.1-1.5cm; the above-mentioned assembly, first connect the timing water valve with the water inlet pipe, and Connect the water storage tank and the breeding tank with a water inlet pipe provided with a timing water valve, and the water inlet in the breeding tank is connected to a nozzle that can rotate freely at 360 degrees; secondly, insert the water pipe into the water pipe installation circular groove at the bottom of the breeding tank, and then insert The water change pipe is placed in the water pipe, the upper end of which passes through the cylinder wall, and the lower end is connected to the sewage outlet, and a timing sewage valve is installed on the sewage pipe connected to the sewage outlet outside the cylinder body; finally, the automatic temperature control heater and the booster The oxygenator is installed in the culture tank, and the heater and the oxygenator are connected to the power supply respectively; after the culture device is assembled and cleaned, it is disinfected with potassium permanganate, and the residual potassium permanganate is rinsed with sterilized clean water. 3. The method for cultivating and cultivating Litopenaeus vannamei seedlings according to claim 1, characterized in that: the step and method of seedling cultivating and cultivating water treatment in step (2) are quartz sand filtration→foam separation method Remove organic matter and carry out ozone disinfection at the same time→ultraviolet disinfection→oxidative chlorine disinfection→residual oxidative chlorine removal→activated carbon adsorption of toxic and harmful substances-0.5um precision filter filtration→oxygenation. 4. The method for cultivating and cultivating Litopenaeus vannamei seedlings according to claim 1, characterized in that: the step (3) of placing and feeding the larvae comprises the following steps: ① Adding water: according to the seedling cultivation and breeding water treatment steps and methods described in claim 3, seawater with a salinity range of 28-30% is processed and added to the breeding tank, and water is added for the first time to 50-100% of the water used for breeding seedlings in the tank ; ② Light source setting: set the light source on the breeding water surface, and keep the light intensity on the water surface at 1500~15000 lux; ③ Oxygenation: Turn on the oxygenation switch to keep the dissolved oxygen in the water above 5mg/L; ④ Water temperature control: Turn on the switch of the automatic temperature controller and set the automatic temperature control program: the temperature is set at 29~30°C for the larvae after 24 hours, and the temperature control accuracy is 0.5°C; the temperature is set at 31~31°C for the 25th hour~10 32°C, the temperature control accuracy is 0.5°C; after the 10th day, the temperature is set to 25~32°C, and the temperature control accuracy is 0.5°C; ⑤ Putting larvae: Put the larvae into the breeding tank in the package, and put the larvae in the package into the breeding water when the temperature difference between the water temperature in the package and the water in the breeding tank is lower than 0.5°C; ⑥ Feeding: The bait used for the cultivation of shrimp seedlings is unicellular diatoms and Artemia nauplii hatched after 16-20 hours; In the larvae stage, maintain the concentration of 100-150 unicellular algae/ml in the water body; maintain the concentration of 120-200 unicellular algae/ml in the water body for the 2nd to 3rd stage of zoan larvae; For larvae, feed 6 times a day, once every 4 hours, feed 1-2 artemia larvae for each stage 2 flea-like larvae, and feed 2-3 larvae for stage 3 flea-like larvae/time Each mysis larva at the first stage was fed with 3~4 Artemia larvae/time, each mysis larva at the second stage was fed with 4~5 Artemia larvae/time, and each mysis larva at the third stage was fed with 5 ~6 artemia larvae/time, and each 1st-stage postlarva was fed more than 6 artemia nauplii larvae each time. 5. The method for cultivating and cultivating Litopenaeus vannamei seedlings according to claim 1, characterized in that: the water quality control in step (4) includes the following steps: ① According to the steps and methods for seed cultivation and aquaculture water treatment according to claim 3, seawater with a salinity range of 28-30% is processed and added to the reservoir, an automatic heating device is installed in the reservoir, and the automatic temperature controller is turned on Switch, press the right 4 step ⑤ to set the automatic temperature control program; ② Set the timing water valve switch program of the water inlet pipe. The amount of water added every day from the 2nd stage of the flea to the 1st stage of mysis larvae is 10-50% of the total seedling water body, and the daily water amount of the 2nd stage of mysis larvae to the larvae stage is the total seedling growth. 20~100% of the water body; the angle between the water inlet nozzle and the culture tank wall can be rotated when entering the water, and the water in the culture tank is driven to rotate through the water inlet, so that the dirt is concentrated to the center of the bottom of the tank; ③ Set the automatic blowdown valve switch, blowdown 1~15 minutes before each feeding; ④ Add probiotics such as Bacillus subtilis, lactic acid bacteria and EM bacteria to the water body from the zodiac stage 2 stage, add 0.5~2PPM/kind of probiotics every 2~4 days, and the content of probiotics exceeds ^1.0x108 /g ( ml).