CN104126567A - Method for controlling stomaphis sinisalicis hid in trunks through spreading pandora neoaphidis by using ants - Google Patents
Method for controlling stomaphis sinisalicis hid in trunks through spreading pandora neoaphidis by using ants Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 24
- 241000257303 Hymenoptera Species 0.000 title abstract description 31
- 241001289560 Pandora neoaphidis Species 0.000 title abstract description 3
- 241000420562 Stomaphis sinisalicis Species 0.000 title description 2
- 241001124076 Aphididae Species 0.000 claims abstract description 97
- 241000124033 Salix Species 0.000 claims abstract description 47
- 239000000843 powder Substances 0.000 claims abstract description 15
- 210000003323 beak Anatomy 0.000 claims abstract description 12
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 claims description 7
- 240000002495 Cucumis melo var. inodorus Species 0.000 claims description 7
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 5
- 235000013305 food Nutrition 0.000 claims 2
- 238000001816 cooling Methods 0.000 claims 1
- 238000005057 refrigeration Methods 0.000 claims 1
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- 241000256593 Brachycaudus schwartzi Species 0.000 abstract description 3
- 241000165113 Myzus ligustri Species 0.000 abstract 1
- 230000001112 coagulating effect Effects 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 abstract 1
- 210000002615 epidermis Anatomy 0.000 abstract 1
- 239000005906 Imidacloprid Substances 0.000 description 9
- YWTYJOPNNQFBPC-UHFFFAOYSA-N imidacloprid Chemical compound [O-][N+](=O)\N=C1/NCCN1CC1=CC=C(Cl)N=C1 YWTYJOPNNQFBPC-UHFFFAOYSA-N 0.000 description 9
- 229940056881 imidacloprid Drugs 0.000 description 9
- 238000005507 spraying Methods 0.000 description 8
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000007921 spray Substances 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- 241000224016 Plasmodium Species 0.000 description 3
- 241000899950 Salix glauca Species 0.000 description 2
- 239000004563 wettable powder Substances 0.000 description 2
- 208000032544 Cicatrix Diseases 0.000 description 1
- 241000255749 Coccinellidae Species 0.000 description 1
- 241000258923 Neuroptera Species 0.000 description 1
- 241000218998 Salicaceae Species 0.000 description 1
- 235000002493 Salix babylonica Nutrition 0.000 description 1
- 241000734985 Salix x sepulcralis Species 0.000 description 1
- 241001481659 Syrphidae Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
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- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
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Abstract
本发明公开了一种利用蚂蚁传播蚜虫疠霉Pandoraneoaphidis防治树干隐匿柳长喙大蚜的方法，首先准备红糖猪油块，使红糖含量为5-20%，凝结成块状后，切成0.5-2cm3见方的小块，然后使其在蚜虫疠霉孢子粉中滚一下，做成孢子释放块。将其放置在蚁巢出口和树干蚁道入口处，供蚂蚁自由取食，蚂蚁取食过程中，会将蚜虫疠霉孢子沾在表皮、口器和四肢上，当其与蚁道中隐匿的柳长喙大蚜接触时，将孢子传给柳长喙大蚜，柳长喙大蚜体表感染蚜虫疠霉孢子后，感病死亡。本发明防治效果好，成本低，环保。
The invention discloses a method for using ants to spread the aphid mold Pandoraneoaphidis to prevent and control the hidden willow beak aphid in the trunk. Firstly, brown sugar lard blocks are prepared to make the brown sugar content 5-20%. After coagulating into blocks, they are cut into 0.5- 2cm 3 square small piece, then make it roll in aphid spore powder, make spore release block. Place it at the exit of the ant nest and the entrance of the trunk ant tunnel for the ants to eat freely. During the feeding process of the ants, the aphid spores will stick to the epidermis, mouthparts and limbs. When aphids come into contact with them, the spores will be transmitted to A. aphids. After being infected with spores of P. aphids, the aphids will be infected and die. The invention has good control effect, low cost and environmental protection.
Description
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技术领域 technical field
本发明属于生物技术领域，涉及一种利用蚂蚁传播蚜虫疠霉Pandora neoaphidis防治树干隐匿柳长喙大蚜Stomaphis sinisalicis的方法。 The invention belongs to the field of biotechnology, and relates to a method for preventing and controlling Stomaphis sinisalicis in tree trunks by using ants to spread aphid mold Pandora neoaphidis .
背景技术 Background technique
柳长喙大蚜是一种重要的柳树害虫，主要危害白柳、毛柳、垂柳、爆竹柳、青冈柳、光滑柳、朝鲜柳等多种柳树。该蚜一年发生10多代，以成虫在主干下部的树皮缝隙内越冬。翌年春季开始向上部活动，4-5月大量繁殖盛发，形成灾害。大量发生时，所分泌的蜜露如下微雨，地面上有层褐色黏液。若蚜和成虫多群集在幼枝分杈处和嫩枝上危害，吸食枝液，分泌蜜露，常引起煤污病发生，盛夏较少，形成春季和秋季两个发生盛期。部分虫体可寄生于柳树的树缝中、疤痕处或根部土壤中，以口针刺吸到树干的韧皮部吸收柳树的营养，在树干上，一般有共生蚂蚁以泥土修筑的蚁道，在根部，柳长喙大蚜则直接隐藏于土壤中进行生活，免受蚜虫天敌如瓢虫、草蛉、食蚜蝇等昆虫的捕食，同时更难感染昆虫病原体，使用农药进行防治也很难奏效，防治难度极大。同时，隐匿型的柳长喙大蚜是其越冬的重要途径，也是柳长喙大蚜难以防治的重要原因之一。 Willow long beak aphid is an important willow pest, mainly harming many kinds of willows such as white willow, hairy willow, weeping willow, firecracker willow, Qinggang willow, smooth willow and Korean willow. The aphid has more than 10 generations a year and overwinters as adults in the bark crevices at the lower part of the trunk. In the spring of the following year, it begins to move upwards, and it breeds in large numbers from April to May, causing disasters. When a large number occurs, the secreted honeydew is like light rain, and there is a layer of brown mucus on the ground. Nymphs and adults cluster on young branches and twigs, suck branch sap, and secrete honeydew, often causing sooty spot, less in midsummer, forming two peak occurrences in spring and autumn. Some worms can parasitize in the crevices, scars or root soil of willow trees. They use needles to suck into the phloem of the trunk to absorb the nutrients of willow trees. On the trunk, there are generally ant tunnels built by symbiotic ants with soil. The willow beaked aphid lives directly hidden in the soil, avoiding the predation of natural enemies of aphids such as ladybugs, lacewings, hoverflies and other insects, and at the same time it is more difficult to infect insect pathogens, and it is difficult to use pesticides to control it. Prevention is extremely difficult. At the same time, the cryptic aphid is an important way for it to overwinter, and it is also one of the important reasons why it is difficult to control the aphid.
发明内容 Contents of the invention
本发明针对以上不足之处，提供了一种利用蚂蚁传播蚜虫疠霉防治树干隐匿柳长喙大蚜的方法，通过与柳长喙大蚜共生的蚂蚁将蚜虫疠霉传播给柳长喙大蚜，柳长喙大蚜体表感染蚜虫疠霉孢子后，感病死亡，来达到对柳长喙大蚜的防治。 The present invention aims at the above deficiencies, and provides a method of using ants to spread Plagus aphids to prevent and control the hidden willow beaked aphid in tree trunks, and spread the aphids aphids to aphids aphids through symbiotic ants , after the body surface of the willow beaked aphid is infected with the spores of aphid spores, it is infected and dies, so as to achieve the control of the willow long beaked aphid.
本发明解决其技术问题所采用的技术方案是：一种利用蚂蚁传播蚜虫疠霉防治树干隐匿柳长喙大蚜的方法，包含有如下步骤： The technical solution adopted by the present invention to solve the technical problem is: a method for using ants to spread aphids Placiformis to prevent and control tree trunk hidden willow long-billed aphids, comprising the following steps:
（1）准备蚜虫疠霉孢子粉，可通过购买或固体发酵培养获得； (1) Prepare aphid spore powder, which can be obtained by purchasing or solid fermentation culture;
（2）准备红糖猪油块 先将猪油融化，慢慢加入红糖融化，轻轻搅匀，使红糖含量占5-20%，冷却、凝结后做成红糖猪油块，将红糖猪油块切成0.5-2cm3见方的小块，冰箱内4℃冷藏备用； (2) To prepare the brown sugar lard block, first melt the lard, slowly add brown sugar to melt, stir gently, so that the brown sugar content accounts for 5-20%, cool and condense to make brown sugar lard block, brown sugar lard block Cut into small pieces of 0.5-2cm 3 squares, and refrigerate at 4°C for later use;
（3）将切成小块的红糖猪油块在蚜虫疠霉孢子粉中滚动，使表面沾满孢子粉，做成蚜虫疠霉孢子释放块； (3) Roll the brown sugar lard block cut into small pieces in the spore powder of aphid spores, so that the surface is covered with spore powder to make a spore release block of spores of aphid spores;
（4）将蚜虫疠霉孢子释放块放置在树干有蚁道的柳树周围、与柳长喙大蚜共生的蚂蚁的蚁道口和蚁窝口，蚂蚁取食蚜虫疠霉孢子释放块的过程中，将蚜虫疠霉孢子沾在体表或口器或四肢上，蚂蚁在与蚁道内隐藏的柳长喙大蚜接触或取食蜜露时，将蚜虫疠霉孢子传给柳长喙大蚜，柳长喙大蚜接触蚜虫疠霉孢子后，感病死亡。 (4) Place the spore release block of Aphidia spores around the willow tree with ant tunnels in the trunk, at the entrance of the ant tunnel and ant nest of the ants that live in symbiosis with A. Stain the spores of the aphid spores on the body surface or on the mouthparts or limbs, and when the ants come into contact with the hidden aphids in the ant tunnel or eat honeydew, they will pass the spores to the spores of the aphids, the willow After being exposed to the spores of P. aphididae, the long-billed aphid is infected and dies.
进一步的，第一次在树干有蚁道的柳树周围、与柳长喙大蚜共生的蚂蚁的蚁道口和蚁窝口，放置蚜虫疠霉孢子释放块10天后，再放置一次蚜虫疠霉孢子释放块，防治效果更好。 Further, for the first time around willow trees with ant tunnels in the trunk, at the ant tunnel openings and ant nest openings of ants that live in symbiotic relationship with A. blocks, the control effect is better.
本发明主要是利用了蚂蚁和柳长喙大蚜之间的共生关系，利用在外活动的蚂蚁将致病蚜虫疠霉孢子传播给在树缝中隐匿的柳长喙大蚜，只需制作蚜虫疠霉孢子释放块即可实现本发明，防治成本低，使用蚜虫疠霉孢子引起柳长喙大蚜发病进行柳长喙大蚜的防治，不使用其他化学药剂，不破坏生态环境，环保； The present invention mainly utilizes the symbiotic relationship between ants and Aphid aphid, and uses the ants that are active outside to spread the pathogenic aphid spores to the Aphid aphid hiding in the crevices of trees. The mold spore release block can realize the present invention, and the control cost is low. Using the mold spores of aphids to cause the occurrence of Aphid aphidus to prevent and treat A. aphid, does not use other chemicals, does not destroy the ecological environment, and is environmentally friendly;
实验证明，在树干有蚁道的柳树周围、与柳长喙大蚜共生的蚂蚁的蚁道口和蚁窝口，两次间隔放置蚜虫疠霉孢子释放块20天后，柳长喙大蚜感染率在78%，防治效果与直接剖开树道喷洒蚜虫疠霉孢子的效果一样好。本方法省时、省力，成本低廉，且事先无需调查蚁道内的蚜虫数量，更无需剖开蚁道对其中的柳长喙大蚜进行杀灭，只需在柳长喙大蚜共生蚁的蚁巢口或树干蚁道口放置蚜虫疠霉孢子释放块，就可达到防治柳长喙大蚜的目的，该方法不需要专业培训既可完成操作，并能达到同样的防治效果，有很强的可推广性，是一种有推广前景的柳长喙大蚜生物防治新方法。 Experiments have shown that around willow trees with ant tunnels in their trunks, at the entrances of ant tunnels and ant nests of ants that live in symbiotic relationship with A. 78%, the control effect is as good as the effect of directly cutting the tree path and spraying aphid spores. This method is time-saving, labor-saving and low-cost, and there is no need to investigate the number of aphids in the ant tunnel in advance, and it is not necessary to cut open the ant tunnel to kill the willow beaked aphid. Placing the release block of aphid spores at the mouth of the nest or the ant crossing of the trunk can achieve the purpose of preventing and controlling the willow beak aphid. This method can complete the operation without professional training and can achieve the same control effect. It has strong reliability. It is a new method of biological control of the willow beak aphid with promotional prospects.
附图说明 Description of drawings
图1所示为蚜虫疠霉不同防治方法对树干蚁道内隐匿型柳长喙大蚜的控制效果； Figure 1 shows the control effect of different control methods of aphid Placiformis on the hidden type willow long beaked aphid in the ant tunnel of the trunk;
图2 所示为吡虫啉对树干蚁道内隐匿型柳长喙大蚜的控制效果。 Figure 2 shows the control effect of imidacloprid on the occult Aphid aphid in tree trunk ant tunnels.
具体实施方式 Detailed ways
实施例1 Example 1
一种利用蚂蚁传播蚜虫疠霉防治树干隐匿柳长喙大蚜的方法，包含有如下步骤： A method for using ants to spread aphids and prevent and control tree trunk hidden willow beaked aphids, comprising the following steps:
（1）准备蚜虫疠霉孢子粉，可通过购买或固体发酵培养获得； (1) Prepare aphid spore powder, which can be obtained by purchasing or solid fermentation culture;
（2）准备红糖猪油块 先将猪油融化，慢慢加入红糖融化，轻轻搅匀，使红糖含量占5%，冷却、凝结后做成红糖猪油块，将红糖猪油块切成0.5cm3见方的小块，冰箱内4℃冷藏备用； (2) Prepare the brown sugar lard block first melt the lard, slowly add the brown sugar to melt, stir gently, so that the brown sugar content accounts for 5%, cool and condense to make brown sugar lard block, cut the brown sugar lard block into Small pieces of 0.5cm 3 square, refrigerated at 4°C for later use;
（3）将切成小块的红糖猪油块在蚜虫疠霉孢子粉中滚动，使表面沾满孢子粉，做成蚜虫疠霉孢子释放块； (3) Roll the brown sugar lard block cut into small pieces in the spore powder of aphid spores, so that the surface is covered with spore powder to make a spore release block of spores of aphid spores;
（4）将蚜虫疠霉孢子释放块放置在树干有蚁道的柳树周围、与柳长喙大蚜共生的蚂蚁的蚁道口和蚁窝口，还可在第一次放置蚜虫疠霉孢子释放块10天后，再放置一次蚜虫疠霉孢子释放块，蚂蚁会被红糖吸引进行取食，蚂蚁取食蚜虫疠霉孢子释放块的过程中，将蚜虫疠霉孢子沾在体表或口器或四肢上，蚂蚁在与蚁道内隐藏的柳长喙大蚜接触或取食蜜露时，将蚜虫疠霉孢子传给柳长喙大蚜，柳长喙大蚜接触蚜虫疠霉孢子后，感病死亡， (4) Place the release block of aphid spores around willow trees with ant tunnels in the trunk, at the ant tunnel and ant nest openings of ants that live in symbiosis with the willow aphid. After 10 days, place the Aphidia spore release block again, and the ants will be attracted by the brown sugar to eat. During the process of eating the Aphidia spore release block, the ants will stick the Aphidia spores on the body surface or mouthparts or limbs , when the ants come into contact with the Aphidia spp. hidden in the ant tunnel or feed on honeydew, they will pass the spores of P.
实施例2 Example 2
一种利用蚂蚁传播蚜虫疠霉防治树干隐匿柳长喙大蚜的方法，包含有如下步骤： A method for using ants to spread aphids and prevent and control tree trunk hidden willow beaked aphids, comprising the following steps:
（1）准备蚜虫疠霉孢子粉，可通过购买或固体发酵培养获得； (1) Prepare aphid spore powder, which can be obtained by purchasing or solid fermentation culture;
（2）准备红糖猪油块 先将猪油融化，慢慢加入红糖融化，轻轻搅匀，使红糖含量占20%，冷却、凝结后做成红糖猪油块，将红糖猪油块切成2cm3见方的小块，冰箱内4℃冷藏备用； (2) Prepare the brown sugar lard block first melt the lard, slowly add brown sugar to melt, stir gently to make the brown sugar content account for 20%, cool and condense to make brown sugar lard block, cut the brown sugar lard block into Small pieces of 2cm 3 square, refrigerated at 4°C for later use;
（3）将切成小块的红糖猪油块在蚜虫疠霉孢子粉中滚动，使表面沾满孢子粉，做成蚜虫疠霉孢子释放块； (3) Roll the brown sugar lard block cut into small pieces in the spore powder of aphid spores, so that the surface is covered with spore powder to make a spore release block of spores of aphid spores;
（4）将蚜虫疠霉孢子释放块放置在树干有蚁道的柳树周围、与柳长喙大蚜共生的蚂蚁的蚁道口和蚁窝口，还可在第一次放置蚜虫疠霉孢子释放块10天后，再放置一次蚜虫疠霉孢子释放块，蚂蚁会被红糖吸引进行取食，蚂蚁取食蚜虫疠霉孢子释放块的过程中，将蚜虫疠霉孢子沾在体表或口器或四肢上，蚂蚁在与蚁道内隐藏的柳长喙大蚜接触或取食蜜露时，将蚜虫疠霉孢子传给柳长喙大蚜，柳长喙大蚜接触蚜虫疠霉孢子后，感病死亡， (4) Place the release block of aphid spores around willow trees with ant tunnels in the trunk, at the ant tunnel and ant nest openings of ants that live in symbiosis with the willow aphid. After 10 days, place the Aphidia spore release block again, and the ants will be attracted by the brown sugar to eat. During the process of eating the Aphidia spore release block, the ants will stick the Aphidia spores on the body surface or mouthparts or limbs , when the ants come into contact with the Aphidia spp. hidden in the ant tunnel or feed on honeydew, they will pass the spores of P.
实施例3 Example 3
一种利用蚂蚁传播蚜虫疠霉防治树干隐匿柳长喙大蚜的方法，包含有如下步骤： A method for using ants to spread aphids and prevent and control tree trunk hidden willow beaked aphids, comprising the following steps:
（1）准备蚜虫疠霉孢子粉，可通过购买或固体发酵培养获得； (1) Prepare aphid spore powder, which can be obtained by purchasing or solid fermentation culture;
（2）准备红糖猪油块 先将猪油融化，慢慢加入红糖融化，轻轻搅匀，使红糖含量占12%，冷却、凝结后做成红糖猪油块，将红糖猪油块切成1cm3见方的小块，冰箱内4℃冷藏备用； (2) Prepare the brown sugar lard block first melt the lard, slowly add brown sugar to melt, stir gently to make the brown sugar content account for 12%, cool and condense to make brown sugar lard block, cut the brown sugar lard block into Small pieces of 1cm 3 square, refrigerated at 4°C for later use;
（3）将切成小块的红糖猪油块在蚜虫疠霉孢子粉中滚动，使表面沾满孢子粉，做成蚜虫疠霉孢子释放块； (3) Roll the brown sugar lard block cut into small pieces in the spore powder of aphid spores, so that the surface is covered with spore powder to make a spore release block of spores of aphid spores;
（4）将蚜虫疠霉孢子释放块放置在树干有蚁道的柳树周围、与柳长喙大蚜共生的蚂蚁的蚁道口和蚁窝口，还可在第一次放置蚜虫疠霉孢子释放块10天后，再放置一次蚜虫疠霉孢子释放块，蚂蚁会被红糖吸引进行取食，蚂蚁取食蚜虫疠霉孢子释放块的过程中，将蚜虫疠霉孢子沾在体表或口器或四肢上，蚂蚁在与蚁道内隐藏的柳长喙大蚜接触或取食蜜露时，将蚜虫疠霉孢子传给柳长喙大蚜，柳长喙大蚜接触蚜虫疠霉孢子后，感病死亡， (4) Place the release block of aphid spores around willow trees with ant tunnels in the trunk, at the ant tunnel and ant nest openings of ants that live in symbiosis with the willow aphid. After 10 days, place the Aphidia spore release block again, and the ants will be attracted by the brown sugar to eat. During the process of eating the Aphidia spore release block, the ants will stick the Aphidia spores on the body surface or mouthparts or limbs , when the ants come into contact with the Aphidia spp. hidden in the ant tunnel or feed on honeydew, they will pass the spores of P.
应用实例： Applications:
以本发明的方法，在白柳上进行应用，取有树干有泥土蚁道的柳树，分别做以下处理：（1）利用本发明方法通过蚂蚁携带蚜虫疠霉孢子释放块防治柳长喙大蚜；（2）利用本发明的方法，分别在第一次放置蚜虫疠霉孢子释放块后10 d 再次放置一次；（3）在蚁道表面喷洒浓度为108个/ml的蚜虫疠霉孢子；（4）剖开蚁道喷洒浓度为108个/ml的蚜虫疠霉孢子；（5）在蚁道表面喷洒稀释1000倍5%的吡虫啉可湿性粉剂；（6）剖开蚁道喷洒稀释1000倍5%的吡虫啉可湿性粉剂；（7）对照，不做任何处理。分别于处理后10 d和20d剖开蚁道进行调查蚁道内柳长喙大蚜的感染数和健康虫数，计算感染率。每处理和每个处理后时间选取3棵柳树进行调查。 With the method of the present invention, apply on white willow, take the willow tree that has trunk and soil ant tunnel, do the following treatment respectively: (1) Utilize the method of the present invention to carry out the release block of aphid spores of aphids to control willow long beaked aphid; (2) Using the method of the present invention, place it again 10 days after the first placement of the Plagus spore release block; (3) Spray Plagiospores spores with a concentration of 10 8 /ml on the surface of the ant tunnel; ( 4) Open the ant tunnel and spray aphid spores with a concentration of 10 8 /ml; (5) Spray 5% imidacloprid wettable powder diluted 1000 times on the surface of the ant tunnel; (6) Open the ant tunnel and spray 1000 times diluted 5% imidacloprid wettable powder; (7) control, without any treatment. The ant tunnels were cut open 10 days and 20 days after the treatment, respectively, to investigate the number of infected and healthy insects in the ant tunnels, and to calculate the infection rate. Three willow trees were selected for each treatment and each post-treatment time for investigation.
研究结果见图1和2。结果表明，处理后10d，剖开蚁道喷洒蚜虫疠霉孢子的效果最好，柳长喙大蚜的感病可达66%，其次为蚂蚁携带蚜虫疠霉孢子的防治效果，柳长喙大蚜的感染率可达40%，对照组和在蚁道表面喷洒蚜虫疠霉孢子组，柳长喙大蚜均无感染。而通过二次放置蚜虫疠霉的孢子释放块10d后，隐匿于蚁道内的柳长喙大蚜的感染率可达70%以上，二次放置后20 d，柳长喙大蚜的感染率可达78%，控制效果显著，与剖开蚁道喷洒蚜虫疠霉孢子的效果无显著差异。 The results of the study are shown in Figures 1 and 2. The results showed that 10 days after the treatment, the effect of cutting the ant tunnel and spraying the spores of aphids Plasmodium spp. was the best, and the susceptibility of A. The infection rate of aphids can reach 40%. In the control group and the group sprayed with aphid spores on the surface of the ant tunnels, the willow beaked aphids were not infected. However, after placing the spore release block of P. aphidii for 10 days for the second time, the infection rate of A. aphids hiatus hidden in the ant tunnel can reach more than 70%. After 20 days after the second placement, the infection rate of A. Up to 78%, the control effect is significant, and there is no significant difference from the effect of spraying aphids Placis spores after opening ant tunnels.
剖开蚁道和在蚁道表面喷洒5%的吡虫啉1000×液进行柳长喙蚜的防治结果表明，在蚁道表面喷洒吡虫啉效果与对照相比无显著差异，而剖开蚁道后喷洒吡虫啉效果较好，蚜虫死亡率可达到80%。剖开蚁道喷洒吡虫啉后10d与放置蚜虫疠霉孢子释放块后20d对蚜虫的效果相比无显著差异（t=1.83, p=0.14），剖开蚁道喷洒吡虫啉后20d与放置蚜虫疠霉孢子释放块后30d对蚜虫的效果相比也无显著差异（t=1.99, p=0.12）。 The results of cutting open the ant tunnel and spraying 5% imidacloprid 1000× solution on the surface of the ant tunnel for the control of the willow aphid showed that there was no significant difference in the effect of spraying imidacloprid on the surface of the ant tunnel compared with the control, while spraying imidacloprid after cutting the ant tunnel The effect is better, and the mortality rate of aphids can reach 80%. There was no significant difference in the effect on aphids between spraying imidacloprid 10 days after opening the ant tunnel and 20 days after placing the P. There was no significant difference in the effect on aphids 30 days after the release of spores ( t =1.99, p =0.12).
以上研究结果表明，在柳长喙大蚜共生蚂蚁的蚁道口和蚁巢口放置我们发明的蚜虫疠霉孢子释放块，比剖开蚁道喷洒蚜虫疠霉孢子或吡虫啉更省时、省力，无需要任何专业知识即可完成操作，是一种更易于推广和应用的柳长喙大蚜防治方法。 The above research results show that it is more time-saving and labor-saving to place the release block of Plasmodium aphidoides spores or imidacloprid than cutting open the ant tunnels and spraying Plasmodium aphidida spores or imidacloprid at the ant tunnel opening and ant nest opening of the symbiotic ants of A. The operation can be completed without any professional knowledge, and it is a method of controlling the willow beak aphid that is easier to popularize and apply.
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