CN104126457A - Method for preventing and controlling trunk-hidden cinara tuiafulina by transmitting beauveria bassiana through ants - Google Patents
Method for preventing and controlling trunk-hidden cinara tuiafulina by transmitting beauveria bassiana through ants Download PDFInfo
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- 241000751139 Beauveria bassiana Species 0.000 title claims abstract description 79
- 241000257303 Hymenoptera Species 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 25
- 241000379617 Cinara Species 0.000 title 1
- 241000725475 Cinara fresai Species 0.000 claims abstract description 24
- 239000000843 powder Substances 0.000 claims abstract description 15
- 241000218691 Cupressaceae Species 0.000 claims description 49
- 241001124076 Aphididae Species 0.000 claims description 36
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 claims description 11
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 claims description 5
- 240000002495 Cucumis melo var. inodorus Species 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 10
- 208000015181 infectious disease Diseases 0.000 abstract description 10
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 210000002615 epidermis Anatomy 0.000 abstract 1
- 238000005507 spraying Methods 0.000 description 7
- 239000005906 Imidacloprid Substances 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- YWTYJOPNNQFBPC-UHFFFAOYSA-N imidacloprid Chemical compound [O-][N+](=O)\N=C1/NCCN1CC1=CC=C(Cl)N=C1 YWTYJOPNNQFBPC-UHFFFAOYSA-N 0.000 description 4
- 229940056881 imidacloprid Drugs 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 241001600407 Aphis <genus> Species 0.000 description 2
- 241000226709 Hesperocyparis arizonica Species 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 241000218636 Thuja Species 0.000 description 2
- 210000003323 beak Anatomy 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 239000004563 wettable powder Substances 0.000 description 2
- 208000032544 Cicatrix Diseases 0.000 description 1
- 241000223203 Coccidioides Species 0.000 description 1
- 241000255749 Coccinellidae Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000258923 Neuroptera Species 0.000 description 1
- 241001481659 Syrphidae Species 0.000 description 1
- 235000008109 Thuja occidentalis Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 230000037387 scars Effects 0.000 description 1
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- 239000004071 soot Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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Abstract
本发明公开了一种利用蚂蚁传播球孢白僵菌Beauveria bassiana防治树干隐匿柏长喙大蚜的方法，首先准备红糖猪油块，使红糖含量约为5-20%，凝结成块状后，切成0.5-2cm3见方的小块，然后使其在球孢白僵菌孢子粉中滚一下，做成孢子释放块。将其放置在蚁巢出口和树干蚁道入口处，供蚂蚁自由取食，蚂蚁取食过程中，会将球孢白僵菌孢子沾在表皮、口器和四肢上，当其与蚁道中隐匿的柏长喙大蚜接触时，将孢子传给柏长喙大蚜，柏长喙大蚜体表感染球孢白僵菌孢子后，感病死亡。本发明防治效果好，成本低，环保。
The invention discloses a method for using ants to spread Beauveria bassiana to prevent and control the hidden cypress aphid in tree trunks. Firstly, brown sugar lard blocks are prepared so that the brown sugar content is about 5-20%, and after they are condensed into blocks, Cut into 0.5-2cm 3 small pieces, then make it roll in the Beauveria bassiana spore powder to make spore release block. Place it at the exit of the ant nest and the entrance of the trunk ant tunnel for the ants to eat freely. During the feeding process of the ants, the spores of Beauveria bassiana will stick to the epidermis, mouthparts and limbs. When the cypress aphids come into contact with them, the spores are transmitted to the cypress aphids, and the cypress aphids are infected with the spores of Beauveria bassiana on the body surface, and die of infection. The invention has good control effect, low cost and environmental protection.
Description
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技术领域 technical field
本发明属于生物技术领域，涉及一种利用蚂蚁传播球孢白僵菌Beauveria bassiana防治树干隐匿柏长喙大蚜Cinara tuiafulina的方法。 The invention belongs to the field of biotechnology, and relates to a method for preventing and controlling the cypress aphid Cinara tuiafulina by using ants to spread Beauveria bassiana .
背景技术 Background technique
柏长喙大蚜是一种重要的林业害虫，主要危害侧柏、金钟柏、铅笔柏等，以成虫、若虫聚集幼茎表面吸食，常引起煤污病，影响柏树生长。部分虫体可寄生于柏树的树缝中或疤痕处，以口针刺吸到树干的韧皮部吸收柏树的营养，产生密露，蚂蚁吸食密露与柏长喙大蚜形成共生关系，蚂蚁一般在树缝的外部以泥土修筑的蚁道，保护蚜虫免受瓢虫、草蛉、食蚜蝇等天敌昆虫的捕食或病原体感染，因此防治难度很大。同时，隐匿型的柏长喙大蚜是其越冬的重要途径，冬天会转移到蚁巢内越冬，是来年蚜虫繁殖的重要因素，同时，也是柏长喙大蚜难以防治的重要原因之一。 Cypress long beaked aphid is an important forestry pest, which mainly harms arborvitae, thuja, and pencil cypress, and feeds on the surface of young stems with adults and nymphs. It often causes soot and affects the growth of cypress trees. Some worms can parasitize in the crevices or scars of cypress trees. They suck the phloem of the trunk with needles to absorb the nutrients of cypress trees and produce dense dew. Ant tunnels built with soil outside the tree gaps protect aphids from predation by natural enemies such as ladybugs, lacewings, and hoverflies or infection by pathogens, so it is very difficult to control. At the same time, the cryptic Aphid aphid is an important way for it to overwinter, and it will be transferred to the ant nest for overwintering in the winter, which is an important factor for the reproduction of aphids in the coming year, and it is also one of the important reasons why the aphid is difficult to control.
发明内容 Contents of the invention
本发明针对以上不足之处，提供了一种利用蚂蚁传播球孢白僵菌防治树干隐匿柏长喙大蚜的方法，通过与柏长喙大蚜共生的蚂蚁将球孢白僵菌传播给柏长喙大蚜，柏长喙大蚜体表感染球孢白僵菌孢子后，感病死亡，来达到对柏长喙大蚜的防治。 The present invention aims at the above deficiencies, and provides a method of using ants to spread Beauveria bassiana to prevent and cure the hidden cypress aphid, by spreading the Beauveria bassiana to cypress by symbiotic ants with the cypress aphid. Aphid cypress, Aphid cypress cypress, infected with Beauveria bassiana spores on the body surface, died of infection, so as to achieve the control of cypress cypress aphid.
本发明解决其技术问题所采用的技术方案是：一种利用蚂蚁传播球孢白僵菌防治树干隐匿柏长喙大蚜的方法，包含有如下步骤： The technical solution adopted by the present invention to solve its technical problems is: a method for using ants to spread Beauveria bassiana to prevent and treat the hidden cypress aphid in the trunk, comprising the following steps:
（1）准备球孢白僵菌孢子粉，可通过购买或固体发酵培养获得； (1) Prepare Beauveria bassiana spore powder, which can be obtained through purchase or solid fermentation culture;
（2）准备红糖猪油块 先将猪油融化，慢慢加入红糖融化，轻轻搅匀，使红糖含量占5-20%，冷却、凝结后做成红糖猪油块，将红糖猪油块切成0.5-2cm3见方的小块，冰箱内4℃冷藏备用； (2) To prepare the brown sugar lard block, first melt the lard, slowly add brown sugar to melt, stir gently, so that the brown sugar content accounts for 5-20%, cool and condense to make brown sugar lard block, brown sugar lard block Cut into small pieces of 0.5-2cm 3 squares, and refrigerate at 4°C for later use;
（3）将切成小块的红糖猪油块在球孢白僵菌孢子粉中滚动，使表面沾满孢子粉，做成球孢白僵菌孢子释放块； (3) Roll the brown sugar lard block cut into small pieces in the spore powder of Beauveria bassiana, so that the surface is covered with spore powder, and make the release block of Beauveria bassiana spores;
（4）将球孢白僵菌孢子释放块放置在树干有蚁道的柏树周围、与柏长喙大蚜共生的蚂蚁的蚁道口和蚁窝口，蚂蚁取食球孢白僵菌孢子释放块的过程中，将球孢白僵菌孢子沾在体表或口器或四肢上，蚂蚁在与蚁道内隐藏的柏长喙大蚜接触或取食蜜露时，将球孢白僵菌孢子传给柏长喙大蚜，柏长喙大蚜接触球孢白僵菌孢子后，感病死亡。 (4) Place the release block of Beauveria bassiana spores around the cypress tree with ant tunnel in the trunk, at the entrance of the ant tunnel and ant nest of the ants that live in symbiosis with Aphid cypress, and the ants eat the release block of Beauveria bassiana spores During the process, the spores of Beauveria bassiana are attached to the body surface or mouthparts or limbs, and when the ants contact with the cypress aphid hidden in the ant tunnel or eat honeydew, the spores of Beauveria bassiana are passed on. Give the cypress long-billed aphid, after the cypress long-billed aphid contacts the spores of Beauveria bassiana, it is infected and dies.
进一步的，第一次在树干有蚁道的柏树周围、与柏长喙大蚜共生的蚂蚁的蚁道口和蚁窝口，放置球孢白僵菌孢子释放块10天后，再放置一次球孢白僵菌孢子释放块，防治效果更好。 Further, for the first time around the cypress trees with ant tunnels in the trunk, at the ant tunnel openings and ant nest openings of ants that live in symbiosis with A. The fungus spore release block, the control effect is better.
本发明主要是利用了蚂蚁和柏长喙大蚜之间的共生关系，利用在外活动的蚂蚁将致病球孢白僵菌孢子传播给在树缝中隐匿的柏长喙大蚜，只需制作球孢白僵菌孢子释放块即可实现本发明，防治成本低，使用球孢白僵菌孢子引起柏长喙大蚜发病进行柏长喙大蚜的防治，不使用其他化学药剂，不破坏生态环境，环保； The present invention mainly utilizes the symbiotic relationship between ants and Aphid cypress, and uses the ants that move outside to spread the spores of Beauveria bassiana to the Aphid cypress hiding in the crevices of trees, and only needs to make The invention can be realized by releasing the spores of Beauveria bassiana, and the cost of prevention and control is low. Using the spores of Beauveria bassiana to cause the occurrence of the cypress aphid, the control of the cypress aphid does not use other chemicals and does not damage the ecology. environment, environmental protection;
实验证明，在树干有蚁道的柏树周围、与柏长喙大蚜共生的蚂蚁的蚁道口和蚁窝口，两次间隔放置球孢白僵菌孢子释放块20天后，柏长喙大蚜感染率在78%，防治效果与直接剖开树道喷洒球孢白僵菌孢子的效果一样好。本方法省时、省力，成本低廉，且事先无需调查蚁道内的蚜虫数量，更无需剖开蚁道对其中的柏长喙大蚜进行杀灭，只需在柏长喙大蚜共生蚁的蚁巢口或树干的蚁道口放置球孢白僵菌菌孢子释放块，就可达到防治柏长喙大蚜的目的，该方法不需要专业培训既可完成操作，并能达到同样的防治效果，有很强的可推广性，是一种有推广前景的柏长喙大蚜生物防治新方法。 Experiments have shown that around the cypress trees with ant tunnels in the trunk, at the ant tunnel entrance and the ant nest opening of ants that live in symbiosis with A. cypress, after 20 days of placing the spore release blocks of Beauveria bassiana at two intervals, the infection of A. cypress The rate was 78%, and the control effect was as good as that of directly cutting the tree path and spraying Beauveria bassiana spores. This method is time-saving, labor-saving and low in cost, and there is no need to investigate the number of aphids in the ant tunnel in advance, and it is not necessary to cut open the ant tunnel to kill the cypress aphid in it. Placing the spore release block of Beauveria bassiana coccidioides at the entrance of the nest or the ant passage of the trunk can achieve the purpose of preventing and controlling the cypress aphid. This method can complete the operation without professional training, and can achieve the same control effect. It is highly scalable and is a promising new biological control method for the cypress aphid.
附图说明 Description of drawings
图1所示为不同防治方法对树干蚁道内隐匿型柏长喙大蚜的控制效果； Figure 1 shows the control effects of different control methods on the hidden type Cypress aphid in the trunk ant tunnel;
图2 所示为吡虫啉对树干蚁道内隐匿型柏长喙大蚜的控制效果。 Figure 2 shows the control effect of imidacloprid on the occult cypress aphid in the trunk ant tunnel.
具体实施方式 Detailed ways
实施例1 Example 1
一种利用蚂蚁传播球孢白僵菌防治树干隐匿型柏长喙大蚜的方法，包含有如下步骤： A method of using ants to spread Beauveria bassiana to prevent and control the hidden type of cypress aphid, comprising the following steps:
（1）准备球孢白僵菌孢子粉，可通过购买或固体发酵培养获得； (1) Prepare Beauveria bassiana spore powder, which can be obtained through purchase or solid fermentation culture;
（2）准备红糖猪油块 先将猪油融化，慢慢加入红糖融化，轻轻搅匀，使红糖含量占5%，冷却、凝结后做成红糖猪油块，将红糖猪油块切成2cm3见方的小块，冰箱内4℃冷藏备用； (2) Prepare the brown sugar lard block first melt the lard, slowly add the brown sugar to melt, stir gently, so that the brown sugar content accounts for 5%, cool and condense to make brown sugar lard block, cut the brown sugar lard block into Small pieces of 2cm 3 square, refrigerated at 4°C for later use;
（3）将切成小块的红糖猪油块在球孢白僵菌孢子粉中滚动，使表面沾满孢子粉，做成球孢白僵菌孢子释放块； (3) Roll the brown sugar lard block cut into small pieces in the spore powder of Beauveria bassiana, so that the surface is covered with spore powder, and make the release block of Beauveria bassiana spores;
（4）将球孢白僵菌孢子释放块放置在树干有蚁道的柏树周围、与柏长喙大蚜共生的蚂蚁的蚁道口和蚁窝口，第一次在树干有蚁道的柏树周围、与柏长喙大蚜共生的蚂蚁的蚁道口和蚁窝口，放置球孢白僵菌孢子释放块10天后，再放置一次球孢白僵菌孢子释放块，蚂蚁会被红糖吸引进行取食，蚂蚁取食球孢白僵菌孢子释放块的过程中，将球孢白僵菌孢子沾在体表或口器或四肢上，蚂蚁在与蚁道内隐藏的柏长喙大蚜接触或取食蜜露时，将球孢白僵菌孢子传给柏长喙大蚜，柏长喙大蚜接触球孢白僵菌孢子后，感病死亡。 (4) The spore release block of Beauveria bassiana was placed around the cypress tree with ant tunnel in the trunk, at the ant tunnel opening and the ant nest opening of the ants that lived in symbiosis with Aphid cypress, for the first time around the cypress tree with ant tunnel in the trunk 1. Place the Beauveria bassiana spore release block for 10 days at the ant tunnel and ant nest openings of ants that live in symbiosis with Aphid cypress, and then place the Beauveria bassiana spore release block again, and the ants will be attracted by the brown sugar to feed , during the process of ants feeding on the release block of Beauveria bassiana spores, the spores of Beauveria bassiana stick to the body surface or mouthparts or limbs, and the ants contact or feed on the cypress aphid hidden in the ant tunnel During honeydew, the spores of Beauveria bassiana are transmitted to Aphid cypress, and the aphid dies after being exposed to spores of Beauveria bassiana.
实施例2 Example 2
一种利用蚂蚁传播球孢白僵菌防治树干隐匿型柏长喙大蚜的方法，包含有如下步骤： A method of using ants to spread Beauveria bassiana to prevent and control the hidden type of cypress aphid, comprising the following steps:
（1）准备球孢白僵菌孢子粉，可通过购买或固体发酵培养获得； (1) Prepare Beauveria bassiana spore powder, which can be obtained through purchase or solid fermentation culture;
（2）准备红糖猪油块 先将猪油融化，慢慢加入红糖融化，轻轻搅匀，使红糖含量占20%，冷却、凝结后做成红糖猪油块，将红糖猪油块切成0.5cm3见方的小块，冰箱内4℃冷藏备用； (2) Prepare the brown sugar lard block first melt the lard, slowly add brown sugar to melt, stir gently to make the brown sugar content account for 20%, cool and condense to make brown sugar lard block, cut the brown sugar lard block into Small pieces of 0.5cm 3 square, refrigerated at 4°C for later use;
（3）将切成小块的红糖猪油块在球孢白僵菌孢子粉中滚动，使表面沾满孢子粉，做成球孢白僵菌孢子释放块； (3) Roll the brown sugar lard block cut into small pieces in the spore powder of Beauveria bassiana, so that the surface is covered with spore powder, and make the release block of Beauveria bassiana spores;
（4）将球孢白僵菌孢子释放块放置在树干有蚁道的柏树周围、与柏长喙大蚜共生的蚂蚁的蚁道口和蚁窝口，第一次在树干有蚁道的柏树周围、与柏长喙大蚜共生的蚂蚁的蚁道口和蚁窝口，放置球孢白僵菌孢子释放块10天后，再放置一次球孢白僵菌孢子释放块，蚂蚁会被红糖吸引进行取食，蚂蚁取食球孢白僵菌孢子释放块的过程中，将球孢白僵菌孢子沾在体表或口器或四肢上，蚂蚁在与蚁道内隐藏的柏长喙大蚜接触或取食蜜露时，将球孢白僵菌孢子传给柏长喙大蚜，柏长喙大蚜接触球孢白僵菌孢子后，感病死亡。 (4) The spore release block of Beauveria bassiana was placed around the cypress tree with ant tunnel in the trunk, at the ant tunnel opening and the ant nest opening of the ants that lived in symbiosis with Aphid cypress, for the first time around the cypress tree with ant tunnel in the trunk 1. Place the Beauveria bassiana spore release block for 10 days at the ant tunnel and ant nest openings of ants that live in symbiosis with Aphid cypress, and then place the Beauveria bassiana spore release block again, and the ants will be attracted by the brown sugar to feed , during the process of ants feeding on the release block of Beauveria bassiana spores, the spores of Beauveria bassiana stick to the body surface or mouthparts or limbs, and the ants contact or feed on the cypress aphid hidden in the ant tunnel During honeydew, the spores of Beauveria bassiana are transmitted to Aphid cypress, and the aphid dies after being exposed to spores of Beauveria bassiana.
实施例3 Example 3
一种利用蚂蚁传播球孢白僵菌防治树干隐匿型柏长喙大蚜的方法，包含有如下步骤： A method of using ants to spread Beauveria bassiana to prevent and control the hidden type of cypress aphid, comprising the following steps:
（1）准备球孢白僵菌孢子粉，可通过购买或固体发酵培养获得； (1) Prepare Beauveria bassiana spore powder, which can be obtained through purchase or solid fermentation culture;
（2）准备红糖猪油块 先将猪油融化，慢慢加入红糖融化，轻轻搅匀，使红糖含量占12%，冷却、凝结后做成红糖猪油块，将红糖猪油块切成1cm3见方的小块，冰箱内4℃冷藏备用； (2) Prepare the brown sugar lard block first melt the lard, slowly add brown sugar to melt, stir gently to make the brown sugar content account for 12%, cool and condense to make brown sugar lard block, cut the brown sugar lard block into Small pieces of 1cm 3 square, refrigerated at 4°C for later use;
（3）将切成小块的红糖猪油块在球孢白僵菌孢子粉中滚动，使表面沾满孢子粉，做成球孢白僵菌孢子释放块； (3) Roll the brown sugar lard block cut into small pieces in the spore powder of Beauveria bassiana, so that the surface is covered with spore powder, and make the release block of Beauveria bassiana spores;
（4）将球孢白僵菌孢子释放块放置在树干有蚁道的柏树周围、与柏长喙大蚜共生的蚂蚁的蚁道口和蚁窝口，第一次在树干有蚁道的柏树周围、与柏长喙大蚜共生的蚂蚁的蚁道口和蚁窝口，放置球孢白僵菌孢子释放块10天后，再放置一次球孢白僵菌孢子释放块，蚂蚁会被红糖吸引进行取食，蚂蚁取食球孢白僵菌孢子释放块的过程中，将球孢白僵菌孢子沾在体表或口器或四肢上，蚂蚁在与蚁道内隐藏的柏长喙大蚜接触或取食蜜露时，将球孢白僵菌孢子传给柏长喙大蚜，柏长喙大蚜接触球孢白僵菌孢子后，感病死亡。 (4) The spore release block of Beauveria bassiana was placed around the cypress tree with ant tunnel in the trunk, at the ant tunnel opening and the ant nest opening of the ants that lived in symbiosis with Aphid cypress, for the first time around the cypress tree with ant tunnel in the trunk 1. Place the Beauveria bassiana spore release block for 10 days at the ant tunnel and ant nest openings of ants that live in symbiosis with Aphid cypress, and then place the Beauveria bassiana spore release block again, and the ants will be attracted by the brown sugar to feed , during the process of ants feeding on the release block of Beauveria bassiana spores, the spores of Beauveria bassiana stick to the body surface or mouthparts or limbs, and the ants contact or feed on the cypress aphid hidden in the ant tunnel During honeydew, the spores of Beauveria bassiana are transmitted to Aphid cypress, and the aphid dies after being exposed to spores of Beauveria bassiana.
应用实例： Applications:
以本发明的方法，在泰山柏树上进行应用，取有树干有泥土蚁道的柏树，分别做以下处理：（1）利用本发明方法通过蚂蚁携带球孢白僵菌孢子释放块防治柏长喙大蚜；（2）利用本发明的方法，分别在第一次放置球孢白僵菌孢子释放块后10 d 再次放置一次；（3）在蚁道表面喷洒浓度为108个/ml的球孢白僵菌孢子；（4）剖开蚁道喷洒浓度为108个/ml的球孢白僵菌孢子；（5）在蚁道表面喷洒稀释1000倍5%的吡虫啉可湿性粉剂；（6）剖开蚁道喷洒稀释1000倍5%的吡虫啉可湿性粉剂；（7）对照，不做任何处理。分别于处理后10 d和20d剖开蚁道进行调查蚁道内柏长喙大蚜的感染球孢白僵菌的数量或死亡虫数和健康虫数，计算感染率（死亡率）。每处理每个处理后时间选取3棵柏树进行调查。 With the method of the present invention, apply on Taishan cypress, get the cypress that has trunk to have earth ant tunnel, do following processing respectively: (1) utilize the method of the present invention to carry out the control of cypress long beak by ant carrying Beauveria bassiana spore release block large aphid; (2) using the method of the present invention, place it again 10 days after placing the spore release block of Beauveria bassiana for the first time; (3) spray balls with a concentration of 10 8 /ml on the surface of the ant tunnel Beauveria bassiana spores; (4) splitting the ant tunnel and spraying Beauveria bassiana spores at a concentration of 10 8 /ml; (5) Spraying 1000 times 5% imidacloprid wettable powder on the surface of the ant tunnel; (6 ) Open the ant tunnel and spray 5% imidacloprid wettable powder diluted 1000 times; (7) Control without any treatment. 10 days and 20 days after the treatment, the ant tunnels were cut open to investigate the number of Beauveria bassiana infected by Aphid cypress in the ant tunnels or the number of dead insects and healthy insects, and the infection rate (mortality rate) was calculated. Three cypress trees were selected for investigation after each treatment.
研究结果见图1和图2。结果表明，处理后10d，剖开蚁道喷洒球孢白僵菌孢子的效果最好，柏长喙大蚜的感病可达66%，其次为蚂蚁蚁携带球孢白僵菌孢子的防治效果，柏长喙大蚜的感染率可达40%，对照组和在蚁道表面喷洒球孢白僵菌孢子，柏长喙大蚜均无感染。而通过二次放置球孢白僵菌的孢子释放块10d后，隐匿于蚁道内的柏长喙大蚜的感染率可达48%，二次放置后20 d，柏长喙大蚜的感染率可达70%，控制效果显著，与蚁道喷洒球孢白僵菌孢子（感染率为69%）（t=0.34, p=0.74）或吡虫啉（死亡率为76%）（t=1.89, p=0.13）10d后的效果无显著差异。 The results of the study are shown in Figures 1 and 2. The results showed that 10 days after the treatment, the effect of spraying the spores of Beauveria bassiana after cutting the ant tunnel was the best, and the susceptibility of Aphid cypress was up to 66%, followed by the control effect of ants carrying Beauveria bassiana spores , the infection rate of Aphis cypresse can reach 40%. In the control group and spraying Beauveria bassiana spores on the surface of ant tunnels, Aphis cypresse had no infection. However, after placing the spore release block of Beauveria bassiana for the second time for 10 days, the infection rate of Aphid cypress hidden in the ant tunnel can reach 48%. After 20 days of second placement, the infection rate of A. It can reach 70%, and the control effect is significant. Compared with ant tunnel spraying Beauveria bassiana spores (infection rate is 69%) ( t =0.34, p =0.74) or imidacloprid (mortality rate is 76%) ( t =1.89, p =0.13) There was no significant difference in the effect after 10d.
但通过在柏长喙大蚜共生蚂蚁的蚁道口和蚁窝口放置我们发明的球孢白僵菌孢子释放块，比剖开蚁道喷洒孢子更省时、省力，无需要任何专业知识即可完成操作，是一种更易于推广和应用的柏长喙大蚜防治方法。 However, by placing the Beauveria bassiana spore release block invented by us at the entrance of the ant tunnel and ant nest of the symbiotic ant of the cypress beak, it is more time-saving and labor-saving than opening the ant tunnel and spraying the spores, and no professional knowledge is required. Completing the operation is a more easily popularized and applied control method for the cypress beaked aphid.
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