CN103937668B - Nostoc Algal Ecology simulation culture systems and cultural method - Google Patents

Abstract

The invention provides an ecological simulation culture system and culture method for algae of the genus Nostoc. The system includes a culture fluid recovery pool (7), a multi-layer culture rack (1), multiple lighting devices (3), and a recovery fluid filtration system (6) , at least one spray head (12). The method is to place the culture plate with holes on a multi-layer culture rack, and the algae species used for inoculation are the algae species with a diameter of 1 mm to 2 mm obtained through aseptic culture, and the algae species are evenly spread on the culture plate with holes when inoculating. , the inoculation density was controlled at 0.5? g/cm ² ~1g/cm ² , the inoculation thickness is 1cm-2cm; spray the culture solution on the culture, control the light and the temperature of the culture solution. This method can save water by 80%, and the spheres are not easy to break during the cultivation process of Gexianmi. During the cultivation process, Gexianmi has strong anti-algae pollution and low algae pollution rate. %.

Description

Nostoc algae ecological simulation culture system and culture method

technical field

The invention belongs to the technical field of algae cultivation, in particular to an ecological simulation cultivation system and a cultivation method of algae of the genus Nostoc, in particular to a cultivation system and a cultivation method of Gexianmi.

Background technique

Gexianmi is a kind of rare freshwater cyanobacteria that can be used for both medicine and food, which is a special product in Zouma Town, Hefeng, Hubei, my country. Because Ge Hongxian, an alchemist, medical scientist, and Taoist theorist known as Ge Xiangong in the Eastern Jin Dynasty, and the emperor and prince fell ill after eating this for a long time The body became smoother, so it was named "Ge Xianmi". It belongs to Cyanophyta, Cyanophyceae, Hormogonales, Nostocaceae, Nostoc ), the scientific name is "Nostocsphaeroidskuz". Gexianmi is a multicellular population, its main shape is spherical, and a few are spindle-shaped, elliptical, dumbbell-shaped or flat. There is a common colloidal sheath outside the filaments, which is easy to form a layered structure. It is relatively firm and dark green. The diameter of wild populations can reach 2 cm, and the diameter of artificially cultivated Gexianmi can reach 5 cm.

Gexianmi is a traditional freshwater algae used for both medicine and food in my country. Since the Eastern Jin Dynasty, many famous medical books and recipes have recorded a lot of Gexianmi. Medical books such as "Famous Doctors", "Compendium of Materia Medica", "Medicine Properties Test", "National Chinese Herbal Medicine Compilation" and other medical books believe that Gexianmi can improve eyesight, benefit qi, cure night blindness, relieve heat and clear partition, eliminate fatigue, treat burns and beautify and other effects; because of its beautiful shape, moist and delicious taste, and refreshing aroma, Gexianmi is listed in many ancient food book recipes such as "Green Vegetables", "Wild Cookbook", "Diaoding Collection", "Confucian Famous Cuisine" and so on. There are many cooking methods recorded, and the Qing history records it as a tribute and imperial meal. In "My First Half of Life" written by the last emperor Puyi, he has always been obsessed with "Duck Distilled Ge Xianmi". The "People's Daily" on March 2, 1980, the overseas edition of "People's Daily" on September 26, 1994, and the "Newspaper Digest" of Jiefang Daily on October 13, 1994 all introduced Ge Xianmi and called it "Rare Treasures".

Gexianmi has extremely high nutritional value. Modern experimental analysis results show that: Gexianmi contains 18 kinds of amino acids, including eight kinds of amino acids necessary for the human body; the content of various components in the dry matter of Gexianmi is about 56% of the total protein (10% of which is phycocyanin with anti-cancer and anti-cancer functions), total sugar is 24.19% (8-12% of which is active polysaccharides that can resist radiation and anti-cancer), crude fat 8.11%, carbohydrates 12.69%, Ash content 10.88%, chlorophyll 30.98mg/g; ascorbic acid 5.5mg/g, vitamin C content close to fresh jujube, more than 5 times higher than hawthorn; vitamin B1, B2 higher than general bacteria and algae; 15 kinds of minerals, the most abundant are Phosphorus, sulfur, calcium, potassium, iron, etc., less lead, silicon, magnesium, barium, germanium, etc., trace elements include zinc, copper, manganese, starch and sugar, and its calcium content is higher than that of ordinary vegetables , is an excellent natural calcium-rich nutritional food. Because Gexianmi has the functions of diet therapy, health care and nourishment, and its nutritional composition ratio is very suitable for the needs of the human body, it is recommended by the World Health Organization as "the best health food".

Because Gexianmi has special effects such as delicacy, diet therapy, health care, and nourishment, some merchants have vigorously promoted it in recent years, making Gexianmi more and more recognized by people, the market demand is increasing, and the price is also increasing. The price is getting higher and higher. The price of high-quality fresh products on the market is as high as 400 yuan/kg, and the price of dry products is as high as 3,600 yuan/kg.

According to academician Li Shanghao of the Institute of Hydrobiology, Chinese Academy of Sciences, Gexianmi has extremely strict requirements on the growing environment, and its growth range is very narrow. Except for more than 10,000 mu in Zouma Town, Hefeng County, Hubei Province, it is only distributed in a small amount in Africa. Due to its narrow natural distribution and low yield, it was mainly used as tribute and imperial meal for royals and dignitaries in the past. In recent years, with the extensive use of pesticides and chemical fertilizers, coupled with increasingly serious environmental pollution, the growing environment of Gexianmi has been greatly damaged, and the fields suitable for the growth of Gexianmi are becoming less and less, and the yield is getting lower and lower. Its natural The annual output is less than five tons, and the resources are on the verge of exhaustion.

In recent years, many researchers have invested a large amount of manpower and material resources to study the artificial cultivation method of Ge Xianmi. At present, the existing research reports on the artificial cultivation method of Ge Xianmi: disclose in Chinese patent literature CN1294190 (application date: 1999.10.22) The cultivation method includes the preparation of culture solution, preparation of algae species, aeration cultivation, hardening treatment, screening and harvesting and drying. The cultivation container is a glass tank or a raceway pool. The method disclosed in the Chinese patent document CN1528127 (application date: 2003.10.10) includes steps such as culture solution preparation, algal seed preparation, bioreactor aerated culture, hardening treatment, screening and harvesting. Chinese patent document CN101792716 (application date: 2010.03.10) adopts a semi-enclosed indoor pool to realize large-scale production, and discloses the structural principle of the pool and the optimization conditions for cultivating 7 basic elements of Ge Xianmi. Chinese patent document CN201424471 (application date: 2009.06.09) discloses an air lift device and an artificial culture device for Ge Xianmi cultivation, and the highly dispersed small air packets produced during the cultivation process are diffused in the culture solution , so that Gexianmi forms a regular rising and falling movement during the growth process. No matter the culture container used in the above patent documents in the cultivation process is a glass tank, a bioreactor, a water pool or a raceway pool, it is necessary to mix Ge Xianmi with the culture medium, and it is necessary to make the Ge Xianmi fermented by aeration or impellers during the whole cultivation process. The rice is suspended in the culture medium. In the nearly 90-day culture period, the culture medium needs to be replaced every 7 to 10 days. Before harvesting, it needs to be hardened to improve the mechanical strength of the sphere; A large amount of air suspends Gexianmi in the culture medium, and the severe impact force of the airflow and the friction with the culture container make the Gexianmi spheres vulnerable to damage and severe breakage. Generally, the breakage rate is between 5% and 10%, and the breakage is serious. More than 10%, even all of them will burst when the culture medium is replaced. Chinese patent document CN1544612 (application date: 2003.11.25) discloses a method for controlling pollution in the cultivation process of Gexianmi and ground fungus, which mainly includes purebred cultivation, disinfection of culture containers, manual cleaning of Gexianmi, filtration and replacement Culture medium and other steps. Since Gexianmi grows in aqueous solution, its water content is very high, and its dry matter content is generally only about 1.5-2%. In the above patents related to the cultivation of kudzu rice, the consumption of water, electricity and labor is large, and the production cost is high.

Contents of the invention

Aiming at the technical problems of high water and electricity consumption, easy breakage, easy to be polluted by miscellaneous algae, and low dry matter content of cultivated Gexianmi in the prior art, this application aims to provide an ecological algae of the genus Nostoc. Simulation culture system and culture method. The system is tailor-made according to the cultivation requirements of Gexianmi. It has a simple structure, can control the temperature of the culture medium, and recycle it, and is suitable for the cultivation of algae of the genus Nostoc. The cultivation method can save more than 80% of water and more than 50% of electricity consumption, so that the kudzu rice is not broken during the cultivation process, the pollution rate of miscellaneous algae is low, and the dry matter content of the kudzu rice is obviously increased.

For realizing above-mentioned purpose of the invention, technical scheme of the present invention is:

The Nostoc algae ecological simulation culture system includes a culture solution recovery tank, and also includes a multi-layer culture frame composed of a plurality of vertical support rods for supporting and a plurality of horizontal support rods for placing culture plates with holes, A plurality of illumination devices, a recovery fluid filtration system, a culture fluid delivery controller, a culture fluid delivery pipeline, and at least one nozzle; The bottom of the rack is provided with a culture solution recovery tray, which is connected to the culture solution recovery tank through the recovery pipeline and the recovery solution filtration system; the culture solution recovery tank is connected with a culture solution delivery pump and temperature control equipment; The culture fluid delivery pump communicates with the nozzle through the culture fluid delivery pipeline, and the nozzle is located above the top layer of the multi-layer culture rack.

The culture fluid delivery pipeline is preferably provided with a culture fluid delivery controller.

The hole diameter of the culture plate with holes is preferably ≤1mm.

The layer spacing of the multi-layer culture rack is preferably 30cm-40cm.

The distance between the spray head and the top layer of the multi-layer culture rack is preferably 40cm-50cm.

Nostoc algae ecological simulation culture method, using the culture system, the specific steps are:

(1) Take the glass greenhouse or plastic greenhouse as the cultivation site, place the above-mentioned cultivation system in the cultivation site, and place the culture plate with holes on the multi-layer culture rack. The interval between each layer of the multi-layer culture rack is 30cm-40cm. The hole diameter of the well culture plate is ≤1mm, and the distance between the nozzle and the top layer of the multi-layer culture rack is set to 40cm-50cm;

(2) The algae species used for inoculation are algae species with a diameter of 1 mm to 2 mm obtained through aseptic culture. When inoculating, the algae species are evenly spread on the culture plate with holes, and the inoculation density is controlled at 0.5 g/cm ² to 1 g /cm ² , the inoculation thickness is 1cm-2cm;

(3) Spray the culture solution on the culture, the spray interval time is 10-30 minutes, and the duration of each spray is 1-2 minutes. The time adjustment is based on keeping the surface of all cultures moist. The lower layer drips, and finally flows to the culture fluid collection tray at the bottom, and then is recycled to the culture fluid recovery pool for recycling; at the same time, the light is controlled to be kept between 1000lx and 20000lx, and the temperature of the culture fluid is controlled to be kept between 20°C and 30°C between;

Described nutrient solution composition and concentration are:

The algae of the genus Nostoc is Gexianmi or ground fungus.

The present invention is further explained and illustrated below:

The basis of the present invention is: through the in-depth understanding of the growth conditions of wild Gexianmi in nature and artificial cultivation of Gexianmi in the laboratory, we simulate the natural growth mode of Gexianmi and combine modern biotechnology to change the quality of Gexianmi. In the cultivation mode, the average daily fresh weight gain can reach more than 5% during the cultivation process. Since the current research on the ecological simulation production system of Gexianmi has just started, there is still a great potential to increase its yield.

Cultivation of Gexianmi includes the following steps: composition and preparation of culture solution, control of light and temperature conditions, cultivation of algae species, inoculation, cultivation, recovery and treatment of culture solution, harvest and storage.

A. Use glass greenhouse or plastic greenhouse as the cultivation site to make full use of natural light and temperature resources; at the same time, set up a sunshade net that can be automatically switched on and off on the top of the greenhouse, and add a certain amount of artificial lighting equipment in the greenhouse to keep the indoor light at 1000-20000lx; through the ground Heat source air conditioners and other temperature control equipment directly control the temperature of the culture medium, and by controlling the interval of spraying the culture, the temperature of the culture is kept at 20-30°C, effectively reducing energy consumption.

B. The frame of the training frame is made of aluminum alloy, and the interval between each layer is 30-40cm. According to the light characteristics of different seasons, the distance between layers is adjusted to keep the light intensity in a suitable range. A culture fluid collection tray is placed at the bottom of the culture rack and connected to the culture fluid recovery pipeline to recover and filter the culture fluid. The frame of the culture plate with holes is made of stainless steel or other non-toxic materials, and the bottom is a stainless steel mesh with a hole diameter of 1mm or a non-toxic organic material mesh. Spray the culture medium at intervals to keep the culture at proper humidity and temperature.

C. The basic composition and concentration of the culture medium are the same as above. The formula of the culture medium needs to be adjusted accordingly in different growth stages of Gexianmi, and the recovered culture medium needs to be supplemented according to the loss of nutrients when it is recycled.

D. The algae species used for inoculation should be excellent algae species with a diameter of 1-2 mm obtained through aseptic culture. When inoculating, the algae species should be evenly spread on the culture plate with holes, and the inoculation density should be controlled at 0.5-1 g/cm ² , according to the season and light intensity to make certain adjustments.

E. The automatic controller controls the nozzle installed on the top of the culture rack to spray the culture solution to the culture, the spray interval is 10-30 minutes, and the duration of each spray is 1-2 minutes, and the time is adjusted to maintain the surface of all cultures Wet is standard. The culture fluid drips from the upper layer to the lower layer, and finally flows to the culture fluid collection tray at the bottom, and is then recycled to the culture fluid recovery system.

F. The recovered culture solution needs to filter out impurities and flow into the recovery tank, and then control the temperature of the culture solution at 20-25°C through the temperature control system in the recovery tank, and then reuse it through the spraying equipment.

The ecological simulation production method and culture system of Ge Xianmi are also suitable for the large-scale production of other algae such as the fungus Nostoc.

When the Gexianmi ecological simulation culture system provided by the present invention is used for large-scale cultivation of Gexianmi, it is only necessary to put the Gexianmi algae species into a belt made of a stainless steel frame with a pore diameter of about 1mm at the bottom. Well culture plate, cultured on the culture rack.

In recent years, many researchers have invested a lot of manpower and material resources to study the artificial cultivation methods of Ge Xianmi. At present, all artificial cultivation methods related to Ge Xianmi include culture solution preparation, algae preparation, aeration culture, hardening treatment, screening and harvesting. And drying and other links, the culture container is a glass tank or a runway pool. These methods all have the problems of many production links, large water consumption, high energy consumption, easy rupture of the sphere, low dry matter content and high production cost. Therefore, we have designed a brand-new Ge Xianmi ecological simulation cultivation system and method, which can effectively solve these problems.

No matter the culture container used is a glass tank, bioreactor, water pool or raceway pool, it is necessary to mix Ge Xianmi with the culture medium. During the whole cultivation process, the Ge Xianmi must be suspended in the culture liquid through ventilation or impellers. In the nearly 90-day culture period, the culture medium needs to be replaced every 7-10 days, and hardening treatment is needed to improve the mechanical strength of the spheres before harvesting. In the new culture system, we simplify the steps and remove the process of aeration, cleaning, changing culture medium, hardening, etc., which can save a lot of manpower, effectively improve work efficiency and reduce production costs.

In the new culture system, we put Gexianmi in the culture tray instead of suspending cultivation of Gexianmi, so we can save more than 80% of water; the temperature control is not carried out by controlling the entire cultivation space, but It is only necessary to control the temperature of the culture medium to keep the culture at a temperature suitable for growth, thus saving more than 50% of energy consumption.

In the new culture system, it can effectively inhibit the pollution of algae, and it can reduce the pollution by more than 70% compared with the traditional method.

The rupture of Gexianmi spheres has always been a difficult problem to solve during the cultivation process. This is mainly due to the need to feed a large amount of air to suspend Gexianmi in the culture medium during the cultivation process, and the severe impact force of the air flow and the friction with the culture container make the Gexianmi spheres vulnerable to damage and severe breakage. Between 5% and 10%, the brokenness is more than 10%, and it may even completely rupture when the culture medium is replaced. However, in the process of culturing with this method, the spheres of Gexianmi seldom break, and the breakage rate is close to zero.

In the traditional culture method, because Gexianmi grows in aqueous solution, its water content is very high, and its dry matter content is generally only about 1.5-2%. However, the dry matter content of Gexianmi cultivated by the method can be as high as 8%, which is 2-5 times that of the traditional method. Due to the high dry matter content and thus high mechanical strength, no special hardening process is required during cultivation.

Compared with the prior art, the present invention has the advantages of:

1. Gexianmi ecological simulation production system requires simple technology and equipment, small investment, low production cost and continuous production throughout the year;

2. When using this method, the light and temperature adaptability range of Ge Xianmi is wide, the temperature is 4-35 ℃, and the light can grow at 600-12000lx, and the photosynthesis efficiency is high;

3. The dry matter content of Gexianmi is 2 to 3 times higher than that of the suspension in the culture medium, and the dry matter content can reach 7 to 8%, which is 2 to 5 times that of other cultivation methods;

4. The pollution rate of kudzu rice is low, the resistance is good, and the sphere does not break;

5. Since only the temperature of the culture medium is controlled, energy consumption can be effectively reduced, saving energy by more than 50%;

6. More than 80% water can be saved during the cultivation process;

7. The composition and pH of the culture medium are close to the natural water body, which can be recycled and discharged directly without polluting the environment.

Description of drawings

Fig. 1 schematic diagram of the structure of the culture system of the present invention;

Fig. 2 enlarged structure schematic diagram of culture plate with holes described in the present invention;

Among them, 1 is a multi-layer culture frame, 2 is a culture plate with holes, 3 is a lighting device, 4 is a culture liquid recovery plate, 5 is a recovery pipe, 6 is a recovery liquid filtration system, 7 is a culture liquid recovery tank, and 8 is a culture medium. Liquid delivery pump, 9 is a temperature control device, 10 is a culture solution delivery controller, 11 is a culture solution delivery pipeline, 12 is a nozzle, 13 is a vertical support rod, and 14 is a horizontal support rod.

detailed description

Below in conjunction with embodiment the present invention will be further described.

Example 1

The Nostoc algae ecological simulation culture system includes a culture solution recovery tank 7, and also includes a plurality of vertical support rods 13 for supporting and a plurality of horizontal support rods 14 for placing the culture tray 2 with holes. Layer culture frame 1, multiple lighting devices 3, recovered liquid filtration system 6, culture liquid delivery controller 10, culture liquid delivery pipeline 11, at least one nozzle 12; Underneath the layer horizontal support bar 14, the bottom of the multi-layer culture frame 1 is provided with a culture solution recovery tray 4, and the culture solution recovery tray 4 communicates with the culture solution recovery pool 7 through the recovery pipeline 5 and the recovery solution filtration system 6; The culture fluid recovery pool 7 is connected with a culture fluid delivery pump 8 and a temperature control device 9; the culture fluid delivery pump 8 communicates with the nozzle 12 through the culture fluid delivery pipeline 11, and the nozzle 12 is located in the multi-layer culture rack 1 above the top.

A culture solution delivery controller 10 is provided on the culture solution delivery pipeline 11 . The perforated culture tray 2 is a mesh stainless steel body with a pore diameter of ≤1 mm. The layer spacing of the multi-layer culture frame 1 is 30cm-40cm. The distance between the spray nozzle 12 and the top layer of the multi-layer culture frame 1 is 40cm-50cm.

Example 2

Taking Gexianmi as an example, the method for cultivating Gexianmi ecological simulation is cultivated with the cultivation system described in Example 1, which specifically includes:

Cultivation site and equipment: use glass greenhouse or plastic greenhouse as the training site, place the culture system in the training site, place the culture plate with holes on the multi-layer culture rack, and the interval between each layer of the multi-layer culture rack is 30cm～ 40cm, the hole diameter of the culture plate with holes is ≤1mm, and the distance between the nozzle and the top layer of the multi-layer culture rack is 40cm-50cm;

Light source: The light intensity required for the growth of Gexianmi is 1000-8000lx. When the natural light intensity meets this condition, artificial light sources can be used. When the light intensity is too high, the light intensity needs to be reduced through the sunshade net. Commonly used light sources are waterproof fluorescent tubes or LED lights, and these lights are respectively arranged under the horizontal frame of the cultivation frame to maintain the algae body to obtain 1000-8000lx light intensity.

Temperature: The growth temperature range of Gexianmi is 10-35°C, and the optimum temperature is around 20-25°C. When the natural temperature is within this temperature range, you only need to open the side membrane and top membrane of the greenhouse for ventilation; when the temperature is too low or too high, you need to adjust the temperature of the culture medium and the interval of spraying to keep the culture suitable. temperature.

The composition and concentration of the culture medium are:

Preparation of algae seeds: There are two types of algae species: first-class and second-class. When preparing the first-grade algae species, first prepare the culture solution with distilled water, then divide it into 50-1000ml glass triangular flasks or reagent bottles, seal it, sterilize it at 121°C for 20 minutes, and cool it for later use; then select the vigorous growth and good shape. , good elasticity and pollution-free Gexianmi spheres, sterilized and homogenized in the laboratory under strict aseptic conditions, inoculated into the prepared medium, and filtered air was introduced for aeration culture; the temperature during culture was controlled at 20 -25°C, the light is controlled at 3000-5000lx, and an appropriate amount of red light is added. When the algal body is cultivated to a diameter of 0.5-1mm, transfer it to a 10-20L large glass bottle for expanded cultivation, and the culture conditions are the same as above; when the algae body grows to a diameter of about 2mm, check that the growth state is good and there is no pollution. secondary algae production species).

Inoculation and cultivation: Put the high-quality production seeds prepared by the above method into a culture plate with a hole in the bottom of 1mm, control the inoculation density at 0.5g/cm ² ~1g/cm ² , and inoculation thickness of 1-2cm, and then inoculate Place the culture plate with holes in layers on the culture rack, control the temperature of the culture medium at 20-25°C, spray the culture medium into the plate at a certain interval, and keep the culture surface moist and maintain the temperature of the culture at intervals. The temperature is 15-30°C; when the light is too strong or insufficient, the light should be supplemented by shading or artificial light to make it meet the cultivation conditions. The sprayed culture solution leaks from the upper layer to the lower layer layer by layer, and finally falls into the bottom layer of the culture solution collection tray, and then flows into the collection pipe through the outlet at the bottom of the collection tray, enters the culture solution collection pool after being filtered by the filter equipment, and then passes through the temperature control The equipment adjusts the temperature of the culture medium to 20-25°C (slightly higher in winter and slightly lower in summer), and then reuses it through the spraying pipeline. The concentration of the culture solution components in the collection pool must be monitored, and the lost part must be replenished in time. During the cultivation process, it is necessary to turn the Gexianmi 1-2 times a day, and when the growth of Gexianmi doubles, divide it into two culture plates with holes to continue cultivating, and so on until the Gexianmi grows to 6-8mm. Sieve and classify and sell directly or after drying in the shade.

Product testing: It was observed by naked eyes that the spheres of Gexianmi did not break, which effectively inhibited the pollution of algae, and compared with the traditional method, the pollution was reduced by more than 70%. After drying, the dry matter content of Gexianmi was found to be 7%- 8%.

Claims (3)

1. Nostoc Algal Ecology simulation culture systems, comprise nutrient solution pond for recovering (7), it is characterized in that, also comprising Multilayer culture shelf (1), multiple illumination apparatus (3), Ethylene recov filtering system (6), nutrient solution delivery controller (10), nutrient solution transport pipe (11), at least one shower nozzle (12) of being made up of multiple passive vertical support bar (13) and multiple horizontal support bar (14) for placing culture plate with holes (2); Described illumination apparatus (3) is arranged on the below of any one horizontal support bar (14) of Multilayer culture shelf (1), described Multilayer culture shelf (1) bottom is provided with nutrient solution recovering tray (4), and described nutrient solution recovering tray (4) is communicated with nutrient solution pond for recovering (7) by recovery channel (5), Ethylene recov filtering system (6); Described nutrient solution pond for recovering (7) is provided with nutrient solution transferpump (8) and temperature control device (9) in being communicated with; Described nutrient solution transferpump (8) is communicated with shower nozzle (12) by nutrient solution transport pipe (11), and described shower nozzle (12) is positioned at the top of Multilayer culture shelf (1) top layer; Aperture≤the 1mm of described culture plate with holes (2), and the aperture of described culture plate with holes (2) is less than the diameter of nostoc sphaeroides; The interlamellar spacing of described Multilayer culture shelf (1) is 30cm-40cm, and described shower nozzle (12) is 40cm-50cm with the distance of Multilayer culture shelf (1) top layer; Described Nostoc algae is Nostoc.

2. Nostoc Algal Ecology simulates culture systems according to claim 1, and it is characterized in that, described nutrient solution transport pipe (11) is provided with nutrient solution delivery controller (10).

3. Nostoc Algal Ecology simulation cultural method, is characterized in that, use culture systems described in claim 1 or 2, concrete steps are:

(1) with glasshouse or plastic greenhouse greenhouse for cultivating place, described for one of claim 1-2 culture systems is placed in and cultivates in place, culture plate with holes is placed on Multilayer culture shelf, the every interlayer of Multilayer culture shelf is divided into 30cm ~ 40cm, culture plate aperture≤1mm with holes, the distance arranging shower nozzle and Multilayer culture shelf top layer is 40cm-50cm;

(2) the inoculation algae kind of algae kind to be the diameter obtained by sterile culture be 1mm ~ 2mm, be evenly laid in culture plate with holes by algae kind during inoculation, the density domination of inoculation is at 0.5g/cm ²~ 1g/cm ², inoculation thickness is 1cm-2cm;

(3) on culture, nutrient solution is sprayed, spray 10 ~ 30 minutes interval times, 1 ~ 2 minute each spray time length, the adjustment of time is to keep all culture surface wettabilities for standard, nutrient solution is dripped to lower floor by upper strata, finally flow to the nutrient solution catch tray of the lowest layer, then be recovered to nutrient solution pond for recovering, recycle; Meanwhile, controlled light remains between 1000lx ~ 20000lx, controls culture-liquid temp and remains between 20 DEG C ~ 30 DEG C;

Described nutrient solution composition and concentration are: