CN103865793B - Nostoc algae shallow-layer culture systems and cultural method - Google Patents

Abstract

The invention provides a shallow culture system and a culture method for algae of the genus Nostoc. The system includes a culture solution recovery pool (7), a multi-layer culture frame (1), multiple lighting devices (3), and a recovery solution filtration system (6) , at least one drip opening (12). The method is to place the shallow culture plate on a multi-layer culture frame, and the algae species used for inoculation are the algae species with a diameter of 1 mm to 2 mm obtained through aseptic culture, and the algae species are evenly spread on the shallow culture plate when inoculating. , the inoculation density is controlled at 0.5g/cm ² ~1g/cm ² , the inoculation thickness is 1cm-2cm; the culture solution is sprayed on the culture, and the light and the temperature of the culture solution are controlled. The system does not require a special air pump to increase oxygen and carbon dioxide. This cultivation method simplifies the steps and eliminates the process of aeration, cleaning, and changing the culture medium. It can save more than 60% of water and more than 50% of electricity, and can effectively remove miscellaneous algae Pollution, and Gexianmi is not easy to break.

Description

Nostoc algae shallow culture system and culture method

technical field

The invention belongs to the field of biotechnology, in particular to a shallow culture system and a culture method for algae of the genus Nostoc.

Background technique

The scientific name of Gexianmi is " Nostoc sphaeroids kuz ", Gexianmi is a multicellular group, the shape is mainly spherical, and there is a common colloidal sheath on the outside of its filaments, which is dark green. Groups can reach 5 cm in diameter. Medical books such as "Famous Doctors", "Compendium of Materia Medica", "Medicine Properties Test", "National Chinese Herbal Medicine Compilation" and other medical books believe that Ge Xianmi can improve eyesight, nourish qi, cure night blindness, relieve heat and clear partition, eliminate fatigue, treat burns and beautify Because of its mild medicinal properties, long-term consumption has the effects of health care and beauty.

The nutritional value of Ge Xianmi is extremely high, containing 18 kinds of amino acids, of which the eight essential amino acids are rich in content; the total protein content in the dry matter of Ge Xianmi is as high as about 56%, of which 10% are algae with anti-cancer and anti-cancer functions Blue protein; the content of active polysaccharides that can improve human immunity reaches 12%; polyunsaturated crude fatty acid is more than 10%; chlorophyll 30.98 mg/g; ascorbic acid 5.5 mg/g, vitamin C content is close to fresh jujube, 5 times higher than hawthorn More vitamins B1 and B2 than common bacteria and algae; 15 kinds of minerals are contained, and its calcium content is higher than that of ordinary vegetables. It is an excellent natural calcium-rich nutritious food. Because Gexianmi has the functions of diet therapy, health care and nourishment, and its nutritional composition ratio is very suitable for the needs of the human body, it is recommended by the World Health Organization as "the best health food".

According to academician Li Shanghao of the Institute of Hydrobiology, Chinese Academy of Sciences, Gexianmi has extremely strict requirements on the growing environment, and its growth range is very narrow. Except for more than 10,000 mu in Zouma Town, Hefeng County, Hubei Province, it is only distributed in a small amount in Africa. Due to its narrow natural distribution and low yield, it was mainly used as tribute and imperial meal for royals and dignitaries in the past. In recent years, with the extensive use of pesticides and chemical fertilizers, coupled with increasingly serious environmental pollution, the growing environment of Gexianmi has been greatly damaged, and the fields suitable for the growth of Gexianmi are becoming less and less, and the yield is getting lower and lower. Its natural The annual output is less than five tons, and the resources are on the verge of exhaustion. However, due to its delicacy, food therapy, health care, nourishing and other special effects, the market demand is increasing, causing the contradiction between supply and demand to become increasingly prominent.

There are existing research reports on the artificial cultivation method of Gexianmi: the cultivation method disclosed in Chinese patent document CN1294190 (application date: 1999.10.22) comprises culture solution formula and preparation, preparation of algae species, aeration cultivation, screening and grading, enhancing mechanical strength, harvesting and drying. The method disclosed in the Chinese patent document CN1528127 (application date: 2003.10.10) includes steps such as culture solution preparation, algal seed preparation, bioreactor aerated culture, hardening treatment, screening and harvesting. Chinese patent document CN101792716 (application date: 2010.03.10) adopts a semi-enclosed indoor pool to realize large-scale production, and discloses the structural principle of the pool and the optimization conditions for cultivating 7 basic elements of Ge Xianmi. Chinese patent document CN201424471 (application date: 2009.06.09) discloses an air lift device and an artificial culture device for Ge Xianmi cultivation, and the highly dispersed small air packets produced during the cultivation process are diffused in the culture solution , so that Gexianmi forms a regular rising and falling movement during the growth process.

No matter the culture container used in the cultivation process of the above patent documents is a glass jar, a bioreactor, a pool or a raceway pond, it is necessary to mix the Gexianmi with the culture solution, and it must be ventilated by an air pump or stirred by an impeller during the whole cultivation process. Gexianmi is suspended in the culture medium, and CO ₂ and O ₂ are provided for Gexianmi. During the nearly 90-day cultivation period, the algal bodies need to be cleaned and the culture medium replaced every 7 to 10 days; In the liquid process, the breakage of Gexianmi spheres is a very common problem. The general breakage rate is between 5% and 10%, and the breakage is more than 10% seriously. Especially in the case of large air flow or violent stirring, it may even cause All the Ge Xianmi spheres disintegrated. In addition, the general culture method consumes a lot of water and electricity, and the cost is high.

Contents of the invention

Aiming at the problems of high water and electricity consumption, easy breakage, easy contamination by miscellaneous algae, and high labor cost in the cultivation process of Gexianmi in the prior art, the present application aims to provide a shallow cultivation system and cultivation method for algae of the genus Nostoc. The system is tailor-made according to the cultivation requirements of Gexianmi. It has a simple structure and can directly control the temperature of the culture medium and recycle it. It does not need a special air pump to increase oxygen and carbon dioxide. It is suitable for the cultivation of Nostoc algae. The cultivation method can save more than 60% of water and more than 50% of electricity consumption, can effectively remove the pollution of miscellaneous algae, and the gexianmi is not easy to break during the cultivation process.

For realizing above-mentioned purpose of the invention, technical scheme of the present invention is:

The shallow culture system of Nostoc algae includes a culture solution recovery tank, and also includes a multi-layer culture frame composed of a plurality of vertical support rods for supporting and a plurality of horizontal support rods for placing shallow culture trays, A plurality of illumination devices, a recovery fluid filtration system, a culture fluid delivery controller, a culture fluid delivery pipeline, and at least one drip port; A culture fluid recovery tray is provided at the bottom of the culture frame, and the culture fluid recovery tray communicates with the culture fluid recovery tank through a recovery pipeline and a recovery fluid filtration system; the culture fluid recovery tank is connected with a culture fluid delivery pump and temperature control equipment; The culture fluid delivery pump is communicated with the drip port through the culture fluid delivery pipeline, and the drip port is located above the top layer of the multi-layer culture rack; the bottom of the shallow culture tray is provided with a plurality of holes, each of which is A sealing plug is provided, and a height-adjustable liquid level control dropper is arranged in the middle of the sealing plug.

A culture solution delivery controller is preferably provided on the culture solution delivery pipeline.

The aperture at the bottom of the shallow culture dish is preferably 0.8-1.2 cm.

The diameter of the liquid level control dropper is preferably 1mm-2 mm, and the length of the pipe is preferably 4cm-8cm, and the diameter of the liquid level control dropper is smaller than the diameter of the cultured algae.

The interlayer spacing of described multi-layer cultivation frame is preferably 30 cm-40cm, and interlayer spacing can be adjusted.

The distance between the drip opening and the top layer of the multi-layer culture rack is preferably 40cm-50cm.

Nostoc algae shallow culture method, using the culture system, the specific steps are:

(1) Take the glass greenhouse or plastic greenhouse as the cultivation site, place the culture system in the cultivation site, place the shallow culture tray on the multi-layer culture rack, and the interval between each layer of the multi-layer culture rack is 30 cm to 40 cm , the distance between the drip opening and the top layer of the multi-layer culture rack is set to be 40cm-50cm; the bottom of the shallow culture plate is provided with a plurality of holes, and the aperture of the shallow culture plate is 0.8-1.2 cm. plug, a height-adjustable liquid level control drip tube is arranged in the middle of the sealing plug;

(2) The algae species used for inoculation are algae species with a diameter of 1 mm to 2 mm obtained through aseptic culture. When inoculating, the algae species are evenly spread on a shallow culture plate, and the inoculation density is controlled at 0.5 g/cm ² to 1 g /cm ² , the inoculation thickness is 1 cm -2cm;

(3) According to the diameter of the culture, select a liquid level control dropper with a diameter smaller than that of Ge Xianmi particles, adjust the liquid level on the culture plate to control the height of the dropper, so that the liquid level of the culture solution poured into the culture plate is just submerged For the culture, the excess culture solution flows into the lower culture plate through the liquid level control dropper, and the culture solution is automatically dissolved into the fresh air when it drips into the culture plate; at the same time, the control light is kept between 1000lx and 20000lx, and the temperature of the culture solution is kept at Between 20°C and 30°C; the composition and concentration of the culture solution are:

MgSO ₄ . 7H ₂ O 0.05-0. 15g/L

K ₂ HPO ₄ . 3H ₂ O 0.005-0.08g/L

CaCl ₂ . 2H ₂ O 0.004-0.06g/L

EDTA-Na ₂ 0.001-0.004g/ L

Ammonium ferric citrate 0.001-0.005 g/ L

H ₃ BO ₄ 0.002-0.008mg/L

Na ₂ MoO ₄ .2H ₂ O 0.001-0.030mg/L

ZnSO ₄ .7H ₂ O 0.001-0.02mg/L

CuSO ₄ .5H ₂ O 0.001-0.005mg/L

MnCl ₂ .4H ₂ O 0.020-0.060 mg/L.

The algae of the genus Nostoc is Gexianmi or ground fungus.

Below the present invention is further explained and illustrated:

Cultivation of Gexianmi includes the following steps: composition and preparation of culture solution, control of light and temperature conditions, cultivation of algae species, inoculation, cultivation, recovery and treatment of culture solution, harvest and storage.

A. Use glass greenhouse or plastic greenhouse as the cultivation site, make full use of natural light and temperature resources; at the same time, set up a sunshade net that can be automatically switched on and off on the top of the greenhouse, and add a certain amount of artificial lighting equipment in the greenhouse to keep the indoor light at 1000-20000lx; through the ground Temperature control equipment such as heat source air conditioners directly control the temperature of the culture medium, and through the speed of dropping the culture medium, the temperature of the culture is kept at 20-30°C, effectively reducing energy consumption.

B． The frame of the training frame is made of aluminum alloy, and the interval between each layer is 30-40cm. According to the light characteristics of different seasons, the distance between layers is adjusted to keep the light intensity in a suitable range. A culture fluid collection tray is placed at the bottom of the culture rack and connected to the culture fluid recovery pipeline to recover and filter the culture fluid. The shallow culture plate is made of non-toxic and transparent materials, and the diameter of the glass tube is 1-4mm. After placing the Amygdalina species in the shallow culture plate, they are arranged in layers on the culture rack, and the flow rate of the culture medium is adjusted according to the need to keep the culture Suitable temperature.

C. The basic composition and concentration of the culture medium are the same as above. The formula of the culture medium needs to be adjusted accordingly in different growth stages of Gexianmi, and the recovered culture medium needs to be supplemented according to the loss of nutrients when it is recycled.

D. The algae species used for inoculation should be fine algae species with a diameter of 1-2 mm obtained through aseptic culture. When inoculating, the algae species should be evenly spread on the shallow culture plate, and the inoculation density should be controlled at 0.2-1g/cm ² , according to the season and light intensity to make certain adjustments.

E． The dripper installed on the top of the culture rack is controlled by the automatic controller to continuously drip the culture solution to the culture, and the flow rate of the culture solution is adjusted to keep the temperature of the culture solution in the culture tray at 20-25°C. The culture fluid drips from the upper layer to the lower layer, and finally flows to the culture fluid collection tray at the bottom, and is then recycled to the culture fluid recovery system. According to the diameter of the culture, select a glass tube with a diameter slightly smaller than the diameter of Gexianmi particles, adjust the height of the glass tube on the culture plate, so that the liquid level of the culture liquid poured into the culture plate just submerges the culture, and the excess culture liquid passes through the glass tube It flows into the lower culture plate, and the culture solution can automatically dissolve into fresh air when it drips into the culture plate.

F． The recovered culture solution needs to filter out impurities and flow into the recovery tank, and then control the temperature of the culture solution at 20-25°C through the temperature control system in the recovery tank, and then reuse it through the spraying equipment.

G. When the cultured Gexianmi grows too densely, it should be divided into plates.

The shallow layer culture method and culture system of Gexianmi are also suitable for the large-scale production of other algae of the genus Nostoc such as the fungus.

When the Gexianmi ecological simulation culture system provided by the present invention is used for large-scale cultivation of Gexianmi, it is only necessary to put the Gexianmi algae into a shallow culture tray and place it on a culture rack for cultivation.

In recent years, many researchers have invested a lot of manpower and material resources to study the artificial cultivation methods of Ge Xianmi. At present, all artificial cultivation methods related to Ge Xianmi include culture solution preparation, algae preparation, aeration culture, hardening treatment, screening and harvesting. And drying and other links, the culture container is a glass tank or a runway pool. These methods all have the problems of many production links, large water consumption, high energy consumption, easy rupture of the spheroid and high production cost. Therefore, we have designed a brand-new shallow culture system and method of Gexian rice, which can effectively solve these problems.

No matter the culture container used is a glass tank, bioreactor, water pool or raceway pool, it is necessary to mix Ge Xianmi with the culture medium. During the whole cultivation process, the Ge Xianmi must be suspended in the culture liquid through ventilation or impellers. During the nearly 90-day culture period, the culture medium needs to be replaced every 7-10 days. In the new culture system, we simplify the steps and eliminate the processes of aeration, cleaning, and changing the culture medium, which can save a lot of manpower, effectively improve work efficiency, and reduce production costs.

In the new culture system, we put Ge Xianmi in the culture tray instead of suspending cultivation of Ge Xianmi, so we can save more than 70% of water; the temperature control is not carried out by controlling the entire culture space, but It is only necessary to control the temperature of the culture medium to keep the culture at a temperature suitable for growth, thus saving more than 50% of energy consumption.

In the new culture system, it can effectively inhibit the pollution of algae, and it can reduce the pollution by more than 90% compared with the traditional method.

The rupture of Gexianmi spheres has always been a difficult problem to solve during the cultivation process. This is mainly due to the need to feed a large amount of air to suspend Gexianmi in the culture medium during the cultivation process, and the severe impact force of the air flow and the friction with the culture container make the Gexianmi spheres vulnerable to damage and severe breakage. Between 5% and 10%, the brokenness is more than 10%, and it may even completely rupture when the culture medium is replaced. And in the process of using this method to cultivate, the spheres of Ge Xianmi are rarely damaged, and the damage is less than 3%.

Compared with the prior art, the present invention has the advantages of:

1. The large-scale production system for cultivating Gexianmi provided by the present invention does not need to insulate the entire culture space, and only needs to control the temperature of the culture solution to reach the required cultivation temperature requirements of Pueraria, so the energy consumption is small ; Without relying on the air pump to suspend the algae, when the culture solution drops to the lower culture plate, because the culture solution fully contacts with the air and dissolves a large amount of air, it is enough to provide the CO ₂ and O ₂ needed for the growth of Gexianmi; due to the cultivation The hole diameter of the water outlet hole in the plate design is smaller than the diameter of Gexianmi, so when the culture solution flows out to the lower layer, various pollutants smaller than Gexianmi can flow away with the water flow, thereby reducing the pollution of algae Effect: Due to the shallow culture, the culture density is high and the culture medium required is less, so it can save more than 60% of the culture medium compared with the common culture method. Gexianmi shallow culture system requires simple technology and equipment, small investment, low production cost and continuous production throughout the year.

2, the culture method of the present invention, because what adopted is static culture method, the impact force that spheroid receives is little, and spheroid is difficult for breaking. Gexianmi has a wide range of light and temperature adaptability. It can grow at a temperature of 4-35 ℃ and a light of 600-12000lx. The photosynthetic efficiency is high. The weight gain can reach more than 6%. Gexianmi has low algae pollution rate and good stress resistance.

3. The cultivation method of the present invention can effectively reduce energy consumption and save energy by more than 50% because only the temperature of the culture solution is controlled; more than 70% of water can be saved in the cultivation process; the composition and pH of the culture solution are close to natural water bodies, It can be recycled and directly discharged without polluting the environment. This method simplifies steps and eliminates processes such as aeration, cleaning, and replacement of culture medium, effectively improving work efficiency and reducing production costs.

Description of drawings

Fig. 1 schematic diagram of the structure of the culture system of the present invention;

Fig. 2 schematic diagram of enlarged structure of the shallow culture plate of the present invention;

Among them, 1 is a multi-layer culture rack, 2 is a shallow culture plate, 3 is a lighting device, 4 is a culture liquid recovery plate, 5 is a recovery pipe, 6 is a recovery liquid filtration system, 7 is a culture liquid recovery tank, and 8 is a culture medium. Liquid delivery pump, 9 is temperature control equipment, 10 is a culture solution delivery controller, 11 is a culture solution delivery pipeline, 12 is a drip port, 13 is a vertical support rod, 14 is a horizontal support rod, 15 is a sealing plug, 16 Is a liquid level control dropper, 17 is an algae species, and 18 is a culture solution.

Detailed ways

Below in conjunction with embodiment the present invention will be further described.

Example 1

The Nostoc algae shallow culture system includes a culture solution recovery tank 7, and also includes a plurality of vertical support rods 13 for supporting and a plurality of horizontal support rods 14 for placing shallow culture trays 2. Layer culture frame 1, multiple lighting devices 3, recovered liquid filtration system 6, culture liquid delivery controller 10, culture liquid delivery pipeline 11, at least one drip port 12; Below any one of the horizontal support bars 14, the bottom of the multi-layer culture frame 1 is provided with a culture solution recovery tray 4, and the culture solution recovery tray 4 communicates with the culture solution recovery pool 7 through the recovery pipeline 5 and the recovery solution filtration system 6; The culture fluid recovery tank 7 is connected with a culture fluid delivery pump 8 and a temperature control device 9; the culture fluid delivery pump 8 communicates with the drip port 12 through the culture fluid delivery pipeline 11, and the drip port 12 is located at Above the top layer of the multi-layer culture frame 1; the bottom of the shallow layer culture tray 2 is provided with a plurality of holes, and sealing plugs 15 are arranged in the holes, and a height-adjustable liquid level control device is arranged in the middle of the sealing plugs 15. Dropper 16.

A culture solution delivery controller 10 is provided on the culture solution delivery pipeline 11 . The aperture at the bottom of the shallow culture dish 2 is 0.8-1.2 cm. The pipe diameter of described liquid level control dropper 16 is 1mm-2mm, and pipe length 4cm-8cm, and the pipe diameter of described liquid level control dropper 16 is less than the diameter of cultured algae.

The layer spacing of described multi-layer culture frame 1 is 30 cm-40cm, and layer spacing can be adjusted. The distance between the drip opening 12 and the top layer of the multi-layer culture rack 1 is 40cm-50cm.

Example 2

Taking Gexianmi as an example, the method for cultivating Gexianmi ecological simulation is cultivated with the cultivation system described in Example 1, which specifically includes:

Cultivation site and equipment: use glass greenhouse or plastic greenhouse as the training site, place the culture system in the training site, place the shallow culture tray on the multi-layer culture rack, and the interval between each layer of the multi-layer culture rack is 30 cm ～40cm, the aperture of the shallow culture plate is 1-4mm, and the distance between the drip port and the top layer of the multi-layer culture rack is set to 40cm-50cm;

Light source: The light intensity required for the growth of Gexianmi is 1000-8000lx. When the natural light intensity meets this condition, artificial light sources can be used. When the light intensity is too high, the light intensity needs to be reduced through the sunshade net. Commonly used light sources are waterproof fluorescent tubes or LED lights, and these lights are respectively arranged under the horizontal frame of the culture frame to maintain the algal body to obtain 1000-8000lx light intensity.

Temperature: The growth temperature range of Gexianmi is 10-35°C, and the optimum temperature is around 20-25°C. When the natural temperature is within this temperature range, it is only necessary to open the side membrane and the top membrane of the greenhouse for ventilation; when the temperature is too low or too high, it is necessary to maintain a suitable temperature for the culture by adjusting the temperature and flow rate of the culture medium.

The composition and concentration of the culture medium are:

MgSO ₄ . 7H ₂ O 0.05-0. 15g/L

K ₂ HPO ₄ . 3H ₂ O 0.005-0.08g/L

CaCl ₂ . 2H ₂ O 0.004-0.06g/L

EDTA-Na ₂ 0.001-0.004g/ L

Ammonium ferric citrate 0.001-0.005 g/ L

H ₃ BO ₄ 0.002-0.008mg/L

Na ₂ MoO ₄ .2H ₂ O 0.001-0.030mg/L

ZnSO ₄ .7H ₂ O 0.001-0.02mg/L

CuSO ₄ .5H ₂ O 0.001-0.005mg/L

MnCl ₂ .4H ₂ O 0.020-0.060 mg/L.

Preparation of algae seeds: There are two types of algae species: first-class and second-class. When preparing the first-grade algae species, first prepare the culture solution with distilled water, then divide it into 50-1000ml glass triangular flasks or reagent bottles, seal it, sterilize it at 121°C for 20 minutes, and cool it for later use; then select the vigorous growth and good shape. , good elasticity and pollution-free Gexianmi spheres, sterilized and homogenized in the laboratory under strict aseptic conditions, inoculated into the prepared medium, and filtered air was introduced for aeration culture; the temperature during culture was controlled at 20 -25 ℃, the light is controlled at 3000-5000lx, and an appropriate amount of red light is added. When the algal body is cultivated to a diameter of 0.5-1mm, transfer it to a 10-20L large glass bottle for expanded cultivation, and the culture conditions are the same as above; when the algae body grows to a diameter of about 2mm, check that the growth state is good and there is no pollution. secondary algae production species).

Inoculation and cultivation: Put the high-quality production seeds prepared by the above method into the shallow culture tray, control the inoculation density at 0.3 g/cm ² ~1g/cm ² , and inoculate the thickness of 0.5-2cm, and then put the shallow culture tray Place them in layers on the culture rack, add the culture medium at a certain flow rate, and control the temperature of the culture medium at 20-25°C; when the light is too strong or insufficient, it should be supplemented by sunshade or artificial light to make it meet the culture conditions. According to the diameter of the culture, select a liquid level control dropper with a diameter smaller than the diameter of Ge Xianmi particles, adjust the liquid level on the culture plate to control the height of the dropper, so that the liquid level of the culture solution poured into the culture plate just submerges the culture, The excess culture solution flows into the lower culture plate through the liquid level control dropper, and the culture solution is automatically dissolved into the fresh air when it drips into the culture plate; the dripped culture solution leaks from the upper layer to the lower layer, and finally falls into the bottom layer Liquid collection plate, then flows into the collection pipe through the outlet at the bottom of the collection plate, and enters the culture liquid collection pool after being filtered by the filter equipment, and then adjusts the temperature of the culture liquid to 20-25 °C through the temperature control equipment. The temperature in winter is slightly higher, and the temperature in summer is slightly lower. It is then reused through a drip irrigation system. The concentration of the culture solution components in the collection pool must be monitored, and the lost part must be replenished in time. During the cultivation process, when the growth of Gexianmi doubles, it is divided into two culture trays to continue cultivating, and so on, until the growth of Gexianmi reaches 6-8mm, and it is sold directly after sieving and grading or after drying in the shade.

The cultivation method has a fast growth rate of the algal species, and the average daily fresh weight gain can reach more than 6% during the cultivation process.

Product testing: It was observed by the naked eye that the Gexianmi spheres did not break, which effectively inhibited the pollution of algae, and reduced pollution by more than 70% compared with traditional methods.

Claims (6)

1. Nostoc algae shallow-layer culture systems, comprise nutrient solution pond for recovering (7), it is characterized in that, also comprising Multilayer culture shelf (1), multiple illumination apparatus (3), Ethylene recov filtering system (6), nutrient solution delivery controller (10), nutrient solution transport pipe (11), at least one liquid droping port (12) of being made up of multiple passive vertical support bar (13) and multiple horizontal support bar (14) for placing shallow-layer culture plate (2); Described illumination apparatus (3) is arranged on the below of any one horizontal support bar (14) of Multilayer culture shelf (1), described Multilayer culture shelf (1) bottom is provided with nutrient solution recovering tray (4), and described nutrient solution recovering tray (4) is communicated with nutrient solution pond for recovering (7) by recovery channel (5), Ethylene recov filtering system (6); Described nutrient solution pond for recovering (7) is provided with nutrient solution transferpump (8) and temperature control device (9) in being communicated with; Described nutrient solution transferpump (8) is communicated with liquid droping port (12) by nutrient solution transport pipe (11), and described liquid droping port (12) is positioned at the top of Multilayer culture shelf (1) top layer; Described shallow-layer culture plate (2) bottom is provided with multiple hole, is equipped with sealing plug (15) in described hole, and described sealing plug (15) centre is provided with a Height Adjustable Liquid level minim pipette (16); The caliber of described Liquid level minim pipette (16) is 1mm-2mm, pipe range 4cm-8cm, and the caliber of described Liquid level minim pipette (16) is less than the diameter of cultivated algae kind; The interlamellar spacing of described Multilayer culture shelf (1) is 30cm-40cm, and interlamellar spacing is adjustable.

2. Nostoc algae shallow-layer culture systems according to claim 1, it is characterized in that, described nutrient solution transport pipe (11) is provided with nutrient solution delivery controller (10).

3. Nostoc algae shallow-layer culture systems according to claim 1, is characterized in that, the aperture of described shallow-layer culture plate (2) bottom is 0.8-1.2cm.

4. Nostoc algae shallow-layer culture systems according to claim 1, is characterized in that, described liquid droping port (12) is 40cm-50cm with the distance of Multilayer culture shelf (1) top layer.

5. Nostoc algae shallow-layer cultural method, is characterized in that, use one of claim 1-4 described culture systems, concrete steps are:

(1) with glasshouse or plastic greenhouse greenhouse for cultivating place, described for one of claim 1-4 culture systems is placed in and cultivates in place, shallow-layer culture plate is placed on Multilayer culture shelf, the every interlayer of Multilayer culture shelf is divided into 30cm ~ 40cm, and the distance arranging liquid droping port and Multilayer culture shelf top layer is 40cm-50cm; Be provided with multiple hole bottom described shallow-layer culture plate, shallow-layer culture plate aperture is 0.8-1.2cm, is equipped with sealing plug in described hole, and described sealing plug centre is provided with a Height Adjustable Liquid level minim pipette;

(2) the inoculation algae kind of algae kind to be the diameter obtained by sterile culture be 1mm ~ 2mm, be evenly laid in shallow-layer culture plate by algae kind during inoculation, the density domination of inoculation is at 0.5g/cm ²~ 1g/cm ², inoculation thickness is 1cm-2cm;

(3) diameter is selected to be less than the Liquid level minim pipette of Nostoc particle diameter according to the diameter of culture, regulate Liquid level minim pipette height on culture plate, the nutrient solution liquid level making pouring enter culture plate just flooded culture, unnecessary nutrient solution flows into lower floor's culture plate by Liquid level minim pipette, automatically dissolves in fresh air during nutrient solution instillation culture plate; Meanwhile, controlled light remains between 1000lx ~ 20000lx, controls culture-liquid temp and remains between 20 DEG C ~ 30 DEG C;

Described nutrient solution composition and concentration are:

6. Nostoc algae shallow-layer cultural method according to claim 5, it is characterized in that, described Nostoc algae is Nostoc or Nostoc commune.