CN103897211A - Elastic cerebral dura mater - Google Patents
Elastic cerebral dura mater Download PDFInfo
- Publication number
- CN103897211A CN103897211A CN201210589564.4A CN201210589564A CN103897211A CN 103897211 A CN103897211 A CN 103897211A CN 201210589564 A CN201210589564 A CN 201210589564A CN 103897211 A CN103897211 A CN 103897211A
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- CN
- China
- Prior art keywords
- collagen
- dura mater
- cerebral dura
- dimensional structure
- type
- Prior art date
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- 230000002490 cerebral effect Effects 0.000 title abstract description 9
- 210000001951 dura mater Anatomy 0.000 title abstract description 9
- 102000012422 Collagen Type I Human genes 0.000 claims abstract description 21
- 108010022452 Collagen Type I Proteins 0.000 claims abstract description 21
- 102000008186 Collagen Human genes 0.000 claims abstract description 15
- 108010035532 Collagen Proteins 0.000 claims abstract description 15
- 229920001436 collagen Polymers 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 9
- 239000012528 membrane Substances 0.000 claims abstract description 7
- 238000004132 cross linking Methods 0.000 claims abstract description 5
- 210000001175 cerebrospinal fluid Anatomy 0.000 claims abstract description 4
- 230000000694 effects Effects 0.000 claims abstract description 4
- 210000004027 cell Anatomy 0.000 claims abstract description 3
- 230000012010 growth Effects 0.000 claims abstract description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 36
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 20
- 230000001954 sterilising effect Effects 0.000 claims description 13
- 238000004659 sterilization and disinfection Methods 0.000 claims description 11
- 230000018044 dehydration Effects 0.000 claims description 10
- 238000006297 dehydration reaction Methods 0.000 claims description 10
- 238000006116 polymerization reaction Methods 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 230000000181 anti-adherent effect Effects 0.000 claims description 2
- 230000009286 beneficial effect Effects 0.000 claims description 2
- 239000000512 collagen gel Substances 0.000 claims description 2
- 230000006835 compression Effects 0.000 claims description 2
- 238000007906 compression Methods 0.000 claims description 2
- 238000010276 construction Methods 0.000 claims description 2
- 206010015037 epilepsy Diseases 0.000 claims description 2
- 239000002362 mulch Substances 0.000 claims description 2
- 239000002985 plastic film Substances 0.000 claims description 2
- 229920006255 plastic film Polymers 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 17
- 239000012620 biological material Substances 0.000 abstract description 2
- 230000010261 cell growth Effects 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 238000004321 preservation Methods 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- 238000007493 shaping process Methods 0.000 description 8
- 238000002791 soaking Methods 0.000 description 8
- 241001269524 Dura Species 0.000 description 3
- 210000004379 membrane Anatomy 0.000 description 3
- 238000001356 surgical procedure Methods 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 210000003516 pericardium Anatomy 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 206010061598 Immunodeficiency Diseases 0.000 description 1
- 208000029462 Immunodeficiency disease Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 102000029797 Prion Human genes 0.000 description 1
- 108091000054 Prion Proteins 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000007813 immunodeficiency Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 238000007917 intracranial administration Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000000713 mesentery Anatomy 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/34—Materials or treatment for tissue regeneration for soft tissue reconstruction
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a scaffold material for the clinical reconstruction of a cerebral dura mater and a preparation method of the scaffold material, belongs to the field of the medical biomaterials, and mainly aims at solving the problems of limited drawing and complex operation of an autologous membrane, and difficulty in obtaining, high price and high risk of a xenogenous cerebral dura mater, and not easy preservation and easy sticking of biological substituent materials in clinic at present. The purpose of repairing the cerebral dura mater is achieved by providing a cerebral dura mater receptor with an active substrate for cerebral dura mater cell growth and a certain spatial three-dimensional structure and by preventing adhesion of the material to a receptor cranium by use of single-sided treatment. The material prepared by use of the method has a three-dimensional structure that a type I collagen inherently has different concentrations, and therefore, the material is good in compactness and even, has certain elasticity and tenacity, and further is pressure-resistant and capable of preventing the leakage of the cerebrospinal fluid; besides, the original activity of the collagen is maintained, and thus advantageous for the growth of the cerebral dura mater cells; in addition, the probability of post-operation adhesion of the material to the cranium is reduced by use of single-sided physical cross-linking; and the material is good in biocompatibility and thus advantageous for the repair and reconstruction of the cerebral dura mater.
Description
Technical field
The present invention relates to the biomembrane material that a kind of type i collagen makes through physics, chemical process processing.Have certain elasticity and toughness, stopping property and biocompatibility are better.For the prosthetic dural substitutes of brain surgery endocranium.Belong to biomaterial for medical purpose field.
Background technology
In world's brain surgery operation of opening cranium at present, in order to prevent that cerebrospinal fluid seepage from causing intracranial infection, the patient that need to do artificial dura mater reparation has 10%～15%.Be mainly reflected in: cerebral trauma coup injury, the infiltration of tumour, endocranium open decompression in surgical procedure, and the dura defect that causes of some geneogenous factors.The dural repairment material that used is used is autologous film, but due to the restriction of the size and dimension of being drawn materials with perform the operation numerous and diverse and produce the factors such as new wound, the autologous film of damaged employing that is mostly small area substitutes endocranium, large-area damaged or will lean on other dural substitutes to complete.
Vehicles Collected from Market dural substitutes has Homologous dura, biological substitution material endocranium.Homologous dura has two significant weakness, and the one, material source is limited and expensive; The 2nd, the most fatal weakness, the existence of prion: human body dry freeze endocranium may start an inflammation of the liver, the infection of immunodeficiency virus or bacterium etc.Biological substitution material is very effective as dural substitutes, has good biological fitness, and easy handling, wide material sources, cheap, is a kind of dural substitutes that has development potentiality.Domestic bovine pericardium, sheep pericardium, Pigs Hearts bag, ox peritonaeum, pig peritonaeum, the mesentery etc. of often using clinically replace endocranium.But there is again the adhesion of causing simultaneously, be difficult for preserving, be difficult for sterilization, the shortcomings such as immune response likely occur.
In view of this, develop a kind of good biocompatibility; There are certain elasticity and toughness; Can after generating, cambium be completely absorbed; Anti; Easily preserve, easy to operate; Material source is extensive; It is particularly important that the biological endocranium that price is relatively moderate becomes.Because natural dural main component is fiber collagen, therefore having adopted, our company utilizes own intrinsic three-dimensional structure under type i collagen different concns, by chemistry, the method that the method for physics is assembled the polymerization collagem membrane that forms metastable spatial configuration of molecules makes, the dural substitutes space structure compactness that this method makes is good, evenly, there are certain elasticity and toughness, resistance to compression, can effectively prevent cerebrospinal fluid seepage, and maintain original activity of collagen, be beneficial to the growth of endocranium cell, moreover reduce and the adhesion probability of cranium by the method for one side physical crosslinking, for endocranium reparation, the powerful guarantee that provides of epilepsy is provided.
Summary of the invention
This material selection type i collagen assembled by the method for chemistry, physics the method that forms metastable spatial configuration of molecules and made.Original activity that can maintain collagen reaches the requirement that endocranium is repaired.Main operational steps is as follows:
1, the first of type i collagen three-dimensional structure built: commercially available type i collagen, be soaked in the hydrochloric acid soln of 0.01mol/L～1.0mol/L, and make collagen solution or collagen gel that concentration is 0.1%～60.0% (w/w).
2, the processing of the fixing and anti of type i collagen three-dimensional structure: the type i collagen of the good structure of Primary Construction is carried out to plastic film mulch according to required size, then the one side of film is exposed under ultraviolet lamp, carry out UV-crosslinked, crosslinking time 30min～500min.
3, the gathering of type i collagen: by commercially available sodium hydroxide, be mixed with the solution that concentration is 0.001mol/L～2.0mol/L, sterilising treatment, is then immersed in 1min～30min in sodium hydroxide solution by the collagem membrane of and Anti-adhesive fixing through three-dimensional structure.Final pH value is (6～8).
4, the dural formation of elasticity: by the collagem membrane dehydration of having assembled, making its water content is 5.0%～40.0%, packs irradiation sterilization.
Embodiment
Embodiment 1,
Get commercially available type i collagen 1.0g, being immersed in 100ml concentration is in 0.2mol/L hydrochloric acid soln.Fixing shaping after fully soaking, crosslinked 90min in UV-crosslinked instrument.Then put in 1.0mol/L sodium hydroxide solution and soak 2min, pH value is 8.By the dehydration of gained polymerization collagen, making its water content is 40.0%.Pack rear irradiation sterilization.
Embodiment 2,
Get commercially available type i collagen 10.0g, being immersed in 100ml concentration is in 0.05mol/L hydrochloric acid soln.Fixing shaping after fully soaking, crosslinked 300min in UV-crosslinked instrument.Then put in 0.001mol/L sodium hydroxide solution and soak 15min, pH value is 7.By the dehydration of gained polymerization collagen, making its water content is 10.0%.Pack rear irradiation sterilization.
Embodiment 3,
Get commercially available type i collagen 15.0g, being immersed in 100ml concentration is in 0.5mol/L hydrochloric acid soln.Fixing shaping after fully soaking, crosslinked 90min in UV-crosslinked instrument.Then put in 2.0mol/L sodium hydroxide solution and soak 1min, pH value is 6.By the dehydration of gained polymerization collagen, making its water content is 20.0%.Pack rear irradiation sterilization.
Embodiment 4,
Get commercially available type i collagen 60.0g, being immersed in 100ml concentration is in 1.0mol/L hydrochloric acid soln.Fixing shaping after fully soaking, crosslinked 90min in UV-crosslinked instrument.Then put in 1.0mol/L sodium hydroxide solution and soak 2min, pH value is 8.By the dehydration of gained polymerization collagen, making its water content is 10.0%.Pack rear irradiation sterilization.
Embodiment 5,
Get commercially available type i collagen 0.1g, being immersed in 100ml concentration is in 0.01mol/L hydrochloric acid soln.Fixing shaping after fully soaking, crosslinked 90min in UV-crosslinked instrument.Then put in 0.001mol/L sodium hydroxide solution and soak 2min, pH value is 6.By the dehydration of gained polymerization collagen, making its water content is 30.0%.Pack rear irradiation sterilization.
Embodiment 6,
Get commercially available type i collagen 30.0g, being immersed in 100ml concentration is in 0.2mol/L hydrochloric acid soln.Fixing shaping after fully soaking, crosslinked 150min in UV-crosslinked instrument.Then put in 0.05mol/L sodium hydroxide solution and soak 2min, pH value is 6.By the dehydration of gained polymerization collagen, making its water content is 20.0%.Pack rear irradiation sterilization.
Embodiment 7,
Get commercially available type i collagen 20.0g, being immersed in 100ml concentration is in 0.1mol/L hydrochloric acid soln.Fixing shaping after fully soaking, crosslinked 20min in UV-crosslinked instrument.Then put in 1.0mol/L sodium hydroxide solution and soak 2min, pH value is 8.By the dehydration of gained polymerization collagen, making its water content is 40.0%.Pack rear irradiation sterilization.
Embodiment 8,
Get commercially available type i collagen 0.8g, being immersed in 100ml concentration is in 0.7mol/L hydrochloric acid soln.Fixing shaping after fully soaking, crosslinked 200min in UV-crosslinked instrument.Then put in 0.05mol/L sodium hydroxide solution and soak 20min, pH value is 8.By the dehydration of gained polymerization collagen, making its water content is 5.0%.Pack rear irradiation sterilization.
Claims (1)
1. assembled by the method for chemistry, physics the polymerization collagem membrane that forms metastable spatial configuration of molecules by the intrinsic three-dimensional structure of type i collagen itself for one kind, its compactness is good, evenly, there are certain elasticity and toughness, resistance to compression, can effectively prevent cerebrospinal fluid seepage, and maintain original activity of collagen, be beneficial to the growth of endocranium cell, moreover reduce and the adhesion probability of cranium by the method for one side physical crosslinking, for endocranium reparation, prevent the powerful guarantee that provides of epilepsy.Its feature comprises the following steps
(1), commercially available type i collagen, be soaked in the hydrochloric acid soln of 0.01mol/L～1.0mol/L, make collagen solution or collagen gel that concentration is 0.1%～60.0% (w/w).Just build the three-dimensional structure of certain space.
(2), the type i collagen of the good structure of Primary Construction is carried out to plastic film mulch according to required size, then the one side of film is exposed under ultraviolet lamp, carry out UV-crosslinked, crosslinking time 30～500min.The three-dimensional structure of certain space is fixed and ultraviolet processing, with anti.
(3), by commercially available sodium hydroxide, be mixed with the solution that concentration is 0.001mol/L～2.0mol/L, sterilising treatment, is then immersed in 1～30min in sodium hydroxide solution by the collagem membrane of and Anti-adhesive fixing through three-dimensional structure.Final pH value is (6～8).Assemble the three-D space structure of stablizing type i collagen.
(4), by the collagem membrane dehydration of having assembled, making its water content is 5.0%～40.0%, packs irradiation sterilization.Make elasticity endocranium.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210589564.4A CN103897211A (en) | 2012-12-26 | 2012-12-26 | Elastic cerebral dura mater |
| PCT/CN2013/000733 WO2014101254A1 (en) | 2012-12-26 | 2013-06-24 | Elastic dura mater |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210589564.4A CN103897211A (en) | 2012-12-26 | 2012-12-26 | Elastic cerebral dura mater |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103897211A true CN103897211A (en) | 2014-07-02 |
Family
ID=50988810
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210589564.4A Pending CN103897211A (en) | 2012-12-26 | 2012-12-26 | Elastic cerebral dura mater |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN103897211A (en) |
| WO (1) | WO2014101254A1 (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1463984A (en) * | 2002-06-10 | 2003-12-31 | 于海鹰 | Medicinal collagen material and its making process |
| CN103071189A (en) * | 2013-01-25 | 2013-05-01 | 广州华美康联生物科技有限公司 | Preparation method of collagen film for guided tissue regeneration |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9721585D0 (en) * | 1997-10-10 | 1997-12-10 | Geistlich Soehne Ag | Chemical product |
| JPH09122227A (en) * | 1995-10-31 | 1997-05-13 | Bio Eng Lab:Kk | Medical material and manufacturing method thereof |
| WO2006029571A1 (en) * | 2004-09-14 | 2006-03-23 | The University Of Hong Kong | Photochemically crosslinked collagen scaffolds and methods for their preparation |
| CN102716517B (en) * | 2011-03-30 | 2015-01-14 | 深圳兰度生物材料有限公司 | Guided tissue regeneration membrane and its preparation method |
-
2012
- 2012-12-26 CN CN201210589564.4A patent/CN103897211A/en active Pending
-
2013
- 2013-06-24 WO PCT/CN2013/000733 patent/WO2014101254A1/en active Application Filing
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1463984A (en) * | 2002-06-10 | 2003-12-31 | 于海鹰 | Medicinal collagen material and its making process |
| CN103071189A (en) * | 2013-01-25 | 2013-05-01 | 广州华美康联生物科技有限公司 | Preparation method of collagen film for guided tissue regeneration |
Non-Patent Citations (1)
| Title |
|---|
| JONG-EUN LEE，ET AL.: "Characterization of UV-irradiated Dense/porous Collagen Membranes：Morphology，Enzymatic Degradation，and Mechanical Properties", 《YONSEI MEDICAL JOURNAL》, vol. 42, no. 2, 31 December 2001 (2001-12-31), pages 172 - 179 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2014101254A1 (en) | 2014-07-03 |
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Application publication date: 20140702 |
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