CN103773683A - Layered-type cell culture plate - Google Patents
Layered-type cell culture plate Download PDFInfo
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- CN103773683A CN103773683A CN201210410253.7A CN201210410253A CN103773683A CN 103773683 A CN103773683 A CN 103773683A CN 201210410253 A CN201210410253 A CN 201210410253A CN 103773683 A CN103773683 A CN 103773683A
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- 238000004113 cell culture Methods 0.000 title claims abstract description 12
- 239000004809 Teflon Substances 0.000 claims abstract description 16
- 229920006362 Teflon® Polymers 0.000 claims abstract description 16
- 239000011521 glass Substances 0.000 claims abstract description 16
- 239000000758 substrate Substances 0.000 claims abstract description 11
- 210000004027 cell Anatomy 0.000 claims description 25
- 239000003351 stiffener Substances 0.000 claims description 22
- 238000001914 filtration Methods 0.000 claims description 19
- 230000010261 cell growth Effects 0.000 claims description 12
- 210000002421 cell wall Anatomy 0.000 claims description 10
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 239000012930 cell culture fluid Substances 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 6
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 230000009286 beneficial effect Effects 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 230000002411 adverse Effects 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 230000003822 cell turnover Effects 0.000 claims description 3
- 210000004748 cultured cell Anatomy 0.000 claims description 3
- 230000000249 desinfective effect Effects 0.000 claims description 3
- 239000007789 gas Substances 0.000 claims description 3
- 230000009545 invasion Effects 0.000 claims description 3
- 238000011068 loading method Methods 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 230000029058 respiratory gaseous exchange Effects 0.000 claims description 3
- 241001515965 unidentified phage Species 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 238000013461 design Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000001464 adherent effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002380 cytological effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
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Abstract
分层式细胞培养板，属于医疗器械技术领域，分层式细胞培养板由培养板、培养板孔、培养板槽、培养板槽壁、微孔滤膜、板孔立柱、培育板轴孔、培育孔、培育板、培养板孔壁、培养板孔腔、支撑柱、培育孔壁、特氟龙层、培育孔开口、培育孔底、培育孔基板组成，其特征在于培养板由玻璃制成，上部向内有一个培养板槽，在培养板槽中，有6个培养板孔；培养板孔上半部分高出培养板槽底部，培养板孔中央有一根板孔立柱，在板孔立柱穿过培育板轴孔和支撑柱后，即可将培育板一层层地叠加在一起；支撑柱是将培育板相互隔开2毫米的玻璃圆筒；在培育板的上表面规则排列着多个培育孔。分层式细胞培养板制作简单，可操作性强，成本低廉，效果明显。
A layered cell culture plate belongs to the technical field of medical devices. The layered cell culture plate consists of a culture plate, a culture plate hole, a culture plate groove, a culture plate groove wall, a microporous filter membrane, a plate hole column, a culture plate shaft hole, The cultivation hole, the cultivation plate, the cultivation plate hole wall, the cultivation plate cavity, the support column, the cultivation well wall, the Teflon layer, the cultivation well opening, the cultivation well bottom, and the cultivation well substrate are characterized in that the cultivation plate is made of glass , there is a culture plate groove in the upper part, and there are 6 culture plate holes in the culture plate groove; After passing through the shaft hole of the cultivation plate and the support column, the cultivation plate can be stacked together layer by layer; the support column is a glass cylinder that separates the cultivation plate from each other by 2 mm; cultivation hole. The layered cell culture plate is easy to manufacture, has strong operability, low cost and obvious effect.
Description
Technical field
The present invention relates to a kind of layer-stepping Tissue Culture Plate, belong to medical treatment and laboratory equipment technical field.
Background technology
At present, in cytological research process, often need to cultivate cell, to meet the specific requirement of various experiments, but, in cell cultivation process, because need sterilization, conventional method is to carry out the experiment of cytobiology with various disposable consumptive materials, the price of every kind of consumptive material is all higher, such as six orifice plates just need 7 yuans, once tests, just need polylith six orifice plates, increase exponentially thereby make to test needed expense.In cell cultivation process, cell whether cultivate one of successful mark be cell whether adherent, adherent after whether fissiparity.Like this, just need the larger needed Area of bearing of Growth of Cells in order to obtain result preferably.How to reduce the cost of cell cultures, on a Tissue Culture Plate, turn out more cell and become a great problem of being badly in need of solution? so utilize the design of layering and the cultivation hole that increases cell attachment area is set on every one deck, inventing a kind of is necessary at the layer-stepping Tissue Culture Plate of cultivating plate hole and have multilayer breezing plate.
Summary of the invention
In order to overcome the less difficult problem of the higher culture area of known Tissue Culture Plate cost, the invention provides a kind of layer-stepping Tissue Culture Plate, this layer-stepping Tissue Culture Plate utilizes breezing plate that cultivation plate hole is divided into multilayer and cultivates face, on each cultivation face, design again multiple cultivations hole, the growth needs supporting plane of cell, because layering adds the design in multiple cultivations hole, expand the area of Growth of Cells, the more known culture plate of culture area of layer-stepping Tissue Culture Plate is significantly improved, the use of glass and Teflon, can also make culture plate be reused, can reduce the rear waste abandoning resource for known batch cultur plate, thereby reach the object that reduces cell cultures expense.
The technical solution adopted for the present invention to solve the technical problems is:
Layer-stepping Tissue Culture Plate by culture plate 1, cultivate plate hole 2, cultivate board slot 3, culture plate cell wall 4, millipore filtration 5, plate hole column 6, breezing plate axis hole 7, cultivate hole 8, breezing plate 9, culture plate hole wall 10, culture plate vestibule 11, pillar stiffener 12, cultivate hole wall 13, Teflon layer 14, cultivate hole opening 15, cultivate at the bottom of hole 16, cultivate hole substrate 17 and form, it is characterized in that culture plate 1 is made up of glass, the cultivation board slot 3 that a degree of depth is 5-10 millimeter is inwardly arranged at top, cultivating in board slot 3, there are 6 to cultivate plate hole 2; Cultivate plate hole 2 upper part and exceed cultivation board slot 3 bottoms, seal so that millipore filtration 5 can wrap after the opening outside of cultivation plate hole 2 is wound around the opening of cultivating plate hole 2; Cultivating plate hole 2 central authorities has a plate hole column 6, after plate hole column 6 is through breezing plate axis hole 7 and pillar stiffener 12, breezing plate 9 can be superimposed from level to level; Pillar stiffener 12 is breezing plate 9 to be spaced from each other to the glass cylinder of 2 millimeters; At the upper surface of breezing plate 9, breezing plate axis hole 7 peripheries are that 2 millimeters, the degree of depth are the cultivation hole 8 of 2 millimeters to regularly arranged multiple diameters between breezing plate 9 peripheries, and cultivating hole 8 is main place of Growth of Cells.
Preferred version of the present invention is: in cultivation board slot 3, having 6 diameters is 3-3.5 centimetre, and the degree of depth is the cultivation plate hole 2 of 1.0-1.8 centimetre; Cultivate plate hole 2 upper part and exceed cultivation board slot 3 bottom 4-9 millimeters, so that millipore filtration 5 can wrap the opening of cultivating plate hole 2, allow the pollution of cultivating cell in plate hole 2 and both can avoid bacterium, can freely obtain from the outside again oxygen, be beneficial to the breathing of cell, prevent that anoxic from bringing adverse influence to cultured cells.
Preferred version of the present invention is: cultivating board slot 3 outwardly convex is around culture plate cell wall 4, culture plate cell wall 4 upper surfaces with cultivate the opening of plate hole 2 and superscribe surface after millipore filtration 5 in same level, be convenient to packed and transported, when culturing cell, can also guarantee the performance attractive in appearance of culture plate.
Preferred version of the present invention is: millipore filtration 5 is that commercially available can guarantee moisture and gas passes through, but can stop the invasion and attack of bacteriophage, on the Bechtop disinfecting, after with millipore filtration 5, the opening of cultivating plate hole 2 being wound around, seal, be then placed under optimal temperature and cultivate.
Preferred version of the present invention is: cultivating plate hole 2 central authorities, to have a diameter be vitreous plate hole column 6 of 2 millimeters, after plate hole column 6 is through breezing plate axis hole 7 and pillar stiffener 12, breezing plate 9 can be superimposed from level to level, fill the culture plate vestibule 11 being surrounded by culture plate hole wall 10.
Preferred version of the present invention is: it is that 0.5 centimetre, length are the glass cylinder of 2 millimeters that adjacent two breezing plates 9 are spaced from each other 2 millimeters, diameter by pillar stiffener 12, and barrel bore is 2.2 millimeters, and cylindrical wall thickness is 1.4 millimeters; Pillar stiffener 12 is positioned at breezing plate 9 central authorities belows, and connects as one with breezing plate 9, and the cylinder bore of pillar stiffener 12 is connected up and down with the vestibule of breezing plate axis hole 7, and internal diameter size is 2.2 millimeters.
Preferred version of the present invention is: breezing plate 9 is that vitreous diameter is that 2.5-3 centimetre, thickness are the plectane of 2.5-3 millimeter, plectane edge and the distance of cultivating between plate hole 2 walls are 2 millimeters, so that band needs the nutrient solution of culturing cell and injects and cultivate in plate hole 2, the diameter of plectane central authorities is that the hole of 2.2 millimeters is breezing plate axis hole 7.
Preferred version of the present invention is: cultivating hole 8 and be at the upper surface of breezing plate 9, breezing plate axis hole 7 peripheries is that 2 millimeters, the degree of depth are the hole of 2 millimeters to regularly arranged between breezing plate 9 peripheries, multiple diameters, and cultivation hole 8 is main place of Growth of Cells; Each cultivation hole 8 includes and cultivates hole wall 13, Teflon layer 14, cultivate hole opening 15, cultivate at the bottom of hole 16, cultivate hole substrate 17; At the bottom of cultivating hole wall 13 and cultivating hole, 16 surround the inner chamber of cultivating hole 8 jointly, glass quality, 16 surface at the bottom of Teflon can being added to breezing plate 9 upper surfaces and cultivation hole wall 13 and cultivating hole according to known loading method, forming thickness is the Teflon layer 14 of 0.1-0.2 millimeter, be conducive to Growth of Cells and by use after to whole culture plate 1 cleaning and sterilizing, in order to next time use; The opening of cultivating 8 tops, hole is cultivates hole opening 15, and diameter is 2 millimeters, is that the passage in hole 8 is cultivated in the cell turnover of cultivating hole 8 interior cultivations; Cultivate hole substrate 17 and be breezing plate 9 lower surfaces to cultivate at the bottom of hole between 16 part, each can be cultivated to hole 8 and connect into breezing plate 9.
Preferred version of the present invention is: layer-stepping Tissue Culture Plate operating process is: while carrying out cell cultures, first by whole culture plate 1 cleaning and sterilizing, then in the gnotobasis of Bechtop, cell culture fluid is filled into and is cultivated in plate hole 2, be provided with multilayer breezing plate 9 owing to cultivating in plate hole 2, fill and cultivate after plate hole 2, cell and nutrient solution thereof can arrive cultivates each corner in plate hole 2, certainly also comprise each cultivation hole 8, then, the cell culture fluid of cultivating plate hole 2 preglabellar fields is siphoned away with suction pipe, cell can grow cultivating in hole 8.
Beneficial effect of the present invention is, layer-stepping Tissue Culture Plate utilizes breezing plate that cultivation plate hole is divided into multilayer and cultivates face, on each cultivation face, design again multiple cultivations hole, the growth needs supporting plane of cell, because layering adds the design in multiple cultivations hole, expand the area of Growth of Cells, the more known culture plate of culture area of layer-stepping Tissue Culture Plate is significantly improved, the use of glass and Teflon, can also make culture plate be reused, can reduce the rear waste abandoning resource for known batch cultur plate, thereby reach the object that reduces cell cultures expense.Layer-stepping Tissue Culture Plate is made simple, workable, with low cost, successful.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the present invention is further described.
Fig. 1 is the one-piece construction schematic diagram of layer-stepping Tissue Culture Plate of the present invention.
Fig. 2 is the cultivation plate hole vertical section structure schematic diagram of layer-stepping Tissue Culture Plate of the present invention.
Fig. 3 is the breezing plate structural representation of layer-stepping Tissue Culture Plate of the present invention.
Fig. 4 is the support column arrangement schematic diagram of layer-stepping Tissue Culture Plate of the present invention.
Fig. 5 is the cultivation pore structure schematic diagram of layer-stepping Tissue Culture Plate of the present invention.
1. culture plates in figure, 2. cultivate plate hole, 3. cultivate board slot, 4. culture plate cell wall, 5. millipore filtration, 6. plate hole column, 7. breezing plate axis hole, 8. cultivates hole, 9. breezing plate, 10. culture plate hole wall, 11. culture plate vestibules, 12. pillar stiffeners, 13. cultivate hole wall, 14. Teflon layers, and 15. cultivate hole opening, at the bottom of 16. cultivation holes, 17. cultivate hole substrates.
Embodiment
Embodiment mono-:
Layer-stepping Tissue Culture Plate by culture plate 1, cultivate plate hole 2, cultivate board slot 3, culture plate cell wall 4, millipore filtration 5, plate hole column 6, breezing plate axis hole 7, cultivate hole 8, breezing plate 9, culture plate hole wall 10, culture plate vestibule 11, pillar stiffener 12, cultivate hole wall 13, Teflon layer 14, cultivate hole opening 15, cultivate at the bottom of hole 16, cultivate hole substrate 17 and form, it is characterized in that culture plate 1 is made up of glass, the cultivation board slot 3 that a degree of depth is 5-10 millimeter is inwardly arranged at top, cultivating in board slot 3, there are 6 to cultivate plate hole 2; Cultivate plate hole 2 upper part and exceed cultivation board slot 3 bottoms, seal so that millipore filtration 5 can wrap after the opening outside of cultivation plate hole 2 is wound around the opening of cultivating plate hole 2; Cultivating plate hole 2 central authorities has a plate hole column 6, after plate hole column 6 is through breezing plate axis hole 7 and pillar stiffener 12, breezing plate 9 can be superimposed from level to level; Pillar stiffener 12 is breezing plate 9 to be spaced from each other to the glass cylinder of 2 millimeters; At the upper surface of breezing plate 9, breezing plate axis hole 7 peripheries are that 2 millimeters, the degree of depth are the cultivation hole 8 of 2 millimeters to regularly arranged multiple diameters between breezing plate 9 peripheries, and cultivating hole 8 is main place of Growth of Cells.
In cultivation board slot 3, having 6 diameters is 3-3.5 centimetre, and the degree of depth is the cultivation plate hole 2 of 1.0-1.8 centimetre; Cultivate plate hole 2 upper part and exceed cultivation board slot 3 bottom 4-9 millimeters, so that millipore filtration 5 can wrap the opening of cultivating plate hole 2, allow the pollution of cultivating cell in plate hole 2 and both can avoid bacterium, can freely obtain from the outside again oxygen, be beneficial to the breathing of cell, prevent that anoxic from bringing adverse influence to cultured cells.
Cultivating board slot 3 outwardly convex is around culture plate cell wall 4, culture plate cell wall 4 upper surfaces with cultivate the opening of plate hole 2 and superscribe surface after millipore filtration 5 in same level, be convenient to packed and transported, when culturing cell, can also guarantee the performance attractive in appearance of culture plate.
Millipore filtration 5 is that commercially available can guarantee moisture and gas passes through, but can stop the invasion and attack of bacteriophage, on the Bechtop disinfecting, after with millipore filtration 5, the opening of cultivating plate hole 2 being wound around, seals, and is then placed under optimal temperature and cultivates.
Cultivating plate hole 2 central authorities, to have a diameter be vitreous plate hole column 6 of 2 millimeters, after plate hole column 6 is through breezing plate axis hole 7 and pillar stiffener 12, breezing plate 9 can be superimposed from level to level, fill the culture plate vestibule 11 being surrounded by culture plate hole wall 10.
It is that 0.5 centimetre, length are the glass cylinder of 2 millimeters that adjacent two breezing plates 9 are spaced from each other 2 millimeters, diameter by pillar stiffener 12, and barrel bore is 2.2 millimeters, and cylindrical wall thickness is 1.4 millimeters; Pillar stiffener 12 is positioned at breezing plate 9 central authorities belows, and connects as one with breezing plate 9, and the cylinder bore of pillar stiffener 12 is connected up and down with the vestibule of breezing plate axis hole 7, and internal diameter size is 2.2 millimeters.
Cultivating hole 8 and be at the upper surface of breezing plate 9, breezing plate axis hole 7 peripheries is that 2 millimeters, the degree of depth are the hole of 2 millimeters to regularly arranged between breezing plate 9 peripheries, multiple diameters, and cultivation hole 8 is main place of Growth of Cells; Each cultivation hole 8 includes and cultivates hole wall 13, Teflon layer 14, cultivate hole opening 15, cultivate at the bottom of hole 16, cultivate hole substrate 17; At the bottom of cultivating hole wall 13 and cultivating hole, 16 surround the inner chamber of cultivating hole 8 jointly, glass quality, 16 surface at the bottom of Teflon can being added to breezing plate 9 upper surfaces and cultivation hole wall 13 and cultivating hole according to known loading method, forming thickness is the Teflon layer 14 of 0.1-0.2 millimeter, be conducive to Growth of Cells and by use after to whole culture plate 1 cleaning and sterilizing, in order to next time use; The opening of cultivating 8 tops, hole is cultivates hole opening 15, and diameter is 2 millimeters, is that the passage in hole 8 is cultivated in the cell turnover of cultivating hole 8 interior cultivations; Cultivate hole substrate 17 and be breezing plate 9 lower surfaces to cultivate at the bottom of hole between 16 part, each can be cultivated to hole 8 and connect into breezing plate 9.
The operating process of layer-stepping Tissue Culture Plate is: while carrying out cell cultures, first by whole culture plate 1 cleaning and sterilizing, then in the gnotobasis of Bechtop, cell culture fluid is filled into and is cultivated in plate hole 2, be provided with multilayer breezing plate 9 owing to cultivating in plate hole 2, fill and cultivate after plate hole 2, cell and nutrient solution thereof can arrive cultivates each corner in plate hole 2, certainly also comprise each cultivation hole 8, then, the cell culture fluid of cultivating plate hole 2 preglabellar fields is siphoned away with suction pipe, cell can grow cultivating in hole 8.
More than show and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should be appreciated that; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification sheets, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications; these changes and improvements all fall in the claimed scope of the invention, and the claimed scope of the present invention is defined by appending claims equivalent.
Claims (9)
1. layer-stepping Tissue Culture Plate is by culture plate (1), cultivate plate hole (2), cultivate board slot (3), culture plate cell wall (4), millipore filtration (5), plate hole column (6), breezing plate axis hole (7), cultivate hole (8), breezing plate (9), culture plate hole wall (10), culture plate vestibule (11), pillar stiffener (12), cultivate hole wall (13), Teflon layer (14), cultivate hole opening (15), at the bottom of cultivation hole (16), cultivate hole substrate (17) composition, it is characterized in that culture plate (1) is made up of glass, the cultivation board slot (3) that a degree of depth is 5-10 millimeter is inwardly arranged at top, in cultivation board slot (3), there are 6 to cultivate plate hole (2), cultivate plate hole (2) upper part and exceed cultivation board slot (3) bottom, seal so that millipore filtration (5) can wrap after the opening outside of cultivation plate hole (2) is wound around the opening of cultivating plate hole (2), cultivate plate hole (2) central authorities and have a plate hole column (6), after plate hole column (6) is through breezing plate axis hole (7) and pillar stiffener (12), breezing plate (9) can be superimposed from level to level, pillar stiffener (12) is that breezing plate (9) is spaced from each other to the glass cylinder of 2 millimeters, at the upper surface of breezing plate (9), breezing plate axis hole (7) periphery is that 2 millimeters, the degree of depth are the cultivation hole (8) of 2 millimeters to regularly arranged multiple diameters between breezing plate (9) periphery, and cultivating hole (8) is the main place of Growth of Cells.
2. layer-stepping Tissue Culture Plate according to claim 1, is characterized in that having 6 diameters is 3-3.5 centimetre in cultivation board slot (3), and the degree of depth is the cultivation plate hole (2) of 1.0-1.8 centimetre; Cultivate plate hole (2) upper part and exceed cultivation board slot (3) bottom 4-9 millimeter, so that millipore filtration (5) can wrap the opening of cultivating plate hole (2), allow the interior cell of cultivation plate hole (2) both can avoid the pollution of bacterium, can freely obtain from the outside again oxygen, be beneficial to the breathing of cell, prevent that anoxic from bringing adverse influence to cultured cells.
3. layer-stepping Tissue Culture Plate according to claim 1, it is characterized in that cultivating board slot (3) outwardly convex is around culture plate cell wall (4), culture plate cell wall (4) upper surface superscribes surface after millipore filtration (5) in same level with the opening of cultivating plate hole (2), be convenient to packed and transported, when culturing cell, can also guarantee the performance attractive in appearance of culture plate.
4. layer-stepping Tissue Culture Plate according to claim 1, it is characterized in that millipore filtration (5) is that commercially available can guarantee moisture and gas passes through, but can stop the invasion and attack of bacteriophage, on the Bechtop disinfecting, after the opening of cultivating plate hole (2) being wound around with millipore filtration (5), seal, be then placed under optimal temperature and cultivate.
5. layer-stepping Tissue Culture Plate according to claim 1, it is characterized in that to have a diameter be vitreous plate hole column (6) of 2 millimeters cultivating plate hole (2) central authorities, after plate hole column (6) is through breezing plate axis hole (7) and pillar stiffener (12), breezing plate (9) can be superimposed from level to level, fill the culture plate vestibule (11) being surrounded by culture plate hole wall (10).
6. layer-stepping Tissue Culture Plate according to claim 1, it is characterized in that it is that 0.5 centimetre, length are the glass cylinder of 2 millimeters that adjacent two breezing plates (9) are spaced from each other 2 millimeters, diameter by pillar stiffener (12), barrel bore is 2.2 millimeters, and cylindrical wall thickness is 1.4 millimeters; Pillar stiffener (12) is positioned at breezing plate (9) central authorities below, and connect as one with breezing plate (9), the cylinder bore of pillar stiffener (12) is connected up and down with the vestibule of breezing plate axis hole (7), and internal diameter size is 2.2 millimeters.
7. layer-stepping Tissue Culture Plate according to claim 1, it is characterized in that breezing plate (9) is that vitreous diameter is that 2.5-3 centimetre, thickness are the plectane of 2.5-3 millimeter, plectane edge and the distance of cultivating between plate hole (2) wall are 2 millimeters, so that band needs the nutrient solution of culturing cell and injects and cultivate in plate hole (2), the diameter of plectane central authorities is that the hole of 2.2 millimeters is breezing plate axis hole (7).
8. layer-stepping Tissue Culture Plate according to claim 1, it is characterized in that cultivating hole (8) and be at the upper surface of breezing plate (9), breezing plate axis hole (7) periphery is that 2 millimeters, the degree of depth are the hole of 2 millimeters to regularly arranged between breezing plate (9) periphery, multiple diameters, and cultivating hole (8) is the main place of Growth of Cells; Each cultivation hole (8) includes and cultivates hole wall (13), Teflon layer (14), cultivate hole opening (15), cultivate (16) at the bottom of hole, cultivate hole substrate (17); At the bottom of cultivating hole wall (13) and cultivating hole, (16) surround the inner chamber of cultivating hole (8) jointly, glass quality, the surface of (16) at the bottom of Teflon can being added to breezing plate (9) upper surface and cultivation hole wall (13) and cultivating hole according to known loading method, forming thickness is the Teflon layer (14) of 0.1-0.2 millimeter, be conducive to Growth of Cells and by use after to whole culture plate (1) cleaning and sterilizing, in order to next time use; The opening of cultivating top, hole (8) is cultivates hole opening (15), and diameter is 2 millimeters, is to cultivate the cell turnover of cultivating in hole (8) to cultivate the passage in hole (8); To cultivate hole substrate (17) be breezing plate (9) lower surface between (16) at the bottom of cultivating hole part, each can be cultivated to hole (8) and connect into breezing plate (9).
9. layer-stepping Tissue Culture Plate according to claim 1, it is characterized in that operating process is: while carrying out cell cultures, first by whole culture plate (1) cleaning and sterilizing, then in the gnotobasis of Bechtop, cell culture fluid is filled into and is cultivated in plate hole (2), be provided with multilayer breezing plate (9) owing to cultivating in plate hole (2), fill and cultivate after plate hole (2), cell and nutrient solution thereof can arrive cultivates each corner in plate hole (2), certainly also comprise each cultivation hole (8), then, the cell culture fluid of cultivating plate hole (2) preglabellar field is siphoned away with suction pipe, cell can grow in cultivation hole (8).
Priority Applications (1)
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| CN201210410253.7A CN103773683B (en) | 2012-10-17 | 2012-10-17 | Layer-stepping Tissue Culture Plate |
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| CN201210410253.7A CN103773683B (en) | 2012-10-17 | 2012-10-17 | Layer-stepping Tissue Culture Plate |
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| CN103773683B CN103773683B (en) | 2015-11-25 |
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Cited By (5)
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| CN105505745A (en) * | 2016-01-15 | 2016-04-20 | 北京酷搏科技有限公司 | Perforated plate and perforated plate assembly including same |
| CN109097274A (en) * | 2017-06-20 | 2018-12-28 | 中国科学院大连化学物理研究所 | The standardized high-throughput three-dimensional cell preparation facilities of one kind and its application |
| CN109187937A (en) * | 2018-08-20 | 2019-01-11 | 河南科技大学第附属医院 | Capillary type cancer cell culture detects grower |
| CN114733472A (en) * | 2022-04-24 | 2022-07-12 | 中国科学院长春应用化学研究所 | Reaction kettle suitable for multistage reaction |
| WO2025108502A1 (en) * | 2023-11-23 | 2025-05-30 | 苏州喜同生物科技有限公司 | Moat-type microwell culture plate |
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| WO2025108502A1 (en) * | 2023-11-23 | 2025-05-30 | 苏州喜同生物科技有限公司 | Moat-type microwell culture plate |
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| CN103773683B (en) | 2015-11-25 |
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