CN103750016B - A kind of liquid feed for raising Phytoseiidae Predatory Mites and preparation method thereof - Google Patents

Abstract

The invention discloses a liquid feed for raising phytoseiidae predatory mites and a preparation method thereof. The liquid feed includes vitamin B complex? 5~20mg/mL, vitamin C? 5-20mg/mL and the following components in volume percentage: 10%-30% of egg yolk, 10%-30% of milk, 2%-10% of honey, 10%-50% of fly maggot homogenate, and even Serum 10%-50%, preservative 0.5%-3%, and the balance is water. The compound vitamin B and vitamin C are grinded according to the dose respectively, then the above-mentioned other substances are added and mixed, the remaining amount is made up with water, and the liquid feed is obtained by stirring evenly. The liquid feed of the invention is simple to make and low in cost, can fully meet the nutritional requirements of the phytoseiidae predator mites, has good breeding effect, and can be used for large-scale industrial production of the predator mites.

Description

A liquid feed for raising phytoseiidae predatory mites and its preparation method

technical field

The invention belongs to the technical field of raising predatory mites. More specifically, it relates to a liquid feed for feeding phytoseiidae predatory mites and a preparation method thereof.

Background technique

The widespread occurrence and rampant damage of agricultural and forestry crop pest mites (including spider mites, rust ticks, tarsal mites, and acaroid mites) has become a worldwide problem. Small insects have become a catastrophe, which has been the development trend of pests in agricultural and forestry production in my country in recent years. For example, the red spider mites on cotton have risen to become the main pests. , Rust ticks harm citrus, seriously affecting the yield and quality of citrus. Acaroid mites, cats and tarsus mites harm edible fungi such as mushrooms, white fungus, and shiitake mushrooms, causing devastating losses such as rotten beds and seeds; in addition, mites harm urban gardens, flowers, tea gardens, and greenhouse vegetables to varying degrees.

At present, my country's crop pest mites are still dominated by chemical control. Extensive use of pesticides has led to a sharp increase in the resistance of pest mites, forcing the use of pesticides to increase the dosage or use highly toxic pesticides, resulting in serious residues in crops, polluting the environment, and seriously endangering human health. The use of biological control strategies, especially the technology of "controlling mites with mites" has become the best way to control the harm of mites in the world today.

The breeding of predatory mites such as Cucumber Amblyseius mainly relies on the leftovers of agricultural products to cultivate acaroid mites, and uses acaroid mites as food to cultivate predatory mites. promotional application.

Contents of the invention

The technical problem to be solved by the present invention is to overcome the technical deficiencies in the existing predatory mite breeding, and provide a liquid feed that has low requirements on the feeding environment and can be used for large-scale industrial production of predatory mites, especially for feeding Phytoseiidae predatory Liquid feed for mites.

Another object of the present invention is to provide a method for preparing the liquid feed for feeding predatory mites of Phytoseiidae.

The above object of the present invention is achieved through the following technical solutions:

The invention provides a liquid feed for raising predatory mites of Phytoseiidae, the liquid feed includes the components of vitamin B5-20mg/mL, vitamin C5-20mg/mL and the following components in volume percentage : Egg yolk 10% ~ 30%, milk 10% ~ 30%, honey 2% ~ 10%, fly maggot homogenate 10% ~ 50%, acarina homogenate 10% ~ 50% and preservatives, 0.5% ~ 3 %, the balance is water.

Preferably, the liquid feed includes the components of complex vitamin B5-15mg/mL, vitamin C5-15mg/mL and the following components in volume percentage: 10%-20% of egg yolk, 10%-20% of milk, 2% to 5% honey, 10% to 20% fly maggot homogenate, 10% to 20% acaroid homogenate and 0.5% to 2% preservative, and the balance is water.

More preferably, the liquid feed includes the components of complex vitamin B5-10mg/mL, vitamin C5-10mg/mL and the following components in volume percentage: 10%-15% of egg yolk, 10%-15% of milk , honey 4% to 5%, fly maggot homogenate 10% to 15%, acaroid mite homogenate 10% to 15%, preservative 0.5% to 1%, and the balance is water.

Preferably, the milk is commercially available pure milk products, commercially available fresh milk products or fresh milk just produced by sterilized cows.

The fly maggot homogenate is that the fly maggot larvae are added into water to homogenate, the homogenate is centrifuged, and the supernatant is taken to obtain the fly maggot homogenate; Homogenize the homogenate, centrifuge the homogenate, and take the supernatant to obtain the acarina homogenate.

Preferably, the Phytoseiidae predatory mite is Neoseiuluscucumeris , Amblyseiusbarkeri , Amblyseiusswirskii or Neoseiulusswirskii .

More preferably, the Phytoseiidae predatory mite is A. cucurbita or A. pastereii.

Preferably, the preservative is one or a combination of potassium sorbate, sodium benzoate, lactis streptococcus, natamycin, sodium dehydroacetate, propyl p-hydroxybenzoate or dehydroacetic acid; When used in combination, the mixing ratio is not strictly limited.

More preferably, the preservative is one or a combination of potassium sorbate, sodium benzoate, or propyl p-hydroxybenzoate; when used in combination, the mixing ratio is not strictly limited.

The present invention also provides a method for preparing the liquid feed for raising phytoseiidae predatory mites, the steps are as follows:

S1. Add the fly maggot larvae to water, homogenate, centrifuge the homogenate, and take the supernatant for subsequent use;

Take acaroid mites and add water to homogenate, centrifuge the homogenate, and take the supernatant for later use;

Weigh egg yolk, milk and honey in proportion, and set aside;

The complex vitamin B and vitamin C are ground into powder respectively and set aside;

S2. Mix the components prepared in S1 evenly, add preservatives, make up the remaining amount with water, and stir well to obtain liquid feed.

As a preference, the components of the liquid feed in the present invention and the water used to prepare the liquid feed are sterile water; aseptic conditions are more conducive to long-term preservation of the liquid feed.

The present invention has the following beneficial effects:

The present invention solves the technical problems and deficiencies in the artificial breeding of predatory mites such as A. cucurbita A. cucurbita, such as many processes, takes up a large space, and consumes labor, and provides a liquid feed for raising predatory mites of Phytoseiaceae, and the Feed raw materials are easy to find, low in cost, simple to make, and more nutritious and reasonable.

The liquid feed of the present invention is used to raise predatory mites, can lay eggs normally, hatch, and mature, and the feeding effect is good; and the space occupied is small, and the feeding environment is cleaner, and it is suitable for A. cucurbita or A. pastereii, etc. Large-scale industrial production of predatory mites.

Detailed ways

The present invention will be further described below in conjunction with specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the technical field.

Example 1

1. Preparation of liquid feed

The preparation method of liquid feed is as follows:

S1. Add 15ml sterile water to 20 fly maggot larvae and homogenate in an electric homogenizer, then centrifuge the homogenate, and get 10ml supernatant for subsequent use;

S2. Get 5g of acarina and add 15ml of sterile water to homogenize in an electric homogenizer, then centrifuge the homogenate, and get 10ml of supernatant for subsequent use;

S3. Take 15ml of egg yolk for later use;

S4. Take 15ml of milk for later use;

S5. Take 5ml of honey for later use;

S6. After grinding the complex vitamin B and vitamin C into powder respectively, take 1g each according to the dose and place it in the container;

S7. Mix the components prepared in S1-S6 into the container of S6, add 1ml of potassium sorbate, make up the remaining amount to 100ml with sterile water, and stir well.

2. The liquid feed is made into small capsules with an elongated wrapping material (the said wrapping material is a stretchable sealing film or a stretchable plastic wrap, the same as in the following examples) (the size of the small capsule is roughly 1 ~2cm, but this does not limit the present invention), placed in a culture device, inserted Amblyseius barkeri ( Amblyseiusbarkeri ) for culture and breeding. The conditions for culturing and breeding are temperature 25°C and relative humidity 70%.

The results showed that after about 10 days of feeding, Amblyseius pastereii could multiply rapidly, and the amount of Amblyseius pastereii increased about 2 to 10 times per week.

Example 2

1. Preparation of liquid feed

The preparation method of liquid feed is as follows:

S1. Add 15ml sterile water to 20 fly maggot larvae and homogenate in an electric homogenizer, then centrifuge the homogenate, and get 13ml supernatant for subsequent use;

S2. Get 5g of acarina and add 10ml of sterile water to homogenate in an electric homogenizer, then centrifuge the homogenate, and get 13ml of supernatant for subsequent use;

S3. Take 12ml of egg yolk for later use;

S4. Take 12ml of milk for later use;

S5. Take 5ml of honey for later use;

S6. after the compound vitamin B and vitamin C are ground into powder respectively, take 0.8g according to the dose and place them in the container;

S7. Mix the components prepared in S1-S6 into the container of S6, add 0.8ml of sodium benzoate, make up the rest to 100ml with sterile water, and stir well.

2. Make the liquid feed into small capsules with elongated wrapping material (the wrapping material is stretchable sealing film or stretchable plastic wrap) (the size of the small capsule is roughly 1-2cm, but not Therefore, the present invention is defined), placed in a culture device, and inserted into Neoseiulus cucumeris for culture and breeding. The conditions for cultivation and breeding are temperature 26°C and relative humidity 80%.

The results showed that after about 10 days of feeding, A. cucurbita could rapidly multiply, and the amount of A. cucurbita increased by about 2 to 10 times per week.

Example 3

1. Preparation of liquid feed

The preparation method of liquid feed is as follows:

S1. Add 15ml sterile water to 20 fly maggot larvae and homogenate in an electric homogenizer, then centrifuge the homogenate, and get 11ml supernatant for subsequent use;

S2. Get 5g of acarid mites and add 10ml of sterile water to homogenate in an electric homogenizer, then centrifuge the homogenate, and get 11ml of supernatant for subsequent use;

S3. Take 14ml egg yolk for later use;

S4. Take 14ml of milk for later use;

S5. Take 4ml of honey for later use;

S6. After grinding the complex vitamin B and vitamin C into powder respectively, take 0.5g each according to the dose and place it in the container;

S7. Mix the components prepared in S1-S6 into the container of S6, add 1ml of propylparaben, make up the rest to 100ml with sterile water, and stir well.

2. Make the liquid feed into small capsules with elongated wrapping materials (the above-mentioned wrapping materials are stretchable sealing film or stretchable plastic wrap) (the size of the small capsules is roughly 1-2cm, but not Therefore limit the present invention), place it in a culture device, insert Amblyseius pasteurii for culture and breeding. The conditions for culturing and breeding are temperature at 27°C and relative humidity at 70%.

The results showed that after about 10 days of feeding, Amblyseius pastereii could multiply rapidly, and the amount of Amblyseius pastereii increased about 2 to 10 times per week.

Example 4

1. Preparation of liquid feed

The preparation method of liquid feed is as follows:

S1. Add 15ml sterile water to 20 fly maggot larvae and homogenize in an electric homogenizer, then centrifuge the homogenate, and get 12ml supernatant for subsequent use;

S2. Get 5g of acarid mites and add 13ml of sterile water to homogenize in an electric homogenizer, then centrifuge the homogenate, and get 15ml of supernatant for subsequent use;

S3. Take 15ml of egg yolk for later use;

S4. Take 15ml of milk for later use;

S5. Take 5ml of honey for later use;

S6. After grinding the complex vitamin B and vitamin C into powder respectively, take 1.5g each according to the dose and place it in the container;

S7. Mix the components prepared in S1-S6 into the container of S6, add 2ml of sodium benzoate, make up the remaining amount to 100ml with sterile water, and stir well.

2. Make the liquid feed into small capsules with elongated wrapping materials (the above-mentioned wrapping materials are stretchable sealing film or stretchable plastic wrap) (the size of the small capsules is roughly 1-2cm, but not Therefore, the present invention is defined), placed in a culture device, and inserted into A. cucurbita mite for cultivation and breeding. The conditions for culturing and breeding are temperature 28°C and relative humidity 70%.

The results showed that after about 10 days of feeding, A. cucurbita could rapidly multiply, and the amount of A. cucurbita increased by about 2 to 10 times per week.

Example 5

1. Preparation of liquid feed

The preparation method of liquid feed is as follows:

S1. Add 15ml sterile water to 20 fly maggot larvae and homogenate in an electric homogenizer, then centrifuge the homogenate, and get 13ml supernatant for subsequent use;

S2. Get 5g of acarina and add 15ml of sterile water to homogenize in an electric homogenizer, then centrifuge the homogenate, and get 13ml of supernatant for subsequent use;

S3. Take 10ml of egg yolk for later use;

S4. Take 10ml of milk for later use;

S5. Take 5ml of honey for later use;

S6. After grinding the complex vitamin B and vitamin C into powder respectively, take 1.5g each according to the dose and place it in the container;

S7. Mix the components prepared in S1-S6 into the container of S6, add 2.5ml of propylparaben, make up the remaining amount to 100ml with sterile water, and stir well.

2. Make the liquid feed into small capsules with elongated wrapping material (the wrapping material is stretchable sealing film or stretchable plastic wrap) (the size of the small capsule is roughly 1-2cm, but not Therefore, the present invention is defined), placed in a culture device, and inserted into Neoseiulus swirskii for culture and breeding. The conditions for cultivation and breeding are temperature 24°C and relative humidity 70%.

The results showed that after about 10 days of feeding, Amblyseius reckeii could multiply rapidly, and the amount of Amblyseius reckeii increased by about 2 to 10 times per week.

Example 6

1. Preparation of liquid feed

The preparation method of liquid feed is as follows:

S1. Add 15ml sterile water to 20 fly maggot larvae and homogenate in an electric homogenizer, then centrifuge the homogenate, and get 10ml supernatant for subsequent use;

S2. Get 5g of acarina and add 15ml of sterile water to homogenate in an electric homogenizer, then centrifuge the homogenate, and get 15ml of supernatant for subsequent use;

S3. Take 11ml of egg yolk for later use;

S4. Take 11ml of milk for later use;

S5. Take 5ml of honey for later use;

S6. After grinding the complex vitamin B and vitamin C into powder respectively, take 1g each according to the dose and place it in the container;

S7. Mix the components prepared in S1-S6 into the container of S6, add 1ml of sodium dehydroacetate, make up the remaining amount to 100ml with sterile water, and stir well.

2. Make the liquid feed into small capsules with elongated wrapping material (the wrapping material is stretchable sealing film or stretchable plastic wrap) (the size of the small capsule is roughly 1-2cm, but not Therefore, the present invention is defined), placed in a culture device, and inserted into Amblyseius swirskii for culture and breeding. The conditions for culturing and breeding are temperature 25°C and relative humidity 60%.

The results showed that after about 10 days of feeding, Amblyseius sternii could multiply rapidly, and the amount of A. sternii increased by about 2 to 10 times per week.

Claims (5)

1. A liquid feed for raising phytoseiidae predatory mites, characterized in that, said liquid feed includes component vitamin B5～15mg/mL, vitamin C5～15mg/mL and the following volume percentage content Components: egg yolk 10%~20%, milk 10%~20%, honey 2%~5%, fly maggot homogenate 10%~20%, acaroid mite homogenate 10%~20% and preservative 0.5%~ 2%, the balance is water; The fly maggot homogenate is that the fly maggot larvae are added into water to homogenate, the homogenate is centrifuged, and the supernatant is taken to obtain the fly maggot homogenate; Homogenize, centrifuge the homogenate, take the supernatant to obtain the acarina homogenate; The phytoseiidae predatory mite is A. cucurbita, A. pasterei, A. steerii or A. reinneri; The preservative is one or a combination of potassium sorbate, sodium benzoate, streptococcus lactis, natamycin, sodium dehydroacetate, propyl p-hydroxybenzoate or dehydroacetic acid. 2. The liquid feed for raising Phytoseiaceae predatory mites according to claim 1, wherein said liquid feed includes components of vitamin B5-10 mg/mL, vitamin C5-10 mg/mL and below Components in percent by volume: 10% to 15% of egg yolk, 10% to 15% of milk, 4% to 5% of honey, 10% to 15% of fly maggot homogenate, 10% to 15% of acaroid mite homogenate and Preservative 0.5% ~ 1%, the balance is water. 3. The liquid feed for raising the phytoseiidae predatory mite according to claim 1 or 2, characterized in that, the phytoseiidae predatory mite is A. cucurbita or A. pastereii. 4. the liquid feed for raising Phytoseiidae predator mite according to claim 1 and 2, is characterized in that, described preservative is potassium sorbate, sodium benzoate or propyl p-hydroxybenzoate one or a combination of several. 5. according to claim 1 and 2, be used for raising the liquid feed of Phytoseiaceae predator mite, it is characterized in that, the preparation method of this liquid feed comprises the steps: S1. Add the fly maggot larvae to water, homogenate, centrifuge the homogenate, and take the supernatant for subsequent use; Take acaroid mites and add water to homogenate, centrifuge the homogenate, and take the supernatant for later use; Weigh egg yolk, milk and honey in proportion, and set aside; The complex vitamin B and vitamin C are ground into powder respectively and set aside; S2. Mix the components prepared in S1 evenly, add preservatives, make up the remaining amount with water, and stir evenly to obtain the liquid feed.