CN103583517B - Attractant for luring and gathering dichocrocis punctiferalis and bait - Google Patents

Abstract

The invention discloses an attractant and a lure core for attracting the peach borer and belongs to the field of attractant preparation for the peach borer. The attractant is a mixture of compounds α-pinene, camphene, sabinene and β-pinene, all of which are volatile active compounds released from the field of the main host plant (chestnut) of the peach borer borer. The attractant is mixed with volatile active compounds that have the effect of attracting the peach moth, which can attract a large number of female moths and a small amount of male moths. It can not only be used for dynamic monitoring and control of the population of peach borer in chestnut , and economical and effective, no environmental pollution.

Description

A kind of attractant and lure core for attracting peach borer

technical field

The invention relates to an attractant for the peach borer, in particular to an attractant and a lure core for attracting the peach borer.

Background technique

The peach borer Conogethes punctiferalis (Guenée) belongs to the order Lepidoptera and Mothidae. Its host plants include more than 40 kinds of fruit trees, forest trees and crops such as chestnuts, peaches, and corns. It is an omnivorous and boring pest. Because the larvae eat into the fruit quickly after hatching, it is very difficult to control the larvae. However, the application of insect pheromones to control adults has the advantages of strong specificity, high efficiency, no pollution, and no harm to beneficial insects. It can meet agricultural needs. requirements for sustainable development. Therefore, the application of pheromones to monitor and control peach borer has become the focus of attention at home and abroad in recent years. However, relying solely on insect sex pheromones only has a luring effect on male moths, but has no luring effect on female moths seeking host plants to lay eggs. Therefore, the field luring effect is greatly reduced.

Contents of the invention

The technical problem to be solved by the present invention is to provide an attractant and lure core for attracting the peach borer moth, which can effectively attract the peach borer moth female moth, thereby solving the problem that the existing attractant has a poor attracting effect on the peach borer moth. question.

In order to solve the above-mentioned technical problems, the present invention provides an attractant for attracting peach moth moth, the attractant is formed by mixing compounds α-pinene, camphene, sabinene and β-pinene, and each compound is peach Volatile active compounds released from the borer borer's main host plant (chestnut) in the field.

The present invention also provides a lure core for attracting peach moth moth. The lure core uses a green rubber stopper as a carrier, injects the attractant of claim 1 or 2 into the carrier, and makes the attractant Infiltration into the lure formed inside the carrier.

The beneficial effects of the present invention are: the attractant is mixed with active compounds capable of attracting the peach borer moth, which can attract a large number of peach borer moths and a small amount of male moths, and can not only be applied to the chestnut borer moth population Occurrence dynamic monitoring and prevention, and economical and effective, no environmental pollution.

Description of drawings

In order to illustrate the technical solutions of the embodiments of the present invention more clearly, the drawings that need to be used in the description of the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present invention. Those of ordinary skill in the art can also obtain other drawings based on these drawings on the premise of not paying creative work.

Fig. 1 is the cumulative lure moth curve of the lure core carrying the attractant provided by the embodiment of the present invention over time;

Fig. 2 is a curve of the daily total moth-attracting amount of lure cores carrying the attractant provided by the embodiment of the present invention.

Detailed ways

The following clearly and completely describes the technical solutions in the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

Embodiment one

The embodiment of the present invention provides an attractant for attracting peach moth moth. The attractant is formed by mixing compounds α-pinene, camphene, sabinene and β-pinene, and the dosage of each compound is preferably: : α-pinene 28, camphene 9, sabinene 37, β-pinene 26; each compound is a volatile active compound released from the main host plant (chestnut) of the peach moth borer in the field.

Since the attractant is mixed with active compounds that have the effect of attracting moths, it can attract a large number of female moths and a small number of male moths. Economical and effective, no environmental pollution.

Embodiment two

This embodiment provides a lure core for attracting peach moth moth. The lure core uses a green rubber plug as a carrier, injects the attractant of Example 1 into the carrier, and makes the attractant infiltrate into the A lure formed inside a carrier. The preferred dosage of attractant in each lure is 120.0 μg.

In the above-mentioned lure, the green rubber stopper is used as a carrier, and the attractant of claim 1 or 2 is injected into the carrier, including:

Soak the carrier with absolute ethanol and wash it several times, and let it dry at room temperature to evaporate the ethanol;

After diluting the attractant in Example 1 with n-hexane, inject it into the groove of the carrier at a set dosage level through a micro-syringe, and then add 10.0 μL of petroleum ether to completely infiltrate the attractant into the inside the carrier.

The attractant of the present invention will be further described in detail in conjunction with the specific preparation and use process below.

First, the attractant is mixed with active volatile compounds α-pinene, camphene, sabinene and β-pinene in a mass ratio of 28:9:37:26;

Next, utilize the above-mentioned attractants to make lure cores:

Use the green rubber stopper as the carrier to make the lure, soak the carrier rubber stopper with absolute ethanol and wash it several times, let it dry at room temperature to make the ethanol evaporate, and then according to the set dose level (120.0μg/lure), After diluting the attractant with n-hexane, inject 10.0 μL into the groove of the rubber stopper through a micro-syringe, and immediately add 10.0 μL of petroleum ether to promote the complete infiltration of volatile odor compounds into the interior of the carrier.

Utilize above-mentioned lure core that makes to make trap:

The trap used in the test is a water basin trap. Take a small green plastic basin (inner diameter 25.0cm, depth 10.0cm), pour 0.5% washing powder aqueous solution prepared with clean water and washing powder into the basin, and put the prepared The lure core is fixed on the center of the water basin with a thin iron wire about 1.0 cm away from the water surface.

Use the above-mentioned attractant to carry out field moth luring test:

The field moth trap experiment was carried out at the Chestnut Germplasm Resource Station in Huairou District, Beijing from July 31, 2013 to September 16, 2013. The test site is located in Deshuiwan Town, Huairou District, Beijing. The chestnut orchard area is about 100hm ² . The main cultivated varieties are Yanhong, Huaihuang and Huaijiu. The average age of chestnut trees is nearly 30 years, and the average plant height is about 6.0m. According to the layout method of placing a trap at an interval of 15.0m, hang it on the branch 1.2-1.5m above the ground. The traps at different points are randomly placed, and the traps are re-arranged every 7 days to reduce the effect of hanging orientation on trapping. Impact. From the second day after hanging the lure core, check the moth situation regularly at 7:00 every morning, record the amount of lures and fish out the dead moths. Change the washing powder aqueous solution every 5 days, change the lure every 25 days, and observe continuously for 46 days. The commercial peach moth moth sex pheromone lure was used as a control, and 3 replicates were set for each treatment.

Results and Analysis:

Moth-attracting field trials with plant-sourced attractants:

Judging from the trapping effect in the field, the attractant of the present invention is quite different from the sex pheromone attractant of the commercial peach moth moth, and the 3 lure cores (that is, 3 repetitions) carrying the attractant of the embodiment of the present invention accumulate 46 days. The number of moths attracted reached 116, while the cumulative number of moths attracted by the three commercial sex pheromone lures was 31. There was a significant difference in the cumulative number of moths attracted by the two attractants.

From the results of monitoring the population dynamics of the chestnut borer moth by using plant-source attractants, the result of trapping with the attractant of the embodiment of the present invention shows that the occurrence of peach borer adults suddenly increased on August 24, 3 A total of 10 moths were lured per lure day, and there was a large occurrence peak on August 25, with a total of 45 moths, and a sudden decrease on August 26, when the number of moths was 15; , there was a small peak on September 10, and the total number of moths attracted by the three lures was 12 per day, while the total number of moths was lower at other times (0-4), and there were no traps for several consecutive days in mid-September To the peach borer adult (seeing Fig. 1, 2, Lpv in Fig. 1 and Fig. 2 are all lures carrying the attractant of the present invention, and Lck are commercial lures). The lure result of the commercialized peach moth moth lure is less than that of the attractant of the present invention, and it is consistent with the lure result of the lure of the embodiment of the present invention in terms of elucidating the population dynamics of the chestnut moth moth. , indicating that there will be a peak period of occurrence of peach borer adults in the Beijing area in mid-to-late August, and also proves that the attractant formulations of the embodiments of the present invention can be used to monitor the population dynamics and occurrence rules of chestnut borer moths.

In summary, the attractant of the present invention has a large amount of moth lures and no pollution to the environment. While being applied to the dynamic monitoring of the occurrence of the population of the peach borer in the chestnut garden, it can also be directly used for the prevention and treatment of the adult peach borer to solve the problem of larvae. The effect of chemical control is not ideal and causes environmental pollution and other problems. In order to improve the control effect, an average of 2 to 3 traps can be used per mu, and the trapped moths should be removed in time.

So far, there are only reports on the formula of sex pheromone attractant of peach borer moth in the world, and there is no report on the use of host plant volatiles as attractant. The applicant found that the attractant of the present invention is mixed with the volatile active compounds α-pinene, camphene, sabinene and β-pinene of the host plant of peach borer borer (chestnut) according to the mass ratio of 28:9:37:26 , when trapping at a dose of 120.0 μg/lure, a large number of female moths and a small number of male moths can be lured. pollute. For the first time in my country, the present invention uses the headspace dynamic gas sampling method to extract and identify the volatile odor compounds of the host plant of the peach moth borer-chestnut fruiting branches, and selects the volatile active compounds that have an attractive effect on the peach borer moth adults to prepare An attractant, the attractant can not only effectively attract a large number of female moths and a small number of male moths, but also has an important role and significance for the dynamic monitoring and control of the population dynamics of the peach borer in chestnut orchards.

The above is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any person familiar with the technical field can easily conceive of changes or changes within the technical scope disclosed in the present invention. Replacement should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention should be determined by the protection scope of the claims.

Claims (4)

1. lure an attractant for collection dichocrocis punctiferalis, it is characterized in that, this attractant is formed by compound australene, amphene, sabinene and nopinene mixture;

Described each compound amount is in mass ratio: australene: amphene: sabinene: nopinene=28:9:37:26.

2. attractant as claimed in claim 1, it is characterized in that, described compound australene, amphene, sabinene and nopinene are the volatile active compound extracted from dichocrocis punctiferalis main host plants plant Chinese chestnut.

3. what lure collection dichocrocis punctiferalis lures a core, and it is characterized in that, this lures core to be with green rubber plug for carrier, and in described carrier, injection adds the attractant of described claim 1 or 2, and described attractant is infiltrated enter described carrier inside to be formed to lure core.

4. lure core as claimed in claim 3, it is characterized in that, described with green rubber plug for carrier, in described carrier, injection adds the attractant of described claim 1 or 2, comprising:

Described carrier soaked in absolute ethyl alcohol is cleaned for several times, dries in the air under room temperature and put that ethanol volatilized is clean;

After the attractant n-hexane dilution described in described claim 1 or 2, be injected in the groove of described carrier by micro syringe by the dosage level of setting, add 10.0 μ L benzinums afterwards and make described attractant complete wetting enter described carrier inside;

The dosage of described setting is each attractant luring core to add 120.0 μ g.