CN103583236A - Method for preparing fleckedflesh polypore mycelium - Google Patents
Method for preparing fleckedflesh polypore mycelium Download PDFInfo
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- CN103583236A CN103583236A CN201310573715.1A CN201310573715A CN103583236A CN 103583236 A CN103583236 A CN 103583236A CN 201310573715 A CN201310573715 A CN 201310573715A CN 103583236 A CN103583236 A CN 103583236A
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- fleckedflesh
- polypore
- mycelium
- phellinus
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- 238000000034 method Methods 0.000 title abstract description 15
- 235000013312 flour Nutrition 0.000 claims abstract description 16
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 8
- 240000008042 Zea mays Species 0.000 claims abstract description 8
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 8
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 8
- 235000005822 corn Nutrition 0.000 claims abstract description 8
- 238000002360 preparation method Methods 0.000 claims abstract description 8
- 235000009566 rice Nutrition 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 238000011081 inoculation Methods 0.000 claims abstract description 6
- 240000007594 Oryza sativa Species 0.000 claims abstract 3
- 241000123107 Phellinus Species 0.000 claims description 32
- 239000002994 raw material Substances 0.000 claims description 16
- 241000123113 Phellinus igniarius Species 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 9
- 235000015099 wheat brans Nutrition 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000007796 conventional method Methods 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000006188 syrup Substances 0.000 claims description 3
- 235000020357 syrup Nutrition 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 12
- 230000008901 benefit Effects 0.000 abstract description 5
- 239000000654 additive Substances 0.000 abstract description 3
- 230000000996 additive effect Effects 0.000 abstract description 3
- 235000013376 functional food Nutrition 0.000 abstract description 3
- 239000000047 product Substances 0.000 abstract 3
- 239000012467 final product Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 235000013399 edible fruits Nutrition 0.000 description 7
- 239000003814 drug Substances 0.000 description 6
- 241000209094 Oryza Species 0.000 description 5
- 230000001093 anti-cancer Effects 0.000 description 5
- 230000007812 deficiency Effects 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 244000144730 Amygdalus persica Species 0.000 description 1
- 235000018185 Betula X alpestris Nutrition 0.000 description 1
- 235000018212 Betula X uliginosa Nutrition 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 241000124033 Salix Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000002300 anti-fibrosis Effects 0.000 description 1
- 230000000118 anti-neoplastic effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a method for preparing fleckedflesh polypore mycelium. The method specifically comprises the steps that a culture medium composed of rice bran, bran, corn flour, flour and brown sugar is provided, fleckedflesh polypore liquid spawn is used as spawn, the inoculation method and the inoculation amount of the liquid spawn are well grasped, and the fleckedflesh polypore mycelium is prepared through the reasonable arrangement of a production environment. By the adoption of the method for preparing the fleckedflesh polypore mycelium, the application problems that medicinal fleckedflesh polypore resources are influenced due to the fact that wide fleckedflesh polypore resources are insufficient and a large amount of fleckedflesh polypore cannot be produced due to the large difficulty of manual cultivation are solved. The method for preparing the fleckedflesh polypore mycelium has the advantages that the cultivation period is short, the production preparation technology is simple, the production cost of the product is low, and the production efficiency of the product is high and stable. In addition, the fleckedflesh polypore mycelium as the final product is used as an additive which is widely applied to the fields of pharmacy, biological health care products, special drinks, functional foods and the like and is good in market prospect and remarkable in benefit.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of preparation method of phellinus igniarius mycelium.
Technical background
Phellinus is rare medicinal fungi, and its fruit body can be used as medicine.Its main parasitic is on the snags of the broad-leaved trees such as poplar, willow, birch, peach and standing tree and on trunk, and its major function is anticancer, and anti-fibrosis is anti-oxidant, and its treatment the efficient of cancer reaches more than 90%.Phellinus in biological anticancer field by internationally recognized be the fungi of tool anticancer function, it is the international the world of medicine of the efficient ，Shi that ranks the first and health products trade antineoplastic product raw materials for production in anticancer field.The Chinese medicine that this taste is ancient, reputation can not show a candle to Cordyceps sinensis, but its effect is but poor unlike Cordyceps sinensis.
At present, internationally recognized biological Phellinus polysaccharide is to extract and have bioactive polysaccharose substance mycelium from Phellinus, fruit body.Studies confirm that, Phellinus polysaccharide have obvious immunological regulation, anticancer antitumor, anti-oxidant, reduce the effects such as blood sugar, protection liver.
At present, the factor that is restricting the further development and utilization of Phellinus mainly contains: 1, Phellinus wild resource is day by day deficient, and wild Phellinus resource is difficult to recover, and causes Phellinus fruit body to form in a large number; 2, Phellinus is to environment sensitive, and vegetative period is long, and artificial cultivation difficulty is larger.
In order to have solved because the deficiency of wild Phellinus resource and artificial cultivation difficulty cause greatly Phellinus fruit body in a large number output then affect the application problem in Phellinus medicine source; through scientific research personnel, constantly study, now by biological submerged fermentation technological development, go out brand-new a kind of Phellinus product---phellinus igniarius mycelium.
Research shows, the active ingredient of phellinus igniarius mycelium is the same with fruit body.By biological submerged fermentation technology, produce phellinus igniarius mycelium, the feature such as it is with short production cycle, technique is simple, production efficiency is high and stable, is the main development direction of manually cultivating Phellinus product.
Summary of the invention
Object of the present invention is just to provide the preparation method of brand-new a kind of phellinus igniarius mycelium.Specifically comprise: the medium being formed by rice bran, wheat bran, corn flour, flour, brown sugar, with Phellinus liquid spawn, as bacterial classification, get hold of inoculation method and the inoculum concentration of liquid spawn, by the environment of reasonably arranging production, phellinus igniarius mycelium is cultivated out.By implementing the present invention, effectively solved because the deficiency of wild Phellinus resource and artificial cultivation difficulty cause greatly Phellinus fruit body in a large number output then affect the application problem in Phellinus medicine source.Meanwhile, the process for producing of final products---phellinus igniarius mycelium, has the features such as cultivation period is short, process for producing simple, the production cost production efficiency low, product of product is high and stable.In addition, implement the present invention, final products---phellinus igniarius mycelium, is widely used in the fields such as pharmacy, biological health-care product, characteristic drink, functional food as a kind of additive, and market prospects are good, remarkable benefit.
The present invention is achieved through the following technical solutions:
A preparation method for phellinus igniarius mycelium, comprises the steps: in technical scheme
1, prepare medium: the raw material of medium is by 60% rice bran, 22% wheat bran, 11% corn flour, 5% flour, 2% brown sugar forms, first by rice bran, wheat bran, corn flour and flour mix and stir and mixed material, brown sugar is diluted in appropriate clear water again, then mixed material and syrup are mixed and stirred, and the water content of mixed material is adjusted into 50～55% must produces with raw material, then raw material is sub-packed in culture vessel, thickness when raw material packs culture vessel into is 5～6 centimetres, finally the culture vessel that raw material is housed is sealed and sterilization according to a conventional method.
2, inoculation: under gnotobasis, cultured Phellinus liquid spawn is transferred in culture matrix.
3, cultural hypha: the culture vessel connecting kind being moved on to culturing room's lucifuge and cultivate, is to cultivate 4～5 days under 26～29 ℃, the relative moisture environment that is 60～65% in temperature, and medium covers with phellinus liteus.
4, finished product: when phellinus liteus covers with medium, medium is obtained to finished product mycelium by low temperature drying to moisture content in medium lower than 8%.
The present invention both had the following advantages:
1, phellinus liteus preparation technique proposed by the invention is simple, easily implements.
2, by implementing the present invention, effectively solved because the deficiency of wild Phellinus resource and artificial cultivation difficulty cause greatly Phellinus fruit body in a large number output then affect the application problem in Phellinus medicine source.
3,, by implementing the present invention, the process for producing of final products---phellinus igniarius mycelium, has the features such as cultivation period is short, process for producing simple, the production cost production efficiency low, product of product is high and stable.
4, by implementing the present invention, final products---phellinus igniarius mycelium, is widely used in the fields such as pharmacy, biological health-care product, characteristic drink, functional food as a kind of additive, and market prospects are good, remarkable benefit.
Embodiment
Below in conjunction with embodiment, method of the present invention is further illustrated.
A preparation method for phellinus igniarius mycelium, specific implementation method is as follows:
1, prepare medium: the raw material of medium is by 60% rice bran, 22% wheat bran, 11% corn flour, 5% flour, 2% brown sugar forms, first by rice bran, wheat bran, corn flour and flour mix and stir and mixed material, brown sugar is diluted in appropriate clear water again, then mixed material and syrup are mixed and stirred, and the water content of mixed material is adjusted into 50～55% must produces with raw material, then raw material is sub-packed in culture vessel, thickness when raw material packs culture vessel into is 5～6 centimetres, finally the culture vessel that raw material is housed is sealed and sterilization according to a conventional method.
2, inoculation: under gnotobasis, cultured Phellinus liquid spawn is transferred in culture matrix.
3, cultural hypha: the culture vessel connecting kind being moved on to culturing room's lucifuge and cultivate, is to cultivate 4～5 days under 26～29 ℃, the relative moisture environment that is 60～65% in temperature, and medium covers with phellinus liteus.
4, finished product: when phellinus liteus covers with medium, medium is obtained to finished product mycelium by low temperature drying to moisture content in medium lower than 8%.
Claims (1)
1. a preparation method for phellinus igniarius mycelium, it is to comprise the steps:
(1) prepare medium: the raw material of medium is by 60% rice bran, 22% wheat bran, 11% corn flour, 5% flour, 2% brown sugar forms, first by rice bran, wheat bran, corn flour and flour mix and stir and mixed material, brown sugar is diluted in appropriate clear water again, then mixed material and syrup are mixed and stirred, and the water content of mixed material is adjusted into 50～55% must produces with raw material, then raw material is sub-packed in culture vessel, thickness when raw material packs culture vessel into is 5～6 centimetres, finally the culture vessel that raw material is housed is sealed and sterilization according to a conventional method,
(2) inoculation: under gnotobasis, cultured Phellinus liquid spawn is transferred in culture matrix;
(3) cultural hypha: the culture vessel connecting kind being moved on to culturing room's lucifuge and cultivate, is to cultivate 4～5 days under 26～29 ℃, the relative moisture environment that is 60～65% in temperature, and medium covers with phellinus liteus;
(4) finished product: when phellinus liteus covers with medium, medium is obtained to finished product mycelium by low temperature drying to moisture content in medium lower than 8%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310573715.1A CN103583236A (en) | 2013-11-16 | 2013-11-16 | Method for preparing fleckedflesh polypore mycelium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310573715.1A CN103583236A (en) | 2013-11-16 | 2013-11-16 | Method for preparing fleckedflesh polypore mycelium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103583236A true CN103583236A (en) | 2014-02-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310573715.1A Pending CN103583236A (en) | 2013-11-16 | 2013-11-16 | Method for preparing fleckedflesh polypore mycelium |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105053991A (en) * | 2015-08-18 | 2015-11-18 | 雷色香 | Lentinus edodes mycelium powder |
| CN105900696A (en) * | 2016-06-28 | 2016-08-31 | 黄秀英 | Phellinus igniarius mycelium prepared from dendrobium officinale as culture medium |
| CN106047721A (en) * | 2016-06-28 | 2016-10-26 | 黄秀英 | Phellinus igniarius mycelium with functions of dampness elimination, slimming and health care |
| CN106047723A (en) * | 2016-06-28 | 2016-10-26 | 黄秀英 | Preparation of phellinus linteus mycelia by taking red dates and wolfberry fruits as culture medium |
| CN106072493A (en) * | 2016-06-28 | 2016-11-09 | 黄秀英 | Thallus Laminariae (Thallus Eckloniae) phellinus igniarius mycelium is prepared for culture medium with Thallus Laminariae (Thallus Eckloniae) |
| CN106107943A (en) * | 2016-06-28 | 2016-11-16 | 黄秀英 | Phellinus liteus block is prepared for culture medium with Fructus Jujubae and Fructus Lycii |
| CN106119127A (en) * | 2016-06-28 | 2016-11-16 | 黄秀英 | One is prepared Phellinus igniarius (L. ex Fr.) Quel. with Moringa and mulberry for raw material and is combined mycelial method |
| CN106107944A (en) * | 2016-06-28 | 2016-11-16 | 黄秀英 | With pitaya peel for a kind of phellinus igniarius mycelium of culture medium preparation |
| CN107182544A (en) * | 2016-10-27 | 2017-09-22 | 镇江市农业科学技术实业公司 | A kind of Phellinus fructification rough machining method |
-
2013
- 2013-11-16 CN CN201310573715.1A patent/CN103583236A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105053991A (en) * | 2015-08-18 | 2015-11-18 | 雷色香 | Lentinus edodes mycelium powder |
| CN105900696A (en) * | 2016-06-28 | 2016-08-31 | 黄秀英 | Phellinus igniarius mycelium prepared from dendrobium officinale as culture medium |
| CN106047721A (en) * | 2016-06-28 | 2016-10-26 | 黄秀英 | Phellinus igniarius mycelium with functions of dampness elimination, slimming and health care |
| CN106047723A (en) * | 2016-06-28 | 2016-10-26 | 黄秀英 | Preparation of phellinus linteus mycelia by taking red dates and wolfberry fruits as culture medium |
| CN106072493A (en) * | 2016-06-28 | 2016-11-09 | 黄秀英 | Thallus Laminariae (Thallus Eckloniae) phellinus igniarius mycelium is prepared for culture medium with Thallus Laminariae (Thallus Eckloniae) |
| CN106107943A (en) * | 2016-06-28 | 2016-11-16 | 黄秀英 | Phellinus liteus block is prepared for culture medium with Fructus Jujubae and Fructus Lycii |
| CN106119127A (en) * | 2016-06-28 | 2016-11-16 | 黄秀英 | One is prepared Phellinus igniarius (L. ex Fr.) Quel. with Moringa and mulberry for raw material and is combined mycelial method |
| CN106107944A (en) * | 2016-06-28 | 2016-11-16 | 黄秀英 | With pitaya peel for a kind of phellinus igniarius mycelium of culture medium preparation |
| CN105900696B (en) * | 2016-06-28 | 2019-03-01 | 广西壮仁堂生物科技有限公司 | Phellinus igniarius mycelium is prepared by culture medium of dendrobium candidum |
| CN107182544A (en) * | 2016-10-27 | 2017-09-22 | 镇江市农业科学技术实业公司 | A kind of Phellinus fructification rough machining method |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140219 |
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| WD01 | Invention patent application deemed withdrawn after publication |