CN103518947A

CN103518947A - Production method of biological protein feed

Info

Publication number: CN103518947A
Application number: CN201210234300.7A
Authority: CN
Inventors: 王芃
Original assignee: Individual
Current assignee: Wang Anzhen
Priority date: 2012-07-07
Filing date: 2012-07-07
Publication date: 2014-01-22

Abstract

The invention discloses a production method of a biological protein feed, and the method is a technological method for producing a mycoprotein feed through solid state aerobic fermentation. The production method of the biological protein feed comprises the following steps of drying brewed residues, adding auxiliary materials, and adjusting the acidity; inoculating a liquid fungi culture, introducing into an air ventilation tank for fermenting and culturing, drying fermented culture materials timely, removing hard shells, crushing and packaging screened materials, so as to obtain the biological protein feed. According to the feed, the content of coarse protein is more than 33%, and the content of coarse fiber is less than 14%.

Description

A kind of production method of biological protein feedstuff

Technical field

The invention discloses and a kind ofly take spirit stillage as primary raw material, inoculation liquid mould bacterial classification, through the production method of producing vinasse somatic protein fodder by solide aerobic fermentation.

Background technology

China is as a wine brewing big country, and vinasse resource is very abundant.According to statistics, generation is in 7,000,000 tons of left and right (in dry) vinasse year.The utilization rate of vinasse is not high at present, the restraining factors that restriction distillers ' grains resource makes full use of have two: the one, and bright poor acidity large (PH is about 4), moisture high (60%-65%), easily putrid and deteriorated, do not utilize and preserve and way far away transportation, can only use in locality, the 2nd, because white wine has added a large amount of rice husks in alcoholic process in wine unstrained spirits.After measured, in air-dry thing, the weight of rice husk accounts for 30% left and right, and volume accounts for 50% left and right, and coarse-fibred content reaches more than 18.6%, as feed digestibility low, palatability is poor, is difficult to absorb, and affects feed efficiency.Traditionally, vinasse are mainly directly used as feed filler by near peasant brewery and raiser, the mode of this extensive using, and benefit is very low, is a kind of wasting of resources to a great extent.Also have and after vinasse are dried, remove rice husk to pulverize to do feed, but this method added value of product is low, and lower as feed nutritive value.

Summary of the invention

The invention provides a kind of process that spirit stillage is converted into high protein feed.First vinasse are carried out to predrainage, and add therein a small amount of auxiliary material, then inoculate geotrichum candidum liquid spawn and carry out fermentation process, produce vinasse animal feeding-stuff containing somatic protein, can increase substantially the nutritive value of vinasse like this, increase its added value, and be convenient to storage and way far away transportation, there is good economic results in society and environment protection significance.

The process that vinasse animal feeding-stuff containing somatic protein monk produces is as follows:

(1) fresh grain stillage dehydration pretreatment

The aquatic foods grain that is 60-65% left and right by moisture content is dried to the about 40%-50% of moisture content, treats nutrition-allocated proportion and fermented and cultured use.

(2) vinasse compost fermented pretreatment

Because vinasse are liquor-making byproduct, content of reducing sugar is lower, and the carbon source that microorganism easily utilizes is less.Therefore the wheat bran that adds about 10%-15% in the grain material of pretreatment dehydration is carbon source as a supplement.

Vinasse acidity is larger, and pH value is less than 4, and this is unfavorable to normal fermentation process, therefore add ammoniacal liquor in the mixture of vinasse and wheat bran, regulates pH value to 5.5-6.5.Simultaneously in order to improve the protein content in tunning, therefore the ammonium sulfate that adds 5%-7% as nitrogenous source, regulates compost moisture to moisture content 60%-65%.

(3) production of hybrid seeds

Bacterial classification used in this technique is edible geotrichum candidum, adopts the mode of the liquid production of hybrid seeds.Order according to inclined-plane kind activation → one-level shaking flask kind → secondary shaking flask kind → seedlet tank → large seeding tank is carried out the production of hybrid seeds.

(4) inoculation, fermentation

Compost after nutrition-allocated proportion is inoculated to above-mentioned geotrichum candidum liquid spawn, inoculum concentration is 5%-10%, fully mix rear top fermentation bed flat (fermenting cellar was sterilized with sulphur flue gas every a couple of days) thoroughly, fermentation layer thickness is 20-30 centimetre, fermentation material temperature remains on 28-32 ℃ of scope, aerlbic culture, fermentation 22-30 hour.When winter, ambient temperature was low, need heating to do temperature-compensating.

(5) dry, go rice husk, screenings to be pulverized and packing

The compost fermenting contains a certain amount of moisture, therefore need drying and dehydrating to moisture content 12%-13% left and right.Dried material is rubbed with cone mill, then uses vibrosieve from rice husk, and screenings is pulverized, and makes the vinasse animal feeding-stuff containing somatic protein finished product of taste giving off a strong fragrance after packing.

The specific embodiment

Embodiment:

By 1.8 tons of fresh grain stillage drying and dewaterings to moisture content 40% left and right, and add 100 kilograms of wheat brans, mix, at the mixed liquor of admixing the ammoniacal liquor of 380 kilograms of running water, 60 kilograms of ammonium sulfate and 50 kilogram of 18% left and right, regulate material pH value to 6 left and right, place stack retting 1 hour.

Fermenting cellar is sterilized with sulfur fumigation, vented exhaust, then the fermentation medium for the treatment of of stack retting being crossed is inoculated geotrichum candidum liquid spawn, inoculum concentration is 5% of dry grain, fully mix rear upper cement ventilating fermentation pond (fermentation bed area 2.4*5.2 square metre) thoroughly and pave, compost temperature remains on 28-32 ℃ of left and right, batch (-type) ventilation in first 8 hours, lead to afterwards wind and cultivate about 22 hours, now compost becomes penetrating white thing.After cultured fermentation material is dry 120 ℃ of left and right, with cone mill, rub, vibrosieve is left away except rice husk, and screenings is pulverized and obtained the vinasse animal feeding-stuff containing somatic protein finished product that crude protein content is more than 33%, crude fiber content is less than 14%.

Claims

1. a production method for biological protein feedstuff, is characterized in that: will brewage after slag oven dry, and add auxiliary material and regulate acidity, inoculation liquid mould bacterial classification, the pond fermented and cultured that enters to ventilate, duricrust, screenings crushing packing are dried, gone to the compost having fermented in time, both bioprotein beverage.

2. the production method of a kind of biological protein feedstuff according to claim 1, is further characterized in that: before fermentation the drying of slag dewater to moisture content be 45%50%, treat that nutrition-allocated proportion and fermented and cultured use.

3. the production method of a kind of biological protein feedstuff according to claim 1, is further characterized in that: nutrition-allocated proportion is: slag accounts for 70-80%; Wheat bran accounts for 10---and 15%; Ammonium sulfate accounts for 5---and 7%; Ammoniacal liquor accounts for 3---and 8%.