CN103430962A - Bacillus subtilis and kresoxim-methyl composition - Google Patents
Bacillus subtilis and kresoxim-methyl composition Download PDFInfo
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- CN103430962A CN103430962A CN2013103584503A CN201310358450A CN103430962A CN 103430962 A CN103430962 A CN 103430962A CN 2013103584503 A CN2013103584503 A CN 2013103584503A CN 201310358450 A CN201310358450 A CN 201310358450A CN 103430962 A CN103430962 A CN 103430962A
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- kresoxim
- methyl
- bacillus subtilis
- bacillus
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- 244000063299 Bacillus subtilis Species 0.000 title claims abstract description 28
- 235000014469 Bacillus subtilis Nutrition 0.000 title claims abstract description 28
- 239000005800 Kresoxim-methyl Substances 0.000 title claims abstract description 23
- ZOTBXTZVPHCKPN-HTXNQAPBSA-N kresoxim-methyl Chemical compound CO\N=C(\C(=O)OC)C1=CC=CC=C1COC1=CC=CC=C1C ZOTBXTZVPHCKPN-HTXNQAPBSA-N 0.000 title claims abstract description 23
- 239000000203 mixture Substances 0.000 title claims abstract description 19
- 239000004480 active ingredient Substances 0.000 claims abstract description 6
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract 7
- 239000004563 wettable powder Substances 0.000 claims description 15
- 239000000839 emulsion Substances 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 239000012752 auxiliary agent Substances 0.000 claims 1
- 239000000725 suspension Substances 0.000 claims 1
- 235000007164 Oryza sativa Nutrition 0.000 abstract description 6
- 235000009566 rice Nutrition 0.000 abstract description 6
- 241000221785 Erysiphales Species 0.000 abstract description 4
- 241000221662 Sclerotinia Species 0.000 abstract description 4
- 241000209140 Triticum Species 0.000 abstract description 4
- 235000021307 Triticum Nutrition 0.000 abstract description 4
- 230000002265 prevention Effects 0.000 abstract description 4
- 230000003032 phytopathogenic effect Effects 0.000 abstract description 3
- 230000002195 synergetic effect Effects 0.000 abstract description 3
- 241000894006 Bacteria Species 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 abstract description 2
- 241001530056 Athelia rolfsii Species 0.000 abstract 1
- 235000006008 Brassica napus var napus Nutrition 0.000 abstract 1
- 240000000385 Brassica napus var. napus Species 0.000 abstract 1
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 5
- 238000009472 formulation Methods 0.000 description 4
- 239000000575 pesticide Substances 0.000 description 4
- 244000052616 bacterial pathogen Species 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 240000002791 Brassica napus Species 0.000 description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000000855 fungicidal effect Effects 0.000 description 2
- 239000000417 fungicide Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000003449 preventive effect Effects 0.000 description 2
- 241000235349 Ascomycota Species 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- 241000186146 Brevibacterium Species 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 244000000005 bacterial plant pathogen Species 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000007884 disintegrant Substances 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
一、技术领域1. Technical field
本发明属于农药领域，具体涉及一种含枯草芽孢杆菌(Bacillus subtilis)和醚菌酯的组合物。The invention belongs to the field of pesticides, in particular to a composition containing Bacillus subtilis and kresoxim-methyl.
二、背景技术2. Background technology
枯草芽孢杆菌，属生物杀菌剂，该菌从土壤中分离得到，短杆菌属。专用于包衣处理水稻种子，具有激活作物生长、减轻水稻纹枯病、细菌性条斑病、白叶枯病、等病菌危害的作用。通过竞争性生长繁殖占居生存空间的方式来阻止植物病原真菌的生长，能在作物表面迅速形成保护膜，使植物病原菌得不到生存空间，从而保护了农作物免受病原菌危害。枯草芽孢杆菌可分泌抑菌物质，抑制病原菌孢子萌芽和菌丝生长，从而达到预防与治疗目的。Bacillus subtilis is a biological fungicide, which is isolated from soil and belongs to the genus Brevibacterium. It is specially used for coating rice seeds, which has the functions of activating crop growth and reducing the damage of rice sheath blight, bacterial streak disease, bacterial blight, and other pathogens. Preventing the growth of phytopathogenic fungi by competing for growth and reproduction to occupy the living space can quickly form a protective film on the surface of crops, preventing phytopathogenic bacteria from obtaining living space, thereby protecting crops from pathogenic bacteria. Bacillus subtilis can secrete antibacterial substances to inhibit the germination of pathogenic bacteria spores and the growth of hyphae, so as to achieve the purpose of prevention and treatment.
醚菌酯是一种低毒、高效、广谱的内吸性杀菌剂，具有良好的预防、治疗作用和持效期；可以与其他农药相混用；用于防治某些子囊菌、半知菌和担子菌纲真菌引起的病害，药效持效期长，对植物安全。Kresoxim-methyl is a low-toxic, high-efficiency, broad-spectrum systemic fungicide, which has good preventive and therapeutic effects and a long-lasting effect; it can be mixed with other pesticides; it can be used to control some ascomycetes and half-knowledge fungi and diseases caused by basidiomycete fungi, the drug effect lasts for a long time and is safe for plants.
三、发明内容3. Contents of the invention
本发明的目的是提供一种对多种农作物的病害均有防治作用的、以枯草芽孢杆菌和醚菌酯为主要活性物质的组合物。该组合物敬爱将减轻现有病原菌对醚菌酯的潜在抗药性风险。研究结果表明枯草芽孢杆菌或与醚菌酯的组合具有明显的协同作用。The object of the present invention is to provide a composition with bacillus subtilis and kresoxim-methyl as the main active substances, which has control effect on various crop diseases. The composition will reduce the risk of potential drug resistance of existing pathogenic bacteria to kresoxim-methyl. The results of the study showed that Bacillus subtilis or the combination with kresoxim-methyl had obvious synergistic effect.
本发明的目的可以通过以下措施达到：The purpose of the present invention can be achieved through the following measures:
一种含枯草芽孢杆菌和醚菌酯的组合物，该组合物以枯草芽孢杆菌和醚菌酯为活性成分，其质量比为1000∶1～1∶1000；枯草芽孢杆菌和醚菌酯的质量比优选为100∶1～1∶1000，更优选100∶1～1∶100，进一步优选为30∶1～1∶100，再进一步优选为30∶1～1∶30，更进一步优选为10∶1～1∶30，最优选10∶1～1∶10。本发明所采用的枯草芽孢杆菌中活孢子数为0.1亿/克～1000亿/克，优选为0.5亿/克～500亿/克，进一步优选为1亿/克～100亿/克。A composition containing Bacillus subtilis and Kresstrobin, the composition uses Bacillus subtilis and Kresoxim as active ingredients, and its mass ratio is 1000:1 to 1:1000; the mass of Bacillus subtilis and Kresoxim The ratio is preferably 100:1 to 1:1000, more preferably 100:1 to 1:100, even more preferably 30:1 to 1:100, still more preferably 30:1 to 1:30, and still more preferably 10:1 1 to 1:30, most preferably 10:1 to 1:10. The number of viable spores in the Bacillus subtilis used in the present invention is 0.1 billion/g to 100 billion/g, preferably 0.5 billion/g to 50 billion/g, more preferably 100 million/g to 10 billion/g.
向活性成分枯草芽孢杆菌和醚菌酯中加入农药助剂后即可配得可湿性粉剂、水悬浮剂或水乳剂。如向活性成分枯草芽孢杆菌和醚菌酯中加入助剂并调节PH值5～7后，配成可湿性粉剂。醚菌酯可以采用原药，枯草芽孢杆菌采用原粉。农药助剂可以选用湿润剂、分散剂、填料、粘结剂、崩解剂等中一种或几种。Wettable powder, water suspension or water emulsion can be prepared after adding pesticide adjuvants to the active ingredients Bacillus subtilis and kresoxim-methyl. For example, after adding additives to the active ingredients Bacillus subtilis and kresoxim-methyl and adjusting the pH value to 5-7, it can be made into a wettable powder. Kresoxim-methyl can use the original drug, and Bacillus subtilis can use the original powder. Pesticide auxiliaries can be selected from one or more of wetting agents, dispersants, fillers, binders, disintegrants, etc.
本发明的组合物降低了现有植物病原菌对醚菌酯和枯草芽孢杆菌的潜在抗药性风险；枯草芽孢杆菌与醚菌酯组合具有明显的协同作用，同时又减少了用药量，从而使该组合物可以广泛应用于防治油菜菌核病、水稻纹枯病、小麦白粉病等。The composition of the present invention reduces the risk of potential drug resistance of existing plant pathogenic bacteria to kresoxim-methyl and Bacillus subtilis; the combination of Bacillus subtilis and kresoxim-methyl has obvious synergistic effect, and at the same time reduces the dosage, so that the combination It can be widely used in the control of rapeseed sclerotinia, rice sheath blight, wheat powdery mildew and so on.
四、具体实施方式4. Specific implementation
制剂实施例1：22％可湿性粉剂(各组份为质量份)Formulation Example 1: 22% wettable powder (each component is parts by mass)
制剂实施例2：33％可湿性粉剂(各组份为质量份)Formulation Example 2: 33% wettable powder (each component is parts by mass)
制剂实施例3：60％可湿性粉剂(各组份为质量份)Formulation Example 3: 60% wettable powder (each component is parts by mass)
制剂实施例4：80％可湿性粉剂(各组份为质量份)Formulation Example 4: 80% wettable powder (each component is parts by mass)
药效实施例1Efficacy Example 1
将枯草芽孢杆菌(200亿活孢子/克)与醚菌酯按1∶1(质量比)的比例配成22％可湿性粉剂，喷雾，应用于防治油菜菌核病，单独将枯草芽孢杆菌和醚菌酯配成上述可湿性粉制作对比，结果见表1。Bacillus subtilis (20,000,000,000 living spores/gram) and kresoxim-methyl are made into 22% wettable powder in the ratio of 1: 1 (mass ratio), sprayed, and are applied to the prevention and treatment of rapeseed sclerotinia, separately Bacillus subtilis and Kresoxim-methyl was formulated into the above wettable powder for comparison, and the results are shown in Table 1.
表122％枯草芽孢杆菌·醚菌酯可湿性粉剂对油菜菌核病的防效(％)The preventive effect (%) of table 122% Bacillus subtilis·Kysstrobin wettable powder on rape sclerotinia
药效实施例2Efficacy Example 2
将枯草芽孢杆菌(200亿活孢子/克)与醚菌酯30∶3(质量比)的比例配成33％可湿性粉剂，喷雾，应用于防治小麦白粉病，单独将枯草芽孢杆菌和醚菌酯配成上述可湿性粉剂作对比，结果见表2。The ratio of Bacillus subtilis (20,000,000,000 live spores/gram) and kresoxim-methyl 30:3 (mass ratio) is formulated into 33% wettable powder, sprayed, and applied to the prevention and treatment of wheat powdery mildew. Esters were formulated into the above wettable powders for comparison, and the results are shown in Table 2.
表233％枯草芽孢杆菌·醚菌酯可湿性粉剂对小麦白粉病的防效(％)The control effect (%) of table 233% Bacillus subtilis·Kysstrobin wettable powder to wheat powdery mildew
药效实施例3Efficacy Example 3
将枯草芽孢杆菌(200亿活孢子/克)与醚菌酯10∶70(质量比)的比例配成80％可湿性粉剂，喷雾，应用于防治水稻纹枯病，单独将枯草芽孢杆菌和醚菌酯配成上述可湿性粉剂作对比，结果见表3。The ratio of Bacillus subtilis (20,000,000,000 live spores/gram) and kresoxim-methyl 10:70 (mass ratio) is formulated into 80% wettable powder, sprayed, and applied to the control of rice sheath blight, separately Bacillus subtilis and ether Strostrobin was formulated into the above wettable powder for comparison, and the results are shown in Table 3.
表380％枯草芽孢杆菌·醚菌酯可湿性粉剂对水稻纹枯病的防效(％)Table 380% Bacillus subtilis Kresoxim-methyl wettable powder to the control effect of rice sheath blight (%)
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| CN2013103584503A CN103430962A (en) | 2013-08-14 | 2013-08-14 | Bacillus subtilis and kresoxim-methyl composition |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340972A (en) * | 2015-12-17 | 2016-02-24 | 唐睿 | Bactericidal composition containing bacillus subtilis and kresoxim-methyl |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006347885A (en) * | 2005-06-13 | 2006-12-28 | Kumiai Chem Ind Co Ltd | Agricultural / horticultural fungicide composition |
| CN102458130A (en) * | 2009-05-06 | 2012-05-16 | 巴斯夫欧洲公司 | A method for increasing the vigor and/or crop yield of agricultural plants under essentially non-existent pathogen pressure |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006347885A (en) * | 2005-06-13 | 2006-12-28 | Kumiai Chem Ind Co Ltd | Agricultural / horticultural fungicide composition |
| CN102458130A (en) * | 2009-05-06 | 2012-05-16 | 巴斯夫欧洲公司 | A method for increasing the vigor and/or crop yield of agricultural plants under essentially non-existent pathogen pressure |
Non-Patent Citations (1)
| Title |
|---|
| 彭迪 等: "枯草芽孢杆菌NJ-18与醚菌酯协同防治黄瓜白粉病初探", 《中国植物病害化学防治研究》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340972A (en) * | 2015-12-17 | 2016-02-24 | 唐睿 | Bactericidal composition containing bacillus subtilis and kresoxim-methyl |
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Application publication date: 20131211 |