CN103424363B - Non-rotating optically-active solution measuring instrument and adopt this measuring instrument to measure the method for optically-active solution specific rotation - Google Patents

Abstract

The invention discloses a non-rotating optical rotation solution measuring instrument and a method for measuring the optical rotation rate of an optical rotation solution by using the measuring instrument, relating to a method for measuring an optical rotation solution. The invention solves the problem that the existing instruments for measuring optically active substances need to continuously rotate the polarizer to determine the rotation angle of the vibration plane of the optically active solution when measuring the optically active rate of the solution. In the present invention, the light beam emitted by the light source element is incident on the polarizer, the linearly polarized light polarized by the polarizer is incident on the test tube containing the optical rotation solution, and the linearly polarized light after passing through the test tube containing the optical rotation solution is incident on the polarizer The linearly polarized light after being polarized by the polarizer is incident on the photosensitive surface of the image acquisition device, and the image acquisition device sends the collected image information to the PC. The polarization direction is different from the polarization direction of the beam in the beam image information of the untested solution to obtain the optical rotation θ of the solution to be tested, and finally the optical rotation of the solution to be tested is obtained. The invention is suitable for measuring optically active solutions.

Description

Non-rotating optical rotation solution measuring instrument and method for measuring optical rotation of optical solution using the measuring instrument

technical field

The invention relates to a method for measuring an optically active solution.

Background technique

After the linearly polarized light passes through the solution of certain substances, the vibration plane of the polarized light will rotate at a certain angle with the direction of light propagation as the axis. This phenomenon is called optical rotation. The angle of rotation is called the optical rotation of the substance. A polarimeter is usually used to measure the optical rotation of a substance.

Optically active substances can be divided into right-handed and left-handed. When viewed from the direction of light, if the vibrating surface rotates clockwise, it is called a right-handed substance; if the vibrating surface rotates counterclockwise, it is called a left-handed substance.

In the existing measurement of optically active substances, on the optical bench, the laser beam emitted by the semiconductor laser, the polarizer, and the probe of the optical power meter are adjusted to be of equal height and coaxial. Adjust the polarizer turntable to make the output polarized light the strongest (semiconductor laser emits partially polarized light), and then place the analyzer on the slider of the optical bench so that the analyzer and the polarizer are coaxial at the same height (The analyzer is parallel to the polarizer). Adjust the polarizer turntable so that the output light intensity from the polarizer is zero (generally it cannot be adjusted to zero, but can only be adjusted to the minimum). At this time, the light transmission axis of the polarizer and the light transmission axis of the polarizer are perpendicular to each other. Continue Adjust the detector turntable so that the output light intensity from the polarizer is zero or minimum again, and read the readings of the analyzer turntable when the light intensity is zero for these two times, and the difference should be 180°.

Put the sample tube (with optically active liquid inside) on the bracket, and use a white paper to observe whether the shape of the light spot of the polarized light entering the sample tube is the same as that of the light spot exiting the sample tube, so as to check whether the glass is coaxial with the laser beam. . Adjust the rotating disk of the polarizer to observe the optical rotation characteristics of the optical liquid, whether it is a right-handed solution or a left-handed solution.

When measuring, it is more difficult to determine the zero point correction value of the polarimeter, the optical rotation direction of the optically active substance, and calculate the specific optical rotation. Since the vibration plane of polarized light gradually rotates as the light moves forward in the optically active substance, traditional instruments for measuring optically active substances need to continuously rotate the analyzer to determine the rotation angle of the vibration plane of the optically active solution.

Contents of the invention

In order to solve the problem that the existing instruments for measuring optically active substances need to continuously rotate the polarizer to determine the rotation angle of the vibration surface of the optically active solution when measuring the optically active rate of the solution, the present invention proposes a non-rotating optically active solution measuring instrument and adopts the measuring instrument Method for measuring optically active solutions.

The non-rotating polarimetric solution measuring instrument of the present invention includes a light source element, a polarizer, a test tube, a test tube rack, a polarizer, an image acquisition device, and a PC;

The light beam emitted by the light source element enters the polarizer, and the linearly polarized light after being polarized by the polarizer enters the test tube containing the optically active solution, and the linearly polarized light after passing through the test tube containing the optically active solution enters the polarizer, and passes through the polarizer. The linearly polarized light after polarized by the polarizer is incident on the photosensitive surface of the image acquisition device, the image acquisition device sends the collected image information to the PC, the test tube is installed on the test tube rack, and the test tube is used to contain the solution to be tested, and The center of the test tube is on the same straight line as the optical axis of the polarized beam polarized by the polarizer.

Adopt the method for measuring optically active solution of above-mentioned device, the concrete steps of this method are:

Step 1. Turn on the light source element, the light beam of the light source is incident on the polarizer after being polarized by the polarizer, and the light beam after passing through the polarizer is incident on the photosensitive surface of the image acquisition device,

Step 2: The image acquisition device collects the image information of the incident light beam, and transmits the collected light beam image information to the PC, and the PC extracts and obtains the polarization direction of the light beam in the light beam image information.

Step 3, put the optical rotation solution to be tested into the test tube, and install the test tube on the test tube rack, and the light beam is incident on the test tube containing the optical rotation solution to be tested after being polarized by the polarizer;

Step 4, the polarized light is incident on the polarizer after passing through the test tube containing the solution to be tested, and the polarized light after passing through the polarizer is incident on the photosensitive surface of the image acquisition device, and the image acquisition device collects image information of the incident light beam;

Step 5, the image acquisition device transmits the collected beam image information to the PC, and the PC extracts and obtains the polarization direction of the beam in the beam image information;

Step 6, the PC makes a difference between the polarization direction of the beam in the beam image information obtained in step 5 and the polarization direction of the beam in the beam image information obtained in step 2, to obtain the optical rotation θ of the solution to be measured;

Step 7. Use the formula

${<span\; class="notranslate">\; [</span>\mathrm{<span\; class="notranslate">\; \&alpha;</span>}<span\; class="notranslate">\; ]</span>}_{\mathrm{<span\; class="notranslate">\; \&lambda;</span>}}^{\mathrm{<span\; class="notranslate">\; t</span>}}<span\; class="notranslate">\; =</span>\frac{\mathrm{<span\; class="notranslate">\; \&theta;</span>}}{\mathrm{<span\; class="notranslate">\; l</span>}<span\; class="notranslate">\; \&Center\; Dot;</span>\mathrm{<span\; class="notranslate">\; C</span>}}$

Calculate the optical rotation of the solution to be measured;

In the formula, The t in the upper right corner indicates the temperature during the experiment, unit: ℃, λ is the wavelength of the monochromatic light source used by the polarimeter, unit: nm, θ is the measured optical rotation, l is the length of the sample tube, unit: dm, C is Solution concentration, unit: g/100mL.

The present invention replaces the original polarizer with a polarizer to realize the visualization and real-time detection of linearly polarized light. In the process of solution detection, it is not necessary to continuously rotate the polarizer to detect that the linearly polarized light vibrates after passing through the optical solution. The rotation angle of the plane, and the polarization direction of the linearly polarized light can be displayed on the PC in real time, so as to realize the real-time observation of the dynamic change of the polarization state.

Description of drawings

Fig. 1 is a schematic structural view of the device of the present invention.

detailed description

Specific Embodiments 1. This embodiment is described in conjunction with FIG. 1. The non-rotating polarimetric solution measuring instrument described in this embodiment includes a light source element 1, a polarizer 2, a test tube 3, a test tube rack 4, and a polarizer 5. , image acquisition device 6 and PC 7;

The light beam emitted by the light source element 1 is incident on the polarizer 2, and the linearly polarized light after being polarized by the polarizer 2 is incident on the test tube 3 containing the optically active solution, and the linearly polarized light after passing through the test tube 3 containing the optically active solution is incident on the The polarizer 4, the polarized light developed by the polarizer 4 is incident on the photosensitive surface of the image acquisition device 6, and the image acquisition device 6 sends the collected image information to the PC 7, and the test tube 3 is installed on the test tube rack 4 Above, the test tube 3 is used to contain the solution to be tested, and the center of the test tube 3 is on the same straight line as the optical axis of the polarized beam polarized by the polarizer 2 .

Replace the original polarizer with the polarizer. Realize the visualization and real-time detection of linearly polarized light. It can detect the angle of the incident ray polarized light and the rotation angle of the vibration plane after the linearly polarized light passes through the optical solution without continuous rotation, which reduces the error caused by mechanical rotation and improves the detection accuracy. , and the polarization direction of linearly polarized light can be displayed on the PC in real time. At the same time, it can be easily judged whether the substance in the sample tube is a left-handed substance or a right-handed substance.

Embodiment 2. This embodiment is a further description of the non-rotating polarimetric solution measuring instrument described in Embodiment 1. The light source element 1 is realized by a laser or an LED light source. Use a laser or LED light source as the incident light source, irradiate on the polarizer, pass through the polarizer, and obtain the polarized direction of the incident light on the PC.

Embodiment 3. This embodiment is a further description of the non-rotating polarimetric solution measuring instrument described in Embodiment 1 or Embodiment 2. The image acquisition device 6 is realized by a CCD camera.

The CCD camera is used to display the image of the linearly polarized light after 4 in real time, and the outgoing direction of the linearly polarized light can be accurately obtained through the software processing on the PC.

Embodiment 4. This embodiment is a method for measuring the optical rotation of an optically active solution using the non-rotating optical solution measuring instrument described in Embodiment 1. The specific steps of the method are:

Step 1: Turn on the light source element 1, the light beam of the light source is incident on the polarizer 5 after being polarized by the polarizer 2, and the light beam after passing through the polarizer 5 is incident on the photosensitive surface of the image acquisition device 6;

Step 2, the image acquisition device 6 collects the image information of the incident beam, and transmits the collected beam image information to the PC 7, and the PC 7 extracts and obtains the polarization direction of the beam in the beam image information;

Step 3, the solution to be tested is loaded into the test tube 3, and the test tube 3 is installed on the test tube rack 4, and the light beam is incident on the test tube containing the solution to be tested after being polarized by the polarizer 2;

Step 4, the polarized light is incident on the polarizer 5 after passing through the test tube 3 containing the solution to be tested, and the polarized light after passing through the polarizer 5 is incident on the photosensitive surface of the image acquisition device 6, and the image acquisition device 6 performs a process on the incident light beam. Image information collection;

Step 5, the image acquisition device 6 transmits the collected beam image information to the PC 7, and the PC 7 extracts and obtains the polarization direction of the beam in the beam image information;

Step 6, the PC 7 makes a difference between the polarization direction of the beam in the beam image information obtained in step 2 and the polarization direction of the beam in the beam image information obtained in step 5, to obtain the optical rotation θ of the solution to be measured;

Step 7. Use the formula

${<span\; class="notranslate">\; [</span>\mathrm{<span\; class="notranslate">\; \&alpha;</span>}<span\; class="notranslate">\; ]</span>}_{\mathrm{<span\; class="notranslate">\; \&lambda;</span>}}^{\mathrm{<span\; class="notranslate">\; t</span>}}<span\; class="notranslate">\; =</span>\frac{\mathrm{<span\; class="notranslate">\; \&theta;</span>}}{\mathrm{<span\; class="notranslate">\; l</span>}<span\; class="notranslate">\; \&Center\; Dot;</span>\mathrm{<span\; class="notranslate">\; C</span>}}$

Calculate the optical rotation of the solution to be tested,

In the formula, The t in the upper right corner indicates the temperature during the experiment, unit: ℃, λ is the wavelength of the monochromatic light source used by the polarimeter, unit: nm, θ is the measured optical rotation, l is the length of the sample tube, unit: dm, C is Solution concentration, unit: g/100mL.

In this embodiment, the original polarizer is replaced by a polarizer in a non-rotating polarimeter. During the test, when the linearly polarized light is incident, there is no need to adjust the polarizer, and the output image can be observed on the PC in real time through CCD imaging at the output end, and the polarization direction of the linearly polarized light can be obtained through image processing. At the same time, it can be easily judged whether the optically active substance in the sample tube is a levorotatory substance or a dextrorotatory substance.

Embodiment 5. This embodiment is a further description of the method for measuring the optical rotation of an optically active solution by the non-rotating optical solution measuring instrument described in Embodiment 4. In step 7, λ is 632.8 nm.

The invention replaces the original polarizer with a polarizer. Realize the visualization and real-time detection of linearly polarized light, without constantly rotating the analyzer to detect the angle of incident ray polarized light and the rotation angle of the vibration plane of linearly polarized light after passing through the optical solution, reducing the error caused by mechanical rotation The detection accuracy is improved, and the polarization direction of the linearly polarized light can be displayed on the PC in real time, thereby realizing the real-time observation of the dynamic change of the polarization state. At the same time, it can be easily judged whether the substance in the sample tube is a left-handed substance or a right-handed substance.

Claims (5)

1. Non-rotating polarimetric solution measuring instrument, it is characterized in that, described measuring instrument comprises light source element (1), polarizer (2), test tube (3), test tube rack (4), polarizer (5), Image acquisition device (6) and PC (7); The light beam emitted by the light source element (1) is incident on the polarizer (2), and the linearly polarized light after being polarized by the polarizer (2) is incident on the test tube (3) containing the solution to be tested. The linearly polarized light behind the test tube (3) is incident on the polarizer (4), and the linearly polarized light after being polarized by the polarizer (4) is incident on the photosensitive surface of the image acquisition device (6), and the image acquisition device ( 6) The collected image information is sent to the PC (7), the test tube (3) is installed on the test tube rack (4), the test tube (3) is used to contain the solution to be tested, and the center of the test tube (3) is connected to the center of the test tube (3). The optical axis of the polarized light beam after the polarizer (2) is on the same straight line; During the test, when the linearly polarized light enters the polarizer, the real-time observed image signal is sent to the PC through the image acquisition device, and the image is output on the PC in real time, and the polarization direction of the linearly polarized light is obtained through the PC image processing. Determine whether the optically active substance in the test tube is a levorotatory substance or a dextrorotatory substance. 2. The non-rotating polarimetric solution measuring instrument according to claim 1, characterized in that, the light source element (1) is realized by a semiconductor laser. 3. The non-rotating polarimetric solution measuring instrument according to claim 1 or 2, characterized in that the image acquisition device (6) is realized by a CCD camera. 4. adopt the method for non-rotating optical solution measuring instrument of claim 1 to measure optical rotation rate of optical solution, it is characterized in that, the concrete steps of this method are: Step 1: Turn on the light source element (1), the light beam is incident on the polarizer (5) after being polarized by the polarizer (2), and the beam after passing through the polarizer (5) is incident on the image acquisition device (6) photosensitive surface, Step 2, the image acquisition device (6) collects the image information of the incident light beam, and transmits the collected light beam image information to the PC (7), and the PC (7) extracts and obtains the polarization direction of the light beam in the light beam image information; Step 3, put the solution to be tested into the test tube (3), and install the test tube (3) on the test tube rack (4), the light beam is polarized by the polarizer (2) and then enters the test tube containing the solution to be tested ; Step 4, the polarized light is incident on the polarizer (5) after passing through the test tube (3) containing the solution to be tested, and the polarized light after passing through the polarizer (5) is incident on the photosensitive surface of the image acquisition device (6), The image acquisition device (6) performs image information acquisition on the incident light beam; Step 5, the image acquisition device (6) transmits the collected beam image information to the PC (7), and the PC (7) extracts and obtains the polarization direction of the beam in the beam image information; Step 6, the PC (7) makes a difference between the polarization direction of the beam in the beam image information obtained in step 5 and the polarization direction of the beam in the beam image information obtained in step 2, to obtain the optical rotation θ of the solution to be measured; Step 7. Use the formula ${<span\; class="notranslate">\; \&lsqb;</span>\mathrm{<span\; class="notranslate">\; \&alpha;</span>}<span\; class="notranslate">\; \&rsqb;</span>}_{\mathrm{<span\; class="notranslate">\; \&lambda;</span>}}^{\mathrm{<span\; class="notranslate">\; t</span>}}<span\; class="notranslate">\; =</span>\frac{\mathrm{<span\; class="notranslate">\; \&theta;</span>}}{\mathrm{<span\; class="notranslate">\; l</span>}<span\; class="notranslate">\; \&Center\; Dot;</span>\mathrm{<span\; class="notranslate">\; C</span>}}$ Calculate the optical rotation of the solution to be measured; In the formula, The t in the upper right corner indicates the temperature during the experiment, unit: ℃, λ is the wavelength of the monochromatic light source used by the non-rotating optical polarimeter, unit: nm, θ is the measured optical rotation, l is the length of the test tube, unit: dm , C is the solution concentration, unit: g/100mL. 5. The method for measuring the optical rotation of an optically active solution with a non-rotating optically active solution measuring instrument according to claim 4, wherein in step seven, λ is 632.8nm.